id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270594-62xotol3	He, Lei	Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus	2017-09-05		.txt	text/plain	4444	266	55	In this study, the viral structural protein 7 (VP7) polyclonal antibody was prepared with immunized mouse to explore the presence of small VP7 gene-encoded proteins in Bombyx mori cytoplasmic polyhedrosis virus. The replication of BmCPV genome in the cultured cells and midgut of silkworm was decreased by reducing the expression level of vp7 gene using RNA interference. In immunoprecipitation experiments, using a polyclonal antiserum directed against the VP7, one additional shorter band in BmCPV infected midguts was detected, and then the band was analyzed with mass spectrum (MS), the MS results showed thatone candidate interacted protein (VP7 voltage-dependent anion-selective channel-like isoform, VDAC) was identified from silkworm. A novel protein was detected from the overexpression of vp7 gene in the BmCPV infected cultured cells, with VP7 antibody. Total proteins from the BmCPV infected silkworm midguts (from the first day to the twelfth day) were also extracted, and detected with VP7 antibody.	./cache/cord-270594-62xotol3.txt	./txt/cord-270594-62xotol3.txt