id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284609-1q75zw6b	King, Andrew M.Q.	Recombination in RNA	1982-07-31		.txt	text/plain	4067	198	50	RNAase Tl fingerprints of virus RNA, prepared from representatives of each recombinant type, confirmed the approximate crossover sites that had been deduced from the inheritance of proteins. The possibility of genetic recombination in such viruses was first suggested many years ago by Hirst (1962) and Ledinko (1963) , who showed that infection of cells with a mixture of inhibitor-sensitive variants of poliovirus resulted in an enhanced yield of resistant progeny that were genetically stable. Second, we have obtained biochemical evidence of genetic recombination by crossing temperature-sensitive mutants of aphthovirus that possess second-site mutations affecting polypeptide charge. This paper describes a cross between two subtype strains of aphthovirus that has provided the first biochemical demonstration of genetic recombination at the level of RNA. Several spontaneous temperature-sensitive mutants of subtype OS were isolated and screened by electrofocusing polypeptides induced in virus-infected cells.	./cache/cord-284609-1q75zw6b.txt	./txt/cord-284609-1q75zw6b.txt