id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289192-1ecr16a3	Fujita, Motomichi	Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA	2019-02-01		.txt	text/plain	2374	160	54	title: Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA Here, we describe a simple, rapid, cost-effective, high-throughput method using a homogeneous time-resolved fluorescence (HTRF) assay [2] focusing on viral dsRNA. The dsRNA-HTRF assay may be a useful method for screening of antiviral agents against (+) ssRNA viruses. Moreover, we determined whether the dsRNA-HTRF assay could detect the concentration-and time-dependent signals. Therefore, to evaluate whether the dsRNA-HTRF assay can detect (+) ssRNA viruses, we focused on infection by picornaviruses represented by HRV. Schematic diagram of the double-stranded RNA (dsRNA) -homogeneous time-resolved fluorescence (HTRF)-based assay. In addition, we examined whether the dsRNA-HTRF assay could detect a single cycle of viral replication. H1-HeLa cells were infected with HRV-B14 at MOI of 10 to achieve single-cycle growth, and viral dsRNA was detected at 6 h post-infection using the dsRNA-HTRF assay ( Supplementary Fig. 2) , indicating that this assay could evaluate the single-cycle growth of HRV-B14.	./cache/cord-289192-1ecr16a3.txt	./txt/cord-289192-1ecr16a3.txt