id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291765-97lk5qfo	Eckerle, Lance D.	Effects of Mutagenesis of Murine Hepatitis Virus NSP1 and NSP14 on Replication in Culture	2006		.txt	text/plain	2187	105	50	To test the requirements for nsp1 and nsp14 in replication and to probe their functions, deletions or mutations were engineered into the viral genome in nsp1 and nsp14 and mutant viruses were analyzed for virus viability, replication, protein expression, and RNA synthesis. When infectious genome RNA containing these changes was electroporated into permissive cells, only the carboxy-terminal nsp1 deletion allowed recovery of an infectious mutant virus (nsp1 124-242). Based on the above results, systematic mutagenesis of clustered-charged residues was performed, both within the putative essential amino-terminal two-thirds of nsp1 as well as the dispensable carboxy-terminal third of the nsp1 protein domain. Deletions of P1-Gln residues in the flanking cleavage sites between nsp13-14 (VUSS6) and nsp14-15 (VUSS17) were engineered in the infectious clone cDNA and used to generate full-length genome RNA for electroporation into permissive cells.	./cache/cord-291765-97lk5qfo.txt	./txt/cord-291765-97lk5qfo.txt