id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293913-frkb8iso	Gao, Hong-Qiang	Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM	1996-12-31		.txt	text/plain	3624	176	53	Abstract The RNA polymerase gene of murine coronavirus MHV-JHM encodes a polyprotein of greater than 750 kDa. This polyprotein is proposed to be processed by two papain-like cysteine proteinases, PCP-1 and PCP-2, and a poliovirus 3C-like proteinase domain, 3C-pro, to generate protein products. To investigate which viral proteinase may be responsible for generating p72 and p65, we expressed the 5′-region of the MHV-JHM RNA polymerase gene including the two papain-like cysteine proteinase domains in an in vitro transcription/translation system and analyzed the translation products for proteolytic processing. To determine how p72 and p65 are generated from the polymerase polyprotein, we expressed cDNA clones encoding the 5'-end of ORF l a, including the 2 papain-like cysteine proteinase domains, and analyzed the translation reactions for the presence of proteolytic products.	./cache/cord-293913-frkb8iso.txt	./txt/cord-293913-frkb8iso.txt