id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295130-e7j7kac0	Moreno-Contreras, Joaquín	Saliva Sampling and Its Direct Lysis, an Excellent Option To Increase the Number of SARS-CoV-2 Diagnostic Tests in Settings with Supply Shortages	2020-09-22		.txt	text/plain	2906	122	58	In this study, we compared the RT-qPCR results from 253 paired samples obtained from saliva and swabs of ambulatory patients; the RNA in the swab samples was extracted using a commercial RNA purification kit, and the saliva samples were directly mixed with a lysis buffer, boiled, and used for the RT-qPCR protocol. To evaluate if saliva is a good source of viral RNA for the RT-qPCR, we determined the presence of the SARS-CoV-2 genome in paired saliva and swab samples from 253 ambulatory patients. Direct lysis of nasopharyngeal or oropharyngeal swab samples in viral transport medium using the QE buffer has been reported as a suitable method for direct RT-qPCR for SARS-CoV-2 detection, with rates similar to those of methods based on column purification (11, 15) .	./cache/cord-295130-e7j7kac0.txt	./txt/cord-295130-e7j7kac0.txt