id author title date pages extension mime words sentences flesch summary cache txt cord-301997-63160t7f Schwer, Beate Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader 1987-07-17 .txt text/plain 4743 236 55 We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by Sl mapping using vaccinia genomic DNA as a probe. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the "start site" determined by Sl mapping using vaccinia genomic DNA as a probe. As a negative control, we have performed primer extension with a dhfr primer and wild-type mRNA that does not contain dhfr sequences ( Figure 1, cDNA-RNA hybrids were incubated in the presence or absence of RNAase A at a final concentration of 10 Kg/ml and the hybrids were cap-selected by immunoprecipitation using a rabbit anti-m7G antiserum as described. After RNAase treatment-cap selection of the cDNA-RNA hybrids, we obtained the same results as for the wild-type translocated promoter-dhfr messengers (data not shown). ./cache/cord-301997-63160t7f.txt ./txt/cord-301997-63160t7f.txt