id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302980-2jlz4c58	Crucière, C.	Sequence and analysis of bovine enteritic coronavirus (F15) genome I.—Sequence of the gene coding for the nucleocapsid protein; analysis of the predicted protein	1988-03-31		.txt	text/plain	3914	386	70	Summary Sequences encoding the N protein of the bovine enteritic coronavirus-F15 strain (BECV-F15) have been cloned in PBR322 plasmid using cDNA produced by priming with oligo-dT on purified viral genomic RNA. The 3′-non-coding end of the gene has an 8-nucleotide sequence in common with the homologous genome areas of MHV, TGE and IBV viruses. The location of the insert along the viral genome was determined by Northern blot analysis: full length or purified products of insert restriction cleavage were hybridized with poly(A) § RNA extracted from infected or non-infected cells. We have determined, by cDNA cloning of BECV-F15 genomic RNA using an oligo-dT primer, a sequence of 1,710 nucleotides. For every coronavirus so far studied, the gene coding for the N protein is located at the 3'-end of the viral genome. Recently [11] it was described for the US Mebus strain of the related bovine corona virus (BCV), that the N protein gene was at the 3'-end of the viral genome.	./cache/cord-302980-2jlz4c58.txt	./txt/cord-302980-2jlz4c58.txt