id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303153-z7bdiuvx	Ulasli, Mustafa	Qualitative and quantitative ultrastructural analysis of the membrane rearrangements induced by coronavirus	2010-01-20		.txt	text/plain	8709	521	59	This approach has allowed us to establish that MHV induces the formation of six membranous rearrangements in the following order: DMVs, CMs, virions, LVCVs, TBs and CMSs. Importantly, we were able to show that most membrane rearrangements (LVCVs, TBs, CMSs and possibly CMs) observed in addition to the key structures in the infection (DMVs and virions) actually appear to be the consequence of a massive synthesis of viral proteins. In order to be able to correlate our EM and IEM analyses with the progression of a CoV infection inside the host cells, we first measured important known parameters that reflect the CoV life cycle: viral RNA replication/transcription, viral protein synthesis and secretion of progeny virus. In the centre of the DMV clusters, we frequently observed a small network of membranes with a diameter varying from 200 to 600 nm, which have recently been described in SARS-CoV-infected cells and called CMs [ Fig. 2A and B, arrowheads; (Knoops et al., 2008) .	./cache/cord-303153-z7bdiuvx.txt	./txt/cord-303153-z7bdiuvx.txt