id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-304283-nv4ret1f	Hung, Chuan-Fu	A novel siRNA validation system for functional screening and identification of effective RNAi probes in mammalian cells	2006-08-04		.txt	text/plain	7080	367	45	Since only a readily available short synthetic DNA fragment is needed for constructing this novel reporter-based siRNA validation system, this system not only provides a powerful strategy for screening highly effective siRNAs but also implicates in the use of RNAi for studying novel gene function in mammals. Since only a readily available short synthetic DNA fragment is needed for constructing both the targeting reporter and triggering siRNA expression vectors, this novel system should not only greatly facilitate large-scale lossof-function genetic screens in mammalian cells but also provide the basis for an improved approach to screen and identify the most potent siRNA for the purpose of the therapeutics. As the results shown in Fig. 5A , the siLMP1-2 exhibited no inhibition effects on pEGFP-3UTR-siLMP1-2-induced EGFP and pLuc+-3UTR-siLMP1-2-induced firefly luciferase expression as compared with the two standard positive controls, siEGFP and siLuc, indicating that the selected RNAi targeting sequence siLMP1-2 was inactive and non-functional.	./cache/cord-304283-nv4ret1f.txt	./txt/cord-304283-nv4ret1f.txt