id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306288-w43wec48	Jang, Sungho	RNA-based dynamic genetic controllers: development strategies and applications	2017-11-10		.txt	text/plain	4613	285	36	The aptamers and expression platforms were reassembled in a mix-and-match fashion to create chimeric riboswitches that retained their regulatory activities to turn off transcription upon ligand binding, and even combinations using artificial RNA aptamers were successful in regulating gene expression. Engineering of natural RNA-based regulation for dynamic control of gene expression requires detailed knowledge of each mechanism. Aptamers were placed upstream of IRES and several base-pairing sequences were designed to control the formation of critical structures (PK-III) depending on ligand binding (Figure 2b ). RNA molecules that regulate translation (IS10) or transcription (pT181) were combined with aptamers to control the formation of intramolecular structures depending on ligand binding [24] (Figure 2c ). Moreover, simultaneous utilization of high-throughput measurement of activity and next-generation sequencing analysis can not only screen and optimize dynamic RNA controllers, but also provide new design principles from sequence-structure-function relationships [14 ,15] .	./cache/cord-306288-w43wec48.txt	./txt/cord-306288-w43wec48.txt