id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306688-po4p1466	Wang, X.	Brome Mosaic Virus	2008-07-30		.txt	text/plain	4563	177	41	Among other advances, BMV was used to define the first ribosome binding sites in eukaryotic mRNAs, and to produce the first template-selective eukaryotic viral RNA-dependent RNA polymerase extract, the first infectious transcripts from cloned RNA virus cDNA, the first engineered RNA virus expression vectors expressing foreign genes, the first definition of subgenomic mRNA synthesis pathways and determinants, and the first demonstration of higher eukaryotic viral replication in yeast. In parallel to in vitro systems, cultured plant protoplasts provided a valuable substrate for in vivo replication studies due to their ability to be infected or transfected with nearly 100% efficiency with virions, virion RNAs, or in vitro transcripts from cloned viral cDNAs. For BMV, barley protoplast systems developed and refined by the groups of Okuno and Furusawa, Hall and others in the late 1970s have allowed studies of all aspects of BMV RNA replication, subgenomic RNA synthesis, progeny RNA encapsidation, and the like.	./cache/cord-306688-po4p1466.txt	./txt/cord-306688-po4p1466.txt