id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315054-kji2kfek	Chakraborty, Nabarun	Protocol Improvement for RNA Extraction From Compromised Frozen Specimens Generated in Austere Conditions: A Path Forward to Transcriptomics-Pathology Systems Integration	2020-07-22		.txt	text/plain	4462	214	45	In an effort to be economical with available resources, snap-frozen euthanized mice are the straightforward solution; however, this method increases the risk of temperature abuse during the thawing process at the beginning of the tissue collection. We found that prolonged immersion of snap frozen mouse carcass in 10% neutral buffered formalin at 4°C yielded minimal microscopic signs of ice crystallization and delivered tissues with histomorphology that is optimal for hematoxylin and eosin (H&E) staining and fixation on glass slides. An omics-pathology integration approach could be the most effective for phenometo-genome interpretation if omics assays are conducted using the particular tissue specimen, where injury signatures are informed by histopathology image analysis (Pathak and Dave, 2014; Yu et al., 2017) . These snap-frozen carcasses had to undergo onground freeze-thaw cycles before tissue collection, which is typically expected to compromise overall sample quality and make the histopathologic analysis challenging (Lyons et al., 1979; Pikal-Cleland et al., 2000) .	./cache/cord-315054-kji2kfek.txt	./txt/cord-315054-kji2kfek.txt