id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-318359-41h90h05	Irigoyen, Nerea	Ribosome profiling of the retrovirus murine leukemia virus	2018-01-22		.txt	text/plain	4488	238	54	Here, we report the first high resolution analysis of retrovirus gene expression through tandem ribosome profiling (RiboSeq) and RNA sequencing (RNASeq) of MuLV-infected cells. Translation may proceed contiguously from this codon through the gag ORF; thus, presumably, giving rise to an alternative Glyco-Gag isoform which is N-terminally extended by a further 12 amino acids (MELTSSEHPAAT, assuming GUG is decoded by Met-tRNA i ) relative to the canonical Glyco-Gag. The GUG codon is present in some but not all MuLV strains, and is not well conserved among other gammaretroviral lineages for which sequence data are currently available (Additional file 1: Fig. S3-highlighted in green) , although some sequences have alternative nearby potential non-AUG initiation sites. Our data indicate the existence of new translation initiation sites, new translated short ORFs and the first measurement of the gag-pol stop codon readthrough efficiency in the context of the full-length virus genome during infection.	./cache/cord-318359-41h90h05.txt	./txt/cord-318359-41h90h05.txt