id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319649-d6dqr03e	Yang, Jie	A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing	2013-12-05		.txt	text/plain	7876	375	53	Here, we expressed VP5 from type 5 Helicoverpa armigera cypovirus (HaCPV-5) in a eukaryotic system and determined that this VP5 possesses RNA chaperone-like activity, which destabilizes RNA helices and accelerates strand annealing independent of ATP. In this study, we expressed HaCPV-5 VP5 in a eukaryotic expression system and determined that this CPV VP5 possesses an RNA chaperone-like activity to ATP-independently destabilize RNA helices and accelerate strand annealing. Moreover, we found that HaCPV-5 VP5 could facilitate the transcription initiation of an alternative polymerase (i.e. reverse transcriptase) through a CPV panhandle-structured RNA template, thereby strongly suggesting a direct role of the RNA chaperone activity of VP5 in the initiation of cypoviral dsRNA replication. In the family Reoviridae, CPV VP5 may not be the only RNA chaperone, as rotavirus nonstructural protein 2 (NSP2), which is a multifunctional enzyme involved in rotaviral dsRNA replication, was previously shown to contain ATP-independent nucleic acid helix-destabilizing activity (45) .	./cache/cord-319649-d6dqr03e.txt	./txt/cord-319649-d6dqr03e.txt