id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329366-xuszdrsa	Hackbart, Matthew	Coronavirus endoribonuclease targets viral polyuridine sequences to evade activating host sensors	2020-04-07		.txt	text/plain	7187	445	57	In this study, we show that CoV EndoU activity limits the abundance and length of the polyuridine (polyU) extension on 5′-polyU-containing, negative-sense (PUN) RNAs for both the beta-CoV mouse hepatitis virus strain A59 (MHV-A59) and the alpha-CoV PEDV. Overall, we propose a mechanism for EndoU, which is to cleave polyU sequences from PUN RNAs, thus limiting the formation of a PAMP and impeding the ability of MDA5 to activate the innate immune response to infection. EndoU Activity Limits Abundance and Length of PUN RNAs. Previous studies showed that the 5′ end of the CoV negative-sense RNA contains polyU extensions (35) , and that EndoU cleaves at uridine residues (22, 25, (27) (28) (29) (30) . We found that the products generated from positive-sense RNA were similar between wild-type and EndoUmut viruses, consistent with our previous results indicating that the polyA tail is not cleaved by EndoU activity (Fig. 5C ).	./cache/cord-329366-xuszdrsa.txt	./txt/cord-329366-xuszdrsa.txt