id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329687-vhi4tbnc	Verdugo, C.	A comparative evaluation of dye-based and probe-based RT-qPCR assay for the screening of SARS-CoV-2 using individual and pooled-sample testing.	2020-06-03		.txt	text/plain	3497	207	61	title: A comparative evaluation of dye-based and probe-based RT-qPCR assay for the screening of SARS-CoV-2 using individual and pooled-sample testing. However, the most efficient system for massive surveillance, the pre-RNA extraction pooling approach, was obtained with the probe-based assay in test up to 10 samples adding 13.5 uL of RNA template. In this study, we compared a dye-based and probe-based real-time RT-qPCR assay for the economic and rapid detection and quantification of SARS-CoV-2 in human samples by individual and pooling testing. Our results showed that using the dye-based assay describe here, the SARS-CoV-2 can be detected up to 50 viral copies (a dilution of 10 copies/µL) of the RNA template, showing a similar analytical sensitivity obtained with the probe-based standard technique ( Table 2) showed no significant differences in CT values between pooled and individual samples, suggesting that sensitivity was not affected by pooling specimens, regardless of the viral load (Wacharapluesadee et al, 2020).	./cache/cord-329687-vhi4tbnc.txt	./txt/cord-329687-vhi4tbnc.txt