id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329707-89zyu8bl	Zhang, Xue	Inhibition of SARS-CoV Gene Expression by Adenovirus-Delivered Small Hairpin RNA	2006-11-30		.txt	text/plain	3212	210	54	We constructed recombinant adenoviral vectors that can express shRNAs, which inhibited the expression of SARS-CoV genes effectively in mammalian cells. METHODS: In this study, we designed several plasmids that express small hairpin RNA molecules (shRNA) specifically targeting to the genes encoding for the SARS-CoV nucleocapsid (N) protein and envelope (E) protein, respectively. The effects of adenovirus-delivered small hairpin RNA on SARS-CoV gene expression were determined by RT-PCR, Western blot, and luciferase activity assays. RESULTS: The levels of viral mRNAs and viral proteins of the targets were significantly decreased or completely inhibited in cell lines after being infected with the recombinant adenoviruses that expressed specific shRNA molecules. CONCLUSIONS: Since many cell types can be efficiently infected by adenovirus, recombinant adenoviruses could serve as an alternative powerful tool for shRNA delivery and for gene suppression, especially when the targeted cells are resistant to transfection by DNA or RNA.	./cache/cord-329707-89zyu8bl.txt	./txt/cord-329707-89zyu8bl.txt