id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332710-2s14knw6	Lai, Michael M.C.	Recombination in large RNA viruses: Coronaviruses	1996-12-31		.txt	text/plain	4204	236	42	The capacity of coronaviruses to undergo recombination may be related to its mRNA transcription mechanism, which involves discontinuous RNA synthesis, suggesting the nonprocessive nature of the viral polymerase. The first coronavirus recombinant was isolated by coinfecting temperature-sensitive (ts) mutants of two mouse hepatitis virus (MHV) strains, A59 and JHM, and selecting progeny viruses which grew at the nonpermissive temperature. In contrast, clear-cut evidence of recombination has been obtained for natural isolates of avian infectious bronchitis virus (IBV), many of which have recombination between different strains in the spike protein gene or the 3'-end of viral RNA. 33 In this case, the viral RNA containing the sequence of the transfected RNA fragments was detected by reverse transcription-polymerase chain reaction (RT-PCR), although the actual recombinants could not be isolated because of lack of selection markers.	./cache/cord-332710-2s14knw6.txt	./txt/cord-332710-2s14knw6.txt