id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333636-h2sg6shp	Kochan, G.	Characterization of the RNA-binding activity of VP3, a major structural protein of Infectious bursal disease virus	2003		.txt	text/plain	5975	334	53	The histidine-tagged VP3 was affinity-purified and used to study its ability to bind RNA molecules using three complementary methods: (i) Northwestern blotting; (ii) binding of VP3 protein-RNA complexes to nitrocellulose membranes; and (iii) electrophoretic mobility shift assays. Briefly, increasing amounts (from 5 × 10 −2 mM to 5 × 10 −6 mM) of purified VP3 or BSA, used as a control for non-specific interaction, were mixed with 10 6 cpm of 32 P-labeled ssRNA probe B (5 × 10 −6 mM) in binding reaction buffer (50 mM Tris, 150 mM KCl, 150 mM NaCl 10% Glycerol, 0,01% Triton X 100), in 16 µl final volume, so the VP3/RNA molar ratio in these experiments varied from 10 4 to 1. Competition assays based on nitrocellulose binding were carried out using virus-derived radiolabeled ssRNA probes together with increasing concentrations of non-radioactive competitor RNA and DNA probes.	./cache/cord-333636-h2sg6shp.txt	./txt/cord-333636-h2sg6shp.txt