id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333979-bx2xspbe	Kalikiri, Mahesh K R	High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads	2020-04-11		.txt	text/plain	2029	102	52	title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads We developed a method using a widely available lysis buffer coupled with solid-phase reverse immobilization (SPRI) beads to extract viral RNA from swabs collected in viral transport medium (VTM) which can be performed manually or on a Hamilton STAR liquid-handling robot. Retrospective, residual nasopharyngeal specimens that previously tested positive or negative for respiratory syncytial virus (RSV) using the Cepheid GeneXpert Flu/RSV kit or the Fast Track Diagnostics Respiratory Pathogens 21 PCR (FTD-RESP21) were extracted with our new approach on a Hamilton STAR liquid handling robot with 8 CO-RE channels. When applying these conditions to 204 clinical samples, we only observed a 2.5% inhibition of the RT-qPCR, indicating that our method is suitable for large-scale use in testing laboratories. In this manuscript, we demonstrate the feasibility of using reagents commonly available in molecular biology and next-generation sequencing laboratories for the extraction of SARS-CoV-2 viral RNA for the diagnosis of COVID-19.	./cache/cord-333979-bx2xspbe.txt	./txt/cord-333979-bx2xspbe.txt