id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337508-nfzaw8gg	Kirkland, P.D.	The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2	2020-10-05		.txt	text/plain	2708	134	54	authors: Kirkland, P.D.; Frost, M.J. title: The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 With the widespread introduction of molecular based diagnostic assays, especially real time PCR (qPCR), studies have been undertaken to evaluate the stability of viruses in VTMs, particularly in commercially prepared products, while being held at a range of temperatures. After becoming aware of concerns of variable results for the same samples in different assays, we initiated a study to compare the stability of SARS-CoV-2 RNA in several commercially manufactured VTMs and an in-house product. The results of these studies clearly indicate that the commercially prepared VTM solutions have had an adverse impact on the ability to detect both SARS-CoV-2 and influenza RNA. The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2 and other viruses	./cache/cord-337508-nfzaw8gg.txt	./txt/cord-337508-nfzaw8gg.txt