id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338345-mr1orklo	Adedeji, Adeyemi O.	Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase	2016-09-07		.txt	text/plain	4253	233	57	Unless stated otherwise, for this specific experiment, we used the 5=-RNA-20 partially duplex substrate (see Fig. S1 in the supplemental material), since previous coronavirus helicase, i.e., SARS-CoV nsp13, was shown to have a 5=-to-3= directionality (29) . To determine whether nucleic acid unwinding by M-nsp13 depends on the loading strand length and to determine the minimum length of the 5= singlestranded overhang required for efficient helicase activity, we designed five partially duplex RNA substrates with single-stranded 5= ends of various lengths (between 0 and 20 bases) (see Fig. S1 in the supplemental material; Fig. 6 ) but with dsRNA duplex regions of the same length. Overall, these results suggest that M-nsp13's ability to unwind the 5=-RNA-20 partially duplex substrate is likely due to residual cellular ATP purified with the helicase as well as to a long overhang length, which enhances M-nsp13's unwinding activity.	./cache/cord-338345-mr1orklo.txt	./txt/cord-338345-mr1orklo.txt