id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338358-ppjxo2di	Whitworth, Kristin M.	Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs	2016-10-15		.txt	text/plain	4959	267	55	title: Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs Pairs of CRISPR guide RNAs that flanked the start codon and polyadenylated Cas9 were co-injected into the cytoplasm of zygotes and cultured in vitro to the blastocyst stage. In a previous study, CRISPR/Cas9 RNA injection of pig zygotes resulted in 100 % of the piglets having biallelic DNA edits of the targeted CD163 or CD1D gene (Whitworth et al. Zygote injection with CRISPR/Cas9 guide RNA was used to efficiently (100 %) create pigs with a biallelic edit of the TMPRSS2 gene for use as a biomedical model (Fig. 2e, f) . The sex ratio measured in the resulting blastocysts and piglets was not significantly affected by in vitro culture or CRISPR/Cas9 RNA injection.	./cache/cord-338358-ppjxo2di.txt	./txt/cord-338358-ppjxo2di.txt