id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341541-3l6tjf3t	Hajijafari Anaraki, Mozafar	Molecular characterization of infectious bronchitis virus based on RNA‐dependent RNA polymerase gene	2020-05-26		.txt	text/plain	1276	87	49	title: Molecular characterization of infectious bronchitis virus based on RNA‐dependent RNA polymerase gene Extensive rate of variations in in spike glycoprotein subunit gene of infectious bronchitis virus (IBV) caused challenges for counting variants for differentiation of infected from vaccinated birds and addressing the variants of unknown significance. The study aimed at investigating the possibility use of RNA‐dependent RNA polymerase gene (RdRp) as a target for molecular characterization of IBV strains in Iran. Phylogenetic analysis of RdRp gene sequences resulted in clustering the IBV strains related to each area. Using RdRp, as a genetic marker eliminates the challenges arise from the enormous variations that making difficult the discrimination between field and vaccine strains as well as affiliation of certain variants to various geographical areas. Based on the RT-PCR detection and sequence analysis of IBV RdRp gene, an overall prevalence of field strains estimated as 44.2%.	./cache/cord-341541-3l6tjf3t.txt	./txt/cord-341541-3l6tjf3t.txt