id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345204-ch0e6lzl	Scarlata, S.	Design Of A Rapid And Reversible Fluorescence Assay To Detect COVID-19 And Other Pathogens	2020-10-05		.txt	text/plain	2917	172	59	The method uses fluorescent sensors (i.e. molecular beacons) designed to detect COVID-19 RNA or any RNA of interest, concurrent with an internal control without the need for amplification. The molecular beacons are stem-loop structures in which a ~10 nucleotide loop region has the complementary sequence of a region of the target RNA, and a fluorophore and quencher are placed on the 5' and 3' ends of the stem. Here, we designed a COVID-19 beacon that is completely quenched in its native form and undergoes a 50-fold increase in fluorescence when exposed to nanomolar amounts of synthetic viral oligonucleotide. Fluorescence increases from beacon responses signals are rapid and can be reversed by the addition of inexpensive ssDNA with a sequence identical to the loop region, or high salt if attached to a matrix.	./cache/cord-345204-ch0e6lzl.txt	./txt/cord-345204-ch0e6lzl.txt