id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345413-bsd32j8r	Terada, Yutaka	Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663	2019-08-02		.txt	text/plain	7979	438	56	To determine viral RNA replication in A72 cells, A72, MDCK, DH82, and Fcwf-4 cells were infected with rC3663-Nluc virus at an MOI of 0.01, followed by real-time RT-PCR analysis of RNA extracted at 24, 48, and 72 hpi. Next, we determined the levels of production of infectious virus particles from rC3663-Nluc-infected A72 cells by collecting the culture supernatants at 24, 48, and 72 hpi and measuring viral titers by plaque assays with Fcwf-4 cells (Fig. 3E ). Thus, to determine expression levels of sg N mRNA (sg mRNA6), total RNAs extracted from rC3663 virus-infected or mock-infected Fcwf-4 and A72 cells at 48 and 72 hpi were subjected to Northern blot analysis with a specific type I FCoV 3= untranslated region (3=UTR) probe (Fig. 5A) . We further examined the expression levels of viral sg mRNAs in cells infected with the parental C3663 strain or type I FCoV strain Yayoi using Northern blot analysis with specific RNA probes against the 3=-UTR.	./cache/cord-345413-bsd32j8r.txt	./txt/cord-345413-bsd32j8r.txt