id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348815-lthz75oc	Kurreck, Jens	RNA Interference: From Basic Research to Therapeutic Applications	2009-01-19		.txt	text/plain	14116	846	56	Successful applications in animal models have already led to the initiation of RNAi‐based clinical trials as a new therapeutic option.[Image: see text] Only ten years ago Andrew Fire and Craig Mello were able to show that double‐stranded RNA molecules could inhibit the expression of homologous genes in eukaryotes. This Review provides an overview of the molecular processes involved, with a particular focus on the posttranscriptional inhibition of gene expression in mammalian cells, the possible applications in research, and the results of the first clinical studies. Antisense and RNAi strategies have many things in common, such as the necessity to identify suitable binding sequences on the target RNA, the stabilization of the oligonucleotide by chemical modification, or the transport of the negatively charged polymer across the cell membrane. [19] There are, however, important differences between the two technologies: Antisense oligonucleotides are single-stranded (modified) DNA molecules, which primarily induce the cleavage of the target RNA in the cell nucleus by activation of RNase H.	./cache/cord-348815-lthz75oc.txt	./txt/cord-348815-lthz75oc.txt