id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350040-e8q7wq0h	Aronin, N	Target selectivity in mRNA silencing	2006-02-16		.txt	text/plain	6614	503	58	Here is limned a roadmap to explain RISC assemblyhow there are two types of RNAi, one of which is applicable to humans; how thermodynamic properties of siRNA direct strand selection to confer full RNAi activity; how RISC proteins direct siRNA presentation to its target mRNA; and how these principles can be used to design selective and functional siRNAs. Innate mRNA silencing in mammals is currently the province of microRNAs, single stranded, small RNAs that block translation with short-term survival of target mRNA. Not all siRNAs are active, however, even when their guide strands have perfect complementarity to target mRNAs. Designing siRNAs with single nucleotide specificity requires the guide strand to be incorporated into RISC, in preference to the passenger strand. The complexity of siRNA design is that small changes in siRNA:mRNA complementarity can have profound effects on RNAi effectiveness; mismatches might have few consequences or, as shown above, improve silencing by strand selection.	./cache/cord-350040-e8q7wq0h.txt	./txt/cord-350040-e8q7wq0h.txt