id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350600-73q8mve4	Myint, S.	Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization	2005-12-09		.txt	text/plain	1996	126	61	A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridization. In this paper we describe a specific and sensitive test to detect one of these major groups, HCV 2293, in nasal washings. Briefly, using a method based on that of Gubler and Hoffmann [19831, cDNAs were generated from HCV 2293 RNA isolated from infected C16 cells [Phillpotts, 19831. The results of virus titration and probing of nasal washings from seven volunteers are presented in Table I . Three of the seven volunteers suffered a cold, and all three volunteers had detectable coronavirus RNA in their nasal washings. The results we have obtained show that the detection of HCV 2293 infection by nucleic acid hybridisation is a reliable and specific method. Hybridisation to known quantities of HCV 2293 RNA showed that less than 1 ng of virus-specific RNA from C16 cells infected with HCV 2293 could be detected.	./cache/cord-350600-73q8mve4.txt	./txt/cord-350600-73q8mve4.txt