id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350906-ew04zzh6	Khambhati, Khushal	Current progress in CRISPR‐based diagnostic platforms	2018-10-26		.txt	text/plain	2266	136	49	10 East-Seletsky et al 10 have taken the advantage and demonstrated the use of Cas13 for the detection of 0.01 nM of λRNA with high specificity, using the signals generated from fluorophore-quencher based reporter RNA molecule ( Figure 1 ). The resultant amplified DNA sequence is then subjected to in vitro T7 transcription, and produced RNA molecule is detected by Cas13-guided reporter assay. 11 For improved multiplexed detection, Cas12a has been used along with Cas13 RNAse. 11 F I G U R E 1 Schematic representation of CRISPR-based diagnostic tool, in the presence of the appropriate gRNA, Cas13 recognizes the target RNA sequence that is complementary. The produced RNA molecule can be recognized via fluorescence signals that are generated by the reporter molecule upon target recognition by the Cas13 RNAse. 4 The cleaved reporter molecule can also be detected in the form of bands through a lateral flow assay.	./cache/cord-350906-ew04zzh6.txt	./txt/cord-350906-ew04zzh6.txt