id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-351864-zozrj7w5	Chappleboim, A.	ApharSeq: An Extraction-free Early-Pooling Protocol for Massively Multiplexed SARS-CoV-2 Detection	2020-08-13		.txt	text/plain	5782	321	57	Most current tests for SARS-CoV-2 are based on RNA extraction followed by quantitative reverse-transcription PCR assays that involve a separate RNA extraction and qPCR reaction for each sample with a fixed cost and reaction time. Our workflow, ApharSeq, includes a fast and cheap RNA capture step, that is coupled to barcoding of individual samples, followed by sample-pooling prior to the reverse transcription, PCR and massively parallel sequencing. Briefly, we show ( Figure 1 ) that we can introduce barcoded and target-specific reverse transcription primers to the samples, allowing them to hybridize to target RNA molecules already in the lysis buffer, or after a brief RNA cleanup step. Observing the target sequence directly allowed us to identify viral sequence variations in some cases ( Figure 2D ).Cross-Sample Contamination is minimal When pooling samples early on in the protocol, the main concern is that RNA molecules will be erroneously tagged due to residual free primers, or due to other artifacts during RT, PCR, or sequencing.	./cache/cord-351864-zozrj7w5.txt	./txt/cord-351864-zozrj7w5.txt