id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-356115-vblgotjn	Sawicki, Stanley G	Functional and Genetic Analysis of Coronavirus Replicase-Transcriptase Proteins	2005-12-09		.txt	text/plain	10631	458	52	The coronavirus replicase-transcriptase complex is an assembly of viral and cellular proteins that mediate the synthesis of genome and subgenome-sized mRNAs in the virus-infected cell. Here, we report a genetic and functional analysis of 19 temperature-sensitive (ts) mutants of Murine hepatitis virus MHV-A59 that are unable to synthesize viral RNA when the infection is initiated and maintained at the non-permissive temperature. We focused our phenotypic analysis on the eight MHV-A59 ts mutants that had been genotyped and began by measuring ''total'' viral RNA synthesis in infected cells prior to and following shift from the permissive to the non-permissive temperature. This phenotype resembled that seen with MHV-A59-infected cells treated with CH, and we conclude that the complementation group I mutants are defective in their ability to form active replicase-transcriptase complexes at 40 8C but retain the positive-strand synthesis activity of the complexes formed at the permissive temperature.	./cache/cord-356115-vblgotjn.txt	./txt/cord-356115-vblgotjn.txt