id sid tid token lemma pos cord-016755-ye37z5h9 1 1 key key NN cord-016755-ye37z5h9 1 2 : : : cord-016755-ye37z5h9 1 3 cord-016755-ye37z5h9 cord-016755-ye37z5h9 NNP cord-016755-ye37z5h9 1 4 authors author NNS cord-016755-ye37z5h9 1 5 : : : cord-016755-ye37z5h9 1 6 Li Li NNP cord-016755-ye37z5h9 1 7 , , , cord-016755-ye37z5h9 1 8 Jiandong Jiandong NNP cord-016755-ye37z5h9 1 9 ; ; : cord-016755-ye37z5h9 1 10 Li Li NNP cord-016755-ye37z5h9 1 11 , , , cord-016755-ye37z5h9 2 1 Dexin Dexin NNP cord-016755-ye37z5h9 2 2 title title NN cord-016755-ye37z5h9 2 3 : : : cord-016755-ye37z5h9 2 4 The the DT cord-016755-ye37z5h9 2 5 Discovery Discovery NNP cord-016755-ye37z5h9 2 6 Process Process NNP cord-016755-ye37z5h9 2 7 of of IN cord-016755-ye37z5h9 2 8 SFTS SFTS NNP cord-016755-ye37z5h9 2 9 in in IN cord-016755-ye37z5h9 2 10 China China NNP cord-016755-ye37z5h9 2 11 date date NN cord-016755-ye37z5h9 2 12 : : : cord-016755-ye37z5h9 2 13 2019 2019 CD cord-016755-ye37z5h9 2 14 - - SYM cord-016755-ye37z5h9 2 15 10 10 CD cord-016755-ye37z5h9 2 16 - - SYM cord-016755-ye37z5h9 2 17 12 12 CD cord-016755-ye37z5h9 2 18 journal journal NN cord-016755-ye37z5h9 2 19 : : : cord-016755-ye37z5h9 2 20 Severe severe JJ cord-016755-ye37z5h9 2 21 Fever fever NN cord-016755-ye37z5h9 2 22 with with IN cord-016755-ye37z5h9 2 23 Thrombocytopenia Thrombocytopenia NNP cord-016755-ye37z5h9 2 24 Syndrome Syndrome NNP cord-016755-ye37z5h9 2 25 DOI DOI NNP cord-016755-ye37z5h9 2 26 : : : cord-016755-ye37z5h9 2 27 10.1007/978 10.1007/978 CD cord-016755-ye37z5h9 2 28 - - HYPH cord-016755-ye37z5h9 2 29 981 981 CD cord-016755-ye37z5h9 2 30 - - HYPH cord-016755-ye37z5h9 2 31 13 13 CD cord-016755-ye37z5h9 2 32 - - HYPH cord-016755-ye37z5h9 2 33 9562 9562 CD cord-016755-ye37z5h9 2 34 - - HYPH cord-016755-ye37z5h9 2 35 8_2 8_2 CD cord-016755-ye37z5h9 2 36 sha sha NN cord-016755-ye37z5h9 2 37 : : : cord-016755-ye37z5h9 2 38 ed3c8532f3a46373186eddf080fc4e106a2ef6c0 ed3c8532f3a46373186eddf080fc4e106a2ef6c0 IN cord-016755-ye37z5h9 2 39 doc_id doc_id CD cord-016755-ye37z5h9 2 40 : : : cord-016755-ye37z5h9 2 41 16755 16755 CD cord-016755-ye37z5h9 2 42 cord_uid cord_uid NNS cord-016755-ye37z5h9 2 43 : : : cord-016755-ye37z5h9 3 1 ye37z5h9 ye37z5h9 NNP cord-016755-ye37z5h9 3 2 Heightened Heightened NNP cord-016755-ye37z5h9 3 3 surveillance surveillance NN cord-016755-ye37z5h9 3 4 of of IN cord-016755-ye37z5h9 3 5 acute acute JJ cord-016755-ye37z5h9 3 6 febrile febrile JJ cord-016755-ye37z5h9 3 7 illness illness NN cord-016755-ye37z5h9 3 8 in in IN cord-016755-ye37z5h9 3 9 China China NNP cord-016755-ye37z5h9 3 10 since since IN cord-016755-ye37z5h9 3 11 2004 2004 CD cord-016755-ye37z5h9 3 12 led lead VBD cord-016755-ye37z5h9 3 13 to to IN cord-016755-ye37z5h9 3 14 the the DT cord-016755-ye37z5h9 3 15 identification identification NN cord-016755-ye37z5h9 3 16 of of IN cord-016755-ye37z5h9 3 17 a a DT cord-016755-ye37z5h9 3 18 severe severe JJ cord-016755-ye37z5h9 3 19 fever fever NN cord-016755-ye37z5h9 3 20 with with IN cord-016755-ye37z5h9 3 21 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 3 22 syndrome syndrome NN cord-016755-ye37z5h9 3 23 ( ( -LRB- cord-016755-ye37z5h9 3 24 SFTS SFTS NNP cord-016755-ye37z5h9 3 25 ) ) -RRB- cord-016755-ye37z5h9 3 26 with with IN cord-016755-ye37z5h9 3 27 unknown unknown JJ cord-016755-ye37z5h9 3 28 etiology etiology NN cord-016755-ye37z5h9 3 29 . . . cord-016755-ye37z5h9 4 1 Sporadic sporadic JJ cord-016755-ye37z5h9 4 2 patients patient NNS cord-016755-ye37z5h9 4 3 hospitalized hospitalize VBN cord-016755-ye37z5h9 4 4 with with IN cord-016755-ye37z5h9 4 5 SFTS SFTS NNP cord-016755-ye37z5h9 4 6 in in IN cord-016755-ye37z5h9 4 7 2009 2009 CD cord-016755-ye37z5h9 4 8 and and CC cord-016755-ye37z5h9 4 9 2010 2010 CD cord-016755-ye37z5h9 4 10 were be VBD cord-016755-ye37z5h9 4 11 identified identify VBN cord-016755-ye37z5h9 4 12 and and CC cord-016755-ye37z5h9 4 13 serum serum NN cord-016755-ye37z5h9 4 14 samples sample NNS cord-016755-ye37z5h9 4 15 were be VBD cord-016755-ye37z5h9 4 16 collected collect VBN cord-016755-ye37z5h9 4 17 . . . cord-016755-ye37z5h9 5 1 Sequence sequence NN cord-016755-ye37z5h9 5 2 from from IN cord-016755-ye37z5h9 5 3 a a DT cord-016755-ye37z5h9 5 4 novel novel JJ cord-016755-ye37z5h9 5 5 species species NN cord-016755-ye37z5h9 5 6 of of IN cord-016755-ye37z5h9 5 7 phlebovirus phlebovirus NNP cord-016755-ye37z5h9 5 8 was be VBD cord-016755-ye37z5h9 5 9 identified identify VBN cord-016755-ye37z5h9 5 10 by by IN cord-016755-ye37z5h9 5 11 sequence sequence NN cord-016755-ye37z5h9 5 12 independent independent JJ cord-016755-ye37z5h9 5 13 single single JJ cord-016755-ye37z5h9 5 14 primer primer NN cord-016755-ye37z5h9 5 15 amplification amplification NN cord-016755-ye37z5h9 5 16 ( ( -LRB- cord-016755-ye37z5h9 5 17 SISPA SISPA NNP cord-016755-ye37z5h9 5 18 ) ) -RRB- cord-016755-ye37z5h9 5 19 from from IN cord-016755-ye37z5h9 5 20 the the DT cord-016755-ye37z5h9 5 21 serum serum NN cord-016755-ye37z5h9 5 22 of of IN cord-016755-ye37z5h9 5 23 a a DT cord-016755-ye37z5h9 5 24 patient patient NN cord-016755-ye37z5h9 5 25 with with IN cord-016755-ye37z5h9 5 26 SFTS SFTS NNP cord-016755-ye37z5h9 5 27 . . . cord-016755-ye37z5h9 6 1 The the DT cord-016755-ye37z5h9 6 2 virus virus NN cord-016755-ye37z5h9 6 3 was be VBD cord-016755-ye37z5h9 6 4 isolated isolate VBN cord-016755-ye37z5h9 6 5 in in IN cord-016755-ye37z5h9 6 6 Vero Vero NNP cord-016755-ye37z5h9 6 7 cell cell NN cord-016755-ye37z5h9 6 8 culture culture NN cord-016755-ye37z5h9 6 9 and and CC cord-016755-ye37z5h9 6 10 its -PRON- PRP$ cord-016755-ye37z5h9 6 11 complete complete JJ cord-016755-ye37z5h9 6 12 genome genome NN cord-016755-ye37z5h9 6 13 sequence sequence NN cord-016755-ye37z5h9 6 14 was be VBD cord-016755-ye37z5h9 6 15 determined determine VBN cord-016755-ye37z5h9 6 16 , , , cord-016755-ye37z5h9 6 17 only only RB cord-016755-ye37z5h9 6 18 distantly distantly RB cord-016755-ye37z5h9 6 19 related relate VBN cord-016755-ye37z5h9 6 20 to to IN cord-016755-ye37z5h9 6 21 other other JJ cord-016755-ye37z5h9 6 22 known know VBN cord-016755-ye37z5h9 6 23 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 6 24 . . . cord-016755-ye37z5h9 7 1 Electron electron NN cord-016755-ye37z5h9 7 2 microscopic microscopic JJ cord-016755-ye37z5h9 7 3 analysis analysis NN cord-016755-ye37z5h9 7 4 revealed reveal VBD cord-016755-ye37z5h9 7 5 a a DT cord-016755-ye37z5h9 7 6 virion virion NN cord-016755-ye37z5h9 7 7 morphologically morphologically RB cord-016755-ye37z5h9 7 8 characteristic characteristic JJ cord-016755-ye37z5h9 7 9 of of IN cord-016755-ye37z5h9 7 10 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 7 11 . . . cord-016755-ye37z5h9 8 1 The the DT cord-016755-ye37z5h9 8 2 virus virus NN cord-016755-ye37z5h9 8 3 was be VBD cord-016755-ye37z5h9 8 4 named name VBN cord-016755-ye37z5h9 8 5 as as IN cord-016755-ye37z5h9 8 6 SFTS SFTS NNP cord-016755-ye37z5h9 8 7 virus virus NN cord-016755-ye37z5h9 8 8 . . . cord-016755-ye37z5h9 9 1 The the DT cord-016755-ye37z5h9 9 2 viral viral JJ cord-016755-ye37z5h9 9 3 RNA RNA NNP cord-016755-ye37z5h9 9 4 and/or and/or CC cord-016755-ye37z5h9 9 5 specific specific JJ cord-016755-ye37z5h9 9 6 antibodies antibody NNS cord-016755-ye37z5h9 9 7 were be VBD cord-016755-ye37z5h9 9 8 detected detect VBN cord-016755-ye37z5h9 9 9 from from IN cord-016755-ye37z5h9 9 10 the the DT cord-016755-ye37z5h9 9 11 blood blood NN cord-016755-ye37z5h9 9 12 of of IN cord-016755-ye37z5h9 9 13 patients patient NNS cord-016755-ye37z5h9 9 14 with with IN cord-016755-ye37z5h9 9 15 SFTS SFTS NNP cord-016755-ye37z5h9 9 16 . . . cord-016755-ye37z5h9 10 1 Serological serological JJ cord-016755-ye37z5h9 10 2 assays assay NNS cord-016755-ye37z5h9 10 3 demonstrated demonstrate VBD cord-016755-ye37z5h9 10 4 a a DT cord-016755-ye37z5h9 10 5 virus virus NN cord-016755-ye37z5h9 10 6 - - HYPH cord-016755-ye37z5h9 10 7 specific specific JJ cord-016755-ye37z5h9 10 8 immune immune JJ cord-016755-ye37z5h9 10 9 response response NN cord-016755-ye37z5h9 10 10 in in IN cord-016755-ye37z5h9 10 11 pairs pair NNS cord-016755-ye37z5h9 10 12 of of IN cord-016755-ye37z5h9 10 13 sera sera NNP cord-016755-ye37z5h9 10 14 collected collect VBN cord-016755-ye37z5h9 10 15 from from IN cord-016755-ye37z5h9 10 16 patients patient NNS cord-016755-ye37z5h9 10 17 at at IN cord-016755-ye37z5h9 10 18 acute acute JJ cord-016755-ye37z5h9 10 19 and and CC cord-016755-ye37z5h9 10 20 convalescent convalescent JJ cord-016755-ye37z5h9 10 21 phases phase NNS cord-016755-ye37z5h9 10 22 . . . cord-016755-ye37z5h9 11 1 The the DT cord-016755-ye37z5h9 11 2 pathogenic pathogenic JJ cord-016755-ye37z5h9 11 3 mechanisms mechanism NNS cord-016755-ye37z5h9 11 4 of of IN cord-016755-ye37z5h9 11 5 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 11 6 in in IN cord-016755-ye37z5h9 11 7 human human JJ cord-016755-ye37z5h9 11 8 SFTS SFTS NNP cord-016755-ye37z5h9 11 9 disease disease NN cord-016755-ye37z5h9 11 10 was be VBD cord-016755-ye37z5h9 11 11 resembled resemble VBN cord-016755-ye37z5h9 11 12 in in IN cord-016755-ye37z5h9 11 13 a a DT cord-016755-ye37z5h9 11 14 mouse mouse NN cord-016755-ye37z5h9 11 15 model model NN cord-016755-ye37z5h9 11 16 . . . cord-016755-ye37z5h9 12 1 The the DT cord-016755-ye37z5h9 12 2 results result NNS cord-016755-ye37z5h9 12 3 had have VBD cord-016755-ye37z5h9 12 4 been be VBN cord-016755-ye37z5h9 12 5 collected collect VBN cord-016755-ye37z5h9 12 6 to to TO cord-016755-ye37z5h9 12 7 demonstrate demonstrate VB cord-016755-ye37z5h9 12 8 that that IN cord-016755-ye37z5h9 12 9 SFTS SFTS NNP cord-016755-ye37z5h9 12 10 virus virus NN cord-016755-ye37z5h9 12 11 was be VBD cord-016755-ye37z5h9 12 12 etiologically etiologically RB cord-016755-ye37z5h9 12 13 associated associate VBN cord-016755-ye37z5h9 12 14 with with IN cord-016755-ye37z5h9 12 15 an an DT cord-016755-ye37z5h9 12 16 acute acute JJ cord-016755-ye37z5h9 12 17 and and CC cord-016755-ye37z5h9 12 18 novel novel JJ cord-016755-ye37z5h9 12 19 infectious infectious JJ cord-016755-ye37z5h9 12 20 disease disease NN cord-016755-ye37z5h9 12 21 , , , cord-016755-ye37z5h9 12 22 SFTS SFTS NNP cord-016755-ye37z5h9 12 23 in in IN cord-016755-ye37z5h9 12 24 humans human NNS cord-016755-ye37z5h9 12 25 . . . cord-016755-ye37z5h9 13 1 Surveillance surveillance NN cord-016755-ye37z5h9 13 2 of of IN cord-016755-ye37z5h9 13 3 infectious infectious JJ cord-016755-ye37z5h9 13 4 diseases disease NNS cord-016755-ye37z5h9 13 5 in in IN cord-016755-ye37z5h9 13 6 China China NNP cord-016755-ye37z5h9 13 7 has have VBZ cord-016755-ye37z5h9 13 8 been be VBN cord-016755-ye37z5h9 13 9 significantly significantly RB cord-016755-ye37z5h9 13 10 enhanced enhance VBN cord-016755-ye37z5h9 13 11 since since IN cord-016755-ye37z5h9 13 12 2004 2004 CD cord-016755-ye37z5h9 13 13 after after IN cord-016755-ye37z5h9 13 14 the the DT cord-016755-ye37z5h9 13 15 outbreak outbreak NN cord-016755-ye37z5h9 13 16 of of IN cord-016755-ye37z5h9 13 17 severe severe JJ cord-016755-ye37z5h9 13 18 acute acute JJ cord-016755-ye37z5h9 13 19 respiratory respiratory JJ cord-016755-ye37z5h9 13 20 syndrome syndrome NN cord-016755-ye37z5h9 13 21 ( ( -LRB- cord-016755-ye37z5h9 13 22 SARS SARS NNP cord-016755-ye37z5h9 13 23 ) ) -RRB- cord-016755-ye37z5h9 13 24 . . . cord-016755-ye37z5h9 14 1 Over over IN cord-016755-ye37z5h9 14 2 the the DT cord-016755-ye37z5h9 14 3 years year NNS cord-016755-ye37z5h9 14 4 sporadic sporadic JJ cord-016755-ye37z5h9 14 5 cases case NNS cord-016755-ye37z5h9 14 6 of of IN cord-016755-ye37z5h9 14 7 severe severe JJ cord-016755-ye37z5h9 14 8 acute acute JJ cord-016755-ye37z5h9 14 9 febrile febrile JJ cord-016755-ye37z5h9 14 10 illness illness NN cord-016755-ye37z5h9 14 11 with with IN cord-016755-ye37z5h9 14 12 unidentified unidentified JJ cord-016755-ye37z5h9 14 13 cause cause NN cord-016755-ye37z5h9 14 14 were be VBD cord-016755-ye37z5h9 14 15 noted note VBN cord-016755-ye37z5h9 14 16 . . . cord-016755-ye37z5h9 15 1 A a DT cord-016755-ye37z5h9 15 2 unique unique JJ cord-016755-ye37z5h9 15 3 group group NN cord-016755-ye37z5h9 15 4 of of IN cord-016755-ye37z5h9 15 5 hospitalized hospitalize VBN cord-016755-ye37z5h9 15 6 patients patient NNS cord-016755-ye37z5h9 15 7 suffering suffer VBG cord-016755-ye37z5h9 15 8 from from IN cord-016755-ye37z5h9 15 9 acute acute JJ cord-016755-ye37z5h9 15 10 high high JJ cord-016755-ye37z5h9 15 11 fever fever NN cord-016755-ye37z5h9 15 12 accompanied accompany VBN cord-016755-ye37z5h9 15 13 with with IN cord-016755-ye37z5h9 15 14 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 15 15 was be VBD cord-016755-ye37z5h9 15 16 identified identify VBN cord-016755-ye37z5h9 15 17 . . . cord-016755-ye37z5h9 16 1 Although although IN cord-016755-ye37z5h9 16 2 Anaplasma Anaplasma NNP cord-016755-ye37z5h9 16 3 phagocytophilum phagocytophilum NN cord-016755-ye37z5h9 16 4 found find VBD cord-016755-ye37z5h9 16 5 in in IN cord-016755-ye37z5h9 16 6 some some DT cord-016755-ye37z5h9 16 7 of of IN cord-016755-ye37z5h9 16 8 these these DT cord-016755-ye37z5h9 16 9 cases case NNS cord-016755-ye37z5h9 16 10 ( ( -LRB- cord-016755-ye37z5h9 16 11 Zhang Zhang NNP cord-016755-ye37z5h9 16 12 et et NNP cord-016755-ye37z5h9 16 13 al al NNP cord-016755-ye37z5h9 16 14 . . NNP cord-016755-ye37z5h9 16 15 2008 2008 CD cord-016755-ye37z5h9 16 16 ) ) -RRB- cord-016755-ye37z5h9 16 17 , , , cord-016755-ye37z5h9 16 18 which which WDT cord-016755-ye37z5h9 16 19 was be VBD cord-016755-ye37z5h9 16 20 suggested suggest VBN cord-016755-ye37z5h9 16 21 as as IN cord-016755-ye37z5h9 16 22 the the DT cord-016755-ye37z5h9 16 23 cause cause NN cord-016755-ye37z5h9 16 24 of of IN cord-016755-ye37z5h9 16 25 this this DT cord-016755-ye37z5h9 16 26 disease disease NN cord-016755-ye37z5h9 16 27 , , , cord-016755-ye37z5h9 16 28 neither neither CC cord-016755-ye37z5h9 16 29 bacterial bacterial JJ cord-016755-ye37z5h9 16 30 DNA dna NN cord-016755-ye37z5h9 16 31 nor nor CC cord-016755-ye37z5h9 16 32 antibodies antibody NNS cord-016755-ye37z5h9 16 33 against against IN cord-016755-ye37z5h9 16 34 this this DT cord-016755-ye37z5h9 16 35 bacterium bacterium NN cord-016755-ye37z5h9 16 36 could could MD cord-016755-ye37z5h9 16 37 be be VB cord-016755-ye37z5h9 16 38 detected detect VBN cord-016755-ye37z5h9 16 39 in in IN cord-016755-ye37z5h9 16 40 the the DT cord-016755-ye37z5h9 16 41 majority majority NN cord-016755-ye37z5h9 16 42 of of IN cord-016755-ye37z5h9 16 43 patients patient NNS cord-016755-ye37z5h9 16 44 within within IN cord-016755-ye37z5h9 16 45 this this DT cord-016755-ye37z5h9 16 46 group group NN cord-016755-ye37z5h9 16 47 . . . cord-016755-ye37z5h9 17 1 Other other JJ cord-016755-ye37z5h9 17 2 infectious infectious JJ cord-016755-ye37z5h9 17 3 causes cause NNS cord-016755-ye37z5h9 17 4 including include VBG cord-016755-ye37z5h9 17 5 bunyaviruses bunyavirus NNS cord-016755-ye37z5h9 17 6 including include VBG cord-016755-ye37z5h9 17 7 hantavirus hantavirus NN cord-016755-ye37z5h9 17 8 , , , cord-016755-ye37z5h9 17 9 Rift Rift NNP cord-016755-ye37z5h9 17 10 Valley Valley NNP cord-016755-ye37z5h9 17 11 fever fever NN cord-016755-ye37z5h9 17 12 virus virus NN cord-016755-ye37z5h9 17 13 , , , cord-016755-ye37z5h9 17 14 Toscana Toscana NNP cord-016755-ye37z5h9 17 15 virus virus NN cord-016755-ye37z5h9 17 16 , , , cord-016755-ye37z5h9 17 17 and and CC cord-016755-ye37z5h9 17 18 Crimean Crimean NNP cord-016755-ye37z5h9 17 19 - - HYPH cord-016755-ye37z5h9 17 20 Congo Congo NNP cord-016755-ye37z5h9 17 21 hemorrhagic hemorrhagic JJ cord-016755-ye37z5h9 17 22 fever fever NN cord-016755-ye37z5h9 17 23 virus virus NN cord-016755-ye37z5h9 17 24 , , , cord-016755-ye37z5h9 17 25 flaviviruses flavivirus NNS cord-016755-ye37z5h9 17 26 including include VBG cord-016755-ye37z5h9 17 27 dengue dengue NN cord-016755-ye37z5h9 17 28 virus virus NN cord-016755-ye37z5h9 17 29 , , , cord-016755-ye37z5h9 17 30 filovirus filovirus NN cord-016755-ye37z5h9 17 31 such such JJ cord-016755-ye37z5h9 17 32 as as IN cord-016755-ye37z5h9 17 33 Ebola Ebola NNP cord-016755-ye37z5h9 17 34 virus virus NN cord-016755-ye37z5h9 17 35 and and CC cord-016755-ye37z5h9 17 36 Marburg Marburg NNP cord-016755-ye37z5h9 17 37 virus virus NN cord-016755-ye37z5h9 17 38 , , , cord-016755-ye37z5h9 17 39 arenaviruses arenaviruses FW cord-016755-ye37z5h9 17 40 including include VBG cord-016755-ye37z5h9 17 41 Lassa Lassa NNP cord-016755-ye37z5h9 17 42 virus virus NN cord-016755-ye37z5h9 17 43 and and CC cord-016755-ye37z5h9 17 44 Junin Junin NNP cord-016755-ye37z5h9 17 45 virus virus NN cord-016755-ye37z5h9 17 46 , , , cord-016755-ye37z5h9 17 47 alphavirus alphavirus NNP cord-016755-ye37z5h9 17 48 , , , cord-016755-ye37z5h9 17 49 and and CC cord-016755-ye37z5h9 17 50 rickettsia rickettsia NNS cord-016755-ye37z5h9 17 51 were be VBD cord-016755-ye37z5h9 17 52 also also RB cord-016755-ye37z5h9 17 53 excluded exclude VBN cord-016755-ye37z5h9 17 54 by by IN cord-016755-ye37z5h9 17 55 reverse reverse NN cord-016755-ye37z5h9 17 56 transcriptionpolymerase transcriptionpolymerase NNP cord-016755-ye37z5h9 17 57 chain chain NN cord-016755-ye37z5h9 17 58 reaction reaction NN cord-016755-ye37z5h9 17 59 ( ( -LRB- cord-016755-ye37z5h9 17 60 RT RT NNP cord-016755-ye37z5h9 17 61 - - HYPH cord-016755-ye37z5h9 17 62 PCR PCR NNP cord-016755-ye37z5h9 17 63 ) ) -RRB- cord-016755-ye37z5h9 18 1 and/or and/or CC cord-016755-ye37z5h9 18 2 specific specific JJ cord-016755-ye37z5h9 18 3 antibody antibody NN cord-016755-ye37z5h9 18 4 tests test NNS cord-016755-ye37z5h9 18 5 . . . cord-016755-ye37z5h9 19 1 All all DT cord-016755-ye37z5h9 19 2 founded found VBN cord-016755-ye37z5h9 19 3 cases case NNS cord-016755-ye37z5h9 19 4 were be VBD cord-016755-ye37z5h9 19 5 from from IN cord-016755-ye37z5h9 19 6 wooded wooded JJ cord-016755-ye37z5h9 19 7 and and CC cord-016755-ye37z5h9 19 8 hilly hilly JJ cord-016755-ye37z5h9 19 9 upland upland JJ cord-016755-ye37z5h9 19 10 areas area NNS cord-016755-ye37z5h9 19 11 and and CC cord-016755-ye37z5h9 19 12 patients patient NNS cord-016755-ye37z5h9 19 13 were be VBD cord-016755-ye37z5h9 19 14 mostly mostly RB cord-016755-ye37z5h9 19 15 farmers farmer NNS cord-016755-ye37z5h9 19 16 who who WP cord-016755-ye37z5h9 19 17 had have VBD cord-016755-ye37z5h9 19 18 ever ever RB cord-016755-ye37z5h9 19 19 worked work VBN cord-016755-ye37z5h9 19 20 in in IN cord-016755-ye37z5h9 19 21 the the DT cord-016755-ye37z5h9 19 22 field field NN cord-016755-ye37z5h9 19 23 before before IN cord-016755-ye37z5h9 19 24 the the DT cord-016755-ye37z5h9 19 25 onset onset NN cord-016755-ye37z5h9 19 26 of of IN cord-016755-ye37z5h9 19 27 disease disease NN cord-016755-ye37z5h9 19 28 . . . cord-016755-ye37z5h9 20 1 In in IN cord-016755-ye37z5h9 20 2 view view NN cord-016755-ye37z5h9 20 3 of of IN cord-016755-ye37z5h9 20 4 these these DT cord-016755-ye37z5h9 20 5 unusual unusual JJ cord-016755-ye37z5h9 20 6 findings finding NNS cord-016755-ye37z5h9 20 7 , , , cord-016755-ye37z5h9 20 8 an an DT cord-016755-ye37z5h9 20 9 active active JJ cord-016755-ye37z5h9 20 10 surveillance surveillance NN cord-016755-ye37z5h9 20 11 was be VBD cord-016755-ye37z5h9 20 12 implemented implement VBN cord-016755-ye37z5h9 20 13 in in IN cord-016755-ye37z5h9 20 14 several several JJ cord-016755-ye37z5h9 20 15 provinces province NNS cord-016755-ye37z5h9 20 16 in in IN cord-016755-ye37z5h9 20 17 China China NNP cord-016755-ye37z5h9 20 18 since since IN cord-016755-ye37z5h9 20 19 May May NNP cord-016755-ye37z5h9 20 20 2010 2010 CD cord-016755-ye37z5h9 20 21 to to TO cord-016755-ye37z5h9 20 22 identify identify VB cord-016755-ye37z5h9 20 23 patients patient NNS cord-016755-ye37z5h9 20 24 with with IN cord-016755-ye37z5h9 20 25 severe severe JJ cord-016755-ye37z5h9 20 26 fever fever NN cord-016755-ye37z5h9 20 27 with with IN cord-016755-ye37z5h9 20 28 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 20 29 syndrome syndrome NN cord-016755-ye37z5h9 20 30 ( ( -LRB- cord-016755-ye37z5h9 20 31 SFTS SFTS NNP cord-016755-ye37z5h9 20 32 ) ) -RRB- cord-016755-ye37z5h9 20 33 , , , cord-016755-ye37z5h9 20 34 which which WDT cord-016755-ye37z5h9 20 35 is be VBZ cord-016755-ye37z5h9 20 36 characterized characterize VBN cord-016755-ye37z5h9 20 37 by by IN cord-016755-ye37z5h9 20 38 acute acute JJ cord-016755-ye37z5h9 20 39 high high JJ cord-016755-ye37z5h9 20 40 fever fever NN cord-016755-ye37z5h9 20 41 and and CC cord-016755-ye37z5h9 20 42 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 20 43 . . . cord-016755-ye37z5h9 21 1 Collected collect VBN cord-016755-ye37z5h9 21 2 sera sera NNP cord-016755-ye37z5h9 21 3 were be VBD cord-016755-ye37z5h9 21 4 subjected subject VBN cord-016755-ye37z5h9 21 5 to to TO cord-016755-ye37z5h9 21 6 sequence sequence VB cord-016755-ye37z5h9 21 7 independent independent JJ cord-016755-ye37z5h9 21 8 single single JJ cord-016755-ye37z5h9 21 9 primer primer NN cord-016755-ye37z5h9 21 10 amplification amplification NN cord-016755-ye37z5h9 21 11 ( ( -LRB- cord-016755-ye37z5h9 21 12 SISPA SISPA NNP cord-016755-ye37z5h9 21 13 ) ) -RRB- cord-016755-ye37z5h9 21 14 , , , cord-016755-ye37z5h9 21 15 which which WDT cord-016755-ye37z5h9 21 16 provides provide VBZ cord-016755-ye37z5h9 21 17 an an DT cord-016755-ye37z5h9 21 18 opportunity opportunity NN cord-016755-ye37z5h9 21 19 for for IN cord-016755-ye37z5h9 21 20 discovering discover VBG cord-016755-ye37z5h9 21 21 novel novel JJ cord-016755-ye37z5h9 21 22 microbial microbial JJ cord-016755-ye37z5h9 21 23 agents agent NNS cord-016755-ye37z5h9 21 24 directly directly RB cord-016755-ye37z5h9 21 25 from from IN cord-016755-ye37z5h9 21 26 clinical clinical JJ cord-016755-ye37z5h9 21 27 samples sample NNS cord-016755-ye37z5h9 21 28 ( ( -LRB- cord-016755-ye37z5h9 21 29 Allander Allander NNP cord-016755-ye37z5h9 21 30 et et NNP cord-016755-ye37z5h9 21 31 al al NNP cord-016755-ye37z5h9 21 32 . . NNP cord-016755-ye37z5h9 21 33 2001 2001 CD cord-016755-ye37z5h9 21 34 ; ; : cord-016755-ye37z5h9 21 35 Victoria Victoria NNP cord-016755-ye37z5h9 21 36 et et NNP cord-016755-ye37z5h9 21 37 al al NNP cord-016755-ye37z5h9 21 38 . . NNP cord-016755-ye37z5h9 21 39 2008 2008 CD cord-016755-ye37z5h9 21 40 ; ; . cord-016755-ye37z5h9 22 1 Ambrose Ambrose NNP cord-016755-ye37z5h9 22 2 and and CC cord-016755-ye37z5h9 22 3 Clewley Clewley NNP cord-016755-ye37z5h9 22 4 2006 2006 CD cord-016755-ye37z5h9 22 5 ; ; : cord-016755-ye37z5h9 22 6 Jones Jones NNP cord-016755-ye37z5h9 22 7 et et NNP cord-016755-ye37z5h9 22 8 al al NNP cord-016755-ye37z5h9 22 9 . . NNP cord-016755-ye37z5h9 22 10 2005 2005 CD cord-016755-ye37z5h9 22 11 ) ) -RRB- cord-016755-ye37z5h9 22 12 . . . cord-016755-ye37z5h9 23 1 A a DT cord-016755-ye37z5h9 23 2 novel novel NN cord-016755-ye37z5h9 23 3 phlebovirus phlebovirus NN cord-016755-ye37z5h9 23 4 was be VBD cord-016755-ye37z5h9 23 5 identified identify VBN cord-016755-ye37z5h9 23 6 and and CC cord-016755-ye37z5h9 23 7 subsequently subsequently RB cord-016755-ye37z5h9 23 8 isolated isolate VBN cord-016755-ye37z5h9 23 9 in in IN cord-016755-ye37z5h9 23 10 cell cell NN cord-016755-ye37z5h9 23 11 culture culture NN cord-016755-ye37z5h9 23 12 . . . cord-016755-ye37z5h9 24 1 The the DT cord-016755-ye37z5h9 24 2 complete complete JJ cord-016755-ye37z5h9 24 3 genome genome NN cord-016755-ye37z5h9 24 4 of of IN cord-016755-ye37z5h9 24 5 the the DT cord-016755-ye37z5h9 24 6 novel novel JJ cord-016755-ye37z5h9 24 7 virus virus NN cord-016755-ye37z5h9 24 8 was be VBD cord-016755-ye37z5h9 24 9 sequenced sequence VBN cord-016755-ye37z5h9 24 10 . . . cord-016755-ye37z5h9 25 1 Molecular molecular JJ cord-016755-ye37z5h9 25 2 and and CC cord-016755-ye37z5h9 25 3 serological serological JJ cord-016755-ye37z5h9 25 4 assays assay NNS cord-016755-ye37z5h9 25 5 were be VBD cord-016755-ye37z5h9 25 6 performed perform VBN cord-016755-ye37z5h9 25 7 to to TO cord-016755-ye37z5h9 25 8 detect detect VB cord-016755-ye37z5h9 25 9 the the DT cord-016755-ye37z5h9 25 10 virus virus NN cord-016755-ye37z5h9 25 11 in in IN cord-016755-ye37z5h9 25 12 a a DT cord-016755-ye37z5h9 25 13 larger large JJR cord-016755-ye37z5h9 25 14 cohort cohort NN cord-016755-ye37z5h9 25 15 of of IN cord-016755-ye37z5h9 25 16 patients patient NNS cord-016755-ye37z5h9 25 17 with with IN cord-016755-ye37z5h9 25 18 SFTS SFTS NNP cord-016755-ye37z5h9 25 19 . . . cord-016755-ye37z5h9 26 1 The the DT cord-016755-ye37z5h9 26 2 related related JJ cord-016755-ye37z5h9 26 3 data datum NNS cord-016755-ye37z5h9 26 4 had have VBD cord-016755-ye37z5h9 26 5 been be VBN cord-016755-ye37z5h9 26 6 published publish VBN cord-016755-ye37z5h9 27 1 ( ( -LRB- cord-016755-ye37z5h9 27 2 Yu Yu NNP cord-016755-ye37z5h9 27 3 et et NNP cord-016755-ye37z5h9 27 4 al al NNP cord-016755-ye37z5h9 27 5 . . NNP cord-016755-ye37z5h9 27 6 2011 2011 CD cord-016755-ye37z5h9 27 7 ) ) -RRB- cord-016755-ye37z5h9 27 8 . . . cord-016755-ye37z5h9 28 1 Here here RB cord-016755-ye37z5h9 28 2 we -PRON- PRP cord-016755-ye37z5h9 28 3 described describe VBD cord-016755-ye37z5h9 28 4 the the DT cord-016755-ye37z5h9 28 5 process process NN cord-016755-ye37z5h9 28 6 of of IN cord-016755-ye37z5h9 28 7 the the DT cord-016755-ye37z5h9 28 8 discovery discovery NN cord-016755-ye37z5h9 28 9 of of IN cord-016755-ye37z5h9 28 10 SFTS SFTS NNP cord-016755-ye37z5h9 28 11 virus virus NN cord-016755-ye37z5h9 28 12 in in IN cord-016755-ye37z5h9 28 13 China China NNP cord-016755-ye37z5h9 28 14 . . . cord-016755-ye37z5h9 29 1 An an DT cord-016755-ye37z5h9 29 2 active active JJ cord-016755-ye37z5h9 29 3 surveillance surveillance NN cord-016755-ye37z5h9 29 4 scheme scheme NN cord-016755-ye37z5h9 29 5 was be VBD cord-016755-ye37z5h9 29 6 implemented implement VBN cord-016755-ye37z5h9 29 7 in in IN cord-016755-ye37z5h9 29 8 selected select VBN cord-016755-ye37z5h9 29 9 areas area NNS cord-016755-ye37z5h9 29 10 in in IN cord-016755-ye37z5h9 29 11 Hubei Hubei NNP cord-016755-ye37z5h9 29 12 , , , cord-016755-ye37z5h9 29 13 Henan Henan NNP cord-016755-ye37z5h9 29 14 provinces province NNS cord-016755-ye37z5h9 29 15 since since IN cord-016755-ye37z5h9 29 16 May May NNP cord-016755-ye37z5h9 29 17 2010 2010 CD cord-016755-ye37z5h9 29 18 to to TO cord-016755-ye37z5h9 29 19 identify identify VB cord-016755-ye37z5h9 29 20 hospitalized hospitalize VBN cord-016755-ye37z5h9 29 21 patients patient NNS cord-016755-ye37z5h9 29 22 , , , cord-016755-ye37z5h9 29 23 who who WP cord-016755-ye37z5h9 29 24 presented present VBD cord-016755-ye37z5h9 29 25 with with IN cord-016755-ye37z5h9 29 26 an an DT cord-016755-ye37z5h9 29 27 acute acute JJ cord-016755-ye37z5h9 29 28 fever fever NN cord-016755-ye37z5h9 29 29 of of IN cord-016755-ye37z5h9 29 30 ≥38 ≥38 NNP cord-016755-ye37z5h9 29 31 ° ° NN cord-016755-ye37z5h9 29 32 C c NN cord-016755-ye37z5h9 29 33 and and CC cord-016755-ye37z5h9 29 34 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 29 35 with with IN cord-016755-ye37z5h9 29 36 unknown unknown JJ cord-016755-ye37z5h9 29 37 causes cause NNS cord-016755-ye37z5h9 29 38 ( ( -LRB- cord-016755-ye37z5h9 29 39 National national JJ cord-016755-ye37z5h9 29 40 2010 2010 CD cord-016755-ye37z5h9 29 41 ) ) -RRB- cord-016755-ye37z5h9 29 42 . . . cord-016755-ye37z5h9 30 1 Serum serum NN cord-016755-ye37z5h9 30 2 samples sample NNS cord-016755-ye37z5h9 30 3 were be VBD cord-016755-ye37z5h9 30 4 collected collect VBN cord-016755-ye37z5h9 30 5 preferably preferably RB cord-016755-ye37z5h9 30 6 within within IN cord-016755-ye37z5h9 30 7 2 2 CD cord-016755-ye37z5h9 30 8 weeks week NNS cord-016755-ye37z5h9 30 9 after after IN cord-016755-ye37z5h9 30 10 the the DT cord-016755-ye37z5h9 30 11 onset onset NN cord-016755-ye37z5h9 30 12 of of IN cord-016755-ye37z5h9 30 13 fever fever NN cord-016755-ye37z5h9 30 14 and and CC cord-016755-ye37z5h9 30 15 during during IN cord-016755-ye37z5h9 30 16 the the DT cord-016755-ye37z5h9 30 17 convalescent convalescent JJ cord-016755-ye37z5h9 30 18 phase phase NN cord-016755-ye37z5h9 30 19 from from IN cord-016755-ye37z5h9 30 20 six six CD cord-016755-ye37z5h9 30 21 provinces province NNS cord-016755-ye37z5h9 30 22 of of IN cord-016755-ye37z5h9 30 23 Hubei Hubei NNP cord-016755-ye37z5h9 30 24 , , , cord-016755-ye37z5h9 30 25 Henan Henan NNP cord-016755-ye37z5h9 30 26 , , , cord-016755-ye37z5h9 30 27 Shandong Shandong NNP cord-016755-ye37z5h9 30 28 , , , cord-016755-ye37z5h9 30 29 Jiangsu Jiangsu NNP cord-016755-ye37z5h9 30 30 , , , cord-016755-ye37z5h9 30 31 Anhui Anhui NNP cord-016755-ye37z5h9 30 32 and and CC cord-016755-ye37z5h9 30 33 Liaoning Liaoning NNP cord-016755-ye37z5h9 30 34 . . . cord-016755-ye37z5h9 31 1 The the DT cord-016755-ye37z5h9 31 2 cases case NNS cord-016755-ye37z5h9 31 3 of of IN cord-016755-ye37z5h9 31 4 SFTS SFTS NNP cord-016755-ye37z5h9 31 5 occurring occur VBG cord-016755-ye37z5h9 31 6 before before IN cord-016755-ye37z5h9 31 7 May May NNP cord-016755-ye37z5h9 31 8 2010 2010 CD cord-016755-ye37z5h9 31 9 were be VBD cord-016755-ye37z5h9 31 10 also also RB cord-016755-ye37z5h9 31 11 identified identify VBN cord-016755-ye37z5h9 31 12 through through IN cord-016755-ye37z5h9 31 13 retrospective retrospective JJ cord-016755-ye37z5h9 31 14 review review NN cord-016755-ye37z5h9 31 15 , , , cord-016755-ye37z5h9 31 16 and and CC cord-016755-ye37z5h9 31 17 sera sera NNP cord-016755-ye37z5h9 31 18 collected collect VBD cord-016755-ye37z5h9 31 19 were be VBD cord-016755-ye37z5h9 31 20 requested request VBN cord-016755-ye37z5h9 31 21 from from IN cord-016755-ye37z5h9 31 22 the the DT cord-016755-ye37z5h9 31 23 hospitals hospital NNS cord-016755-ye37z5h9 31 24 . . . cord-016755-ye37z5h9 32 1 Cases case NNS cord-016755-ye37z5h9 32 2 fit fit VBP cord-016755-ye37z5h9 32 3 the the DT cord-016755-ye37z5h9 32 4 criteria criterion NNS cord-016755-ye37z5h9 32 5 but but CC cord-016755-ye37z5h9 32 6 with with IN cord-016755-ye37z5h9 32 7 clinical clinical JJ cord-016755-ye37z5h9 32 8 or or CC cord-016755-ye37z5h9 32 9 laboratory laboratory NN cord-016755-ye37z5h9 32 10 confirmed confirm VBD cord-016755-ye37z5h9 32 11 diagnosis diagnosis NN cord-016755-ye37z5h9 32 12 were be VBD cord-016755-ye37z5h9 32 13 excluded exclude VBN cord-016755-ye37z5h9 32 14 . . . cord-016755-ye37z5h9 33 1 The the DT cord-016755-ye37z5h9 33 2 sera sera NNP cord-016755-ye37z5h9 33 3 from from IN cord-016755-ye37z5h9 33 4 200 200 CD cord-016755-ye37z5h9 33 5 patient patient NN cord-016755-ye37z5h9 33 6 - - HYPH cord-016755-ye37z5h9 33 7 matched match VBN cord-016755-ye37z5h9 33 8 healthy healthy JJ cord-016755-ye37z5h9 33 9 donors donor NNS cord-016755-ye37z5h9 33 10 living live VBG cord-016755-ye37z5h9 33 11 in in IN cord-016755-ye37z5h9 33 12 the the DT cord-016755-ye37z5h9 33 13 same same JJ cord-016755-ye37z5h9 33 14 areas area NNS cord-016755-ye37z5h9 33 15 were be VBD cord-016755-ye37z5h9 33 16 also also RB cord-016755-ye37z5h9 33 17 collected collect VBN cord-016755-ye37z5h9 33 18 . . . cord-016755-ye37z5h9 34 1 The the DT cord-016755-ye37z5h9 34 2 research research NN cord-016755-ye37z5h9 34 3 protocol protocol NN cord-016755-ye37z5h9 34 4 was be VBD cord-016755-ye37z5h9 34 5 approved approve VBN cord-016755-ye37z5h9 34 6 by by IN cord-016755-ye37z5h9 34 7 the the DT cord-016755-ye37z5h9 34 8 human human JJ cord-016755-ye37z5h9 34 9 bio bio NNP cord-016755-ye37z5h9 34 10 - - HYPH cord-016755-ye37z5h9 34 11 ethics ethics NNP cord-016755-ye37z5h9 34 12 committee committee NNP cord-016755-ye37z5h9 34 13 of of IN cord-016755-ye37z5h9 34 14 the the DT cord-016755-ye37z5h9 34 15 Chinese chinese JJ cord-016755-ye37z5h9 34 16 CDC CDC NNP cord-016755-ye37z5h9 34 17 , , , cord-016755-ye37z5h9 34 18 and and CC cord-016755-ye37z5h9 34 19 all all DT cord-016755-ye37z5h9 34 20 human human JJ cord-016755-ye37z5h9 34 21 participants participant NNS cord-016755-ye37z5h9 34 22 gave give VBD cord-016755-ye37z5h9 34 23 written write VBN cord-016755-ye37z5h9 34 24 informed informed JJ cord-016755-ye37z5h9 34 25 consent consent NN cord-016755-ye37z5h9 34 26 . . . cord-016755-ye37z5h9 35 1 The the DT cord-016755-ye37z5h9 35 2 amplification amplification NN cord-016755-ye37z5h9 35 3 of of IN cord-016755-ye37z5h9 35 4 microbial microbial JJ cord-016755-ye37z5h9 35 5 nucleic nucleic JJ cord-016755-ye37z5h9 35 6 acids acid NNS cord-016755-ye37z5h9 35 7 from from IN cord-016755-ye37z5h9 35 8 serum serum NN cord-016755-ye37z5h9 35 9 was be VBD cord-016755-ye37z5h9 35 10 based base VBN cord-016755-ye37z5h9 35 11 on on IN cord-016755-ye37z5h9 35 12 a a DT cord-016755-ye37z5h9 35 13 modified modify VBN cord-016755-ye37z5h9 35 14 version version NN cord-016755-ye37z5h9 35 15 of of IN cord-016755-ye37z5h9 35 16 the the DT cord-016755-ye37z5h9 35 17 SISPA SISPA NNP cord-016755-ye37z5h9 35 18 method method NN cord-016755-ye37z5h9 35 19 ( ( -LRB- cord-016755-ye37z5h9 35 20 Allander Allander NNP cord-016755-ye37z5h9 35 21 et et NNP cord-016755-ye37z5h9 35 22 al al NNP cord-016755-ye37z5h9 35 23 . . NNP cord-016755-ye37z5h9 35 24 2001 2001 CD cord-016755-ye37z5h9 35 25 ; ; : cord-016755-ye37z5h9 35 26 Ambrose Ambrose NNP cord-016755-ye37z5h9 35 27 and and CC cord-016755-ye37z5h9 35 28 Clewley Clewley NNP cord-016755-ye37z5h9 35 29 2006 2006 CD cord-016755-ye37z5h9 35 30 ; ; : cord-016755-ye37z5h9 35 31 Jones Jones NNP cord-016755-ye37z5h9 35 32 et et NNP cord-016755-ye37z5h9 35 33 al al NNP cord-016755-ye37z5h9 35 34 . . NNP cord-016755-ye37z5h9 35 35 2005 2005 CD cord-016755-ye37z5h9 35 36 ) ) -RRB- cord-016755-ye37z5h9 35 37 . . . cord-016755-ye37z5h9 36 1 After after IN cord-016755-ye37z5h9 36 2 filtration filtration NN cord-016755-ye37z5h9 36 3 through through IN cord-016755-ye37z5h9 36 4 a a DT cord-016755-ye37z5h9 36 5 0.2-μm 0.2-μm CD cord-016755-ye37z5h9 36 6 filter filter NN cord-016755-ye37z5h9 36 7 and and CC cord-016755-ye37z5h9 36 8 digestion digestion NN cord-016755-ye37z5h9 36 9 with with IN cord-016755-ye37z5h9 36 10 turbo turbo NN cord-016755-ye37z5h9 36 11 DNase DNase NNP cord-016755-ye37z5h9 36 12 ( ( -LRB- cord-016755-ye37z5h9 36 13 Ambion Ambion NNP cord-016755-ye37z5h9 36 14 ) ) -RRB- cord-016755-ye37z5h9 36 15 , , , cord-016755-ye37z5h9 36 16 benzonase benzonase NNP cord-016755-ye37z5h9 36 17 ( ( -LRB- cord-016755-ye37z5h9 36 18 Novagen Novagen NNP cord-016755-ye37z5h9 36 19 ) ) -RRB- cord-016755-ye37z5h9 36 20 and and CC cord-016755-ye37z5h9 36 21 RNase RNase NNP cord-016755-ye37z5h9 36 22 One One NNP cord-016755-ye37z5h9 36 23 ( ( -LRB- cord-016755-ye37z5h9 36 24 Promega Promega NNP cord-016755-ye37z5h9 36 25 ) ) -RRB- cord-016755-ye37z5h9 36 26 , , , cord-016755-ye37z5h9 36 27 RNA RNA NNP cord-016755-ye37z5h9 36 28 was be VBD cord-016755-ye37z5h9 36 29 extracted extract VBN cord-016755-ye37z5h9 36 30 from from IN cord-016755-ye37z5h9 36 31 serum serum NN cord-016755-ye37z5h9 36 32 ( ( -LRB- cord-016755-ye37z5h9 36 33 140 140 CD cord-016755-ye37z5h9 36 34 μL μL NNP cord-016755-ye37z5h9 36 35 ) ) -RRB- cord-016755-ye37z5h9 36 36 of of IN cord-016755-ye37z5h9 36 37 patient patient JJ cord-016755-ye37z5h9 36 38 HB29 HB29 NNP cord-016755-ye37z5h9 36 39 . . . cord-016755-ye37z5h9 37 1 RNA RNA NNP cord-016755-ye37z5h9 37 2 preparations preparation NNS cord-016755-ye37z5h9 37 3 were be VBD cord-016755-ye37z5h9 37 4 reverse reverse VB cord-016755-ye37z5h9 37 5 transcribed transcribe VBN cord-016755-ye37z5h9 37 6 into into IN cord-016755-ye37z5h9 37 7 cDNA cdna NN cord-016755-ye37z5h9 37 8 and and CC cord-016755-ye37z5h9 37 9 second second JJ cord-016755-ye37z5h9 37 10 strand strand NN cord-016755-ye37z5h9 37 11 cDNA cdna NN cord-016755-ye37z5h9 37 12 were be VBD cord-016755-ye37z5h9 37 13 synthesized synthesize VBN cord-016755-ye37z5h9 37 14 . . . cord-016755-ye37z5h9 38 1 After after IN cord-016755-ye37z5h9 38 2 purification purification NN cord-016755-ye37z5h9 38 3 , , , cord-016755-ye37z5h9 38 4 the the DT cord-016755-ye37z5h9 38 5 DNA dna NN cord-016755-ye37z5h9 38 6 was be VBD cord-016755-ye37z5h9 38 7 ligated ligate VBN cord-016755-ye37z5h9 38 8 to to IN cord-016755-ye37z5h9 38 9 a a DT cord-016755-ye37z5h9 38 10 phosphorylated phosphorylated JJ cord-016755-ye37z5h9 38 11 blunt blunt JJ cord-016755-ye37z5h9 38 12 adapter adapter NN cord-016755-ye37z5h9 38 13 E19 E19 NNP cord-016755-ye37z5h9 38 14 ( ( -LRB- cord-016755-ye37z5h9 38 15 5′-AGCAATTCCGTTGCTGTCG-3′ 5′-agcaattccgttgctgtcg-3′ CD cord-016755-ye37z5h9 38 16 ) ) -RRB- cord-016755-ye37z5h9 38 17 ; ; : cord-016755-ye37z5h9 38 18 and and CC cord-016755-ye37z5h9 38 19 E12 E12 NNP cord-016755-ye37z5h9 38 20 ( ( -LRB- cord-016755-ye37z5h9 38 21 5′-P 5′-p CD cord-016755-ye37z5h9 38 22 - - HYPH cord-016755-ye37z5h9 38 23 GGCAACGACAGC-3′ GGCAACGACAGC-3′ NNP cord-016755-ye37z5h9 38 24 ) ) -RRB- cord-016755-ye37z5h9 38 25 . . . cord-016755-ye37z5h9 39 1 The the DT cord-016755-ye37z5h9 39 2 ligation ligation NN cord-016755-ye37z5h9 39 3 product product NN cord-016755-ye37z5h9 39 4 was be VBD cord-016755-ye37z5h9 39 5 PCR PCR NNP cord-016755-ye37z5h9 39 6 amplified amplify VBN cord-016755-ye37z5h9 39 7 and and CC cord-016755-ye37z5h9 39 8 separated separate VBN cord-016755-ye37z5h9 39 9 by by IN cord-016755-ye37z5h9 39 10 agarose agarose JJ cord-016755-ye37z5h9 39 11 gel gel NN cord-016755-ye37z5h9 39 12 electrophoresis electrophoresis NN cord-016755-ye37z5h9 39 13 . . . cord-016755-ye37z5h9 40 1 Fragments fragment NNS cord-016755-ye37z5h9 40 2 of of IN cord-016755-ye37z5h9 40 3 different different JJ cord-016755-ye37z5h9 40 4 size size NN cord-016755-ye37z5h9 40 5 were be VBD cord-016755-ye37z5h9 40 6 isolated isolate VBN cord-016755-ye37z5h9 40 7 and and CC cord-016755-ye37z5h9 40 8 cloned clone VBN cord-016755-ye37z5h9 40 9 . . . cord-016755-ye37z5h9 41 1 A a DT cord-016755-ye37z5h9 41 2 total total NN cord-016755-ye37z5h9 41 3 of of IN cord-016755-ye37z5h9 41 4 576 576 CD cord-016755-ye37z5h9 41 5 cDNA cdna NN cord-016755-ye37z5h9 41 6 clones clone NNS cord-016755-ye37z5h9 41 7 were be VBD cord-016755-ye37z5h9 41 8 picked pick VBN cord-016755-ye37z5h9 41 9 by by IN cord-016755-ye37z5h9 41 10 SISPA SISPA NNP cord-016755-ye37z5h9 41 11 from from IN cord-016755-ye37z5h9 41 12 the the DT cord-016755-ye37z5h9 41 13 serum serum NN cord-016755-ye37z5h9 41 14 of of IN cord-016755-ye37z5h9 41 15 patient patient NN cord-016755-ye37z5h9 41 16 HB29 HB29 NNP cord-016755-ye37z5h9 41 17 and and CC cord-016755-ye37z5h9 41 18 sequenced sequence VBN cord-016755-ye37z5h9 41 19 . . . cord-016755-ye37z5h9 42 1 After after IN cord-016755-ye37z5h9 42 2 trimming trim VBG cord-016755-ye37z5h9 42 3 to to TO cord-016755-ye37z5h9 42 4 remove remove VB cord-016755-ye37z5h9 42 5 sequences sequence NNS cord-016755-ye37z5h9 42 6 derived derive VBN cord-016755-ye37z5h9 42 7 from from IN cord-016755-ye37z5h9 42 8 the the DT cord-016755-ye37z5h9 42 9 amplification amplification NN cord-016755-ye37z5h9 42 10 primer primer NN cord-016755-ye37z5h9 42 11 , , , cord-016755-ye37z5h9 42 12 the the DT cord-016755-ye37z5h9 42 13 data datum NNS cord-016755-ye37z5h9 42 14 set set NN cord-016755-ye37z5h9 42 15 was be VBD cord-016755-ye37z5h9 42 16 subjected subject VBN cord-016755-ye37z5h9 42 17 to to IN cord-016755-ye37z5h9 42 18 homology homology NN cord-016755-ye37z5h9 42 19 search search NN cord-016755-ye37z5h9 42 20 with with IN cord-016755-ye37z5h9 42 21 the the DT cord-016755-ye37z5h9 42 22 GenBank GenBank NNP cord-016755-ye37z5h9 42 23 databases database NNS cord-016755-ye37z5h9 42 24 of of IN cord-016755-ye37z5h9 42 25 nucleic nucleic JJ cord-016755-ye37z5h9 42 26 acids acid NNS cord-016755-ye37z5h9 42 27 and and CC cord-016755-ye37z5h9 42 28 proteins protein NNS cord-016755-ye37z5h9 42 29 using use VBG cord-016755-ye37z5h9 42 30 BLASTN BLASTN NNP cord-016755-ye37z5h9 42 31 and and CC cord-016755-ye37z5h9 42 32 BLASTX BLASTX NNP cord-016755-ye37z5h9 42 33 . . . cord-016755-ye37z5h9 43 1 Whereas whereas IN cord-016755-ye37z5h9 43 2 the the DT cord-016755-ye37z5h9 43 3 nucleotide nucleotide JJ cord-016755-ye37z5h9 43 4 sequence sequence NN cord-016755-ye37z5h9 43 5 was be VBD cord-016755-ye37z5h9 43 6 essentially essentially RB cord-016755-ye37z5h9 43 7 unrelated unrelated JJ cord-016755-ye37z5h9 43 8 to to IN cord-016755-ye37z5h9 43 9 other other JJ cord-016755-ye37z5h9 43 10 sequences sequence NNS cord-016755-ye37z5h9 43 11 in in IN cord-016755-ye37z5h9 43 12 the the DT cord-016755-ye37z5h9 43 13 existing exist VBG cord-016755-ye37z5h9 43 14 GeneBank GeneBank NNP cord-016755-ye37z5h9 43 15 database database NN cord-016755-ye37z5h9 43 16 ( ( -LRB- cord-016755-ye37z5h9 43 17 http:// http:// NNP cord-016755-ye37z5h9 43 18 blast.ncbi.nlm.gov blast.ncbi.nlm.gov NNP cord-016755-ye37z5h9 43 19 ) ) -RRB- cord-016755-ye37z5h9 43 20 , , , cord-016755-ye37z5h9 43 21 the the DT cord-016755-ye37z5h9 43 22 deduced deduce VBN cord-016755-ye37z5h9 43 23 amino amino NN cord-016755-ye37z5h9 43 24 acid acid NN cord-016755-ye37z5h9 43 25 sequence sequence NN cord-016755-ye37z5h9 43 26 from from IN cord-016755-ye37z5h9 43 27 14 14 CD cord-016755-ye37z5h9 43 28 cDNA cdna NN cord-016755-ye37z5h9 43 29 clones clone NNS cord-016755-ye37z5h9 43 30 showed show VBD cord-016755-ye37z5h9 43 31 20 20 CD cord-016755-ye37z5h9 43 32 - - SYM cord-016755-ye37z5h9 43 33 30 30 CD cord-016755-ye37z5h9 43 34 % % NN cord-016755-ye37z5h9 43 35 homology homology NN cord-016755-ye37z5h9 43 36 to to IN cord-016755-ye37z5h9 43 37 viral viral JJ cord-016755-ye37z5h9 43 38 proteins protein NNS cord-016755-ye37z5h9 43 39 , , , cord-016755-ye37z5h9 43 40 including include VBG cord-016755-ye37z5h9 43 41 RNA RNA NNP cord-016755-ye37z5h9 43 42 dependent dependent JJ cord-016755-ye37z5h9 43 43 RNA RNA NNP cord-016755-ye37z5h9 43 44 polymerase polymerase NN cord-016755-ye37z5h9 43 45 ( ( -LRB- cord-016755-ye37z5h9 43 46 RdRP RdRP NNP cord-016755-ye37z5h9 43 47 ) ) -RRB- cord-016755-ye37z5h9 43 48 , , , cord-016755-ye37z5h9 43 49 membrane membrane NN cord-016755-ye37z5h9 43 50 polyprotein polyprotein NN cord-016755-ye37z5h9 43 51 and and CC cord-016755-ye37z5h9 43 52 nonstructural nonstructural JJ cord-016755-ye37z5h9 43 53 S S NNP cord-016755-ye37z5h9 43 54 protein protein NN cord-016755-ye37z5h9 43 55 , , , cord-016755-ye37z5h9 43 56 of of IN cord-016755-ye37z5h9 43 57 known know VBN cord-016755-ye37z5h9 43 58 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 43 59 such such JJ cord-016755-ye37z5h9 43 60 as as IN cord-016755-ye37z5h9 43 61 Rift Rift NNP cord-016755-ye37z5h9 43 62 Valley Valley NNP cord-016755-ye37z5h9 43 63 fever fever NN cord-016755-ye37z5h9 43 64 virus virus NN cord-016755-ye37z5h9 43 65 and and CC cord-016755-ye37z5h9 43 66 Uukuniemi Uukuniemi NNP cord-016755-ye37z5h9 43 67 virus virus NN cord-016755-ye37z5h9 43 68 . . . cord-016755-ye37z5h9 44 1 Walking walk VBG cord-016755-ye37z5h9 44 2 primers primer NNS cord-016755-ye37z5h9 44 3 from from IN cord-016755-ye37z5h9 44 4 both both DT cord-016755-ye37z5h9 44 5 5′ 5′ CD cord-016755-ye37z5h9 44 6 and and CC cord-016755-ye37z5h9 44 7 3′ 3′ CD cord-016755-ye37z5h9 44 8 directions direction NNS cord-016755-ye37z5h9 44 9 were be VBD cord-016755-ye37z5h9 44 10 designed design VBN cord-016755-ye37z5h9 44 11 based base VBN cord-016755-ye37z5h9 44 12 on on IN cord-016755-ye37z5h9 44 13 the the DT cord-016755-ye37z5h9 44 14 sequence sequence NN cord-016755-ye37z5h9 44 15 of of IN cord-016755-ye37z5h9 44 16 gene gene NN cord-016755-ye37z5h9 44 17 fragments fragment NNS cord-016755-ye37z5h9 44 18 obtained obtain VBN cord-016755-ye37z5h9 44 19 from from IN cord-016755-ye37z5h9 44 20 SISPA SISPA NNP cord-016755-ye37z5h9 44 21 . . . cord-016755-ye37z5h9 45 1 The the DT cord-016755-ye37z5h9 45 2 three three CD cord-016755-ye37z5h9 45 3 genomic genomic JJ cord-016755-ye37z5h9 45 4 segments segment NNS cord-016755-ye37z5h9 45 5 were be VBD cord-016755-ye37z5h9 45 6 assembled assemble VBN cord-016755-ye37z5h9 45 7 from from IN cord-016755-ye37z5h9 45 8 a a DT cord-016755-ye37z5h9 45 9 series series NN cord-016755-ye37z5h9 45 10 of of IN cord-016755-ye37z5h9 45 11 overlapping overlap VBG cord-016755-ye37z5h9 45 12 cDNA cdna NN cord-016755-ye37z5h9 45 13 clones clone NNS cord-016755-ye37z5h9 45 14 . . . cord-016755-ye37z5h9 46 1 The the DT cord-016755-ye37z5h9 46 2 5′ 5′ CD cord-016755-ye37z5h9 46 3 and and CC cord-016755-ye37z5h9 46 4 3′ 3′ CD cord-016755-ye37z5h9 46 5 termini terminus NNS cord-016755-ye37z5h9 46 6 of of IN cord-016755-ye37z5h9 46 7 viral viral JJ cord-016755-ye37z5h9 46 8 RNA RNA NNP cord-016755-ye37z5h9 46 9 segments segment NNS cord-016755-ye37z5h9 46 10 were be VBD cord-016755-ye37z5h9 46 11 determined determine VBN cord-016755-ye37z5h9 46 12 by by IN cord-016755-ye37z5h9 46 13 rapid rapid JJ cord-016755-ye37z5h9 46 14 amplification amplification NN cord-016755-ye37z5h9 46 15 of of IN cord-016755-ye37z5h9 46 16 cDNA cdna NN cord-016755-ye37z5h9 46 17 ends end NNS cord-016755-ye37z5h9 46 18 ( ( -LRB- cord-016755-ye37z5h9 46 19 RACE race NN cord-016755-ye37z5h9 46 20 ) ) -RRB- cord-016755-ye37z5h9 46 21 using use VBG cord-016755-ye37z5h9 46 22 reagents reagent NNS cord-016755-ye37z5h9 46 23 purchased purchase VBN cord-016755-ye37z5h9 46 24 from from IN cord-016755-ye37z5h9 46 25 Invitrogen Invitrogen NNP cord-016755-ye37z5h9 46 26 . . . cord-016755-ye37z5h9 47 1 To to TO cord-016755-ye37z5h9 47 2 eliminate eliminate VB cord-016755-ye37z5h9 47 3 the the DT cord-016755-ye37z5h9 47 4 influence influence NN cord-016755-ye37z5h9 47 5 of of IN cord-016755-ye37z5h9 47 6 cloning clone VBG cord-016755-ye37z5h9 47 7 bias bias NN cord-016755-ye37z5h9 47 8 on on IN cord-016755-ye37z5h9 47 9 genome genome NN cord-016755-ye37z5h9 47 10 sequencing sequencing NN cord-016755-ye37z5h9 47 11 , , , cord-016755-ye37z5h9 47 12 direct direct JJ cord-016755-ye37z5h9 47 13 PCR PCR NNP cord-016755-ye37z5h9 47 14 re re NN cord-016755-ye37z5h9 47 15 - - NN cord-016755-ye37z5h9 47 16 sequencing sequencing NN cord-016755-ye37z5h9 47 17 of of IN cord-016755-ye37z5h9 47 18 all all DT cord-016755-ye37z5h9 47 19 three three CD cord-016755-ye37z5h9 47 20 genomic genomic JJ cord-016755-ye37z5h9 47 21 segments segment NNS cord-016755-ye37z5h9 47 22 were be VBD cord-016755-ye37z5h9 47 23 performed perform VBN cord-016755-ye37z5h9 47 24 with with IN cord-016755-ye37z5h9 47 25 newly newly RB cord-016755-ye37z5h9 47 26 designed design VBN cord-016755-ye37z5h9 47 27 primers primer NNS cord-016755-ye37z5h9 47 28 . . . cord-016755-ye37z5h9 48 1 The the DT cord-016755-ye37z5h9 48 2 partial partial JJ cord-016755-ye37z5h9 48 3 sequences sequence NNS cord-016755-ye37z5h9 48 4 were be VBD cord-016755-ye37z5h9 48 5 obtained obtain VBN cord-016755-ye37z5h9 48 6 from from IN cord-016755-ye37z5h9 48 7 the the DT cord-016755-ye37z5h9 48 8 first first JJ cord-016755-ye37z5h9 48 9 isolated isolate VBN cord-016755-ye37z5h9 48 10 virus virus NN cord-016755-ye37z5h9 48 11 strain strain NN cord-016755-ye37z5h9 48 12 DBM DBM NNP cord-016755-ye37z5h9 48 13 , , , cord-016755-ye37z5h9 48 14 and and CC cord-016755-ye37z5h9 48 15 the the DT cord-016755-ye37z5h9 48 16 complete complete JJ cord-016755-ye37z5h9 48 17 genomes genome NNS cord-016755-ye37z5h9 48 18 of of IN cord-016755-ye37z5h9 48 19 11 11 CD cord-016755-ye37z5h9 48 20 isolates isolate NNS cord-016755-ye37z5h9 48 21 of of IN cord-016755-ye37z5h9 48 22 SFTS SFTS NNP cord-016755-ye37z5h9 48 23 virus virus NN cord-016755-ye37z5h9 48 24 were be VBD cord-016755-ye37z5h9 48 25 further further RB cord-016755-ye37z5h9 48 26 sequenced sequence VBN cord-016755-ye37z5h9 49 1 ( ( -LRB- cord-016755-ye37z5h9 49 2 Yu Yu NNP cord-016755-ye37z5h9 49 3 et et NNP cord-016755-ye37z5h9 49 4 al al NNP cord-016755-ye37z5h9 49 5 . . NNP cord-016755-ye37z5h9 49 6 2011 2011 CD cord-016755-ye37z5h9 49 7 ) ) -RRB- cord-016755-ye37z5h9 49 8 . . . cord-016755-ye37z5h9 50 1 All all DT cord-016755-ye37z5h9 50 2 isolates isolate NNS cord-016755-ye37z5h9 50 3 including include VBG cord-016755-ye37z5h9 50 4 strain strain NN cord-016755-ye37z5h9 50 5 DBM DBM NNP cord-016755-ye37z5h9 50 6 were be VBD cord-016755-ye37z5h9 50 7 closely closely RB cord-016755-ye37z5h9 50 8 related relate VBN cord-016755-ye37z5h9 51 1 ( ( -LRB- cord-016755-ye37z5h9 51 2 Yu Yu NNP cord-016755-ye37z5h9 51 3 et et NNP cord-016755-ye37z5h9 51 4 al al NNP cord-016755-ye37z5h9 51 5 . . NNP cord-016755-ye37z5h9 51 6 2011 2011 CD cord-016755-ye37z5h9 51 7 ) ) -RRB- cord-016755-ye37z5h9 51 8 . . . cord-016755-ye37z5h9 52 1 Termini Termini NNP cord-016755-ye37z5h9 52 2 of of IN cord-016755-ye37z5h9 52 3 three three CD cord-016755-ye37z5h9 52 4 genomic genomic JJ cord-016755-ye37z5h9 52 5 segments segment NNS cord-016755-ye37z5h9 52 6 of of IN cord-016755-ye37z5h9 52 7 SFTS SFTS NNP cord-016755-ye37z5h9 52 8 virus virus NN cord-016755-ye37z5h9 52 9 were be VBD cord-016755-ye37z5h9 52 10 found find VBN cord-016755-ye37z5h9 52 11 to to TO cord-016755-ye37z5h9 52 12 be be VB cord-016755-ye37z5h9 52 13 similar similar JJ cord-016755-ye37z5h9 52 14 to to IN cord-016755-ye37z5h9 52 15 counterparts counterpart NNS cord-016755-ye37z5h9 52 16 in in IN cord-016755-ye37z5h9 52 17 other other JJ cord-016755-ye37z5h9 52 18 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 52 19 . . . cord-016755-ye37z5h9 53 1 Phylogenetic phylogenetic JJ cord-016755-ye37z5h9 53 2 analyses analysis NNS cord-016755-ye37z5h9 53 3 were be VBD cord-016755-ye37z5h9 53 4 performed perform VBN cord-016755-ye37z5h9 53 5 with with IN cord-016755-ye37z5h9 53 6 the the DT cord-016755-ye37z5h9 53 7 neighbor neighbor NN cord-016755-ye37z5h9 53 8 - - HYPH cord-016755-ye37z5h9 53 9 joining join VBG cord-016755-ye37z5h9 53 10 method method NN cord-016755-ye37z5h9 53 11 using use VBG cord-016755-ye37z5h9 53 12 the the DT cord-016755-ye37z5h9 53 13 Poisson Poisson NNP cord-016755-ye37z5h9 53 14 correction correction NN cord-016755-ye37z5h9 53 15 and and CC cord-016755-ye37z5h9 53 16 complete complete JJ cord-016755-ye37z5h9 53 17 deletion deletion NN cord-016755-ye37z5h9 53 18 of of IN cord-016755-ye37z5h9 53 19 gaps gap NNS cord-016755-ye37z5h9 53 20 . . . cord-016755-ye37z5h9 54 1 Bootstrap bootstrap NN cord-016755-ye37z5h9 54 2 values value NNS cord-016755-ye37z5h9 54 3 were be VBD cord-016755-ye37z5h9 54 4 estimated estimate VBN cord-016755-ye37z5h9 54 5 from from IN cord-016755-ye37z5h9 54 6 2000 2000 CD cord-016755-ye37z5h9 54 7 replicates replicate NNS cord-016755-ye37z5h9 54 8 ( ( -LRB- cord-016755-ye37z5h9 54 9 95 95 CD cord-016755-ye37z5h9 54 10 % % NN cord-016755-ye37z5h9 54 11 confidence confidence NN cord-016755-ye37z5h9 54 12 ) ) -RRB- cord-016755-ye37z5h9 54 13 with with IN cord-016755-ye37z5h9 54 14 a a DT cord-016755-ye37z5h9 54 15 random random JJ cord-016755-ye37z5h9 54 16 seed seed NN cord-016755-ye37z5h9 54 17 . . . cord-016755-ye37z5h9 55 1 Phylogenetic phylogenetic JJ cord-016755-ye37z5h9 55 2 trees tree NNS cord-016755-ye37z5h9 55 3 based base VBN cord-016755-ye37z5h9 55 4 on on IN cord-016755-ye37z5h9 55 5 complete complete JJ cord-016755-ye37z5h9 55 6 viral viral JJ cord-016755-ye37z5h9 55 7 genomic genomic JJ cord-016755-ye37z5h9 55 8 sequence sequence NN cord-016755-ye37z5h9 55 9 of of IN cord-016755-ye37z5h9 55 10 L L NNP cord-016755-ye37z5h9 55 11 , , , cord-016755-ye37z5h9 55 12 M M NNP cord-016755-ye37z5h9 55 13 and and CC cord-016755-ye37z5h9 55 14 S S NNP cord-016755-ye37z5h9 55 15 segments segment NNS cord-016755-ye37z5h9 55 16 from from IN cord-016755-ye37z5h9 55 17 strains strain NNS cord-016755-ye37z5h9 55 18 ( ( -LRB- cord-016755-ye37z5h9 55 19 HB29 HB29 NNP cord-016755-ye37z5h9 55 20 , , , cord-016755-ye37z5h9 55 21 HN6 HN6 NNP cord-016755-ye37z5h9 55 22 , , , cord-016755-ye37z5h9 55 23 AN12 AN12 NNP cord-016755-ye37z5h9 55 24 , , , cord-016755-ye37z5h9 55 25 LN2 LN2 NNP cord-016755-ye37z5h9 55 26 , , , cord-016755-ye37z5h9 55 27 JS3 JS3 NNP cord-016755-ye37z5h9 55 28 and and CC cord-016755-ye37z5h9 55 29 SD4 SD4 NNP cord-016755-ye37z5h9 55 30 ) ) -RRB- cord-016755-ye37z5h9 55 31 from from IN cord-016755-ye37z5h9 55 32 6 6 CD cord-016755-ye37z5h9 55 33 provinces province NNS cord-016755-ye37z5h9 55 34 in in IN cord-016755-ye37z5h9 55 35 China China NNP cord-016755-ye37z5h9 55 36 in in IN cord-016755-ye37z5h9 55 37 comparison comparison NN cord-016755-ye37z5h9 55 38 with with IN cord-016755-ye37z5h9 55 39 other other JJ cord-016755-ye37z5h9 55 40 known know VBN cord-016755-ye37z5h9 55 41 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 55 42 showed show VBD cord-016755-ye37z5h9 55 43 that that IN cord-016755-ye37z5h9 55 44 SFTS SFTS NNP cord-016755-ye37z5h9 55 45 virus virus NN cord-016755-ye37z5h9 55 46 was be VBD cord-016755-ye37z5h9 55 47 related relate VBN cord-016755-ye37z5h9 55 48 to to IN cord-016755-ye37z5h9 55 49 prototypic prototypic NNP cord-016755-ye37z5h9 55 50 viruses virus NNS cord-016755-ye37z5h9 55 51 of of IN cord-016755-ye37z5h9 55 52 Phlebovirus Phlebovirus NNP cord-016755-ye37z5h9 55 53 . . . cord-016755-ye37z5h9 56 1 Phylogenetic phylogenetic JJ cord-016755-ye37z5h9 56 2 analysis analysis NN cord-016755-ye37z5h9 56 3 using use VBG cord-016755-ye37z5h9 56 4 the the DT cord-016755-ye37z5h9 56 5 deduced deduce VBN cord-016755-ye37z5h9 56 6 amino amino NN cord-016755-ye37z5h9 56 7 acid acid NN cord-016755-ye37z5h9 56 8 sequences sequence NNS cord-016755-ye37z5h9 56 9 of of IN cord-016755-ye37z5h9 56 10 RdRp RdRp NNP cord-016755-ye37z5h9 56 11 , , , cord-016755-ye37z5h9 56 12 glycoproteins glycoprotein NNS cord-016755-ye37z5h9 56 13 ( ( -LRB- cord-016755-ye37z5h9 56 14 Gn Gn NNP cord-016755-ye37z5h9 56 15 and and CC cord-016755-ye37z5h9 56 16 Gc Gc NNP cord-016755-ye37z5h9 56 17 ) ) -RRB- cord-016755-ye37z5h9 56 18 , , , cord-016755-ye37z5h9 56 19 N N NNP cord-016755-ye37z5h9 56 20 and and CC cord-016755-ye37z5h9 56 21 NSs NSs NNP cord-016755-ye37z5h9 56 22 proteins protein NNS cord-016755-ye37z5h9 57 1 , , , cord-016755-ye37z5h9 57 2 the the DT cord-016755-ye37z5h9 57 3 generated generate VBN cord-016755-ye37z5h9 57 4 phylogenetic phylogenetic JJ cord-016755-ye37z5h9 57 5 tree tree NN cord-016755-ye37z5h9 57 6 showed show VBD cord-016755-ye37z5h9 57 7 all all DT cord-016755-ye37z5h9 57 8 SFTS SFTS NNP cord-016755-ye37z5h9 57 9 virus virus NN cord-016755-ye37z5h9 57 10 isolates isolate VBZ cord-016755-ye37z5h9 57 11 clustered cluster VBN cord-016755-ye37z5h9 57 12 together together RB cord-016755-ye37z5h9 57 13 , , , cord-016755-ye37z5h9 57 14 but but CC cord-016755-ye37z5h9 57 15 are be VBP cord-016755-ye37z5h9 57 16 almost almost RB cord-016755-ye37z5h9 57 17 equally equally RB cord-016755-ye37z5h9 57 18 distant distant JJ cord-016755-ye37z5h9 57 19 from from IN cord-016755-ye37z5h9 57 20 other other JJ cord-016755-ye37z5h9 57 21 two two CD cord-016755-ye37z5h9 57 22 groups group NNS cord-016755-ye37z5h9 58 1 ( ( -LRB- cord-016755-ye37z5h9 58 2 Fisher Fisher NNP cord-016755-ye37z5h9 58 3 et et NNP cord-016755-ye37z5h9 58 4 al al NNP cord-016755-ye37z5h9 58 5 . . NNP cord-016755-ye37z5h9 58 6 2003 2003 CD cord-016755-ye37z5h9 58 7 ) ) -RRB- cord-016755-ye37z5h9 58 8 , , , cord-016755-ye37z5h9 58 9 including include VBG cord-016755-ye37z5h9 58 10 the the DT cord-016755-ye37z5h9 58 11 sandfly sandfly NNP cord-016755-ye37z5h9 58 12 fever fever NNP cord-016755-ye37z5h9 58 13 group group NNP cord-016755-ye37z5h9 58 14 ( ( -LRB- cord-016755-ye37z5h9 58 15 Rift Rift NNP cord-016755-ye37z5h9 58 16 Valley Valley NNP cord-016755-ye37z5h9 58 17 fever fever NN cord-016755-ye37z5h9 58 18 , , , cord-016755-ye37z5h9 58 19 Punta Punta NNP cord-016755-ye37z5h9 58 20 Toro Toro NNP cord-016755-ye37z5h9 58 21 , , , cord-016755-ye37z5h9 58 22 Toscana Toscana NNP cord-016755-ye37z5h9 58 23 , , , cord-016755-ye37z5h9 58 24 Massila Massila NNP cord-016755-ye37z5h9 58 25 , , , cord-016755-ye37z5h9 58 26 sandfly sandfly NN cord-016755-ye37z5h9 58 27 fever fever NN cord-016755-ye37z5h9 58 28 Sicilian sicilian JJ cord-016755-ye37z5h9 58 29 viruses virus NNS cord-016755-ye37z5h9 58 30 ) ) -RRB- cord-016755-ye37z5h9 58 31 and and CC cord-016755-ye37z5h9 58 32 Uukuniemi Uukuniemi NNP cord-016755-ye37z5h9 58 33 group group NN cord-016755-ye37z5h9 58 34 . . . cord-016755-ye37z5h9 59 1 The the DT cord-016755-ye37z5h9 59 2 comparison comparison NN cord-016755-ye37z5h9 59 3 of of IN cord-016755-ye37z5h9 59 4 amino amino NN cord-016755-ye37z5h9 59 5 acid acid NN cord-016755-ye37z5h9 59 6 sequence sequence NN cord-016755-ye37z5h9 59 7 similarity similarity NN cord-016755-ye37z5h9 59 8 provided provide VBD cord-016755-ye37z5h9 59 9 further further JJ cord-016755-ye37z5h9 59 10 support support NN cord-016755-ye37z5h9 59 11 to to IN cord-016755-ye37z5h9 59 12 the the DT cord-016755-ye37z5h9 59 13 separation separation NN cord-016755-ye37z5h9 59 14 of of IN cord-016755-ye37z5h9 59 15 SFTS SFTS NNP cord-016755-ye37z5h9 59 16 virus virus NN cord-016755-ye37z5h9 59 17 from from IN cord-016755-ye37z5h9 59 18 other other JJ cord-016755-ye37z5h9 59 19 phleboviruses phleboviruse NNS cord-016755-ye37z5h9 59 20 . . . cord-016755-ye37z5h9 60 1 SFTS SFTS NNP cord-016755-ye37z5h9 60 2 virus virus NN cord-016755-ye37z5h9 60 3 was be VBD cord-016755-ye37z5h9 60 4 assigned assign VBN cord-016755-ye37z5h9 60 5 to to TO cord-016755-ye37z5h9 60 6 be be VB cord-016755-ye37z5h9 60 7 a a DT cord-016755-ye37z5h9 60 8 novel novel JJ cord-016755-ye37z5h9 60 9 species specie NNS cord-016755-ye37z5h9 60 10 at at IN cord-016755-ye37z5h9 60 11 the the DT cord-016755-ye37z5h9 60 12 genus genus NN cord-016755-ye37z5h9 60 13 of of IN cord-016755-ye37z5h9 60 14 Phlebovirus Phlebovirus NNP cord-016755-ye37z5h9 60 15 in in IN cord-016755-ye37z5h9 60 16 Bunyaviriade Bunyaviriade NNP cord-016755-ye37z5h9 60 17 in in IN cord-016755-ye37z5h9 60 18 2014 2014 CD cord-016755-ye37z5h9 60 19 , , , cord-016755-ye37z5h9 60 20 and and CC cord-016755-ye37z5h9 60 21 was be VBD cord-016755-ye37z5h9 60 22 resigned resign VBN cord-016755-ye37z5h9 60 23 to to TO cord-016755-ye37z5h9 60 24 be be VB cord-016755-ye37z5h9 60 25 a a DT cord-016755-ye37z5h9 60 26 novel novel JJ cord-016755-ye37z5h9 60 27 species specie NNS cord-016755-ye37z5h9 60 28 at at IN cord-016755-ye37z5h9 60 29 the the DT cord-016755-ye37z5h9 60 30 genus genus NN cord-016755-ye37z5h9 60 31 of of IN cord-016755-ye37z5h9 60 32 Phlebovirus Phlebovirus NNP cord-016755-ye37z5h9 60 33 in in IN cord-016755-ye37z5h9 60 34 the the DT cord-016755-ye37z5h9 60 35 family family NN cord-016755-ye37z5h9 60 36 of of IN cord-016755-ye37z5h9 60 37 Phenuiviridae Phenuiviridae NNP cord-016755-ye37z5h9 60 38 by by IN cord-016755-ye37z5h9 60 39 International International NNP cord-016755-ye37z5h9 60 40 Committee Committee NNP cord-016755-ye37z5h9 60 41 on on IN cord-016755-ye37z5h9 60 42 Taxonomy Taxonomy NNP cord-016755-ye37z5h9 60 43 of of IN cord-016755-ye37z5h9 60 44 Viruses Viruses NNPS cord-016755-ye37z5h9 60 45 ( ( -LRB- cord-016755-ye37z5h9 60 46 ICTV ICTV NNP cord-016755-ye37z5h9 60 47 ) ) -RRB- cord-016755-ye37z5h9 60 48 in in IN cord-016755-ye37z5h9 60 49 2016 2016 CD cord-016755-ye37z5h9 60 50 . . . cord-016755-ye37z5h9 61 1 In in IN cord-016755-ye37z5h9 61 2 June June NNP cord-016755-ye37z5h9 61 3 , , , cord-016755-ye37z5h9 61 4 2009 2009 CD cord-016755-ye37z5h9 61 5 , , , cord-016755-ye37z5h9 61 6 a a DT cord-016755-ye37z5h9 61 7 blood blood NN cord-016755-ye37z5h9 61 8 sample sample NN cord-016755-ye37z5h9 61 9 in in IN cord-016755-ye37z5h9 61 10 heparin heparin NN cord-016755-ye37z5h9 61 11 anticoagulant anticoagulant NN cord-016755-ye37z5h9 61 12 was be VBD cord-016755-ye37z5h9 61 13 collected collect VBN cord-016755-ye37z5h9 61 14 on on IN cord-016755-ye37z5h9 61 15 day day NN cord-016755-ye37z5h9 61 16 7 7 CD cord-016755-ye37z5h9 61 17 after after IN cord-016755-ye37z5h9 61 18 onset onset NN cord-016755-ye37z5h9 61 19 of of IN cord-016755-ye37z5h9 61 20 illness illness NN cord-016755-ye37z5h9 61 21 from from IN cord-016755-ye37z5h9 61 22 a a DT cord-016755-ye37z5h9 61 23 patient patient NN cord-016755-ye37z5h9 61 24 from from IN cord-016755-ye37z5h9 61 25 Xinyang Xinyang NNP cord-016755-ye37z5h9 61 26 City City NNP cord-016755-ye37z5h9 61 27 , , , cord-016755-ye37z5h9 61 28 Henan Henan NNP cord-016755-ye37z5h9 61 29 Province Province NNP cord-016755-ye37z5h9 61 30 . . . cord-016755-ye37z5h9 62 1 Due due IN cord-016755-ye37z5h9 62 2 to to IN cord-016755-ye37z5h9 62 3 the the DT cord-016755-ye37z5h9 62 4 lack lack NN cord-016755-ye37z5h9 62 5 of of IN cord-016755-ye37z5h9 62 6 knowledge knowledge NN cord-016755-ye37z5h9 62 7 on on IN cord-016755-ye37z5h9 62 8 the the DT cord-016755-ye37z5h9 62 9 causes cause NNS cord-016755-ye37z5h9 62 10 of of IN cord-016755-ye37z5h9 62 11 the the DT cord-016755-ye37z5h9 62 12 illness illness NN cord-016755-ye37z5h9 62 13 , , , cord-016755-ye37z5h9 62 14 the the DT cord-016755-ye37z5h9 62 15 pathogen pathogen NN cord-016755-ye37z5h9 62 16 was be VBD cord-016755-ye37z5h9 62 17 isolated isolate VBN cord-016755-ye37z5h9 62 18 by by IN cord-016755-ye37z5h9 62 19 inoculating inoculate VBG cord-016755-ye37z5h9 62 20 multiple multiple JJ cord-016755-ye37z5h9 62 21 cell cell NN cord-016755-ye37z5h9 62 22 lines line NNS cord-016755-ye37z5h9 62 23 susceptible susceptible JJ cord-016755-ye37z5h9 62 24 to to IN cord-016755-ye37z5h9 62 25 both both CC cord-016755-ye37z5h9 62 26 viral viral JJ cord-016755-ye37z5h9 62 27 and and CC cord-016755-ye37z5h9 62 28 rickettsial rickettsial JJ cord-016755-ye37z5h9 62 29 agents agent NNS cord-016755-ye37z5h9 62 30 including include VBG cord-016755-ye37z5h9 62 31 DH82 DH82 NNP cord-016755-ye37z5h9 62 32 , , , cord-016755-ye37z5h9 62 33 L929 l929 CD cord-016755-ye37z5h9 62 34 , , , cord-016755-ye37z5h9 62 35 Vero Vero NNP cord-016755-ye37z5h9 62 36 , , , cord-016755-ye37z5h9 62 37 Vero Vero NNP cord-016755-ye37z5h9 62 38 E6 E6 NNP cord-016755-ye37z5h9 62 39 , , , cord-016755-ye37z5h9 62 40 HL60 HL60 NNP cord-016755-ye37z5h9 62 41 cells cell NNS cord-016755-ye37z5h9 62 42 , , , cord-016755-ye37z5h9 62 43 and and CC cord-016755-ye37z5h9 62 44 the the DT cord-016755-ye37z5h9 62 45 tick tick NN cord-016755-ye37z5h9 62 46 cell cell NN cord-016755-ye37z5h9 62 47 line line NN cord-016755-ye37z5h9 62 48 ISE6 ISE6 NNP cord-016755-ye37z5h9 62 49 . . . cord-016755-ye37z5h9 63 1 Patients patient NNS cord-016755-ye37z5h9 63 2 ' ' POS cord-016755-ye37z5h9 63 3 white white JJ cord-016755-ye37z5h9 63 4 blood blood NN cord-016755-ye37z5h9 63 5 cells cell NNS cord-016755-ye37z5h9 63 6 were be VBD cord-016755-ye37z5h9 63 7 inoculated inoculate VBN cord-016755-ye37z5h9 63 8 to to IN cord-016755-ye37z5h9 63 9 the the DT cord-016755-ye37z5h9 63 10 cell cell NN cord-016755-ye37z5h9 63 11 monolayers monolayer NNS cord-016755-ye37z5h9 63 12 . . . cord-016755-ye37z5h9 64 1 The the DT cord-016755-ye37z5h9 64 2 cells cell NNS cord-016755-ye37z5h9 64 3 were be VBD cord-016755-ye37z5h9 64 4 cultured culture VBN cord-016755-ye37z5h9 64 5 at at IN cord-016755-ye37z5h9 64 6 37 37 CD cord-016755-ye37z5h9 64 7 ° ° NN cord-016755-ye37z5h9 64 8 C c NN cord-016755-ye37z5h9 64 9 in in IN cord-016755-ye37z5h9 64 10 a a DT cord-016755-ye37z5h9 64 11 5 5 CD cord-016755-ye37z5h9 64 12 % % NN cord-016755-ye37z5h9 64 13 CO co NN cord-016755-ye37z5h9 64 14 2 2 CD cord-016755-ye37z5h9 64 15 atmosphere atmosphere NN cord-016755-ye37z5h9 64 16 with with IN cord-016755-ye37z5h9 64 17 medium medium JJ cord-016755-ye37z5h9 64 18 changes change NNS cord-016755-ye37z5h9 64 19 twice twice RB cord-016755-ye37z5h9 64 20 a a DT cord-016755-ye37z5h9 64 21 week week NN cord-016755-ye37z5h9 64 22 . . . cord-016755-ye37z5h9 65 1 A a DT cord-016755-ye37z5h9 65 2 month month NN cord-016755-ye37z5h9 65 3 after after IN cord-016755-ye37z5h9 65 4 inoculation inoculation NN cord-016755-ye37z5h9 65 5 of of IN cord-016755-ye37z5h9 65 6 the the DT cord-016755-ye37z5h9 65 7 cells cell NNS cord-016755-ye37z5h9 65 8 with with IN cord-016755-ye37z5h9 65 9 white white JJ cord-016755-ye37z5h9 65 10 blood blood NN cord-016755-ye37z5h9 65 11 cell cell NN cord-016755-ye37z5h9 65 12 ( ( -LRB- cord-016755-ye37z5h9 65 13 WBC WBC NNP cord-016755-ye37z5h9 65 14 ) ) -RRB- cord-016755-ye37z5h9 65 15 of of IN cord-016755-ye37z5h9 65 16 the the DT cord-016755-ye37z5h9 65 17 patient patient NN cord-016755-ye37z5h9 65 18 , , , cord-016755-ye37z5h9 65 19 cytopathic cytopathic JJ cord-016755-ye37z5h9 65 20 effect effect NN cord-016755-ye37z5h9 65 21 ( ( -LRB- cord-016755-ye37z5h9 65 22 CPE CPE NNP cord-016755-ye37z5h9 65 23 ) ) -RRB- cord-016755-ye37z5h9 65 24 was be VBD cord-016755-ye37z5h9 65 25 observed observe VBN cord-016755-ye37z5h9 65 26 in in IN cord-016755-ye37z5h9 65 27 the the DT cord-016755-ye37z5h9 65 28 DH82 DH82 NNP cord-016755-ye37z5h9 65 29 cells cell NNS cord-016755-ye37z5h9 65 30 . . . cord-016755-ye37z5h9 66 1 The the DT cord-016755-ye37z5h9 66 2 morphology morphology NN cord-016755-ye37z5h9 66 3 of of IN cord-016755-ye37z5h9 66 4 infected infected JJ cord-016755-ye37z5h9 66 5 DH82 DH82 NNP cord-016755-ye37z5h9 66 6 cells cell NNS cord-016755-ye37z5h9 66 7 changed change VBN cord-016755-ye37z5h9 66 8 from from IN cord-016755-ye37z5h9 66 9 round round JJ cord-016755-ye37z5h9 66 10 monocytes monocyte NNS cord-016755-ye37z5h9 66 11 to to IN cord-016755-ye37z5h9 66 12 a a DT cord-016755-ye37z5h9 66 13 fibroblast fibroblast NN cord-016755-ye37z5h9 66 14 - - HYPH cord-016755-ye37z5h9 66 15 like like JJ cord-016755-ye37z5h9 66 16 shape shape NN cord-016755-ye37z5h9 66 17 , , , cord-016755-ye37z5h9 66 18 which which WDT cord-016755-ye37z5h9 66 19 had have VBD cord-016755-ye37z5h9 66 20 granular granular JJ cord-016755-ye37z5h9 66 21 particles particle NNS cord-016755-ye37z5h9 66 22 in in IN cord-016755-ye37z5h9 66 23 the the DT cord-016755-ye37z5h9 66 24 cytoplasm cytoplasm NN cord-016755-ye37z5h9 66 25 . . . cord-016755-ye37z5h9 67 1 In in IN cord-016755-ye37z5h9 67 2 continuous continuous JJ cord-016755-ye37z5h9 67 3 culture culture NN cord-016755-ye37z5h9 67 4 , , , cord-016755-ye37z5h9 67 5 the the DT cord-016755-ye37z5h9 67 6 CPE CPE NNP cord-016755-ye37z5h9 67 7 usually usually RB cord-016755-ye37z5h9 67 8 appeared appear VBD cord-016755-ye37z5h9 67 9 on on IN cord-016755-ye37z5h9 67 10 day day NN cord-016755-ye37z5h9 67 11 4 4 CD cord-016755-ye37z5h9 67 12 after after IN cord-016755-ye37z5h9 67 13 inoculation inoculation NN cord-016755-ye37z5h9 67 14 of of IN cord-016755-ye37z5h9 67 15 a a DT cord-016755-ye37z5h9 67 16 fresh fresh JJ cord-016755-ye37z5h9 67 17 monolayer monolayer NN cord-016755-ye37z5h9 67 18 . . . cord-016755-ye37z5h9 68 1 But but CC cord-016755-ye37z5h9 68 2 the the DT cord-016755-ye37z5h9 68 3 pathogen pathogen NN cord-016755-ye37z5h9 68 4 was be VBD cord-016755-ye37z5h9 68 5 not not RB cord-016755-ye37z5h9 68 6 characterized characterize VBN cord-016755-ye37z5h9 68 7 clearly clearly RB cord-016755-ye37z5h9 68 8 . . . cord-016755-ye37z5h9 69 1 Subsequently subsequently RB cord-016755-ye37z5h9 69 2 , , , cord-016755-ye37z5h9 69 3 the the DT cord-016755-ye37z5h9 69 4 virus virus NN cord-016755-ye37z5h9 69 5 was be VBD cord-016755-ye37z5h9 69 6 isolated isolate VBN cord-016755-ye37z5h9 69 7 by by IN cord-016755-ye37z5h9 69 8 inoculation inoculation NN cord-016755-ye37z5h9 69 9 of of IN cord-016755-ye37z5h9 69 10 Vero Vero NNP cord-016755-ye37z5h9 69 11 cells cell NNS cord-016755-ye37z5h9 69 12 with with IN cord-016755-ye37z5h9 69 13 serum serum NN cord-016755-ye37z5h9 69 14 or or CC cord-016755-ye37z5h9 69 15 homogenized homogenized JJ cord-016755-ye37z5h9 69 16 WBCs wbc NNS cord-016755-ye37z5h9 69 17 in in IN cord-016755-ye37z5h9 69 18 2010 2010 CD cord-016755-ye37z5h9 69 19 . . . cord-016755-ye37z5h9 70 1 After after IN cord-016755-ye37z5h9 70 2 incubation incubation NN cord-016755-ye37z5h9 70 3 for for IN cord-016755-ye37z5h9 70 4 10 10 CD cord-016755-ye37z5h9 70 5 - - SYM cord-016755-ye37z5h9 70 6 14 14 CD cord-016755-ye37z5h9 70 7 days day NNS cord-016755-ye37z5h9 70 8 at at IN cord-016755-ye37z5h9 70 9 37 37 CD cord-016755-ye37z5h9 70 10 ° ° NN cord-016755-ye37z5h9 70 11 C c NN cord-016755-ye37z5h9 70 12 , , , cord-016755-ye37z5h9 70 13 culture culture NN cord-016755-ye37z5h9 70 14 fluid fluid NN cord-016755-ye37z5h9 70 15 was be VBD cord-016755-ye37z5h9 70 16 passaged passage VBN cord-016755-ye37z5h9 70 17 to to IN cord-016755-ye37z5h9 70 18 new new JJ cord-016755-ye37z5h9 70 19 monolayers monolayer NNS cord-016755-ye37z5h9 70 20 . . . cord-016755-ye37z5h9 71 1 Cells cell NNS cord-016755-ye37z5h9 71 2 were be VBD cord-016755-ye37z5h9 71 3 monitored monitor VBN cord-016755-ye37z5h9 71 4 daily daily RB cord-016755-ye37z5h9 71 5 for for IN cord-016755-ye37z5h9 71 6 presence presence NN cord-016755-ye37z5h9 71 7 of of IN cord-016755-ye37z5h9 71 8 viral viral JJ cord-016755-ye37z5h9 71 9 RNA RNA NNP cord-016755-ye37z5h9 71 10 in in IN cord-016755-ye37z5h9 71 11 supernatant supernatant VBN cord-016755-ye37z5h9 71 12 by by IN cord-016755-ye37z5h9 71 13 real real JJ cord-016755-ye37z5h9 71 14 - - HYPH cord-016755-ye37z5h9 71 15 time time NN cord-016755-ye37z5h9 71 16 PCR PCR NNP cord-016755-ye37z5h9 71 17 . . . cord-016755-ye37z5h9 72 1 The the DT cord-016755-ye37z5h9 72 2 novel novel NN cord-016755-ye37z5h9 72 3 phlebovirus phlebovirus NN cord-016755-ye37z5h9 72 4 was be VBD cord-016755-ye37z5h9 72 5 isolated isolate VBN cord-016755-ye37z5h9 72 6 in in IN cord-016755-ye37z5h9 72 7 cultured cultured JJ cord-016755-ye37z5h9 72 8 Vero Vero NNP cord-016755-ye37z5h9 72 9 cells cell NNS cord-016755-ye37z5h9 72 10 inoculated inoculate VBN cord-016755-ye37z5h9 72 11 with with IN cord-016755-ye37z5h9 72 12 acute acute JJ cord-016755-ye37z5h9 72 13 - - HYPH cord-016755-ye37z5h9 72 14 phase phase NN cord-016755-ye37z5h9 72 15 serum serum NN cord-016755-ye37z5h9 72 16 of of IN cord-016755-ye37z5h9 72 17 patient patient JJ cord-016755-ye37z5h9 72 18 HB29 HB29 NNP cord-016755-ye37z5h9 72 19 from from IN cord-016755-ye37z5h9 72 20 Shuizhou Shuizhou NNP cord-016755-ye37z5h9 72 21 area area NN cord-016755-ye37z5h9 72 22 , , , cord-016755-ye37z5h9 72 23 Hubei Hubei NNP cord-016755-ye37z5h9 72 24 province province NN cord-016755-ye37z5h9 72 25 . . . cord-016755-ye37z5h9 73 1 Virus virus NN cord-016755-ye37z5h9 73 2 isolation isolation NN cord-016755-ye37z5h9 73 3 was be VBD cord-016755-ye37z5h9 73 4 also also RB cord-016755-ye37z5h9 73 5 successful successful JJ cord-016755-ye37z5h9 73 6 with with IN cord-016755-ye37z5h9 73 7 sera sera NNP cord-016755-ye37z5h9 73 8 from from IN cord-016755-ye37z5h9 73 9 other other JJ cord-016755-ye37z5h9 73 10 10 10 CD cord-016755-ye37z5h9 73 11 patients patient NNS cord-016755-ye37z5h9 73 12 with with IN cord-016755-ye37z5h9 73 13 SFTS SFTS NNP cord-016755-ye37z5h9 73 14 from from IN cord-016755-ye37z5h9 73 15 6 6 CD cord-016755-ye37z5h9 73 16 provinces province NNS cord-016755-ye37z5h9 73 17 . . . cord-016755-ye37z5h9 74 1 Viral Viral NNP cord-016755-ye37z5h9 74 2 RNA RNA NNP cord-016755-ye37z5h9 74 3 was be VBD cord-016755-ye37z5h9 74 4 detected detect VBN cord-016755-ye37z5h9 74 5 by by IN cord-016755-ye37z5h9 74 6 real real JJ cord-016755-ye37z5h9 74 7 - - HYPH cord-016755-ye37z5h9 74 8 time time NN cord-016755-ye37z5h9 74 9 RT RT NNP cord-016755-ye37z5h9 74 10 - - HYPH cord-016755-ye37z5h9 74 11 PCR PCR NNP cord-016755-ye37z5h9 74 12 on on IN cord-016755-ye37z5h9 74 13 day day NN cord-016755-ye37z5h9 74 14 7 7 CD cord-016755-ye37z5h9 74 15 after after IN cord-016755-ye37z5h9 74 16 inoculation inoculation NN cord-016755-ye37z5h9 74 17 . . . cord-016755-ye37z5h9 75 1 Immunoreactivity Immunoreactivity NNP cord-016755-ye37z5h9 75 2 of of IN cord-016755-ye37z5h9 75 3 infected infected JJ cord-016755-ye37z5h9 75 4 cells cell NNS cord-016755-ye37z5h9 75 5 with with IN cord-016755-ye37z5h9 75 6 convalescent convalescent JJ cord-016755-ye37z5h9 75 7 - - HYPH cord-016755-ye37z5h9 75 8 phase phase NN cord-016755-ye37z5h9 75 9 serum serum NN cord-016755-ye37z5h9 75 10 collected collect VBN cord-016755-ye37z5h9 75 11 from from IN cord-016755-ye37z5h9 75 12 RT RT NNP cord-016755-ye37z5h9 75 13 - - HYPH cord-016755-ye37z5h9 75 14 PCR PCR NNP cord-016755-ye37z5h9 75 15 confirmed confirm VBD cord-016755-ye37z5h9 75 16 patients patient NNS cord-016755-ye37z5h9 75 17 was be VBD cord-016755-ye37z5h9 75 18 observed observe VBN cord-016755-ye37z5h9 75 19 as as IN cord-016755-ye37z5h9 75 20 punctate punctate NN cord-016755-ye37z5h9 75 21 staining staining NN cord-016755-ye37z5h9 75 22 pattern pattern NN cord-016755-ye37z5h9 75 23 distributed distribute VBN cord-016755-ye37z5h9 75 24 evenly evenly RB cord-016755-ye37z5h9 75 25 throughout throughout IN cord-016755-ye37z5h9 75 26 the the DT cord-016755-ye37z5h9 75 27 infected infected JJ cord-016755-ye37z5h9 75 28 cells cell NNS cord-016755-ye37z5h9 75 29 at at IN cord-016755-ye37z5h9 75 30 day day NN cord-016755-ye37z5h9 75 31 10 10 CD cord-016755-ye37z5h9 75 32 after after IN cord-016755-ye37z5h9 75 33 inoculation inoculation NN cord-016755-ye37z5h9 75 34 . . . cord-016755-ye37z5h9 76 1 A a DT cord-016755-ye37z5h9 76 2 plaque plaque NN cord-016755-ye37z5h9 76 3 assay assay NN cord-016755-ye37z5h9 76 4 was be VBD cord-016755-ye37z5h9 76 5 performed perform VBN cord-016755-ye37z5h9 76 6 on on IN cord-016755-ye37z5h9 76 7 Vero Vero NNP cord-016755-ye37z5h9 76 8 cells cell NNS cord-016755-ye37z5h9 76 9 to to TO cord-016755-ye37z5h9 76 10 quantify quantify VB cord-016755-ye37z5h9 76 11 the the DT cord-016755-ye37z5h9 76 12 infectious infectious JJ cord-016755-ye37z5h9 76 13 particles particle NNS cord-016755-ye37z5h9 76 14 in in IN cord-016755-ye37z5h9 76 15 the the DT cord-016755-ye37z5h9 76 16 supernatant supernatant NN cord-016755-ye37z5h9 76 17 . . . cord-016755-ye37z5h9 77 1 The the DT cord-016755-ye37z5h9 77 2 plaques plaque NNS cord-016755-ye37z5h9 77 3 were be VBD cord-016755-ye37z5h9 77 4 visualized visualize VBN cord-016755-ye37z5h9 77 5 by by IN cord-016755-ye37z5h9 77 6 staining stain VBG cord-016755-ye37z5h9 77 7 with with IN cord-016755-ye37z5h9 77 8 3 3 CD cord-016755-ye37z5h9 77 9 % % NN cord-016755-ye37z5h9 77 10 neutral neutral JJ cord-016755-ye37z5h9 77 11 red red JJ cord-016755-ye37z5h9 77 12 solution solution NN cord-016755-ye37z5h9 77 13 in in IN cord-016755-ye37z5h9 77 14 molten molten JJ cord-016755-ye37z5h9 77 15 agarose agarose NN cord-016755-ye37z5h9 77 16 . . . cord-016755-ye37z5h9 78 1 The the DT cord-016755-ye37z5h9 78 2 virus virus NN cord-016755-ye37z5h9 78 3 titer titer NN cord-016755-ye37z5h9 78 4 was be VBD cord-016755-ye37z5h9 78 5 about about RB cord-016755-ye37z5h9 78 6 6 6 CD cord-016755-ye37z5h9 78 7 × × NN cord-016755-ye37z5h9 78 8 10 10 CD cord-016755-ye37z5h9 78 9 7 7 CD cord-016755-ye37z5h9 78 10 pfu pfu NN cord-016755-ye37z5h9 78 11 / / SYM cord-016755-ye37z5h9 78 12 mL ml NN cord-016755-ye37z5h9 78 13 in in IN cord-016755-ye37z5h9 78 14 the the DT cord-016755-ye37z5h9 78 15 culture culture NN cord-016755-ye37z5h9 78 16 supernatant supernatant JJ cord-016755-ye37z5h9 78 17 at at IN cord-016755-ye37z5h9 78 18 the the DT cord-016755-ye37z5h9 78 19 2 2 CD cord-016755-ye37z5h9 78 20 - - SYM cord-016755-ye37z5h9 78 21 3 3 CD cord-016755-ye37z5h9 78 22 passage passage NN cord-016755-ye37z5h9 78 23 of of IN cord-016755-ye37z5h9 78 24 the the DT cord-016755-ye37z5h9 78 25 virus virus NN cord-016755-ye37z5h9 78 26 . . . cord-016755-ye37z5h9 79 1 Negative negative JJ cord-016755-ye37z5h9 79 2 - - HYPH cord-016755-ye37z5h9 79 3 stain stain NN cord-016755-ye37z5h9 79 4 electron electron NN cord-016755-ye37z5h9 79 5 microscopy microscopy NN cord-016755-ye37z5h9 79 6 revealed reveal VBD cord-016755-ye37z5h9 79 7 SFTS SFTS NNP cord-016755-ye37z5h9 79 8 virus virus NN cord-016755-ye37z5h9 79 9 particles particle NNS cord-016755-ye37z5h9 79 10 of of IN cord-016755-ye37z5h9 79 11 80 80 CD cord-016755-ye37z5h9 79 12 - - SYM cord-016755-ye37z5h9 79 13 100 100 CD cord-016755-ye37z5h9 79 14 nm nm NN cord-016755-ye37z5h9 79 15 in in IN cord-016755-ye37z5h9 79 16 diameter diameter NN cord-016755-ye37z5h9 79 17 , , , cord-016755-ye37z5h9 79 18 with with IN cord-016755-ye37z5h9 79 19 complex complex JJ cord-016755-ye37z5h9 79 20 surface surface NN cord-016755-ye37z5h9 79 21 projections projection NNS cord-016755-ye37z5h9 79 22 surrounding surround VBG cord-016755-ye37z5h9 79 23 the the DT cord-016755-ye37z5h9 79 24 periphery periphery NN cord-016755-ye37z5h9 79 25 . . . cord-016755-ye37z5h9 80 1 Transmission transmission NN cord-016755-ye37z5h9 80 2 electron electron NN cord-016755-ye37z5h9 80 3 microscopy microscopy NN cord-016755-ye37z5h9 80 4 ( ( -LRB- cord-016755-ye37z5h9 80 5 TEM TEM NNP cord-016755-ye37z5h9 80 6 ) ) -RRB- cord-016755-ye37z5h9 80 7 revealed reveal VBD cord-016755-ye37z5h9 80 8 viral viral JJ cord-016755-ye37z5h9 80 9 particles particle NNS cord-016755-ye37z5h9 80 10 in in IN cord-016755-ye37z5h9 80 11 the the DT cord-016755-ye37z5h9 80 12 DH82 DH82 NNP cord-016755-ye37z5h9 80 13 cell cell NN cord-016755-ye37z5h9 80 14 cytoplasm cytoplasm NN cord-016755-ye37z5h9 80 15 . . . cord-016755-ye37z5h9 81 1 The the DT cord-016755-ye37z5h9 81 2 viruses virus NNS cord-016755-ye37z5h9 81 3 were be VBD cord-016755-ye37z5h9 81 4 observed observe VBN cord-016755-ye37z5h9 81 5 inside inside JJ cord-016755-ye37z5h9 81 6 vacuoles vacuole NNS cord-016755-ye37z5h9 81 7 , , , cord-016755-ye37z5h9 81 8 presumably presumably RB cord-016755-ye37z5h9 81 9 in in IN cord-016755-ye37z5h9 81 10 the the DT cord-016755-ye37z5h9 81 11 Golgi Golgi NNP cord-016755-ye37z5h9 81 12 apparatus apparatus NN cord-016755-ye37z5h9 81 13 . . . cord-016755-ye37z5h9 82 1 Indirect indirect JJ cord-016755-ye37z5h9 82 2 immunofluorescence immunofluorescence NN cord-016755-ye37z5h9 82 3 assay assay NN cord-016755-ye37z5h9 82 4 ( ( -LRB- cord-016755-ye37z5h9 82 5 IFA IFA NNP cord-016755-ye37z5h9 82 6 ) ) -RRB- cord-016755-ye37z5h9 82 7 , , , cord-016755-ye37z5h9 82 8 enzyme enzyme NN cord-016755-ye37z5h9 82 9 - - HYPH cord-016755-ye37z5h9 82 10 linked link VBN cord-016755-ye37z5h9 82 11 immunosorbent immunosorbent JJ cord-016755-ye37z5h9 82 12 assay assay NN cord-016755-ye37z5h9 82 13 ( ( -LRB- cord-016755-ye37z5h9 82 14 ELISA ELISA NNP cord-016755-ye37z5h9 82 15 ) ) -RRB- cord-016755-ye37z5h9 82 16 and and CC cord-016755-ye37z5h9 82 17 micro micro JJ cord-016755-ye37z5h9 82 18 - - NN cord-016755-ye37z5h9 82 19 neutralization neutralization NN cord-016755-ye37z5h9 82 20 test test NN cord-016755-ye37z5h9 82 21 were be VBD cord-016755-ye37z5h9 82 22 used use VBN cord-016755-ye37z5h9 82 23 to to TO cord-016755-ye37z5h9 82 24 detect detect VB cord-016755-ye37z5h9 82 25 the the DT cord-016755-ye37z5h9 82 26 specific specific JJ cord-016755-ye37z5h9 82 27 antibodies antibody NNS cord-016755-ye37z5h9 82 28 in in IN cord-016755-ye37z5h9 82 29 the the DT cord-016755-ye37z5h9 82 30 blood blood NN cord-016755-ye37z5h9 82 31 of of IN cord-016755-ye37z5h9 82 32 patients patient NNS cord-016755-ye37z5h9 82 33 with with IN cord-016755-ye37z5h9 82 34 SFTS SFTS NNP cord-016755-ye37z5h9 82 35 . . . cord-016755-ye37z5h9 83 1 For for IN cord-016755-ye37z5h9 83 2 IFA IFA NNP cord-016755-ye37z5h9 83 3 , , , cord-016755-ye37z5h9 83 4 fixed fix VBN cord-016755-ye37z5h9 83 5 Vero Vero NNP cord-016755-ye37z5h9 83 6 cells cell NNS cord-016755-ye37z5h9 83 7 were be VBD cord-016755-ye37z5h9 83 8 incubated incubate VBN cord-016755-ye37z5h9 83 9 with with IN cord-016755-ye37z5h9 83 10 diluted diluted JJ cord-016755-ye37z5h9 83 11 human human JJ cord-016755-ye37z5h9 83 12 serum serum NN cord-016755-ye37z5h9 83 13 and and CC cord-016755-ye37z5h9 83 14 stained stain VBN cord-016755-ye37z5h9 83 15 with with IN cord-016755-ye37z5h9 83 16 fluorescein fluorescein NN cord-016755-ye37z5h9 83 17 labeled label VBN cord-016755-ye37z5h9 83 18 anti anti JJ cord-016755-ye37z5h9 83 19 - - JJ cord-016755-ye37z5h9 83 20 human human JJ cord-016755-ye37z5h9 83 21 IgG igg NN cord-016755-ye37z5h9 83 22 antibodies antibody NNS cord-016755-ye37z5h9 83 23 . . . cord-016755-ye37z5h9 84 1 ELISA ELISA NNP cord-016755-ye37z5h9 84 2 methods method NNS cord-016755-ye37z5h9 84 3 were be VBD cord-016755-ye37z5h9 84 4 developed develop VBN cord-016755-ye37z5h9 84 5 with with IN cord-016755-ye37z5h9 84 6 purified purify VBN cord-016755-ye37z5h9 84 7 recombinant recombinant JJ cord-016755-ye37z5h9 84 8 nucleocapsid nucleocapsid NN cord-016755-ye37z5h9 84 9 protein protein NN cord-016755-ye37z5h9 84 10 ( ( -LRB- cord-016755-ye37z5h9 84 11 NP NP NNP cord-016755-ye37z5h9 84 12 ) ) -RRB- cord-016755-ye37z5h9 84 13 of of IN cord-016755-ye37z5h9 84 14 the the DT cord-016755-ye37z5h9 84 15 virus virus NN cord-016755-ye37z5h9 84 16 produced produce VBN cord-016755-ye37z5h9 84 17 in in IN cord-016755-ye37z5h9 84 18 E. E. NNP cord-016755-ye37z5h9 84 19 coli coli NNS cord-016755-ye37z5h9 84 20 . . . cord-016755-ye37z5h9 85 1 Specificity specificity NN cord-016755-ye37z5h9 85 2 , , , cord-016755-ye37z5h9 85 3 sensitivity sensitivity NN cord-016755-ye37z5h9 85 4 and and CC cord-016755-ye37z5h9 85 5 cross cross NN cord-016755-ye37z5h9 85 6 reactivity reactivity NN cord-016755-ye37z5h9 85 7 of of IN cord-016755-ye37z5h9 85 8 the the DT cord-016755-ye37z5h9 85 9 methods method NNS cord-016755-ye37z5h9 85 10 were be VBD cord-016755-ye37z5h9 85 11 verified verify VBN cord-016755-ye37z5h9 85 12 with with IN cord-016755-ye37z5h9 85 13 serum serum NN cord-016755-ye37z5h9 85 14 samples sample NNS cord-016755-ye37z5h9 85 15 collected collect VBN cord-016755-ye37z5h9 85 16 from from IN cord-016755-ye37z5h9 85 17 patients patient NNS cord-016755-ye37z5h9 85 18 with with IN cord-016755-ye37z5h9 85 19 SFTS SFTS NNP cord-016755-ye37z5h9 85 20 confirmed confirm VBN cord-016755-ye37z5h9 85 21 by by IN cord-016755-ye37z5h9 85 22 RT RT NNP cord-016755-ye37z5h9 85 23 - - HYPH cord-016755-ye37z5h9 85 24 PCR PCR NNP cord-016755-ye37z5h9 85 25 and and CC cord-016755-ye37z5h9 85 26 sera sera NNP cord-016755-ye37z5h9 85 27 collected collect VBD cord-016755-ye37z5h9 85 28 from from IN cord-016755-ye37z5h9 85 29 healthy healthy JJ cord-016755-ye37z5h9 85 30 donors donor NNS cord-016755-ye37z5h9 85 31 from from IN cord-016755-ye37z5h9 85 32 the the DT cord-016755-ye37z5h9 85 33 areas area NNS cord-016755-ye37z5h9 85 34 without without IN cord-016755-ye37z5h9 85 35 reported report VBN cord-016755-ye37z5h9 85 36 SFTS SFTS NNP cord-016755-ye37z5h9 85 37 cases case NNS cord-016755-ye37z5h9 85 38 . . . cord-016755-ye37z5h9 86 1 For for IN cord-016755-ye37z5h9 86 2 micro micro JJ cord-016755-ye37z5h9 86 3 - - NN cord-016755-ye37z5h9 86 4 neutralization neutralization NN cord-016755-ye37z5h9 86 5 test test NN cord-016755-ye37z5h9 86 6 , , , cord-016755-ye37z5h9 86 7 sera sera NNP cord-016755-ye37z5h9 86 8 were be VBD cord-016755-ye37z5h9 86 9 mixed mix VBN cord-016755-ye37z5h9 86 10 with with IN cord-016755-ye37z5h9 86 11 an an DT cord-016755-ye37z5h9 86 12 equal equal JJ cord-016755-ye37z5h9 86 13 volume volume NN cord-016755-ye37z5h9 86 14 of of IN cord-016755-ye37z5h9 86 15 100 100 CD cord-016755-ye37z5h9 86 16 50 50 CD cord-016755-ye37z5h9 86 17 % % NN cord-016755-ye37z5h9 86 18 tissue tissue NN cord-016755-ye37z5h9 86 19 culture culture NN cord-016755-ye37z5h9 86 20 infectious infectious JJ cord-016755-ye37z5h9 86 21 dose dose NN cord-016755-ye37z5h9 86 22 ( ( -LRB- cord-016755-ye37z5h9 86 23 TCID TCID NNP cord-016755-ye37z5h9 86 24 50 50 CD cord-016755-ye37z5h9 86 25 ) ) -RRB- cord-016755-ye37z5h9 86 26 of of IN cord-016755-ye37z5h9 86 27 SFTS SFTS NNP cord-016755-ye37z5h9 86 28 virus virus NN cord-016755-ye37z5h9 86 29 ( ( -LRB- cord-016755-ye37z5h9 86 30 isolate isolate VB cord-016755-ye37z5h9 86 31 HB29 HB29 NNP cord-016755-ye37z5h9 86 32 ) ) -RRB- cord-016755-ye37z5h9 86 33 and and CC cord-016755-ye37z5h9 86 34 incubated incubate VBN cord-016755-ye37z5h9 86 35 at at IN cord-016755-ye37z5h9 86 36 37 37 CD cord-016755-ye37z5h9 86 37 ° ° NN cord-016755-ye37z5h9 86 38 C c NN cord-016755-ye37z5h9 86 39 for for IN cord-016755-ye37z5h9 86 40 1.5 1.5 CD cord-016755-ye37z5h9 86 41 h. h. NN cord-016755-ye37z5h9 87 1 The the DT cord-016755-ye37z5h9 87 2 mixture mixture NN cord-016755-ye37z5h9 87 3 ( ( -LRB- cord-016755-ye37z5h9 87 4 100 100 CD cord-016755-ye37z5h9 87 5 μl μl NN cord-016755-ye37z5h9 87 6 ) ) -RRB- cord-016755-ye37z5h9 87 7 was be VBD cord-016755-ye37z5h9 87 8 then then RB cord-016755-ye37z5h9 87 9 added add VBN cord-016755-ye37z5h9 87 10 to to IN cord-016755-ye37z5h9 87 11 a a DT cord-016755-ye37z5h9 87 12 96-well 96-well CD cord-016755-ye37z5h9 87 13 plate plate NN cord-016755-ye37z5h9 87 14 in in IN cord-016755-ye37z5h9 87 15 quadruplicates quadruplicate NNS cord-016755-ye37z5h9 87 16 . . . cord-016755-ye37z5h9 88 1 The the DT cord-016755-ye37z5h9 88 2 plates plate NNS cord-016755-ye37z5h9 88 3 were be VBD cord-016755-ye37z5h9 88 4 incubated incubate VBN cord-016755-ye37z5h9 88 5 in in IN cord-016755-ye37z5h9 88 6 5 5 CD cord-016755-ye37z5h9 88 7 % % NN cord-016755-ye37z5h9 88 8 CO co NN cord-016755-ye37z5h9 88 9 2 2 CD cord-016755-ye37z5h9 88 10 at at IN cord-016755-ye37z5h9 88 11 37 37 CD cord-016755-ye37z5h9 88 12 ° ° NN cord-016755-ye37z5h9 88 13 C c NN cord-016755-ye37z5h9 88 14 for for IN cord-016755-ye37z5h9 88 15 12 12 CD cord-016755-ye37z5h9 88 16 days day NNS cord-016755-ye37z5h9 88 17 . . . cord-016755-ye37z5h9 89 1 Viral viral JJ cord-016755-ye37z5h9 89 2 infection infection NN cord-016755-ye37z5h9 89 3 in in IN cord-016755-ye37z5h9 89 4 wells well NNS cord-016755-ye37z5h9 89 5 was be VBD cord-016755-ye37z5h9 89 6 detected detect VBN cord-016755-ye37z5h9 89 7 by by IN cord-016755-ye37z5h9 89 8 specific specific JJ cord-016755-ye37z5h9 89 9 IFA IFA NNP cord-016755-ye37z5h9 89 10 assays assay NNS cord-016755-ye37z5h9 89 11 with with IN cord-016755-ye37z5h9 89 12 a a DT cord-016755-ye37z5h9 89 13 confirmed confirm VBN cord-016755-ye37z5h9 89 14 patient patient JJ cord-016755-ye37z5h9 89 15 serum serum NN cord-016755-ye37z5h9 89 16 . . . cord-016755-ye37z5h9 90 1 The the DT cord-016755-ye37z5h9 90 2 endpoint endpoint NN cord-016755-ye37z5h9 90 3 titer titer NN cord-016755-ye37z5h9 90 4 was be VBD cord-016755-ye37z5h9 90 5 expressed express VBN cord-016755-ye37z5h9 90 6 as as IN cord-016755-ye37z5h9 90 7 the the DT cord-016755-ye37z5h9 90 8 reciprocal reciprocal NN cord-016755-ye37z5h9 90 9 of of IN cord-016755-ye37z5h9 90 10 the the DT cord-016755-ye37z5h9 90 11 highest high JJS cord-016755-ye37z5h9 90 12 dilution dilution NN cord-016755-ye37z5h9 90 13 of of IN cord-016755-ye37z5h9 90 14 serum serum NN cord-016755-ye37z5h9 90 15 . . . cord-016755-ye37z5h9 91 1 Viral Viral NNP cord-016755-ye37z5h9 91 2 RNA RNA NNP cord-016755-ye37z5h9 91 3 was be VBD cord-016755-ye37z5h9 91 4 detected detect VBN cord-016755-ye37z5h9 91 5 from from IN cord-016755-ye37z5h9 91 6 serum serum NN cord-016755-ye37z5h9 91 7 , , , cord-016755-ye37z5h9 91 8 whole whole JJ cord-016755-ye37z5h9 91 9 blood blood NN cord-016755-ye37z5h9 91 10 or or CC cord-016755-ye37z5h9 91 11 homogenized homogenize VBN cord-016755-ye37z5h9 91 12 arthropods arthropod NNS cord-016755-ye37z5h9 91 13 with with IN cord-016755-ye37z5h9 91 14 one one CD cord-016755-ye37z5h9 91 15 - - HYPH cord-016755-ye37z5h9 91 16 step step NN cord-016755-ye37z5h9 91 17 real real JJ cord-016755-ye37z5h9 91 18 - - HYPH cord-016755-ye37z5h9 91 19 time time NN cord-016755-ye37z5h9 91 20 RT RT NNP cord-016755-ye37z5h9 91 21 - - HYPH cord-016755-ye37z5h9 91 22 PCR PCR NNP cord-016755-ye37z5h9 91 23 assay assay NN cord-016755-ye37z5h9 91 24 using use VBG cord-016755-ye37z5h9 91 25 the the DT cord-016755-ye37z5h9 91 26 primers primer NNS cord-016755-ye37z5h9 91 27 and and CC cord-016755-ye37z5h9 91 28 probes probe NNS cord-016755-ye37z5h9 91 29 designed design VBN cord-016755-ye37z5h9 91 30 based base VBN cord-016755-ye37z5h9 91 31 on on IN cord-016755-ye37z5h9 91 32 the the DT cord-016755-ye37z5h9 91 33 3 3 CD cord-016755-ye37z5h9 91 34 viral viral JJ cord-016755-ye37z5h9 91 35 genome genome NN cord-016755-ye37z5h9 91 36 segments segment NNS cord-016755-ye37z5h9 91 37 . . . cord-016755-ye37z5h9 92 1 The the DT cord-016755-ye37z5h9 92 2 cutoff cutoff NN cord-016755-ye37z5h9 92 3 CT ct NN cord-016755-ye37z5h9 92 4 value value NN cord-016755-ye37z5h9 92 5 for for IN cord-016755-ye37z5h9 92 6 a a DT cord-016755-ye37z5h9 92 7 positive positive JJ cord-016755-ye37z5h9 92 8 sample sample NN cord-016755-ye37z5h9 92 9 was be VBD cord-016755-ye37z5h9 92 10 set set VBN cord-016755-ye37z5h9 92 11 at at IN cord-016755-ye37z5h9 92 12 35 35 CD cord-016755-ye37z5h9 92 13 cycles cycle NNS cord-016755-ye37z5h9 92 14 . . . cord-016755-ye37z5h9 93 1 Samples sample NNS cord-016755-ye37z5h9 93 2 from from IN cord-016755-ye37z5h9 93 3 which which WDT cord-016755-ye37z5h9 93 4 only only RB cord-016755-ye37z5h9 93 5 one one CD cord-016755-ye37z5h9 93 6 genomic genomic JJ cord-016755-ye37z5h9 93 7 segment segment NN cord-016755-ye37z5h9 93 8 was be VBD cord-016755-ye37z5h9 93 9 significantly significantly RB cord-016755-ye37z5h9 93 10 amplified amplify VBN cord-016755-ye37z5h9 93 11 were be VBD cord-016755-ye37z5h9 93 12 verified verify VBN cord-016755-ye37z5h9 93 13 by by IN cord-016755-ye37z5h9 93 14 nested nested JJ cord-016755-ye37z5h9 93 15 RT RT NNP cord-016755-ye37z5h9 93 16 - - HYPH cord-016755-ye37z5h9 93 17 PCR PCR NNP cord-016755-ye37z5h9 93 18 and and CC cord-016755-ye37z5h9 93 19 sequencing sequencing NN cord-016755-ye37z5h9 93 20 . . . cord-016755-ye37z5h9 94 1 SFTS SFTS NNP cord-016755-ye37z5h9 94 2 viral viral JJ cord-016755-ye37z5h9 94 3 RNA RNA NNP cord-016755-ye37z5h9 94 4 and/or and/or CC cord-016755-ye37z5h9 94 5 specific specific JJ cord-016755-ye37z5h9 94 6 antibodies antibody NNS cord-016755-ye37z5h9 94 7 were be VBD cord-016755-ye37z5h9 94 8 detected detect VBN cord-016755-ye37z5h9 94 9 in in IN cord-016755-ye37z5h9 94 10 171 171 CD cord-016755-ye37z5h9 94 11 out out IN cord-016755-ye37z5h9 94 12 of of IN cord-016755-ye37z5h9 94 13 241 241 CD cord-016755-ye37z5h9 94 14 hospitalized hospitalize VBN cord-016755-ye37z5h9 94 15 SFTS SFTS NNP cord-016755-ye37z5h9 94 16 patients patient NNS cord-016755-ye37z5h9 94 17 diagnosed diagnose VBD cord-016755-ye37z5h9 94 18 according accord VBG cord-016755-ye37z5h9 94 19 to to IN cord-016755-ye37z5h9 94 20 the the DT cord-016755-ye37z5h9 94 21 SFTS SFTS NNP cord-016755-ye37z5h9 94 22 case case NN cord-016755-ye37z5h9 94 23 definition definition NN cord-016755-ye37z5h9 94 24 ( ( -LRB- cord-016755-ye37z5h9 94 25 National National NNP cord-016755-ye37z5h9 94 26 2010 2010 CD cord-016755-ye37z5h9 94 27 ) ) -RRB- cord-016755-ye37z5h9 94 28 . . . cord-016755-ye37z5h9 95 1 These these DT cord-016755-ye37z5h9 95 2 patients patient NNS cord-016755-ye37z5h9 95 3 were be VBD cord-016755-ye37z5h9 95 4 found find VBN cord-016755-ye37z5h9 95 5 in in IN cord-016755-ye37z5h9 95 6 central central JJ cord-016755-ye37z5h9 95 7 and and CC cord-016755-ye37z5h9 95 8 north north JJ cord-016755-ye37z5h9 95 9 - - HYPH cord-016755-ye37z5h9 95 10 eastern eastern JJ cord-016755-ye37z5h9 95 11 areas area NNS cord-016755-ye37z5h9 95 12 of of IN cord-016755-ye37z5h9 95 13 China China NNP cord-016755-ye37z5h9 95 14 including include VBG cord-016755-ye37z5h9 95 15 of of IN cord-016755-ye37z5h9 95 16 Henan Henan NNP cord-016755-ye37z5h9 95 17 ( ( -LRB- cord-016755-ye37z5h9 95 18 43 43 CD cord-016755-ye37z5h9 95 19 cases case NNS cord-016755-ye37z5h9 95 20 ) ) -RRB- cord-016755-ye37z5h9 95 21 , , , cord-016755-ye37z5h9 95 22 Hubei Hubei NNP cord-016755-ye37z5h9 95 23 ( ( -LRB- cord-016755-ye37z5h9 95 24 52 52 CD cord-016755-ye37z5h9 95 25 cases case NNS cord-016755-ye37z5h9 95 26 ) ) -RRB- cord-016755-ye37z5h9 95 27 , , , cord-016755-ye37z5h9 95 28 Shandong Shandong NNP cord-016755-ye37z5h9 95 29 ( ( -LRB- cord-016755-ye37z5h9 95 30 93 93 CD cord-016755-ye37z5h9 95 31 cases case NNS cord-016755-ye37z5h9 95 32 ) ) -RRB- cord-016755-ye37z5h9 95 33 , , , cord-016755-ye37z5h9 95 34 Anhui Anhui NNP cord-016755-ye37z5h9 95 35 ( ( -LRB- cord-016755-ye37z5h9 95 36 31 31 CD cord-016755-ye37z5h9 95 37 cases case NNS cord-016755-ye37z5h9 95 38 ) ) -RRB- cord-016755-ye37z5h9 95 39 , , , cord-016755-ye37z5h9 95 40 Jiangsu Jiangsu NNP cord-016755-ye37z5h9 95 41 ( ( -LRB- cord-016755-ye37z5h9 95 42 11 11 CD cord-016755-ye37z5h9 95 43 cases case NNS cord-016755-ye37z5h9 95 44 ) ) -RRB- cord-016755-ye37z5h9 95 45 and and CC cord-016755-ye37z5h9 95 46 Liaoning Liaoning NNP cord-016755-ye37z5h9 95 47 Province Province NNP cord-016755-ye37z5h9 95 48 ( ( -LRB- cord-016755-ye37z5h9 95 49 11 11 CD cord-016755-ye37z5h9 95 50 cases case NNS cord-016755-ye37z5h9 95 51 ) ) -RRB- cord-016755-ye37z5h9 95 52 . . . cord-016755-ye37z5h9 96 1 But but CC cord-016755-ye37z5h9 96 2 viral viral JJ cord-016755-ye37z5h9 96 3 RNA RNA NNP cord-016755-ye37z5h9 96 4 and and CC cord-016755-ye37z5h9 96 5 specific specific JJ cord-016755-ye37z5h9 96 6 antibodies antibody NNS cord-016755-ye37z5h9 96 7 were be VBD cord-016755-ye37z5h9 96 8 not not RB cord-016755-ye37z5h9 96 9 detected detect VBN cord-016755-ye37z5h9 96 10 from from IN cord-016755-ye37z5h9 96 11 the the DT cord-016755-ye37z5h9 96 12 sera sera NNP cord-016755-ye37z5h9 96 13 collected collect VBD cord-016755-ye37z5h9 96 14 from from IN cord-016755-ye37z5h9 96 15 200 200 CD cord-016755-ye37z5h9 96 16 patient patient NN cord-016755-ye37z5h9 96 17 - - HYPH cord-016755-ye37z5h9 96 18 matched match VBN cord-016755-ye37z5h9 96 19 healthy healthy JJ cord-016755-ye37z5h9 96 20 donors donor NNS cord-016755-ye37z5h9 96 21 in in IN cord-016755-ye37z5h9 96 22 the the DT cord-016755-ye37z5h9 96 23 endemic endemic JJ cord-016755-ye37z5h9 96 24 areas area NNS cord-016755-ye37z5h9 96 25 , , , cord-016755-ye37z5h9 96 26 180 180 CD cord-016755-ye37z5h9 96 27 healthy healthy JJ cord-016755-ye37z5h9 96 28 donors donor NNS cord-016755-ye37z5h9 96 29 from from IN cord-016755-ye37z5h9 96 30 non non JJ cord-016755-ye37z5h9 96 31 - - JJ cord-016755-ye37z5h9 96 32 endemic endemic JJ cord-016755-ye37z5h9 96 33 area area NN cord-016755-ye37z5h9 96 34 , , , cord-016755-ye37z5h9 96 35 and and CC cord-016755-ye37z5h9 96 36 54 54 CD cord-016755-ye37z5h9 96 37 HFRS HFRS NNP cord-016755-ye37z5h9 96 38 suspected suspect VBN cord-016755-ye37z5h9 96 39 cases case NNS cord-016755-ye37z5h9 96 40 . . . cord-016755-ye37z5h9 97 1 Viral Viral NNP cord-016755-ye37z5h9 97 2 RNA RNA NNP cord-016755-ye37z5h9 97 3 was be VBD cord-016755-ye37z5h9 97 4 not not RB cord-016755-ye37z5h9 97 5 found find VBN cord-016755-ye37z5h9 97 6 in in IN cord-016755-ye37z5h9 97 7 any any DT cord-016755-ye37z5h9 97 8 of of IN cord-016755-ye37z5h9 97 9 5900 5900 CD cord-016755-ye37z5h9 97 10 mosquitoes mosquito NNS cord-016755-ye37z5h9 97 11 captured capture VBN cord-016755-ye37z5h9 97 12 from from IN cord-016755-ye37z5h9 97 13 the the DT cord-016755-ye37z5h9 97 14 areas area NNS cord-016755-ye37z5h9 97 15 with with IN cord-016755-ye37z5h9 97 16 SFTS SFTS NNP cord-016755-ye37z5h9 97 17 patients patient NNS cord-016755-ye37z5h9 97 18 living live VBG cord-016755-ye37z5h9 97 19 . . . cord-016755-ye37z5h9 98 1 On on IN cord-016755-ye37z5h9 98 2 the the DT cord-016755-ye37z5h9 98 3 other other JJ cord-016755-ye37z5h9 98 4 hand hand NN cord-016755-ye37z5h9 98 5 , , , cord-016755-ye37z5h9 98 6 10 10 CD cord-016755-ye37z5h9 98 7 out out IN cord-016755-ye37z5h9 98 8 of of IN cord-016755-ye37z5h9 98 9 186 186 CD cord-016755-ye37z5h9 98 10 ticks tick NNS cord-016755-ye37z5h9 98 11 ( ( -LRB- cord-016755-ye37z5h9 98 12 5.4 5.4 CD cord-016755-ye37z5h9 98 13 % % NN cord-016755-ye37z5h9 98 14 ) ) -RRB- cord-016755-ye37z5h9 98 15 of of IN cord-016755-ye37z5h9 98 16 the the DT cord-016755-ye37z5h9 98 17 species specie NNS cord-016755-ye37z5h9 98 18 Haemaphysalis Haemaphysalis NNP cord-016755-ye37z5h9 98 19 longicornis longicornis NNP cord-016755-ye37z5h9 98 20 collected collect VBD cord-016755-ye37z5h9 98 21 from from IN cord-016755-ye37z5h9 98 22 domestic domestic JJ cord-016755-ye37z5h9 98 23 animals animal NNS cord-016755-ye37z5h9 98 24 in in IN cord-016755-ye37z5h9 98 25 the the DT cord-016755-ye37z5h9 98 26 same same JJ cord-016755-ye37z5h9 98 27 areas area NNS cord-016755-ye37z5h9 98 28 were be VBD cord-016755-ye37z5h9 98 29 positive positive JJ cord-016755-ye37z5h9 98 30 for for IN cord-016755-ye37z5h9 98 31 SFTS SFTS NNP cord-016755-ye37z5h9 98 32 virus virus NN cord-016755-ye37z5h9 98 33 RNA RNA NNP cord-016755-ye37z5h9 98 34 . . . cord-016755-ye37z5h9 99 1 To to TO cord-016755-ye37z5h9 99 2 explore explore VB cord-016755-ye37z5h9 99 3 the the DT cord-016755-ye37z5h9 99 4 seroconversion seroconversion NN cord-016755-ye37z5h9 99 5 against against IN cord-016755-ye37z5h9 99 6 SFTS SFTS NNP cord-016755-ye37z5h9 99 7 virus virus NN cord-016755-ye37z5h9 99 8 in in IN cord-016755-ye37z5h9 99 9 patients patient NNS cord-016755-ye37z5h9 99 10 with with IN cord-016755-ye37z5h9 99 11 SFTS SFTS NNP cord-016755-ye37z5h9 99 12 , , , cord-016755-ye37z5h9 99 13 a a DT cord-016755-ye37z5h9 99 14 cohort cohort NN cord-016755-ye37z5h9 99 15 of of IN cord-016755-ye37z5h9 99 16 35 35 CD cord-016755-ye37z5h9 99 17 RT RT NNP cord-016755-ye37z5h9 99 18 - - HYPH cord-016755-ye37z5h9 99 19 PCR PCR NNP cord-016755-ye37z5h9 99 20 - - HYPH cord-016755-ye37z5h9 99 21 confirmed confirm VBN cord-016755-ye37z5h9 99 22 patients patient NNS cord-016755-ye37z5h9 99 23 was be VBD cord-016755-ye37z5h9 99 24 selected select VBN cord-016755-ye37z5h9 99 25 , , , cord-016755-ye37z5h9 99 26 sera sera NNP cord-016755-ye37z5h9 99 27 at at IN cord-016755-ye37z5h9 99 28 both both DT cord-016755-ye37z5h9 99 29 acute acute JJ cord-016755-ye37z5h9 99 30 - - : cord-016755-ye37z5h9 99 31 and and CC cord-016755-ye37z5h9 99 32 convalescentphase convalescentphase NN cord-016755-ye37z5h9 99 33 were be VBD cord-016755-ye37z5h9 99 34 collected collect VBN cord-016755-ye37z5h9 99 35 . . . cord-016755-ye37z5h9 100 1 Specific specific JJ cord-016755-ye37z5h9 100 2 antibodies antibody NNS cord-016755-ye37z5h9 100 3 were be VBD cord-016755-ye37z5h9 100 4 quantified quantify VBN cord-016755-ye37z5h9 100 5 using use VBG cord-016755-ye37z5h9 100 6 IFA IFA NNP cord-016755-ye37z5h9 100 7 , , , cord-016755-ye37z5h9 100 8 ELISA ELISA NNP cord-016755-ye37z5h9 100 9 and and CC cord-016755-ye37z5h9 100 10 micro micro JJ cord-016755-ye37z5h9 100 11 - - NN cord-016755-ye37z5h9 100 12 neutralization neutralization NN cord-016755-ye37z5h9 100 13 test test NN cord-016755-ye37z5h9 100 14 . . . cord-016755-ye37z5h9 101 1 Four four CD cord-016755-ye37z5h9 101 2 fold fold JJ cord-016755-ye37z5h9 101 3 elevation elevation NN cord-016755-ye37z5h9 101 4 of of IN cord-016755-ye37z5h9 101 5 antibody antibody NN cord-016755-ye37z5h9 101 6 titers titer NNS cord-016755-ye37z5h9 101 7 or or CC cord-016755-ye37z5h9 101 8 seroconversion seroconversion NN cord-016755-ye37z5h9 101 9 from from IN cord-016755-ye37z5h9 101 10 negative negative JJ cord-016755-ye37z5h9 101 11 to to IN cord-016755-ye37z5h9 101 12 positive positive JJ cord-016755-ye37z5h9 101 13 reactivity reactivity NN cord-016755-ye37z5h9 101 14 in in IN cord-016755-ye37z5h9 101 15 serological serological JJ cord-016755-ye37z5h9 101 16 tests test NNS cord-016755-ye37z5h9 101 17 were be VBD cord-016755-ye37z5h9 101 18 found find VBN cord-016755-ye37z5h9 101 19 in in IN cord-016755-ye37z5h9 101 20 all all DT cord-016755-ye37z5h9 101 21 of of IN cord-016755-ye37z5h9 101 22 the the DT cord-016755-ye37z5h9 101 23 35 35 CD cord-016755-ye37z5h9 101 24 patients patient NNS cord-016755-ye37z5h9 101 25 . . . cord-016755-ye37z5h9 102 1 This this DT cord-016755-ye37z5h9 102 2 indicated indicate VBD cord-016755-ye37z5h9 102 3 that that IN cord-016755-ye37z5h9 102 4 high high JJ cord-016755-ye37z5h9 102 5 - - HYPH cord-016755-ye37z5h9 102 6 titer titer NN cord-016755-ye37z5h9 102 7 neutralizing neutralize VBG cord-016755-ye37z5h9 102 8 antibodies antibody NNS cord-016755-ye37z5h9 102 9 were be VBD cord-016755-ye37z5h9 102 10 generated generate VBN cord-016755-ye37z5h9 102 11 during during IN cord-016755-ye37z5h9 102 12 the the DT cord-016755-ye37z5h9 102 13 convalescent convalescent JJ cord-016755-ye37z5h9 102 14 phase phase NN cord-016755-ye37z5h9 102 15 of of IN cord-016755-ye37z5h9 102 16 the the DT cord-016755-ye37z5h9 102 17 disease disease NN cord-016755-ye37z5h9 102 18 . . . cord-016755-ye37z5h9 103 1 An an DT cord-016755-ye37z5h9 103 2 antibody antibody NN cord-016755-ye37z5h9 103 3 titer titer NN cord-016755-ye37z5h9 103 4 of of IN cord-016755-ye37z5h9 103 5 more more JJR cord-016755-ye37z5h9 103 6 than than IN cord-016755-ye37z5h9 103 7 25600 25600 CD cord-016755-ye37z5h9 103 8 in in IN cord-016755-ye37z5h9 103 9 ELISA ELISA NNP cord-016755-ye37z5h9 103 10 was be VBD cord-016755-ye37z5h9 103 11 found find VBN cord-016755-ye37z5h9 103 12 in in IN cord-016755-ye37z5h9 103 13 sera sera NNP cord-016755-ye37z5h9 103 14 from from IN cord-016755-ye37z5h9 103 15 15 15 CD cord-016755-ye37z5h9 103 16 patients patient NNS cord-016755-ye37z5h9 103 17 at at IN cord-016755-ye37z5h9 103 18 convalescent convalescent JJ cord-016755-ye37z5h9 103 19 phase phase NN cord-016755-ye37z5h9 103 20 , , , cord-016755-ye37z5h9 103 21 indicating indicate VBG cord-016755-ye37z5h9 103 22 a a DT cord-016755-ye37z5h9 103 23 robust robust JJ cord-016755-ye37z5h9 103 24 humoral humoral JJ cord-016755-ye37z5h9 103 25 immune immune JJ cord-016755-ye37z5h9 103 26 response response NN cord-016755-ye37z5h9 103 27 against against IN cord-016755-ye37z5h9 103 28 SFTS SFTS NNP cord-016755-ye37z5h9 103 29 virus virus NN cord-016755-ye37z5h9 103 30 . . . cord-016755-ye37z5h9 104 1 The the DT cord-016755-ye37z5h9 104 2 discovery discovery NN cord-016755-ye37z5h9 104 3 of of IN cord-016755-ye37z5h9 104 4 the the DT cord-016755-ye37z5h9 104 5 new new JJ cord-016755-ye37z5h9 104 6 SFTS SFTS NNP cord-016755-ye37z5h9 104 7 virus virus NN cord-016755-ye37z5h9 104 8 was be VBD cord-016755-ye37z5h9 104 9 the the DT cord-016755-ye37z5h9 104 10 result result NN cord-016755-ye37z5h9 104 11 of of IN cord-016755-ye37z5h9 104 12 the the DT cord-016755-ye37z5h9 104 13 joint joint JJ cord-016755-ye37z5h9 104 14 efforts effort NNS cord-016755-ye37z5h9 104 15 of of IN cord-016755-ye37z5h9 104 16 virologists virologist NNS cord-016755-ye37z5h9 104 17 , , , cord-016755-ye37z5h9 104 18 clinicians clinician NNS cord-016755-ye37z5h9 104 19 and and CC cord-016755-ye37z5h9 104 20 epidemiologists epidemiologist NNS cord-016755-ye37z5h9 104 21 , , , cord-016755-ye37z5h9 104 22 and and CC cord-016755-ye37z5h9 104 23 also also RB cord-016755-ye37z5h9 104 24 depends depend VBZ cord-016755-ye37z5h9 104 25 on on IN cord-016755-ye37z5h9 104 26 the the DT cord-016755-ye37z5h9 104 27 progress progress NN cord-016755-ye37z5h9 104 28 of of IN cord-016755-ye37z5h9 104 29 laboratory laboratory NN cord-016755-ye37z5h9 104 30 testing testing NN cord-016755-ye37z5h9 104 31 technology technology NN cord-016755-ye37z5h9 104 32 . . . cord-016755-ye37z5h9 105 1 Robust robust JJ cord-016755-ye37z5h9 105 2 epidemiological epidemiological JJ cord-016755-ye37z5h9 105 3 , , , cord-016755-ye37z5h9 105 4 clinical clinical JJ cord-016755-ye37z5h9 105 5 , , , cord-016755-ye37z5h9 105 6 serological serological JJ cord-016755-ye37z5h9 105 7 and and CC cord-016755-ye37z5h9 105 8 virological virological JJ cord-016755-ye37z5h9 105 9 data datum NNS cord-016755-ye37z5h9 105 10 had have VBD cord-016755-ye37z5h9 105 11 been be VBN cord-016755-ye37z5h9 105 12 collected collect VBN cord-016755-ye37z5h9 105 13 to to TO cord-016755-ye37z5h9 105 14 support support VB cord-016755-ye37z5h9 105 15 that that IN cord-016755-ye37z5h9 105 16 SFTS SFTS NNP cord-016755-ye37z5h9 105 17 virus virus NN cord-016755-ye37z5h9 105 18 is be VBZ cord-016755-ye37z5h9 105 19 the the DT cord-016755-ye37z5h9 105 20 cause cause NN cord-016755-ye37z5h9 105 21 of of IN cord-016755-ye37z5h9 105 22 the the DT cord-016755-ye37z5h9 105 23 SFTS SFTS NNP cord-016755-ye37z5h9 105 24 disease disease NN cord-016755-ye37z5h9 105 25 identified identify VBN cord-016755-ye37z5h9 105 26 in in IN cord-016755-ye37z5h9 105 27 central central JJ cord-016755-ye37z5h9 105 28 - - HYPH cord-016755-ye37z5h9 105 29 eastern eastern JJ cord-016755-ye37z5h9 105 30 and and CC cord-016755-ye37z5h9 105 31 northeastern northeastern JJ cord-016755-ye37z5h9 105 32 parts part NNS cord-016755-ye37z5h9 105 33 of of IN cord-016755-ye37z5h9 105 34 China China NNP cord-016755-ye37z5h9 105 35 . . . cord-016755-ye37z5h9 106 1 Of of RB cord-016755-ye37z5h9 106 2 course course RB cord-016755-ye37z5h9 106 3 , , , cord-016755-ye37z5h9 106 4 SFTS SFTS NNP cord-016755-ye37z5h9 106 5 is be VBZ cord-016755-ye37z5h9 106 6 not not RB cord-016755-ye37z5h9 106 7 a a DT cord-016755-ye37z5h9 106 8 disease disease NN cord-016755-ye37z5h9 106 9 with with IN cord-016755-ye37z5h9 106 10 a a DT cord-016755-ye37z5h9 106 11 single single JJ cord-016755-ye37z5h9 106 12 cause cause NN cord-016755-ye37z5h9 106 13 , , , cord-016755-ye37z5h9 106 14 in in IN cord-016755-ye37z5h9 106 15 addition addition NN cord-016755-ye37z5h9 106 16 to to IN cord-016755-ye37z5h9 106 17 non non JJ cord-016755-ye37z5h9 106 18 - - JJ cord-016755-ye37z5h9 106 19 infectious infectious JJ cord-016755-ye37z5h9 106 20 diseases disease NNS cord-016755-ye37z5h9 106 21 , , , cord-016755-ye37z5h9 106 22 a a DT cord-016755-ye37z5h9 106 23 variety variety NN cord-016755-ye37z5h9 106 24 of of IN cord-016755-ye37z5h9 106 25 known know VBN cord-016755-ye37z5h9 106 26 microbial microbial JJ cord-016755-ye37z5h9 106 27 pathogens pathogen NNS cord-016755-ye37z5h9 106 28 can can MD cord-016755-ye37z5h9 106 29 cause cause VB cord-016755-ye37z5h9 106 30 SFTS SFTS NNP cord-016755-ye37z5h9 106 31 , , , cord-016755-ye37z5h9 106 32 which which WDT cord-016755-ye37z5h9 106 33 needs need VBZ cord-016755-ye37z5h9 106 34 to to TO cord-016755-ye37z5h9 106 35 be be VB cord-016755-ye37z5h9 106 36 differentiated differentiate VBN cord-016755-ye37z5h9 106 37 from from IN cord-016755-ye37z5h9 106 38 the the DT cord-016755-ye37z5h9 106 39 other other JJ cord-016755-ye37z5h9 106 40 related related JJ cord-016755-ye37z5h9 106 41 pathogens pathogen NNS cord-016755-ye37z5h9 106 42 . . . cord-016755-ye37z5h9 107 1 A a DT cord-016755-ye37z5h9 107 2 virus virus NN cord-016755-ye37z5h9 107 3 discovery discovery NN cord-016755-ye37z5h9 107 4 method method NN cord-016755-ye37z5h9 107 5 incorporating incorporate VBG cord-016755-ye37z5h9 107 6 DNase DNase NNP cord-016755-ye37z5h9 107 7 treatment treatment NN cord-016755-ye37z5h9 107 8 and and CC cord-016755-ye37z5h9 107 9 its -PRON- PRP$ cord-016755-ye37z5h9 107 10 application application NN cord-016755-ye37z5h9 107 11 to to IN cord-016755-ye37z5h9 107 12 the the DT cord-016755-ye37z5h9 107 13 identification identification NN cord-016755-ye37z5h9 107 14 of of IN cord-016755-ye37z5h9 107 15 two two CD cord-016755-ye37z5h9 107 16 bovine bovine JJ cord-016755-ye37z5h9 107 17 parvovirus parvovirus NN cord-016755-ye37z5h9 107 18 species specie NNS cord-016755-ye37z5h9 107 19 Virus Virus NNP cord-016755-ye37z5h9 107 20 discovery discovery NN cord-016755-ye37z5h9 107 21 by by IN cord-016755-ye37z5h9 107 22 sequence sequence NN cord-016755-ye37z5h9 107 23 - - HYPH cord-016755-ye37z5h9 107 24 independent independent JJ cord-016755-ye37z5h9 107 25 genome genome NN cord-016755-ye37z5h9 107 26 amplification amplification NN cord-016755-ye37z5h9 107 27 Induction Induction NNP cord-016755-ye37z5h9 107 28 of of IN cord-016755-ye37z5h9 107 29 severe severe JJ cord-016755-ye37z5h9 107 30 disease disease NN cord-016755-ye37z5h9 107 31 in in IN cord-016755-ye37z5h9 107 32 hamsters hamster NNS cord-016755-ye37z5h9 107 33 by by IN cord-016755-ye37z5h9 107 34 two two CD cord-016755-ye37z5h9 107 35 sandfly sandfly NNP cord-016755-ye37z5h9 107 36 fever fever NN cord-016755-ye37z5h9 107 37 group group NN cord-016755-ye37z5h9 107 38 viruses virus NNS cord-016755-ye37z5h9 107 39 , , , cord-016755-ye37z5h9 107 40 Punta Punta NNP cord-016755-ye37z5h9 107 41 Toro Toro NNP cord-016755-ye37z5h9 107 42 and and CC cord-016755-ye37z5h9 107 43 Gabek Gabek NNP cord-016755-ye37z5h9 107 44 Forest Forest NNP cord-016755-ye37z5h9 107 45 ( ( -LRB- cord-016755-ye37z5h9 107 46 Phlebovirus Phlebovirus NNP cord-016755-ye37z5h9 107 47 , , , cord-016755-ye37z5h9 107 48 Bunyaviridae Bunyaviridae NNP cord-016755-ye37z5h9 107 49 ) ) -RRB- cord-016755-ye37z5h9 107 50 , , , cord-016755-ye37z5h9 107 51 similar similar JJ cord-016755-ye37z5h9 107 52 to to IN cord-016755-ye37z5h9 107 53 that that DT cord-016755-ye37z5h9 107 54 caused cause VBN cord-016755-ye37z5h9 107 55 by by IN cord-016755-ye37z5h9 107 56 Rift Rift NNP cord-016755-ye37z5h9 107 57 Valley Valley NNP cord-016755-ye37z5h9 107 58 fever fever NN cord-016755-ye37z5h9 107 59 virus virus NN cord-016755-ye37z5h9 107 60 Guideline Guideline NNP cord-016755-ye37z5h9 107 61 for for IN cord-016755-ye37z5h9 107 62 prevention prevention NN cord-016755-ye37z5h9 107 63 and and CC cord-016755-ye37z5h9 107 64 control control NN cord-016755-ye37z5h9 107 65 of of IN cord-016755-ye37z5h9 107 66 severe severe JJ cord-016755-ye37z5h9 107 67 fever fever NN cord-016755-ye37z5h9 107 68 with with IN cord-016755-ye37z5h9 107 69 thrombocytopenic thrombocytopenic JJ cord-016755-ye37z5h9 107 70 syndrome syndrome NN cord-016755-ye37z5h9 107 71 Rapid Rapid NNP cord-016755-ye37z5h9 107 72 Identification Identification NNP cord-016755-ye37z5h9 107 73 of of IN cord-016755-ye37z5h9 107 74 Known Known NNP cord-016755-ye37z5h9 107 75 and and CC cord-016755-ye37z5h9 107 76 New New NNP cord-016755-ye37z5h9 107 77 RNA RNA NNP cord-016755-ye37z5h9 107 78 Viruses Viruses NNPS cord-016755-ye37z5h9 107 79 from from IN cord-016755-ye37z5h9 107 80 Animal Animal NNP cord-016755-ye37z5h9 107 81 Tissues tissue NNS cord-016755-ye37z5h9 107 82 Fever Fever NNP cord-016755-ye37z5h9 107 83 with with IN cord-016755-ye37z5h9 107 84 thrombocytopenia thrombocytopenia NN cord-016755-ye37z5h9 107 85 associated associate VBN cord-016755-ye37z5h9 107 86 with with IN cord-016755-ye37z5h9 107 87 a a DT cord-016755-ye37z5h9 107 88 novel novel NN cord-016755-ye37z5h9 107 89 bunyavirus bunyavirus NN cord-016755-ye37z5h9 107 90 in in IN cord-016755-ye37z5h9 107 91 China China NNP cord-016755-ye37z5h9 107 92 Nosocomial Nosocomial NNP cord-016755-ye37z5h9 107 93 transmission transmission NN cord-016755-ye37z5h9 107 94 of of IN cord-016755-ye37z5h9 107 95 human human JJ cord-016755-ye37z5h9 107 96 granulocytic granulocytic JJ cord-016755-ye37z5h9 107 97 anaplasmosis anaplasmosis NN cord-016755-ye37z5h9 107 98 in in IN cord-016755-ye37z5h9 107 99 China China NNP