id	sid	tid	token	lemma	pos
cord-271701-tx0lqgff	1	1	key	key	NN
cord-271701-tx0lqgff	1	2	:	:	:
cord-271701-tx0lqgff	2	1	cord-271701-tx0lqgff	cord-271701-tx0lqgff	NFP
cord-271701-tx0lqgff	2	2	authors	author	NNS
cord-271701-tx0lqgff	2	3	:	:	:
cord-271701-tx0lqgff	2	4	te	te	NNP
cord-271701-tx0lqgff	2	5	Velthuis	Velthuis	NNP
cord-271701-tx0lqgff	2	6	,	,	,
cord-271701-tx0lqgff	2	7	Aartjan	Aartjan	NNP
cord-271701-tx0lqgff	2	8	J.W.	J.W.	NNP
cord-271701-tx0lqgff	2	9	;	;	:
cord-271701-tx0lqgff	2	10	van	van	NNP
cord-271701-tx0lqgff	2	11	den	den	NNP
cord-271701-tx0lqgff	2	12	Worm	Worm	NNP
cord-271701-tx0lqgff	2	13	,	,	,
cord-271701-tx0lqgff	2	14	Sjoerd	Sjoerd	NNP
cord-271701-tx0lqgff	2	15	H.	H.	NNP
cord-271701-tx0lqgff	2	16	E.	E.	NNP
cord-271701-tx0lqgff	2	17	;	;	:
cord-271701-tx0lqgff	2	18	Snijder	Snijder	NNP
cord-271701-tx0lqgff	2	19	,	,	,
cord-271701-tx0lqgff	2	20	Eric	Eric	NNP
cord-271701-tx0lqgff	2	21	J.	J.	NNP
cord-271701-tx0lqgff	2	22	title	title	NN
cord-271701-tx0lqgff	2	23	:	:	:
cord-271701-tx0lqgff	3	1	The	the	DT
cord-271701-tx0lqgff	3	2	SARS	SARS	NNP
cord-271701-tx0lqgff	3	3	-	-	HYPH
cord-271701-tx0lqgff	3	4	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	3	5	nsp7+nsp8	nsp7+nsp8	NNP
cord-271701-tx0lqgff	3	6	complex	complex	NN
cord-271701-tx0lqgff	3	7	is	be	VBZ
cord-271701-tx0lqgff	3	8	a	a	DT
cord-271701-tx0lqgff	3	9	unique	unique	JJ
cord-271701-tx0lqgff	3	10	multimeric	multimeric	JJ
cord-271701-tx0lqgff	3	11	RNA	RNA	NNP
cord-271701-tx0lqgff	3	12	polymerase	polymerase	NN
cord-271701-tx0lqgff	3	13	capable	capable	JJ
cord-271701-tx0lqgff	3	14	of	of	IN
cord-271701-tx0lqgff	3	15	both	both	DT
cord-271701-tx0lqgff	3	16	de	de	FW
cord-271701-tx0lqgff	3	17	novo	novo	NNP
cord-271701-tx0lqgff	3	18	initiation	initiation	NN
cord-271701-tx0lqgff	3	19	and	and	CC
cord-271701-tx0lqgff	3	20	primer	primer	NN
cord-271701-tx0lqgff	3	21	extension	extension	NN
cord-271701-tx0lqgff	3	22	date	date	NN
cord-271701-tx0lqgff	3	23	:	:	:
cord-271701-tx0lqgff	3	24	2011	2011	CD
cord-271701-tx0lqgff	3	25	-	-	SYM
cord-271701-tx0lqgff	3	26	10	10	CD
cord-271701-tx0lqgff	3	27	-	-	SYM
cord-271701-tx0lqgff	3	28	29	29	CD
cord-271701-tx0lqgff	3	29	journal	journal	NN
cord-271701-tx0lqgff	3	30	:	:	:
cord-271701-tx0lqgff	3	31	Nucleic	nucleic	JJ
cord-271701-tx0lqgff	3	32	Acids	acid	NNS
cord-271701-tx0lqgff	3	33	Res	res	NN
cord-271701-tx0lqgff	3	34	DOI	doi	NN
cord-271701-tx0lqgff	3	35	:	:	:
cord-271701-tx0lqgff	3	36	10.1093	10.1093	CD
cord-271701-tx0lqgff	3	37	/	/	SYM
cord-271701-tx0lqgff	3	38	nar	nar	NNP
cord-271701-tx0lqgff	3	39	/	/	SYM
cord-271701-tx0lqgff	3	40	gkr893	gkr893	NNP
cord-271701-tx0lqgff	3	41	sha	sha	NNP
cord-271701-tx0lqgff	3	42	:	:	:
cord-271701-tx0lqgff	4	1	24df3883c547f5976077bc7a924eec9ae8fa9e71	24df3883c547f5976077bc7a924eec9ae8fa9e71	CD
cord-271701-tx0lqgff	4	2	doc_id	doc_id	CD
cord-271701-tx0lqgff	4	3	:	:	:
cord-271701-tx0lqgff	4	4	271701	271701	CD
cord-271701-tx0lqgff	4	5	cord_uid	cord_uid	NNS
cord-271701-tx0lqgff	4	6	:	:	:
cord-271701-tx0lqgff	4	7	tx0lqgff	tx0lqgff	.
cord-271701-tx0lqgff	5	1	Uniquely	uniquely	RB
cord-271701-tx0lqgff	5	2	among	among	IN
cord-271701-tx0lqgff	5	3	RNA	RNA	NNP
cord-271701-tx0lqgff	5	4	viruses	virus	NNS
cord-271701-tx0lqgff	5	5	,	,	,
cord-271701-tx0lqgff	5	6	replication	replication	NN
cord-271701-tx0lqgff	5	7	of	of	IN
cord-271701-tx0lqgff	5	8	the	the	DT
cord-271701-tx0lqgff	5	9	∼30-kb	∼30-kb	NNP
cord-271701-tx0lqgff	5	10	SARS	SARS	NNP
cord-271701-tx0lqgff	5	11	-	-	HYPH
cord-271701-tx0lqgff	5	12	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	5	13	genome	genome	NN
cord-271701-tx0lqgff	5	14	is	be	VBZ
cord-271701-tx0lqgff	5	15	believed	believe	VBN
cord-271701-tx0lqgff	5	16	to	to	TO
cord-271701-tx0lqgff	5	17	involve	involve	VB
cord-271701-tx0lqgff	5	18	two	two	CD
cord-271701-tx0lqgff	5	19	RNA	RNA	NNP
cord-271701-tx0lqgff	5	20	-	-	HYPH
cord-271701-tx0lqgff	5	21	dependent	dependent	JJ
cord-271701-tx0lqgff	5	22	RNA	RNA	NNP
cord-271701-tx0lqgff	5	23	polymerase	polymerase	NN
cord-271701-tx0lqgff	5	24	(	(	-LRB-
cord-271701-tx0lqgff	5	25	RdRp	RdRp	NNP
cord-271701-tx0lqgff	5	26	)	)	-RRB-
cord-271701-tx0lqgff	5	27	activities	activity	NNS
cord-271701-tx0lqgff	5	28	.	.	.
cord-271701-tx0lqgff	6	1	The	the	DT
cord-271701-tx0lqgff	6	2	first	first	JJ
cord-271701-tx0lqgff	6	3	is	be	VBZ
cord-271701-tx0lqgff	6	4	primer	primer	NN
cord-271701-tx0lqgff	6	5	-	-	HYPH
cord-271701-tx0lqgff	6	6	dependent	dependent	JJ
cord-271701-tx0lqgff	6	7	and	and	CC
cord-271701-tx0lqgff	6	8	associated	associate	VBN
cord-271701-tx0lqgff	6	9	with	with	IN
cord-271701-tx0lqgff	6	10	the	the	DT
cord-271701-tx0lqgff	6	11	106-kDa	106-kda	CD
cord-271701-tx0lqgff	6	12	non	non	JJ
cord-271701-tx0lqgff	6	13	-	-	JJ
cord-271701-tx0lqgff	6	14	structural	structural	JJ
cord-271701-tx0lqgff	6	15	protein	protein	NN
cord-271701-tx0lqgff	6	16	12	12	CD
cord-271701-tx0lqgff	6	17	(	(	-LRB-
cord-271701-tx0lqgff	6	18	nsp12	nsp12	CD
cord-271701-tx0lqgff	6	19	)	)	-RRB-
cord-271701-tx0lqgff	6	20	,	,	,
cord-271701-tx0lqgff	6	21	whereas	whereas	IN
cord-271701-tx0lqgff	6	22	the	the	DT
cord-271701-tx0lqgff	6	23	second	second	JJ
cord-271701-tx0lqgff	6	24	is	be	VBZ
cord-271701-tx0lqgff	6	25	catalysed	catalyse	VBN
cord-271701-tx0lqgff	6	26	by	by	IN
cord-271701-tx0lqgff	6	27	the	the	DT
cord-271701-tx0lqgff	6	28	22-kDa	22-kda	CD
cord-271701-tx0lqgff	6	29	nsp8	nsp8	NN
cord-271701-tx0lqgff	6	30	.	.	.
cord-271701-tx0lqgff	7	1	This	this	DT
cord-271701-tx0lqgff	7	2	latter	latter	JJ
cord-271701-tx0lqgff	7	3	enzyme	enzyme	NN
cord-271701-tx0lqgff	7	4	is	be	VBZ
cord-271701-tx0lqgff	7	5	capable	capable	JJ
cord-271701-tx0lqgff	7	6	of	of	IN
cord-271701-tx0lqgff	7	7	de	de	NNP
cord-271701-tx0lqgff	7	8	novo	novo	NNP
cord-271701-tx0lqgff	7	9	initiation	initiation	NN
cord-271701-tx0lqgff	7	10	and	and	CC
cord-271701-tx0lqgff	7	11	has	have	VBZ
cord-271701-tx0lqgff	7	12	been	be	VBN
cord-271701-tx0lqgff	7	13	proposed	propose	VBN
cord-271701-tx0lqgff	7	14	to	to	TO
cord-271701-tx0lqgff	7	15	operate	operate	VB
cord-271701-tx0lqgff	7	16	as	as	IN
cord-271701-tx0lqgff	7	17	a	a	DT
cord-271701-tx0lqgff	7	18	primase	primase	NN
cord-271701-tx0lqgff	7	19	.	.	.
cord-271701-tx0lqgff	8	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	8	2	,	,	,
cord-271701-tx0lqgff	8	3	this	this	DT
cord-271701-tx0lqgff	8	4	protein	protein	NN
cord-271701-tx0lqgff	8	5	has	have	VBZ
cord-271701-tx0lqgff	8	6	only	only	RB
cord-271701-tx0lqgff	8	7	been	be	VBN
cord-271701-tx0lqgff	8	8	crystallized	crystallize	VBN
cord-271701-tx0lqgff	8	9	together	together	RB
cord-271701-tx0lqgff	8	10	with	with	IN
cord-271701-tx0lqgff	8	11	the	the	DT
cord-271701-tx0lqgff	8	12	10-kDa	10-kda	CD
cord-271701-tx0lqgff	8	13	nsp7	nsp7	NN
cord-271701-tx0lqgff	8	14	,	,	,
cord-271701-tx0lqgff	8	15	forming	form	VBG
cord-271701-tx0lqgff	8	16	a	a	DT
cord-271701-tx0lqgff	8	17	hexadecameric	hexadecameric	NN
cord-271701-tx0lqgff	8	18	,	,	,
cord-271701-tx0lqgff	8	19	dsRNA	dsrna	RB
cord-271701-tx0lqgff	8	20	-	-	HYPH
cord-271701-tx0lqgff	8	21	encircling	encircle	VBG
cord-271701-tx0lqgff	8	22	ring	ring	NN
cord-271701-tx0lqgff	8	23	structure	structure	NN
cord-271701-tx0lqgff	8	24	[	[	-LRB-
cord-271701-tx0lqgff	8	25	i.e.	i.e.	FW
cord-271701-tx0lqgff	8	26	nsp(7	nsp(7	NN
cord-271701-tx0lqgff	8	27	+	+	SYM
cord-271701-tx0lqgff	8	28	8	8	CD
cord-271701-tx0lqgff	8	29	)	)	-RRB-
cord-271701-tx0lqgff	8	30	,	,	,
cord-271701-tx0lqgff	8	31	consisting	consist	VBG
cord-271701-tx0lqgff	8	32	of	of	IN
cord-271701-tx0lqgff	8	33	8	8	CD
cord-271701-tx0lqgff	8	34	copies	copy	NNS
cord-271701-tx0lqgff	8	35	of	of	IN
cord-271701-tx0lqgff	8	36	both	both	DT
cord-271701-tx0lqgff	8	37	nsps	nsp	NNS
cord-271701-tx0lqgff	8	38	]	]	-RRB-
cord-271701-tx0lqgff	8	39	.	.	.
cord-271701-tx0lqgff	9	1	To	to	TO
cord-271701-tx0lqgff	9	2	better	well	RBR
cord-271701-tx0lqgff	9	3	understand	understand	VB
cord-271701-tx0lqgff	9	4	the	the	DT
cord-271701-tx0lqgff	9	5	implications	implication	NNS
cord-271701-tx0lqgff	9	6	of	of	IN
cord-271701-tx0lqgff	9	7	these	these	DT
cord-271701-tx0lqgff	9	8	structural	structural	JJ
cord-271701-tx0lqgff	9	9	characteristics	characteristic	NNS
cord-271701-tx0lqgff	9	10	for	for	IN
cord-271701-tx0lqgff	9	11	nsp8-driven	nsp8-driven	NNP
cord-271701-tx0lqgff	9	12	RNA	RNA	NNP
cord-271701-tx0lqgff	9	13	synthesis	synthesis	NN
cord-271701-tx0lqgff	9	14	,	,	,
cord-271701-tx0lqgff	9	15	we	-PRON-	PRP
cord-271701-tx0lqgff	9	16	studied	study	VBD
cord-271701-tx0lqgff	9	17	the	the	DT
cord-271701-tx0lqgff	9	18	prerequisites	prerequisite	NNS
cord-271701-tx0lqgff	9	19	for	for	IN
cord-271701-tx0lqgff	9	20	the	the	DT
cord-271701-tx0lqgff	9	21	formation	formation	NN
cord-271701-tx0lqgff	9	22	of	of	IN
cord-271701-tx0lqgff	9	23	the	the	DT
cord-271701-tx0lqgff	9	24	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	9	25	+	+	SYM
cord-271701-tx0lqgff	9	26	8	8	CD
cord-271701-tx0lqgff	9	27	)	)	-RRB-
cord-271701-tx0lqgff	9	28	complex	complex	NN
cord-271701-tx0lqgff	9	29	and	and	CC
cord-271701-tx0lqgff	9	30	its	-PRON-	PRP$
cord-271701-tx0lqgff	9	31	polymerase	polymerase	NN
cord-271701-tx0lqgff	9	32	activity	activity	NN
cord-271701-tx0lqgff	9	33	.	.	.
cord-271701-tx0lqgff	10	1	We	-PRON-	PRP
cord-271701-tx0lqgff	10	2	found	find	VBD
cord-271701-tx0lqgff	10	3	that	that	IN
cord-271701-tx0lqgff	10	4	in	in	IN
cord-271701-tx0lqgff	10	5	particular	particular	JJ
cord-271701-tx0lqgff	10	6	the	the	DT
cord-271701-tx0lqgff	10	7	exposure	exposure	NN
cord-271701-tx0lqgff	10	8	of	of	IN
cord-271701-tx0lqgff	10	9	nsp8	nsp8	NN
cord-271701-tx0lqgff	10	10	's	's	POS
cord-271701-tx0lqgff	10	11	natural	natural	JJ
cord-271701-tx0lqgff	10	12	N	n	CD
cord-271701-tx0lqgff	10	13	-	-	HYPH
cord-271701-tx0lqgff	10	14	terminal	terminal	NN
cord-271701-tx0lqgff	10	15	residue	residue	NN
cord-271701-tx0lqgff	10	16	was	be	VBD
cord-271701-tx0lqgff	10	17	paramount	paramount	JJ
cord-271701-tx0lqgff	10	18	for	for	IN
cord-271701-tx0lqgff	10	19	both	both	CC
cord-271701-tx0lqgff	10	20	the	the	DT
cord-271701-tx0lqgff	10	21	protein	protein	NN
cord-271701-tx0lqgff	10	22	's	's	POS
cord-271701-tx0lqgff	10	23	ability	ability	NN
cord-271701-tx0lqgff	10	24	to	to	TO
cord-271701-tx0lqgff	10	25	associate	associate	VB
cord-271701-tx0lqgff	10	26	with	with	IN
cord-271701-tx0lqgff	10	27	nsp7	nsp7	NNP
cord-271701-tx0lqgff	10	28	and	and	CC
cord-271701-tx0lqgff	10	29	for	for	IN
cord-271701-tx0lqgff	10	30	boosting	boost	VBG
cord-271701-tx0lqgff	10	31	its	-PRON-	PRP$
cord-271701-tx0lqgff	10	32	RdRp	RdRp	NNP
cord-271701-tx0lqgff	10	33	activity	activity	NN
cord-271701-tx0lqgff	10	34	.	.	.
cord-271701-tx0lqgff	11	1	Moreover	moreover	RB
cord-271701-tx0lqgff	11	2	,	,	,
cord-271701-tx0lqgff	11	3	this	this	DT
cord-271701-tx0lqgff	11	4	‘	'	``
cord-271701-tx0lqgff	11	5	improved	improved	JJ
cord-271701-tx0lqgff	11	6	’	'	''
cord-271701-tx0lqgff	11	7	recombinant	recombinant	JJ
cord-271701-tx0lqgff	11	8	nsp8	nsp8	NN
cord-271701-tx0lqgff	11	9	was	be	VBD
cord-271701-tx0lqgff	11	10	capable	capable	JJ
cord-271701-tx0lqgff	11	11	of	of	IN
cord-271701-tx0lqgff	11	12	extending	extend	VBG
cord-271701-tx0lqgff	11	13	primed	prime	VBN
cord-271701-tx0lqgff	11	14	RNA	RNA	NNP
cord-271701-tx0lqgff	11	15	templates	template	NNS
cord-271701-tx0lqgff	11	16	,	,	,
cord-271701-tx0lqgff	11	17	a	a	DT
cord-271701-tx0lqgff	11	18	property	property	NN
cord-271701-tx0lqgff	11	19	that	that	WDT
cord-271701-tx0lqgff	11	20	had	have	VBD
cord-271701-tx0lqgff	11	21	gone	go	VBN
cord-271701-tx0lqgff	11	22	unnoticed	unnoticed	JJ
cord-271701-tx0lqgff	11	23	thus	thus	RB
cord-271701-tx0lqgff	11	24	far	far	RB
cord-271701-tx0lqgff	11	25	.	.	.
cord-271701-tx0lqgff	12	1	The	the	DT
cord-271701-tx0lqgff	12	2	latter	latter	JJ
cord-271701-tx0lqgff	12	3	activity	activity	NN
cord-271701-tx0lqgff	12	4	is	be	VBZ
cord-271701-tx0lqgff	12	5	,	,	,
cord-271701-tx0lqgff	12	6	however	however	RB
cord-271701-tx0lqgff	12	7	,	,	,
cord-271701-tx0lqgff	12	8	∼20-fold	∼20-fold	DT
cord-271701-tx0lqgff	12	9	weaker	weak	JJR
cord-271701-tx0lqgff	12	10	than	than	IN
cord-271701-tx0lqgff	12	11	that	that	DT
cord-271701-tx0lqgff	12	12	of	of	IN
cord-271701-tx0lqgff	12	13	the	the	DT
cord-271701-tx0lqgff	12	14	primer	primer	NN
cord-271701-tx0lqgff	12	15	-	-	HYPH
cord-271701-tx0lqgff	12	16	dependent	dependent	JJ
cord-271701-tx0lqgff	12	17	nsp12-RdRp	nsp12-RdRp	NNS
cord-271701-tx0lqgff	12	18	at	at	IN
cord-271701-tx0lqgff	12	19	equal	equal	JJ
cord-271701-tx0lqgff	12	20	monomer	monomer	NN
cord-271701-tx0lqgff	12	21	concentrations	concentration	NNS
cord-271701-tx0lqgff	12	22	.	.	.
cord-271701-tx0lqgff	13	1	Finally	finally	RB
cord-271701-tx0lqgff	13	2	,	,	,
cord-271701-tx0lqgff	13	3	site	site	NN
cord-271701-tx0lqgff	13	4	-	-	HYPH
cord-271701-tx0lqgff	13	5	directed	direct	VBN
cord-271701-tx0lqgff	13	6	mutagenesis	mutagenesis	NN
cord-271701-tx0lqgff	13	7	of	of	IN
cord-271701-tx0lqgff	13	8	conserved	conserved	JJ
cord-271701-tx0lqgff	13	9	D	D	NNP
cord-271701-tx0lqgff	13	10	/	/	SYM
cord-271701-tx0lqgff	13	11	ExD	exd	NN
cord-271701-tx0lqgff	13	12	/	/	SYM
cord-271701-tx0lqgff	13	13	E	e	NN
cord-271701-tx0lqgff	13	14	motifs	motif	NNS
cord-271701-tx0lqgff	13	15	was	be	VBD
cord-271701-tx0lqgff	13	16	employed	employ	VBN
cord-271701-tx0lqgff	13	17	to	to	TO
cord-271701-tx0lqgff	13	18	identify	identify	VB
cord-271701-tx0lqgff	13	19	residues	residue	NNS
cord-271701-tx0lqgff	13	20	crucial	crucial	JJ
cord-271701-tx0lqgff	13	21	for	for	IN
cord-271701-tx0lqgff	13	22	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	13	23	+	+	SYM
cord-271701-tx0lqgff	13	24	8	8	CD
cord-271701-tx0lqgff	13	25	)	)	-RRB-
cord-271701-tx0lqgff	13	26	RdRp	rdrp	NN
cord-271701-tx0lqgff	13	27	activity	activity	NN
cord-271701-tx0lqgff	13	28	.	.	.
cord-271701-tx0lqgff	14	1	In	in	IN
cord-271701-tx0lqgff	14	2	the	the	DT
cord-271701-tx0lqgff	14	3	replicative	replicative	JJ
cord-271701-tx0lqgff	14	4	cycle	cycle	NN
cord-271701-tx0lqgff	14	5	of	of	IN
cord-271701-tx0lqgff	14	6	RNA	RNA	NNP
cord-271701-tx0lqgff	14	7	viruses	virus	NNS
cord-271701-tx0lqgff	14	8	,	,	,
cord-271701-tx0lqgff	14	9	the	the	DT
cord-271701-tx0lqgff	14	10	crucially	crucially	RB
cord-271701-tx0lqgff	14	11	important	important	JJ
cord-271701-tx0lqgff	14	12	process	process	NN
cord-271701-tx0lqgff	14	13	of	of	IN
cord-271701-tx0lqgff	14	14	RNA	RNA	NNP
cord-271701-tx0lqgff	14	15	-	-	HYPH
cord-271701-tx0lqgff	14	16	templated	templated	JJ
cord-271701-tx0lqgff	14	17	RNA	RNA	NNP
cord-271701-tx0lqgff	14	18	synthesis	synthesis	NN
cord-271701-tx0lqgff	14	19	is	be	VBZ
cord-271701-tx0lqgff	14	20	generally	generally	RB
cord-271701-tx0lqgff	14	21	performed	perform	VBN
cord-271701-tx0lqgff	14	22	by	by	IN
cord-271701-tx0lqgff	14	23	an	an	DT
cord-271701-tx0lqgff	14	24	RNA	RNA	NNP
cord-271701-tx0lqgff	14	25	-	-	HYPH
cord-271701-tx0lqgff	14	26	synthesizing	synthesize	VBG
cord-271701-tx0lqgff	14	27	complex	complex	NN
cord-271701-tx0lqgff	14	28	of	of	IN
cord-271701-tx0lqgff	14	29	viral	viral	JJ
cord-271701-tx0lqgff	14	30	enzymes	enzyme	NNS
cord-271701-tx0lqgff	14	31	(	(	-LRB-
cord-271701-tx0lqgff	14	32	1	1	CD
cord-271701-tx0lqgff	14	33	,	,	,
cord-271701-tx0lqgff	14	34	2	2	CD
cord-271701-tx0lqgff	14	35	)	)	-RRB-
cord-271701-tx0lqgff	14	36	.	.	.
cord-271701-tx0lqgff	15	1	Commonly	commonly	RB
cord-271701-tx0lqgff	15	2	,	,	,
cord-271701-tx0lqgff	15	3	its	-PRON-	PRP$
cord-271701-tx0lqgff	15	4	core	core	NN
cord-271701-tx0lqgff	15	5	subunit	subunit	NN
cord-271701-tx0lqgff	15	6	is	be	VBZ
cord-271701-tx0lqgff	15	7	a	a	DT
cord-271701-tx0lqgff	15	8	single	single	JJ
cord-271701-tx0lqgff	15	9	RNA	RNA	NNP
cord-271701-tx0lqgff	15	10	-	-	HYPH
cord-271701-tx0lqgff	15	11	dependent	dependent	JJ
cord-271701-tx0lqgff	15	12	RNA	RNA	NNP
cord-271701-tx0lqgff	15	13	polymerase	polymerase	NN
cord-271701-tx0lqgff	15	14	(	(	-LRB-
cord-271701-tx0lqgff	15	15	RdRp	RdRp	NNP
cord-271701-tx0lqgff	15	16	)	)	-RRB-
cord-271701-tx0lqgff	15	17	that	that	WDT
cord-271701-tx0lqgff	15	18	drives	drive	VBZ
cord-271701-tx0lqgff	15	19	the	the	DT
cord-271701-tx0lqgff	15	20	production	production	NN
cord-271701-tx0lqgff	15	21	of	of	IN
cord-271701-tx0lqgff	15	22	template	template	NN
cord-271701-tx0lqgff	15	23	strands	strand	NNS
cord-271701-tx0lqgff	15	24	for	for	IN
cord-271701-tx0lqgff	15	25	replication	replication	NN
cord-271701-tx0lqgff	15	26	,	,	,
cord-271701-tx0lqgff	15	27	new	new	JJ
cord-271701-tx0lqgff	15	28	genome	genome	NN
cord-271701-tx0lqgff	15	29	molecules	molecule	NNS
cord-271701-tx0lqgff	15	30	,	,	,
cord-271701-tx0lqgff	15	31	and	and	CC
cord-271701-tx0lqgff	15	32	-	-	:
cord-271701-tx0lqgff	15	33	in	in	IN
cord-271701-tx0lqgff	15	34	many	many	JJ
cord-271701-tx0lqgff	15	35	RNA	RNA	NNP
cord-271701-tx0lqgff	15	36	virus	virus	NN
cord-271701-tx0lqgff	15	37	groupsalso	groupsalso	NNP
cord-271701-tx0lqgff	15	38	subgenomic	subgenomic	NNP
cord-271701-tx0lqgff	15	39	(	(	-LRB-
cord-271701-tx0lqgff	15	40	sg	sg	NNP
cord-271701-tx0lqgff	15	41	)	)	-RRB-
cord-271701-tx0lqgff	15	42	mRNAs	mrnas	CD
cord-271701-tx0lqgff	15	43	.	.	.
cord-271701-tx0lqgff	16	1	This	this	DT
cord-271701-tx0lqgff	16	2	canonical	canonical	JJ
cord-271701-tx0lqgff	16	3	RdRp	rdrp	NN
cord-271701-tx0lqgff	16	4	is	be	VBZ
cord-271701-tx0lqgff	16	5	structurally	structurally	RB
cord-271701-tx0lqgff	16	6	conserved	conserve	VBN
cord-271701-tx0lqgff	16	7	among	among	IN
cord-271701-tx0lqgff	16	8	RNA	RNA	NNP
cord-271701-tx0lqgff	16	9	viruses	virus	NNS
cord-271701-tx0lqgff	16	10	and	and	CC
cord-271701-tx0lqgff	16	11	widely	widely	RB
cord-271701-tx0lqgff	16	12	accepted	accept	VBN
cord-271701-tx0lqgff	16	13	to	to	TO
cord-271701-tx0lqgff	16	14	drive	drive	VB
cord-271701-tx0lqgff	16	15	catalysis	catalysis	NN
cord-271701-tx0lqgff	16	16	of	of	IN
cord-271701-tx0lqgff	16	17	phosphodiester	phosphodiester	NN
cord-271701-tx0lqgff	16	18	bond	bond	NN
cord-271701-tx0lqgff	16	19	formation	formation	NN
cord-271701-tx0lqgff	16	20	via	via	IN
cord-271701-tx0lqgff	16	21	a	a	DT
cord-271701-tx0lqgff	16	22	well	well	RB
cord-271701-tx0lqgff	16	23	-	-	HYPH
cord-271701-tx0lqgff	16	24	established	establish	VBN
cord-271701-tx0lqgff	16	25	reaction	reaction	NN
cord-271701-tx0lqgff	16	26	mechanism	mechanism	NN
cord-271701-tx0lqgff	16	27	involving	involve	VBG
cord-271701-tx0lqgff	16	28	two	two	CD
cord-271701-tx0lqgff	16	29	metal	metal	NN
cord-271701-tx0lqgff	16	30	ions	ion	NNS
cord-271701-tx0lqgff	16	31	that	that	WDT
cord-271701-tx0lqgff	16	32	are	be	VBP
cord-271701-tx0lqgff	16	33	coordinated	coordinate	VBN
cord-271701-tx0lqgff	16	34	by	by	IN
cord-271701-tx0lqgff	16	35	aspartate	aspartate	NN
cord-271701-tx0lqgff	16	36	residues	residue	NNS
cord-271701-tx0lqgff	16	37	in	in	IN
cord-271701-tx0lqgff	16	38	its	-PRON-	PRP$
cord-271701-tx0lqgff	16	39	motifs	motif	NNS
cord-271701-tx0lqgff	16	40	A	a	NN
cord-271701-tx0lqgff	16	41	and	and	CC
cord-271701-tx0lqgff	16	42	C	C	NNP
cord-271701-tx0lqgff	16	43	(	(	-LRB-
cord-271701-tx0lqgff	16	44	3	3	CD
cord-271701-tx0lqgff	16	45	)	)	-RRB-
cord-271701-tx0lqgff	16	46	(	(	-LRB-
cord-271701-tx0lqgff	16	47	4	4	LS
cord-271701-tx0lqgff	16	48	)	)	-RRB-
cord-271701-tx0lqgff	16	49	(	(	-LRB-
cord-271701-tx0lqgff	16	50	5	5	CD
cord-271701-tx0lqgff	16	51	)	)	-RRB-
cord-271701-tx0lqgff	16	52	.	.	.
cord-271701-tx0lqgff	17	1	Uniquely	uniquely	RB
cord-271701-tx0lqgff	17	2	among	among	IN
cord-271701-tx0lqgff	17	3	RNA	RNA	NNP
cord-271701-tx0lqgff	17	4	viruses	virus	NNS
cord-271701-tx0lqgff	17	5	,	,	,
cord-271701-tx0lqgff	17	6	however	however	RB
cord-271701-tx0lqgff	17	7	,	,	,
cord-271701-tx0lqgff	17	8	current	current	JJ
cord-271701-tx0lqgff	17	9	evidence	evidence	NN
cord-271701-tx0lqgff	17	10	suggests	suggest	VBZ
cord-271701-tx0lqgff	17	11	that	that	IN
cord-271701-tx0lqgff	17	12	at	at	RB
cord-271701-tx0lqgff	17	13	least	least	RBS
cord-271701-tx0lqgff	17	14	two	two	CD
cord-271701-tx0lqgff	17	15	RdRp	RdRp	NNP
cord-271701-tx0lqgff	17	16	activities	activity	NNS
cord-271701-tx0lqgff	17	17	are	be	VBP
cord-271701-tx0lqgff	17	18	encoded	encode	VBN
cord-271701-tx0lqgff	17	19	by	by	IN
cord-271701-tx0lqgff	17	20	the	the	DT
cord-271701-tx0lqgff	17	21	genomes	genome	NNS
cord-271701-tx0lqgff	17	22	of	of	IN
cord-271701-tx0lqgff	17	23	members	member	NNS
cord-271701-tx0lqgff	17	24	of	of	IN
cord-271701-tx0lqgff	17	25	the	the	DT
cord-271701-tx0lqgff	17	26	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	17	27	(	(	-LRB-
cord-271701-tx0lqgff	17	28	CoV	CoV	NNP
cord-271701-tx0lqgff	17	29	)	)	-RRB-
cord-271701-tx0lqgff	17	30	family	family	NN
cord-271701-tx0lqgff	17	31	,	,	,
cord-271701-tx0lqgff	17	32	the	the	DT
cord-271701-tx0lqgff	17	33	+	+	SYM
cord-271701-tx0lqgff	17	34	RNA	RNA	NNP
cord-271701-tx0lqgff	17	35	virus	virus	NN
cord-271701-tx0lqgff	17	36	group	group	NN
cord-271701-tx0lqgff	17	37	that	that	WDT
cord-271701-tx0lqgff	17	38	infects	infect	VBZ
cord-271701-tx0lqgff	17	39	a	a	DT
cord-271701-tx0lqgff	17	40	wide	wide	JJ
cord-271701-tx0lqgff	17	41	range	range	NN
cord-271701-tx0lqgff	17	42	of	of	IN
cord-271701-tx0lqgff	17	43	vertebrates	vertebrate	NNS
cord-271701-tx0lqgff	17	44	and	and	CC
cord-271701-tx0lqgff	17	45	is	be	VBZ
cord-271701-tx0lqgff	17	46	renowned	renowned	JJ
cord-271701-tx0lqgff	17	47	for	for	IN
cord-271701-tx0lqgff	17	48	its	-PRON-	PRP$
cord-271701-tx0lqgff	17	49	exceptionally	exceptionally	RB
cord-271701-tx0lqgff	17	50	large	large	JJ
cord-271701-tx0lqgff	17	51	polycistronic	polycistronic	JJ
cord-271701-tx0lqgff	17	52	genome	genome	NN
cord-271701-tx0lqgff	17	53	of	of	IN
cord-271701-tx0lqgff	17	54	$	$	$
cord-271701-tx0lqgff	17	55	30	30	CD
cord-271701-tx0lqgff	17	56	kilobases	kilobase	NNS
cord-271701-tx0lqgff	17	57	(	(	-LRB-
cord-271701-tx0lqgff	17	58	6	6	CD
cord-271701-tx0lqgff	17	59	)	)	-RRB-
cord-271701-tx0lqgff	17	60	.	.	.
cord-271701-tx0lqgff	18	1	Both	both	DT
cord-271701-tx0lqgff	18	2	CoV	CoV	NNP
cord-271701-tx0lqgff	18	3	RdRps	RdRps	NNP
cord-271701-tx0lqgff	18	4	belong	belong	VBP
cord-271701-tx0lqgff	18	5	to	to	IN
cord-271701-tx0lqgff	18	6	the	the	DT
cord-271701-tx0lqgff	18	7	set	set	NN
cord-271701-tx0lqgff	18	8	of	of	IN
cord-271701-tx0lqgff	18	9	16	16	CD
cord-271701-tx0lqgff	18	10	non	non	JJ
cord-271701-tx0lqgff	18	11	-	-	JJ
cord-271701-tx0lqgff	18	12	structural	structural	JJ
cord-271701-tx0lqgff	18	13	proteins	protein	NNS
cord-271701-tx0lqgff	18	14	(	(	-LRB-
cord-271701-tx0lqgff	18	15	nsps	nsps	NNP
cord-271701-tx0lqgff	18	16	)	)	-RRB-
cord-271701-tx0lqgff	18	17	that	that	WDT
cord-271701-tx0lqgff	18	18	are	be	VBP
cord-271701-tx0lqgff	18	19	produced	produce	VBN
cord-271701-tx0lqgff	18	20	through	through	IN
cord-271701-tx0lqgff	18	21	proteolytic	proteolytic	JJ
cord-271701-tx0lqgff	18	22	processing	processing	NN
cord-271701-tx0lqgff	18	23	of	of	IN
cord-271701-tx0lqgff	18	24	the	the	DT
cord-271701-tx0lqgff	18	25	pp1a	pp1a	NNP
cord-271701-tx0lqgff	18	26	and	and	CC
cord-271701-tx0lqgff	18	27	pp1ab	pp1ab	NNP
cord-271701-tx0lqgff	18	28	replicase	replicase	NN
cord-271701-tx0lqgff	18	29	precursor	precursor	NN
cord-271701-tx0lqgff	18	30	polyproteins	polyprotein	NNS
cord-271701-tx0lqgff	18	31	,	,	,
cord-271701-tx0lqgff	18	32	which	which	WDT
cord-271701-tx0lqgff	18	33	both	both	DT
cord-271701-tx0lqgff	18	34	derive	derive	VBP
cord-271701-tx0lqgff	18	35	from	from	IN
cord-271701-tx0lqgff	18	36	translation	translation	NN
cord-271701-tx0lqgff	18	37	of	of	IN
cord-271701-tx0lqgff	18	38	the	the	DT
cord-271701-tx0lqgff	18	39	genomic	genomic	JJ
cord-271701-tx0lqgff	18	40	RNA	RNA	NNP
cord-271701-tx0lqgff	18	41	(	(	-LRB-
cord-271701-tx0lqgff	18	42	7	7	CD
cord-271701-tx0lqgff	18	43	,	,	,
cord-271701-tx0lqgff	18	44	8)	8)	NN
cord-271701-tx0lqgff	18	45	.	.	.
cord-271701-tx0lqgff	19	1	For	for	IN
cord-271701-tx0lqgff	19	2	the	the	DT
cord-271701-tx0lqgff	19	3	Severe	severe	JJ
cord-271701-tx0lqgff	19	4	Acute	acute	JJ
cord-271701-tx0lqgff	19	5	Respiratory	Respiratory	NNP
cord-271701-tx0lqgff	19	6	Syndrome	syndrome	NN
cord-271701-tx0lqgff	19	7	-	-	HYPH
cord-271701-tx0lqgff	19	8	associated	associate	VBN
cord-271701-tx0lqgff	19	9	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	19	10	(	(	-LRB-
cord-271701-tx0lqgff	19	11	SARS	SARS	NNP
cord-271701-tx0lqgff	19	12	-	-	HYPH
cord-271701-tx0lqgff	19	13	CoV	CoV	NNP
cord-271701-tx0lqgff	19	14	)	)	-RRB-
cord-271701-tx0lqgff	19	15	,	,	,
cord-271701-tx0lqgff	19	16	which	which	WDT
cord-271701-tx0lqgff	19	17	emerged	emerge	VBD
cord-271701-tx0lqgff	19	18	in	in	IN
cord-271701-tx0lqgff	19	19	2003	2003	CD
cord-271701-tx0lqgff	19	20	and	and	CC
cord-271701-tx0lqgff	19	21	caused	cause	VBD
cord-271701-tx0lqgff	19	22	worldwide	worldwide	JJ
cord-271701-tx0lqgff	19	23	concern	concern	NN
cord-271701-tx0lqgff	19	24	due	due	IN
cord-271701-tx0lqgff	19	25	to	to	IN
cord-271701-tx0lqgff	19	26	the	the	DT
cord-271701-tx0lqgff	19	27	$	$	$
cord-271701-tx0lqgff	19	28	10	10	CD
cord-271701-tx0lqgff	19	29	%	%	NN
cord-271701-tx0lqgff	19	30	mortality	mortality	NN
cord-271701-tx0lqgff	19	31	rate	rate	NN
cord-271701-tx0lqgff	19	32	associated	associate	VBN
cord-271701-tx0lqgff	19	33	with	with	IN
cord-271701-tx0lqgff	19	34	infection	infection	NN
cord-271701-tx0lqgff	19	35	of	of	IN
cord-271701-tx0lqgff	19	36	humans	human	NNS
cord-271701-tx0lqgff	19	37	(	(	-LRB-
cord-271701-tx0lqgff	19	38	6	6	CD
cord-271701-tx0lqgff	19	39	,	,	,
cord-271701-tx0lqgff	19	40	9	9	CD
cord-271701-tx0lqgff	19	41	)	)	-RRB-
cord-271701-tx0lqgff	19	42	,	,	,
cord-271701-tx0lqgff	19	43	the	the	DT
cord-271701-tx0lqgff	19	44	two	two	CD
cord-271701-tx0lqgff	19	45	replicase	replicase	NN
cord-271701-tx0lqgff	19	46	subunits	subunit	NNS
cord-271701-tx0lqgff	19	47	with	with	IN
cord-271701-tx0lqgff	19	48	RdRp	RdRp	NNP
cord-271701-tx0lqgff	19	49	activity	activity	NN
cord-271701-tx0lqgff	19	50	have	have	VBP
cord-271701-tx0lqgff	19	51	been	be	VBN
cord-271701-tx0lqgff	19	52	studied	study	VBN
cord-271701-tx0lqgff	19	53	in	in	IN
cord-271701-tx0lqgff	19	54	some	some	DT
cord-271701-tx0lqgff	19	55	detail	detail	NN
cord-271701-tx0lqgff	19	56	.	.	.
cord-271701-tx0lqgff	20	1	The	the	DT
cord-271701-tx0lqgff	20	2	first	first	JJ
cord-271701-tx0lqgff	20	3	is	be	VBZ
cord-271701-tx0lqgff	20	4	the	the	DT
cord-271701-tx0lqgff	20	5	106-kDa	106-kda	CD
cord-271701-tx0lqgff	20	6	nsp12	nsp12	NNS
cord-271701-tx0lqgff	20	7	,	,	,
cord-271701-tx0lqgff	20	8	which	which	WDT
cord-271701-tx0lqgff	20	9	contains	contain	VBZ
cord-271701-tx0lqgff	20	10	the	the	DT
cord-271701-tx0lqgff	20	11	canonical	canonical	JJ
cord-271701-tx0lqgff	20	12	viral	viral	JJ
cord-271701-tx0lqgff	20	13	RdRp	RdRp	NNP
cord-271701-tx0lqgff	20	14	motifs	motif	NNS
cord-271701-tx0lqgff	20	15	in	in	IN
cord-271701-tx0lqgff	20	16	its	-PRON-	PRP$
cord-271701-tx0lqgff	20	17	C	c	NN
cord-271701-tx0lqgff	20	18	-	-	HYPH
cord-271701-tx0lqgff	20	19	terminal	terminal	NN
cord-271701-tx0lqgff	20	20	part	part	NN
cord-271701-tx0lqgff	20	21	and	and	CC
cord-271701-tx0lqgff	20	22	employs	employ	VBZ
cord-271701-tx0lqgff	20	23	a	a	DT
cord-271701-tx0lqgff	20	24	primerdependent	primerdependent	NN
cord-271701-tx0lqgff	20	25	initiation	initiation	NN
cord-271701-tx0lqgff	20	26	mechanism	mechanism	NN
cord-271701-tx0lqgff	20	27	(	(	-LRB-
cord-271701-tx0lqgff	20	28	10	10	CD
cord-271701-tx0lqgff	20	29	,	,	,
cord-271701-tx0lqgff	20	30	11	11	CD
cord-271701-tx0lqgff	20	31	)	)	-RRB-
cord-271701-tx0lqgff	20	32	.	.	.
cord-271701-tx0lqgff	21	1	The	the	DT
cord-271701-tx0lqgff	21	2	second	second	JJ
cord-271701-tx0lqgff	21	3	polymerase	polymerase	NN
cord-271701-tx0lqgff	21	4	,	,	,
cord-271701-tx0lqgff	21	5	the	the	DT
cord-271701-tx0lqgff	21	6	22-kDa	22-kda	CD
cord-271701-tx0lqgff	21	7	nsp8	nsp8	NN
cord-271701-tx0lqgff	21	8	,	,	,
cord-271701-tx0lqgff	21	9	is	be	VBZ
cord-271701-tx0lqgff	21	10	unique	unique	JJ
cord-271701-tx0lqgff	21	11	for	for	IN
cord-271701-tx0lqgff	21	12	CoVs	CoVs	NNP
cord-271701-tx0lqgff	21	13	and	and	CC
cord-271701-tx0lqgff	21	14	was	be	VBD
cord-271701-tx0lqgff	21	15	reported	report	VBN
cord-271701-tx0lqgff	21	16	to	to	TO
cord-271701-tx0lqgff	21	17	be	be	VB
cord-271701-tx0lqgff	21	18	only	only	RB
cord-271701-tx0lqgff	21	19	capable	capable	JJ
cord-271701-tx0lqgff	21	20	of	of	IN
cord-271701-tx0lqgff	21	21	de	de	NNP
cord-271701-tx0lqgff	21	22	novo	novo	NNP
cord-271701-tx0lqgff	21	23	RNA	RNA	NNP
cord-271701-tx0lqgff	21	24	synthesis	synthesis	NN
cord-271701-tx0lqgff	21	25	with	with	IN
cord-271701-tx0lqgff	21	26	a	a	DT
cord-271701-tx0lqgff	21	27	low	low	JJ
cord-271701-tx0lqgff	21	28	fidelity	fidelity	NN
cord-271701-tx0lqgff	21	29	on	on	IN
cord-271701-tx0lqgff	21	30	ssRNA	ssrna	ADD
cord-271701-tx0lqgff	21	31	templates	template	NNS
cord-271701-tx0lqgff	21	32	(	(	-LRB-
cord-271701-tx0lqgff	21	33	12	12	CD
cord-271701-tx0lqgff	21	34	)	)	-RRB-
cord-271701-tx0lqgff	21	35	.	.	.
cord-271701-tx0lqgff	22	1	Together	together	RB
cord-271701-tx0lqgff	22	2	,	,	,
cord-271701-tx0lqgff	22	3	these	these	DT
cord-271701-tx0lqgff	22	4	observations	observation	NNS
cord-271701-tx0lqgff	22	5	inspired	inspire	VBD
cord-271701-tx0lqgff	22	6	a	a	DT
cord-271701-tx0lqgff	22	7	hypothesis	hypothesis	NN
cord-271701-tx0lqgff	22	8	in	in	IN
cord-271701-tx0lqgff	22	9	which	which	WDT
cord-271701-tx0lqgff	22	10	nsp8	nsp8	NN
cord-271701-tx0lqgff	22	11	would	would	MD
cord-271701-tx0lqgff	22	12	serve	serve	VB
cord-271701-tx0lqgff	22	13	as	as	IN
cord-271701-tx0lqgff	22	14	an	an	DT
cord-271701-tx0lqgff	22	15	RNA	RNA	NNP
cord-271701-tx0lqgff	22	16	primase	primase	NN
cord-271701-tx0lqgff	22	17	,	,	,
cord-271701-tx0lqgff	22	18	i.e.	i.e.	FW
cord-271701-tx0lqgff	22	19	would	would	MD
cord-271701-tx0lqgff	22	20	synthesise	synthesise	VB
cord-271701-tx0lqgff	22	21	short	short	JJ
cord-271701-tx0lqgff	22	22	oligonucleotide	oligonucleotide	JJ
cord-271701-tx0lqgff	22	23	primers	primer	NNS
cord-271701-tx0lqgff	22	24	for	for	IN
cord-271701-tx0lqgff	22	25	subsequent	subsequent	JJ
cord-271701-tx0lqgff	22	26	extension	extension	NN
cord-271701-tx0lqgff	22	27	by	by	IN
cord-271701-tx0lqgff	22	28	the	the	DT
cord-271701-tx0lqgff	22	29	nsp12	nsp12	NNP
cord-271701-tx0lqgff	22	30	'	'	POS
cord-271701-tx0lqgff	22	31	main	main	JJ
cord-271701-tx0lqgff	22	32	RdRp	RdRp	NNP
cord-271701-tx0lqgff	22	33	'	'	''
cord-271701-tx0lqgff	22	34	(	(	-LRB-
cord-271701-tx0lqgff	22	35	12	12	CD
cord-271701-tx0lqgff	22	36	)	)	-RRB-
cord-271701-tx0lqgff	22	37	.	.	.
cord-271701-tx0lqgff	23	1	In	in	IN
cord-271701-tx0lqgff	23	2	spite	spite	NN
cord-271701-tx0lqgff	23	3	of	of	IN
cord-271701-tx0lqgff	23	4	this	this	DT
cord-271701-tx0lqgff	23	5	attractive	attractive	JJ
cord-271701-tx0lqgff	23	6	model	model	NN
cord-271701-tx0lqgff	23	7	,	,	,
cord-271701-tx0lqgff	23	8	however	however	RB
cord-271701-tx0lqgff	23	9	,	,	,
cord-271701-tx0lqgff	23	10	many	many	JJ
cord-271701-tx0lqgff	23	11	questions	question	NNS
cord-271701-tx0lqgff	23	12	regarding	regard	VBG
cord-271701-tx0lqgff	23	13	CoV	CoV	NNP
cord-271701-tx0lqgff	23	14	RNA	RNA	NNP
cord-271701-tx0lqgff	23	15	synthesis	synthesis	NN
cord-271701-tx0lqgff	23	16	remain	remain	VBP
cord-271701-tx0lqgff	23	17	unanswered	unanswered	JJ
cord-271701-tx0lqgff	23	18	thus	thus	RB
cord-271701-tx0lqgff	23	19	far	far	RB
cord-271701-tx0lqgff	23	20	.	.	.
cord-271701-tx0lqgff	24	1	For	for	IN
cord-271701-tx0lqgff	24	2	instance	instance	NN
cord-271701-tx0lqgff	24	3	,	,	,
cord-271701-tx0lqgff	24	4	it	-PRON-	PRP
cord-271701-tx0lqgff	24	5	is	be	VBZ
cord-271701-tx0lqgff	24	6	unclear	unclear	JJ
cord-271701-tx0lqgff	24	7	whether	whether	IN
cord-271701-tx0lqgff	24	8	the	the	DT
cord-271701-tx0lqgff	24	9	homomeric	homomeric	NNP
cord-271701-tx0lqgff	24	10	form	form	NN
cord-271701-tx0lqgff	24	11	of	of	IN
cord-271701-tx0lqgff	24	12	nsp8	nsp8	NN
cord-271701-tx0lqgff	24	13	,	,	,
cord-271701-tx0lqgff	24	14	for	for	IN
cord-271701-tx0lqgff	24	15	which	which	WDT
cord-271701-tx0lqgff	24	16	in	in	IN
cord-271701-tx0lqgff	24	17	vitro	vitro	FW
cord-271701-tx0lqgff	24	18	RdRp	RdRp	NNP
cord-271701-tx0lqgff	24	19	activity	activity	NN
cord-271701-tx0lqgff	24	20	was	be	VBD
cord-271701-tx0lqgff	24	21	previously	previously	RB
cord-271701-tx0lqgff	24	22	documented	document	VBN
cord-271701-tx0lqgff	24	23	(	(	-LRB-
cord-271701-tx0lqgff	24	24	12	12	CD
cord-271701-tx0lqgff	24	25	)	)	-RRB-
cord-271701-tx0lqgff	24	26	,	,	,
cord-271701-tx0lqgff	24	27	actually	actually	RB
cord-271701-tx0lqgff	24	28	occurs	occur	VBZ
cord-271701-tx0lqgff	24	29	in	in	IN
cord-271701-tx0lqgff	24	30	vivo	vivo	NN
cord-271701-tx0lqgff	24	31	,	,	,
cord-271701-tx0lqgff	24	32	as	as	IN
cord-271701-tx0lqgff	24	33	nsp8	nsp8	NN
cord-271701-tx0lqgff	24	34	was	be	VBD
cord-271701-tx0lqgff	24	35	also	also	RB
cord-271701-tx0lqgff	24	36	shown	show	VBN
cord-271701-tx0lqgff	24	37	to	to	TO
cord-271701-tx0lqgff	24	38	co	co	VB
cord-271701-tx0lqgff	24	39	-	-	VB
cord-271701-tx0lqgff	24	40	crystallize	crystallize	VB
cord-271701-tx0lqgff	24	41	and	and	CC
cord-271701-tx0lqgff	24	42	form	form	VB
cord-271701-tx0lqgff	24	43	a	a	DT
cord-271701-tx0lqgff	24	44	unique	unique	JJ
cord-271701-tx0lqgff	24	45	hexadecameric	hexadecameric	JJ
cord-271701-tx0lqgff	24	46	ring	ring	NN
cord-271701-tx0lqgff	24	47	-	-	HYPH
cord-271701-tx0lqgff	24	48	structure	structure	NN
cord-271701-tx0lqgff	24	49	with	with	IN
cord-271701-tx0lqgff	24	50	the	the	DT
cord-271701-tx0lqgff	24	51	10-kDa	10-kda	CD
cord-271701-tx0lqgff	24	52	nsp7	nsp7	NNP
cord-271701-tx0lqgff	24	53	subunit	subunit	NNP
cord-271701-tx0lqgff	24	54	that	that	WDT
cord-271701-tx0lqgff	24	55	resides	reside	VBZ
cord-271701-tx0lqgff	24	56	immediately	immediately	RB
cord-271701-tx0lqgff	24	57	upstream	upstream	RB
cord-271701-tx0lqgff	24	58	in	in	IN
cord-271701-tx0lqgff	24	59	the	the	DT
cord-271701-tx0lqgff	24	60	replicase	replicase	NN
cord-271701-tx0lqgff	24	61	polyprotein	polyprotein	NNP
cord-271701-tx0lqgff	24	62	precursors	precursor	NNS
cord-271701-tx0lqgff	24	63	(	(	-LRB-
cord-271701-tx0lqgff	24	64	Figure	figure	NN
cord-271701-tx0lqgff	24	65	1	1	CD
cord-271701-tx0lqgff	24	66	)	)	-RRB-
cord-271701-tx0lqgff	24	67	(	(	-LRB-
cord-271701-tx0lqgff	24	68	13	13	CD
cord-271701-tx0lqgff	24	69	)	)	-RRB-
cord-271701-tx0lqgff	24	70	.	.	.
cord-271701-tx0lqgff	25	1	In	in	IN
cord-271701-tx0lqgff	25	2	a	a	DT
cord-271701-tx0lqgff	25	3	similar	similar	JJ
cord-271701-tx0lqgff	25	4	fashion	fashion	NN
cord-271701-tx0lqgff	25	5	,	,	,
cord-271701-tx0lqgff	25	6	it	-PRON-	PRP
cord-271701-tx0lqgff	25	7	is	be	VBZ
cord-271701-tx0lqgff	25	8	presently	presently	RB
cord-271701-tx0lqgff	25	9	unknown	unknown	JJ
cord-271701-tx0lqgff	25	10	whether	whether	IN
cord-271701-tx0lqgff	25	11	the	the	DT
cord-271701-tx0lqgff	25	12	postulated	postulate	VBN
cord-271701-tx0lqgff	25	13	double	double	RB
cord-271701-tx0lqgff	25	14	-	-	HYPH
cord-271701-tx0lqgff	25	15	stranded	strand	VBN
cord-271701-tx0lqgff	25	16	RNA	RNA	NNP
cord-271701-tx0lqgff	25	17	(	(	-LRB-
cord-271701-tx0lqgff	25	18	dsRNA	dsrna	NN
cord-271701-tx0lqgff	25	19	)	)	-RRB-
cord-271701-tx0lqgff	25	20	binding	bind	VBG
cord-271701-tx0lqgff	25	21	channel	channel	NN
cord-271701-tx0lqgff	25	22	of	of	IN
cord-271701-tx0lqgff	25	23	this	this	DT
cord-271701-tx0lqgff	25	24	complex	complex	NN
cord-271701-tx0lqgff	25	25	plays	play	VBZ
cord-271701-tx0lqgff	25	26	a	a	DT
cord-271701-tx0lqgff	25	27	role	role	NN
cord-271701-tx0lqgff	25	28	in	in	IN
cord-271701-tx0lqgff	25	29	the	the	DT
cord-271701-tx0lqgff	25	30	RdRp	RdRp	NNP
cord-271701-tx0lqgff	25	31	activity	activity	NN
cord-271701-tx0lqgff	25	32	of	of	IN
cord-271701-tx0lqgff	25	33	nsp8	nsp8	NN
cord-271701-tx0lqgff	25	34	and	and	CC
cord-271701-tx0lqgff	25	35	whether	whether	IN
cord-271701-tx0lqgff	25	36	this	this	DT
cord-271701-tx0lqgff	25	37	activity	activity	NN
cord-271701-tx0lqgff	25	38	is	be	VBZ
cord-271701-tx0lqgff	25	39	influenced	influence	VBN
cord-271701-tx0lqgff	25	40	by	by	IN
cord-271701-tx0lqgff	25	41	nsp7	nsp7	NNP
cord-271701-tx0lqgff	25	42	,	,	,
cord-271701-tx0lqgff	25	43	particularly	particularly	RB
cord-271701-tx0lqgff	25	44	given	give	VBN
cord-271701-tx0lqgff	25	45	the	the	DT
cord-271701-tx0lqgff	25	46	observed	observed	JJ
cord-271701-tx0lqgff	25	47	low	low	JJ
cord-271701-tx0lqgff	25	48	fidelity	fidelity	NN
cord-271701-tx0lqgff	25	49	and	and	CC
cord-271701-tx0lqgff	25	50	low	low	JJ
cord-271701-tx0lqgff	25	51	processivity	processivity	NN
cord-271701-tx0lqgff	25	52	of	of	IN
cord-271701-tx0lqgff	25	53	nsp8	nsp8	NN
cord-271701-tx0lqgff	25	54	(	(	-LRB-
cord-271701-tx0lqgff	25	55	12	12	CD
cord-271701-tx0lqgff	25	56	)	)	-RRB-
cord-271701-tx0lqgff	25	57	.	.	.
cord-271701-tx0lqgff	26	1	To	to	TO
cord-271701-tx0lqgff	26	2	investigate	investigate	VB
cord-271701-tx0lqgff	26	3	the	the	DT
cord-271701-tx0lqgff	26	4	properties	property	NNS
cord-271701-tx0lqgff	26	5	of	of	IN
cord-271701-tx0lqgff	26	6	the	the	DT
cord-271701-tx0lqgff	26	7	nsp7+nsp8	nsp7+nsp8	NNP
cord-271701-tx0lqgff	26	8	[	[	-LRB-
cord-271701-tx0lqgff	26	9	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	26	10	+	+	SYM
cord-271701-tx0lqgff	26	11	8	8	CD
cord-271701-tx0lqgff	26	12	)	)	-RRB-
cord-271701-tx0lqgff	26	13	]	]	-RRB-
cord-271701-tx0lqgff	27	1	hexadecamer	hexadecamer	NNP
cord-271701-tx0lqgff	27	2	in	in	IN
cord-271701-tx0lqgff	27	3	more	more	JJR
cord-271701-tx0lqgff	27	4	detail	detail	NN
cord-271701-tx0lqgff	27	5	,	,	,
cord-271701-tx0lqgff	27	6	and	and	CC
cord-271701-tx0lqgff	27	7	seek	seek	VB
cord-271701-tx0lqgff	27	8	answers	answer	NNS
cord-271701-tx0lqgff	27	9	to	to	IN
cord-271701-tx0lqgff	27	10	the	the	DT
cord-271701-tx0lqgff	27	11	above	above	JJ
cord-271701-tx0lqgff	27	12	questions	question	NNS
cord-271701-tx0lqgff	27	13	,	,	,
cord-271701-tx0lqgff	27	14	we	-PRON-	PRP
cord-271701-tx0lqgff	27	15	here	here	RB
cord-271701-tx0lqgff	27	16	generated	generate	VBD
cord-271701-tx0lqgff	27	17	and	and	CC
cord-271701-tx0lqgff	27	18	purified	purify	VBN
cord-271701-tx0lqgff	27	19	recombinant	recombinant	JJ
cord-271701-tx0lqgff	27	20	forms	form	NNS
cord-271701-tx0lqgff	27	21	of	of	IN
cord-271701-tx0lqgff	27	22	SARS	SARS	NNP
cord-271701-tx0lqgff	27	23	-	-	HYPH
cord-271701-tx0lqgff	27	24	CoV	CoV	NNP
cord-271701-tx0lqgff	27	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	27	26	and	and	CC
cord-271701-tx0lqgff	27	27	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	27	28	+	+	SYM
cord-271701-tx0lqgff	27	29	8	8	CD
cord-271701-tx0lqgff	27	30	)	)	-RRB-
cord-271701-tx0lqgff	27	31	that	that	WDT
cord-271701-tx0lqgff	27	32	have	have	VBP
cord-271701-tx0lqgff	27	33	natural	natural	JJ
cord-271701-tx0lqgff	27	34	N	n	CD
cord-271701-tx0lqgff	27	35	-	-	HYPH
cord-271701-tx0lqgff	27	36	terminal	terminal	NN
cord-271701-tx0lqgff	27	37	residues	residue	NNS
cord-271701-tx0lqgff	27	38	.	.	.
cord-271701-tx0lqgff	28	1	This	this	DT
cord-271701-tx0lqgff	28	2	technical	technical	JJ
cord-271701-tx0lqgff	28	3	refinement	refinement	NN
cord-271701-tx0lqgff	28	4	was	be	VBD
cord-271701-tx0lqgff	28	5	found	find	VBN
cord-271701-tx0lqgff	28	6	to	to	TO
cord-271701-tx0lqgff	28	7	greatly	greatly	RB
cord-271701-tx0lqgff	28	8	improve	improve	VB
cord-271701-tx0lqgff	28	9	nsp8	nsp8	NN
cord-271701-tx0lqgff	28	10	's	's	POS
cord-271701-tx0lqgff	28	11	ability	ability	NN
cord-271701-tx0lqgff	28	12	to	to	TO
cord-271701-tx0lqgff	28	13	associate	associate	VB
cord-271701-tx0lqgff	28	14	with	with	IN
cord-271701-tx0lqgff	28	15	nsp7	nsp7	NNP
cord-271701-tx0lqgff	28	16	.	.	.
cord-271701-tx0lqgff	29	1	Moreover	moreover	RB
cord-271701-tx0lqgff	29	2	,	,	,
cord-271701-tx0lqgff	29	3	and	and	CC
cord-271701-tx0lqgff	29	4	in	in	IN
cord-271701-tx0lqgff	29	5	contrast	contrast	NN
cord-271701-tx0lqgff	29	6	to	to	IN
cord-271701-tx0lqgff	29	7	previous	previous	JJ
cord-271701-tx0lqgff	29	8	observations	observation	NNS
cord-271701-tx0lqgff	29	9	(	(	-LRB-
cord-271701-tx0lqgff	29	10	12	12	CD
cord-271701-tx0lqgff	29	11	)	)	-RRB-
cord-271701-tx0lqgff	29	12	,	,	,
cord-271701-tx0lqgff	29	13	exposure	exposure	NN
cord-271701-tx0lqgff	29	14	of	of	IN
cord-271701-tx0lqgff	29	15	the	the	DT
cord-271701-tx0lqgff	29	16	natural	natural	JJ
cord-271701-tx0lqgff	29	17	N	n	CD
cord-271701-tx0lqgff	29	18	-	-	HYPH
cord-271701-tx0lqgff	29	19	terminus	terminus	NN
cord-271701-tx0lqgff	29	20	proved	prove	VBD
cord-271701-tx0lqgff	29	21	crucial	crucial	JJ
cord-271701-tx0lqgff	29	22	for	for	IN
cord-271701-tx0lqgff	29	23	the	the	DT
cord-271701-tx0lqgff	29	24	enzymatic	enzymatic	JJ
cord-271701-tx0lqgff	29	25	activity	activity	NN
cord-271701-tx0lqgff	29	26	of	of	IN
cord-271701-tx0lqgff	29	27	the	the	DT
cord-271701-tx0lqgff	29	28	complex	complex	NN
cord-271701-tx0lqgff	29	29	on	on	IN
cord-271701-tx0lqgff	29	30	partially	partially	RB
cord-271701-tx0lqgff	29	31	double	double	RB
cord-271701-tx0lqgff	29	32	-	-	HYPH
cord-271701-tx0lqgff	29	33	stranded	strand	VBN
cord-271701-tx0lqgff	29	34	RNA	RNA	NNP
cord-271701-tx0lqgff	29	35	templates	template	NNS
cord-271701-tx0lqgff	29	36	,	,	,
cord-271701-tx0lqgff	29	37	demonstrating	demonstrate	VBG
cord-271701-tx0lqgff	29	38	that	that	IN
cord-271701-tx0lqgff	29	39	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	29	40	+	+	SYM
cord-271701-tx0lqgff	29	41	8	8	CD
cord-271701-tx0lqgff	29	42	)	)	-RRB-
cord-271701-tx0lqgff	29	43	is	be	VBZ
cord-271701-tx0lqgff	29	44	capable	capable	JJ
cord-271701-tx0lqgff	29	45	of	of	IN
cord-271701-tx0lqgff	29	46	primerdependent	primerdependent	NN
cord-271701-tx0lqgff	29	47	RdRp	RdRp	NNP
cord-271701-tx0lqgff	29	48	activity	activity	NN
cord-271701-tx0lqgff	29	49	as	as	RB
cord-271701-tx0lqgff	29	50	well	well	RB
cord-271701-tx0lqgff	29	51	.	.	.
cord-271701-tx0lqgff	30	1	Site	site	NN
cord-271701-tx0lqgff	30	2	-	-	HYPH
cord-271701-tx0lqgff	30	3	directed	direct	VBN
cord-271701-tx0lqgff	30	4	mutagenesis	mutagenesis	NN
cord-271701-tx0lqgff	30	5	of	of	IN
cord-271701-tx0lqgff	30	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	30	7	in	in	IN
cord-271701-tx0lqgff	30	8	the	the	DT
cord-271701-tx0lqgff	30	9	context	context	NN
cord-271701-tx0lqgff	30	10	of	of	IN
cord-271701-tx0lqgff	30	11	the	the	DT
cord-271701-tx0lqgff	30	12	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	30	13	+	+	SYM
cord-271701-tx0lqgff	30	14	8	8	CD
cord-271701-tx0lqgff	30	15	)	)	-RRB-
cord-271701-tx0lqgff	30	16	complex	complex	NN
cord-271701-tx0lqgff	30	17	identified	identify	VBD
cord-271701-tx0lqgff	30	18	a	a	DT
cord-271701-tx0lqgff	30	19	conserved	conserved	JJ
cord-271701-tx0lqgff	30	20	D	D	NNP
cord-271701-tx0lqgff	30	21	/	/	SYM
cord-271701-tx0lqgff	30	22	ExD	exd	NN
cord-271701-tx0lqgff	30	23	/	/	SYM
cord-271701-tx0lqgff	30	24	E	e	NN
cord-271701-tx0lqgff	30	25	motif	motif	NN
cord-271701-tx0lqgff	30	26	that	that	WDT
cord-271701-tx0lqgff	30	27	is	be	VBZ
cord-271701-tx0lqgff	30	28	important	important	JJ
cord-271701-tx0lqgff	30	29	for	for	IN
cord-271701-tx0lqgff	30	30	catalysis	catalysis	NN
cord-271701-tx0lqgff	30	31	in	in	IN
cord-271701-tx0lqgff	30	32	vitro	vitro	FW
cord-271701-tx0lqgff	30	33	,	,	,
cord-271701-tx0lqgff	30	34	possibly	possibly	RB
cord-271701-tx0lqgff	30	35	providing	provide	VBG
cord-271701-tx0lqgff	30	36	a	a	DT
cord-271701-tx0lqgff	30	37	first	first	JJ
cord-271701-tx0lqgff	30	38	indication	indication	NN
cord-271701-tx0lqgff	30	39	of	of	IN
cord-271701-tx0lqgff	30	40	the	the	DT
cord-271701-tx0lqgff	30	41	location	location	NN
cord-271701-tx0lqgff	30	42	of	of	IN
cord-271701-tx0lqgff	30	43	the	the	DT
cord-271701-tx0lqgff	30	44	presently	presently	RB
cord-271701-tx0lqgff	30	45	unknown	unknown	JJ
cord-271701-tx0lqgff	30	46	nsp8	nsp8	NN
cord-271701-tx0lqgff	30	47	active	active	JJ
cord-271701-tx0lqgff	30	48	site	site	NN
cord-271701-tx0lqgff	30	49	.	.	.
cord-271701-tx0lqgff	31	1	Overall	overall	RB
cord-271701-tx0lqgff	31	2	,	,	,
cord-271701-tx0lqgff	31	3	these	these	DT
cord-271701-tx0lqgff	31	4	results	result	NNS
cord-271701-tx0lqgff	31	5	define	define	VBP
cord-271701-tx0lqgff	31	6	the	the	DT
cord-271701-tx0lqgff	31	7	SARS	SARS	NNP
cord-271701-tx0lqgff	31	8	-	-	HYPH
cord-271701-tx0lqgff	31	9	CoV	CoV	NNP
cord-271701-tx0lqgff	31	10	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	31	11	+	+	SYM
cord-271701-tx0lqgff	31	12	8	8	CD
cord-271701-tx0lqgff	31	13	)	)	-RRB-
cord-271701-tx0lqgff	31	14	complex	complex	JJ
cord-271701-tx0lqgff	31	15	as	as	IN
cord-271701-tx0lqgff	31	16	an	an	DT
cord-271701-tx0lqgff	31	17	intriguing	intriguing	JJ
cord-271701-tx0lqgff	31	18	multimeric	multimeric	JJ
cord-271701-tx0lqgff	31	19	RNA	RNA	NNP
cord-271701-tx0lqgff	31	20	polymerase	polymerase	NN
cord-271701-tx0lqgff	31	21	that	that	WDT
cord-271701-tx0lqgff	31	22	is	be	VBZ
cord-271701-tx0lqgff	31	23	capable	capable	JJ
cord-271701-tx0lqgff	31	24	of	of	IN
cord-271701-tx0lqgff	31	25	primer	primer	NN
cord-271701-tx0lqgff	31	26	extension	extension	NN
cord-271701-tx0lqgff	31	27	.	.	.
cord-271701-tx0lqgff	32	1	For	for	IN
cord-271701-tx0lqgff	32	2	SARS	SARS	NNP
cord-271701-tx0lqgff	32	3	-	-	HYPH
cord-271701-tx0lqgff	32	4	CoV	CoV	NNP
cord-271701-tx0lqgff	32	5	nsp7-nsp8	nsp7-nsp8	CD
cord-271701-tx0lqgff	32	6	expression	expression	NN
cord-271701-tx0lqgff	32	7	,	,	,
cord-271701-tx0lqgff	32	8	the	the	DT
cord-271701-tx0lqgff	32	9	sequence	sequence	NN
cord-271701-tx0lqgff	32	10	encoding	encode	VBG
cord-271701-tx0lqgff	32	11	amino	amino	JJ
cord-271701-tx0lqgff	32	12	acids	acid	NNS
cord-271701-tx0lqgff	32	13	3837	3837	CD
cord-271701-tx0lqgff	32	14	-	-	:
cord-271701-tx0lqgff	32	15	4117	4117	CD
cord-271701-tx0lqgff	32	16	of	of	IN
cord-271701-tx0lqgff	32	17	the	the	DT
cord-271701-tx0lqgff	32	18	SARS	SARS	NNP
cord-271701-tx0lqgff	32	19	-	-	HYPH
cord-271701-tx0lqgff	32	20	CoV	CoV	NNP
cord-271701-tx0lqgff	32	21	replicase	replicase	NNP
cord-271701-tx0lqgff	32	22	pp1a	pp1a	NNP
cord-271701-tx0lqgff	32	23	was	be	VBD
cord-271701-tx0lqgff	32	24	amplified	amplify	VBN
cord-271701-tx0lqgff	32	25	by	by	IN
cord-271701-tx0lqgff	32	26	reverse	reverse	NN
cord-271701-tx0lqgff	32	27	transcriptionpolymerase	transcriptionpolymerase	NNP
cord-271701-tx0lqgff	32	28	chain	chain	NN
cord-271701-tx0lqgff	32	29	reaction	reaction	NN
cord-271701-tx0lqgff	32	30	(	(	-LRB-
cord-271701-tx0lqgff	32	31	RT	RT	NNP
cord-271701-tx0lqgff	32	32	-	-	HYPH
cord-271701-tx0lqgff	32	33	PCR	PCR	NNP
cord-271701-tx0lqgff	32	34	)	)	-RRB-
cord-271701-tx0lqgff	32	35	from	from	IN
cord-271701-tx0lqgff	32	36	the	the	DT
cord-271701-tx0lqgff	32	37	genome	genome	NN
cord-271701-tx0lqgff	32	38	of	of	IN
cord-271701-tx0lqgff	32	39	SARS	SARS	NNP
cord-271701-tx0lqgff	32	40	-	-	HYPH
cord-271701-tx0lqgff	32	41	CoV	CoV	NNP
cord-271701-tx0lqgff	32	42	isolate	isolate	NN
cord-271701-tx0lqgff	33	1	Frankfurt-1	Frankfurt-1	NNP
cord-271701-tx0lqgff	34	1	(	(	-LRB-
cord-271701-tx0lqgff	34	2	Genbank	Genbank	NNP
cord-271701-tx0lqgff	34	3	accession	accession	NN
cord-271701-tx0lqgff	34	4	number	number	NN
cord-271701-tx0lqgff	34	5	AY291315	ay291315	NN
cord-271701-tx0lqgff	34	6	)	)	-RRB-
cord-271701-tx0lqgff	34	7	.	.	.
cord-271701-tx0lqgff	35	1	The	the	DT
cord-271701-tx0lqgff	35	2	primers	primer	NNS
cord-271701-tx0lqgff	35	3	used	use	VBN
cord-271701-tx0lqgff	35	4	were	be	VBD
cord-271701-tx0lqgff	35	5	SAV704	sav704	JJ
cord-271701-tx0lqgff	35	6	and	and	CC
cord-271701-tx0lqgff	35	7	SAV429	sav429	JJ
cord-271701-tx0lqgff	35	8	(	(	-LRB-
cord-271701-tx0lqgff	35	9	Supplementary	supplementary	JJ
cord-271701-tx0lqgff	35	10	Table	Table	NNP
cord-271701-tx0lqgff	35	11	S1	S1	NNP
cord-271701-tx0lqgff	35	12	)	)	-RRB-
cord-271701-tx0lqgff	35	13	.	.	.
cord-271701-tx0lqgff	36	1	For	for	IN
cord-271701-tx0lqgff	36	2	nsp8	nsp8	JJ
cord-271701-tx0lqgff	36	3	expression	expression	NN
cord-271701-tx0lqgff	36	4	,	,	,
cord-271701-tx0lqgff	36	5	the	the	DT
cord-271701-tx0lqgff	36	6	sequence	sequence	NN
cord-271701-tx0lqgff	36	7	encoding	encode	VBG
cord-271701-tx0lqgff	37	1	pp1a	pp1a	NN
cord-271701-tx0lqgff	37	2	residues	residue	NNS
cord-271701-tx0lqgff	37	3	3920	3920	CD
cord-271701-tx0lqgff	37	4	to	to	IN
cord-271701-tx0lqgff	37	5	4117	4117	CD
cord-271701-tx0lqgff	37	6	was	be	VBD
cord-271701-tx0lqgff	37	7	amplified	amplify	VBN
cord-271701-tx0lqgff	37	8	by	by	IN
cord-271701-tx0lqgff	37	9	RT	RT	NNP
cord-271701-tx0lqgff	37	10	-	-	HYPH
cord-271701-tx0lqgff	37	11	PCR	PCR	NNP
cord-271701-tx0lqgff	37	12	using	use	VBG
cord-271701-tx0lqgff	37	13	SAV428	SAV428	NNP
cord-271701-tx0lqgff	37	14	and	and	CC
cord-271701-tx0lqgff	37	15	SAV429	SAV429	NNP
cord-271701-tx0lqgff	37	16	as	as	IN
cord-271701-tx0lqgff	37	17	primers	primer	NNS
cord-271701-tx0lqgff	37	18	(	(	-LRB-
cord-271701-tx0lqgff	37	19	Supplementary	supplementary	JJ
cord-271701-tx0lqgff	37	20	Table	Table	NNP
cord-271701-tx0lqgff	37	21	S1	S1	NNP
cord-271701-tx0lqgff	37	22	)	)	-RRB-
cord-271701-tx0lqgff	37	23	.	.	.
cord-271701-tx0lqgff	38	1	Both	both	DT
cord-271701-tx0lqgff	38	2	PCR	PCR	NNP
cord-271701-tx0lqgff	38	3	products	product	NNS
cord-271701-tx0lqgff	38	4	were	be	VBD
cord-271701-tx0lqgff	38	5	digested	digest	VBN
cord-271701-tx0lqgff	38	6	with	with	IN
cord-271701-tx0lqgff	38	7	SacII	SacII	NNP
cord-271701-tx0lqgff	38	8	and	and	CC
cord-271701-tx0lqgff	38	9	BamHI	BamHI	NNP
cord-271701-tx0lqgff	38	10	,	,	,
cord-271701-tx0lqgff	38	11	and	and	CC
cord-271701-tx0lqgff	38	12	ligated	ligate	VBD
cord-271701-tx0lqgff	38	13	into	into	IN
cord-271701-tx0lqgff	38	14	expression	expression	NN
cord-271701-tx0lqgff	38	15	vector	vector	NN
cord-271701-tx0lqgff	38	16	pASK3-Ub	pASK3-Ub	NNP
cord-271701-tx0lqgff	38	17	-	-	HYPH
cord-271701-tx0lqgff	38	18	CHis	CHis	NNP
cord-271701-tx0lqgff	38	19	6	6	CD
cord-271701-tx0lqgff	38	20	(	(	-LRB-
cord-271701-tx0lqgff	38	21	10	10	CD
cord-271701-tx0lqgff	38	22	)	)	-RRB-
cord-271701-tx0lqgff	38	23	.	.	.
cord-271701-tx0lqgff	39	1	This	this	DT
cord-271701-tx0lqgff	39	2	vector	vector	NN
cord-271701-tx0lqgff	39	3	was	be	VBD
cord-271701-tx0lqgff	39	4	originally	originally	RB
cord-271701-tx0lqgff	39	5	derived	derive	VBN
cord-271701-tx0lqgff	39	6	from	from	IN
cord-271701-tx0lqgff	39	7	the	the	DT
cord-271701-tx0lqgff	39	8	pET26-Ub	pET26-Ub	NNS
cord-271701-tx0lqgff	39	9	-	-	:
cord-271701-tx0lqgff	39	10	CHis	chis	$
cord-271701-tx0lqgff	39	11	6	6	CD
cord-271701-tx0lqgff	39	12	vector	vector	NN
cord-271701-tx0lqgff	39	13	(	(	-LRB-
cord-271701-tx0lqgff	39	14	14	14	CD
cord-271701-tx0lqgff	39	15	)	)	-RRB-
cord-271701-tx0lqgff	39	16	,	,	,
cord-271701-tx0lqgff	39	17	but	but	CC
cord-271701-tx0lqgff	39	18	drives	drive	VBZ
cord-271701-tx0lqgff	39	19	expression	expression	NN
cord-271701-tx0lqgff	39	20	of	of	IN
cord-271701-tx0lqgff	39	21	N	n	NN
cord-271701-tx0lqgff	39	22	-	-	HYPH
cord-271701-tx0lqgff	39	23	terminally	terminally	RB
cord-271701-tx0lqgff	39	24	ubiquitintagged	ubiquitintagge	VBN
cord-271701-tx0lqgff	39	25	and	and	CC
cord-271701-tx0lqgff	40	1	C	c	NN
cord-271701-tx0lqgff	40	2	-	-	:
cord-271701-tx0lqgff	40	3	terminally	terminally	RB
cord-271701-tx0lqgff	40	4	His	-PRON-	PRP$
cord-271701-tx0lqgff	40	5	6	6	CD
cord-271701-tx0lqgff	40	6	-tagged	-tagged	CD
cord-271701-tx0lqgff	40	7	fusion	fusion	NN
cord-271701-tx0lqgff	40	8	proteins	protein	NNS
cord-271701-tx0lqgff	40	9	via	via	IN
cord-271701-tx0lqgff	40	10	a	a	DT
cord-271701-tx0lqgff	40	11	tetracyclin	tetracyclin	JJ
cord-271701-tx0lqgff	40	12	-	-	HYPH
cord-271701-tx0lqgff	40	13	inducible	inducible	JJ
cord-271701-tx0lqgff	40	14	promoter	promoter	NN
cord-271701-tx0lqgff	40	15	,	,	,
cord-271701-tx0lqgff	40	16	to	to	TO
cord-271701-tx0lqgff	40	17	rule	rule	VB
cord-271701-tx0lqgff	40	18	out	out	RP
cord-271701-tx0lqgff	40	19	the	the	DT
cord-271701-tx0lqgff	40	20	potential	potential	JJ
cord-271701-tx0lqgff	40	21	T7	t7	NN
cord-271701-tx0lqgff	40	22	polymerase	polymerase	NN
cord-271701-tx0lqgff	40	23	contaminations	contamination	NNS
cord-271701-tx0lqgff	40	24	that	that	WDT
cord-271701-tx0lqgff	40	25	are	be	VBP
cord-271701-tx0lqgff	40	26	known	know	VBN
cord-271701-tx0lqgff	40	27	to	to	TO
cord-271701-tx0lqgff	40	28	cause	cause	VB
cord-271701-tx0lqgff	40	29	false	false	JJ
cord-271701-tx0lqgff	40	30	positive	positive	JJ
cord-271701-tx0lqgff	40	31	results	result	NNS
cord-271701-tx0lqgff	40	32	when	when	WRB
cord-271701-tx0lqgff	40	33	using	use	VBG
cord-271701-tx0lqgff	40	34	T7	t7	NN
cord-271701-tx0lqgff	40	35	promoter	promoter	NN
cord-271701-tx0lqgff	40	36	-	-	HYPH
cord-271701-tx0lqgff	40	37	driven	drive	VBN
cord-271701-tx0lqgff	40	38	systems	system	NNS
cord-271701-tx0lqgff	40	39	for	for	IN
cord-271701-tx0lqgff	40	40	recombinant	recombinant	JJ
cord-271701-tx0lqgff	40	41	RdRp	RdRp	NNP
cord-271701-tx0lqgff	40	42	expression	expression	NN
cord-271701-tx0lqgff	40	43	.	.	.
cord-271701-tx0lqgff	41	1	All	all	DT
cord-271701-tx0lqgff	41	2	described	describe	VBD
cord-271701-tx0lqgff	41	3	nsp8	nsp8	JJ
cord-271701-tx0lqgff	41	4	mutants	mutant	NNS
cord-271701-tx0lqgff	41	5	were	be	VBD
cord-271701-tx0lqgff	41	6	engineered	engineer	VBN
cord-271701-tx0lqgff	41	7	via	via	IN
cord-271701-tx0lqgff	41	8	site	site	NN
cord-271701-tx0lqgff	41	9	-	-	HYPH
cord-271701-tx0lqgff	41	10	directed	direct	VBN
cord-271701-tx0lqgff	41	11	mutagenesis	mutagenesis	NN
cord-271701-tx0lqgff	41	12	according	accord	VBG
cord-271701-tx0lqgff	41	13	to	to	IN
cord-271701-tx0lqgff	41	14	the	the	DT
cord-271701-tx0lqgff	41	15	QuikChange	QuikChange	NNP
cord-271701-tx0lqgff	41	16	protocol	protocol	NN
cord-271701-tx0lqgff	41	17	(	(	-LRB-
cord-271701-tx0lqgff	41	18	Stratagene	Stratagene	NNP
cord-271701-tx0lqgff	41	19	)	)	-RRB-
cord-271701-tx0lqgff	41	20	using	use	VBG
cord-271701-tx0lqgff	41	21	the	the	DT
cord-271701-tx0lqgff	41	22	primers	primer	NNS
cord-271701-tx0lqgff	41	23	listed	list	VBN
cord-271701-tx0lqgff	41	24	in	in	IN
cord-271701-tx0lqgff	41	25	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	41	26	Table	Table	NNP
cord-271701-tx0lqgff	41	27	S2	s2	NN
cord-271701-tx0lqgff	41	28	.	.	.
cord-271701-tx0lqgff	42	1	For	for	IN
cord-271701-tx0lqgff	42	2	nsp7	nsp7	JJ
cord-271701-tx0lqgff	42	3	-	-	SYM
cord-271701-tx0lqgff	42	4	8	8	CD
cord-271701-tx0lqgff	42	5	or	or	CC
cord-271701-tx0lqgff	42	6	nsp8	nsp8	JJ
cord-271701-tx0lqgff	42	7	expression	expression	NN
cord-271701-tx0lqgff	42	8	,	,	,
cord-271701-tx0lqgff	42	9	Escherichia	Escherichia	NNP
cord-271701-tx0lqgff	42	10	coli	coli	NNS
cord-271701-tx0lqgff	43	1	C2523	C2523	NNP
cord-271701-tx0lqgff	43	2	cells	cell	NNS
cord-271701-tx0lqgff	43	3	(	(	-LRB-
cord-271701-tx0lqgff	43	4	New	New	NNP
cord-271701-tx0lqgff	43	5	England	England	NNP
cord-271701-tx0lqgff	43	6	Biolabs	Biolabs	NNP
cord-271701-tx0lqgff	43	7	)	)	-RRB-
cord-271701-tx0lqgff	43	8	were	be	VBD
cord-271701-tx0lqgff	43	9	transformed	transform	VBN
cord-271701-tx0lqgff	43	10	with	with	IN
cord-271701-tx0lqgff	43	11	the	the	DT
cord-271701-tx0lqgff	43	12	plasmids	plasmid	NNS
cord-271701-tx0lqgff	44	1	pASK3-Ub	pASK3-Ub	NNP
cord-271701-tx0lqgff	44	2	-	-	HYPH
cord-271701-tx0lqgff	44	3	nsp7	nsp7	NN
cord-271701-tx0lqgff	44	4	-	-	HYPH
cord-271701-tx0lqgff	44	5	8-CHis	8-chis	CD
cord-271701-tx0lqgff	44	6	6	6	CD
cord-271701-tx0lqgff	44	7	or	or	CC
cord-271701-tx0lqgff	44	8	pASK3-Ub	pASK3-Ub	NNP
cord-271701-tx0lqgff	44	9	-	-	HYPH
cord-271701-tx0lqgff	44	10	nsp8-CHis	nsp8-chis	NN
cord-271701-tx0lqgff	44	11	6	6	CD
cord-271701-tx0lqgff	44	12	together	together	RB
cord-271701-tx0lqgff	44	13	with	with	IN
cord-271701-tx0lqgff	44	14	the	the	DT
cord-271701-tx0lqgff	44	15	Ubp1	ubp1	JJ
cord-271701-tx0lqgff	44	16	protease	protease	NN
cord-271701-tx0lqgff	44	17	expression	expression	NN
cord-271701-tx0lqgff	44	18	plasmid	plasmid	NN
cord-271701-tx0lqgff	44	19	pCG1	pCG1	NNP
cord-271701-tx0lqgff	44	20	(	(	-LRB-
cord-271701-tx0lqgff	44	21	14	14	CD
cord-271701-tx0lqgff	44	22	)	)	-RRB-
cord-271701-tx0lqgff	44	23	.	.	.
cord-271701-tx0lqgff	45	1	Routinely	routinely	RB
cord-271701-tx0lqgff	45	2	,	,	,
cord-271701-tx0lqgff	45	3	50	50	CD
cord-271701-tx0lqgff	45	4	ml	ml	NNS
cord-271701-tx0lqgff	45	5	of	of	IN
cord-271701-tx0lqgff	45	6	Luria	Luria	NNP
cord-271701-tx0lqgff	45	7	Broth	Broth	NNP
cord-271701-tx0lqgff	45	8	,	,	,
cord-271701-tx0lqgff	45	9	containing	contain	VBG
cord-271701-tx0lqgff	45	10	ampicillin	ampicillin	NN
cord-271701-tx0lqgff	45	11	(	(	-LRB-
cord-271701-tx0lqgff	45	12	50	50	CD
cord-271701-tx0lqgff	45	13	mg	mg	NNP
cord-271701-tx0lqgff	45	14	/	/	SYM
cord-271701-tx0lqgff	45	15	ml	ml	NNP
cord-271701-tx0lqgff	45	16	)	)	-RRB-
cord-271701-tx0lqgff	45	17	and	and	CC
cord-271701-tx0lqgff	45	18	chloramphenicol	chloramphenicol	NNP
cord-271701-tx0lqgff	45	19	(	(	-LRB-
cord-271701-tx0lqgff	45	20	34	34	CD
cord-271701-tx0lqgff	45	21	mg	mg	NNP
cord-271701-tx0lqgff	45	22	/	/	SYM
cord-271701-tx0lqgff	45	23	ml	ml	NNP
cord-271701-tx0lqgff	45	24	)	)	-RRB-
cord-271701-tx0lqgff	45	25	,	,	,
cord-271701-tx0lqgff	45	26	was	be	VBD
cord-271701-tx0lqgff	45	27	inoculated	inoculate	VBN
cord-271701-tx0lqgff	45	28	1:1000	1:1000	CD
cord-271701-tx0lqgff	46	1	with	with	IN
cord-271701-tx0lqgff	46	2	o	o	NNP
cord-271701-tx0lqgff	46	3	/	/	SYM
cord-271701-tx0lqgff	46	4	n	n	CC
cord-271701-tx0lqgff	46	5	precultures	preculture	NNS
cord-271701-tx0lqgff	46	6	,	,	,
cord-271701-tx0lqgff	46	7	and	and	CC
cord-271701-tx0lqgff	46	8	cells	cell	NNS
cord-271701-tx0lqgff	46	9	were	be	VBD
cord-271701-tx0lqgff	46	10	grown	grow	VBN
cord-271701-tx0lqgff	46	11	to	to	IN
cord-271701-tx0lqgff	46	12	OD	OD	NNP
cord-271701-tx0lqgff	46	13	600	600	CD
cord-271701-tx0lqgff	46	14	>	>	XX
cord-271701-tx0lqgff	46	15	0.8	0.8	CD
cord-271701-tx0lqgff	46	16	at	at	IN
cord-271701-tx0lqgff	46	17	37	37	CD
cord-271701-tx0lqgff	46	18	C.	c.	NN
cord-271701-tx0lqgff	47	1	Subsequently	subsequently	RB
cord-271701-tx0lqgff	47	2	,	,	,
cord-271701-tx0lqgff	47	3	the	the	DT
cord-271701-tx0lqgff	47	4	cells	cell	NNS
cord-271701-tx0lqgff	47	5	were	be	VBD
cord-271701-tx0lqgff	47	6	slowly	slowly	RB
cord-271701-tx0lqgff	47	7	cooled	cool	VBN
cord-271701-tx0lqgff	47	8	to	to	IN
cord-271701-tx0lqgff	47	9	20	20	CD
cord-271701-tx0lqgff	47	10	C	c	NN
cord-271701-tx0lqgff	47	11	,	,	,
cord-271701-tx0lqgff	47	12	followed	follow	VBN
cord-271701-tx0lqgff	47	13	by	by	IN
cord-271701-tx0lqgff	47	14	induction	induction	NN
cord-271701-tx0lqgff	47	15	with	with	IN
cord-271701-tx0lqgff	47	16	anhydrotetracycline	anhydrotetracycline	NN
cord-271701-tx0lqgff	47	17	(	(	-LRB-
cord-271701-tx0lqgff	47	18	Fluka	Fluka	NNP
cord-271701-tx0lqgff	47	19	)	)	-RRB-
cord-271701-tx0lqgff	47	20	at	at	IN
cord-271701-tx0lqgff	47	21	a	a	DT
cord-271701-tx0lqgff	47	22	final	final	JJ
cord-271701-tx0lqgff	47	23	concentration	concentration	NN
cord-271701-tx0lqgff	47	24	of	of	IN
cord-271701-tx0lqgff	47	25	200	200	CD
cord-271701-tx0lqgff	47	26	ng	ng	NN
cord-271701-tx0lqgff	47	27	/	/	SYM
cord-271701-tx0lqgff	47	28	ml	ml	NN
cord-271701-tx0lqgff	47	29	for	for	IN
cord-271701-tx0lqgff	47	30	16	16	CD
cord-271701-tx0lqgff	47	31	h.	h.	NN
cord-271701-tx0lqgff	47	32	Expression	expression	NN
cord-271701-tx0lqgff	47	33	at	at	IN
cord-271701-tx0lqgff	47	34	20	20	CD
cord-271701-tx0lqgff	47	35	C	c	NN
cord-271701-tx0lqgff	47	36	was	be	VBD
cord-271701-tx0lqgff	47	37	,	,	,
cord-271701-tx0lqgff	47	38	however	however	RB
cord-271701-tx0lqgff	47	39	,	,	,
cord-271701-tx0lqgff	47	40	only	only	RB
cord-271701-tx0lqgff	47	41	crucial	crucial	JJ
cord-271701-tx0lqgff	47	42	for	for	IN
cord-271701-tx0lqgff	47	43	the	the	DT
cord-271701-tx0lqgff	47	44	preparation	preparation	NN
cord-271701-tx0lqgff	47	45	of	of	IN
cord-271701-tx0lqgff	47	46	certain	certain	JJ
cord-271701-tx0lqgff	47	47	nsp8	nsp8	JJ
cord-271701-tx0lqgff	47	48	mutants	mutant	NNS
cord-271701-tx0lqgff	47	49	and	and	CC
cord-271701-tx0lqgff	47	50	similar	similar	JJ
cord-271701-tx0lqgff	47	51	yields	yield	NNS
cord-271701-tx0lqgff	47	52	of	of	IN
cord-271701-tx0lqgff	47	53	active	active	JJ
cord-271701-tx0lqgff	47	54	wild	wild	JJ
cord-271701-tx0lqgff	47	55	-	-	HYPH
cord-271701-tx0lqgff	47	56	type	type	NN
cord-271701-tx0lqgff	47	57	protein	protein	NN
cord-271701-tx0lqgff	47	58	could	could	MD
cord-271701-tx0lqgff	47	59	be	be	VB
cord-271701-tx0lqgff	47	60	obtained	obtain	VBN
cord-271701-tx0lqgff	47	61	by	by	IN
cord-271701-tx0lqgff	47	62	expression	expression	NN
cord-271701-tx0lqgff	47	63	at	at	IN
cord-271701-tx0lqgff	47	64	37	37	CD
cord-271701-tx0lqgff	47	65	C	c	NN
cord-271701-tx0lqgff	47	66	for	for	IN
cord-271701-tx0lqgff	47	67	3	3	CD
cord-271701-tx0lqgff	47	68	-	-	SYM
cord-271701-tx0lqgff	47	69	4	4	CD
cord-271701-tx0lqgff	47	70	h.	h.	NNP
cord-271701-tx0lqgff	47	71	Cells	cell	NNS
cord-271701-tx0lqgff	47	72	were	be	VBD
cord-271701-tx0lqgff	47	73	harvested	harvest	VBN
cord-271701-tx0lqgff	47	74	by	by	IN
cord-271701-tx0lqgff	47	75	centrifugation	centrifugation	NN
cord-271701-tx0lqgff	47	76	and	and	CC
cord-271701-tx0lqgff	47	77	stored	store	VBN
cord-271701-tx0lqgff	47	78	at	at	IN
cord-271701-tx0lqgff	47	79	20	20	CD
cord-271701-tx0lqgff	47	80	C	c	NN
cord-271701-tx0lqgff	47	81	until	until	IN
cord-271701-tx0lqgff	47	82	protein	protein	NN
cord-271701-tx0lqgff	47	83	purification	purification	NN
cord-271701-tx0lqgff	47	84	was	be	VBD
cord-271701-tx0lqgff	47	85	started	start	VBN
cord-271701-tx0lqgff	47	86	.	.	.
cord-271701-tx0lqgff	48	1	The	the	DT
cord-271701-tx0lqgff	48	2	expression	expression	NN
cord-271701-tx0lqgff	48	3	of	of	IN
cord-271701-tx0lqgff	48	4	SARS	SARS	NNP
cord-271701-tx0lqgff	48	5	-	-	HYPH
cord-271701-tx0lqgff	48	6	CoV	CoV	NNP
cord-271701-tx0lqgff	48	7	nsp7	nsp7	NN
cord-271701-tx0lqgff	48	8	with	with	IN
cord-271701-tx0lqgff	48	9	a	a	DT
cord-271701-tx0lqgff	48	10	C	c	NN
cord-271701-tx0lqgff	48	11	-	-	HYPH
cord-271701-tx0lqgff	48	12	terminal	terminal	NN
cord-271701-tx0lqgff	49	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	49	2	6	6	CD
cord-271701-tx0lqgff	49	3	-tag	-tag	:
cord-271701-tx0lqgff	49	4	(	(	-LRB-
cord-271701-tx0lqgff	49	5	nsp7-His	nsp7-his	NN
cord-271701-tx0lqgff	49	6	)	)	-RRB-
cord-271701-tx0lqgff	49	7	was	be	VBD
cord-271701-tx0lqgff	49	8	achieved	achieve	VBN
cord-271701-tx0lqgff	49	9	from	from	IN
cord-271701-tx0lqgff	49	10	plasmid	plasmid	NN
cord-271701-tx0lqgff	49	11	pDEST14-nsp7-His	pDEST14-nsp7-His	NNP
cord-271701-tx0lqgff	49	12	6	6	CD
cord-271701-tx0lqgff	49	13	according	accord	VBG
cord-271701-tx0lqgff	49	14	to	to	IN
cord-271701-tx0lqgff	49	15	the	the	DT
cord-271701-tx0lqgff	49	16	protocol	protocol	NN
cord-271701-tx0lqgff	49	17	previously	previously	RB
cord-271701-tx0lqgff	49	18	described	describe	VBN
cord-271701-tx0lqgff	49	19	for	for	IN
cord-271701-tx0lqgff	49	20	EAV	EAV	NNP
cord-271701-tx0lqgff	49	21	nsp9	nsp9	NNP
cord-271701-tx0lqgff	49	22	(	(	-LRB-
cord-271701-tx0lqgff	49	23	11	11	CD
cord-271701-tx0lqgff	49	24	)	)	-RRB-
cord-271701-tx0lqgff	49	25	.	.	.
cord-271701-tx0lqgff	50	1	SARS	SARS	NNP
cord-271701-tx0lqgff	50	2	-	-	HYPH
cord-271701-tx0lqgff	50	3	CoV	CoV	NNP
cord-271701-tx0lqgff	50	4	nsp5-His	nsp5-His	NNP
cord-271701-tx0lqgff	50	5	6	6	CD
cord-271701-tx0lqgff	50	6	(	(	-LRB-
cord-271701-tx0lqgff	50	7	nsp5-His	nsp5-His	NNP
cord-271701-tx0lqgff	50	8	)	)	-RRB-
cord-271701-tx0lqgff	50	9	was	be	VBD
cord-271701-tx0lqgff	50	10	expressed	express	VBN
cord-271701-tx0lqgff	50	11	as	as	IN
cord-271701-tx0lqgff	50	12	a	a	DT
cord-271701-tx0lqgff	50	13	self	self	NN
cord-271701-tx0lqgff	50	14	-	-	HYPH
cord-271701-tx0lqgff	50	15	cleaving	cleave	VBG
cord-271701-tx0lqgff	50	16	maltose	maltose	NN
cord-271701-tx0lqgff	50	17	binding	bind	VBG
cord-271701-tx0lqgff	50	18	protein	protein	NN
cord-271701-tx0lqgff	50	19	(	(	-LRB-
cord-271701-tx0lqgff	50	20	MBP)-fusion	MBP)-fusion	VBG
cord-271701-tx0lqgff	50	21	protein	protein	NN
cord-271701-tx0lqgff	50	22	and	and	CC
cord-271701-tx0lqgff	50	23	was	be	VBD
cord-271701-tx0lqgff	50	24	purified	purify	VBN
cord-271701-tx0lqgff	50	25	via	via	IN
cord-271701-tx0lqgff	50	26	its	-PRON-	PRP$
cord-271701-tx0lqgff	50	27	C	c	NN
cord-271701-tx0lqgff	50	28	-	-	HYPH
cord-271701-tx0lqgff	50	29	terminal	terminal	NN
cord-271701-tx0lqgff	51	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	51	2	6	6	CD
cord-271701-tx0lqgff	51	3	-tag	-tag	:
cord-271701-tx0lqgff	51	4	(	(	-LRB-
cord-271701-tx0lqgff	51	5	15	15	CD
cord-271701-tx0lqgff	51	6	)	)	-RRB-
cord-271701-tx0lqgff	51	7	.	.	.
cord-271701-tx0lqgff	52	1	The	the	DT
cord-271701-tx0lqgff	52	2	pASK3-His	pask3-his	CD
cord-271701-tx0lqgff	52	3	-	-	HYPH
cord-271701-tx0lqgff	52	4	nsp8	nsp8	JJ
cord-271701-tx0lqgff	52	5	plasmid	plasmid	NN
cord-271701-tx0lqgff	52	6	for	for	IN
cord-271701-tx0lqgff	52	7	expression	expression	NN
cord-271701-tx0lqgff	52	8	of	of	IN
cord-271701-tx0lqgff	52	9	the	the	DT
cord-271701-tx0lqgff	52	10	N	n	NN
cord-271701-tx0lqgff	52	11	-	-	HYPH
cord-271701-tx0lqgff	52	12	terminally	terminally	RB
cord-271701-tx0lqgff	52	13	His	-PRON-	PRP$
cord-271701-tx0lqgff	52	14	6	6	CD
cord-271701-tx0lqgff	52	15	-tagged	-tagged	CD
cord-271701-tx0lqgff	53	1	The	the	DT
cord-271701-tx0lqgff	53	2	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	53	3	genome	genome	NN
cord-271701-tx0lqgff	53	4	contains	contain	VBZ
cord-271701-tx0lqgff	53	5	two	two	CD
cord-271701-tx0lqgff	53	6	large	large	JJ
cord-271701-tx0lqgff	53	7	5	5	CD
cord-271701-tx0lqgff	53	8	0	0	CD
cord-271701-tx0lqgff	53	9	-proximal	-proximal	CD
cord-271701-tx0lqgff	53	10	ORFs	ORFs	NNP
cord-271701-tx0lqgff	53	11	(	(	-LRB-
cord-271701-tx0lqgff	53	12	ORF1a	ORF1a	NNP
cord-271701-tx0lqgff	53	13	and	and	CC
cord-271701-tx0lqgff	53	14	1	1	CD
cord-271701-tx0lqgff	53	15	b	b	NN
cord-271701-tx0lqgff	53	16	)	)	-RRB-
cord-271701-tx0lqgff	53	17	that	that	WDT
cord-271701-tx0lqgff	53	18	encode	encode	VBP
cord-271701-tx0lqgff	53	19	the	the	DT
cord-271701-tx0lqgff	53	20	two	two	CD
cord-271701-tx0lqgff	53	21	replicase	replicase	NN
cord-271701-tx0lqgff	53	22	polyproteins	polyprotein	NNS
cord-271701-tx0lqgff	53	23	,	,	,
cord-271701-tx0lqgff	53	24	whose	whose	WP$
cord-271701-tx0lqgff	53	25	mature	mature	JJ
cord-271701-tx0lqgff	53	26	products	product	NNS
cord-271701-tx0lqgff	53	27	assemble	assemble	VB
cord-271701-tx0lqgff	53	28	into	into	IN
cord-271701-tx0lqgff	53	29	the	the	DT
cord-271701-tx0lqgff	53	30	viral	viral	JJ
cord-271701-tx0lqgff	53	31	replication	replication	NN
cord-271701-tx0lqgff	53	32	and	and	CC
cord-271701-tx0lqgff	53	33	transcription	transcription	NN
cord-271701-tx0lqgff	53	34	complex	complex	NN
cord-271701-tx0lqgff	53	35	.	.	.
cord-271701-tx0lqgff	54	1	Both	both	DT
cord-271701-tx0lqgff	54	2	polyproteins	polyprotein	NNS
cord-271701-tx0lqgff	54	3	are	be	VBP
cord-271701-tx0lqgff	54	4	cleaved	cleave	VBN
cord-271701-tx0lqgff	54	5	(	(	-LRB-
cord-271701-tx0lqgff	54	6	cleavage	cleavage	NN
cord-271701-tx0lqgff	54	7	sites	site	NNS
cord-271701-tx0lqgff	54	8	indicated	indicate	VBN
cord-271701-tx0lqgff	54	9	with	with	IN
cord-271701-tx0lqgff	54	10	arrow	arrow	NN
cord-271701-tx0lqgff	54	11	heads	head	NNS
cord-271701-tx0lqgff	54	12	)	)	-RRB-
cord-271701-tx0lqgff	54	13	by	by	IN
cord-271701-tx0lqgff	54	14	the	the	DT
cord-271701-tx0lqgff	54	15	proteinase	proteinase	NN
cord-271701-tx0lqgff	54	16	activities	activity	NNS
cord-271701-tx0lqgff	54	17	of	of	IN
cord-271701-tx0lqgff	54	18	nsp3	nsp3	NNS
cord-271701-tx0lqgff	54	19	(	(	-LRB-
cord-271701-tx0lqgff	54	20	black	black	JJ
cord-271701-tx0lqgff	54	21	lines	line	NNS
cord-271701-tx0lqgff	54	22	)	)	-RRB-
cord-271701-tx0lqgff	54	23	and	and	CC
cord-271701-tx0lqgff	54	24	nsp5	nsp5	NNP
cord-271701-tx0lqgff	54	25	(	(	-LRB-
cord-271701-tx0lqgff	54	26	red	red	JJ
cord-271701-tx0lqgff	54	27	lines	line	NNS
cord-271701-tx0lqgff	54	28	)	)	-RRB-
cord-271701-tx0lqgff	54	29	,	,	,
cord-271701-tx0lqgff	54	30	which	which	WDT
cord-271701-tx0lqgff	54	31	releases	release	VBZ
cord-271701-tx0lqgff	54	32	the	the	DT
cord-271701-tx0lqgff	54	33	mature	mature	JJ
cord-271701-tx0lqgff	54	34	nsps	nsp	NNS
cord-271701-tx0lqgff	54	35	.	.	.
cord-271701-tx0lqgff	55	1	Also	also	RB
cord-271701-tx0lqgff	55	2	indicated	indicate	VBN
cord-271701-tx0lqgff	55	3	are	be	VBP
cord-271701-tx0lqgff	55	4	the	the	DT
cord-271701-tx0lqgff	55	5	5	5	CD
cord-271701-tx0lqgff	55	6	0	0	CD
cord-271701-tx0lqgff	55	7	cap	cap	NN
cord-271701-tx0lqgff	55	8	structure	structure	NN
cord-271701-tx0lqgff	55	9	and	and	CC
cord-271701-tx0lqgff	55	10	the	the	DT
cord-271701-tx0lqgff	55	11	3	3	CD
cord-271701-tx0lqgff	55	12	0	0	CD
cord-271701-tx0lqgff	55	13	polyA	polyA	NNP
cord-271701-tx0lqgff	55	14	tail	tail	NN
cord-271701-tx0lqgff	55	15	(	(	-LRB-
cord-271701-tx0lqgff	55	16	A	a	NN
cord-271701-tx0lqgff	55	17	n	n	NN
cord-271701-tx0lqgff	55	18	)	)	-RRB-
cord-271701-tx0lqgff	55	19	.	.	.
cord-271701-tx0lqgff	56	1	(	(	-LRB-
cord-271701-tx0lqgff	56	2	B	b	NN
cord-271701-tx0lqgff	56	3	)	)	-RRB-
cord-271701-tx0lqgff	57	1	The	the	DT
cord-271701-tx0lqgff	57	2	SARS	SARS	NNP
cord-271701-tx0lqgff	57	3	-	-	HYPH
cord-271701-tx0lqgff	57	4	CoV	CoV	NNP
cord-271701-tx0lqgff	57	5	nsp8	nsp8	NNP
cord-271701-tx0lqgff	57	6	crystal	crystal	NNP
cord-271701-tx0lqgff	57	7	structure	structure	NN
cord-271701-tx0lqgff	57	8	(	(	-LRB-
cord-271701-tx0lqgff	57	9	pdb	pdb	NNP
cord-271701-tx0lqgff	57	10	2AHM	2ahm	CD
cord-271701-tx0lqgff	57	11	)	)	-RRB-
cord-271701-tx0lqgff	57	12	resembles	resemble	VBZ
cord-271701-tx0lqgff	57	13	a	a	DT
cord-271701-tx0lqgff	57	14	'	'	``
cord-271701-tx0lqgff	57	15	golf	golf	NN
cord-271701-tx0lqgff	57	16	club	club	NN
cord-271701-tx0lqgff	57	17	-	-	HYPH
cord-271701-tx0lqgff	57	18	like	like	JJ
cord-271701-tx0lqgff	57	19	'	'	''
cord-271701-tx0lqgff	57	20	shape	shape	NN
cord-271701-tx0lqgff	57	21	,	,	,
cord-271701-tx0lqgff	57	22	as	as	IN
cord-271701-tx0lqgff	57	23	presented	present	VBN
cord-271701-tx0lqgff	57	24	by	by	IN
cord-271701-tx0lqgff	57	25	the	the	DT
cord-271701-tx0lqgff	57	26	yellow	yellow	JJ
cord-271701-tx0lqgff	57	27	ribbon	ribbon	NN
cord-271701-tx0lqgff	57	28	structure	structure	NN
cord-271701-tx0lqgff	57	29	.	.	.
cord-271701-tx0lqgff	58	1	This	this	DT
cord-271701-tx0lqgff	58	2	nsp8	nsp8	JJ
cord-271701-tx0lqgff	58	3	conformation	conformation	NN
cord-271701-tx0lqgff	58	4	connects	connect	VBZ
cord-271701-tx0lqgff	58	5	to	to	IN
cord-271701-tx0lqgff	58	6	a	a	DT
cord-271701-tx0lqgff	58	7	much	much	RB
cord-271701-tx0lqgff	58	8	larger	large	JJR
cord-271701-tx0lqgff	58	9	,	,	,
cord-271701-tx0lqgff	58	10	hexadecameric	hexadecameric	NNP
cord-271701-tx0lqgff	58	11	structure	structure	NN
cord-271701-tx0lqgff	58	12	that	that	WDT
cord-271701-tx0lqgff	58	13	is	be	VBZ
cord-271701-tx0lqgff	58	14	composed	compose	VBN
cord-271701-tx0lqgff	58	15	of	of	IN
cord-271701-tx0lqgff	58	16	seven	seven	CD
cord-271701-tx0lqgff	58	17	additional	additional	JJ
cord-271701-tx0lqgff	58	18	nsp8	nsp8	JJ
cord-271701-tx0lqgff	58	19	subunits	subunit	NNS
cord-271701-tx0lqgff	58	20	(	(	-LRB-
cord-271701-tx0lqgff	58	21	grey	grey	NNP
cord-271701-tx0lqgff	58	22	)	)	-RRB-
cord-271701-tx0lqgff	58	23	and	and	CC
cord-271701-tx0lqgff	58	24	eight	eight	CD
cord-271701-tx0lqgff	58	25	nsp7	nsp7	NNP
cord-271701-tx0lqgff	58	26	subunits	subunit	NNS
cord-271701-tx0lqgff	58	27	(	(	-LRB-
cord-271701-tx0lqgff	58	28	green	green	JJ
cord-271701-tx0lqgff	58	29	)	)	-RRB-
cord-271701-tx0lqgff	58	30	.	.	.
cord-271701-tx0lqgff	59	1	The	the	DT
cord-271701-tx0lqgff	59	2	hollow	hollow	JJ
cord-271701-tx0lqgff	59	3	hexadecameric	hexadecameric	NNP
cord-271701-tx0lqgff	59	4	ring	ring	NN
cord-271701-tx0lqgff	59	5	structure	structure	NN
cord-271701-tx0lqgff	59	6	has	have	VBZ
cord-271701-tx0lqgff	59	7	a	a	DT
cord-271701-tx0lqgff	59	8	positively	positively	RB
cord-271701-tx0lqgff	59	9	charged	charge	VBN
cord-271701-tx0lqgff	59	10	channel	channel	NN
cord-271701-tx0lqgff	59	11	(	(	-LRB-
cord-271701-tx0lqgff	59	12	blue	blue	JJ
cord-271701-tx0lqgff	59	13	background	background	NN
cord-271701-tx0lqgff	59	14	shading	shade	VBG
cord-271701-tx0lqgff	59	15	)	)	-RRB-
cord-271701-tx0lqgff	59	16	that	that	WDT
cord-271701-tx0lqgff	59	17	was	be	VBD
cord-271701-tx0lqgff	59	18	proposed	propose	VBN
cord-271701-tx0lqgff	59	19	to	to	TO
cord-271701-tx0lqgff	59	20	mediate	mediate	VB
cord-271701-tx0lqgff	59	21	RNA	RNA	NNP
cord-271701-tx0lqgff	59	22	binding	binding	NN
cord-271701-tx0lqgff	59	23	.	.	.
cord-271701-tx0lqgff	60	1	The	the	DT
cord-271701-tx0lqgff	60	2	outside	outside	NN
cord-271701-tx0lqgff	60	3	of	of	IN
cord-271701-tx0lqgff	60	4	the	the	DT
cord-271701-tx0lqgff	60	5	structure	structure	NN
cord-271701-tx0lqgff	60	6	is	be	VBZ
cord-271701-tx0lqgff	60	7	predominantly	predominantly	RB
cord-271701-tx0lqgff	60	8	negatively	negatively	RB
cord-271701-tx0lqgff	60	9	charged	charge	VBN
cord-271701-tx0lqgff	60	10	(	(	-LRB-
cord-271701-tx0lqgff	60	11	red	red	JJ
cord-271701-tx0lqgff	60	12	background	background	NN
cord-271701-tx0lqgff	60	13	shading	shade	VBG
cord-271701-tx0lqgff	60	14	)	)	-RRB-
cord-271701-tx0lqgff	60	15	.	.	.
cord-271701-tx0lqgff	61	1	nsp8	nsp8	NN
cord-271701-tx0lqgff	61	2	was	be	VBD
cord-271701-tx0lqgff	61	3	kindly	kindly	RB
cord-271701-tx0lqgff	61	4	provided	provide	VBN
cord-271701-tx0lqgff	61	5	by	by	IN
cord-271701-tx0lqgff	61	6	Dr	Dr	NNP
cord-271701-tx0lqgff	61	7	Imbert	Imbert	NNP
cord-271701-tx0lqgff	61	8	and	and	CC
cord-271701-tx0lqgff	61	9	Dr	Dr	NNP
cord-271701-tx0lqgff	61	10	Canard	Canard	NNP
cord-271701-tx0lqgff	62	1	(	(	-LRB-
cord-271701-tx0lqgff	62	2	University	University	NNP
cord-271701-tx0lqgff	62	3	of	of	IN
cord-271701-tx0lqgff	62	4	Marseille	Marseille	NNP
cord-271701-tx0lqgff	62	5	,	,	,
cord-271701-tx0lqgff	62	6	France	France	NNP
cord-271701-tx0lqgff	62	7	)	)	-RRB-
cord-271701-tx0lqgff	62	8	.	.	.
cord-271701-tx0lqgff	63	1	Purification	purification	NN
cord-271701-tx0lqgff	63	2	of	of	IN
cord-271701-tx0lqgff	63	3	SARS	SARS	NNP
cord-271701-tx0lqgff	63	4	-	-	HYPH
cord-271701-tx0lqgff	63	5	CoV	CoV	NNP
cord-271701-tx0lqgff	63	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	63	7	,	,	,
cord-271701-tx0lqgff	63	8	nsp7	nsp7	NNP
cord-271701-tx0lqgff	63	9	-	-	SYM
cord-271701-tx0lqgff	63	10	8	8	CD
cord-271701-tx0lqgff	63	11	and	and	CC
cord-271701-tx0lqgff	63	12	nsp7	nsp7	JJ
cord-271701-tx0lqgff	63	13	Bacterial	bacterial	JJ
cord-271701-tx0lqgff	63	14	pellets	pellet	NNS
cord-271701-tx0lqgff	63	15	were	be	VBD
cord-271701-tx0lqgff	63	16	thawed	thaw	VBN
cord-271701-tx0lqgff	63	17	on	on	IN
cord-271701-tx0lqgff	63	18	ice	ice	NN
cord-271701-tx0lqgff	63	19	,	,	,
cord-271701-tx0lqgff	63	20	resuspended	resuspend	VBN
cord-271701-tx0lqgff	63	21	in	in	IN
cord-271701-tx0lqgff	63	22	buffer	buffer	NN
cord-271701-tx0lqgff	64	1	A	a	DT
cord-271701-tx0lqgff	64	2	[	[	-LRB-
cord-271701-tx0lqgff	64	3	20	20	CD
cord-271701-tx0lqgff	64	4	mM	mm	NN
cord-271701-tx0lqgff	65	1	HEPES	HEPES	NNP
cord-271701-tx0lqgff	65	2	pH	pH	NNP
cord-271701-tx0lqgff	65	3	7.4	7.4	CD
cord-271701-tx0lqgff	65	4	,	,	,
cord-271701-tx0lqgff	65	5	10	10	CD
cord-271701-tx0lqgff	65	6	mM	mm	CD
cord-271701-tx0lqgff	65	7	imidazole	imidazole	NN
cord-271701-tx0lqgff	65	8	,	,	,
cord-271701-tx0lqgff	65	9	0.05	0.05	CD
cord-271701-tx0lqgff	65	10	%	%	NN
cord-271701-tx0lqgff	65	11	Tween-20	Tween-20	NNP
cord-271701-tx0lqgff	65	12	,	,	,
cord-271701-tx0lqgff	65	13	5	5	CD
cord-271701-tx0lqgff	65	14	mM	mm	CD
cord-271701-tx0lqgff	65	15	b	b	NN
cord-271701-tx0lqgff	65	16	-	-	HYPH
cord-271701-tx0lqgff	65	17	mercaptoethanol	mercaptoethanol	NNP
cord-271701-tx0lqgff	65	18	and	and	CC
cord-271701-tx0lqgff	65	19	EDTA	edta	NN
cord-271701-tx0lqgff	65	20	-	-	HYPH
cord-271701-tx0lqgff	65	21	free	free	JJ
cord-271701-tx0lqgff	65	22	protease	protease	NN
cord-271701-tx0lqgff	65	23	inhibitor	inhibitor	NN
cord-271701-tx0lqgff	65	24	cocktail	cocktail	NN
cord-271701-tx0lqgff	65	25	(	(	-LRB-
cord-271701-tx0lqgff	65	26	Roche	Roche	NNP
cord-271701-tx0lqgff	65	27	)	)	-RRB-
cord-271701-tx0lqgff	65	28	]	]	-RRB-
cord-271701-tx0lqgff	66	1	containing	contain	VBG
cord-271701-tx0lqgff	66	2	500	500	CD
cord-271701-tx0lqgff	66	3	mM	mm	CD
cord-271701-tx0lqgff	66	4	NaCl	nacl	NN
cord-271701-tx0lqgff	66	5	,	,	,
cord-271701-tx0lqgff	66	6	and	and	CC
cord-271701-tx0lqgff	66	7	lysed	lyse	VBN
cord-271701-tx0lqgff	66	8	by	by	IN
cord-271701-tx0lqgff	66	9	sonication	sonication	NN
cord-271701-tx0lqgff	66	10	.	.	.
cord-271701-tx0lqgff	67	1	The	the	DT
cord-271701-tx0lqgff	67	2	supernatant	supernatant	NN
cord-271701-tx0lqgff	67	3	was	be	VBD
cord-271701-tx0lqgff	67	4	cleared	clear	VBN
cord-271701-tx0lqgff	67	5	by	by	IN
cord-271701-tx0lqgff	67	6	ultracentrifugation	ultracentrifugation	NN
cord-271701-tx0lqgff	67	7	at	at	IN
cord-271701-tx0lqgff	67	8	20	20	CD
cord-271701-tx0lqgff	67	9	000	000	CD
cord-271701-tx0lqgff	67	10	g	g	NN
cord-271701-tx0lqgff	67	11	for	for	IN
cord-271701-tx0lqgff	67	12	30	30	CD
cord-271701-tx0lqgff	67	13	min	min	NN
cord-271701-tx0lqgff	67	14	and	and	CC
cord-271701-tx0lqgff	67	15	subsequently	subsequently	RB
cord-271701-tx0lqgff	67	16	incubated	incubate	VBN
cord-271701-tx0lqgff	67	17	with	with	IN
cord-271701-tx0lqgff	67	18	Talon	Talon	NNP
cord-271701-tx0lqgff	67	19	beads	bead	NNS
cord-271701-tx0lqgff	67	20	(	(	-LRB-
cord-271701-tx0lqgff	67	21	Clontech	Clontech	NNP
cord-271701-tx0lqgff	67	22	)	)	-RRB-
cord-271701-tx0lqgff	67	23	for	for	IN
cord-271701-tx0lqgff	67	24	2	2	CD
cord-271701-tx0lqgff	67	25	h	h	NN
cord-271701-tx0lqgff	67	26	at	at	IN
cord-271701-tx0lqgff	67	27	4	4	CD
cord-271701-tx0lqgff	67	28	C.	c.	NN
cord-271701-tx0lqgff	68	1	The	the	DT
cord-271701-tx0lqgff	68	2	beads	bead	NNS
cord-271701-tx0lqgff	68	3	were	be	VBD
cord-271701-tx0lqgff	68	4	washed	wash	VBN
cord-271701-tx0lqgff	68	5	four	four	CD
cord-271701-tx0lqgff	68	6	times	time	NNS
cord-271701-tx0lqgff	68	7	15	15	CD
cord-271701-tx0lqgff	68	8	min	min	NN
cord-271701-tx0lqgff	68	9	with	with	IN
cord-271701-tx0lqgff	68	10	20	20	CD
cord-271701-tx0lqgff	68	11	volumes	volume	NNS
cord-271701-tx0lqgff	68	12	of	of	IN
cord-271701-tx0lqgff	68	13	binding	bind	VBG
cord-271701-tx0lqgff	68	14	buffer	buffer	NN
cord-271701-tx0lqgff	68	15	.	.	.
cord-271701-tx0lqgff	69	1	Ultimately	ultimately	RB
cord-271701-tx0lqgff	69	2	,	,	,
cord-271701-tx0lqgff	69	3	the	the	DT
cord-271701-tx0lqgff	69	4	C	c	NN
cord-271701-tx0lqgff	69	5	-	-	,
cord-271701-tx0lqgff	69	6	terminally	terminally	RB
cord-271701-tx0lqgff	69	7	His	-PRON-	PRP$
cord-271701-tx0lqgff	69	8	6	6	CD
cord-271701-tx0lqgff	69	9	-tagged	-tagged	CD
cord-271701-tx0lqgff	69	10	proteins	protein	NNS
cord-271701-tx0lqgff	69	11	were	be	VBD
cord-271701-tx0lqgff	69	12	eluted	elute	VBN
cord-271701-tx0lqgff	69	13	with	with	IN
cord-271701-tx0lqgff	69	14	150	150	CD
cord-271701-tx0lqgff	69	15	mM	mm	CD
cord-271701-tx0lqgff	69	16	imidazole	imidazole	NN
cord-271701-tx0lqgff	69	17	in	in	IN
cord-271701-tx0lqgff	69	18	buffer	buffer	NN
cord-271701-tx0lqgff	69	19	A	A	NNP
cord-271701-tx0lqgff	69	20	containing	contain	VBG
cord-271701-tx0lqgff	69	21	150	150	CD
cord-271701-tx0lqgff	69	22	mM	mm	CD
cord-271701-tx0lqgff	69	23	NaCl	nacl	NN
cord-271701-tx0lqgff	69	24	,	,	,
cord-271701-tx0lqgff	69	25	or	or	CC
cord-271701-tx0lqgff	69	26	cleaved	cleave	VBN
cord-271701-tx0lqgff	69	27	off	off	IN
cord-271701-tx0lqgff	69	28	of	of	IN
cord-271701-tx0lqgff	69	29	the	the	DT
cord-271701-tx0lqgff	69	30	column	column	NN
cord-271701-tx0lqgff	69	31	during	during	IN
cord-271701-tx0lqgff	69	32	a	a	DT
cord-271701-tx0lqgff	69	33	3-h	3-h	CD
cord-271701-tx0lqgff	69	34	digestion	digestion	NN
cord-271701-tx0lqgff	69	35	with	with	IN
cord-271701-tx0lqgff	69	36	SARS	SARS	NNP
cord-271701-tx0lqgff	69	37	-	-	HYPH
cord-271701-tx0lqgff	69	38	CoV	CoV	NNP
cord-271701-tx0lqgff	69	39	nsp5	nsp5	NN
cord-271701-tx0lqgff	69	40	in	in	IN
cord-271701-tx0lqgff	69	41	the	the	DT
cord-271701-tx0lqgff	69	42	presence	presence	NN
cord-271701-tx0lqgff	69	43	of	of	IN
cord-271701-tx0lqgff	69	44	4	4	CD
cord-271701-tx0lqgff	69	45	mM	mm	CD
cord-271701-tx0lqgff	69	46	MgCl	MgCl	NNP
cord-271701-tx0lqgff	69	47	2	2	CD
cord-271701-tx0lqgff	69	48	.	.	.
cord-271701-tx0lqgff	70	1	The	the	DT
cord-271701-tx0lqgff	70	2	eluates	eluate	NNS
cord-271701-tx0lqgff	70	3	were	be	VBD
cord-271701-tx0lqgff	70	4	analysed	analyse	VBN
cord-271701-tx0lqgff	70	5	by	by	IN
cord-271701-tx0lqgff	70	6	sodium	sodium	NN
cord-271701-tx0lqgff	70	7	dodecyl	dodecyl	NNP
cord-271701-tx0lqgff	70	8	sulfate	sulfate	NN
cord-271701-tx0lqgff	70	9	polyacrylamide	polyacrylamide	NN
cord-271701-tx0lqgff	70	10	gel	gel	NN
cord-271701-tx0lqgff	70	11	electrophoresis	electrophoresis	NN
cord-271701-tx0lqgff	70	12	(	(	-LRB-
cord-271701-tx0lqgff	70	13	SDS	SDS	NNP
cord-271701-tx0lqgff	70	14	-	-	HYPH
cord-271701-tx0lqgff	70	15	PAGE	PAGE	NNP
cord-271701-tx0lqgff	70	16	)	)	-RRB-
cord-271701-tx0lqgff	70	17	and	and	CC
cord-271701-tx0lqgff	70	18	typically	typically	RB
cord-271701-tx0lqgff	70	19	found	find	VBN
cord-271701-tx0lqgff	70	20	to	to	TO
cord-271701-tx0lqgff	70	21	be	be	VB
cord-271701-tx0lqgff	70	22	>	>	$
cord-271701-tx0lqgff	70	23	90	90	CD
cord-271701-tx0lqgff	70	24	%	%	NN
cord-271701-tx0lqgff	70	25	pure	pure	JJ
cord-271701-tx0lqgff	70	26	.	.	.
cord-271701-tx0lqgff	71	1	Elution	Elution	NNP
cord-271701-tx0lqgff	71	2	fractions	fraction	NNS
cord-271701-tx0lqgff	71	3	containing	contain	VBG
cord-271701-tx0lqgff	71	4	nsp8-	nsp8-	CD
cord-271701-tx0lqgff	71	5	,	,	,
cord-271701-tx0lqgff	71	6	nsp7	nsp7	NNP
cord-271701-tx0lqgff	71	7	-	-	SYM
cord-271701-tx0lqgff	71	8	8	8	CD
cord-271701-tx0lqgff	71	9	or	or	CC
cord-271701-tx0lqgff	71	10	nsp7	nsp7	NNPS
cord-271701-tx0lqgff	71	11	were	be	VBD
cord-271701-tx0lqgff	71	12	subsequently	subsequently	RB
cord-271701-tx0lqgff	71	13	pooled	pool	VBN
cord-271701-tx0lqgff	71	14	,	,	,
cord-271701-tx0lqgff	71	15	dialysed	dialysed	JJ
cord-271701-tx0lqgff	71	16	,	,	,
cord-271701-tx0lqgff	71	17	stored	store	VBN
cord-271701-tx0lqgff	71	18	and	and	CC
cord-271701-tx0lqgff	71	19	analysed	analyse	VBN
cord-271701-tx0lqgff	71	20	as	as	IN
cord-271701-tx0lqgff	71	21	described	describe	VBN
cord-271701-tx0lqgff	71	22	previously	previously	RB
cord-271701-tx0lqgff	71	23	for	for	IN
cord-271701-tx0lqgff	71	24	SARS	SARS	NNP
cord-271701-tx0lqgff	71	25	-	-	HYPH
cord-271701-tx0lqgff	71	26	CoV	CoV	NNP
cord-271701-tx0lqgff	71	27	nsp12	nsp12	CD
cord-271701-tx0lqgff	71	28	(	(	-LRB-
cord-271701-tx0lqgff	71	29	10	10	CD
cord-271701-tx0lqgff	71	30	)	)	-RRB-
cord-271701-tx0lqgff	71	31	.	.	.
cord-271701-tx0lqgff	72	1	To	to	TO
cord-271701-tx0lqgff	72	2	study	study	VB
cord-271701-tx0lqgff	72	3	SARS	SARS	NNP
cord-271701-tx0lqgff	72	4	-	-	HYPH
cord-271701-tx0lqgff	72	5	CoV	CoV	NNP
cord-271701-tx0lqgff	72	6	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	72	7	+	+	SYM
cord-271701-tx0lqgff	72	8	8	8	CD
cord-271701-tx0lqgff	72	9	)	)	-RRB-
cord-271701-tx0lqgff	73	1	complex	complex	JJ
cord-271701-tx0lqgff	73	2	formation	formation	NN
cord-271701-tx0lqgff	73	3	,	,	,
cord-271701-tx0lqgff	73	4	different	different	JJ
cord-271701-tx0lqgff	73	5	nsp8	nsp8	NN
cord-271701-tx0lqgff	74	1	:	:	:
cord-271701-tx0lqgff	74	2	nsp7	nsp7	JJ
cord-271701-tx0lqgff	74	3	ratios	ratio	NNS
cord-271701-tx0lqgff	74	4	were	be	VBD
cord-271701-tx0lqgff	74	5	mixed	mix	VBN
cord-271701-tx0lqgff	74	6	in	in	IN
cord-271701-tx0lqgff	74	7	binding	bind	VBG
cord-271701-tx0lqgff	74	8	buffer	buffer	NN
cord-271701-tx0lqgff	74	9	(	(	-LRB-
cord-271701-tx0lqgff	74	10	20	20	CD
cord-271701-tx0lqgff	74	11	mM	mm	CD
cord-271701-tx0lqgff	74	12	HEPES	HEPES	NNP
cord-271701-tx0lqgff	74	13	pH	ph	NN
cord-271701-tx0lqgff	74	14	7.5	7.5	CD
cord-271701-tx0lqgff	74	15	,	,	,
cord-271701-tx0lqgff	74	16	50	50	CD
cord-271701-tx0lqgff	74	17	mM	mm	CD
cord-271701-tx0lqgff	74	18	NaCl	nacl	NN
cord-271701-tx0lqgff	74	19	,	,	,
cord-271701-tx0lqgff	74	20	5	5	CD
cord-271701-tx0lqgff	74	21	%	%	NN
cord-271701-tx0lqgff	74	22	glycerol	glycerol	NN
cord-271701-tx0lqgff	74	23	,	,	,
cord-271701-tx0lqgff	74	24	0.1	0.1	CD
cord-271701-tx0lqgff	74	25	%	%	NN
cord-271701-tx0lqgff	74	26	Triton	Triton	NNP
cord-271701-tx0lqgff	74	27	X-100	x-100	NN
cord-271701-tx0lqgff	74	28	and	and	CC
cord-271701-tx0lqgff	74	29	1	1	CD
cord-271701-tx0lqgff	74	30	mM	mm	CD
cord-271701-tx0lqgff	74	31	DTT	DTT	NNP
cord-271701-tx0lqgff	74	32	)	)	-RRB-
cord-271701-tx0lqgff	74	33	to	to	TO
cord-271701-tx0lqgff	74	34	give	give	VB
cord-271701-tx0lqgff	74	35	a	a	DT
cord-271701-tx0lqgff	74	36	final	final	JJ
cord-271701-tx0lqgff	74	37	reaction	reaction	NN
cord-271701-tx0lqgff	74	38	volume	volume	NN
cord-271701-tx0lqgff	74	39	of	of	IN
cord-271701-tx0lqgff	74	40	10	10	CD
cord-271701-tx0lqgff	74	41	ml	ml	NNS
cord-271701-tx0lqgff	74	42	.	.	.
cord-271701-tx0lqgff	75	1	The	the	DT
cord-271701-tx0lqgff	75	2	proteins	protein	NNS
cord-271701-tx0lqgff	75	3	were	be	VBD
cord-271701-tx0lqgff	75	4	pre	pre	JJ
cord-271701-tx0lqgff	75	5	-	-	JJ
cord-271701-tx0lqgff	75	6	incubated	incubate	VBN
cord-271701-tx0lqgff	75	7	for	for	IN
cord-271701-tx0lqgff	75	8	10	10	CD
cord-271701-tx0lqgff	75	9	min	min	NN
cord-271701-tx0lqgff	75	10	at	at	IN
cord-271701-tx0lqgff	75	11	20	20	CD
cord-271701-tx0lqgff	75	12	C	c	NN
cord-271701-tx0lqgff	75	13	,	,	,
cord-271701-tx0lqgff	75	14	after	after	IN
cord-271701-tx0lqgff	75	15	which	which	WDT
cord-271701-tx0lqgff	75	16	cross	cross	NN
cord-271701-tx0lqgff	75	17	-	-	NN
cord-271701-tx0lqgff	75	18	linking	linking	NN
cord-271701-tx0lqgff	75	19	was	be	VBD
cord-271701-tx0lqgff	75	20	initiated	initiate	VBN
cord-271701-tx0lqgff	75	21	through	through	IN
cord-271701-tx0lqgff	75	22	the	the	DT
cord-271701-tx0lqgff	75	23	addition	addition	NN
cord-271701-tx0lqgff	75	24	of	of	IN
cord-271701-tx0lqgff	75	25	0.5	0.5	CD
cord-271701-tx0lqgff	75	26	ml	ml	NNS
cord-271701-tx0lqgff	75	27	of	of	IN
cord-271701-tx0lqgff	75	28	a	a	DT
cord-271701-tx0lqgff	75	29	freshly	freshly	RB
cord-271701-tx0lqgff	75	30	prepared	prepare	VBN
cord-271701-tx0lqgff	75	31	2.5	2.5	CD
cord-271701-tx0lqgff	75	32	%	%	NN
cord-271701-tx0lqgff	75	33	glutaraldehyde	glutaraldehyde	NN
cord-271701-tx0lqgff	75	34	solution	solution	NN
cord-271701-tx0lqgff	75	35	.	.	.
cord-271701-tx0lqgff	76	1	The	the	DT
cord-271701-tx0lqgff	76	2	reactions	reaction	NNS
cord-271701-tx0lqgff	76	3	were	be	VBD
cord-271701-tx0lqgff	76	4	incubated	incubate	VBN
cord-271701-tx0lqgff	76	5	for	for	IN
cord-271701-tx0lqgff	76	6	a	a	DT
cord-271701-tx0lqgff	76	7	further	further	JJ
cord-271701-tx0lqgff	76	8	5	5	CD
cord-271701-tx0lqgff	76	9	min	min	NN
cord-271701-tx0lqgff	76	10	at	at	IN
cord-271701-tx0lqgff	76	11	30	30	CD
cord-271701-tx0lqgff	76	12	C	c	NN
cord-271701-tx0lqgff	76	13	and	and	CC
cord-271701-tx0lqgff	76	14	then	then	RB
cord-271701-tx0lqgff	76	15	terminated	terminate	VBD
cord-271701-tx0lqgff	76	16	with	with	IN
cord-271701-tx0lqgff	76	17	1	1	CD
cord-271701-tx0lqgff	76	18	ml	ml	NN
cord-271701-tx0lqgff	76	19	1	1	CD
cord-271701-tx0lqgff	76	20	M	M	NNP
cord-271701-tx0lqgff	76	21	Tris	Tris	NNP
cord-271701-tx0lqgff	76	22	pH	ph	NN
cord-271701-tx0lqgff	76	23	8.0	8.0	CD
cord-271701-tx0lqgff	76	24	.	.	.
cord-271701-tx0lqgff	77	1	Analysis	analysis	NN
cord-271701-tx0lqgff	77	2	of	of	IN
cord-271701-tx0lqgff	77	3	complex	complex	JJ
cord-271701-tx0lqgff	77	4	formation	formation	NN
cord-271701-tx0lqgff	77	5	was	be	VBD
cord-271701-tx0lqgff	77	6	performed	perform	VBN
cord-271701-tx0lqgff	77	7	on	on	IN
cord-271701-tx0lqgff	77	8	SDS	SDS	NNP
cord-271701-tx0lqgff	77	9	-	-	HYPH
cord-271701-tx0lqgff	77	10	PAGE	PAGE	NNP
cord-271701-tx0lqgff	77	11	gels	gel	NNS
cord-271701-tx0lqgff	77	12	,	,	,
cord-271701-tx0lqgff	77	13	which	which	WDT
cord-271701-tx0lqgff	77	14	were	be	VBD
cord-271701-tx0lqgff	77	15	stained	stain	VBN
cord-271701-tx0lqgff	77	16	with	with	IN
cord-271701-tx0lqgff	77	17	Coomassie	Coomassie	NNP
cord-271701-tx0lqgff	77	18	G-250	g-250	JJ
cord-271701-tx0lqgff	77	19	dye	dye	NN
cord-271701-tx0lqgff	77	20	.	.	.
cord-271701-tx0lqgff	78	1	A	a	DT
cord-271701-tx0lqgff	78	2	dilution	dilution	NN
cord-271701-tx0lqgff	78	3	series	series	NN
cord-271701-tx0lqgff	78	4	of	of	IN
cord-271701-tx0lqgff	78	5	0	0	CD
cord-271701-tx0lqgff	78	6	-	-	SYM
cord-271701-tx0lqgff	78	7	5	5	CD
cord-271701-tx0lqgff	78	8	mM	mM	NNP
cord-271701-tx0lqgff	78	9	SARS	SARS	NNP
cord-271701-tx0lqgff	78	10	-	-	HYPH
cord-271701-tx0lqgff	78	11	CoV	CoV	NNP
cord-271701-tx0lqgff	78	12	nsp8	nsp8	NN
cord-271701-tx0lqgff	78	13	in	in	IN
cord-271701-tx0lqgff	78	14	storage	storage	NN
cord-271701-tx0lqgff	78	15	buffer	buffer	NN
cord-271701-tx0lqgff	78	16	was	be	VBD
cord-271701-tx0lqgff	78	17	incubated	incubate	VBN
cord-271701-tx0lqgff	78	18	for	for	IN
cord-271701-tx0lqgff	78	19	10	10	CD
cord-271701-tx0lqgff	78	20	min	min	NN
cord-271701-tx0lqgff	78	21	at	at	IN
cord-271701-tx0lqgff	78	22	20	20	CD
cord-271701-tx0lqgff	78	23	C	c	NN
cord-271701-tx0lqgff	78	24	with	with	IN
cord-271701-tx0lqgff	78	25	0.2	0.2	CD
cord-271701-tx0lqgff	78	26	nM	nm	CD
cord-271701-tx0lqgff	78	27	of	of	IN
cord-271701-tx0lqgff	78	28	32	32	CD
cord-271701-tx0lqgff	78	29	P	p	NN
cord-271701-tx0lqgff	78	30	-	-	HYPH
cord-271701-tx0lqgff	78	31	labelled	label	VBN
cord-271701-tx0lqgff	78	32	duplex	duplex	NN
cord-271701-tx0lqgff	78	33	RNA	rna	NN
cord-271701-tx0lqgff	78	34	.	.	.
cord-271701-tx0lqgff	79	1	Subsequently	subsequently	RB
cord-271701-tx0lqgff	79	2	,	,	,
cord-271701-tx0lqgff	79	3	samples	sample	NNS
cord-271701-tx0lqgff	79	4	were	be	VBD
cord-271701-tx0lqgff	79	5	directly	directly	RB
cord-271701-tx0lqgff	79	6	loaded	load	VBN
cord-271701-tx0lqgff	79	7	onto	onto	IN
cord-271701-tx0lqgff	79	8	8	8	CD
cord-271701-tx0lqgff	79	9	%	%	NN
cord-271701-tx0lqgff	79	10	polyacrylamide	polyacrylamide	NN
cord-271701-tx0lqgff	79	11	gels	gel	NNS
cord-271701-tx0lqgff	79	12	containing	contain	VBG
cord-271701-tx0lqgff	79	13	5	5	CD
cord-271701-tx0lqgff	79	14	%	%	NN
cord-271701-tx0lqgff	79	15	glycerol	glycerol	NN
cord-271701-tx0lqgff	79	16	and	and	CC
cord-271701-tx0lqgff	79	17	0.5x	0.5x	CD
cord-271701-tx0lqgff	79	18	TGE	TGE	NNP
cord-271701-tx0lqgff	80	1	(	(	-LRB-
cord-271701-tx0lqgff	80	2	25	25	CD
cord-271701-tx0lqgff	80	3	mM	mM	NNP
cord-271701-tx0lqgff	80	4	Tris	Tris	NNP
cord-271701-tx0lqgff	80	5	,	,	,
cord-271701-tx0lqgff	80	6	190	190	CD
cord-271701-tx0lqgff	80	7	mM	mm	CD
cord-271701-tx0lqgff	80	8	glycine	glycine	NN
cord-271701-tx0lqgff	80	9	and	and	CC
cord-271701-tx0lqgff	80	10	10	10	CD
cord-271701-tx0lqgff	80	11	mM	mm	CD
cord-271701-tx0lqgff	80	12	EDTA	EDTA	NNP
cord-271701-tx0lqgff	80	13	)	)	-RRB-
cord-271701-tx0lqgff	80	14	buffer	buffer	NN
cord-271701-tx0lqgff	80	15	and	and	CC
cord-271701-tx0lqgff	80	16	run	run	VB
cord-271701-tx0lqgff	80	17	at	at	IN
cord-271701-tx0lqgff	80	18	150	150	CD
cord-271701-tx0lqgff	80	19	V	v	NN
cord-271701-tx0lqgff	80	20	for	for	IN
cord-271701-tx0lqgff	80	21	1	1	CD
cord-271701-tx0lqgff	80	22	h	h	NN
cord-271701-tx0lqgff	80	23	at	at	IN
cord-271701-tx0lqgff	80	24	4	4	CD
cord-271701-tx0lqgff	80	25	C.	C.	NNP
cord-271701-tx0lqgff	80	26	Gels	gel	NNS
cord-271701-tx0lqgff	80	27	were	be	VBD
cord-271701-tx0lqgff	80	28	dried	dry	VBN
cord-271701-tx0lqgff	80	29	on	on	IN
cord-271701-tx0lqgff	80	30	Whatman	Whatman	NNP
cord-271701-tx0lqgff	80	31	filter	filter	NN
cord-271701-tx0lqgff	80	32	paper	paper	NN
cord-271701-tx0lqgff	80	33	and	and	CC
cord-271701-tx0lqgff	80	34	bands	band	NNS
cord-271701-tx0lqgff	80	35	were	be	VBD
cord-271701-tx0lqgff	80	36	quantified	quantify	VBN
cord-271701-tx0lqgff	80	37	by	by	IN
cord-271701-tx0lqgff	80	38	phosphorimaging	phosphorimage	VBG
cord-271701-tx0lqgff	80	39	using	use	VBG
cord-271701-tx0lqgff	80	40	a	a	DT
cord-271701-tx0lqgff	80	41	Typhoon	Typhoon	NNP
cord-271701-tx0lqgff	80	42	variable	variable	JJ
cord-271701-tx0lqgff	80	43	mode	mode	NN
cord-271701-tx0lqgff	80	44	scanner	scanner	NN
cord-271701-tx0lqgff	80	45	(	(	-LRB-
cord-271701-tx0lqgff	80	46	GE	GE	NNP
cord-271701-tx0lqgff	80	47	Healthcare	Healthcare	NNP
cord-271701-tx0lqgff	80	48	)	)	-RRB-
cord-271701-tx0lqgff	80	49	and	and	CC
cord-271701-tx0lqgff	80	50	ImageQuant	ImageQuant	NNP
cord-271701-tx0lqgff	80	51	TL	TL	NNP
cord-271701-tx0lqgff	80	52	7.0	7.0	CD
cord-271701-tx0lqgff	80	53	software	software	NN
cord-271701-tx0lqgff	80	54	(	(	-LRB-
cord-271701-tx0lqgff	80	55	GE	GE	NNP
cord-271701-tx0lqgff	80	56	Healthcare	Healthcare	NNP
cord-271701-tx0lqgff	80	57	)	)	-RRB-
cord-271701-tx0lqgff	80	58	as	as	IN
cord-271701-tx0lqgff	80	59	described	describe	VBN
cord-271701-tx0lqgff	80	60	elsewhere	elsewhere	RB
cord-271701-tx0lqgff	80	61	(	(	-LRB-
cord-271701-tx0lqgff	80	62	10	10	CD
cord-271701-tx0lqgff	80	63	)	)	-RRB-
cord-271701-tx0lqgff	80	64	.	.	.
cord-271701-tx0lqgff	81	1	Using	use	VBG
cord-271701-tx0lqgff	81	2	the	the	DT
cord-271701-tx0lqgff	81	3	Matlab	Matlab	NNP
cord-271701-tx0lqgff	81	4	2009a	2009a	CD
cord-271701-tx0lqgff	81	5	Curve	Curve	NNP
cord-271701-tx0lqgff	81	6	Fitting	Fitting	NNP
cord-271701-tx0lqgff	81	7	Toolbox	Toolbox	NNP
cord-271701-tx0lqgff	81	8	,	,	,
cord-271701-tx0lqgff	81	9	the	the	DT
cord-271701-tx0lqgff	81	10	percentage	percentage	NN
cord-271701-tx0lqgff	81	11	of	of	IN
cord-271701-tx0lqgff	81	12	bound	bind	VBN
cord-271701-tx0lqgff	81	13	RNA	RNA	NNP
cord-271701-tx0lqgff	81	14	was	be	VBD
cord-271701-tx0lqgff	81	15	fit	fit	JJ
cord-271701-tx0lqgff	81	16	to	to	IN
cord-271701-tx0lqgff	81	17	the	the	DT
cord-271701-tx0lqgff	81	18	Hill	Hill	NNP
cord-271701-tx0lqgff	81	19	equation	equation	NN
cord-271701-tx0lqgff	81	20	,	,	,
cord-271701-tx0lqgff	81	21	which	which	WDT
cord-271701-tx0lqgff	81	22	is	be	VBZ
cord-271701-tx0lqgff	81	23	defined	define	VBN
cord-271701-tx0lqgff	81	24	as	as	IN
cord-271701-tx0lqgff	81	25	:	:	:
cord-271701-tx0lqgff	81	26	RNA	RNA	NNP
cord-271701-tx0lqgff	81	27	bound	bind	VBD
cord-271701-tx0lqgff	81	28	¼	¼	CD
cord-271701-tx0lqgff	81	29	b	b	NN
cord-271701-tx0lqgff	81	30	Á	á	NN
cord-271701-tx0lqgff	81	31	½nsp8	½nsp8	NNP
cord-271701-tx0lqgff	82	1	n	n	NN
cord-271701-tx0lqgff	82	2	=	=	NN
cord-271701-tx0lqgff	82	3	ðK	ðk	NN
cord-271701-tx0lqgff	82	4	n	n	NN
cord-271701-tx0lqgff	82	5	d	d	NN
cord-271701-tx0lqgff	82	6	+	+	CC
cord-271701-tx0lqgff	82	7	½nsp8	½nsp8	NN
cord-271701-tx0lqgff	82	8	n	n	NN
cord-271701-tx0lqgff	82	9	Þ.	þ.	NN
cord-271701-tx0lqgff	83	1	Here	here	RB
cord-271701-tx0lqgff	83	2	b	b	NN
cord-271701-tx0lqgff	83	3	is	be	VBZ
cord-271701-tx0lqgff	83	4	the	the	DT
cord-271701-tx0lqgff	83	5	upper	upper	JJ
cord-271701-tx0lqgff	83	6	binding	binding	NN
cord-271701-tx0lqgff	83	7	limit	limit	NN
cord-271701-tx0lqgff	83	8	,	,	,
cord-271701-tx0lqgff	83	9	½nsp8	½nsp8	VB
cord-271701-tx0lqgff	83	10	the	the	DT
cord-271701-tx0lqgff	83	11	nsp8	nsp8	NNP
cord-271701-tx0lqgff	83	12	concentration	concentration	NN
cord-271701-tx0lqgff	83	13	,	,	,
cord-271701-tx0lqgff	83	14	n	n	CC
cord-271701-tx0lqgff	83	15	the	the	DT
cord-271701-tx0lqgff	83	16	Hill	Hill	NNP
cord-271701-tx0lqgff	83	17	coefficient	coefficient	NN
cord-271701-tx0lqgff	83	18	and	and	CC
cord-271701-tx0lqgff	83	19	K	k	NN
cord-271701-tx0lqgff	83	20	d	d	XX
cord-271701-tx0lqgff	83	21	the	the	DT
cord-271701-tx0lqgff	83	22	dissociation	dissociation	NN
cord-271701-tx0lqgff	83	23	constant	constant	JJ
cord-271701-tx0lqgff	83	24	.	.	.
cord-271701-tx0lqgff	84	1	The	the	DT
cord-271701-tx0lqgff	84	2	oligoribonucleotide	oligoribonucleotide	JJ
cord-271701-tx0lqgff	84	3	substrates	substrate	NNS
cord-271701-tx0lqgff	84	4	used	use	VBN
cord-271701-tx0lqgff	84	5	for	for	IN
cord-271701-tx0lqgff	84	6	polymerase	polymerase	NN
cord-271701-tx0lqgff	84	7	assays	assay	NNS
cord-271701-tx0lqgff	84	8	are	be	VBP
cord-271701-tx0lqgff	84	9	listed	list	VBN
cord-271701-tx0lqgff	84	10	in	in	IN
cord-271701-tx0lqgff	84	11	Table	Table	NNP
cord-271701-tx0lqgff	84	12	1	1	CD
cord-271701-tx0lqgff	84	13	and	and	CC
cord-271701-tx0lqgff	84	14	were	be	VBD
cord-271701-tx0lqgff	84	15	prepared	prepare	VBN
cord-271701-tx0lqgff	84	16	as	as	IN
cord-271701-tx0lqgff	84	17	described	describe	VBN
cord-271701-tx0lqgff	84	18	previously	previously	RB
cord-271701-tx0lqgff	84	19	(	(	-LRB-
cord-271701-tx0lqgff	84	20	10	10	CD
cord-271701-tx0lqgff	84	21	)	)	-RRB-
cord-271701-tx0lqgff	84	22	.	.	.
cord-271701-tx0lqgff	85	1	Primer	primer	NN
cord-271701-tx0lqgff	85	2	-	-	HYPH
cord-271701-tx0lqgff	85	3	extension	extension	NN
cord-271701-tx0lqgff	85	4	assays	assay	NNS
cord-271701-tx0lqgff	85	5	for	for	IN
cord-271701-tx0lqgff	85	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	85	7	,	,	,
cord-271701-tx0lqgff	85	8	the	the	DT
cord-271701-tx0lqgff	85	9	nsp7	nsp7	JJ
cord-271701-tx0lqgff	85	10	-	-	HYPH
cord-271701-tx0lqgff	85	11	8	8	NNP
cord-271701-tx0lqgff	85	12	polyprotein	polyprotein	NN
cord-271701-tx0lqgff	85	13	,	,	,
cord-271701-tx0lqgff	85	14	and	and	CC
cord-271701-tx0lqgff	85	15	the	the	DT
cord-271701-tx0lqgff	85	16	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	85	17	+	+	SYM
cord-271701-tx0lqgff	85	18	8	8	CD
cord-271701-tx0lqgff	85	19	)	)	-RRB-
cord-271701-tx0lqgff	85	20	complex	complex	NN
cord-271701-tx0lqgff	85	21	were	be	VBD
cord-271701-tx0lqgff	85	22	essentially	essentially	RB
cord-271701-tx0lqgff	85	23	performed	perform	VBN
cord-271701-tx0lqgff	85	24	as	as	IN
cord-271701-tx0lqgff	85	25	described	describe	VBN
cord-271701-tx0lqgff	85	26	previously	previously	RB
cord-271701-tx0lqgff	85	27	for	for	IN
cord-271701-tx0lqgff	85	28	SARS	SARS	NNP
cord-271701-tx0lqgff	85	29	-	-	HYPH
cord-271701-tx0lqgff	85	30	CoV	CoV	NNP
cord-271701-tx0lqgff	85	31	nsp12	nsp12	CD
cord-271701-tx0lqgff	85	32	(	(	-LRB-
cord-271701-tx0lqgff	85	33	10	10	CD
cord-271701-tx0lqgff	85	34	,	,	,
cord-271701-tx0lqgff	85	35	11	11	CD
cord-271701-tx0lqgff	85	36	)	)	-RRB-
cord-271701-tx0lqgff	85	37	.	.	.
cord-271701-tx0lqgff	86	1	In	in	IN
cord-271701-tx0lqgff	86	2	each	each	DT
cord-271701-tx0lqgff	86	3	primer	primer	NN
cord-271701-tx0lqgff	86	4	-	-	HYPH
cord-271701-tx0lqgff	86	5	extension	extension	NN
cord-271701-tx0lqgff	86	6	reaction	reaction	NN
cord-271701-tx0lqgff	86	7	,	,	,
cord-271701-tx0lqgff	86	8	typically	typically	RB
cord-271701-tx0lqgff	86	9	1	1	CD
cord-271701-tx0lqgff	86	10	mM	mm	CD
cord-271701-tx0lqgff	86	11	wild	wild	JJ
cord-271701-tx0lqgff	86	12	-	-	HYPH
cord-271701-tx0lqgff	86	13	type	type	NN
cord-271701-tx0lqgff	86	14	or	or	CC
cord-271701-tx0lqgff	86	15	mutant	mutant	JJ
cord-271701-tx0lqgff	86	16	nsp8	nsp8	NN
cord-271701-tx0lqgff	86	17	was	be	VBD
cord-271701-tx0lqgff	86	18	incubated	incubate	VBN
cord-271701-tx0lqgff	86	19	with	with	IN
cord-271701-tx0lqgff	86	20	4	4	CD
cord-271701-tx0lqgff	86	21	mM	mm	CD
cord-271701-tx0lqgff	86	22	MgCl	MgCl	NNP
cord-271701-tx0lqgff	86	23	2	2	CD
cord-271701-tx0lqgff	86	24	,	,	,
cord-271701-tx0lqgff	86	25	50	50	CD
cord-271701-tx0lqgff	86	26	mM	mm	CD
cord-271701-tx0lqgff	86	27	GTP	GTP	NNP
cord-271701-tx0lqgff	86	28	,	,	,
cord-271701-tx0lqgff	86	29	50	50	CD
cord-271701-tx0lqgff	86	30	mM	mm	CD
cord-271701-tx0lqgff	86	31	ATP	ATP	NNP
cord-271701-tx0lqgff	86	32	,	,	,
cord-271701-tx0lqgff	86	33	0.17	0.17	CD
cord-271701-tx0lqgff	86	34	mM	mm	CD
cord-271701-tx0lqgff	86	35	[	[	-LRB-
cord-271701-tx0lqgff	86	36	a-32	a-32	NNP
cord-271701-tx0lqgff	86	37	P]ATP	P]ATP	NNP
cord-271701-tx0lqgff	86	38	,	,	,
cord-271701-tx0lqgff	86	39	1	1	CD
cord-271701-tx0lqgff	86	40	mM	mm	CD
cord-271701-tx0lqgff	86	41	DTT	DTT	NNP
cord-271701-tx0lqgff	86	42	,	,	,
cord-271701-tx0lqgff	86	43	0.1	0.1	CD
cord-271701-tx0lqgff	86	44	%	%	NN
cord-271701-tx0lqgff	86	45	Triton	Triton	NNP
cord-271701-tx0lqgff	86	46	X-100	x-100	NN
cord-271701-tx0lqgff	86	47	,	,	,
cord-271701-tx0lqgff	86	48	10	10	CD
cord-271701-tx0lqgff	86	49	mM	mm	CD
cord-271701-tx0lqgff	86	50	KCl	KCl	NNS
cord-271701-tx0lqgff	86	51	and	and	CC
cord-271701-tx0lqgff	86	52	20	20	CD
cord-271701-tx0lqgff	86	53	mM	mM	NNP
cord-271701-tx0lqgff	86	54	Tris	Tris	NNP
cord-271701-tx0lqgff	86	55	(	(	-LRB-
cord-271701-tx0lqgff	86	56	pH	pH	NNP
cord-271701-tx0lqgff	86	57	9.5	9.5	CD
cord-271701-tx0lqgff	86	58	)	)	-RRB-
cord-271701-tx0lqgff	86	59	.	.	.
cord-271701-tx0lqgff	87	1	At	at	IN
cord-271701-tx0lqgff	87	2	most	most	JJS
cord-271701-tx0lqgff	87	3	,	,	,
cord-271701-tx0lqgff	87	4	10	10	CD
cord-271701-tx0lqgff	87	5	mM	mm	CD
cord-271701-tx0lqgff	87	6	NaCl	nacl	NN
cord-271701-tx0lqgff	87	7	and	and	CC
cord-271701-tx0lqgff	87	8	5	5	CD
cord-271701-tx0lqgff	87	9	%	%	NN
cord-271701-tx0lqgff	87	10	glycerol	glycerol	NN
cord-271701-tx0lqgff	87	11	were	be	VBD
cord-271701-tx0lqgff	87	12	introduced	introduce	VBN
cord-271701-tx0lqgff	87	13	with	with	IN
cord-271701-tx0lqgff	87	14	the	the	DT
cord-271701-tx0lqgff	87	15	nsp8	nsp8	JJ
cord-271701-tx0lqgff	87	16	storage	storage	NN
cord-271701-tx0lqgff	87	17	buffer	buffer	NN
cord-271701-tx0lqgff	87	18	.	.	.
cord-271701-tx0lqgff	88	1	Gels	gel	NNS
cord-271701-tx0lqgff	88	2	were	be	VBD
cord-271701-tx0lqgff	88	3	run	run	VBN
cord-271701-tx0lqgff	88	4	and	and	CC
cord-271701-tx0lqgff	88	5	analysed	analyse	VBN
cord-271701-tx0lqgff	88	6	as	as	IN
cord-271701-tx0lqgff	88	7	described	describe	VBN
cord-271701-tx0lqgff	88	8	previously	previously	RB
cord-271701-tx0lqgff	88	9	(	(	-LRB-
cord-271701-tx0lqgff	88	10	10	10	CD
cord-271701-tx0lqgff	88	11	)	)	-RRB-
cord-271701-tx0lqgff	88	12	.	.	.
cord-271701-tx0lqgff	89	1	To	to	TO
cord-271701-tx0lqgff	89	2	convert	convert	VB
cord-271701-tx0lqgff	89	3	the	the	DT
cord-271701-tx0lqgff	89	4	phosphorimager	phosphorimager	NN
cord-271701-tx0lqgff	89	5	signal	signal	NN
cord-271701-tx0lqgff	89	6	into	into	IN
cord-271701-tx0lqgff	89	7	the	the	DT
cord-271701-tx0lqgff	89	8	amount	amount	NN
cord-271701-tx0lqgff	89	9	of	of	IN
cord-271701-tx0lqgff	89	10	[	[	-LRB-
cord-271701-tx0lqgff	89	11	a-32	a-32	NNP
cord-271701-tx0lqgff	89	12	P]AMP	P]AMP	NNP
cord-271701-tx0lqgff	89	13	incorporated	incorporate	VBD
cord-271701-tx0lqgff	89	14	,	,	,
cord-271701-tx0lqgff	89	15	a	a	DT
cord-271701-tx0lqgff	89	16	10	10	CD
cord-271701-tx0lqgff	89	17	À2	à2	NN
cord-271701-tx0lqgff	89	18	to	to	IN
cord-271701-tx0lqgff	89	19	10	10	CD
cord-271701-tx0lqgff	89	20	À5	À5	NNP
cord-271701-tx0lqgff	89	21	dilution	dilution	NN
cord-271701-tx0lqgff	89	22	series	series	NN
cord-271701-tx0lqgff	89	23	of	of	IN
cord-271701-tx0lqgff	89	24	the	the	DT
cord-271701-tx0lqgff	89	25	[	[	-LRB-
cord-271701-tx0lqgff	89	26	a-32	a-32	NNP
cord-271701-tx0lqgff	89	27	P]ATP	P]ATP	NNP
cord-271701-tx0lqgff	89	28	stock	stock	NN
cord-271701-tx0lqgff	89	29	was	be	VBD
cord-271701-tx0lqgff	89	30	spotted	spot	VBN
cord-271701-tx0lqgff	89	31	in	in	IN
cord-271701-tx0lqgff	89	32	triplicate	triplicate	NN
cord-271701-tx0lqgff	89	33	on	on	IN
cord-271701-tx0lqgff	89	34	Whatman	Whatman	NNP
cord-271701-tx0lqgff	89	35	filter	filter	NN
cord-271701-tx0lqgff	89	36	paper	paper	NN
cord-271701-tx0lqgff	89	37	and	and	CC
cord-271701-tx0lqgff	89	38	exposed	expose	VBN
cord-271701-tx0lqgff	89	39	alongside	alongside	IN
cord-271701-tx0lqgff	89	40	the	the	DT
cord-271701-tx0lqgff	89	41	PAGE	page	NN
cord-271701-tx0lqgff	89	42	gel	gel	NN
cord-271701-tx0lqgff	89	43	.	.	.
cord-271701-tx0lqgff	90	1	The	the	DT
cord-271701-tx0lqgff	90	2	amount	amount	NN
cord-271701-tx0lqgff	90	3	of	of	IN
cord-271701-tx0lqgff	90	4	incorporated	incorporated	JJ
cord-271701-tx0lqgff	90	5	label	label	NN
cord-271701-tx0lqgff	90	6	was	be	VBD
cord-271701-tx0lqgff	90	7	ultimately	ultimately	RB
cord-271701-tx0lqgff	90	8	corrected	correct	VBN
cord-271701-tx0lqgff	90	9	for	for	IN
cord-271701-tx0lqgff	90	10	the	the	DT
cord-271701-tx0lqgff	90	11	concentration	concentration	NN
cord-271701-tx0lqgff	90	12	of	of	IN
cord-271701-tx0lqgff	90	13	competing	compete	VBG
cord-271701-tx0lqgff	90	14	,	,	,
cord-271701-tx0lqgff	90	15	unlabelled	unlabelled	JJ
cord-271701-tx0lqgff	90	16	nucleotides	nucleotide	NNS
cord-271701-tx0lqgff	90	17	present	present	JJ
cord-271701-tx0lqgff	90	18	in	in	IN
cord-271701-tx0lqgff	90	19	the	the	DT
cord-271701-tx0lqgff	90	20	reaction	reaction	NN
cord-271701-tx0lqgff	90	21	mixture	mixture	NN
cord-271701-tx0lqgff	90	22	.	.	.
cord-271701-tx0lqgff	91	1	De	De	NNP
cord-271701-tx0lqgff	91	2	novo	novo	NNP
cord-271701-tx0lqgff	91	3	initiation	initiation	NN
cord-271701-tx0lqgff	91	4	assays	assay	NNS
cord-271701-tx0lqgff	91	5	were	be	VBD
cord-271701-tx0lqgff	91	6	essentially	essentially	RB
cord-271701-tx0lqgff	91	7	performed	perform	VBN
cord-271701-tx0lqgff	91	8	as	as	IN
cord-271701-tx0lqgff	91	9	described	describe	VBN
cord-271701-tx0lqgff	91	10	by	by	IN
cord-271701-tx0lqgff	91	11	Imbert	Imbert	NNP
cord-271701-tx0lqgff	91	12	et	et	NNP
cord-271701-tx0lqgff	91	13	al	al	NNP
cord-271701-tx0lqgff	91	14	.	.	.
cord-271701-tx0lqgff	92	1	(	(	-LRB-
cord-271701-tx0lqgff	92	2	12	12	CD
cord-271701-tx0lqgff	92	3	)	)	-RRB-
cord-271701-tx0lqgff	92	4	,	,	,
cord-271701-tx0lqgff	92	5	with	with	IN
cord-271701-tx0lqgff	92	6	small	small	JJ
cord-271701-tx0lqgff	92	7	modifications	modification	NNS
cord-271701-tx0lqgff	92	8	for	for	IN
cord-271701-tx0lqgff	92	9	optimisation	optimisation	NN
cord-271701-tx0lqgff	92	10	.	.	.
cord-271701-tx0lqgff	93	1	Briefly	briefly	RB
cord-271701-tx0lqgff	93	2	,	,	,
cord-271701-tx0lqgff	93	3	1	1	CD
cord-271701-tx0lqgff	93	4	mM	mm	CD
cord-271701-tx0lqgff	93	5	wild	wild	JJ
cord-271701-tx0lqgff	93	6	-	-	HYPH
cord-271701-tx0lqgff	93	7	type	type	NN
cord-271701-tx0lqgff	93	8	or	or	CC
cord-271701-tx0lqgff	93	9	mutant	mutant	JJ
cord-271701-tx0lqgff	93	10	nsp8	nsp8	NN
cord-271701-tx0lqgff	93	11	was	be	VBD
cord-271701-tx0lqgff	93	12	incubated	incubate	VBN
cord-271701-tx0lqgff	93	13	with	with	IN
cord-271701-tx0lqgff	93	14	4	4	CD
cord-271701-tx0lqgff	93	15	mM	mm	CD
cord-271701-tx0lqgff	93	16	MgCl	MgCl	NNP
cord-271701-tx0lqgff	93	17	2	2	CD
cord-271701-tx0lqgff	93	18	,	,	,
cord-271701-tx0lqgff	93	19	1	1	CD
cord-271701-tx0lqgff	93	20	mM	mm	CD
cord-271701-tx0lqgff	93	21	MnCl	MnCl	NNP
cord-271701-tx0lqgff	93	22	2	2	CD
cord-271701-tx0lqgff	93	23	,	,	,
cord-271701-tx0lqgff	94	1	1	1	LS
cord-271701-tx0lqgff	94	2	mM	mm	CD
cord-271701-tx0lqgff	94	3	GTP	GTP	NNP
cord-271701-tx0lqgff	94	4	,	,	,
cord-271701-tx0lqgff	94	5	5	5	CD
cord-271701-tx0lqgff	94	6	mM	mm	CD
cord-271701-tx0lqgff	94	7	ATP	ATP	NNP
cord-271701-tx0lqgff	94	8	,	,	,
cord-271701-tx0lqgff	94	9	0.17	0.17	CD
cord-271701-tx0lqgff	94	10	mM	mm	CD
cord-271701-tx0lqgff	94	11	[	[	-LRB-
cord-271701-tx0lqgff	94	12	a-32	a-32	NNP
cord-271701-tx0lqgff	94	13	P]ATP	P]ATP	NNP
cord-271701-tx0lqgff	94	14	and	and	CC
cord-271701-tx0lqgff	94	15	1	1	CD
cord-271701-tx0lqgff	94	16	mM	mm	CD
cord-271701-tx0lqgff	94	17	of	of	IN
cord-271701-tx0lqgff	94	18	oligo	oligo	NN
cord-271701-tx0lqgff	94	19	AFMB131	AFMB131	NNP
cord-271701-tx0lqgff	94	20	.	.	.
cord-271701-tx0lqgff	95	1	Alignments	alignment	NNS
cord-271701-tx0lqgff	95	2	of	of	IN
cord-271701-tx0lqgff	95	3	nsp8	nsp8	JJ
cord-271701-tx0lqgff	95	4	sequences	sequence	NNS
cord-271701-tx0lqgff	95	5	were	be	VBD
cord-271701-tx0lqgff	95	6	made	make	VBN
cord-271701-tx0lqgff	95	7	using	use	VBG
cord-271701-tx0lqgff	95	8	Muscle	Muscle	NNP
cord-271701-tx0lqgff	95	9	(	(	-LRB-
cord-271701-tx0lqgff	95	10	16	16	CD
cord-271701-tx0lqgff	95	11	)	)	-RRB-
cord-271701-tx0lqgff	95	12	.	.	.
cord-271701-tx0lqgff	96	1	Sequences	sequence	NNS
cord-271701-tx0lqgff	96	2	used	use	VBD
cord-271701-tx0lqgff	96	3	included	include	VBD
cord-271701-tx0lqgff	96	4	the	the	DT
cord-271701-tx0lqgff	96	5	alphacoronaviruses	alphacoronaviruses	NNP
cord-271701-tx0lqgff	96	6	human	human	NNP
cord-271701-tx0lqgff	96	7	CoV	CoV	NNP
cord-271701-tx0lqgff	96	8	229E	229e	CD
cord-271701-tx0lqgff	97	1	(	(	-LRB-
cord-271701-tx0lqgff	97	2	NC_002645	NC_002645	NNP
cord-271701-tx0lqgff	97	3	)	)	-RRB-
cord-271701-tx0lqgff	97	4	,	,	,
cord-271701-tx0lqgff	97	5	human	human	JJ
cord-271701-tx0lqgff	97	6	CoV	cov	NN
cord-271701-tx0lqgff	98	1	NL63	NL63	NNP
cord-271701-tx0lqgff	98	2	(	(	-LRB-
cord-271701-tx0lqgff	98	3	NC_005831	NC_005831	NNP
cord-271701-tx0lqgff	98	4	)	)	-RRB-
cord-271701-tx0lqgff	98	5	,	,	,
cord-271701-tx0lqgff	98	6	and	and	CC
cord-271701-tx0lqgff	98	7	bat	bat	NN
cord-271701-tx0lqgff	98	8	CoV	cov	NN
cord-271701-tx0lqgff	98	9	HKU8	HKU8	NNP
cord-271701-tx0lqgff	98	10	(	(	-LRB-
cord-271701-tx0lqgff	98	11	NC_010438	NC_010438	NNP
cord-271701-tx0lqgff	98	12	)	)	-RRB-
cord-271701-tx0lqgff	98	13	;	;	:
cord-271701-tx0lqgff	98	14	the	the	DT
cord-271701-tx0lqgff	98	15	betacoronaviruses	betacoronaviruses	NNP
cord-271701-tx0lqgff	98	16	SARS	SARS	NNP
cord-271701-tx0lqgff	98	17	-	-	HYPH
cord-271701-tx0lqgff	98	18	CoV	CoV	NNP
cord-271701-tx0lqgff	98	19	Frankfurt-1	Frankfurt-1	NNP
cord-271701-tx0lqgff	98	20	(	(	-LRB-
cord-271701-tx0lqgff	98	21	AY291315	AY291315	NNP
cord-271701-tx0lqgff	98	22	)	)	-RRB-
cord-271701-tx0lqgff	98	23	,	,	,
cord-271701-tx0lqgff	98	24	mouse	mouse	NN
cord-271701-tx0lqgff	98	25	hepatitis	hepatitis	NN
cord-271701-tx0lqgff	98	26	virus	virus	NN
cord-271701-tx0lqgff	98	27	A59	A59	NNP
cord-271701-tx0lqgff	98	28	(	(	-LRB-
cord-271701-tx0lqgff	98	29	MHV	MHV	NNP
cord-271701-tx0lqgff	98	30	,	,	,
cord-271701-tx0lqgff	98	31	NC_001849	NC_001849	NNP
cord-271701-tx0lqgff	98	32	)	)	-RRB-
cord-271701-tx0lqgff	98	33	and	and	CC
cord-271701-tx0lqgff	98	34	human	human	JJ
cord-271701-tx0lqgff	98	35	CoV	cov	NN
cord-271701-tx0lqgff	98	36	OC43	OC43	NNP
cord-271701-tx0lqgff	98	37	(	(	-LRB-
cord-271701-tx0lqgff	98	38	NC_005147	NC_005147	NNP
cord-271701-tx0lqgff	98	39	)	)	-RRB-
cord-271701-tx0lqgff	98	40	;	;	:
cord-271701-tx0lqgff	98	41	and	and	CC
cord-271701-tx0lqgff	98	42	the	the	DT
cord-271701-tx0lqgff	98	43	gammacoronaviruses	gammacoronaviruses	NNP
cord-271701-tx0lqgff	98	44	beluga	beluga	NNP
cord-271701-tx0lqgff	98	45	whale	whale	NNP
cord-271701-tx0lqgff	98	46	CoV	CoV	NNP
cord-271701-tx0lqgff	98	47	SW1	SW1	NNP
cord-271701-tx0lqgff	98	48	(	(	-LRB-
cord-271701-tx0lqgff	98	49	NC010646	NC010646	NNP
cord-271701-tx0lqgff	98	50	)	)	-RRB-
cord-271701-tx0lqgff	98	51	,	,	,
cord-271701-tx0lqgff	98	52	turkey	turkey	NNP
cord-271701-tx0lqgff	98	53	CoV	CoV	NNP
cord-271701-tx0lqgff	98	54	(	(	-LRB-
cord-271701-tx0lqgff	98	55	NC_010800	NC_010800	NNP
cord-271701-tx0lqgff	98	56	)	)	-RRB-
cord-271701-tx0lqgff	98	57	and	and	CC
cord-271701-tx0lqgff	98	58	avian	avian	JJ
cord-271701-tx0lqgff	98	59	infectious	infectious	JJ
cord-271701-tx0lqgff	98	60	bronchitis	bronchitis	NN
cord-271701-tx0lqgff	98	61	virus	virus	NN
cord-271701-tx0lqgff	98	62	(	(	-LRB-
cord-271701-tx0lqgff	98	63	IBV	IBV	NNP
cord-271701-tx0lqgff	98	64	,	,	,
cord-271701-tx0lqgff	98	65	AJ311317	AJ311317	NNP
cord-271701-tx0lqgff	98	66	)	)	-RRB-
cord-271701-tx0lqgff	98	67	.	.	.
cord-271701-tx0lqgff	99	1	N	n	CD
cord-271701-tx0lqgff	99	2	-	-	HYPH
cord-271701-tx0lqgff	99	3	terminal	terminal	NN
cord-271701-tx0lqgff	99	4	processing	processing	NN
cord-271701-tx0lqgff	99	5	defines	define	VBZ
cord-271701-tx0lqgff	99	6	nsp8	nsp8	JJ
cord-271701-tx0lqgff	99	7	multimerization	multimerization	NN
cord-271701-tx0lqgff	99	8	and	and	CC
cord-271701-tx0lqgff	99	9	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	99	10	+	+	SYM
cord-271701-tx0lqgff	99	11	8	8	CD
cord-271701-tx0lqgff	99	12	)	)	-RRB-
cord-271701-tx0lqgff	100	1	complex	complex	JJ
cord-271701-tx0lqgff	100	2	formation	formation	NN
cord-271701-tx0lqgff	100	3	SARS	SARS	NNP
cord-271701-tx0lqgff	100	4	-	-	HYPH
cord-271701-tx0lqgff	100	5	CoV	CoV	NNP
cord-271701-tx0lqgff	100	6	nsp7	nsp7	NN
cord-271701-tx0lqgff	100	7	and	and	CC
cord-271701-tx0lqgff	100	8	nsp8	nsp8	NN
cord-271701-tx0lqgff	100	9	were	be	VBD
cord-271701-tx0lqgff	100	10	previously	previously	RB
cord-271701-tx0lqgff	100	11	reported	report	VBN
cord-271701-tx0lqgff	100	12	to	to	TO
cord-271701-tx0lqgff	100	13	interact	interact	VB
cord-271701-tx0lqgff	100	14	and	and	CC
cord-271701-tx0lqgff	100	15	form	form	VB
cord-271701-tx0lqgff	100	16	a	a	DT
cord-271701-tx0lqgff	100	17	hollow	hollow	JJ
cord-271701-tx0lqgff	100	18	ring	ring	NN
cord-271701-tx0lqgff	100	19	structure	structure	NN
cord-271701-tx0lqgff	100	20	that	that	WDT
cord-271701-tx0lqgff	100	21	is	be	VBZ
cord-271701-tx0lqgff	100	22	composed	compose	VBN
cord-271701-tx0lqgff	100	23	of	of	IN
cord-271701-tx0lqgff	100	24	an	an	DT
cord-271701-tx0lqgff	100	25	intricate	intricate	JJ
cord-271701-tx0lqgff	100	26	nsp8	nsp8	JJ
cord-271701-tx0lqgff	100	27	octamer	octamer	NN
cord-271701-tx0lqgff	100	28	supported	support	VBN
cord-271701-tx0lqgff	100	29	by	by	IN
cord-271701-tx0lqgff	100	30	 	 	_SP
cord-271701-tx0lqgff	100	31	eight	eight	CD
cord-271701-tx0lqgff	100	32	copies	copy	NNS
cord-271701-tx0lqgff	100	33	of	of	IN
cord-271701-tx0lqgff	100	34	nsp7	nsp7	JJ
cord-271701-tx0lqgff	100	35	(	(	-LRB-
cord-271701-tx0lqgff	100	36	13,17	13,17	CD
cord-271701-tx0lqgff	100	37	)	)	-RRB-
cord-271701-tx0lqgff	101	1	(	(	-LRB-
cord-271701-tx0lqgff	101	2	Figure	figure	NN
cord-271701-tx0lqgff	101	3	1B	1b	CD
cord-271701-tx0lqgff	101	4	)	)	-RRB-
cord-271701-tx0lqgff	101	5	.	.	.
cord-271701-tx0lqgff	102	1	Based	base	VBN
cord-271701-tx0lqgff	102	2	on	on	IN
cord-271701-tx0lqgff	102	3	the	the	DT
cord-271701-tx0lqgff	102	4	large	large	JJ
cord-271701-tx0lqgff	102	5	diameter	diameter	NN
cord-271701-tx0lqgff	102	6	,	,	,
cord-271701-tx0lqgff	102	7	positive	positive	JJ
cord-271701-tx0lqgff	102	8	charge	charge	NN
cord-271701-tx0lqgff	102	9	of	of	IN
cord-271701-tx0lqgff	102	10	the	the	DT
cord-271701-tx0lqgff	102	11	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	102	12	's	's	POS
cord-271701-tx0lqgff	102	13	channel	channel	NN
cord-271701-tx0lqgff	102	14	and	and	CC
cord-271701-tx0lqgff	102	15	in	in	IN
cord-271701-tx0lqgff	102	16	silico	silico	NN
cord-271701-tx0lqgff	102	17	docking	docking	NN
cord-271701-tx0lqgff	102	18	,	,	,
cord-271701-tx0lqgff	102	19	it	-PRON-	PRP
cord-271701-tx0lqgff	102	20	was	be	VBD
cord-271701-tx0lqgff	102	21	proposed	propose	VBN
cord-271701-tx0lqgff	102	22	to	to	TO
cord-271701-tx0lqgff	102	23	be	be	VB
cord-271701-tx0lqgff	102	24	able	able	JJ
cord-271701-tx0lqgff	102	25	to	to	TO
cord-271701-tx0lqgff	102	26	encircle	encircle	VB
cord-271701-tx0lqgff	102	27	dsRNA	dsrna	JJ
cord-271701-tx0lqgff	103	1	(	(	-LRB-
cord-271701-tx0lqgff	103	2	Figure	figure	NN
cord-271701-tx0lqgff	103	3	1B	1b	CD
cord-271701-tx0lqgff	103	4	)	)	-RRB-
cord-271701-tx0lqgff	103	5	.	.	.
cord-271701-tx0lqgff	104	1	However	however	RB
cord-271701-tx0lqgff	104	2	,	,	,
cord-271701-tx0lqgff	104	3	the	the	DT
cord-271701-tx0lqgff	104	4	functional	functional	JJ
cord-271701-tx0lqgff	104	5	significance	significance	NN
cord-271701-tx0lqgff	104	6	of	of	IN
cord-271701-tx0lqgff	104	7	the	the	DT
cord-271701-tx0lqgff	104	8	compound	compound	NN
cord-271701-tx0lqgff	104	9	interactions	interaction	NNS
cord-271701-tx0lqgff	104	10	between	between	IN
cord-271701-tx0lqgff	104	11	nsp7	nsp7	NN
cord-271701-tx0lqgff	104	12	and	and	CC
cord-271701-tx0lqgff	104	13	nsp8	nsp8	NN
cord-271701-tx0lqgff	104	14	is	be	VBZ
cord-271701-tx0lqgff	104	15	poorly	poorly	RB
cord-271701-tx0lqgff	104	16	understood	understand	VBN
cord-271701-tx0lqgff	104	17	,	,	,
cord-271701-tx0lqgff	104	18	as	as	IN
cord-271701-tx0lqgff	104	19	are	be	VBP
cord-271701-tx0lqgff	104	20	the	the	DT
cord-271701-tx0lqgff	104	21	polymerase	polymerase	NN
cord-271701-tx0lqgff	104	22	activities	activity	NNS
cord-271701-tx0lqgff	104	23	associated	associate	VBN
cord-271701-tx0lqgff	104	24	with	with	IN
cord-271701-tx0lqgff	104	25	monomeric	monomeric	JJ
cord-271701-tx0lqgff	104	26	nsp8	nsp8	NN
cord-271701-tx0lqgff	104	27	or	or	CC
cord-271701-tx0lqgff	104	28	nsp8-containing	nsp8-containing	NNP
cord-271701-tx0lqgff	104	29	multimers	multimer	NNS
cord-271701-tx0lqgff	104	30	.	.	.
cord-271701-tx0lqgff	105	1	So	so	RB
cord-271701-tx0lqgff	105	2	far	far	RB
cord-271701-tx0lqgff	105	3	,	,	,
cord-271701-tx0lqgff	105	4	strategies	strategy	NNS
cord-271701-tx0lqgff	105	5	for	for	IN
cord-271701-tx0lqgff	105	6	the	the	DT
cord-271701-tx0lqgff	105	7	purification	purification	NN
cord-271701-tx0lqgff	105	8	of	of	IN
cord-271701-tx0lqgff	105	9	recombinant	recombinant	JJ
cord-271701-tx0lqgff	105	10	nsp8	nsp8	NNP
cord-271701-tx0lqgff	105	11	have	have	VBP
cord-271701-tx0lqgff	105	12	involved	involve	VBN
cord-271701-tx0lqgff	105	13	the	the	DT
cord-271701-tx0lqgff	105	14	use	use	NN
cord-271701-tx0lqgff	105	15	of	of	IN
cord-271701-tx0lqgff	105	16	affinity	affinity	NN
cord-271701-tx0lqgff	105	17	tags	tag	NNS
cord-271701-tx0lqgff	105	18	[	[	-LRB-
cord-271701-tx0lqgff	105	19	e.g.	e.g.	PDT
cord-271701-tx0lqgff	105	20	His	-PRON-	PRP$
cord-271701-tx0lqgff	105	21	6	6	CD
cord-271701-tx0lqgff	105	22	or	or	CC
cord-271701-tx0lqgff	105	23	glutathione	glutathione	NN
cord-271701-tx0lqgff	105	24	-	-	HYPH
cord-271701-tx0lqgff	105	25	S	s	NN
cord-271701-tx0lqgff	105	26	-	-	HYPH
cord-271701-tx0lqgff	105	27	transferase	transferase	NN
cord-271701-tx0lqgff	105	28	(	(	-LRB-
cord-271701-tx0lqgff	105	29	GST	GST	NNP
cord-271701-tx0lqgff	105	30	)	)	-RRB-
cord-271701-tx0lqgff	105	31	(	(	-LRB-
cord-271701-tx0lqgff	105	32	12	12	CD
cord-271701-tx0lqgff	105	33	,	,	,
cord-271701-tx0lqgff	105	34	13	13	CD
cord-271701-tx0lqgff	105	35	)	)	-RRB-
cord-271701-tx0lqgff	105	36	]	]	-RRB-
cord-271701-tx0lqgff	105	37	that	that	WDT
cord-271701-tx0lqgff	105	38	were	be	VBD
cord-271701-tx0lqgff	105	39	fused	fuse	VBN
cord-271701-tx0lqgff	105	40	to	to	IN
cord-271701-tx0lqgff	105	41	one	one	CD
cord-271701-tx0lqgff	105	42	terminus	terminus	NN
cord-271701-tx0lqgff	105	43	to	to	TO
cord-271701-tx0lqgff	105	44	facilitate	facilitate	VB
cord-271701-tx0lqgff	105	45	protein	protein	NN
cord-271701-tx0lqgff	105	46	recovery	recovery	NN
cord-271701-tx0lqgff	105	47	.	.	.
cord-271701-tx0lqgff	106	1	Inadvertently	inadvertently	RB
cord-271701-tx0lqgff	106	2	though	though	RB
cord-271701-tx0lqgff	106	3	,	,	,
cord-271701-tx0lqgff	106	4	such	such	JJ
cord-271701-tx0lqgff	106	5	tags	tag	NNS
cord-271701-tx0lqgff	106	6	or	or	CC
cord-271701-tx0lqgff	106	7	other	other	JJ
cord-271701-tx0lqgff	106	8	exogenous	exogenous	JJ
cord-271701-tx0lqgff	106	9	sequences	sequence	NNS
cord-271701-tx0lqgff	106	10	may	may	MD
cord-271701-tx0lqgff	106	11	significantly	significantly	RB
cord-271701-tx0lqgff	106	12	impede	impede	VB
cord-271701-tx0lqgff	106	13	the	the	DT
cord-271701-tx0lqgff	106	14	correct	correct	JJ
cord-271701-tx0lqgff	106	15	folding	folding	NN
cord-271701-tx0lqgff	106	16	of	of	IN
cord-271701-tx0lqgff	106	17	enzymes	enzyme	NNS
cord-271701-tx0lqgff	106	18	and	and	CC
cord-271701-tx0lqgff	106	19	thus	thus	RB
cord-271701-tx0lqgff	106	20	alter	alter	VB
cord-271701-tx0lqgff	106	21	their	-PRON-	PRP$
cord-271701-tx0lqgff	106	22	stability	stability	NN
cord-271701-tx0lqgff	106	23	or	or	CC
cord-271701-tx0lqgff	106	24	activity	activity	NN
cord-271701-tx0lqgff	106	25	,	,	,
cord-271701-tx0lqgff	106	26	as	as	IN
cord-271701-tx0lqgff	106	27	exemplified	exemplify	VBN
cord-271701-tx0lqgff	106	28	by	by	IN
cord-271701-tx0lqgff	106	29	studies	study	NNS
cord-271701-tx0lqgff	106	30	of	of	IN
cord-271701-tx0lqgff	106	31	the	the	DT
cord-271701-tx0lqgff	106	32	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	106	33	(	(	-LRB-
cord-271701-tx0lqgff	106	34	3D	3D	NNP
cord-271701-tx0lqgff	106	35	pol	pol	NNP
cord-271701-tx0lqgff	106	36	)	)	-RRB-
cord-271701-tx0lqgff	106	37	and	and	CC
cord-271701-tx0lqgff	106	38	SARS	SARS	NNP
cord-271701-tx0lqgff	106	39	-	-	HYPH
cord-271701-tx0lqgff	106	40	CoV	CoV	NNP
cord-271701-tx0lqgff	106	41	(	(	-LRB-
cord-271701-tx0lqgff	106	42	nsp12	nsp12	CD
cord-271701-tx0lqgff	106	43	)	)	-RRB-
cord-271701-tx0lqgff	106	44	RdRp	RdRp	NNP
cord-271701-tx0lqgff	106	45	subunits	subunit	NNS
cord-271701-tx0lqgff	106	46	(	(	-LRB-
cord-271701-tx0lqgff	106	47	10	10	CD
cord-271701-tx0lqgff	106	48	,	,	,
cord-271701-tx0lqgff	106	49	14	14	CD
cord-271701-tx0lqgff	106	50	,	,	,
cord-271701-tx0lqgff	106	51	18	18	CD
cord-271701-tx0lqgff	106	52	)	)	-RRB-
cord-271701-tx0lqgff	106	53	.	.	.
cord-271701-tx0lqgff	107	1	To	to	TO
cord-271701-tx0lqgff	107	2	circumvent	circumvent	VB
cord-271701-tx0lqgff	107	3	this	this	DT
cord-271701-tx0lqgff	107	4	issue	issue	NN
cord-271701-tx0lqgff	107	5	,	,	,
cord-271701-tx0lqgff	107	6	we	-PRON-	PRP
cord-271701-tx0lqgff	107	7	developed	develop	VBD
cord-271701-tx0lqgff	107	8	a	a	DT
cord-271701-tx0lqgff	107	9	protocol	protocol	NN
cord-271701-tx0lqgff	107	10	in	in	IN
cord-271701-tx0lqgff	107	11	which	which	WDT
cord-271701-tx0lqgff	107	12	SARS	SARS	NNP
cord-271701-tx0lqgff	107	13	-	-	HYPH
cord-271701-tx0lqgff	107	14	CoV	CoV	NNP
cord-271701-tx0lqgff	107	15	nsp8	nsp8	NN
cord-271701-tx0lqgff	107	16	was	be	VBD
cord-271701-tx0lqgff	107	17	expressed	express	VBN
cord-271701-tx0lqgff	107	18	as	as	IN
cord-271701-tx0lqgff	107	19	a	a	DT
cord-271701-tx0lqgff	107	20	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	107	21	(	(	-LRB-
cord-271701-tx0lqgff	107	22	ub	ub	NNP
cord-271701-tx0lqgff	107	23	)	)	-RRB-
cord-271701-tx0lqgff	107	24	fusion	fusion	NN
cord-271701-tx0lqgff	107	25	protein	protein	NN
cord-271701-tx0lqgff	107	26	carrying	carry	VBG
cord-271701-tx0lqgff	107	27	a	a	DT
cord-271701-tx0lqgff	107	28	C	c	NN
cord-271701-tx0lqgff	107	29	-	-	HYPH
cord-271701-tx0lqgff	107	30	terminal	terminal	NN
cord-271701-tx0lqgff	108	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	108	2	6	6	CD
cord-271701-tx0lqgff	108	3	-tag	-tag	:
cord-271701-tx0lqgff	108	4	(	(	-LRB-
cord-271701-tx0lqgff	108	5	ub	ub	NNP
cord-271701-tx0lqgff	108	6	-	-	HYPH
cord-271701-tx0lqgff	108	7	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	108	8	)	)	-RRB-
cord-271701-tx0lqgff	108	9	,	,	,
cord-271701-tx0lqgff	108	10	which	which	WDT
cord-271701-tx0lqgff	108	11	was	be	VBD
cord-271701-tx0lqgff	108	12	subsequently	subsequently	RB
cord-271701-tx0lqgff	108	13	processed	process	VBN
cord-271701-tx0lqgff	108	14	at	at	IN
cord-271701-tx0lqgff	108	15	both	both	DT
cord-271701-tx0lqgff	108	16	termini	terminus	NNS
cord-271701-tx0lqgff	108	17	in	in	IN
cord-271701-tx0lqgff	108	18	two	two	CD
cord-271701-tx0lqgff	108	19	steps	step	NNS
cord-271701-tx0lqgff	108	20	.	.	.
cord-271701-tx0lqgff	109	1	The	the	DT
cord-271701-tx0lqgff	109	2	first	first	JJ
cord-271701-tx0lqgff	109	3	step	step	NN
cord-271701-tx0lqgff	109	4	was	be	VBD
cord-271701-tx0lqgff	109	5	co	co	JJ
cord-271701-tx0lqgff	109	6	-	-	JJ
cord-271701-tx0lqgff	109	7	translational	translational	JJ
cord-271701-tx0lqgff	109	8	and	and	CC
cord-271701-tx0lqgff	109	9	involved	involve	VBD
cord-271701-tx0lqgff	109	10	the	the	DT
cord-271701-tx0lqgff	109	11	release	release	NN
cord-271701-tx0lqgff	109	12	of	of	IN
cord-271701-tx0lqgff	109	13	the	the	DT
cord-271701-tx0lqgff	109	14	N	n	CD
cord-271701-tx0lqgff	109	15	-	-	HYPH
cord-271701-tx0lqgff	109	16	terminal	terminal	NNP
cord-271701-tx0lqgff	109	17	ub	ub	NNP
cord-271701-tx0lqgff	109	18	fusion	fusion	NN
cord-271701-tx0lqgff	109	19	partner	partner	NN
cord-271701-tx0lqgff	109	20	by	by	IN
cord-271701-tx0lqgff	109	21	the	the	DT
cord-271701-tx0lqgff	109	22	co	co	NN
cord-271701-tx0lqgff	109	23	-	-	VBD
cord-271701-tx0lqgff	109	24	expressed	express	VBN
cord-271701-tx0lqgff	109	25	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	109	26	carboxyl	carboxyl	NN
cord-271701-tx0lqgff	109	27	-	-	HYPH
cord-271701-tx0lqgff	109	28	terminal	terminal	NN
cord-271701-tx0lqgff	109	29	hydrolase	hydrolase	NN
cord-271701-tx0lqgff	109	30	1	1	CD
cord-271701-tx0lqgff	109	31	(	(	-LRB-
cord-271701-tx0lqgff	109	32	Upb1	Upb1	NNP
cord-271701-tx0lqgff	109	33	,	,	,
cord-271701-tx0lqgff	109	34	Figure	figure	NN
cord-271701-tx0lqgff	109	35	2A	2a	CD
cord-271701-tx0lqgff	109	36	)	)	-RRB-
cord-271701-tx0lqgff	109	37	(	(	-LRB-
cord-271701-tx0lqgff	109	38	10	10	CD
cord-271701-tx0lqgff	109	39	,	,	,
cord-271701-tx0lqgff	109	40	14	14	CD
cord-271701-tx0lqgff	109	41	)	)	-RRB-
cord-271701-tx0lqgff	109	42	.	.	.
cord-271701-tx0lqgff	110	1	The	the	DT
cord-271701-tx0lqgff	110	2	second	second	JJ
cord-271701-tx0lqgff	110	3	proteolytic	proteolytic	JJ
cord-271701-tx0lqgff	110	4	step	step	NN
cord-271701-tx0lqgff	110	5	,	,	,
cord-271701-tx0lqgff	110	6	catalysed	catalyse	VBN
cord-271701-tx0lqgff	110	7	by	by	IN
cord-271701-tx0lqgff	110	8	a	a	DT
cord-271701-tx0lqgff	110	9	recombinant	recombinant	JJ
cord-271701-tx0lqgff	110	10	form	form	NN
cord-271701-tx0lqgff	110	11	of	of	IN
cord-271701-tx0lqgff	110	12	the	the	DT
cord-271701-tx0lqgff	110	13	SARS	SARS	NNP
cord-271701-tx0lqgff	110	14	-	-	HYPH
cord-271701-tx0lqgff	110	15	CoV	CoV	NNP
cord-271701-tx0lqgff	110	16	nsp5	nsp5	NN
cord-271701-tx0lqgff	110	17	main	main	JJ
cord-271701-tx0lqgff	110	18	protease	protease	NN
cord-271701-tx0lqgff	110	19	(	(	-LRB-
cord-271701-tx0lqgff	110	20	15	15	CD
cord-271701-tx0lqgff	110	21	)	)	-RRB-
cord-271701-tx0lqgff	110	22	,	,	,
cord-271701-tx0lqgff	110	23	removed	remove	VBD
cord-271701-tx0lqgff	110	24	the	the	DT
cord-271701-tx0lqgff	110	25	C	c	NN
cord-271701-tx0lqgff	110	26	-	-	HYPH
cord-271701-tx0lqgff	110	27	terminal	terminal	NN
cord-271701-tx0lqgff	111	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	111	2	6	6	CD
cord-271701-tx0lqgff	111	3	-tag	-tag	RBS
cord-271701-tx0lqgff	111	4	and	and	CC
cord-271701-tx0lqgff	111	5	was	be	VBD
cord-271701-tx0lqgff	111	6	performed	perform	VBN
cord-271701-tx0lqgff	111	7	either	either	CC
cord-271701-tx0lqgff	111	8	in	in	IN
cord-271701-tx0lqgff	111	9	solution	solution	NN
cord-271701-tx0lqgff	111	10	(	(	-LRB-
cord-271701-tx0lqgff	111	11	Figure	figure	NN
cord-271701-tx0lqgff	111	12	2A	2a	NN
cord-271701-tx0lqgff	111	13	and	and	CC
cord-271701-tx0lqgff	111	14	B	b	NN
cord-271701-tx0lqgff	111	15	)	)	-RRB-
cord-271701-tx0lqgff	111	16	or	or	CC
cord-271701-tx0lqgff	111	17	when	when	WRB
cord-271701-tx0lqgff	111	18	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	111	19	was	be	VBD
cord-271701-tx0lqgff	111	20	immobilised	immobilise	VBN
cord-271701-tx0lqgff	111	21	to	to	IN
cord-271701-tx0lqgff	111	22	Talon	Talon	NNP
cord-271701-tx0lqgff	111	23	beads	bead	NNS
cord-271701-tx0lqgff	111	24	.	.	.
cord-271701-tx0lqgff	112	1	This	this	DT
cord-271701-tx0lqgff	112	2	procedure	procedure	NN
cord-271701-tx0lqgff	112	3	yielded	yield	VBD
cord-271701-tx0lqgff	112	4	SARS	SARS	NNP
cord-271701-tx0lqgff	112	5	-	-	HYPH
cord-271701-tx0lqgff	112	6	CoV	CoV	NNP
cord-271701-tx0lqgff	112	7	nsp8	nsp8	NN
cord-271701-tx0lqgff	112	8	with	with	IN
cord-271701-tx0lqgff	112	9	its	-PRON-	PRP$
cord-271701-tx0lqgff	112	10	exact	exact	JJ
cord-271701-tx0lqgff	112	11	natural	natural	JJ
cord-271701-tx0lqgff	112	12	Nand	Nand	NNP
cord-271701-tx0lqgff	112	13	C	C	NNP
cord-271701-tx0lqgff	112	14	-	-	HYPH
cord-271701-tx0lqgff	112	15	terminus	terminus	NNP
cord-271701-tx0lqgff	112	16	(	(	-LRB-
cord-271701-tx0lqgff	112	17	replicase	replicase	NN
cord-271701-tx0lqgff	112	18	residues	residue	NNS
cord-271701-tx0lqgff	112	19	Ala-3920	Ala-3920	NNP
cord-271701-tx0lqgff	112	20	and	and	CC
cord-271701-tx0lqgff	112	21	Gln-4117	gln-4117	NN
cord-271701-tx0lqgff	112	22	,	,	,
cord-271701-tx0lqgff	112	23	respectively	respectively	RB
cord-271701-tx0lqgff	112	24	;	;	:
cord-271701-tx0lqgff	112	25	Figure	figure	NN
cord-271701-tx0lqgff	112	26	2A	2a	CD
cord-271701-tx0lqgff	112	27	)	)	-RRB-
cord-271701-tx0lqgff	112	28	,	,	,
cord-271701-tx0lqgff	112	29	the	the	DT
cord-271701-tx0lqgff	112	30	product	product	NN
cord-271701-tx0lqgff	112	31	that	that	WDT
cord-271701-tx0lqgff	112	32	is	be	VBZ
cord-271701-tx0lqgff	112	33	normally	normally	RB
cord-271701-tx0lqgff	112	34	liberated	liberate	VBN
cord-271701-tx0lqgff	112	35	by	by	IN
cord-271701-tx0lqgff	112	36	the	the	DT
cord-271701-tx0lqgff	112	37	nsp5-driven	nsp5-driven	NNP
cord-271701-tx0lqgff	112	38	autoprocessing	autoprocesse	VBG
cord-271701-tx0lqgff	112	39	of	of	IN
cord-271701-tx0lqgff	112	40	the	the	DT
cord-271701-tx0lqgff	112	41	SARS	SARS	NNP
cord-271701-tx0lqgff	112	42	-	-	HYPH
cord-271701-tx0lqgff	112	43	CoV	CoV	NNP
cord-271701-tx0lqgff	112	44	replicase	replicase	NNP
cord-271701-tx0lqgff	112	45	polyproteins	polyproteins	NNP
cord-271701-tx0lqgff	112	46	(	(	-LRB-
cord-271701-tx0lqgff	112	47	19	19	CD
cord-271701-tx0lqgff	112	48	)	)	-RRB-
cord-271701-tx0lqgff	112	49	.	.	.
cord-271701-tx0lqgff	113	1	In	in	IN
cord-271701-tx0lqgff	113	2	accordance	accordance	NN
cord-271701-tx0lqgff	113	3	with	with	IN
cord-271701-tx0lqgff	113	4	the	the	DT
cord-271701-tx0lqgff	113	5	octameric	octameric	JJ
cord-271701-tx0lqgff	113	6	state	state	NN
cord-271701-tx0lqgff	113	7	observed	observe	VBN
cord-271701-tx0lqgff	113	8	in	in	IN
cord-271701-tx0lqgff	113	9	cross	cross	JJ
cord-271701-tx0lqgff	113	10	-	-	JJ
cord-271701-tx0lqgff	113	11	linking	link	VBG
cord-271701-tx0lqgff	113	12	experiments	experiment	NNS
cord-271701-tx0lqgff	113	13	using	use	VBG
cord-271701-tx0lqgff	113	14	glutaraldehyde	glutaraldehyde	VBN
cord-271701-tx0lqgff	113	15	(	(	-LRB-
cord-271701-tx0lqgff	113	16	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	113	17	Figure	Figure	NNP
cord-271701-tx0lqgff	113	18	S1	S1	NNP
cord-271701-tx0lqgff	113	19	)	)	-RRB-
cord-271701-tx0lqgff	113	20	or	or	CC
cord-271701-tx0lqgff	113	21	ethylene	ethylene	NN
cord-271701-tx0lqgff	113	22	glycolbis	glycolbis	NNP
cord-271701-tx0lqgff	113	23	(	(	-LRB-
cord-271701-tx0lqgff	113	24	13	13	CD
cord-271701-tx0lqgff	113	25	)	)	-RRB-
cord-271701-tx0lqgff	113	26	,	,	,
cord-271701-tx0lqgff	113	27	the	the	DT
cord-271701-tx0lqgff	113	28	hydrodynamic	hydrodynamic	JJ
cord-271701-tx0lqgff	113	29	profile	profile	NN
cord-271701-tx0lqgff	113	30	of	of	IN
cord-271701-tx0lqgff	113	31	the	the	DT
cord-271701-tx0lqgff	113	32	untagged	untagged	JJ
cord-271701-tx0lqgff	113	33	nsp8	nsp8	NN
cord-271701-tx0lqgff	113	34	corresponded	correspond	VBD
cord-271701-tx0lqgff	113	35	to	to	IN
cord-271701-tx0lqgff	113	36	a	a	DT
cord-271701-tx0lqgff	113	37	mass	mass	NN
cord-271701-tx0lqgff	113	38	of	of	IN
cord-271701-tx0lqgff	113	39	$	$	$
cord-271701-tx0lqgff	113	40	160	160	CD
cord-271701-tx0lqgff	113	41	kDa	kDa	NNS
cord-271701-tx0lqgff	113	42	(	(	-LRB-
cord-271701-tx0lqgff	113	43	Figure	Figure	NNP
cord-271701-tx0lqgff	113	44	2D	2D	NNP
cord-271701-tx0lqgff	113	45	)	)	-RRB-
cord-271701-tx0lqgff	113	46	.	.	.
cord-271701-tx0lqgff	114	1	To	to	TO
cord-271701-tx0lqgff	114	2	identify	identify	VB
cord-271701-tx0lqgff	114	3	and	and	CC
cord-271701-tx0lqgff	114	4	explain	explain	VB
cord-271701-tx0lqgff	114	5	differences	difference	NNS
cord-271701-tx0lqgff	114	6	with	with	IN
cord-271701-tx0lqgff	114	7	previously	previously	RB
cord-271701-tx0lqgff	114	8	published	publish	VBN
cord-271701-tx0lqgff	114	9	observations	observation	NNS
cord-271701-tx0lqgff	114	10	,	,	,
cord-271701-tx0lqgff	114	11	we	-PRON-	PRP
cord-271701-tx0lqgff	114	12	also	also	RB
cord-271701-tx0lqgff	114	13	produced	produce	VBD
cord-271701-tx0lqgff	114	14	and	and	CC
cord-271701-tx0lqgff	114	15	characterised	characterise	VBN
cord-271701-tx0lqgff	114	16	N	n	CD
cord-271701-tx0lqgff	114	17	-	-	HYPH
cord-271701-tx0lqgff	114	18	and	and	CC
cord-271701-tx0lqgff	114	19	C	C	NNP
cord-271701-tx0lqgff	114	20	-	-	HYPH
cord-271701-tx0lqgff	114	21	terminally	terminally	RB
cord-271701-tx0lqgff	114	22	tagged	tag	VBN
cord-271701-tx0lqgff	114	23	forms	form	NNS
cord-271701-tx0lqgff	114	24	of	of	IN
cord-271701-tx0lqgff	114	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	114	26	(	(	-LRB-
cord-271701-tx0lqgff	114	27	Figure	figure	NN
cord-271701-tx0lqgff	114	28	2C	2c	NN
cord-271701-tx0lqgff	114	29	)	)	-RRB-
cord-271701-tx0lqgff	114	30	.	.	.
cord-271701-tx0lqgff	115	1	Importantly	importantly	RB
cord-271701-tx0lqgff	115	2	,	,	,
cord-271701-tx0lqgff	115	3	under	under	IN
cord-271701-tx0lqgff	115	4	the	the	DT
cord-271701-tx0lqgff	115	5	same	same	JJ
cord-271701-tx0lqgff	115	6	assay	assay	NN
cord-271701-tx0lqgff	115	7	conditions	condition	NNS
cord-271701-tx0lqgff	115	8	,	,	,
cord-271701-tx0lqgff	115	9	the	the	DT
cord-271701-tx0lqgff	115	10	N	n	NN
cord-271701-tx0lqgff	115	11	-	-	HYPH
cord-271701-tx0lqgff	115	12	terminally	terminally	RB
cord-271701-tx0lqgff	115	13	His	-PRON-	PRP$
cord-271701-tx0lqgff	115	14	6	6	CD
cord-271701-tx0lqgff	115	15	-tagged	-tagged	CD
cord-271701-tx0lqgff	115	16	nsp8	nsp8	CD
cord-271701-tx0lqgff	115	17	(	(	-LRB-
cord-271701-tx0lqgff	115	18	His	-PRON-	PRP$
cord-271701-tx0lqgff	115	19	-	-	HYPH
cord-271701-tx0lqgff	115	20	nsp8	nsp8	NN
cord-271701-tx0lqgff	115	21	)	)	-RRB-
cord-271701-tx0lqgff	115	22	that	that	WDT
cord-271701-tx0lqgff	115	23	was	be	VBD
cord-271701-tx0lqgff	115	24	used	use	VBN
cord-271701-tx0lqgff	115	25	in	in	IN
cord-271701-tx0lqgff	115	26	the	the	DT
cord-271701-tx0lqgff	115	27	original	original	JJ
cord-271701-tx0lqgff	115	28	nsp8	nsp8	NNP
cord-271701-tx0lqgff	115	29	RdRp	RdRp	NNP
cord-271701-tx0lqgff	115	30	activity	activity	NN
cord-271701-tx0lqgff	115	31	study	study	NN
cord-271701-tx0lqgff	115	32	(	(	-LRB-
cord-271701-tx0lqgff	115	33	12	12	CD
cord-271701-tx0lqgff	115	34	)	)	-RRB-
cord-271701-tx0lqgff	115	35	showed	show	VBD
cord-271701-tx0lqgff	115	36	a	a	DT
cord-271701-tx0lqgff	115	37	marked	marked	JJ
cord-271701-tx0lqgff	115	38	difference	difference	NN
cord-271701-tx0lqgff	115	39	in	in	IN
cord-271701-tx0lqgff	115	40	multimerization	multimerization	NN
cord-271701-tx0lqgff	115	41	behaviour	behaviour	NN
cord-271701-tx0lqgff	115	42	(	(	-LRB-
cord-271701-tx0lqgff	115	43	Figure	figure	NN
cord-271701-tx0lqgff	115	44	2D	2D	NNP
cord-271701-tx0lqgff	115	45	and	and	CC
cord-271701-tx0lqgff	115	46	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	115	47	Figure	Figure	NNP
cord-271701-tx0lqgff	115	48	S1	S1	NNP
cord-271701-tx0lqgff	115	49	)	)	-RRB-
cord-271701-tx0lqgff	115	50	.	.	.
cord-271701-tx0lqgff	116	1	On	on	IN
cord-271701-tx0lqgff	116	2	the	the	DT
cord-271701-tx0lqgff	116	3	other	other	JJ
cord-271701-tx0lqgff	116	4	hand	hand	NN
cord-271701-tx0lqgff	116	5	,	,	,
cord-271701-tx0lqgff	116	6	little	little	JJ
cord-271701-tx0lqgff	116	7	difference	difference	NN
cord-271701-tx0lqgff	116	8	was	be	VBD
cord-271701-tx0lqgff	116	9	observed	observe	VBN
cord-271701-tx0lqgff	116	10	between	between	IN
cord-271701-tx0lqgff	116	11	untagged	untagged	JJ
cord-271701-tx0lqgff	116	12	nsp8	nsp8	NNP
cord-271701-tx0lqgff	116	13	and	and	CC
cord-271701-tx0lqgff	116	14	a	a	DT
cord-271701-tx0lqgff	116	15	C	c	NN
cord-271701-tx0lqgff	116	16	-	-	HYPH
cord-271701-tx0lqgff	116	17	terminally	terminally	RB
cord-271701-tx0lqgff	116	18	His	-PRON-	PRP$
cord-271701-tx0lqgff	116	19	6	6	CD
cord-271701-tx0lqgff	116	20	-tagged	-tagged	JJ
cord-271701-tx0lqgff	116	21	version	version	NN
cord-271701-tx0lqgff	116	22	of	of	IN
cord-271701-tx0lqgff	116	23	the	the	DT
cord-271701-tx0lqgff	116	24	protein	protein	NN
cord-271701-tx0lqgff	116	25	(	(	-LRB-
cord-271701-tx0lqgff	116	26	nsp8-His	nsp8-his	UH
cord-271701-tx0lqgff	116	27	;	;	:
cord-271701-tx0lqgff	116	28	Figure	figure	NN
cord-271701-tx0lqgff	116	29	2E	2e	NN
cord-271701-tx0lqgff	116	30	)	)	-RRB-
cord-271701-tx0lqgff	116	31	.	.	.
cord-271701-tx0lqgff	117	1	To	to	TO
cord-271701-tx0lqgff	117	2	investigate	investigate	VB
cord-271701-tx0lqgff	117	3	whether	whether	IN
cord-271701-tx0lqgff	117	4	nsp7	nsp7	PRP
cord-271701-tx0lqgff	117	5	could	could	MD
cord-271701-tx0lqgff	117	6	influence	influence	VB
cord-271701-tx0lqgff	117	7	the	the	DT
cord-271701-tx0lqgff	117	8	change	change	NN
cord-271701-tx0lqgff	117	9	in	in	IN
cord-271701-tx0lqgff	117	10	multimerisation	multimerisation	NN
cord-271701-tx0lqgff	117	11	behaviour	behaviour	NN
cord-271701-tx0lqgff	117	12	,	,	,
cord-271701-tx0lqgff	117	13	we	-PRON-	PRP
cord-271701-tx0lqgff	117	14	next	next	RB
cord-271701-tx0lqgff	117	15	added	add	VBD
cord-271701-tx0lqgff	117	16	separately	separately	RB
cord-271701-tx0lqgff	117	17	purified	purify	VBN
cord-271701-tx0lqgff	117	18	and	and	CC
cord-271701-tx0lqgff	117	19	C	c	NN
cord-271701-tx0lqgff	117	20	-	-	,
cord-271701-tx0lqgff	117	21	terminally	terminally	RB
cord-271701-tx0lqgff	117	22	processed	process	VBN
cord-271701-tx0lqgff	117	23	nsp7	nsp7	PRP
cord-271701-tx0lqgff	117	24	to	to	IN
cord-271701-tx0lqgff	117	25	the	the	DT
cord-271701-tx0lqgff	117	26	different	different	JJ
cord-271701-tx0lqgff	117	27	nsp8	nsp8	JJ
cord-271701-tx0lqgff	117	28	preparations	preparation	NNS
cord-271701-tx0lqgff	117	29	.	.	.
cord-271701-tx0lqgff	118	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	118	2	,	,	,
cord-271701-tx0lqgff	118	3	we	-PRON-	PRP
cord-271701-tx0lqgff	118	4	found	find	VBD
cord-271701-tx0lqgff	118	5	that	that	IN
cord-271701-tx0lqgff	118	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	118	7	and	and	CC
cord-271701-tx0lqgff	118	8	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	118	9	could	could	MD
cord-271701-tx0lqgff	118	10	both	both	DT
cord-271701-tx0lqgff	118	11	associate	associate	VB
cord-271701-tx0lqgff	118	12	with	with	IN
cord-271701-tx0lqgff	118	13	this	this	DT
cord-271701-tx0lqgff	118	14	protein	protein	NN
cord-271701-tx0lqgff	118	15	,	,	,
cord-271701-tx0lqgff	118	16	in	in	IN
cord-271701-tx0lqgff	118	17	accordance	accordance	NN
cord-271701-tx0lqgff	118	18	with	with	IN
cord-271701-tx0lqgff	118	19	published	publish	VBN
cord-271701-tx0lqgff	118	20	data	datum	NNS
cord-271701-tx0lqgff	118	21	(	(	-LRB-
cord-271701-tx0lqgff	118	22	13	13	CD
cord-271701-tx0lqgff	118	23	)	)	-RRB-
cord-271701-tx0lqgff	118	24	,	,	,
cord-271701-tx0lqgff	118	25	but	but	CC
cord-271701-tx0lqgff	118	26	that	that	IN
cord-271701-tx0lqgff	118	27	His	-PRON-	PRP$
cord-271701-tx0lqgff	118	28	-	-	HYPH
cord-271701-tx0lqgff	118	29	nsp8	nsp8	NN
cord-271701-tx0lqgff	118	30	was	be	VBD
cord-271701-tx0lqgff	118	31	unable	unable	JJ
cord-271701-tx0lqgff	118	32	to	to	TO
cord-271701-tx0lqgff	118	33	do	do	VB
cord-271701-tx0lqgff	118	34	so	so	RB
cord-271701-tx0lqgff	118	35	within	within	IN
cord-271701-tx0lqgff	118	36	the	the	DT
cord-271701-tx0lqgff	118	37	frame	frame	NN
cord-271701-tx0lqgff	118	38	of	of	IN
cord-271701-tx0lqgff	118	39	our	-PRON-	PRP$
cord-271701-tx0lqgff	118	40	experimental	experimental	JJ
cord-271701-tx0lqgff	118	41	conditions	condition	NNS
cord-271701-tx0lqgff	118	42	(	(	-LRB-
cord-271701-tx0lqgff	118	43	Figure	figure	NN
cord-271701-tx0lqgff	118	44	2F	2f	NN
cord-271701-tx0lqgff	118	45	)	)	-RRB-
cord-271701-tx0lqgff	118	46	.	.	.
cord-271701-tx0lqgff	119	1	Consequently	consequently	RB
cord-271701-tx0lqgff	119	2	,	,	,
cord-271701-tx0lqgff	119	3	although	although	IN
cord-271701-tx0lqgff	119	4	various	various	JJ
cord-271701-tx0lqgff	119	5	lines	line	NNS
cord-271701-tx0lqgff	119	6	of	of	IN
cord-271701-tx0lqgff	119	7	evidence	evidence	NN
cord-271701-tx0lqgff	119	8	support	support	VBP
cord-271701-tx0lqgff	119	9	the	the	DT
cord-271701-tx0lqgff	119	10	observation	observation	NN
cord-271701-tx0lqgff	119	11	that	that	IN
cord-271701-tx0lqgff	119	12	nsp7	nsp7	NNP
cord-271701-tx0lqgff	119	13	and	and	CC
cord-271701-tx0lqgff	119	14	nsp8	nsp8	NN
cord-271701-tx0lqgff	119	15	can	can	MD
cord-271701-tx0lqgff	119	16	form	form	VB
cord-271701-tx0lqgff	119	17	a	a	DT
cord-271701-tx0lqgff	119	18	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	119	19	,	,	,
cord-271701-tx0lqgff	119	20	it	-PRON-	PRP
cord-271701-tx0lqgff	119	21	now	now	RB
cord-271701-tx0lqgff	119	22	appears	appear	VBZ
cord-271701-tx0lqgff	119	23	that	that	IN
cord-271701-tx0lqgff	119	24	the	the	DT
cord-271701-tx0lqgff	119	25	correct	correct	JJ
cord-271701-tx0lqgff	119	26	N	n	CD
cord-271701-tx0lqgff	119	27	-	-	HYPH
cord-271701-tx0lqgff	119	28	terminal	terminal	NN
cord-271701-tx0lqgff	119	29	processing	processing	NN
cord-271701-tx0lqgff	119	30	of	of	IN
cord-271701-tx0lqgff	119	31	nsp8	nsp8	NN
cord-271701-tx0lqgff	119	32	is	be	VBZ
cord-271701-tx0lqgff	119	33	a	a	DT
cord-271701-tx0lqgff	119	34	significant	significant	JJ
cord-271701-tx0lqgff	119	35	factor	factor	NN
cord-271701-tx0lqgff	119	36	in	in	IN
cord-271701-tx0lqgff	119	37	determining	determine	VBG
cord-271701-tx0lqgff	119	38	the	the	DT
cord-271701-tx0lqgff	119	39	final	final	JJ
cord-271701-tx0lqgff	119	40	oligomeric	oligomeric	JJ
cord-271701-tx0lqgff	119	41	state	state	NN
cord-271701-tx0lqgff	119	42	of	of	IN
cord-271701-tx0lqgff	119	43	the	the	DT
cord-271701-tx0lqgff	119	44	protein	protein	NN
cord-271701-tx0lqgff	119	45	.	.	.
cord-271701-tx0lqgff	120	1	A	a	DT
cord-271701-tx0lqgff	120	2	unique	unique	JJ
cord-271701-tx0lqgff	120	3	feature	feature	NN
cord-271701-tx0lqgff	120	4	of	of	IN
cord-271701-tx0lqgff	120	5	the	the	DT
cord-271701-tx0lqgff	120	6	hexadecameric	hexadecameric	NNP
cord-271701-tx0lqgff	120	7	SARS	SARS	NNP
cord-271701-tx0lqgff	120	8	-	-	HYPH
cord-271701-tx0lqgff	120	9	CoV	CoV	NNP
cord-271701-tx0lqgff	120	10	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	120	11	+	+	SYM
cord-271701-tx0lqgff	120	12	8	8	CD
cord-271701-tx0lqgff	120	13	)	)	-RRB-
cord-271701-tx0lqgff	120	14	structure	structure	NN
cord-271701-tx0lqgff	120	15	is	be	VBZ
cord-271701-tx0lqgff	120	16	the	the	DT
cord-271701-tx0lqgff	120	17	fact	fact	NN
cord-271701-tx0lqgff	120	18	that	that	IN
cord-271701-tx0lqgff	120	19	it	-PRON-	PRP
cord-271701-tx0lqgff	120	20	does	do	VBZ
cord-271701-tx0lqgff	120	21	not	not	RB
cord-271701-tx0lqgff	120	22	derive	derive	VB
cord-271701-tx0lqgff	120	23	from	from	IN
cord-271701-tx0lqgff	120	24	stacking	stack	VBG
cord-271701-tx0lqgff	120	25	of	of	IN
cord-271701-tx0lqgff	120	26	its	-PRON-	PRP$
cord-271701-tx0lqgff	120	27	protein	protein	NN
cord-271701-tx0lqgff	120	28	subunits	subunit	NNS
cord-271701-tx0lqgff	120	29	,	,	,
cord-271701-tx0lqgff	120	30	but	but	CC
cord-271701-tx0lqgff	120	31	rather	rather	RB
cord-271701-tx0lqgff	120	32	from	from	IN
cord-271701-tx0lqgff	120	33	stable	stable	JJ
cord-271701-tx0lqgff	120	34	inter	inter	JJ
cord-271701-tx0lqgff	120	35	-	-	NNS
cord-271701-tx0lqgff	120	36	connections	connection	NNS
cord-271701-tx0lqgff	120	37	of	of	IN
cord-271701-tx0lqgff	120	38	the	the	DT
cord-271701-tx0lqgff	120	39	'	'	``
cord-271701-tx0lqgff	120	40	golf	golf	NN
cord-271701-tx0lqgff	120	41	club	club	NN
cord-271701-tx0lqgff	120	42	-	-	HYPH
cord-271701-tx0lqgff	120	43	like	like	JJ
cord-271701-tx0lqgff	120	44	'	'	''
cord-271701-tx0lqgff	120	45	nsp8	nsp8	JJ
cord-271701-tx0lqgff	120	46	molecules	molecule	NNS
cord-271701-tx0lqgff	120	47	(	(	-LRB-
cord-271701-tx0lqgff	120	48	Figure	figure	NN
cord-271701-tx0lqgff	120	49	1B	1b	CD
cord-271701-tx0lqgff	120	50	)	)	-RRB-
cord-271701-tx0lqgff	120	51	(	(	-LRB-
cord-271701-tx0lqgff	120	52	13	13	CD
cord-271701-tx0lqgff	120	53	)	)	-RRB-
cord-271701-tx0lqgff	120	54	.	.	.
cord-271701-tx0lqgff	121	1	The	the	DT
cord-271701-tx0lqgff	121	2	structural	structural	JJ
cord-271701-tx0lqgff	121	3	support	support	NN
cord-271701-tx0lqgff	121	4	of	of	IN
cord-271701-tx0lqgff	121	5	the	the	DT
cord-271701-tx0lqgff	121	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	121	7	,	,	,
cord-271701-tx0lqgff	121	8	followed	follow	VBN
cord-271701-tx0lqgff	121	9	by	by	IN
cord-271701-tx0lqgff	121	10	purification	purification	NN
cord-271701-tx0lqgff	121	11	and	and	CC
cord-271701-tx0lqgff	121	12	cleavage	cleavage	NN
cord-271701-tx0lqgff	121	13	by	by	IN
cord-271701-tx0lqgff	121	14	recombinant	recombinant	JJ
cord-271701-tx0lqgff	121	15	SARS	SARS	NNP
cord-271701-tx0lqgff	121	16	-	-	HYPH
cord-271701-tx0lqgff	121	17	CoV	CoV	NNP
cord-271701-tx0lqgff	121	18	nsp5	nsp5	NN
cord-271701-tx0lqgff	121	19	main	main	JJ
cord-271701-tx0lqgff	121	20	protease	protease	NN
cord-271701-tx0lqgff	121	21	to	to	TO
cord-271701-tx0lqgff	121	22	remove	remove	VB
cord-271701-tx0lqgff	121	23	the	the	DT
cord-271701-tx0lqgff	121	24	C	c	NN
cord-271701-tx0lqgff	121	25	-	-	HYPH
cord-271701-tx0lqgff	121	26	terminal	terminal	NN
cord-271701-tx0lqgff	122	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	122	2	6	6	CD
cord-271701-tx0lqgff	122	3	-tag	-tag	RBS
cord-271701-tx0lqgff	122	4	and	and	CC
cord-271701-tx0lqgff	122	5	its	-PRON-	PRP$
cord-271701-tx0lqgff	122	6	upstream	upstream	JJ
cord-271701-tx0lqgff	122	7	GSSG	GSSG	NNP
cord-271701-tx0lqgff	122	8	linker	linker	NN
cord-271701-tx0lqgff	122	9	.	.	.
cord-271701-tx0lqgff	123	1	(	(	-LRB-
cord-271701-tx0lqgff	123	2	B	b	NN
cord-271701-tx0lqgff	123	3	)	)	-RRB-
cord-271701-tx0lqgff	124	1	Eighteen	eighteen	CD
cord-271701-tx0lqgff	124	2	percent	percent	NN
cord-271701-tx0lqgff	124	3	SDS	SDS	NNP
cord-271701-tx0lqgff	124	4	-	-	HYPH
cord-271701-tx0lqgff	124	5	PAGE	PAGE	NNP
cord-271701-tx0lqgff	124	6	analysis	analysis	NN
cord-271701-tx0lqgff	124	7	of	of	IN
cord-271701-tx0lqgff	124	8	nsp5-treated	nsp5-treate	VBN
cord-271701-tx0lqgff	124	9	,	,	,
cord-271701-tx0lqgff	124	10	purified	purify	VBN
cord-271701-tx0lqgff	125	1	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	125	2	demonstrates	demonstrate	VBZ
cord-271701-tx0lqgff	125	3	near	near	RB
cord-271701-tx0lqgff	125	4	-	-	HYPH
cord-271701-tx0lqgff	125	5	complete	complete	JJ
cord-271701-tx0lqgff	125	6	release	release	NN
cord-271701-tx0lqgff	125	7	of	of	IN
cord-271701-tx0lqgff	125	8	the	the	DT
cord-271701-tx0lqgff	125	9	C	C	NNP
cord-271701-tx0lqgff	125	10	-	-	HYPH
cord-271701-tx0lqgff	125	11	terminal	terminal	NN
cord-271701-tx0lqgff	126	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	126	2	6	6	CD
cord-271701-tx0lqgff	126	3	-tag	-tag	RB
cord-271701-tx0lqgff	126	4	within	within	IN
cord-271701-tx0lqgff	126	5	60	60	CD
cord-271701-tx0lqgff	126	6	min	min	NN
cord-271701-tx0lqgff	126	7	.	.	.
cord-271701-tx0lqgff	127	1	The	the	DT
cord-271701-tx0lqgff	127	2	maltose	maltose	NN
cord-271701-tx0lqgff	127	3	binding	bind	VBG
cord-271701-tx0lqgff	127	4	protein	protein	NN
cord-271701-tx0lqgff	127	5	(	(	-LRB-
cord-271701-tx0lqgff	127	6	MBP	MBP	NNP
cord-271701-tx0lqgff	127	7	)	)	-RRB-
cord-271701-tx0lqgff	127	8	was	be	VBD
cord-271701-tx0lqgff	127	9	added	add	VBN
cord-271701-tx0lqgff	127	10	to	to	IN
cord-271701-tx0lqgff	127	11	the	the	DT
cord-271701-tx0lqgff	127	12	reaction	reaction	NN
cord-271701-tx0lqgff	127	13	to	to	TO
cord-271701-tx0lqgff	127	14	serve	serve	VB
cord-271701-tx0lqgff	127	15	as	as	IN
cord-271701-tx0lqgff	127	16	an	an	DT
cord-271701-tx0lqgff	127	17	independent	independent	JJ
cord-271701-tx0lqgff	127	18	loading	loading	NN
cord-271701-tx0lqgff	127	19	control	control	NN
cord-271701-tx0lqgff	127	20	.	.	.
cord-271701-tx0lqgff	128	1	Asterisks	asterisk	NNS
cord-271701-tx0lqgff	128	2	indicate	indicate	VBP
cord-271701-tx0lqgff	128	3	non	non	JJ
cord-271701-tx0lqgff	128	4	-	-	JJ
cord-271701-tx0lqgff	128	5	specific	specific	JJ
cord-271701-tx0lqgff	128	6	bands	band	NNS
cord-271701-tx0lqgff	128	7	.	.	.
cord-271701-tx0lqgff	129	1	(	(	-LRB-
cord-271701-tx0lqgff	129	2	C	C	NNP
cord-271701-tx0lqgff	129	3	)	)	-RRB-
cord-271701-tx0lqgff	130	1	In	in	IN
cord-271701-tx0lqgff	130	2	addition	addition	NN
cord-271701-tx0lqgff	130	3	to	to	IN
cord-271701-tx0lqgff	130	4	the	the	DT
cord-271701-tx0lqgff	130	5	tag	tag	NN
cord-271701-tx0lqgff	130	6	-	-	HYPH
cord-271701-tx0lqgff	130	7	less	less	JJR
cord-271701-tx0lqgff	130	8	nps8	nps8	NN
cord-271701-tx0lqgff	130	9	and	and	CC
cord-271701-tx0lqgff	130	10	nsp8-His	nsp8-his	UH
cord-271701-tx0lqgff	130	11	,	,	,
cord-271701-tx0lqgff	130	12	we	-PRON-	PRP
cord-271701-tx0lqgff	130	13	also	also	RB
cord-271701-tx0lqgff	130	14	produced	produce	VBD
cord-271701-tx0lqgff	130	15	the	the	DT
cord-271701-tx0lqgff	130	16	N	n	NN
cord-271701-tx0lqgff	130	17	-	-	HYPH
cord-271701-tx0lqgff	130	18	terminally	terminally	RB
cord-271701-tx0lqgff	130	19	His	-PRON-	PRP$
cord-271701-tx0lqgff	130	20	6	6	CD
cord-271701-tx0lqgff	130	21	-tagged	-tagged	CD
cord-271701-tx0lqgff	130	22	nsp8	nsp8	CD
cord-271701-tx0lqgff	130	23	(	(	-LRB-
cord-271701-tx0lqgff	130	24	His	-PRON-	PRP$
cord-271701-tx0lqgff	130	25	-	-	HYPH
cord-271701-tx0lqgff	130	26	nsp8	nsp8	NN
cord-271701-tx0lqgff	130	27	)	)	-RRB-
cord-271701-tx0lqgff	130	28	used	use	VBN
cord-271701-tx0lqgff	130	29	by	by	IN
cord-271701-tx0lqgff	130	30	Imbert	Imbert	NNP
cord-271701-tx0lqgff	130	31	et	et	NNP
cord-271701-tx0lqgff	130	32	al	al	NNP
cord-271701-tx0lqgff	130	33	.	.	.
cord-271701-tx0lqgff	131	1	(	(	-LRB-
cord-271701-tx0lqgff	131	2	12	12	CD
cord-271701-tx0lqgff	131	3	)	)	-RRB-
cord-271701-tx0lqgff	131	4	.	.	.
cord-271701-tx0lqgff	132	1	(	(	-LRB-
cord-271701-tx0lqgff	132	2	D	D	NNP
cord-271701-tx0lqgff	132	3	)	)	-RRB-
cord-271701-tx0lqgff	132	4	Comparative	comparative	JJ
cord-271701-tx0lqgff	132	5	gel	gel	NN
cord-271701-tx0lqgff	132	6	filtration	filtration	NN
cord-271701-tx0lqgff	132	7	analysis	analysis	NN
cord-271701-tx0lqgff	132	8	of	of	IN
cord-271701-tx0lqgff	132	9	nsp8	nsp8	NN
cord-271701-tx0lqgff	132	10	(	(	-LRB-
cord-271701-tx0lqgff	132	11	22	22	CD
cord-271701-tx0lqgff	132	12	kDa	kDa	NNS
cord-271701-tx0lqgff	132	13	as	as	IN
cord-271701-tx0lqgff	132	14	a	a	DT
cord-271701-tx0lqgff	132	15	monomer	monomer	NN
cord-271701-tx0lqgff	132	16	)	)	-RRB-
cord-271701-tx0lqgff	132	17	versus	versus	IN
cord-271701-tx0lqgff	132	18	His	-PRON-	PRP$
cord-271701-tx0lqgff	132	19	-	-	HYPH
cord-271701-tx0lqgff	132	20	nsp8	nsp8	NN
cord-271701-tx0lqgff	132	21	and	and	CC
cord-271701-tx0lqgff	132	22	(	(	-LRB-
cord-271701-tx0lqgff	132	23	E	e	NN
cord-271701-tx0lqgff	132	24	)	)	-RRB-
cord-271701-tx0lqgff	132	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	132	26	versus	versus	IN
cord-271701-tx0lqgff	132	27	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	132	28	.	.	.
cord-271701-tx0lqgff	133	1	In	in	IN
cord-271701-tx0lqgff	133	2	all	all	DT
cord-271701-tx0lqgff	133	3	three	three	CD
cord-271701-tx0lqgff	133	4	cases	case	NNS
cord-271701-tx0lqgff	133	5	,	,	,
cord-271701-tx0lqgff	133	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	133	7	formed	form	VBN
cord-271701-tx0lqgff	133	8	multimers	multimer	NNS
cord-271701-tx0lqgff	133	9	in	in	IN
cord-271701-tx0lqgff	133	10	solution	solution	NN
cord-271701-tx0lqgff	133	11	,	,	,
cord-271701-tx0lqgff	133	12	but	but	CC
cord-271701-tx0lqgff	133	13	the	the	DT
cord-271701-tx0lqgff	133	14	apparent	apparent	JJ
cord-271701-tx0lqgff	133	15	molecular	molecular	JJ
cord-271701-tx0lqgff	133	16	mass	mass	NN
cord-271701-tx0lqgff	133	17	of	of	IN
cord-271701-tx0lqgff	133	18	complexes	complex	NNS
cord-271701-tx0lqgff	133	19	formed	form	VBN
cord-271701-tx0lqgff	133	20	by	by	IN
cord-271701-tx0lqgff	133	21	both	both	CC
cord-271701-tx0lqgff	133	22	nsp8	nsp8	NN
cord-271701-tx0lqgff	133	23	and	and	CC
cord-271701-tx0lqgff	133	24	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	133	25	was	be	VBD
cord-271701-tx0lqgff	133	26	$	$	$
cord-271701-tx0lqgff	133	27	2-fold	2-fold	CD
cord-271701-tx0lqgff	133	28	higher	high	JJR
cord-271701-tx0lqgff	133	29	than	than	IN
cord-271701-tx0lqgff	133	30	for	for	IN
cord-271701-tx0lqgff	133	31	complexes	complex	NNS
cord-271701-tx0lqgff	133	32	formed	form	VBN
cord-271701-tx0lqgff	133	33	by	by	IN
cord-271701-tx0lqgff	133	34	His	-PRON-	PRP$
cord-271701-tx0lqgff	133	35	-	-	HYPH
cord-271701-tx0lqgff	133	36	nsp8	nsp8	NN
cord-271701-tx0lqgff	133	37	.	.	.
cord-271701-tx0lqgff	134	1	(	(	-LRB-
cord-271701-tx0lqgff	134	2	F	F	NNP
cord-271701-tx0lqgff	134	3	)	)	-RRB-
cord-271701-tx0lqgff	134	4	Comparative	comparative	JJ
cord-271701-tx0lqgff	134	5	analysis	analysis	NN
cord-271701-tx0lqgff	134	6	of	of	IN
cord-271701-tx0lqgff	134	7	nsp8	nsp8	NN
cord-271701-tx0lqgff	134	8	,	,	,
cord-271701-tx0lqgff	134	9	nsp(7	nsp(7	IN
cord-271701-tx0lqgff	134	10	+	+	SYM
cord-271701-tx0lqgff	134	11	8	8	CD
cord-271701-tx0lqgff	134	12	)	)	-RRB-
cord-271701-tx0lqgff	134	13	,	,	,
cord-271701-tx0lqgff	134	14	His	-PRON-	PRP$
cord-271701-tx0lqgff	134	15	-	-	HYPH
cord-271701-tx0lqgff	134	16	nsp8	nsp8	NN
cord-271701-tx0lqgff	134	17	and	and	CC
cord-271701-tx0lqgff	134	18	nsp7+nsp8-His	nsp7+nsp8-his	NN
cord-271701-tx0lqgff	134	19	.	.	.
cord-271701-tx0lqgff	135	1	Only	only	RB
cord-271701-tx0lqgff	135	2	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	135	3	+	+	SYM
cord-271701-tx0lqgff	135	4	8	8	CD
cord-271701-tx0lqgff	135	5	)	)	-RRB-
cord-271701-tx0lqgff	135	6	showed	show	VBD
cord-271701-tx0lqgff	135	7	a	a	DT
cord-271701-tx0lqgff	135	8	molecular	molecular	JJ
cord-271701-tx0lqgff	135	9	weight	weight	NN
cord-271701-tx0lqgff	135	10	shift	shift	NN
cord-271701-tx0lqgff	135	11	to	to	IN
cord-271701-tx0lqgff	135	12	the	the	DT
cord-271701-tx0lqgff	135	13	$	$	$
cord-271701-tx0lqgff	135	14	225-kDa	225-kda	CD
cord-271701-tx0lqgff	135	15	size	size	NN
cord-271701-tx0lqgff	135	16	range	range	NN
cord-271701-tx0lqgff	135	17	with	with	IN
cord-271701-tx0lqgff	135	18	a	a	DT
cord-271701-tx0lqgff	135	19	standard	standard	JJ
cord-271701-tx0lqgff	135	20	deviation	deviation	NN
cord-271701-tx0lqgff	135	21	of	of	IN
cord-271701-tx0lqgff	135	22	15-kDa	15-kda	CD
cord-271701-tx0lqgff	135	23	(	(	-LRB-
cord-271701-tx0lqgff	135	24	n	n	NN
cord-271701-tx0lqgff	135	25	=	=	SYM
cord-271701-tx0lqgff	135	26	3	3	CD
cord-271701-tx0lqgff	135	27	)	)	-RRB-
cord-271701-tx0lqgff	135	28	.	.	.
cord-271701-tx0lqgff	136	1	This	this	DT
cord-271701-tx0lqgff	136	2	size	size	NN
cord-271701-tx0lqgff	136	3	is	be	VBZ
cord-271701-tx0lqgff	136	4	indicative	indicative	JJ
cord-271701-tx0lqgff	136	5	of	of	IN
cord-271701-tx0lqgff	136	6	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	136	7	formation	formation	NN
cord-271701-tx0lqgff	136	8	,	,	,
cord-271701-tx0lqgff	136	9	whereas	whereas	IN
cord-271701-tx0lqgff	136	10	the	the	DT
cord-271701-tx0lqgff	136	11	analysis	analysis	NN
cord-271701-tx0lqgff	136	12	of	of	IN
cord-271701-tx0lqgff	136	13	nsp7+nsp8-His	nsp7+nsp8-his	NN
cord-271701-tx0lqgff	136	14	showed	show	VBD
cord-271701-tx0lqgff	136	15	dominant	dominant	JJ
cord-271701-tx0lqgff	136	16	peaks	peak	NNS
cord-271701-tx0lqgff	136	17	of	of	IN
cord-271701-tx0lqgff	136	18	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	136	19	and	and	CC
cord-271701-tx0lqgff	136	20	nsp7	nsp7	NN
cord-271701-tx0lqgff	136	21	(	(	-LRB-
cord-271701-tx0lqgff	136	22	which	which	WDT
cord-271701-tx0lqgff	136	23	is	be	VBZ
cord-271701-tx0lqgff	136	24	$	$	$
cord-271701-tx0lqgff	136	25	10	10	CD
cord-271701-tx0lqgff	136	26	kDa	kDa	NNS
cord-271701-tx0lqgff	136	27	as	as	IN
cord-271701-tx0lqgff	136	28	a	a	DT
cord-271701-tx0lqgff	136	29	monomer	monomer	NN
cord-271701-tx0lqgff	136	30	)	)	-RRB-
cord-271701-tx0lqgff	136	31	.	.	.
cord-271701-tx0lqgff	137	1	octamer	octamer	NNP
cord-271701-tx0lqgff	137	2	by	by	IN
cord-271701-tx0lqgff	137	3	eight	eight	CD
cord-271701-tx0lqgff	137	4	copies	copy	NNS
cord-271701-tx0lqgff	137	5	of	of	IN
cord-271701-tx0lqgff	137	6	nsp7	nsp7	NNP
cord-271701-tx0lqgff	137	7	thus	thus	RB
cord-271701-tx0lqgff	137	8	appears	appear	VBZ
cord-271701-tx0lqgff	137	9	to	to	TO
cord-271701-tx0lqgff	137	10	be	be	VB
cord-271701-tx0lqgff	137	11	redundant	redundant	JJ
cord-271701-tx0lqgff	137	12	,	,	,
cord-271701-tx0lqgff	137	13	in	in	IN
cord-271701-tx0lqgff	137	14	line	line	NN
cord-271701-tx0lqgff	137	15	with	with	IN
cord-271701-tx0lqgff	137	16	the	the	DT
cord-271701-tx0lqgff	137	17	critical	critical	JJ
cord-271701-tx0lqgff	137	18	role	role	NN
cord-271701-tx0lqgff	137	19	for	for	IN
cord-271701-tx0lqgff	137	20	the	the	DT
cord-271701-tx0lqgff	137	21	nsp8	nsp8	NNP
cord-271701-tx0lqgff	137	22	N	n	CD
cord-271701-tx0lqgff	137	23	-	-	HYPH
cord-271701-tx0lqgff	137	24	terminal	terminal	NN
cord-271701-tx0lqgff	137	25	domain	domain	NN
cord-271701-tx0lqgff	137	26	described	describe	VBN
cord-271701-tx0lqgff	137	27	above	above	RB
cord-271701-tx0lqgff	137	28	.	.	.
cord-271701-tx0lqgff	138	1	We	-PRON-	PRP
cord-271701-tx0lqgff	138	2	surmised	surmise	VBD
cord-271701-tx0lqgff	138	3	therefore	therefore	RB
cord-271701-tx0lqgff	138	4	that	that	IN
cord-271701-tx0lqgff	138	5	the	the	DT
cord-271701-tx0lqgff	138	6	additional	additional	JJ
cord-271701-tx0lqgff	138	7	complexity	complexity	NN
cord-271701-tx0lqgff	138	8	must	must	MD
cord-271701-tx0lqgff	138	9	have	have	VB
cord-271701-tx0lqgff	138	10	evolved	evolve	VBN
cord-271701-tx0lqgff	138	11	to	to	TO
cord-271701-tx0lqgff	138	12	improve	improve	VB
cord-271701-tx0lqgff	138	13	nsp8	nsp8	NNP
cord-271701-tx0lqgff	138	14	's	's	POS
cord-271701-tx0lqgff	138	15	function	function	NN
cord-271701-tx0lqgff	138	16	and	and	CC
cord-271701-tx0lqgff	138	17	set	set	VBD
cord-271701-tx0lqgff	138	18	out	out	RP
cord-271701-tx0lqgff	138	19	to	to	TO
cord-271701-tx0lqgff	138	20	compare	compare	VB
cord-271701-tx0lqgff	138	21	the	the	DT
cord-271701-tx0lqgff	138	22	RNA	RNA	NNP
cord-271701-tx0lqgff	138	23	binding	binding	NN
cord-271701-tx0lqgff	138	24	capabilities	capability	NNS
cord-271701-tx0lqgff	138	25	of	of	IN
cord-271701-tx0lqgff	138	26	the	the	DT
cord-271701-tx0lqgff	138	27	purified	purify	VBN
cord-271701-tx0lqgff	138	28	nsp8	nsp8	NNP
cord-271701-tx0lqgff	138	29	octamer	octamer	NN
cord-271701-tx0lqgff	138	30	and	and	CC
cord-271701-tx0lqgff	138	31	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	138	32	+	+	SYM
cord-271701-tx0lqgff	138	33	8	8	CD
cord-271701-tx0lqgff	138	34	)	)	-RRB-
cord-271701-tx0lqgff	138	35	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	138	36	.	.	.
cord-271701-tx0lqgff	139	1	By	by	IN
cord-271701-tx0lqgff	139	2	analysing	analyse	VBG
cord-271701-tx0lqgff	139	3	the	the	DT
cord-271701-tx0lqgff	139	4	steady	steady	JJ
cord-271701-tx0lqgff	139	5	-	-	HYPH
cord-271701-tx0lqgff	139	6	state	state	NN
cord-271701-tx0lqgff	139	7	ribonucleotide	ribonucleotide	NN
cord-271701-tx0lqgff	139	8	-	-	HYPH
cord-271701-tx0lqgff	139	9	protein	protein	NN
cord-271701-tx0lqgff	139	10	(	(	-LRB-
cord-271701-tx0lqgff	139	11	RNP	RNP	NNP
cord-271701-tx0lqgff	139	12	)	)	-RRB-
cord-271701-tx0lqgff	139	13	complexes	complex	NNS
cord-271701-tx0lqgff	139	14	formed	form	VBN
cord-271701-tx0lqgff	139	15	through	through	IN
cord-271701-tx0lqgff	139	16	binding	binding	NN
cord-271701-tx0lqgff	139	17	of	of	IN
cord-271701-tx0lqgff	139	18	nsp8	nsp8	NN
cord-271701-tx0lqgff	139	19	to	to	IN
cord-271701-tx0lqgff	139	20	5	5	CD
cord-271701-tx0lqgff	139	21	0	0	CD
cord-271701-tx0lqgff	139	22	32	32	CD
cord-271701-tx0lqgff	139	23	P	p	NN
cord-271701-tx0lqgff	139	24	-	-	HYPH
cord-271701-tx0lqgff	139	25	labelled	label	VBN
cord-271701-tx0lqgff	139	26	dsRNA	dsrna	NN
cord-271701-tx0lqgff	140	1	(	(	-LRB-
cord-271701-tx0lqgff	140	2	Figure	figure	NN
cord-271701-tx0lqgff	140	3	3A	3a	CD
cord-271701-tx0lqgff	140	4	)	)	-RRB-
cord-271701-tx0lqgff	141	1	,	,	,
cord-271701-tx0lqgff	141	2	we	-PRON-	PRP
cord-271701-tx0lqgff	141	3	estimated	estimate	VBD
cord-271701-tx0lqgff	141	4	the	the	DT
cord-271701-tx0lqgff	141	5	nsp8	nsp8	NNP
cord-271701-tx0lqgff	141	6	dissociation	dissociation	NN
cord-271701-tx0lqgff	141	7	constant	constant	NN
cord-271701-tx0lqgff	141	8	(	(	-LRB-
cord-271701-tx0lqgff	141	9	K	K	NNP
cord-271701-tx0lqgff	141	10	d	d	NNP
cord-271701-tx0lqgff	141	11	)	)	-RRB-
cord-271701-tx0lqgff	141	12	for	for	IN
cord-271701-tx0lqgff	141	13	dsRNA	dsrna	NN
cord-271701-tx0lqgff	142	1	to	to	TO
cord-271701-tx0lqgff	142	2	be	be	VB
cord-271701-tx0lqgff	142	3	$	$	$
cord-271701-tx0lqgff	142	4	3.3	3.3	CD
cord-271701-tx0lqgff	142	5	mM	mm	CD
cord-271701-tx0lqgff	142	6	(	(	-LRB-
cord-271701-tx0lqgff	142	7	Figure	Figure	NNP
cord-271701-tx0lqgff	142	8	3F	3F	NNP
cord-271701-tx0lqgff	142	9	)	)	-RRB-
cord-271701-tx0lqgff	142	10	,	,	,
cord-271701-tx0lqgff	142	11	which	which	WDT
cord-271701-tx0lqgff	142	12	is	be	VBZ
cord-271701-tx0lqgff	142	13	about	about	RB
cord-271701-tx0lqgff	142	14	$	$	$
cord-271701-tx0lqgff	142	15	25-fold	25-fold	CD
cord-271701-tx0lqgff	142	16	higher	high	JJR
cord-271701-tx0lqgff	142	17	than	than	IN
cord-271701-tx0lqgff	142	18	the	the	DT
cord-271701-tx0lqgff	142	19	apparent	apparent	JJ
cord-271701-tx0lqgff	142	20	K	K	NNP
cord-271701-tx0lqgff	142	21	d	d	NN
cord-271701-tx0lqgff	142	22	of	of	IN
cord-271701-tx0lqgff	142	23	nsp12	nsp12	CD
cord-271701-tx0lqgff	142	24	under	under	IN
cord-271701-tx0lqgff	142	25	comparable	comparable	JJ
cord-271701-tx0lqgff	142	26	conditions	condition	NNS
cord-271701-tx0lqgff	142	27	(	(	-LRB-
cord-271701-tx0lqgff	142	28	10	10	CD
cord-271701-tx0lqgff	142	29	)	)	-RRB-
cord-271701-tx0lqgff	142	30	.	.	.
cord-271701-tx0lqgff	143	1	A	a	DT
cord-271701-tx0lqgff	143	2	comprehensive	comprehensive	JJ
cord-271701-tx0lqgff	143	3	analysis	analysis	NN
cord-271701-tx0lqgff	143	4	of	of	IN
cord-271701-tx0lqgff	143	5	the	the	DT
cord-271701-tx0lqgff	143	6	influence	influence	NN
cord-271701-tx0lqgff	143	7	of	of	IN
cord-271701-tx0lqgff	143	8	nsp7	nsp7	NN
cord-271701-tx0lqgff	143	9	on	on	IN
cord-271701-tx0lqgff	143	10	nsp8-dependent	nsp8-dependent	NNP
cord-271701-tx0lqgff	143	11	RNA	RNA	NNP
cord-271701-tx0lqgff	143	12	binding	binding	NN
cord-271701-tx0lqgff	143	13	required	require	VBD
cord-271701-tx0lqgff	143	14	an	an	DT
cord-271701-tx0lqgff	143	15	nsp8	nsp8	JJ
cord-271701-tx0lqgff	143	16	mutant	mutant	NN
cord-271701-tx0lqgff	143	17	that	that	WDT
cord-271701-tx0lqgff	143	18	was	be	VBD
cord-271701-tx0lqgff	143	19	incapable	incapable	JJ
cord-271701-tx0lqgff	143	20	of	of	IN
cord-271701-tx0lqgff	143	21	RNA	RNA	NNP
cord-271701-tx0lqgff	143	22	binding	binding	NN
cord-271701-tx0lqgff	143	23	.	.	.
cord-271701-tx0lqgff	144	1	To	to	IN
cord-271701-tx0lqgff	144	2	this	this	DT
cord-271701-tx0lqgff	144	3	end	end	NN
cord-271701-tx0lqgff	144	4	,	,	,
cord-271701-tx0lqgff	144	5	we	-PRON-	PRP
cord-271701-tx0lqgff	144	6	engineered	engineer	VBD
cord-271701-tx0lqgff	144	7	an	an	DT
cord-271701-tx0lqgff	144	8	alanine	alanine	JJ
cord-271701-tx0lqgff	144	9	substitution	substitution	NN
cord-271701-tx0lqgff	144	10	of	of	IN
cord-271701-tx0lqgff	144	11	the	the	DT
cord-271701-tx0lqgff	144	12	conserved	conserved	JJ
cord-271701-tx0lqgff	144	13	residue	residue	NN
cord-271701-tx0lqgff	144	14	K58	K58	NNP
cord-271701-tx0lqgff	144	15	,	,	,
cord-271701-tx0lqgff	144	16	which	which	WDT
cord-271701-tx0lqgff	144	17	resides	reside	VBZ
cord-271701-tx0lqgff	144	18	in	in	IN
cord-271701-tx0lqgff	144	19	nsp8	nsp8	NNP
cord-271701-tx0lqgff	144	20	's	's	POS
cord-271701-tx0lqgff	144	21	proposed	propose	VBN
cord-271701-tx0lqgff	144	22	dsRNA	dsrna	RB
cord-271701-tx0lqgff	144	23	-	-	HYPH
cord-271701-tx0lqgff	144	24	binding	bind	VBG
cord-271701-tx0lqgff	144	25	channel	channel	NN
cord-271701-tx0lqgff	144	26	[	[	-LRB-
cord-271701-tx0lqgff	144	27	residues	residue	NNS
cord-271701-tx0lqgff	144	28	55	55	CD
cord-271701-tx0lqgff	144	29	-	-	HYPH
cord-271701-tx0lqgff	144	30	78	78	CD
cord-271701-tx0lqgff	144	31	(	(	-LRB-
cord-271701-tx0lqgff	144	32	13	13	CD
cord-271701-tx0lqgff	144	33	)	)	-RRB-
cord-271701-tx0lqgff	144	34	]	]	-RRB-
cord-271701-tx0lqgff	144	35	.	.	.
cord-271701-tx0lqgff	145	1	As	as	IN
cord-271701-tx0lqgff	145	2	is	be	VBZ
cord-271701-tx0lqgff	145	3	evident	evident	JJ
cord-271701-tx0lqgff	145	4	from	from	IN
cord-271701-tx0lqgff	145	5	the	the	DT
cord-271701-tx0lqgff	145	6	electromobility	electromobility	NN
cord-271701-tx0lqgff	145	7	shift	shift	NN
cord-271701-tx0lqgff	145	8	assay	assay	NN
cord-271701-tx0lqgff	145	9	in	in	IN
cord-271701-tx0lqgff	145	10	Figure	Figure	NNP
cord-271701-tx0lqgff	145	11	3B	3B	NNP
cord-271701-tx0lqgff	145	12	,	,	,
cord-271701-tx0lqgff	145	13	this	this	DT
cord-271701-tx0lqgff	145	14	mutation	mutation	NN
cord-271701-tx0lqgff	145	15	was	be	VBD
cord-271701-tx0lqgff	145	16	sufficient	sufficient	JJ
cord-271701-tx0lqgff	145	17	to	to	TO
cord-271701-tx0lqgff	145	18	significantly	significantly	RB
cord-271701-tx0lqgff	145	19	disrupt	disrupt	VB
cord-271701-tx0lqgff	145	20	RNA	RNA	NNP
cord-271701-tx0lqgff	145	21	binding	binding	NN
cord-271701-tx0lqgff	145	22	.	.	.
cord-271701-tx0lqgff	146	1	As	as	IN
cord-271701-tx0lqgff	146	2	a	a	DT
cord-271701-tx0lqgff	146	3	control	control	NN
cord-271701-tx0lqgff	146	4	,	,	,
cord-271701-tx0lqgff	146	5	we	-PRON-	PRP
cord-271701-tx0lqgff	146	6	also	also	RB
cord-271701-tx0lqgff	146	7	performed	perform	VBD
cord-271701-tx0lqgff	146	8	an	an	DT
cord-271701-tx0lqgff	146	9	aspartate	aspartate	NN
cord-271701-tx0lqgff	146	10	-	-	HYPH
cord-271701-tx0lqgff	146	11	to	to	IN
cord-271701-tx0lqgff	146	12	-	-	HYPH
cord-271701-tx0lqgff	146	13	alanine	alanine	NN
cord-271701-tx0lqgff	146	14	substitution	substitution	NN
cord-271701-tx0lqgff	146	15	at	at	IN
cord-271701-tx0lqgff	146	16	position	position	NN
cord-271701-tx0lqgff	146	17	52	52	CD
cord-271701-tx0lqgff	146	18	,	,	,
cord-271701-tx0lqgff	146	19	which	which	WDT
cord-271701-tx0lqgff	146	20	is	be	VBZ
cord-271701-tx0lqgff	146	21	partially	partially	RB
cord-271701-tx0lqgff	146	22	conserved	conserve	VBN
cord-271701-tx0lqgff	146	23	,	,	,
cord-271701-tx0lqgff	146	24	yet	yet	CC
cord-271701-tx0lqgff	146	25	not	not	RB
cord-271701-tx0lqgff	146	26	expected	expect	VBN
cord-271701-tx0lqgff	146	27	to	to	TO
cord-271701-tx0lqgff	146	28	participate	participate	VB
cord-271701-tx0lqgff	146	29	in	in	IN
cord-271701-tx0lqgff	146	30	RNA	RNA	NNP
cord-271701-tx0lqgff	146	31	backbone	backbone	NN
cord-271701-tx0lqgff	146	32	binding	bind	VBG
cord-271701-tx0lqgff	146	33	due	due	IN
cord-271701-tx0lqgff	146	34	to	to	IN
cord-271701-tx0lqgff	146	35	its	-PRON-	PRP$
cord-271701-tx0lqgff	146	36	negative	negative	JJ
cord-271701-tx0lqgff	146	37	charge	charge	NN
cord-271701-tx0lqgff	146	38	and	and	CC
cord-271701-tx0lqgff	146	39	position	position	NN
cord-271701-tx0lqgff	146	40	just	just	RB
cord-271701-tx0lqgff	146	41	outside	outside	IN
cord-271701-tx0lqgff	146	42	the	the	DT
cord-271701-tx0lqgff	146	43	proposed	propose	VBN
cord-271701-tx0lqgff	146	44	RNA	RNA	NNP
cord-271701-tx0lqgff	146	45	binding	binding	NN
cord-271701-tx0lqgff	146	46	channel	channel	NN
cord-271701-tx0lqgff	146	47	.	.	.
cord-271701-tx0lqgff	147	1	Indeed	indeed	RB
cord-271701-tx0lqgff	147	2	,	,	,
cord-271701-tx0lqgff	147	3	the	the	DT
cord-271701-tx0lqgff	147	4	D52A	d52a	JJ
cord-271701-tx0lqgff	147	5	mutation	mutation	NN
cord-271701-tx0lqgff	147	6	only	only	RB
cord-271701-tx0lqgff	147	7	induced	induce	VBD
cord-271701-tx0lqgff	147	8	a	a	DT
cord-271701-tx0lqgff	147	9	migratory	migratory	JJ
cord-271701-tx0lqgff	147	10	shift	shift	NN
cord-271701-tx0lqgff	147	11	of	of	IN
cord-271701-tx0lqgff	147	12	the	the	DT
cord-271701-tx0lqgff	147	13	dominant	dominant	JJ
cord-271701-tx0lqgff	147	14	RNP	RNP	NNP
cord-271701-tx0lqgff	147	15	signal	signal	NN
cord-271701-tx0lqgff	147	16	towards	towards	IN
cord-271701-tx0lqgff	147	17	the	the	DT
cord-271701-tx0lqgff	147	18	anode	anode	NN
cord-271701-tx0lqgff	147	19	,	,	,
cord-271701-tx0lqgff	147	20	likely	likely	JJ
cord-271701-tx0lqgff	147	21	as	as	IN
cord-271701-tx0lqgff	147	22	a	a	DT
cord-271701-tx0lqgff	147	23	result	result	NN
cord-271701-tx0lqgff	147	24	of	of	IN
cord-271701-tx0lqgff	147	25	the	the	DT
cord-271701-tx0lqgff	147	26	lost	lose	VBN
cord-271701-tx0lqgff	147	27	negative	negative	JJ
cord-271701-tx0lqgff	147	28	charge	charge	NN
cord-271701-tx0lqgff	147	29	(	(	-LRB-
cord-271701-tx0lqgff	147	30	Figure	figure	NN
cord-271701-tx0lqgff	147	31	3C	3c	NN
cord-271701-tx0lqgff	147	32	)	)	-RRB-
cord-271701-tx0lqgff	147	33	.	.	.
cord-271701-tx0lqgff	148	1	With	with	IN
cord-271701-tx0lqgff	148	2	the	the	DT
cord-271701-tx0lqgff	148	3	results	result	NNS
cord-271701-tx0lqgff	148	4	obtained	obtain	VBN
cord-271701-tx0lqgff	148	5	with	with	IN
cord-271701-tx0lqgff	148	6	these	these	DT
cord-271701-tx0lqgff	148	7	control	control	NN
cord-271701-tx0lqgff	148	8	proteins	protein	NNS
cord-271701-tx0lqgff	148	9	in	in	IN
cord-271701-tx0lqgff	148	10	mind	mind	NN
cord-271701-tx0lqgff	148	11	,	,	,
cord-271701-tx0lqgff	148	12	we	-PRON-	PRP
cord-271701-tx0lqgff	148	13	next	next	RB
cord-271701-tx0lqgff	148	14	explored	explore	VBD
cord-271701-tx0lqgff	148	15	the	the	DT
cord-271701-tx0lqgff	148	16	contribution	contribution	NN
cord-271701-tx0lqgff	148	17	of	of	IN
cord-271701-tx0lqgff	148	18	nsp7	nsp7	NN
cord-271701-tx0lqgff	148	19	to	to	IN
cord-271701-tx0lqgff	148	20	RNA	RNA	NNP
cord-271701-tx0lqgff	148	21	binding	bind	VBG
cord-271701-tx0lqgff	148	22	by	by	IN
cord-271701-tx0lqgff	148	23	the	the	DT
cord-271701-tx0lqgff	148	24	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	148	25	+	+	SYM
cord-271701-tx0lqgff	148	26	8	8	CD
cord-271701-tx0lqgff	148	27	)	)	-RRB-
cord-271701-tx0lqgff	148	28	complex	complex	NN
cord-271701-tx0lqgff	148	29	.	.	.
cord-271701-tx0lqgff	149	1	We	-PRON-	PRP
cord-271701-tx0lqgff	149	2	used	use	VBD
cord-271701-tx0lqgff	149	3	a	a	DT
cord-271701-tx0lqgff	149	4	fixed	fix	VBN
cord-271701-tx0lqgff	149	5	concentration	concentration	NN
cord-271701-tx0lqgff	149	6	of	of	IN
cord-271701-tx0lqgff	149	7	nsp7	nsp7	NN
cord-271701-tx0lqgff	149	8	and	and	CC
cord-271701-tx0lqgff	149	9	added	add	VBD
cord-271701-tx0lqgff	149	10	either	either	CC
cord-271701-tx0lqgff	149	11	wild	wild	JJ
cord-271701-tx0lqgff	149	12	-	-	HYPH
cord-271701-tx0lqgff	149	13	type	type	NN
cord-271701-tx0lqgff	149	14	or	or	CC
cord-271701-tx0lqgff	149	15	mutant	mutant	JJ
cord-271701-tx0lqgff	149	16	nsp8	nsp8	NN
cord-271701-tx0lqgff	149	17	up	up	IN
cord-271701-tx0lqgff	149	18	to	to	IN
cord-271701-tx0lqgff	149	19	the	the	DT
cord-271701-tx0lqgff	149	20	point	point	NN
cord-271701-tx0lqgff	149	21	where	where	WRB
cord-271701-tx0lqgff	149	22	the	the	DT
cord-271701-tx0lqgff	149	23	nsp7	nsp7	JJ
cord-271701-tx0lqgff	149	24	:	:	:
cord-271701-tx0lqgff	149	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	149	26	ratio	ratio	NN
cord-271701-tx0lqgff	149	27	reached	reach	VBD
cord-271701-tx0lqgff	149	28	equimolarity	equimolarity	NN
cord-271701-tx0lqgff	149	29	.	.	.
cord-271701-tx0lqgff	150	1	No	no	DT
cord-271701-tx0lqgff	150	2	RNA	RNA	NNP
cord-271701-tx0lqgff	150	3	binding	binding	NN
cord-271701-tx0lqgff	150	4	was	be	VBD
cord-271701-tx0lqgff	150	5	observed	observe	VBN
cord-271701-tx0lqgff	150	6	in	in	IN
cord-271701-tx0lqgff	150	7	the	the	DT
cord-271701-tx0lqgff	150	8	absence	absence	NN
cord-271701-tx0lqgff	150	9	of	of	IN
cord-271701-tx0lqgff	150	10	nsp8	nsp8	NN
cord-271701-tx0lqgff	150	11	,	,	,
cord-271701-tx0lqgff	150	12	but	but	CC
cord-271701-tx0lqgff	150	13	upon	upon	IN
cord-271701-tx0lqgff	150	14	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	150	15	+	+	SYM
cord-271701-tx0lqgff	150	16	8	8	CD
cord-271701-tx0lqgff	150	17	)	)	-RRB-
cord-271701-tx0lqgff	150	18	complex	complex	JJ
cord-271701-tx0lqgff	150	19	formation	formation	NN
cord-271701-tx0lqgff	150	20	the	the	DT
cord-271701-tx0lqgff	150	21	amount	amount	NN
cord-271701-tx0lqgff	150	22	of	of	IN
cord-271701-tx0lqgff	150	23	bound	bind	VBN
cord-271701-tx0lqgff	150	24	dsRNA	dsrna	NN
cord-271701-tx0lqgff	150	25	rapidly	rapidly	RB
cord-271701-tx0lqgff	150	26	increased	increase	VBN
cord-271701-tx0lqgff	150	27	(	(	-LRB-
cord-271701-tx0lqgff	150	28	Figure	Figure	NNP
cord-271701-tx0lqgff	150	29	3D	3D	NNP
cord-271701-tx0lqgff	150	30	)	)	-RRB-
cord-271701-tx0lqgff	150	31	.	.	.
cord-271701-tx0lqgff	151	1	Indicative	indicative	JJ
cord-271701-tx0lqgff	151	2	of	of	IN
cord-271701-tx0lqgff	151	3	successful	successful	JJ
cord-271701-tx0lqgff	151	4	complex	complex	JJ
cord-271701-tx0lqgff	151	5	formation	formation	NN
cord-271701-tx0lqgff	151	6	,	,	,
cord-271701-tx0lqgff	151	7	we	-PRON-	PRP
cord-271701-tx0lqgff	151	8	also	also	RB
cord-271701-tx0lqgff	151	9	observed	observe	VBD
cord-271701-tx0lqgff	151	10	a	a	DT
cord-271701-tx0lqgff	151	11	shift	shift	NN
cord-271701-tx0lqgff	151	12	in	in	IN
cord-271701-tx0lqgff	151	13	the	the	DT
cord-271701-tx0lqgff	151	14	molecular	molecular	JJ
cord-271701-tx0lqgff	151	15	weight	weight	NN
cord-271701-tx0lqgff	151	16	of	of	IN
cord-271701-tx0lqgff	151	17	the	the	DT
cord-271701-tx0lqgff	151	18	major	major	JJ
cord-271701-tx0lqgff	151	19	RNP	RNP	NNP
cord-271701-tx0lqgff	151	20	complex	complex	NN
cord-271701-tx0lqgff	151	21	formed	form	VBN
cord-271701-tx0lqgff	151	22	(	(	-LRB-
cord-271701-tx0lqgff	151	23	Figure	Figure	NNP
cord-271701-tx0lqgff	151	24	3D	3D	NNP
cord-271701-tx0lqgff	151	25	)	)	-RRB-
cord-271701-tx0lqgff	151	26	.	.	.
cord-271701-tx0lqgff	152	1	Western	western	JJ
cord-271701-tx0lqgff	152	2	blot	blot	NN
cord-271701-tx0lqgff	152	3	analysis	analysis	NN
cord-271701-tx0lqgff	152	4	confirmed	confirm	VBD
cord-271701-tx0lqgff	152	5	that	that	IN
cord-271701-tx0lqgff	152	6	both	both	CC
cord-271701-tx0lqgff	152	7	nsp7	nsp7	NN
cord-271701-tx0lqgff	152	8	and	and	CC
cord-271701-tx0lqgff	152	9	nsp8	nsp8	NN
cord-271701-tx0lqgff	152	10	were	be	VBD
cord-271701-tx0lqgff	152	11	present	present	JJ
cord-271701-tx0lqgff	152	12	at	at	IN
cord-271701-tx0lqgff	152	13	this	this	DT
cord-271701-tx0lqgff	152	14	position	position	NN
cord-271701-tx0lqgff	152	15	in	in	IN
cord-271701-tx0lqgff	152	16	the	the	DT
cord-271701-tx0lqgff	152	17	gel	gel	NN
cord-271701-tx0lqgff	152	18	(	(	-LRB-
cord-271701-tx0lqgff	152	19	not	not	RB
cord-271701-tx0lqgff	152	20	shown	show	VBN
cord-271701-tx0lqgff	152	21	)	)	-RRB-
cord-271701-tx0lqgff	152	22	,	,	,
cord-271701-tx0lqgff	152	23	but	but	CC
cord-271701-tx0lqgff	152	24	due	due	IN
cord-271701-tx0lqgff	152	25	to	to	IN
cord-271701-tx0lqgff	152	26	the	the	DT
cord-271701-tx0lqgff	152	27	generally	generally	RB
cord-271701-tx0lqgff	152	28	unpredictable	unpredictable	JJ
cord-271701-tx0lqgff	152	29	migration	migration	NN
cord-271701-tx0lqgff	152	30	behaviour	behaviour	NN
cord-271701-tx0lqgff	152	31	of	of	IN
cord-271701-tx0lqgff	152	32	proteins	protein	NNS
cord-271701-tx0lqgff	152	33	and	and	CC
cord-271701-tx0lqgff	152	34	RNPs	rnp	NNS
cord-271701-tx0lqgff	152	35	in	in	IN
cord-271701-tx0lqgff	152	36	native	native	JJ
cord-271701-tx0lqgff	152	37	PAGE	page	NN
cord-271701-tx0lqgff	152	38	,	,	,
cord-271701-tx0lqgff	152	39	it	-PRON-	PRP
cord-271701-tx0lqgff	152	40	was	be	VBD
cord-271701-tx0lqgff	152	41	not	not	RB
cord-271701-tx0lqgff	152	42	possible	possible	JJ
cord-271701-tx0lqgff	152	43	to	to	TO
cord-271701-tx0lqgff	152	44	assess	assess	VB
cord-271701-tx0lqgff	152	45	whether	whether	IN
cord-271701-tx0lqgff	152	46	this	this	DT
cord-271701-tx0lqgff	152	47	band	band	NN
cord-271701-tx0lqgff	152	48	indeed	indeed	RB
cord-271701-tx0lqgff	152	49	corresponded	correspond	VBD
cord-271701-tx0lqgff	152	50	to	to	IN
cord-271701-tx0lqgff	152	51	the	the	DT
cord-271701-tx0lqgff	152	52	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	152	53	+	+	SYM
cord-271701-tx0lqgff	152	54	8	8	CD
cord-271701-tx0lqgff	152	55	)	)	-RRB-
cord-271701-tx0lqgff	152	56	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	152	57	.	.	.
cord-271701-tx0lqgff	153	1	The	the	DT
cord-271701-tx0lqgff	153	2	K	K	NNP
cord-271701-tx0lqgff	153	3	d	d	NN
cord-271701-tx0lqgff	153	4	of	of	IN
cord-271701-tx0lqgff	153	5	the	the	DT
cord-271701-tx0lqgff	153	6	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	153	7	+	+	SYM
cord-271701-tx0lqgff	153	8	8	8	CD
cord-271701-tx0lqgff	153	9	)	)	-RRB-
cord-271701-tx0lqgff	153	10	complex	complex	NN
cord-271701-tx0lqgff	153	11	was	be	VBD
cord-271701-tx0lqgff	153	12	estimated	estimate	VBN
cord-271701-tx0lqgff	153	13	at	at	IN
cord-271701-tx0lqgff	153	14	$	$	$
cord-271701-tx0lqgff	153	15	1.2	1.2	CD
cord-271701-tx0lqgff	153	16	mM	mm	CD
cord-271701-tx0lqgff	153	17	,	,	,
cord-271701-tx0lqgff	153	18	about	about	RB
cord-271701-tx0lqgff	153	19	3-fold	3-fold	CD
cord-271701-tx0lqgff	153	20	lower	low	JJR
cord-271701-tx0lqgff	153	21	than	than	IN
cord-271701-tx0lqgff	153	22	that	that	DT
cord-271701-tx0lqgff	153	23	of	of	IN
cord-271701-tx0lqgff	153	24	nsp8	nsp8	NN
cord-271701-tx0lqgff	153	25	alone	alone	JJ
cord-271701-tx0lqgff	154	1	(	(	-LRB-
cord-271701-tx0lqgff	154	2	Figure	Figure	NNP
cord-271701-tx0lqgff	154	3	3F	3F	NNP
cord-271701-tx0lqgff	154	4	)	)	-RRB-
cord-271701-tx0lqgff	154	5	.	.	.
cord-271701-tx0lqgff	155	1	When	when	WRB
cord-271701-tx0lqgff	155	2	we	-PRON-	PRP
cord-271701-tx0lqgff	155	3	next	next	RB
cord-271701-tx0lqgff	155	4	added	add	VBD
cord-271701-tx0lqgff	155	5	an	an	DT
cord-271701-tx0lqgff	155	6	equimolar	equimolar	JJ
cord-271701-tx0lqgff	155	7	amount	amount	NN
cord-271701-tx0lqgff	155	8	of	of	IN
cord-271701-tx0lqgff	155	9	nsp7	nsp7	NN
cord-271701-tx0lqgff	155	10	to	to	IN
cord-271701-tx0lqgff	155	11	the	the	DT
cord-271701-tx0lqgff	155	12	nsp8	nsp8	NNP
cord-271701-tx0lqgff	155	13	RNA	RNA	NNP
cord-271701-tx0lqgff	155	14	-	-	HYPH
cord-271701-tx0lqgff	155	15	binding	bind	VBG
cord-271701-tx0lqgff	155	16	mutant	mutant	JJ
cord-271701-tx0lqgff	155	17	K58A	k58a	NN
cord-271701-tx0lqgff	155	18	,	,	,
cord-271701-tx0lqgff	155	19	we	-PRON-	PRP
cord-271701-tx0lqgff	155	20	observed	observe	VBD
cord-271701-tx0lqgff	155	21	a	a	DT
cord-271701-tx0lqgff	155	22	minor	minor	JJ
cord-271701-tx0lqgff	155	23	increase	increase	NN
cord-271701-tx0lqgff	155	24	in	in	IN
cord-271701-tx0lqgff	155	25	the	the	DT
cord-271701-tx0lqgff	155	26	binding	bind	VBG
cord-271701-tx0lqgff	155	27	affinity	affinity	NN
cord-271701-tx0lqgff	155	28	for	for	IN
cord-271701-tx0lqgff	155	29	RNA	RNA	NNP
cord-271701-tx0lqgff	155	30	(	(	-LRB-
cord-271701-tx0lqgff	155	31	compare	compare	VB
cord-271701-tx0lqgff	155	32	Figure	figure	NN
cord-271701-tx0lqgff	155	33	3B	3b	NN
cord-271701-tx0lqgff	155	34	with	with	IN
cord-271701-tx0lqgff	155	35	3E	3E	NNP
cord-271701-tx0lqgff	155	36	)	)	-RRB-
cord-271701-tx0lqgff	155	37	.	.	.
cord-271701-tx0lqgff	156	1	Mutant	mutant	JJ
cord-271701-tx0lqgff	156	2	D52A	d52a	RB
cord-271701-tx0lqgff	156	3	,	,	,
cord-271701-tx0lqgff	156	4	on	on	IN
cord-271701-tx0lqgff	156	5	the	the	DT
cord-271701-tx0lqgff	156	6	other	other	JJ
cord-271701-tx0lqgff	156	7	hand	hand	NN
cord-271701-tx0lqgff	156	8	,	,	,
cord-271701-tx0lqgff	156	9	behaved	behave	VBD
cord-271701-tx0lqgff	156	10	similar	similar	JJ
cord-271701-tx0lqgff	156	11	to	to	IN
cord-271701-tx0lqgff	156	12	the	the	DT
cord-271701-tx0lqgff	156	13	wild	wild	JJ
cord-271701-tx0lqgff	156	14	-	-	HYPH
cord-271701-tx0lqgff	156	15	type	type	NN
cord-271701-tx0lqgff	156	16	protein	protein	NN
cord-271701-tx0lqgff	156	17	(	(	-LRB-
cord-271701-tx0lqgff	156	18	Figure	Figure	NNP
cord-271701-tx0lqgff	156	19	3E	3e	NN
cord-271701-tx0lqgff	156	20	)	)	-RRB-
cord-271701-tx0lqgff	156	21	.	.	.
cord-271701-tx0lqgff	157	1	Together	together	RB
cord-271701-tx0lqgff	157	2	,	,	,
cord-271701-tx0lqgff	157	3	these	these	DT
cord-271701-tx0lqgff	157	4	results	result	NNS
cord-271701-tx0lqgff	157	5	complement	complement	VBP
cord-271701-tx0lqgff	157	6	the	the	DT
cord-271701-tx0lqgff	157	7	observation	observation	NN
cord-271701-tx0lqgff	157	8	that	that	IN
cord-271701-tx0lqgff	157	9	various	various	JJ
cord-271701-tx0lqgff	157	10	positively	positively	RB
cord-271701-tx0lqgff	157	11	charged	charge	VBN
cord-271701-tx0lqgff	157	12	nsp7	nsp7	JJ
cord-271701-tx0lqgff	157	13	residues	residue	NNS
cord-271701-tx0lqgff	157	14	line	line	VBP
cord-271701-tx0lqgff	157	15	the	the	DT
cord-271701-tx0lqgff	157	16	inside	inside	NN
cord-271701-tx0lqgff	157	17	of	of	IN
cord-271701-tx0lqgff	157	18	the	the	DT
cord-271701-tx0lqgff	157	19	nsp8-scaffolded	nsp8-scaffolded	NNP
cord-271701-tx0lqgff	157	20	RNA	RNA	NNP
cord-271701-tx0lqgff	157	21	binding	bind	VBG
cord-271701-tx0lqgff	157	22	channel	channel	NN
cord-271701-tx0lqgff	157	23	(	(	-LRB-
cord-271701-tx0lqgff	157	24	13	13	CD
cord-271701-tx0lqgff	157	25	)	)	-RRB-
cord-271701-tx0lqgff	157	26	,	,	,
cord-271701-tx0lqgff	157	27	and	and	CC
cord-271701-tx0lqgff	157	28	they	-PRON-	PRP
cord-271701-tx0lqgff	157	29	provide	provide	VBP
cord-271701-tx0lqgff	157	30	the	the	DT
cord-271701-tx0lqgff	157	31	first	first	JJ
cord-271701-tx0lqgff	157	32	direct	direct	JJ
cord-271701-tx0lqgff	157	33	evidence	evidence	NN
cord-271701-tx0lqgff	157	34	for	for	IN
cord-271701-tx0lqgff	157	35	a	a	DT
cord-271701-tx0lqgff	157	36	functional	functional	JJ
cord-271701-tx0lqgff	157	37	role	role	NN
cord-271701-tx0lqgff	157	38	of	of	IN
cord-271701-tx0lqgff	157	39	nsp7	nsp7	NN
cord-271701-tx0lqgff	157	40	in	in	IN
cord-271701-tx0lqgff	157	41	the	the	DT
cord-271701-tx0lqgff	157	42	SARS	SARS	NNP
cord-271701-tx0lqgff	157	43	-	-	HYPH
cord-271701-tx0lqgff	157	44	CoV	CoV	NNP
cord-271701-tx0lqgff	157	45	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	157	46	+	+	SYM
cord-271701-tx0lqgff	157	47	8	8	CD
cord-271701-tx0lqgff	157	48	)	)	-RRB-
cord-271701-tx0lqgff	157	49	structure	structure	NN
cord-271701-tx0lqgff	157	50	.	.	.
cord-271701-tx0lqgff	157	51	 	 	_SP
cord-271701-tx0lqgff	158	1	Given	give	VBN
cord-271701-tx0lqgff	158	2	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	158	3	+	+	SYM
cord-271701-tx0lqgff	158	4	8	8	CD
cord-271701-tx0lqgff	158	5	)	)	-RRB-
cord-271701-tx0lqgff	158	6	's	's	POS
cord-271701-tx0lqgff	158	7	ability	ability	NN
cord-271701-tx0lqgff	158	8	to	to	TO
cord-271701-tx0lqgff	158	9	bind	bind	VB
cord-271701-tx0lqgff	158	10	dsRNA	dsrna	NN
cord-271701-tx0lqgff	158	11	,	,	,
cord-271701-tx0lqgff	158	12	we	-PRON-	PRP
cord-271701-tx0lqgff	158	13	wondered	wonder	VBD
cord-271701-tx0lqgff	158	14	whether	whether	IN
cord-271701-tx0lqgff	158	15	this	this	DT
cord-271701-tx0lqgff	158	16	protein	protein	NN
cord-271701-tx0lqgff	158	17	complex	complex	NN
cord-271701-tx0lqgff	158	18	would	would	MD
cord-271701-tx0lqgff	158	19	also	also	RB
cord-271701-tx0lqgff	158	20	be	be	VB
cord-271701-tx0lqgff	158	21	catalytically	catalytically	RB
cord-271701-tx0lqgff	158	22	active	active	JJ
cord-271701-tx0lqgff	158	23	on	on	IN
cord-271701-tx0lqgff	158	24	this	this	DT
cord-271701-tx0lqgff	158	25	type	type	NN
cord-271701-tx0lqgff	158	26	of	of	IN
cord-271701-tx0lqgff	158	27	template	template	NN
cord-271701-tx0lqgff	158	28	and	and	CC
cord-271701-tx0lqgff	158	29	able	able	JJ
cord-271701-tx0lqgff	158	30	to	to	TO
cord-271701-tx0lqgff	158	31	incorporate	incorporate	VB
cord-271701-tx0lqgff	158	32	nucleoside	nucleoside	NN
cord-271701-tx0lqgff	158	33	monophosphates	monophosphate	NNS
cord-271701-tx0lqgff	158	34	(	(	-LRB-
cord-271701-tx0lqgff	158	35	NMPs	NMPs	NNP
cord-271701-tx0lqgff	158	36	)	)	-RRB-
cord-271701-tx0lqgff	158	37	into	into	IN
cord-271701-tx0lqgff	158	38	partially	partially	RB
cord-271701-tx0lqgff	158	39	double	double	RB
cord-271701-tx0lqgff	158	40	-	-	HYPH
cord-271701-tx0lqgff	158	41	stranded	strand	VBN
cord-271701-tx0lqgff	158	42	RNA	RNA	NNP
cord-271701-tx0lqgff	158	43	molecules	molecule	NNS
cord-271701-tx0lqgff	158	44	,	,	,
cord-271701-tx0lqgff	158	45	i.e.	i.e.	FW
cord-271701-tx0lqgff	158	46	primed	prime	VBN
cord-271701-tx0lqgff	158	47	templates	template	NNS
cord-271701-tx0lqgff	158	48	.	.	.
cord-271701-tx0lqgff	159	1	We	-PRON-	PRP
cord-271701-tx0lqgff	159	2	therefore	therefore	RB
cord-271701-tx0lqgff	159	3	examined	examine	VBD
cord-271701-tx0lqgff	159	4	the	the	DT
cord-271701-tx0lqgff	159	5	ability	ability	NN
cord-271701-tx0lqgff	159	6	of	of	IN
cord-271701-tx0lqgff	159	7	nsp8	nsp8	NN
cord-271701-tx0lqgff	159	8	to	to	TO
cord-271701-tx0lqgff	159	9	extend	extend	VB
cord-271701-tx0lqgff	159	10	a	a	DT
cord-271701-tx0lqgff	159	11	20-nt	20-nt	CD
cord-271701-tx0lqgff	159	12	primer	primer	NN
cord-271701-tx0lqgff	159	13	that	that	WDT
cord-271701-tx0lqgff	159	14	was	be	VBD
cord-271701-tx0lqgff	159	15	pre	pre	VBN
cord-271701-tx0lqgff	159	16	-	-	VBN
cord-271701-tx0lqgff	159	17	annealed	anneal	VBN
cord-271701-tx0lqgff	159	18	to	to	IN
cord-271701-tx0lqgff	159	19	a	a	DT
cord-271701-tx0lqgff	159	20	heteromeric	heteromeric	JJ
cord-271701-tx0lqgff	159	21	template	template	NN
cord-271701-tx0lqgff	159	22	with	with	IN
cord-271701-tx0lqgff	159	23	relatively	relatively	RB
cord-271701-tx0lqgff	159	24	low	low	JJ
cord-271701-tx0lqgff	159	25	secondary	secondary	JJ
cord-271701-tx0lqgff	159	26	structure	structure	NN
cord-271701-tx0lqgff	159	27	,	,	,
cord-271701-tx0lqgff	159	28	to	to	TO
cord-271701-tx0lqgff	159	29	rule	rule	VB
cord-271701-tx0lqgff	159	30	out	out	RP
cord-271701-tx0lqgff	159	31	potential	potential	JJ
cord-271701-tx0lqgff	159	32	adverse	adverse	JJ
cord-271701-tx0lqgff	159	33	effects	effect	NNS
cord-271701-tx0lqgff	159	34	of	of	IN
cord-271701-tx0lqgff	159	35	hairpins	hairpin	NNS
cord-271701-tx0lqgff	159	36	(	(	-LRB-
cord-271701-tx0lqgff	159	37	Figure	figure	NN
cord-271701-tx0lqgff	159	38	4A	4a	CD
cord-271701-tx0lqgff	159	39	)	)	-RRB-
cord-271701-tx0lqgff	159	40	.	.	.
cord-271701-tx0lqgff	160	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	160	2	and	and	CC
cord-271701-tx0lqgff	160	3	in	in	IN
cord-271701-tx0lqgff	160	4	contrast	contrast	NN
cord-271701-tx0lqgff	160	5	to	to	IN
cord-271701-tx0lqgff	160	6	previous	previous	JJ
cord-271701-tx0lqgff	160	7	observations	observation	NNS
cord-271701-tx0lqgff	160	8	(	(	-LRB-
cord-271701-tx0lqgff	160	9	12	12	CD
cord-271701-tx0lqgff	160	10	)	)	-RRB-
cord-271701-tx0lqgff	160	11	,	,	,
cord-271701-tx0lqgff	160	12	the	the	DT
cord-271701-tx0lqgff	160	13	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	160	14	+	+	SYM
cord-271701-tx0lqgff	160	15	8	8	CD
cord-271701-tx0lqgff	160	16	)	)	-RRB-
cord-271701-tx0lqgff	160	17	complex	complex	NN
cord-271701-tx0lqgff	160	18	readily	readily	RB
cord-271701-tx0lqgff	160	19	extended	extend	VBD
cord-271701-tx0lqgff	160	20	the	the	DT
cord-271701-tx0lqgff	160	21	primer	primer	NN
cord-271701-tx0lqgff	160	22	up	up	RP
cord-271701-tx0lqgff	160	23	to	to	IN
cord-271701-tx0lqgff	160	24	template	template	NN
cord-271701-tx0lqgff	160	25	length	length	NN
cord-271701-tx0lqgff	160	26	,	,	,
cord-271701-tx0lqgff	160	27	resulting	result	VBG
cord-271701-tx0lqgff	160	28	in	in	IN
cord-271701-tx0lqgff	160	29	the	the	DT
cord-271701-tx0lqgff	160	30	formation	formation	NN
cord-271701-tx0lqgff	160	31	of	of	IN
cord-271701-tx0lqgff	160	32	a	a	DT
cord-271701-tx0lqgff	160	33	40-base	40-base	CD
cord-271701-tx0lqgff	160	34	pair	pair	NN
cord-271701-tx0lqgff	160	35	RNA	RNA	NNP
cord-271701-tx0lqgff	160	36	duplex	duplex	NN
cord-271701-tx0lqgff	160	37	(	(	-LRB-
cord-271701-tx0lqgff	160	38	Figure	figure	NN
cord-271701-tx0lqgff	160	39	4B	4b	NN
cord-271701-tx0lqgff	160	40	)	)	-RRB-
cord-271701-tx0lqgff	160	41	.	.	.
cord-271701-tx0lqgff	161	1	The	the	DT
cord-271701-tx0lqgff	161	2	negatively	negatively	RB
cord-271701-tx0lqgff	161	3	charged	charge	VBN
cord-271701-tx0lqgff	161	4	and	and	CC
cord-271701-tx0lqgff	161	5	helical	helical	JJ
cord-271701-tx0lqgff	161	6	polymer	polymer	NN
cord-271701-tx0lqgff	161	7	heparin	heparin	NN
cord-271701-tx0lqgff	161	8	is	be	VBZ
cord-271701-tx0lqgff	161	9	able	able	JJ
cord-271701-tx0lqgff	161	10	to	to	TO
cord-271701-tx0lqgff	161	11	occupy	occupy	VB
cord-271701-tx0lqgff	161	12	the	the	DT
cord-271701-tx0lqgff	161	13	binding	bind	VBG
cord-271701-tx0lqgff	161	14	sites	site	NNS
cord-271701-tx0lqgff	161	15	of	of	IN
cord-271701-tx0lqgff	161	16	RNA	RNA	NNP
cord-271701-tx0lqgff	161	17	and	and	CC
cord-271701-tx0lqgff	161	18	DNA	DNA	NNP
cord-271701-tx0lqgff	161	19	polymerases	polymerase	NNS
cord-271701-tx0lqgff	161	20	,	,	,
cord-271701-tx0lqgff	161	21	and	and	CC
cord-271701-tx0lqgff	161	22	can	can	MD
cord-271701-tx0lqgff	161	23	thus	thus	RB
cord-271701-tx0lqgff	161	24	directly	directly	RB
cord-271701-tx0lqgff	161	25	compete	compete	VB
cord-271701-tx0lqgff	161	26	with	with	IN
cord-271701-tx0lqgff	161	27	RNA	RNA	NNP
cord-271701-tx0lqgff	161	28	and	and	CC
cord-271701-tx0lqgff	161	29	DNA	DNA	NNP
cord-271701-tx0lqgff	161	30	templates	template	NNS
cord-271701-tx0lqgff	161	31	.	.	.
cord-271701-tx0lqgff	162	1	To	to	TO
cord-271701-tx0lqgff	162	2	verify	verify	VB
cord-271701-tx0lqgff	162	3	that	that	IN
cord-271701-tx0lqgff	162	4	the	the	DT
cord-271701-tx0lqgff	162	5	full	full	JJ
cord-271701-tx0lqgff	162	6	-	-	HYPH
cord-271701-tx0lqgff	162	7	length	length	NN
cord-271701-tx0lqgff	162	8	and	and	CC
cord-271701-tx0lqgff	162	9	longer	long	JJR
cord-271701-tx0lqgff	162	10	RNA	RNA	NNP
cord-271701-tx0lqgff	162	11	products	product	NNS
cord-271701-tx0lqgff	162	12	were	be	VBD
cord-271701-tx0lqgff	162	13	derived	derive	VBN
cord-271701-tx0lqgff	162	14	from	from	IN
cord-271701-tx0lqgff	162	15	single	single	JJ
cord-271701-tx0lqgff	162	16	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	162	17	+	+	SYM
cord-271701-tx0lqgff	162	18	8	8	CD
cord-271701-tx0lqgff	162	19	)	)	-RRB-
cord-271701-tx0lqgff	162	20	complexes	complex	NNS
cord-271701-tx0lqgff	162	21	bound	bind	VBN
cord-271701-tx0lqgff	162	22	to	to	IN
cord-271701-tx0lqgff	162	23	the	the	DT
cord-271701-tx0lqgff	162	24	template	template	NN
cord-271701-tx0lqgff	162	25	(	(	-LRB-
cord-271701-tx0lqgff	162	26	i.e.	i.e.	FW
cord-271701-tx0lqgff	162	27	from	from	IN
cord-271701-tx0lqgff	162	28	a	a	DT
cord-271701-tx0lqgff	162	29	processive	processive	JJ
cord-271701-tx0lqgff	162	30	activity	activity	NN
cord-271701-tx0lqgff	162	31	)	)	-RRB-
cord-271701-tx0lqgff	162	32	,	,	,
cord-271701-tx0lqgff	162	33	and	and	CC
cord-271701-tx0lqgff	162	34	not	not	RB
cord-271701-tx0lqgff	162	35	from	from	IN
cord-271701-tx0lqgff	162	36	multiple	multiple	JJ
cord-271701-tx0lqgff	162	37	binding	binding	NN
cord-271701-tx0lqgff	162	38	and	and	CC
cord-271701-tx0lqgff	162	39	extension	extension	NN
cord-271701-tx0lqgff	162	40	events	event	NNS
cord-271701-tx0lqgff	162	41	(	(	-LRB-
cord-271701-tx0lqgff	162	42	i.e.	i.e.	FW
cord-271701-tx0lqgff	162	43	a	a	DT
cord-271701-tx0lqgff	162	44	distributive	distributive	JJ
cord-271701-tx0lqgff	162	45	activity	activity	NN
cord-271701-tx0lqgff	162	46	)	)	-RRB-
cord-271701-tx0lqgff	162	47	,	,	,
cord-271701-tx0lqgff	162	48	we	-PRON-	PRP
cord-271701-tx0lqgff	162	49	performed	perform	VBD
cord-271701-tx0lqgff	162	50	the	the	DT
cord-271701-tx0lqgff	162	51	primer	primer	NN
cord-271701-tx0lqgff	162	52	extension	extension	NN
cord-271701-tx0lqgff	162	53	reaction	reaction	NN
cord-271701-tx0lqgff	162	54	in	in	IN
cord-271701-tx0lqgff	162	55	the	the	DT
cord-271701-tx0lqgff	162	56	presence	presence	NN
cord-271701-tx0lqgff	162	57	of	of	IN
cord-271701-tx0lqgff	162	58	heparin	heparin	NN
cord-271701-tx0lqgff	162	59	to	to	TO
cord-271701-tx0lqgff	162	60	trap	trap	VB
cord-271701-tx0lqgff	162	61	any	any	DT
cord-271701-tx0lqgff	162	62	unbound	unbound	JJ
cord-271701-tx0lqgff	162	63	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	162	64	+	+	SYM
cord-271701-tx0lqgff	162	65	8	8	CD
cord-271701-tx0lqgff	162	66	)	)	-RRB-
cord-271701-tx0lqgff	162	67	.	.	.
cord-271701-tx0lqgff	163	1	We	-PRON-	PRP
cord-271701-tx0lqgff	163	2	first	first	RB
cord-271701-tx0lqgff	163	3	tested	test	VBD
cord-271701-tx0lqgff	163	4	the	the	DT
cord-271701-tx0lqgff	163	5	concentration	concentration	NN
cord-271701-tx0lqgff	163	6	required	require	VBN
cord-271701-tx0lqgff	163	7	to	to	TO
cord-271701-tx0lqgff	163	8	saturate	saturate	VB
cord-271701-tx0lqgff	163	9	all	all	DT
cord-271701-tx0lqgff	163	10	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	163	11	+	+	SYM
cord-271701-tx0lqgff	163	12	8	8	CD
cord-271701-tx0lqgff	163	13	)	)	-RRB-
cord-271701-tx0lqgff	163	14	complexes	complex	NNS
cord-271701-tx0lqgff	163	15	in	in	IN
cord-271701-tx0lqgff	163	16	the	the	DT
cord-271701-tx0lqgff	163	17	reaction	reaction	NN
cord-271701-tx0lqgff	163	18	by	by	IN
cord-271701-tx0lqgff	163	19	titrating	titrate	VBG
cord-271701-tx0lqgff	163	20	0	0	CD
cord-271701-tx0lqgff	163	21	-	-	SYM
cord-271701-tx0lqgff	163	22	100	100	CD
cord-271701-tx0lqgff	163	23	mM	mm	CD
cord-271701-tx0lqgff	163	24	into	into	IN
cord-271701-tx0lqgff	163	25	the	the	DT
cord-271701-tx0lqgff	163	26	reaction	reaction	NN
cord-271701-tx0lqgff	163	27	(	(	-LRB-
cord-271701-tx0lqgff	163	28	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	163	29	Figure	figure	NN
cord-271701-tx0lqgff	163	30	S2A	s2a	NN
cord-271701-tx0lqgff	163	31	)	)	-RRB-
cord-271701-tx0lqgff	163	32	and	and	CC
cord-271701-tx0lqgff	163	33	observed	observe	VBD
cord-271701-tx0lqgff	163	34	that	that	IN
cord-271701-tx0lqgff	163	35	the	the	DT
cord-271701-tx0lqgff	163	36	incorporation	incorporation	NN
cord-271701-tx0lqgff	163	37	levels	level	NNS
cord-271701-tx0lqgff	163	38	were	be	VBD
cord-271701-tx0lqgff	163	39	stable	stable	JJ
cord-271701-tx0lqgff	163	40	above	above	IN
cord-271701-tx0lqgff	163	41	1	1	CD
cord-271701-tx0lqgff	163	42	mM	mm	CD
cord-271701-tx0lqgff	163	43	(	(	-LRB-
cord-271701-tx0lqgff	163	44	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	163	45	Figure	Figure	NNP
cord-271701-tx0lqgff	163	46	S2B	S2B	NNP
cord-271701-tx0lqgff	163	47	)	)	-RRB-
cord-271701-tx0lqgff	163	48	,	,	,
cord-271701-tx0lqgff	163	49	suggesting	suggest	VBG
cord-271701-tx0lqgff	163	50	that	that	IN
cord-271701-tx0lqgff	163	51	these	these	DT
cord-271701-tx0lqgff	163	52	reactions	reaction	NNS
cord-271701-tx0lqgff	163	53	represent	represent	VBP
cord-271701-tx0lqgff	163	54	single	single	JJ
cord-271701-tx0lqgff	163	55	initiation	initiation	NN
cord-271701-tx0lqgff	163	56	-	-	HYPH
cord-271701-tx0lqgff	163	57	extension	extension	NN
cord-271701-tx0lqgff	163	58	events	event	NNS
cord-271701-tx0lqgff	163	59	.	.	.
cord-271701-tx0lqgff	164	1	We	-PRON-	PRP
cord-271701-tx0lqgff	164	2	next	next	RB
cord-271701-tx0lqgff	164	3	assessed	assess	VBD
cord-271701-tx0lqgff	164	4	whether	whether	IN
cord-271701-tx0lqgff	164	5	the	the	DT
cord-271701-tx0lqgff	164	6	activity	activity	NN
cord-271701-tx0lqgff	164	7	of	of	IN
cord-271701-tx0lqgff	164	8	nsp8	nsp8	NN
cord-271701-tx0lqgff	164	9	or	or	CC
cord-271701-tx0lqgff	164	10	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	164	11	+	+	SYM
cord-271701-tx0lqgff	164	12	8	8	CD
cord-271701-tx0lqgff	164	13	)	)	-RRB-
cord-271701-tx0lqgff	164	14	was	be	VBD
cord-271701-tx0lqgff	164	15	distributive	distributive	JJ
cord-271701-tx0lqgff	164	16	or	or	CC
cord-271701-tx0lqgff	164	17	processive	processive	JJ
cord-271701-tx0lqgff	164	18	by	by	IN
cord-271701-tx0lqgff	164	19	quantifying	quantify	VBG
cord-271701-tx0lqgff	164	20	the	the	DT
cord-271701-tx0lqgff	164	21	incorporated	incorporate	VBN
cord-271701-tx0lqgff	164	22	signal	signal	NN
cord-271701-tx0lqgff	164	23	in	in	IN
cord-271701-tx0lqgff	164	24	full	full	JJ
cord-271701-tx0lqgff	164	25	-	-	HYPH
cord-271701-tx0lqgff	164	26	length	length	NN
cord-271701-tx0lqgff	164	27	or	or	CC
cord-271701-tx0lqgff	164	28	longer	long	JJR
cord-271701-tx0lqgff	164	29	products	product	NNS
cord-271701-tx0lqgff	164	30	in	in	IN
cord-271701-tx0lqgff	164	31	the	the	DT
cord-271701-tx0lqgff	164	32	presence	presence	NN
cord-271701-tx0lqgff	164	33	of	of	IN
cord-271701-tx0lqgff	164	34	1	1	CD
cord-271701-tx0lqgff	164	35	mM	mm	CD
cord-271701-tx0lqgff	164	36	heparin	heparin	JJ
cord-271701-tx0lqgff	165	1	(	(	-LRB-
cord-271701-tx0lqgff	165	2	Figure	figure	NN
cord-271701-tx0lqgff	165	3	4C	4c	CD
cord-271701-tx0lqgff	165	4	)	)	-RRB-
cord-271701-tx0lqgff	165	5	.	.	.
cord-271701-tx0lqgff	166	1	As	as	IN
cord-271701-tx0lqgff	166	2	shown	show	VBN
cord-271701-tx0lqgff	166	3	in	in	IN
cord-271701-tx0lqgff	166	4	Figure	Figure	NNP
cord-271701-tx0lqgff	166	5	4D	4d	CD
cord-271701-tx0lqgff	166	6	,	,	,
cord-271701-tx0lqgff	166	7	66	66	CD
cord-271701-tx0lqgff	166	8	±	±	CD
cord-271701-tx0lqgff	166	9	4	4	CD
cord-271701-tx0lqgff	166	10	%	%	NN
cord-271701-tx0lqgff	166	11	(	(	-LRB-
cord-271701-tx0lqgff	166	12	mean	mean	VB
cord-271701-tx0lqgff	166	13	±	±	NNP
cord-271701-tx0lqgff	166	14	standard	standard	JJ
cord-271701-tx0lqgff	166	15	deviation	deviation	NN
cord-271701-tx0lqgff	166	16	)	)	-RRB-
cord-271701-tx0lqgff	166	17	of	of	IN
cord-271701-tx0lqgff	166	18	the	the	DT
cord-271701-tx0lqgff	166	19	nsp8	nsp8	JJ
cord-271701-tx0lqgff	166	20	products	product	NNS
cord-271701-tx0lqgff	166	21	were	be	VBD
cord-271701-tx0lqgff	166	22	full	full	JJ
cord-271701-tx0lqgff	166	23	length	length	NN
cord-271701-tx0lqgff	166	24	compared	compare	VBN
cord-271701-tx0lqgff	166	25	to	to	IN
cord-271701-tx0lqgff	166	26	61	61	CD
cord-271701-tx0lqgff	166	27	±	±	CD
cord-271701-tx0lqgff	166	28	2	2	CD
cord-271701-tx0lqgff	166	29	%	%	NN
cord-271701-tx0lqgff	166	30	of	of	IN
cord-271701-tx0lqgff	166	31	the	the	DT
cord-271701-tx0lqgff	166	32	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	166	33	+	+	SYM
cord-271701-tx0lqgff	166	34	8	8	CD
cord-271701-tx0lqgff	166	35	)	)	-RRB-
cord-271701-tx0lqgff	166	36	products	product	NNS
cord-271701-tx0lqgff	166	37	,	,	,
cord-271701-tx0lqgff	166	38	suggesting	suggest	VBG
cord-271701-tx0lqgff	166	39	that	that	IN
cord-271701-tx0lqgff	166	40	both	both	CC
cord-271701-tx0lqgff	166	41	the	the	DT
cord-271701-tx0lqgff	166	42	enzymes	enzyme	NNS
cord-271701-tx0lqgff	166	43	complexes	complex	NNS
cord-271701-tx0lqgff	166	44	are	be	VBP
cord-271701-tx0lqgff	166	45	mostly	mostly	RB
cord-271701-tx0lqgff	166	46	processive	processive	JJ
cord-271701-tx0lqgff	166	47	and	and	CC
cord-271701-tx0lqgff	166	48	that	that	IN
cord-271701-tx0lqgff	166	49	nsp7	nsp7	NNP
cord-271701-tx0lqgff	166	50	does	do	VBZ
cord-271701-tx0lqgff	166	51	not	not	RB
cord-271701-tx0lqgff	166	52	confer	confer	VB
cord-271701-tx0lqgff	166	53	additional	additional	JJ
cord-271701-tx0lqgff	166	54	processivity	processivity	NN
cord-271701-tx0lqgff	166	55	to	to	IN
cord-271701-tx0lqgff	166	56	nsp8	nsp8	NN
cord-271701-tx0lqgff	166	57	.	.	.
cord-271701-tx0lqgff	167	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	167	2	,	,	,
cord-271701-tx0lqgff	167	3	both	both	CC
cord-271701-tx0lqgff	167	4	nsp8	nsp8	NN
cord-271701-tx0lqgff	167	5	and	and	CC
cord-271701-tx0lqgff	167	6	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	167	7	+	+	SYM
cord-271701-tx0lqgff	167	8	8	8	CD
cord-271701-tx0lqgff	167	9	)	)	-RRB-
cord-271701-tx0lqgff	167	10	are	be	VBP
cord-271701-tx0lqgff	167	11	able	able	JJ
cord-271701-tx0lqgff	167	12	to	to	TO
cord-271701-tx0lqgff	167	13	extend	extend	VB
cord-271701-tx0lqgff	167	14	the	the	DT
cord-271701-tx0lqgff	167	15	RNA	RNA	NNP
cord-271701-tx0lqgff	167	16	primers	primer	NNS
cord-271701-tx0lqgff	167	17	beyond	beyond	IN
cord-271701-tx0lqgff	167	18	template	template	NN
cord-271701-tx0lqgff	167	19	length	length	NN
cord-271701-tx0lqgff	167	20	in	in	IN
cord-271701-tx0lqgff	167	21	the	the	DT
cord-271701-tx0lqgff	167	22	presence	presence	NN
cord-271701-tx0lqgff	167	23	of	of	IN
cord-271701-tx0lqgff	167	24	heparin	heparin	NN
cord-271701-tx0lqgff	167	25	(	(	-LRB-
cord-271701-tx0lqgff	167	26	Figure	Figure	NNP
cord-271701-tx0lqgff	167	27	4D	4D	NNP
cord-271701-tx0lqgff	167	28	and	and	CC
cord-271701-tx0lqgff	167	29	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	167	30	Figure	Figure	NNP
cord-271701-tx0lqgff	167	31	S2B	S2B	NNP
cord-271701-tx0lqgff	167	32	)	)	-RRB-
cord-271701-tx0lqgff	167	33	,	,	,
cord-271701-tx0lqgff	167	34	suggesting	suggest	VBG
cord-271701-tx0lqgff	167	35	that	that	IN
cord-271701-tx0lqgff	167	36	these	these	DT
cord-271701-tx0lqgff	167	37	extensions	extension	NNS
cord-271701-tx0lqgff	167	38	result	result	VBP
cord-271701-tx0lqgff	167	39	from	from	IN
cord-271701-tx0lqgff	167	40	terminal	terminal	JJ
cord-271701-tx0lqgff	167	41	transferase	transferase	NN
cord-271701-tx0lqgff	167	42	activity	activity	NN
cord-271701-tx0lqgff	167	43	and	and	CC
cord-271701-tx0lqgff	167	44	not	not	RB
cord-271701-tx0lqgff	167	45	from	from	IN
cord-271701-tx0lqgff	167	46	template	template	NN
cord-271701-tx0lqgff	167	47	switching	switching	NN
cord-271701-tx0lqgff	167	48	,	,	,
cord-271701-tx0lqgff	167	49	as	as	IN
cord-271701-tx0lqgff	167	50	was	be	VBD
cord-271701-tx0lqgff	167	51	previously	previously	RB
cord-271701-tx0lqgff	167	52	observed	observe	VBN
cord-271701-tx0lqgff	167	53	for	for	IN
cord-271701-tx0lqgff	167	54	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	167	55	3D	3D	NNP
cord-271701-tx0lqgff	167	56	pol	pol	NN
cord-271701-tx0lqgff	167	57	(	(	-LRB-
cord-271701-tx0lqgff	167	58	20	20	CD
cord-271701-tx0lqgff	167	59	)	)	-RRB-
cord-271701-tx0lqgff	167	60	.	.	.
cord-271701-tx0lqgff	168	1	Intrigued	intrigue	VBN
cord-271701-tx0lqgff	168	2	by	by	IN
cord-271701-tx0lqgff	168	3	the	the	DT
cord-271701-tx0lqgff	168	4	primer	primer	NN
cord-271701-tx0lqgff	168	5	extension	extension	NN
cord-271701-tx0lqgff	168	6	activity	activity	NN
cord-271701-tx0lqgff	168	7	of	of	IN
cord-271701-tx0lqgff	168	8	the	the	DT
cord-271701-tx0lqgff	168	9	SARS	SARS	NNP
cord-271701-tx0lqgff	168	10	-	-	HYPH
cord-271701-tx0lqgff	168	11	CoV	CoV	NNP
cord-271701-tx0lqgff	168	12	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	168	13	+	+	SYM
cord-271701-tx0lqgff	168	14	8	8	CD
cord-271701-tx0lqgff	168	15	)	)	-RRB-
cord-271701-tx0lqgff	168	16	complex	complex	NN
cord-271701-tx0lqgff	168	17	described	describe	VBN
cord-271701-tx0lqgff	168	18	above	above	RB
cord-271701-tx0lqgff	168	19	,	,	,
cord-271701-tx0lqgff	168	20	we	-PRON-	PRP
cord-271701-tx0lqgff	168	21	next	next	RB
cord-271701-tx0lqgff	168	22	designed	design	VBD
cord-271701-tx0lqgff	168	23	a	a	DT
cord-271701-tx0lqgff	168	24	set	set	NN
cord-271701-tx0lqgff	168	25	of	of	IN
cord-271701-tx0lqgff	168	26	mutations	mutation	NNS
cord-271701-tx0lqgff	168	27	to	to	TO
cord-271701-tx0lqgff	168	28	verify	verify	VB
cord-271701-tx0lqgff	168	29	that	that	IN
cord-271701-tx0lqgff	168	30	the	the	DT
cord-271701-tx0lqgff	168	31	activity	activity	NN
cord-271701-tx0lqgff	168	32	indeed	indeed	RB
cord-271701-tx0lqgff	168	33	was	be	VBD
cord-271701-tx0lqgff	168	34	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	168	35	+	+	SYM
cord-271701-tx0lqgff	168	36	8	8	CD
cord-271701-tx0lqgff	168	37	)	)	-RRB-
cord-271701-tx0lqgff	168	38	derived	derive	VBN
cord-271701-tx0lqgff	168	39	and	and	CC
cord-271701-tx0lqgff	168	40	to	to	TO
cord-271701-tx0lqgff	168	41	identify	identify	VB
cord-271701-tx0lqgff	168	42	the	the	DT
cord-271701-tx0lqgff	168	43	most	most	RBS
cord-271701-tx0lqgff	168	44	critical	critical	JJ
cord-271701-tx0lqgff	168	45	residues	residue	NNS
cord-271701-tx0lqgff	168	46	for	for	IN
cord-271701-tx0lqgff	168	47	activity	activity	NN
cord-271701-tx0lqgff	168	48	in	in	IN
cord-271701-tx0lqgff	168	49	the	the	DT
cord-271701-tx0lqgff	168	50	complex	complex	NN
cord-271701-tx0lqgff	168	51	.	.	.
cord-271701-tx0lqgff	169	1	We	-PRON-	PRP
cord-271701-tx0lqgff	169	2	first	first	RB
cord-271701-tx0lqgff	169	3	tested	test	VBD
cord-271701-tx0lqgff	169	4	RNA	RNA	NNP
cord-271701-tx0lqgff	169	5	-	-	HYPH
cord-271701-tx0lqgff	169	6	binding	bind	VBG
cord-271701-tx0lqgff	169	7	mutant	mutant	JJ
cord-271701-tx0lqgff	169	8	K58A	k58a	NN
cord-271701-tx0lqgff	169	9	(	(	-LRB-
cord-271701-tx0lqgff	169	10	Figure	figure	NN
cord-271701-tx0lqgff	169	11	2	2	CD
cord-271701-tx0lqgff	169	12	)	)	-RRB-
cord-271701-tx0lqgff	169	13	at	at	IN
cord-271701-tx0lqgff	169	14	varying	vary	VBG
cord-271701-tx0lqgff	169	15	concentrations	concentration	NNS
cord-271701-tx0lqgff	169	16	and	and	CC
cord-271701-tx0lqgff	169	17	observed	observe	VBD
cord-271701-tx0lqgff	169	18	a	a	DT
cord-271701-tx0lqgff	169	19	$	$	$
cord-271701-tx0lqgff	169	20	95	95	CD
cord-271701-tx0lqgff	169	21	%	%	NN
cord-271701-tx0lqgff	169	22	loss	loss	NN
cord-271701-tx0lqgff	169	23	of	of	IN
cord-271701-tx0lqgff	169	24	nucleotide	nucleotide	JJ
cord-271701-tx0lqgff	169	25	incorporation	incorporation	NN
cord-271701-tx0lqgff	169	26	activity	activity	NN
cord-271701-tx0lqgff	169	27	compared	compare	VBN
cord-271701-tx0lqgff	169	28	to	to	IN
cord-271701-tx0lqgff	169	29	the	the	DT
cord-271701-tx0lqgff	169	30	wild	wild	JJ
cord-271701-tx0lqgff	169	31	-	-	HYPH
cord-271701-tx0lqgff	169	32	type	type	NN
cord-271701-tx0lqgff	169	33	protein	protein	NN
cord-271701-tx0lqgff	169	34	(	(	-LRB-
cord-271701-tx0lqgff	169	35	Figure	figure	NN
cord-271701-tx0lqgff	169	36	5	5	CD
cord-271701-tx0lqgff	169	37	)	)	-RRB-
cord-271701-tx0lqgff	169	38	.	.	.
cord-271701-tx0lqgff	170	1	Other	other	JJ
cord-271701-tx0lqgff	170	2	likely	likely	JJ
cord-271701-tx0lqgff	170	3	candidates	candidate	NNS
cord-271701-tx0lqgff	170	4	for	for	IN
cord-271701-tx0lqgff	170	5	a	a	DT
cord-271701-tx0lqgff	170	6	direct	direct	JJ
cord-271701-tx0lqgff	170	7	role	role	NN
cord-271701-tx0lqgff	170	8	in	in	IN
cord-271701-tx0lqgff	170	9	RdRp	RdRp	NNP
cord-271701-tx0lqgff	170	10	catalysis	catalysis	NN
cord-271701-tx0lqgff	170	11	generally	generally	RB
cord-271701-tx0lqgff	170	12	are	be	VBP
cord-271701-tx0lqgff	170	13	Mg	Mg	NNP
cord-271701-tx0lqgff	170	14	2	2	CD
cord-271701-tx0lqgff	171	1	+	+	CC
cord-271701-tx0lqgff	172	1	-coordinating	-coordinate	VBG
cord-271701-tx0lqgff	172	2	aspartate	aspartate	NN
cord-271701-tx0lqgff	172	3	residues	residue	NNS
cord-271701-tx0lqgff	172	4	and	and	CC
cord-271701-tx0lqgff	172	5	lysine	lysine	NN
cord-271701-tx0lqgff	172	6	or	or	CC
cord-271701-tx0lqgff	172	7	histidine	histidine	NN
cord-271701-tx0lqgff	172	8	residues	residue	NNS
cord-271701-tx0lqgff	172	9	that	that	WDT
cord-271701-tx0lqgff	172	10	can	can	MD
cord-271701-tx0lqgff	172	11	function	function	VB
cord-271701-tx0lqgff	172	12	as	as	IN
cord-271701-tx0lqgff	172	13	general	general	JJ
cord-271701-tx0lqgff	172	14	acid	acid	NN
cord-271701-tx0lqgff	172	15	(	(	-LRB-
cord-271701-tx0lqgff	172	16	3	3	CD
cord-271701-tx0lqgff	172	17	)	)	-RRB-
cord-271701-tx0lqgff	172	18	.	.	.
cord-271701-tx0lqgff	173	1	In	in	IN
cord-271701-tx0lqgff	173	2	canonical	canonical	JJ
cord-271701-tx0lqgff	173	3	RNA	RNA	NNP
cord-271701-tx0lqgff	173	4	polymerases	polymerase	NNS
cord-271701-tx0lqgff	173	5	,	,	,
cord-271701-tx0lqgff	173	6	the	the	DT
cord-271701-tx0lqgff	173	7	aspartates	aspartate	NNS
cord-271701-tx0lqgff	173	8	commonly	commonly	RB
cord-271701-tx0lqgff	173	9	reside	reside	VBP
cord-271701-tx0lqgff	173	10	in	in	IN
cord-271701-tx0lqgff	173	11	motifs	motif	NNS
cord-271701-tx0lqgff	173	12	A	a	NN
cord-271701-tx0lqgff	173	13	and	and	CC
cord-271701-tx0lqgff	173	14	C	C	NNP
cord-271701-tx0lqgff	173	15	(	(	-LRB-
cord-271701-tx0lqgff	173	16	3	3	CD
cord-271701-tx0lqgff	173	17	,	,	,
cord-271701-tx0lqgff	173	18	21	21	CD
cord-271701-tx0lqgff	173	19	)	)	-RRB-
cord-271701-tx0lqgff	173	20	,	,	,
cord-271701-tx0lqgff	173	21	while	while	IN
cord-271701-tx0lqgff	173	22	in	in	IN
cord-271701-tx0lqgff	173	23	DNA	dna	NN
cord-271701-tx0lqgff	173	24	-	-	HYPH
cord-271701-tx0lqgff	173	25	dependent	dependent	JJ
cord-271701-tx0lqgff	173	26	RNA	RNA	NNP
cord-271701-tx0lqgff	173	27	primases	primase	NNS
cord-271701-tx0lqgff	173	28	they	-PRON-	PRP
cord-271701-tx0lqgff	173	29	are	be	VBP
cord-271701-tx0lqgff	173	30	usually	usually	RB
cord-271701-tx0lqgff	173	31	found	find	VBN
cord-271701-tx0lqgff	173	32	in	in	IN
cord-271701-tx0lqgff	173	33	a	a	DT
cord-271701-tx0lqgff	173	34	central	central	JJ
cord-271701-tx0lqgff	173	35	D	D	NNP
cord-271701-tx0lqgff	173	36	/	/	SYM
cord-271701-tx0lqgff	173	37	ExD	ExD	NNP
cord-271701-tx0lqgff	173	38	/	/	SYM
cord-271701-tx0lqgff	173	39	E	e	NN
cord-271701-tx0lqgff	173	40	motif	motif	NN
cord-271701-tx0lqgff	173	41	(	(	-LRB-
cord-271701-tx0lqgff	173	42	22	22	CD
cord-271701-tx0lqgff	173	43	)	)	-RRB-
cord-271701-tx0lqgff	173	44	.	.	.
cord-271701-tx0lqgff	174	1	Given	give	VBN
cord-271701-tx0lqgff	174	2	the	the	DT
cord-271701-tx0lqgff	174	3	absence	absence	NN
cord-271701-tx0lqgff	174	4	of	of	IN
cord-271701-tx0lqgff	174	5	classical	classical	JJ
cord-271701-tx0lqgff	174	6	RdRp	RdRp	NNP
cord-271701-tx0lqgff	174	7	A	A	NNP
cord-271701-tx0lqgff	174	8	and	and	CC
cord-271701-tx0lqgff	174	9	C	c	NN
cord-271701-tx0lqgff	174	10	motifs	motif	NNS
cord-271701-tx0lqgff	174	11	in	in	IN
cord-271701-tx0lqgff	174	12	the	the	DT
cord-271701-tx0lqgff	174	13	nsp8	nsp8	JJ
cord-271701-tx0lqgff	174	14	sequence	sequence	NN
cord-271701-tx0lqgff	174	15	(	(	-LRB-
cord-271701-tx0lqgff	174	16	12	12	CD
cord-271701-tx0lqgff	174	17	)	)	-RRB-
cord-271701-tx0lqgff	174	18	,	,	,
cord-271701-tx0lqgff	174	19	we	-PRON-	PRP
cord-271701-tx0lqgff	174	20	screened	screen	VBD
cord-271701-tx0lqgff	174	21	an	an	DT
cord-271701-tx0lqgff	174	22	alignment	alignment	NN
cord-271701-tx0lqgff	174	23	of	of	IN
cord-271701-tx0lqgff	174	24	CoV	CoV	NNP
cord-271701-tx0lqgff	174	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	174	26	sequences	sequence	NNS
cord-271701-tx0lqgff	174	27	for	for	IN
cord-271701-tx0lqgff	174	28	conserved	conserved	JJ
cord-271701-tx0lqgff	174	29	D	D	NNP
cord-271701-tx0lqgff	174	30	/	/	SYM
cord-271701-tx0lqgff	174	31	ExD	exd	NN
cord-271701-tx0lqgff	174	32	/	/	SYM
cord-271701-tx0lqgff	174	33	E	e	NN
cord-271701-tx0lqgff	174	34	motifs	motif	NNS
cord-271701-tx0lqgff	174	35	.	.	.
cord-271701-tx0lqgff	175	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	175	2	,	,	,
cord-271701-tx0lqgff	175	3	we	-PRON-	PRP
cord-271701-tx0lqgff	175	4	found	find	VBD
cord-271701-tx0lqgff	175	5	such	such	PDT
cord-271701-tx0lqgff	175	6	a	a	DT
cord-271701-tx0lqgff	175	7	motif	motif	NN
cord-271701-tx0lqgff	175	8	in	in	IN
cord-271701-tx0lqgff	175	9	both	both	CC
cord-271701-tx0lqgff	175	10	the	the	DT
cord-271701-tx0lqgff	175	11	N	N	NNP
cord-271701-tx0lqgff	175	12	-	-	HYPH
cord-271701-tx0lqgff	175	13	terminal	terminal	NN
cord-271701-tx0lqgff	175	14	and	and	CC
cord-271701-tx0lqgff	175	15	the	the	DT
cord-271701-tx0lqgff	175	16	C	c	NN
cord-271701-tx0lqgff	175	17	-	-	HYPH
cord-271701-tx0lqgff	175	18	terminal	terminal	NN
cord-271701-tx0lqgff	175	19	domain	domain	NN
cord-271701-tx0lqgff	175	20	(	(	-LRB-
cord-271701-tx0lqgff	175	21	Figure	figure	NN
cord-271701-tx0lqgff	175	22	5A	5a	NN
cord-271701-tx0lqgff	175	23	)	)	-RRB-
cord-271701-tx0lqgff	175	24	.	.	.
cord-271701-tx0lqgff	176	1	Subsequent	subsequent	JJ
cord-271701-tx0lqgff	176	2	alanine	alanine	JJ
cord-271701-tx0lqgff	176	3	substitution	substitution	NN
cord-271701-tx0lqgff	176	4	of	of	IN
cord-271701-tx0lqgff	176	5	the	the	DT
cord-271701-tx0lqgff	176	6	N	n	CD
cord-271701-tx0lqgff	176	7	-	-	HYPH
cord-271701-tx0lqgff	176	8	terminal	terminal	NN
cord-271701-tx0lqgff	176	9	D/	D/	NNP
cord-271701-tx0lqgff	176	10	ExD	ExD	NNP
cord-271701-tx0lqgff	176	11	/	/	SYM
cord-271701-tx0lqgff	176	12	E	e	NN
cord-271701-tx0lqgff	176	13	motif	motif	NN
cord-271701-tx0lqgff	176	14	,	,	,
cord-271701-tx0lqgff	176	15	composed	compose	VBN
cord-271701-tx0lqgff	176	16	of	of	IN
cord-271701-tx0lqgff	176	17	D50	D50	NNP
cord-271701-tx0lqgff	176	18	and	and	CC
cord-271701-tx0lqgff	176	19	D52	D52	NNP
cord-271701-tx0lqgff	176	20	in	in	IN
cord-271701-tx0lqgff	176	21	SARS	SARS	NNP
cord-271701-tx0lqgff	176	22	-	-	HYPH
cord-271701-tx0lqgff	176	23	CoV	CoV	NNP
cord-271701-tx0lqgff	176	24	,	,	,
cord-271701-tx0lqgff	176	25	greatly	greatly	RB
cord-271701-tx0lqgff	176	26	affected	affect	VBN
cord-271701-tx0lqgff	176	27	primer	primer	NN
cord-271701-tx0lqgff	176	28	extension	extension	NN
cord-271701-tx0lqgff	176	29	activity	activity	NN
cord-271701-tx0lqgff	176	30	on	on	IN
cord-271701-tx0lqgff	176	31	the	the	DT
cord-271701-tx0lqgff	176	32	CU	CU	NNP
cord-271701-tx0lqgff	176	33	10	10	CD
cord-271701-tx0lqgff	176	34	template	template	NN
cord-271701-tx0lqgff	176	35	as	as	IN
cord-271701-tx0lqgff	176	36	shown	show	VBN
cord-271701-tx0lqgff	176	37	in	in	IN
cord-271701-tx0lqgff	176	38	Figure	figure	NN
cord-271701-tx0lqgff	176	39	5C	5c	CD
cord-271701-tx0lqgff	176	40	.	.	.
cord-271701-tx0lqgff	177	1	Mutation	mutation	NN
cord-271701-tx0lqgff	177	2	of	of	IN
cord-271701-tx0lqgff	177	3	the	the	DT
cord-271701-tx0lqgff	177	4	downstream	downstream	JJ
cord-271701-tx0lqgff	177	5	domain	domain	NN
cord-271701-tx0lqgff	177	6	(	(	-LRB-
cord-271701-tx0lqgff	177	7	residues	residue	NNS
cord-271701-tx0lqgff	177	8	D161	D161	NNS
cord-271701-tx0lqgff	177	9	and	and	CC
cord-271701-tx0lqgff	177	10	D163	D163	NNP
cord-271701-tx0lqgff	177	11	in	in	IN
cord-271701-tx0lqgff	177	12	SARS	SARS	NNP
cord-271701-tx0lqgff	177	13	-	-	HYPH
cord-271701-tx0lqgff	177	14	CoV	CoV	NNP
cord-271701-tx0lqgff	177	15	)	)	-RRB-
cord-271701-tx0lqgff	177	16	,	,	,
cord-271701-tx0lqgff	177	17	however	however	RB
cord-271701-tx0lqgff	177	18	,	,	,
cord-271701-tx0lqgff	177	19	had	have	VBD
cord-271701-tx0lqgff	177	20	a	a	DT
cord-271701-tx0lqgff	177	21	much	much	RB
cord-271701-tx0lqgff	177	22	smaller	small	JJR
cord-271701-tx0lqgff	177	23	effect	effect	NN
cord-271701-tx0lqgff	177	24	on	on	IN
cord-271701-tx0lqgff	177	25	polymerase	polymerase	NN
cord-271701-tx0lqgff	177	26	activity	activity	NN
cord-271701-tx0lqgff	177	27	,	,	,
cord-271701-tx0lqgff	177	28	suggesting	suggest	VBG
cord-271701-tx0lqgff	177	29	that	that	IN
cord-271701-tx0lqgff	177	30	this	this	DT
cord-271701-tx0lqgff	177	31	C	c	NN
cord-271701-tx0lqgff	177	32	-	-	HYPH
cord-271701-tx0lqgff	177	33	terminal	terminal	NN
cord-271701-tx0lqgff	177	34	D	D	NNP
cord-271701-tx0lqgff	177	35	/	/	SYM
cord-271701-tx0lqgff	177	36	ExD	ExD	NNP
cord-271701-tx0lqgff	177	37	/	/	SYM
cord-271701-tx0lqgff	177	38	E	e	NN
cord-271701-tx0lqgff	177	39	motif	motif	NN
cord-271701-tx0lqgff	177	40	is	be	VBZ
cord-271701-tx0lqgff	177	41	not	not	RB
cord-271701-tx0lqgff	177	42	critical	critical	JJ
cord-271701-tx0lqgff	177	43	for	for	IN
cord-271701-tx0lqgff	177	44	catalysis	catalysis	NN
cord-271701-tx0lqgff	177	45	.	.	.
cord-271701-tx0lqgff	178	1	Controls	control	NNS
cord-271701-tx0lqgff	178	2	included	include	VBD
cord-271701-tx0lqgff	178	3	mutant	mutant	JJ
cord-271701-tx0lqgff	178	4	K58A	K58A	NNS
cord-271701-tx0lqgff	178	5	and	and	CC
cord-271701-tx0lqgff	178	6	a	a	DT
cord-271701-tx0lqgff	178	7	mutant	mutant	JJ
cord-271701-tx0lqgff	178	8	carrying	carry	VBG
cord-271701-tx0lqgff	178	9	a	a	DT
cord-271701-tx0lqgff	178	10	lysine	lysine	NN
cord-271701-tx0lqgff	178	11	-	-	HYPH
cord-271701-tx0lqgff	178	12	to	to	IN
cord-271701-tx0lqgff	178	13	-	-	HYPH
cord-271701-tx0lqgff	178	14	alanine	alanine	NN
cord-271701-tx0lqgff	178	15	substitution	substitution	NN
cord-271701-tx0lqgff	178	16	of	of	IN
cord-271701-tx0lqgff	178	17	the	the	DT
cord-271701-tx0lqgff	178	18	non	non	JJ
cord-271701-tx0lqgff	178	19	-	-	JJ
cord-271701-tx0lqgff	178	20	conserved	conserved	JJ
cord-271701-tx0lqgff	178	21	residue	residue	NN
cord-271701-tx0lqgff	178	22	127	127	CD
cord-271701-tx0lqgff	178	23	.	.	.
cord-271701-tx0lqgff	179	1	In	in	IN
cord-271701-tx0lqgff	179	2	line	line	NN
cord-271701-tx0lqgff	179	3	with	with	IN
cord-271701-tx0lqgff	179	4	the	the	DT
cord-271701-tx0lqgff	179	5	observation	observation	NN
cord-271701-tx0lqgff	179	6	of	of	IN
cord-271701-tx0lqgff	179	7	the	the	DT
cord-271701-tx0lqgff	179	8	U	U	NNP
cord-271701-tx0lqgff	179	9	20	20	CD
cord-271701-tx0lqgff	179	10	template	template	NN
cord-271701-tx0lqgff	179	11	and	and	CC
cord-271701-tx0lqgff	179	12	its	-PRON-	PRP$
cord-271701-tx0lqgff	179	13	conservation	conservation	NN
cord-271701-tx0lqgff	179	14	in	in	IN
cord-271701-tx0lqgff	179	15	CoVs	CoVs	NNP
cord-271701-tx0lqgff	179	16	,	,	,
cord-271701-tx0lqgff	179	17	the	the	DT
cord-271701-tx0lqgff	179	18	loss	loss	NN
cord-271701-tx0lqgff	179	19	of	of	IN
cord-271701-tx0lqgff	179	20	a	a	DT
cord-271701-tx0lqgff	179	21	lysine	lysine	NN
cord-271701-tx0lqgff	179	22	at	at	IN
cord-271701-tx0lqgff	179	23	position	position	NN
cord-271701-tx0lqgff	179	24	58	58	CD
cord-271701-tx0lqgff	179	25	resulted	result	VBD
cord-271701-tx0lqgff	179	26	in	in	IN
cord-271701-tx0lqgff	179	27	a	a	DT
cord-271701-tx0lqgff	179	28	near	near	JJ
cord-271701-tx0lqgff	179	29	complete	complete	JJ
cord-271701-tx0lqgff	179	30	loss	loss	NN
cord-271701-tx0lqgff	179	31	of	of	IN
cord-271701-tx0lqgff	179	32	RdRp	RdRp	NNP
cord-271701-tx0lqgff	179	33	activity	activity	NN
cord-271701-tx0lqgff	179	34	,	,	,
cord-271701-tx0lqgff	179	35	whereas	whereas	IN
cord-271701-tx0lqgff	179	36	mutation	mutation	NN
cord-271701-tx0lqgff	179	37	of	of	IN
cord-271701-tx0lqgff	179	38	K127	K127	NNP
cord-271701-tx0lqgff	179	39	positively	positively	RB
cord-271701-tx0lqgff	179	40	influenced	influence	VBD
cord-271701-tx0lqgff	179	41	RNA	RNA	NNP
cord-271701-tx0lqgff	179	42	synthesis	synthesis	NN
cord-271701-tx0lqgff	179	43	(	(	-LRB-
cord-271701-tx0lqgff	179	44	Figure	figure	NN
cord-271701-tx0lqgff	179	45	5	5	CD
cord-271701-tx0lqgff	179	46	)	)	-RRB-
cord-271701-tx0lqgff	179	47	.	.	.
cord-271701-tx0lqgff	179	48	 	 	_SP
cord-271701-tx0lqgff	180	1	As	as	IN
cord-271701-tx0lqgff	180	2	outlined	outline	VBN
cord-271701-tx0lqgff	180	3	above	above	RB
cord-271701-tx0lqgff	180	4	,	,	,
cord-271701-tx0lqgff	180	5	magnesium	magnesium	NN
cord-271701-tx0lqgff	180	6	ions	ion	NNS
cord-271701-tx0lqgff	180	7	are	be	VBP
cord-271701-tx0lqgff	180	8	well	well	RB
cord-271701-tx0lqgff	180	9	-	-	HYPH
cord-271701-tx0lqgff	180	10	known	know	VBN
cord-271701-tx0lqgff	180	11	cofactors	cofactor	NNS
cord-271701-tx0lqgff	180	12	of	of	IN
cord-271701-tx0lqgff	180	13	nucleic	nucleic	JJ
cord-271701-tx0lqgff	180	14	acid	acid	NN
cord-271701-tx0lqgff	180	15	polymerases	polymerase	NNS
cord-271701-tx0lqgff	180	16	and	and	CC
cord-271701-tx0lqgff	180	17	assist	assist	VB
cord-271701-tx0lqgff	180	18	in	in	IN
cord-271701-tx0lqgff	180	19	the	the	DT
cord-271701-tx0lqgff	180	20	coordination	coordination	NN
cord-271701-tx0lqgff	180	21	and	and	CC
cord-271701-tx0lqgff	180	22	activation	activation	NN
cord-271701-tx0lqgff	180	23	of	of	IN
cord-271701-tx0lqgff	180	24	incoming	incoming	JJ
cord-271701-tx0lqgff	180	25	nucleoside	nucleoside	NN
cord-271701-tx0lqgff	180	26	triphosphates	triphosphate	NNS
cord-271701-tx0lqgff	180	27	.	.	.
cord-271701-tx0lqgff	181	1	Also	also	RB
cord-271701-tx0lqgff	181	2	the	the	DT
cord-271701-tx0lqgff	181	3	activity	activity	NN
cord-271701-tx0lqgff	181	4	of	of	IN
cord-271701-tx0lqgff	181	5	SARS	SARS	NNP
cord-271701-tx0lqgff	181	6	-	-	HYPH
cord-271701-tx0lqgff	181	7	CoV	CoV	NNP
cord-271701-tx0lqgff	181	8	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	181	9	+	+	SYM
cord-271701-tx0lqgff	181	10	8	8	CD
cord-271701-tx0lqgff	181	11	)	)	-RRB-
cord-271701-tx0lqgff	181	12	was	be	VBD
cord-271701-tx0lqgff	181	13	found	find	VBN
cord-271701-tx0lqgff	181	14	to	to	TO
cord-271701-tx0lqgff	181	15	be	be	VB
cord-271701-tx0lqgff	181	16	positively	positively	RB
cord-271701-tx0lqgff	181	17	correlated	correlate	VBN
cord-271701-tx0lqgff	181	18	with	with	IN
cord-271701-tx0lqgff	181	19	the	the	DT
cord-271701-tx0lqgff	181	20	Mg	Mg	NNP
cord-271701-tx0lqgff	181	21	2	2	CD
cord-271701-tx0lqgff	181	22	+	+	CC
cord-271701-tx0lqgff	181	23	concentration	concentration	NN
cord-271701-tx0lqgff	181	24	,	,	,
cord-271701-tx0lqgff	181	25	albeit	albeit	IN
cord-271701-tx0lqgff	181	26	with	with	IN
cord-271701-tx0lqgff	181	27	a	a	DT
cord-271701-tx0lqgff	181	28	broad	broad	JJ
cord-271701-tx0lqgff	181	29	optimum	optimum	JJ
cord-271701-tx0lqgff	181	30	running	running	NN
cord-271701-tx0lqgff	181	31	from	from	IN
cord-271701-tx0lqgff	181	32	4	4	CD
cord-271701-tx0lqgff	181	33	-	-	SYM
cord-271701-tx0lqgff	181	34	10	10	CD
cord-271701-tx0lqgff	181	35	mM	mm	CD
cord-271701-tx0lqgff	181	36	(	(	-LRB-
cord-271701-tx0lqgff	181	37	Figure	figure	NN
cord-271701-tx0lqgff	181	38	6A	6a	CD
cord-271701-tx0lqgff	181	39	)	)	-RRB-
cord-271701-tx0lqgff	181	40	.	.	.
cord-271701-tx0lqgff	182	1	At	at	IN
cord-271701-tx0lqgff	182	2	this	this	DT
cord-271701-tx0lqgff	182	3	optimum	optimum	NN
cord-271701-tx0lqgff	182	4	,	,	,
cord-271701-tx0lqgff	182	5	nsp(7	nsp(7	IN
cord-271701-tx0lqgff	182	6	+	+	SYM
cord-271701-tx0lqgff	182	7	8	8	CD
cord-271701-tx0lqgff	182	8	)	)	-RRB-
cord-271701-tx0lqgff	182	9	incorporates	incorporate	VBZ
cord-271701-tx0lqgff	182	10	$	$	$
cord-271701-tx0lqgff	182	11	1	1	CD
cord-271701-tx0lqgff	182	12	mM	mm	CD
cord-271701-tx0lqgff	182	13	NMP	NMP	NNP
cord-271701-tx0lqgff	182	14	into	into	IN
cord-271701-tx0lqgff	182	15	the	the	DT
cord-271701-tx0lqgff	182	16	primed	prime	VBN
cord-271701-tx0lqgff	182	17	template	template	NN
cord-271701-tx0lqgff	182	18	per	per	IN
cord-271701-tx0lqgff	182	19	mM	mm	CD
cord-271701-tx0lqgff	182	20	of	of	IN
cord-271701-tx0lqgff	182	21	monomeric	monomeric	JJ
cord-271701-tx0lqgff	182	22	nsp7	nsp7	NN
cord-271701-tx0lqgff	182	23	and	and	CC
cord-271701-tx0lqgff	182	24	nsp8	nsp8	NN
cord-271701-tx0lqgff	182	25	present	present	JJ
cord-271701-tx0lqgff	182	26	in	in	IN
cord-271701-tx0lqgff	182	27	the	the	DT
cord-271701-tx0lqgff	182	28	reaction	reaction	NN
cord-271701-tx0lqgff	182	29	.	.	.
cord-271701-tx0lqgff	183	1	Similar	similar	JJ
cord-271701-tx0lqgff	183	2	to	to	IN
cord-271701-tx0lqgff	183	3	the	the	DT
cord-271701-tx0lqgff	183	4	presence	presence	NN
cord-271701-tx0lqgff	183	5	of	of	IN
cord-271701-tx0lqgff	183	6	divalent	divalent	JJ
cord-271701-tx0lqgff	183	7	cations	cation	NNS
cord-271701-tx0lqgff	183	8	,	,	,
cord-271701-tx0lqgff	183	9	the	the	DT
cord-271701-tx0lqgff	183	10	pH	pH	NNP
cord-271701-tx0lqgff	183	11	greatly	greatly	RB
cord-271701-tx0lqgff	183	12	affects	affect	VBZ
cord-271701-tx0lqgff	183	13	the	the	DT
cord-271701-tx0lqgff	183	14	activity	activity	NN
cord-271701-tx0lqgff	183	15	of	of	IN
cord-271701-tx0lqgff	183	16	RdRps	RdRps	NNP
cord-271701-tx0lqgff	183	17	and	and	CC
cord-271701-tx0lqgff	183	18	has	have	VBZ
cord-271701-tx0lqgff	183	19	been	be	VBN
cord-271701-tx0lqgff	183	20	shown	show	VBN
cord-271701-tx0lqgff	183	21	to	to	TO
cord-271701-tx0lqgff	183	22	play	play	VB
cord-271701-tx0lqgff	183	23	a	a	DT
cord-271701-tx0lqgff	183	24	role	role	NN
cord-271701-tx0lqgff	183	25	in	in	IN
cord-271701-tx0lqgff	183	26	both	both	DT
cord-271701-tx0lqgff	183	27	catalysis	catalysis	NN
cord-271701-tx0lqgff	183	28	and	and	CC
cord-271701-tx0lqgff	183	29	fidelity	fidelity	NN
cord-271701-tx0lqgff	183	30	(	(	-LRB-
cord-271701-tx0lqgff	183	31	3	3	CD
cord-271701-tx0lqgff	183	32	,	,	,
cord-271701-tx0lqgff	183	33	23	23	CD
cord-271701-tx0lqgff	183	34	)	)	-RRB-
cord-271701-tx0lqgff	183	35	.	.	.
cord-271701-tx0lqgff	184	1	To	to	TO
cord-271701-tx0lqgff	184	2	investigate	investigate	VB
cord-271701-tx0lqgff	184	3	the	the	DT
cord-271701-tx0lqgff	184	4	influence	influence	NN
cord-271701-tx0lqgff	184	5	of	of	IN
cord-271701-tx0lqgff	184	6	the	the	DT
cord-271701-tx0lqgff	184	7	pH	pH	NNP
cord-271701-tx0lqgff	184	8	on	on	IN
cord-271701-tx0lqgff	184	9	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	184	10	+	+	SYM
cord-271701-tx0lqgff	184	11	8	8	CD
cord-271701-tx0lqgff	184	12	)	)	-RRB-
cord-271701-tx0lqgff	184	13	,	,	,
cord-271701-tx0lqgff	184	14	we	-PRON-	PRP
cord-271701-tx0lqgff	184	15	tested	test	VBD
cord-271701-tx0lqgff	184	16	the	the	DT
cord-271701-tx0lqgff	184	17	activity	activity	NN
cord-271701-tx0lqgff	184	18	of	of	IN
cord-271701-tx0lqgff	184	19	the	the	DT
cord-271701-tx0lqgff	184	20	complex	complex	NN
cord-271701-tx0lqgff	184	21	in	in	IN
cord-271701-tx0lqgff	184	22	a	a	DT
cord-271701-tx0lqgff	184	23	pH	pH	NNP
cord-271701-tx0lqgff	184	24	range	range	NN
cord-271701-tx0lqgff	184	25	of	of	IN
cord-271701-tx0lqgff	184	26	6	6	CD
cord-271701-tx0lqgff	184	27	-	-	SYM
cord-271701-tx0lqgff	184	28	11	11	CD
cord-271701-tx0lqgff	184	29	.	.	.
cord-271701-tx0lqgff	185	1	As	as	IN
cord-271701-tx0lqgff	185	2	shown	show	VBN
cord-271701-tx0lqgff	185	3	in	in	IN
cord-271701-tx0lqgff	185	4	Figure	figure	NN
cord-271701-tx0lqgff	185	5	6C	6c	NN
cord-271701-tx0lqgff	185	6	,	,	,
cord-271701-tx0lqgff	185	7	we	-PRON-	PRP
cord-271701-tx0lqgff	185	8	observed	observe	VBD
cord-271701-tx0lqgff	185	9	a	a	DT
cord-271701-tx0lqgff	185	10	sharp	sharp	JJ
cord-271701-tx0lqgff	185	11	optimum	optimum	NN
cord-271701-tx0lqgff	185	12	at	at	IN
cord-271701-tx0lqgff	185	13	pH	pH	NNP
cord-271701-tx0lqgff	185	14	9.5	9.5	CD
cord-271701-tx0lqgff	185	15	,	,	,
cord-271701-tx0lqgff	185	16	which	which	WDT
cord-271701-tx0lqgff	185	17	is	be	VBZ
cord-271701-tx0lqgff	185	18	considerably	considerably	RB
cord-271701-tx0lqgff	185	19	higher	high	JJR
cord-271701-tx0lqgff	185	20	than	than	IN
cord-271701-tx0lqgff	185	21	the	the	DT
cord-271701-tx0lqgff	185	22	optimum	optimum	NN
cord-271701-tx0lqgff	185	23	that	that	WDT
cord-271701-tx0lqgff	185	24	was	be	VBD
cord-271701-tx0lqgff	185	25	previously	previously	RB
cord-271701-tx0lqgff	185	26	observed	observe	VBN
cord-271701-tx0lqgff	185	27	for	for	IN
cord-271701-tx0lqgff	185	28	the	the	DT
cord-271701-tx0lqgff	185	29	SARS	SARS	NNP
cord-271701-tx0lqgff	185	30	-	-	HYPH
cord-271701-tx0lqgff	185	31	CoV	CoV	NNP
cord-271701-tx0lqgff	185	32	nsp12-RdRp	nsp12-RdRp	NNP
cord-271701-tx0lqgff	185	33	and	and	CC
cord-271701-tx0lqgff	185	34	the	the	DT
cord-271701-tx0lqgff	185	35	His	-PRON-	PRP$
cord-271701-tx0lqgff	185	36	-	-	HYPH
cord-271701-tx0lqgff	185	37	nsp8	nsp8	NN
cord-271701-tx0lqgff	185	38	homomer	homomer	NN
cord-271701-tx0lqgff	185	39	(	(	-LRB-
cord-271701-tx0lqgff	185	40	pH	pH	NNP
cord-271701-tx0lqgff	185	41	optimum	optimum	NNP
cord-271701-tx0lqgff	185	42	7.5	7.5	CD
cord-271701-tx0lqgff	185	43	and	and	CC
cord-271701-tx0lqgff	185	44	8.0	8.0	CD
cord-271701-tx0lqgff	185	45	,	,	,
cord-271701-tx0lqgff	185	46	respectively	respectively	RB
cord-271701-tx0lqgff	185	47	)	)	-RRB-
cord-271701-tx0lqgff	185	48	(	(	-LRB-
cord-271701-tx0lqgff	185	49	10	10	CD
cord-271701-tx0lqgff	185	50	,	,	,
cord-271701-tx0lqgff	185	51	12	12	CD
cord-271701-tx0lqgff	185	52	)	)	-RRB-
cord-271701-tx0lqgff	185	53	.	.	.
cord-271701-tx0lqgff	186	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	186	2	,	,	,
cord-271701-tx0lqgff	186	3	the	the	DT
cord-271701-tx0lqgff	186	4	primer	primer	NN
cord-271701-tx0lqgff	186	5	extension	extension	NN
cord-271701-tx0lqgff	186	6	activity	activity	NN
cord-271701-tx0lqgff	186	7	of	of	IN
cord-271701-tx0lqgff	186	8	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	186	9	+	+	SYM
cord-271701-tx0lqgff	186	10	8	8	CD
cord-271701-tx0lqgff	186	11	)	)	-RRB-
cord-271701-tx0lqgff	186	12	did	do	VBD
cord-271701-tx0lqgff	186	13	not	not	RB
cord-271701-tx0lqgff	186	14	require	require	VB
cord-271701-tx0lqgff	186	15	manganese	manganese	NN
cord-271701-tx0lqgff	186	16	ions	ion	NNS
cord-271701-tx0lqgff	186	17	as	as	IN
cord-271701-tx0lqgff	186	18	was	be	VBD
cord-271701-tx0lqgff	186	19	previously	previously	RB
cord-271701-tx0lqgff	186	20	reported	report	VBN
cord-271701-tx0lqgff	186	21	for	for	IN
cord-271701-tx0lqgff	186	22	the	the	DT
cord-271701-tx0lqgff	186	23	His	-PRON-	PRP$
cord-271701-tx0lqgff	186	24	-	-	HYPH
cord-271701-tx0lqgff	186	25	nsp8	nsp8	NN
cord-271701-tx0lqgff	186	26	homomer	homomer	NN
cord-271701-tx0lqgff	186	27	(	(	-LRB-
cord-271701-tx0lqgff	186	28	12	12	CD
cord-271701-tx0lqgff	186	29	)	)	-RRB-
cord-271701-tx0lqgff	186	30	.	.	.
cord-271701-tx0lqgff	187	1	In	in	IN
cord-271701-tx0lqgff	187	2	fact	fact	NN
cord-271701-tx0lqgff	187	3	,	,	,
cord-271701-tx0lqgff	187	4	similar	similar	JJ
cord-271701-tx0lqgff	187	5	to	to	IN
cord-271701-tx0lqgff	187	6	the	the	DT
cord-271701-tx0lqgff	187	7	SARS	SARS	NNP
cord-271701-tx0lqgff	187	8	-	-	HYPH
cord-271701-tx0lqgff	187	9	CoV	CoV	NNP
cord-271701-tx0lqgff	187	10	nsp12-RdRp	nsp12-RdRp	NNP
cord-271701-tx0lqgff	187	11	(	(	-LRB-
cord-271701-tx0lqgff	187	12	10	10	CD
cord-271701-tx0lqgff	187	13	)	)	-RRB-
cord-271701-tx0lqgff	187	14	,	,	,
cord-271701-tx0lqgff	187	15	the	the	DT
cord-271701-tx0lqgff	187	16	addition	addition	NN
cord-271701-tx0lqgff	187	17	of	of	IN
cord-271701-tx0lqgff	187	18	Mn	Mn	NNP
cord-271701-tx0lqgff	187	19	2	2	CD
cord-271701-tx0lqgff	187	20	+	+	SYM
cord-271701-tx0lqgff	187	21	was	be	VBD
cord-271701-tx0lqgff	187	22	found	find	VBN
cord-271701-tx0lqgff	187	23	to	to	TO
cord-271701-tx0lqgff	187	24	reduce	reduce	VB
cord-271701-tx0lqgff	187	25	the	the	DT
cord-271701-tx0lqgff	187	26	fidelity	fidelity	NN
cord-271701-tx0lqgff	187	27	of	of	IN
cord-271701-tx0lqgff	187	28	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	187	29	+	+	SYM
cord-271701-tx0lqgff	187	30	8	8	CD
cord-271701-tx0lqgff	187	31	)	)	-RRB-
cord-271701-tx0lqgff	187	32	and	and	CC
cord-271701-tx0lqgff	187	33	induce	induce	VB
cord-271701-tx0lqgff	187	34	both	both	DT
cord-271701-tx0lqgff	187	35	transversional	transversional	JJ
cord-271701-tx0lqgff	187	36	and	and	CC
cord-271701-tx0lqgff	187	37	transitional	transitional	JJ
cord-271701-tx0lqgff	187	38	misincorporations	misincorporation	NNS
cord-271701-tx0lqgff	187	39	in	in	IN
cord-271701-tx0lqgff	187	40	a	a	DT
cord-271701-tx0lqgff	187	41	pulse	pulse	NN
cord-271701-tx0lqgff	187	42	-	-	HYPH
cord-271701-tx0lqgff	187	43	chase	chase	NN
cord-271701-tx0lqgff	187	44	experiment	experiment	NN
cord-271701-tx0lqgff	187	45	(	(	-LRB-
cord-271701-tx0lqgff	187	46	Figure	Figure	NNP
cord-271701-tx0lqgff	187	47	6E	6E	NNP
cord-271701-tx0lqgff	187	48	and	and	CC
cord-271701-tx0lqgff	187	49	F	F	NNP
cord-271701-tx0lqgff	187	50	)	)	-RRB-
cord-271701-tx0lqgff	187	51	.	.	.
cord-271701-tx0lqgff	188	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	188	2	,	,	,
cord-271701-tx0lqgff	188	3	the	the	DT
cord-271701-tx0lqgff	188	4	assay	assay	NN
cord-271701-tx0lqgff	188	5	also	also	RB
cord-271701-tx0lqgff	188	6	revealed	reveal	VBD
cord-271701-tx0lqgff	188	7	a	a	DT
cord-271701-tx0lqgff	188	8	discrimination	discrimination	NN
cord-271701-tx0lqgff	188	9	against	against	IN
cord-271701-tx0lqgff	188	10	the	the	DT
cord-271701-tx0lqgff	188	11	widely	widely	RB
cord-271701-tx0lqgff	188	12	Figure	figure	NN
cord-271701-tx0lqgff	188	13	5	5	CD
cord-271701-tx0lqgff	188	14	.	.	.
cord-271701-tx0lqgff	189	1	Mutagenesis	Mutagenesis	NNP
cord-271701-tx0lqgff	189	2	of	of	IN
cord-271701-tx0lqgff	189	3	SARS	SARS	NNP
cord-271701-tx0lqgff	189	4	-	-	HYPH
cord-271701-tx0lqgff	189	5	CoV	CoV	NNP
cord-271701-tx0lqgff	189	6	nsp8	nsp8	NN
cord-271701-tx0lqgff	189	7	.	.	.
cord-271701-tx0lqgff	190	1	(	(	-LRB-
cord-271701-tx0lqgff	190	2	A	a	DT
cord-271701-tx0lqgff	190	3	)	)	-RRB-
cord-271701-tx0lqgff	190	4	Alignment	alignment	NN
cord-271701-tx0lqgff	190	5	of	of	IN
cord-271701-tx0lqgff	190	6	nsp8	nsp8	JJ
cord-271701-tx0lqgff	190	7	sequences	sequence	NNS
cord-271701-tx0lqgff	190	8	from	from	IN
cord-271701-tx0lqgff	190	9	representative	representative	NNP
cord-271701-tx0lqgff	190	10	alpha-	alpha-	NN
cord-271701-tx0lqgff	190	11	,	,	,
cord-271701-tx0lqgff	190	12	beta	beta	NN
cord-271701-tx0lqgff	190	13	-	-	HYPH
cord-271701-tx0lqgff	190	14	and	and	CC
cord-271701-tx0lqgff	190	15	gammacoronaviruses	gammacoronaviruse	NNS
cord-271701-tx0lqgff	190	16	.	.	.
cord-271701-tx0lqgff	191	1	Fully	fully	RB
cord-271701-tx0lqgff	191	2	conserved	conserve	VBN
cord-271701-tx0lqgff	191	3	residues	residue	NNS
cord-271701-tx0lqgff	191	4	are	be	VBP
cord-271701-tx0lqgff	191	5	shaded	shade	VBN
cord-271701-tx0lqgff	191	6	red	red	JJ
cord-271701-tx0lqgff	191	7	,	,	,
cord-271701-tx0lqgff	191	8	while	while	IN
cord-271701-tx0lqgff	191	9	partially	partially	RB
cord-271701-tx0lqgff	191	10	conserved	conserve	VBN
cord-271701-tx0lqgff	191	11	residues	residue	NNS
cord-271701-tx0lqgff	191	12	are	be	VBP
cord-271701-tx0lqgff	191	13	boxed	box	VBN
cord-271701-tx0lqgff	191	14	.	.	.
cord-271701-tx0lqgff	192	1	The	the	DT
cord-271701-tx0lqgff	192	2	residues	residue	NNS
cord-271701-tx0lqgff	192	3	targeted	target	VBN
cord-271701-tx0lqgff	192	4	by	by	IN
cord-271701-tx0lqgff	192	5	mutagenesis	mutagenesi	NNS
cord-271701-tx0lqgff	192	6	are	be	VBP
cord-271701-tx0lqgff	192	7	indicated	indicate	VBN
cord-271701-tx0lqgff	192	8	with	with	IN
cord-271701-tx0lqgff	192	9	asterisks	asterisk	NNS
cord-271701-tx0lqgff	192	10	.	.	.
cord-271701-tx0lqgff	193	1	Please	please	UH
cord-271701-tx0lqgff	193	2	see	see	VB
cord-271701-tx0lqgff	193	3	'	'	''
cord-271701-tx0lqgff	193	4	Material	Material	NNP
cord-271701-tx0lqgff	193	5	and	and	CC
cord-271701-tx0lqgff	193	6	Methods	Methods	NNPS
cord-271701-tx0lqgff	193	7	'	'	POS
cord-271701-tx0lqgff	193	8	section	section	NN
cord-271701-tx0lqgff	193	9	for	for	IN
cord-271701-tx0lqgff	193	10	the	the	DT
cord-271701-tx0lqgff	193	11	Genbank	Genbank	NNP
cord-271701-tx0lqgff	193	12	accession	accession	NN
cord-271701-tx0lqgff	193	13	numbers	number	NNS
cord-271701-tx0lqgff	193	14	associated	associate	VBN
cord-271701-tx0lqgff	193	15	with	with	IN
cord-271701-tx0lqgff	193	16	the	the	DT
cord-271701-tx0lqgff	193	17	presented	present	VBN
cord-271701-tx0lqgff	193	18	sequences	sequence	NNS
cord-271701-tx0lqgff	193	19	.	.	.
cord-271701-tx0lqgff	194	1	(	(	-LRB-
cord-271701-tx0lqgff	194	2	B	b	NN
cord-271701-tx0lqgff	194	3	)	)	-RRB-
cord-271701-tx0lqgff	195	1	To	to	TO
cord-271701-tx0lqgff	195	2	verify	verify	VB
cord-271701-tx0lqgff	195	3	that	that	IN
cord-271701-tx0lqgff	195	4	the	the	DT
cord-271701-tx0lqgff	195	5	observed	observed	JJ
cord-271701-tx0lqgff	195	6	extension	extension	NN
cord-271701-tx0lqgff	195	7	activity	activity	NN
cord-271701-tx0lqgff	195	8	was	be	VBD
cord-271701-tx0lqgff	195	9	nsp8-dependent	nsp8-dependent	NNP
cord-271701-tx0lqgff	195	10	,	,	,
cord-271701-tx0lqgff	195	11	we	-PRON-	PRP
cord-271701-tx0lqgff	195	12	tested	test	VBD
cord-271701-tx0lqgff	195	13	the	the	DT
cord-271701-tx0lqgff	195	14	incorporation	incorporation	NN
cord-271701-tx0lqgff	195	15	of	of	IN
cord-271701-tx0lqgff	195	16	AMP	amp	NN
cord-271701-tx0lqgff	195	17	into	into	IN
cord-271701-tx0lqgff	195	18	the	the	DT
cord-271701-tx0lqgff	195	19	primed	prime	VBN
cord-271701-tx0lqgff	195	20	U	u	NN
cord-271701-tx0lqgff	195	21	20	20	CD
cord-271701-tx0lqgff	195	22	template	template	NN
cord-271701-tx0lqgff	195	23	by	by	IN
cord-271701-tx0lqgff	195	24	1	1	CD
cord-271701-tx0lqgff	195	25	,	,	,
cord-271701-tx0lqgff	195	26	5	5	CD
cord-271701-tx0lqgff	195	27	or	or	CC
cord-271701-tx0lqgff	195	28	10	10	CD
cord-271701-tx0lqgff	195	29	mM	mm	CD
cord-271701-tx0lqgff	195	30	of	of	IN
cord-271701-tx0lqgff	195	31	wild	wild	JJ
cord-271701-tx0lqgff	195	32	-	-	HYPH
cord-271701-tx0lqgff	195	33	type	type	NN
cord-271701-tx0lqgff	195	34	nsp8	nsp8	NN
cord-271701-tx0lqgff	195	35	or	or	CC
cord-271701-tx0lqgff	195	36	template	template	NN
cord-271701-tx0lqgff	195	37	-	-	HYPH
cord-271701-tx0lqgff	195	38	binding	bind	VBG
cord-271701-tx0lqgff	195	39	mutant	mutant	JJ
cord-271701-tx0lqgff	195	40	K58A.	K58A.	NNP
cord-271701-tx0lqgff	195	41	Mutation	Mutation	NNP
cord-271701-tx0lqgff	195	42	of	of	IN
cord-271701-tx0lqgff	195	43	K58	K58	NNP
cord-271701-tx0lqgff	195	44	resulted	result	VBD
cord-271701-tx0lqgff	195	45	in	in	IN
cord-271701-tx0lqgff	195	46	a	a	DT
cord-271701-tx0lqgff	195	47	$	$	$
cord-271701-tx0lqgff	195	48	95	95	CD
cord-271701-tx0lqgff	195	49	%	%	NN
cord-271701-tx0lqgff	195	50	reduction	reduction	NN
cord-271701-tx0lqgff	195	51	of	of	IN
cord-271701-tx0lqgff	195	52	AMP	amp	NN
cord-271701-tx0lqgff	195	53	incorporation	incorporation	NN
cord-271701-tx0lqgff	195	54	.	.	.
cord-271701-tx0lqgff	196	1	(	(	-LRB-
cord-271701-tx0lqgff	196	2	C	C	NNP
cord-271701-tx0lqgff	196	3	)	)	-RRB-
cord-271701-tx0lqgff	197	1	To	to	TO
cord-271701-tx0lqgff	197	2	assess	assess	VB
cord-271701-tx0lqgff	197	3	the	the	DT
cord-271701-tx0lqgff	197	4	importance	importance	NN
cord-271701-tx0lqgff	197	5	of	of	IN
cord-271701-tx0lqgff	197	6	the	the	DT
cord-271701-tx0lqgff	197	7	two	two	CD
cord-271701-tx0lqgff	197	8	D	d	NN
cord-271701-tx0lqgff	197	9	/	/	SYM
cord-271701-tx0lqgff	197	10	ExD	exd	NN
cord-271701-tx0lqgff	197	11	/	/	SYM
cord-271701-tx0lqgff	197	12	E	e	NN
cord-271701-tx0lqgff	197	13	motifs	motif	NNS
cord-271701-tx0lqgff	197	14	in	in	IN
cord-271701-tx0lqgff	197	15	nsp8	nsp8	NN
cord-271701-tx0lqgff	197	16	,	,	,
cord-271701-tx0lqgff	197	17	we	-PRON-	PRP
cord-271701-tx0lqgff	197	18	engineered	engineer	VBD
cord-271701-tx0lqgff	197	19	alanine	alanine	JJ
cord-271701-tx0lqgff	197	20	substitution	substitution	NN
cord-271701-tx0lqgff	197	21	mutants	mutant	NNS
cord-271701-tx0lqgff	197	22	of	of	IN
cord-271701-tx0lqgff	197	23	these	these	DT
cord-271701-tx0lqgff	197	24	residues	residue	NNS
cord-271701-tx0lqgff	197	25	and	and	CC
cord-271701-tx0lqgff	197	26	tested	test	VBD
cord-271701-tx0lqgff	197	27	their	-PRON-	PRP$
cord-271701-tx0lqgff	197	28	primer	primer	NN
cord-271701-tx0lqgff	197	29	extension	extension	NN
cord-271701-tx0lqgff	197	30	activity	activity	NN
cord-271701-tx0lqgff	197	31	on	on	IN
cord-271701-tx0lqgff	197	32	the	the	DT
cord-271701-tx0lqgff	197	33	primed	prime	VBN
cord-271701-tx0lqgff	197	34	UC	UC	NNP
cord-271701-tx0lqgff	197	35	10	10	CD
cord-271701-tx0lqgff	197	36	template	template	NN
cord-271701-tx0lqgff	197	37	(	(	-LRB-
cord-271701-tx0lqgff	197	38	see	see	VB
cord-271701-tx0lqgff	197	39	Figure	figure	NN
cord-271701-tx0lqgff	197	40	4	4	CD
cord-271701-tx0lqgff	197	41	)	)	-RRB-
cord-271701-tx0lqgff	197	42	.	.	.
cord-271701-tx0lqgff	198	1	Reactions	reaction	NNS
cord-271701-tx0lqgff	198	2	were	be	VBD
cord-271701-tx0lqgff	198	3	stopped	stop	VBN
cord-271701-tx0lqgff	198	4	after	after	IN
cord-271701-tx0lqgff	198	5	60	60	CD
cord-271701-tx0lqgff	198	6	min	min	NN
cord-271701-tx0lqgff	198	7	and	and	CC
cord-271701-tx0lqgff	198	8	compared	compare	VBN
cord-271701-tx0lqgff	198	9	to	to	IN
cord-271701-tx0lqgff	198	10	the	the	DT
cord-271701-tx0lqgff	198	11	activity	activity	NN
cord-271701-tx0lqgff	198	12	of	of	IN
cord-271701-tx0lqgff	198	13	the	the	DT
cord-271701-tx0lqgff	198	14	wild	wild	JJ
cord-271701-tx0lqgff	198	15	-	-	HYPH
cord-271701-tx0lqgff	198	16	type	type	NN
cord-271701-tx0lqgff	198	17	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	198	18	+	+	SYM
cord-271701-tx0lqgff	198	19	8	8	CD
cord-271701-tx0lqgff	198	20	)	)	-RRB-
cord-271701-tx0lqgff	198	21	complex	complex	VB
cord-271701-tx0lqgff	198	22	on	on	IN
cord-271701-tx0lqgff	198	23	a	a	DT
cord-271701-tx0lqgff	198	24	20	20	CD
cord-271701-tx0lqgff	198	25	%	%	NN
cord-271701-tx0lqgff	198	26	PAGE/	PAGE/	NNP
cord-271701-tx0lqgff	198	27	used	use	VBD
cord-271701-tx0lqgff	198	28	ATP	ATP	NNP
cord-271701-tx0lqgff	198	29	and	and	CC
cord-271701-tx0lqgff	198	30	GTP	GTP	NNP
cord-271701-tx0lqgff	198	31	analogue	analogue	NN
cord-271701-tx0lqgff	198	32	ribavirin	ribavirin	NN
cord-271701-tx0lqgff	198	33	triphosphate	triphosphate	NN
cord-271701-tx0lqgff	198	34	(	(	-LRB-
cord-271701-tx0lqgff	198	35	RTP	RTP	NNP
cord-271701-tx0lqgff	198	36	)	)	-RRB-
cord-271701-tx0lqgff	198	37	(	(	-LRB-
cord-271701-tx0lqgff	198	38	24	24	CD
cord-271701-tx0lqgff	198	39	,	,	,
cord-271701-tx0lqgff	198	40	25	25	CD
cord-271701-tx0lqgff	198	41	)	)	-RRB-
cord-271701-tx0lqgff	198	42	.	.	.
cord-271701-tx0lqgff	199	1	Whether	whether	IN
cord-271701-tx0lqgff	199	2	this	this	DT
cord-271701-tx0lqgff	199	3	may	may	MD
cord-271701-tx0lqgff	199	4	offer	offer	VB
cord-271701-tx0lqgff	199	5	an	an	DT
cord-271701-tx0lqgff	199	6	explanation	explanation	NN
cord-271701-tx0lqgff	199	7	for	for	IN
cord-271701-tx0lqgff	199	8	SARS	SARS	NNP
cord-271701-tx0lqgff	199	9	-	-	HYPH
cord-271701-tx0lqgff	199	10	CoV	CoV	NNP
cord-271701-tx0lqgff	199	11	's	's	POS
cord-271701-tx0lqgff	199	12	relative	relative	JJ
cord-271701-tx0lqgff	199	13	resistance	resistance	NN
cord-271701-tx0lqgff	199	14	to	to	IN
cord-271701-tx0lqgff	199	15	this	this	DT
cord-271701-tx0lqgff	199	16	antiviral	antiviral	JJ
cord-271701-tx0lqgff	199	17	drug	drug	NN
cord-271701-tx0lqgff	199	18	(	(	-LRB-
cord-271701-tx0lqgff	199	19	26	26	CD
cord-271701-tx0lqgff	199	20	,	,	,
cord-271701-tx0lqgff	199	21	27	27	CD
cord-271701-tx0lqgff	199	22	)	)	-RRB-
cord-271701-tx0lqgff	199	23	remains	remain	VBZ
cord-271701-tx0lqgff	199	24	an	an	DT
cord-271701-tx0lqgff	199	25	open	open	JJ
cord-271701-tx0lqgff	199	26	question	question	NN
cord-271701-tx0lqgff	199	27	for	for	IN
cord-271701-tx0lqgff	199	28	future	future	JJ
cord-271701-tx0lqgff	199	29	research	research	NN
cord-271701-tx0lqgff	199	30	.	.	.
cord-271701-tx0lqgff	200	1	The	the	DT
cord-271701-tx0lqgff	200	2	primer	primer	NN
cord-271701-tx0lqgff	200	3	-	-	HYPH
cord-271701-tx0lqgff	200	4	extension	extension	NN
cord-271701-tx0lqgff	200	5	and	and	CC
cord-271701-tx0lqgff	200	6	terminal	terminal	JJ
cord-271701-tx0lqgff	200	7	transferase	transferase	NN
cord-271701-tx0lqgff	200	8	activity	activity	NN
cord-271701-tx0lqgff	200	9	documented	document	VBN
cord-271701-tx0lqgff	200	10	in	in	IN
cord-271701-tx0lqgff	200	11	Figure	figure	NN
cord-271701-tx0lqgff	200	12	4	4	CD
cord-271701-tx0lqgff	200	13	for	for	IN
cord-271701-tx0lqgff	200	14	the	the	DT
cord-271701-tx0lqgff	200	15	complex	complex	NN
cord-271701-tx0lqgff	200	16	containing	contain	VBG
cord-271701-tx0lqgff	200	17	the	the	DT
cord-271701-tx0lqgff	200	18	untagged	untagged	JJ
cord-271701-tx0lqgff	200	19	nsp8	nsp8	NN
cord-271701-tx0lqgff	200	20	was	be	VBD
cord-271701-tx0lqgff	200	21	not	not	RB
cord-271701-tx0lqgff	200	22	observed	observe	VBN
cord-271701-tx0lqgff	200	23	by	by	IN
cord-271701-tx0lqgff	200	24	Imbert	Imbert	NNP
cord-271701-tx0lqgff	200	25	et	et	NNP
cord-271701-tx0lqgff	200	26	al	al	NNP
cord-271701-tx0lqgff	200	27	.	.	.
cord-271701-tx0lqgff	201	1	(	(	-LRB-
cord-271701-tx0lqgff	201	2	12	12	CD
cord-271701-tx0lqgff	201	3	)	)	-RRB-
cord-271701-tx0lqgff	201	4	when	when	WRB
cord-271701-tx0lqgff	201	5	they	-PRON-	PRP
cord-271701-tx0lqgff	201	6	first	first	RB
cord-271701-tx0lqgff	201	7	purified	purify	VBD
cord-271701-tx0lqgff	201	8	and	and	CC
cord-271701-tx0lqgff	201	9	analysed	analyse	VBD
cord-271701-tx0lqgff	201	10	His	-PRON-	PRP$
cord-271701-tx0lqgff	201	11	-	-	HYPH
cord-271701-tx0lqgff	201	12	nsp8	nsp8	NN
cord-271701-tx0lqgff	201	13	.	.	.
cord-271701-tx0lqgff	202	1	To	to	TO
cord-271701-tx0lqgff	202	2	investigate	investigate	VB
cord-271701-tx0lqgff	202	3	whether	whether	IN
cord-271701-tx0lqgff	202	4	this	this	DT
cord-271701-tx0lqgff	202	5	difference	difference	NN
cord-271701-tx0lqgff	202	6	could	could	MD
cord-271701-tx0lqgff	202	7	be	be	VB
cord-271701-tx0lqgff	202	8	attributed	attribute	VBN
cord-271701-tx0lqgff	202	9	to	to	IN
cord-271701-tx0lqgff	202	10	complex	complex	JJ
cord-271701-tx0lqgff	202	11	formation	formation	NN
cord-271701-tx0lqgff	202	12	with	with	IN
cord-271701-tx0lqgff	202	13	nsp7	nsp7	NN
cord-271701-tx0lqgff	202	14	or	or	CC
cord-271701-tx0lqgff	202	15	the	the	DT
cord-271701-tx0lqgff	202	16	removal	removal	NN
cord-271701-tx0lqgff	202	17	of	of	IN
cord-271701-tx0lqgff	202	18	the	the	DT
cord-271701-tx0lqgff	202	19	affinity	affinity	NN
cord-271701-tx0lqgff	202	20	tag	tag	NN
cord-271701-tx0lqgff	202	21	,	,	,
cord-271701-tx0lqgff	202	22	we	-PRON-	PRP
cord-271701-tx0lqgff	202	23	performed	perform	VBD
cord-271701-tx0lqgff	202	24	the	the	DT
cord-271701-tx0lqgff	202	25	primer	primer	NN
cord-271701-tx0lqgff	202	26	extension	extension	NN
cord-271701-tx0lqgff	202	27	assay	assay	NN
cord-271701-tx0lqgff	202	28	with	with	IN
cord-271701-tx0lqgff	202	29	three	three	CD
cord-271701-tx0lqgff	202	30	different	different	JJ
cord-271701-tx0lqgff	202	31	recombinant	recombinant	JJ
cord-271701-tx0lqgff	202	32	nsp8	nsp8	JJ
cord-271701-tx0lqgff	202	33	versions	version	NNS
cord-271701-tx0lqgff	202	34	of	of	IN
cord-271701-tx0lqgff	202	35	which	which	WDT
cord-271701-tx0lqgff	202	36	the	the	DT
cord-271701-tx0lqgff	202	37	gel	gel	NN
cord-271701-tx0lqgff	202	38	filtration	filtration	NN
cord-271701-tx0lqgff	202	39	analysis	analysis	NN
cord-271701-tx0lqgff	202	40	is	be	VBZ
cord-271701-tx0lqgff	202	41	documented	document	VBN
cord-271701-tx0lqgff	202	42	in	in	IN
cord-271701-tx0lqgff	202	43	Figure	figure	NN
cord-271701-tx0lqgff	202	44	2	2	CD
cord-271701-tx0lqgff	202	45	.	.	.
cord-271701-tx0lqgff	203	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	203	2	,	,	,
cord-271701-tx0lqgff	203	3	for	for	IN
cord-271701-tx0lqgff	203	4	all	all	DT
cord-271701-tx0lqgff	203	5	three	three	CD
cord-271701-tx0lqgff	203	6	variants	variant	NNS
cord-271701-tx0lqgff	203	7	primer	primer	NN
cord-271701-tx0lqgff	203	8	-	-	HYPH
cord-271701-tx0lqgff	203	9	extension	extension	NN
cord-271701-tx0lqgff	203	10	activity	activity	NN
cord-271701-tx0lqgff	203	11	was	be	VBD
cord-271701-tx0lqgff	203	12	observed	observe	VBN
cord-271701-tx0lqgff	203	13	(	(	-LRB-
cord-271701-tx0lqgff	203	14	Figure	figure	NN
cord-271701-tx0lqgff	203	15	7A	7a	NN
cord-271701-tx0lqgff	203	16	)	)	-RRB-
cord-271701-tx0lqgff	203	17	,	,	,
cord-271701-tx0lqgff	203	18	but	but	CC
cord-271701-tx0lqgff	203	19	the	the	DT
cord-271701-tx0lqgff	203	20	activity	activity	NN
cord-271701-tx0lqgff	203	21	was	be	VBD
cord-271701-tx0lqgff	203	22	most	most	RBS
cord-271701-tx0lqgff	203	23	pronounced	pronounce	VBN
cord-271701-tx0lqgff	203	24	for	for	IN
cord-271701-tx0lqgff	203	25	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	203	26	and	and	CC
cord-271701-tx0lqgff	203	27	the	the	DT
cord-271701-tx0lqgff	203	28	untagged	untagged	JJ
cord-271701-tx0lqgff	203	29	nsp8	nsp8	NN
cord-271701-tx0lqgff	204	1	(	(	-LRB-
cord-271701-tx0lqgff	204	2	Figure	figure	NN
cord-271701-tx0lqgff	204	3	7A	7a	NN
cord-271701-tx0lqgff	204	4	)	)	-RRB-
cord-271701-tx0lqgff	204	5	.	.	.
cord-271701-tx0lqgff	205	1	To	to	TO
cord-271701-tx0lqgff	205	2	estimate	estimate	VB
cord-271701-tx0lqgff	205	3	the	the	DT
cord-271701-tx0lqgff	205	4	effect	effect	NN
cord-271701-tx0lqgff	205	5	of	of	IN
cord-271701-tx0lqgff	205	6	nsp7	nsp7	NN
cord-271701-tx0lqgff	205	7	on	on	IN
cord-271701-tx0lqgff	205	8	the	the	DT
cord-271701-tx0lqgff	205	9	nsp8-driven	nsp8-driven	NNP
cord-271701-tx0lqgff	205	10	primer	primer	NN
cord-271701-tx0lqgff	205	11	extension	extension	NN
cord-271701-tx0lqgff	205	12	activity	activity	NN
cord-271701-tx0lqgff	205	13	,	,	,
cord-271701-tx0lqgff	205	14	we	-PRON-	PRP
cord-271701-tx0lqgff	205	15	performed	perform	VBD
cord-271701-tx0lqgff	205	16	a	a	DT
cord-271701-tx0lqgff	205	17	direct	direct	JJ
cord-271701-tx0lqgff	205	18	comparison	comparison	NN
cord-271701-tx0lqgff	205	19	of	of	IN
cord-271701-tx0lqgff	205	20	the	the	DT
cord-271701-tx0lqgff	205	21	two	two	CD
cord-271701-tx0lqgff	205	22	enzyme	enzyme	NN
cord-271701-tx0lqgff	205	23	complexes	complex	NNS
cord-271701-tx0lqgff	205	24	and	and	CC
cord-271701-tx0lqgff	205	25	found	find	VBD
cord-271701-tx0lqgff	205	26	that	that	IN
cord-271701-tx0lqgff	205	27	the	the	DT
cord-271701-tx0lqgff	205	28	activity	activity	NN
cord-271701-tx0lqgff	205	29	of	of	IN
cord-271701-tx0lqgff	205	30	nsp8	nsp8	NN
cord-271701-tx0lqgff	205	31	alone	alone	JJ
cord-271701-tx0lqgff	205	32	was	be	VBD
cord-271701-tx0lqgff	205	33	>	>	XX
cord-271701-tx0lqgff	205	34	2-fold	2-fold	CD
cord-271701-tx0lqgff	205	35	lower	low	JJR
cord-271701-tx0lqgff	205	36	than	than	IN
cord-271701-tx0lqgff	205	37	when	when	WRB
cord-271701-tx0lqgff	205	38	nsp7	nsp7	JJ
cord-271701-tx0lqgff	205	39	and	and	CC
cord-271701-tx0lqgff	205	40	nsp8	nsp8	NN
cord-271701-tx0lqgff	205	41	were	be	VBD
cord-271701-tx0lqgff	205	42	present	present	JJ
cord-271701-tx0lqgff	205	43	at	at	IN
cord-271701-tx0lqgff	205	44	equal	equal	JJ
cord-271701-tx0lqgff	205	45	molarity	molarity	NN
cord-271701-tx0lqgff	205	46	in	in	IN
cord-271701-tx0lqgff	205	47	the	the	DT
cord-271701-tx0lqgff	205	48	reaction	reaction	NN
cord-271701-tx0lqgff	205	49	(	(	-LRB-
cord-271701-tx0lqgff	205	50	Figures	figure	NNS
cord-271701-tx0lqgff	205	51	4D	4d	CD
cord-271701-tx0lqgff	205	52	and	and	CC
cord-271701-tx0lqgff	205	53	7B	7B	NNP
cord-271701-tx0lqgff	205	54	)	)	-RRB-
cord-271701-tx0lqgff	205	55	.	.	.
cord-271701-tx0lqgff	206	1	A	a	DT
cord-271701-tx0lqgff	206	2	similar	similar	JJ
cord-271701-tx0lqgff	206	3	comparison	comparison	NN
cord-271701-tx0lqgff	206	4	was	be	VBD
cord-271701-tx0lqgff	206	5	performed	perform	VBN
cord-271701-tx0lqgff	206	6	for	for	IN
cord-271701-tx0lqgff	206	7	the	the	DT
cord-271701-tx0lqgff	206	8	de	de	FW
cord-271701-tx0lqgff	206	9	novo	novo	NNP
cord-271701-tx0lqgff	206	10	activity	activity	NN
cord-271701-tx0lqgff	206	11	of	of	IN
cord-271701-tx0lqgff	206	12	nsp8	nsp8	NN
cord-271701-tx0lqgff	206	13	,	,	,
cord-271701-tx0lqgff	206	14	using	use	VBG
cord-271701-tx0lqgff	206	15	the	the	DT
cord-271701-tx0lqgff	206	16	assay	assay	NN
cord-271701-tx0lqgff	206	17	published	publish	VBN
cord-271701-tx0lqgff	206	18	by	by	IN
cord-271701-tx0lqgff	206	19	Imbert	Imbert	NNP
cord-271701-tx0lqgff	206	20	et	et	NNP
cord-271701-tx0lqgff	206	21	al	al	NNP
cord-271701-tx0lqgff	206	22	.	.	.
cord-271701-tx0lqgff	207	1	(	(	-LRB-
cord-271701-tx0lqgff	207	2	12	12	CD
cord-271701-tx0lqgff	207	3	)	)	-RRB-
cord-271701-tx0lqgff	207	4	and	and	CC
cord-271701-tx0lqgff	207	5	taking	take	VBG
cord-271701-tx0lqgff	207	6	the	the	DT
cord-271701-tx0lqgff	207	7	first	first	JJ
cord-271701-tx0lqgff	207	8	dinucleotide	dinucleotide	NN
cord-271701-tx0lqgff	207	9	(	(	-LRB-
cord-271701-tx0lqgff	207	10	pppGpA	pppgpa	CD
cord-271701-tx0lqgff	207	11	)	)	-RRB-
cord-271701-tx0lqgff	207	12	product	product	NN
cord-271701-tx0lqgff	207	13	as	as	IN
cord-271701-tx0lqgff	207	14	readout	readout	NN
cord-271701-tx0lqgff	207	15	.	.	.
cord-271701-tx0lqgff	208	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	208	2	,	,	,
cord-271701-tx0lqgff	208	3	both	both	CC
cord-271701-tx0lqgff	208	4	nsp8	nsp8	NN
cord-271701-tx0lqgff	208	5	and	and	CC
cord-271701-tx0lqgff	208	6	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	208	7	+	+	SYM
cord-271701-tx0lqgff	208	8	8	8	CD
cord-271701-tx0lqgff	208	9	)	)	-RRB-
cord-271701-tx0lqgff	208	10	synthesized	synthesize	VBD
cord-271701-tx0lqgff	208	11	equal	equal	JJ
cord-271701-tx0lqgff	208	12	amounts	amount	NNS
cord-271701-tx0lqgff	208	13	of	of	IN
cord-271701-tx0lqgff	208	14	the	the	DT
cord-271701-tx0lqgff	208	15	pppGpA	pppgpa	SYM
cord-271701-tx0lqgff	208	16	dinucleotide	dinucleotide	NN
cord-271701-tx0lqgff	208	17	(	(	-LRB-
cord-271701-tx0lqgff	208	18	Figure	Figure	NNP
cord-271701-tx0lqgff	208	19	7C	7C	NNP
cord-271701-tx0lqgff	208	20	)	)	-RRB-
cord-271701-tx0lqgff	208	21	,	,	,
cord-271701-tx0lqgff	208	22	suggesting	suggest	VBG
cord-271701-tx0lqgff	208	23	that	that	IN
cord-271701-tx0lqgff	208	24	the	the	DT
cord-271701-tx0lqgff	208	25	effect	effect	NN
cord-271701-tx0lqgff	208	26	of	of	IN
cord-271701-tx0lqgff	208	27	nsp8	nsp8	NN
cord-271701-tx0lqgff	208	28	is	be	VBZ
cord-271701-tx0lqgff	208	29	limited	limit	VBN
cord-271701-tx0lqgff	208	30	to	to	IN
cord-271701-tx0lqgff	208	31	the	the	DT
cord-271701-tx0lqgff	208	32	primer	primer	NN
cord-271701-tx0lqgff	208	33	-	-	HYPH
cord-271701-tx0lqgff	208	34	extension	extension	NN
cord-271701-tx0lqgff	208	35	activity	activity	NN
cord-271701-tx0lqgff	208	36	of	of	IN
cord-271701-tx0lqgff	208	37	nsp8	nsp8	NN
cord-271701-tx0lqgff	208	38	.	.	.
cord-271701-tx0lqgff	209	1	In	in	IN
cord-271701-tx0lqgff	209	2	addition	addition	NN
cord-271701-tx0lqgff	209	3	,	,	,
cord-271701-tx0lqgff	209	4	we	-PRON-	PRP
cord-271701-tx0lqgff	209	5	observed	observe	VBD
cord-271701-tx0lqgff	209	6	that	that	IN
cord-271701-tx0lqgff	209	7	the	the	DT
cord-271701-tx0lqgff	209	8	de	de	FW
cord-271701-tx0lqgff	209	9	novo	novo	NNP
cord-271701-tx0lqgff	209	10	initiation	initiation	NN
cord-271701-tx0lqgff	209	11	activity	activity	NN
cord-271701-tx0lqgff	209	12	of	of	IN
cord-271701-tx0lqgff	209	13	nsp8	nsp8	NN
cord-271701-tx0lqgff	209	14	was	be	VBD
cord-271701-tx0lqgff	209	15	$	$	$
cord-271701-tx0lqgff	209	16	2-fold	2-fold	CD
cord-271701-tx0lqgff	209	17	higher	high	JJR
cord-271701-tx0lqgff	209	18	than	than	IN
cord-271701-tx0lqgff	209	19	that	that	DT
cord-271701-tx0lqgff	209	20	of	of	IN
cord-271701-tx0lqgff	209	21	His	-PRON-	PRP$
cord-271701-tx0lqgff	209	22	-	-	HYPH
cord-271701-tx0lqgff	209	23	nsp8	nsp8	NN
cord-271701-tx0lqgff	209	24	(	(	-LRB-
cord-271701-tx0lqgff	209	25	Figure	figure	NN
cord-271701-tx0lqgff	209	26	7D	7D	NNP
cord-271701-tx0lqgff	209	27	)	)	-RRB-
cord-271701-tx0lqgff	209	28	.	.	.
cord-271701-tx0lqgff	210	1	Our	-PRON-	PRP$
cord-271701-tx0lqgff	210	2	comparative	comparative	JJ
cord-271701-tx0lqgff	210	3	study	study	NN
cord-271701-tx0lqgff	210	4	revealed	reveal	VBD
cord-271701-tx0lqgff	210	5	that	that	IN
cord-271701-tx0lqgff	210	6	the	the	DT
cord-271701-tx0lqgff	210	7	N	n	CD
cord-271701-tx0lqgff	210	8	-	-	HYPH
cord-271701-tx0lqgff	210	9	terminal	terminal	NN
cord-271701-tx0lqgff	210	10	His	-PRON-	PRP$
cord-271701-tx0lqgff	210	11	6	6	CD
cord-271701-tx0lqgff	210	12	-tag	-tag	:
cord-271701-tx0lqgff	210	13	of	of	IN
cord-271701-tx0lqgff	210	14	His	-PRON-	PRP$
cord-271701-tx0lqgff	210	15	-	-	HYPH
cord-271701-tx0lqgff	210	16	nsp8	nsp8	NN
cord-271701-tx0lqgff	210	17	greatly	greatly	RB
cord-271701-tx0lqgff	210	18	influences	influence	VBZ
cord-271701-tx0lqgff	210	19	the	the	DT
cord-271701-tx0lqgff	210	20	primerextension	primerextension	NN
cord-271701-tx0lqgff	210	21	activity	activity	NN
cord-271701-tx0lqgff	210	22	of	of	IN
cord-271701-tx0lqgff	210	23	nsp8	nsp8	NN
cord-271701-tx0lqgff	210	24	(	(	-LRB-
cord-271701-tx0lqgff	210	25	Figure	figure	NN
cord-271701-tx0lqgff	210	26	7A	7a	NN
cord-271701-tx0lqgff	210	27	)	)	-RRB-
cord-271701-tx0lqgff	210	28	,	,	,
cord-271701-tx0lqgff	210	29	its	-PRON-	PRP$
cord-271701-tx0lqgff	210	30	multimerization	multimerization	NN
cord-271701-tx0lqgff	210	31	profile	profile	NN
cord-271701-tx0lqgff	210	32	and	and	CC
cord-271701-tx0lqgff	210	33	its	-PRON-	PRP$
cord-271701-tx0lqgff	210	34	association	association	NN
cord-271701-tx0lqgff	210	35	with	with	IN
cord-271701-tx0lqgff	210	36	nsp7	nsp7	NNP
cord-271701-tx0lqgff	210	37	(	(	-LRB-
cord-271701-tx0lqgff	210	38	Figure	figure	NN
cord-271701-tx0lqgff	210	39	2	2	CD
cord-271701-tx0lqgff	210	40	)	)	-RRB-
cord-271701-tx0lqgff	210	41	.	.	.
cord-271701-tx0lqgff	211	1	To	to	TO
cord-271701-tx0lqgff	211	2	test	test	VB
cord-271701-tx0lqgff	211	3	if	if	IN
cord-271701-tx0lqgff	211	4	this	this	DT
cord-271701-tx0lqgff	211	5	inhibitory	inhibitory	JJ
cord-271701-tx0lqgff	211	6	effect	effect	NN
cord-271701-tx0lqgff	211	7	was	be	VBD
cord-271701-tx0lqgff	211	8	His	-PRON-	PRP$
cord-271701-tx0lqgff	211	9	6	6	CD
cord-271701-tx0lqgff	211	10	-tag	-tag	RBS
cord-271701-tx0lqgff	211	11	specific	specific	JJ
cord-271701-tx0lqgff	211	12	,	,	,
cord-271701-tx0lqgff	211	13	we	-PRON-	PRP
cord-271701-tx0lqgff	211	14	assessed	assess	VBD
cord-271701-tx0lqgff	211	15	the	the	DT
cord-271701-tx0lqgff	211	16	activity	activity	NN
cord-271701-tx0lqgff	211	17	of	of	IN
cord-271701-tx0lqgff	211	18	a	a	DT
cord-271701-tx0lqgff	211	19	ub	ub	NNP
cord-271701-tx0lqgff	211	20	-	-	HYPH
cord-271701-tx0lqgff	211	21	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	211	22	fusion	fusion	NN
cord-271701-tx0lqgff	211	23	protein	protein	NN
cord-271701-tx0lqgff	211	24	.	.	.
cord-271701-tx0lqgff	212	1	At	at	IN
cord-271701-tx0lqgff	212	2	the	the	DT
cord-271701-tx0lqgff	212	3	same	same	JJ
cord-271701-tx0lqgff	212	4	time	time	NN
cord-271701-tx0lqgff	212	5	,	,	,
cord-271701-tx0lqgff	212	6	control	control	NN
cord-271701-tx0lqgff	212	7	reactions	reaction	NNS
cord-271701-tx0lqgff	212	8	were	be	VBD
cord-271701-tx0lqgff	212	9	performed	perform	VBN
cord-271701-tx0lqgff	212	10	in	in	IN
cord-271701-tx0lqgff	212	11	which	which	WDT
cord-271701-tx0lqgff	212	12	we	-PRON-	PRP
cord-271701-tx0lqgff	212	13	(	(	-LRB-
cord-271701-tx0lqgff	212	14	i	i	NN
cord-271701-tx0lqgff	212	15	)	)	-RRB-
cord-271701-tx0lqgff	212	16	followed	follow	VBD
cord-271701-tx0lqgff	212	17	the	the	DT
cord-271701-tx0lqgff	212	18	activity	activity	NN
cord-271701-tx0lqgff	212	19	of	of	IN
cord-271701-tx0lqgff	212	20	this	this	DT
cord-271701-tx0lqgff	212	21	protein	protein	NN
cord-271701-tx0lqgff	212	22	as	as	IN
cord-271701-tx0lqgff	212	23	it	-PRON-	PRP
cord-271701-tx0lqgff	212	24	was	be	VBD
cord-271701-tx0lqgff	212	25	being	be	VBG
cord-271701-tx0lqgff	212	26	processed	process	VBN
cord-271701-tx0lqgff	212	27	by	by	IN
cord-271701-tx0lqgff	212	28	a	a	DT
cord-271701-tx0lqgff	212	29	recombinant	recombinant	JJ
cord-271701-tx0lqgff	212	30	form	form	NN
cord-271701-tx0lqgff	212	31	of	of	IN
cord-271701-tx0lqgff	212	32	the	the	DT
cord-271701-tx0lqgff	212	33	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	212	34	-	-	HYPH
cord-271701-tx0lqgff	212	35	cleaving	cleave	VBG
cord-271701-tx0lqgff	212	36	nsp2	nsp2	JJR
cord-271701-tx0lqgff	212	37	protease	protease	NN
cord-271701-tx0lqgff	212	38	of	of	IN
cord-271701-tx0lqgff	212	39	equine	equine	JJ
cord-271701-tx0lqgff	212	40	arteritis	arteritis	NN
cord-271701-tx0lqgff	212	41	virus	virus	NN
cord-271701-tx0lqgff	212	42	(	(	-LRB-
cord-271701-tx0lqgff	212	43	28	28	CD
cord-271701-tx0lqgff	212	44	)	)	-RRB-
cord-271701-tx0lqgff	212	45	or	or	CC
cord-271701-tx0lqgff	212	46	(	(	-LRB-
cord-271701-tx0lqgff	212	47	ii	ii	NN
cord-271701-tx0lqgff	212	48	)	)	-RRB-
cord-271701-tx0lqgff	212	49	monitored	monitor	VBD
cord-271701-tx0lqgff	212	50	the	the	DT
cord-271701-tx0lqgff	212	51	activity	activity	NN
cord-271701-tx0lqgff	212	52	of	of	IN
cord-271701-tx0lqgff	212	53	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	212	54	.	.	.
cord-271701-tx0lqgff	213	1	As	as	IN
cord-271701-tx0lqgff	213	2	shown	show	VBN
cord-271701-tx0lqgff	213	3	in	in	IN
cord-271701-tx0lqgff	213	4	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	213	5	Figure	Figure	NNP
cord-271701-tx0lqgff	213	6	S3	S3	NNP
cord-271701-tx0lqgff	213	7	,	,	,
cord-271701-tx0lqgff	213	8	the	the	DT
cord-271701-tx0lqgff	213	9	presence	presence	NN
cord-271701-tx0lqgff	213	10	of	of	IN
cord-271701-tx0lqgff	213	11	the	the	DT
cord-271701-tx0lqgff	213	12	ub	ub	NNP
cord-271701-tx0lqgff	213	13	-	-	HYPH
cord-271701-tx0lqgff	213	14	tag	tag	NN
cord-271701-tx0lqgff	213	15	decreased	decrease	VBD
cord-271701-tx0lqgff	213	16	nsp8	nsp8	JJ
cord-271701-tx0lqgff	213	17	activity	activity	NN
cord-271701-tx0lqgff	213	18	to	to	IN
cord-271701-tx0lqgff	213	19	a	a	DT
cord-271701-tx0lqgff	213	20	level	level	NN
cord-271701-tx0lqgff	213	21	that	that	WDT
cord-271701-tx0lqgff	213	22	was	be	VBD
cord-271701-tx0lqgff	213	23	comparable	comparable	JJ
cord-271701-tx0lqgff	213	24	to	to	IN
cord-271701-tx0lqgff	213	25	that	that	DT
cord-271701-tx0lqgff	213	26	of	of	IN
cord-271701-tx0lqgff	213	27	N	n	NN
cord-271701-tx0lqgff	213	28	-	-	HYPH
cord-271701-tx0lqgff	213	29	terminally	terminally	RB
cord-271701-tx0lqgff	213	30	His	-PRON-	PRP$
cord-271701-tx0lqgff	213	31	6	6	CD
cord-271701-tx0lqgff	213	32	-tagged	-tagged	CD
cord-271701-tx0lqgff	213	33	nsp8	nsp8	NN
cord-271701-tx0lqgff	213	34	.	.	.
cord-271701-tx0lqgff	214	1	Upon	upon	IN
cord-271701-tx0lqgff	214	2	cleavage	cleavage	NN
cord-271701-tx0lqgff	214	3	by	by	IN
cord-271701-tx0lqgff	214	4	EAV	EAV	NNP
cord-271701-tx0lqgff	214	5	nsp2	nsp2	NNP
cord-271701-tx0lqgff	214	6	,	,	,
cord-271701-tx0lqgff	214	7	however	however	RB
cord-271701-tx0lqgff	214	8	,	,	,
cord-271701-tx0lqgff	214	9	a	a	DT
cord-271701-tx0lqgff	214	10	partial	partial	JJ
cord-271701-tx0lqgff	214	11	recovery	recovery	NN
cord-271701-tx0lqgff	214	12	of	of	IN
cord-271701-tx0lqgff	214	13	the	the	DT
cord-271701-tx0lqgff	214	14	primer	primer	NN
cord-271701-tx0lqgff	214	15	extension	extension	NN
cord-271701-tx0lqgff	214	16	activity	activity	NN
cord-271701-tx0lqgff	214	17	was	be	VBD
cord-271701-tx0lqgff	214	18	observed	observe	VBN
cord-271701-tx0lqgff	214	19	(	(	-LRB-
cord-271701-tx0lqgff	214	20	Supplementary	supplementary	JJ
cord-271701-tx0lqgff	214	21	Figure	Figure	NNP
cord-271701-tx0lqgff	214	22	S3	S3	NNP
cord-271701-tx0lqgff	214	23	)	)	-RRB-
cord-271701-tx0lqgff	214	24	.	.	.
cord-271701-tx0lqgff	215	1	Unfortunately	unfortunately	RB
cord-271701-tx0lqgff	215	2	,	,	,
cord-271701-tx0lqgff	215	3	we	-PRON-	PRP
cord-271701-tx0lqgff	215	4	were	be	VBD
cord-271701-tx0lqgff	215	5	not	not	RB
cord-271701-tx0lqgff	215	6	able	able	JJ
cord-271701-tx0lqgff	215	7	to	to	TO
cord-271701-tx0lqgff	215	8	perform	perform	VB
cord-271701-tx0lqgff	215	9	the	the	DT
cord-271701-tx0lqgff	215	10	same	same	JJ
cord-271701-tx0lqgff	215	11	experiment	experiment	NN
cord-271701-tx0lqgff	215	12	with	with	IN
cord-271701-tx0lqgff	215	13	purified	purify	VBN
cord-271701-tx0lqgff	215	14	ub	ub	NNP
cord-271701-tx0lqgff	215	15	-	-	HYPH
cord-271701-tx0lqgff	215	16	nsp8	nsp8	NNP
cord-271701-tx0lqgff	215	17	,	,	,
cord-271701-tx0lqgff	215	18	since	since	IN
cord-271701-tx0lqgff	215	19	our	-PRON-	PRP$
cord-271701-tx0lqgff	215	20	recombinant	recombinant	JJ
cord-271701-tx0lqgff	215	21	nsp5	nsp5	NN
cord-271701-tx0lqgff	215	22	removed	remove	VBN
cord-271701-tx0lqgff	215	23	the	the	DT
cord-271701-tx0lqgff	215	24	N	n	CD
cord-271701-tx0lqgff	215	25	-	-	HYPH
cord-271701-tx0lqgff	215	26	terminal	terminal	JJ
cord-271701-tx0lqgff	215	27	ub	ub	NNP
cord-271701-tx0lqgff	215	28	-	-	HYPH
cord-271701-tx0lqgff	215	29	tag	tag	NN
cord-271701-tx0lqgff	215	30	with	with	IN
cord-271701-tx0lqgff	215	31	similar	similar	JJ
cord-271701-tx0lqgff	215	32	efficiency	efficiency	NN
cord-271701-tx0lqgff	215	33	as	as	IN
cord-271701-tx0lqgff	215	34	the	the	DT
cord-271701-tx0lqgff	215	35	C	C	NNP
cord-271701-tx0lqgff	215	36	-	-	HYPH
cord-271701-tx0lqgff	215	37	terminal	terminal	NN
cord-271701-tx0lqgff	216	1	His	-PRON-	PRP$
cord-271701-tx0lqgff	216	2	6	6	CD
cord-271701-tx0lqgff	216	3	-tag	-tag	:
cord-271701-tx0lqgff	216	4	(	(	-LRB-
cord-271701-tx0lqgff	216	5	Supplementary	supplementary	JJ
cord-271701-tx0lqgff	216	6	Figure	Figure	NNP
cord-271701-tx0lqgff	216	7	S4	S4	NNP
cord-271701-tx0lqgff	216	8	)	)	-RRB-
cord-271701-tx0lqgff	216	9	.	.	.
cord-271701-tx0lqgff	217	1	Extrapolating	extrapolate	VBG
cord-271701-tx0lqgff	217	2	to	to	IN
cord-271701-tx0lqgff	217	3	the	the	DT
cord-271701-tx0lqgff	217	4	situation	situation	NN
cord-271701-tx0lqgff	217	5	in	in	IN
cord-271701-tx0lqgff	217	6	the	the	DT
cord-271701-tx0lqgff	217	7	viral	viral	JJ
cord-271701-tx0lqgff	217	8	pp1a	pp1a	NNP
cord-271701-tx0lqgff	217	9	and	and	CC
cord-271701-tx0lqgff	217	10	pp1ab	pp1ab	NNP
cord-271701-tx0lqgff	217	11	precursor	precursor	NN
cord-271701-tx0lqgff	217	12	polyproteins	polyprotein	NNS
cord-271701-tx0lqgff	217	13	,	,	,
cord-271701-tx0lqgff	217	14	in	in	IN
cord-271701-tx0lqgff	217	15	which	which	WDT
cord-271701-tx0lqgff	217	16	the	the	DT
cord-271701-tx0lqgff	217	17	nsp8	nsp8	NNP
cord-271701-tx0lqgff	217	18	N	N	NNP
cord-271701-tx0lqgff	217	19	-	-	HYPH
cord-271701-tx0lqgff	217	20	terminus	terminus	NN
cord-271701-tx0lqgff	217	21	is	be	VBZ
cord-271701-tx0lqgff	217	22	initially	initially	RB
cord-271701-tx0lqgff	217	23	fused	fuse	VBN
cord-271701-tx0lqgff	217	24	to	to	IN
cord-271701-tx0lqgff	217	25	nsp7	nsp7	NNP
cord-271701-tx0lqgff	217	26	(	(	-LRB-
cord-271701-tx0lqgff	217	27	Figure	figure	NN
cord-271701-tx0lqgff	217	28	1A	1a	NN
cord-271701-tx0lqgff	217	29	)	)	-RRB-
cord-271701-tx0lqgff	217	30	,	,	,
cord-271701-tx0lqgff	217	31	our	-PRON-	PRP$
cord-271701-tx0lqgff	217	32	observations	observation	NNS
cord-271701-tx0lqgff	217	33	suggested	suggest	VBD
cord-271701-tx0lqgff	217	34	that	that	IN
cord-271701-tx0lqgff	217	35	nsp8	nsp8	NN
cord-271701-tx0lqgff	217	36	may	may	MD
cord-271701-tx0lqgff	217	37	thus	thus	RB
cord-271701-tx0lqgff	217	38	be	be	VB
cord-271701-tx0lqgff	217	39	inactive	inactive	JJ
cord-271701-tx0lqgff	217	40	in	in	IN
cord-271701-tx0lqgff	217	41	the	the	DT
cord-271701-tx0lqgff	217	42	polyprotein	polyprotein	JJ
cord-271701-tx0lqgff	217	43	context	context	NN
cord-271701-tx0lqgff	217	44	.	.	.
cord-271701-tx0lqgff	218	1	This	this	DT
cord-271701-tx0lqgff	218	2	would	would	MD
cord-271701-tx0lqgff	218	3	constitute	constitute	VB
cord-271701-tx0lqgff	218	4	a	a	DT
cord-271701-tx0lqgff	218	5	form	form	NN
cord-271701-tx0lqgff	218	6	of	of	IN
cord-271701-tx0lqgff	218	7	regulation	regulation	NN
cord-271701-tx0lqgff	218	8	of	of	IN
cord-271701-tx0lqgff	218	9	viral	viral	JJ
cord-271701-tx0lqgff	218	10	enzyme	enzyme	NN
cord-271701-tx0lqgff	218	11	activity	activity	NN
cord-271701-tx0lqgff	218	12	that	that	WDT
cord-271701-tx0lqgff	218	13	is	be	VBZ
cord-271701-tx0lqgff	218	14	not	not	RB
cord-271701-tx0lqgff	218	15	without	without	IN
cord-271701-tx0lqgff	218	16	Figure	figure	NN
cord-271701-tx0lqgff	218	17	6A	6a	CD
cord-271701-tx0lqgff	218	18	,	,	,
cord-271701-tx0lqgff	218	19	presented	present	VBN
cord-271701-tx0lqgff	218	20	as	as	IN
cord-271701-tx0lqgff	218	21	the	the	DT
cord-271701-tx0lqgff	218	22	amount	amount	NN
cord-271701-tx0lqgff	218	23	of	of	IN
cord-271701-tx0lqgff	218	24	NTP	NTP	NNP
cord-271701-tx0lqgff	218	25	incorporated	incorporate	VBN
cord-271701-tx0lqgff	218	26	per	per	IN
cord-271701-tx0lqgff	218	27	mM	mm	CD
cord-271701-tx0lqgff	218	28	nsp8	nsp8	JJ
cord-271701-tx0lqgff	218	29	monomer	monomer	NN
cord-271701-tx0lqgff	218	30	.	.	.
cord-271701-tx0lqgff	219	1	Error	error	NN
cord-271701-tx0lqgff	219	2	bars	bar	NNS
cord-271701-tx0lqgff	219	3	represent	represent	VBP
cord-271701-tx0lqgff	219	4	standard	standard	JJ
cord-271701-tx0lqgff	219	5	deviations	deviation	NNS
cord-271701-tx0lqgff	219	6	(	(	-LRB-
cord-271701-tx0lqgff	219	7	n	n	NN
cord-271701-tx0lqgff	219	8	=	=	SYM
cord-271701-tx0lqgff	219	9	3	3	CD
cord-271701-tx0lqgff	219	10	)	)	-RRB-
cord-271701-tx0lqgff	219	11	.	.	.
cord-271701-tx0lqgff	220	1	(	(	-LRB-
cord-271701-tx0lqgff	220	2	C	C	NNP
cord-271701-tx0lqgff	220	3	)	)	-RRB-
cord-271701-tx0lqgff	221	1	The	the	DT
cord-271701-tx0lqgff	221	2	influence	influence	NN
cord-271701-tx0lqgff	221	3	of	of	IN
cord-271701-tx0lqgff	221	4	the	the	DT
cord-271701-tx0lqgff	221	5	pH	pH	NNP
cord-271701-tx0lqgff	221	6	on	on	IN
cord-271701-tx0lqgff	221	7	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	221	8	+	+	SYM
cord-271701-tx0lqgff	221	9	8	8	CD
cord-271701-tx0lqgff	221	10	)	)	-RRB-
cord-271701-tx0lqgff	221	11	activity	activity	NN
cord-271701-tx0lqgff	221	12	was	be	VBD
cord-271701-tx0lqgff	221	13	tested	test	VBN
cord-271701-tx0lqgff	221	14	for	for	IN
cord-271701-tx0lqgff	221	15	a	a	DT
cord-271701-tx0lqgff	221	16	pH	pH	NNP
cord-271701-tx0lqgff	221	17	range	range	NN
cord-271701-tx0lqgff	221	18	of	of	IN
cord-271701-tx0lqgff	221	19	6	6	CD
cord-271701-tx0lqgff	221	20	-	-	SYM
cord-271701-tx0lqgff	221	21	11	11	CD
cord-271701-tx0lqgff	221	22	.	.	.
cord-271701-tx0lqgff	222	1	A	a	DT
cord-271701-tx0lqgff	222	2	clear	clear	JJ
cord-271701-tx0lqgff	222	3	optimum	optimum	NN
cord-271701-tx0lqgff	222	4	was	be	VBD
cord-271701-tx0lqgff	222	5	observed	observe	VBN
cord-271701-tx0lqgff	222	6	around	around	IN
cord-271701-tx0lqgff	222	7	9.5	9.5	CD
cord-271701-tx0lqgff	222	8	.	.	.
cord-271701-tx0lqgff	223	1	(	(	-LRB-
cord-271701-tx0lqgff	223	2	D	D	NNP
cord-271701-tx0lqgff	223	3	)	)	-RRB-
cord-271701-tx0lqgff	223	4	Quantification	quantification	NN
cord-271701-tx0lqgff	223	5	of	of	IN
cord-271701-tx0lqgff	223	6	the	the	DT
cord-271701-tx0lqgff	223	7	results	result	NNS
cord-271701-tx0lqgff	223	8	in	in	IN
cord-271701-tx0lqgff	223	9	Figure	figure	NN
cord-271701-tx0lqgff	223	10	6C	6c	NN
cord-271701-tx0lqgff	223	11	,	,	,
cord-271701-tx0lqgff	223	12	presented	present	VBN
cord-271701-tx0lqgff	223	13	as	as	IN
cord-271701-tx0lqgff	223	14	the	the	DT
cord-271701-tx0lqgff	223	15	amount	amount	NN
cord-271701-tx0lqgff	223	16	of	of	IN
cord-271701-tx0lqgff	223	17	NTP	NTP	NNP
cord-271701-tx0lqgff	223	18	incorporated	incorporate	VBN
cord-271701-tx0lqgff	223	19	per	per	IN
cord-271701-tx0lqgff	223	20	mM	mm	CD
cord-271701-tx0lqgff	223	21	nsp8	nsp8	JJ
cord-271701-tx0lqgff	223	22	monomer	monomer	NN
cord-271701-tx0lqgff	223	23	.	.	.
cord-271701-tx0lqgff	224	1	Error	error	NN
cord-271701-tx0lqgff	224	2	bars	bar	NNS
cord-271701-tx0lqgff	224	3	indicate	indicate	VBP
cord-271701-tx0lqgff	224	4	standard	standard	JJ
cord-271701-tx0lqgff	224	5	deviations	deviation	NNS
cord-271701-tx0lqgff	224	6	(	(	-LRB-
cord-271701-tx0lqgff	224	7	n	n	NN
cord-271701-tx0lqgff	224	8	=	=	SYM
cord-271701-tx0lqgff	224	9	3	3	CD
cord-271701-tx0lqgff	224	10	)	)	-RRB-
cord-271701-tx0lqgff	224	11	.	.	.
cord-271701-tx0lqgff	225	1	(	(	-LRB-
cord-271701-tx0lqgff	225	2	E	e	NN
cord-271701-tx0lqgff	225	3	)	)	-RRB-
cord-271701-tx0lqgff	226	1	Schematic	schematic	JJ
cord-271701-tx0lqgff	226	2	presentation	presentation	NN
cord-271701-tx0lqgff	226	3	of	of	IN
cord-271701-tx0lqgff	226	4	the	the	DT
cord-271701-tx0lqgff	226	5	pulse	pulse	NN
cord-271701-tx0lqgff	226	6	-	-	HYPH
cord-271701-tx0lqgff	226	7	chase	chase	NN
cord-271701-tx0lqgff	226	8	experiment	experiment	NN
cord-271701-tx0lqgff	226	9	that	that	WDT
cord-271701-tx0lqgff	226	10	was	be	VBD
cord-271701-tx0lqgff	226	11	used	use	VBN
cord-271701-tx0lqgff	226	12	to	to	TO
cord-271701-tx0lqgff	226	13	test	test	VB
cord-271701-tx0lqgff	226	14	the	the	DT
cord-271701-tx0lqgff	226	15	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	226	16	+	+	SYM
cord-271701-tx0lqgff	226	17	8	8	CD
cord-271701-tx0lqgff	226	18	)	)	-RRB-
cord-271701-tx0lqgff	226	19	nucleotide	nucleotide	JJ
cord-271701-tx0lqgff	226	20	incorporation	incorporation	NN
cord-271701-tx0lqgff	226	21	specificity	specificity	NN
cord-271701-tx0lqgff	226	22	on	on	IN
cord-271701-tx0lqgff	226	23	a	a	DT
cord-271701-tx0lqgff	226	24	primed	primed	JJ
cord-271701-tx0lqgff	226	25	poly(U	poly(u	NN
cord-271701-tx0lqgff	226	26	)	)	-RRB-
cord-271701-tx0lqgff	226	27	template	template	NN
cord-271701-tx0lqgff	226	28	(	(	-LRB-
cord-271701-tx0lqgff	226	29	see	see	VB
cord-271701-tx0lqgff	226	30	Table	table	NN
cord-271701-tx0lqgff	226	31	1	1	CD
cord-271701-tx0lqgff	226	32	)	)	-RRB-
cord-271701-tx0lqgff	226	33	.	.	.
cord-271701-tx0lqgff	227	1	The	the	DT
cord-271701-tx0lqgff	227	2	reactions	reaction	NNS
cord-271701-tx0lqgff	227	3	were	be	VBD
cord-271701-tx0lqgff	227	4	initiated	initiate	VBN
cord-271701-tx0lqgff	227	5	with	with	IN
cord-271701-tx0lqgff	227	6	a	a	DT
cord-271701-tx0lqgff	227	7	limiting	limit	VBG
cord-271701-tx0lqgff	227	8	concentration	concentration	NN
cord-271701-tx0lqgff	227	9	of	of	IN
cord-271701-tx0lqgff	227	10	[	[	-LRB-
cord-271701-tx0lqgff	227	11	a-32	a-32	NNP
cord-271701-tx0lqgff	227	12	P]ATP	P]ATP	NNP
cord-271701-tx0lqgff	227	13	to	to	TO
cord-271701-tx0lqgff	227	14	allow	allow	VB
cord-271701-tx0lqgff	227	15	the	the	DT
cord-271701-tx0lqgff	227	16	formation	formation	NN
cord-271701-tx0lqgff	227	17	of	of	IN
cord-271701-tx0lqgff	227	18	a	a	DT
cord-271701-tx0lqgff	227	19	stable	stable	JJ
cord-271701-tx0lqgff	227	20	polymerase	polymerase	NN
cord-271701-tx0lqgff	227	21	-	-	HYPH
cord-271701-tx0lqgff	227	22	template	template	NN
cord-271701-tx0lqgff	227	23	complex	complex	NN
cord-271701-tx0lqgff	227	24	.	.	.
cord-271701-tx0lqgff	228	1	Unlabelled	unlabelled	JJ
cord-271701-tx0lqgff	228	2	nucleotides	nucleotide	NNS
cord-271701-tx0lqgff	228	3	were	be	VBD
cord-271701-tx0lqgff	228	4	used	use	VBN
cord-271701-tx0lqgff	228	5	at	at	IN
cord-271701-tx0lqgff	228	6	a	a	DT
cord-271701-tx0lqgff	228	7	final	final	JJ
cord-271701-tx0lqgff	228	8	concentration	concentration	NN
cord-271701-tx0lqgff	228	9	of	of	IN
cord-271701-tx0lqgff	228	10	50	50	CD
cord-271701-tx0lqgff	228	11	mM.	mM.	NNS
cord-271701-tx0lqgff	228	12	(	(	-LRB-
cord-271701-tx0lqgff	228	13	F	F	NNP
cord-271701-tx0lqgff	228	14	)	)	-RRB-
cord-271701-tx0lqgff	228	15	SARS	SARS	NNP
cord-271701-tx0lqgff	228	16	-	-	HYPH
cord-271701-tx0lqgff	228	17	CoV	CoV	NNP
cord-271701-tx0lqgff	228	18	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	228	19	+	+	SYM
cord-271701-tx0lqgff	228	20	8	8	CD
cord-271701-tx0lqgff	228	21	)	)	-RRB-
cord-271701-tx0lqgff	228	22	allowed	allow	VBN
cord-271701-tx0lqgff	228	23	only	only	RB
cord-271701-tx0lqgff	228	24	limited	limit	VBN
cord-271701-tx0lqgff	228	25	transversional	transversional	JJ
cord-271701-tx0lqgff	228	26	and	and	CC
cord-271701-tx0lqgff	228	27	transitional	transitional	JJ
cord-271701-tx0lqgff	228	28	mutations	mutation	NNS
cord-271701-tx0lqgff	228	29	.	.	.
cord-271701-tx0lqgff	229	1	Use	use	NN
cord-271701-tx0lqgff	229	2	of	of	IN
cord-271701-tx0lqgff	229	3	manganese	manganese	NN
cord-271701-tx0lqgff	229	4	ions	ion	NNS
cord-271701-tx0lqgff	229	5	as	as	IN
cord-271701-tx0lqgff	229	6	cofactor	cofactor	NN
cord-271701-tx0lqgff	229	7	for	for	IN
cord-271701-tx0lqgff	229	8	polymerase	polymerase	NN
cord-271701-tx0lqgff	229	9	activity	activity	NN
cord-271701-tx0lqgff	229	10	resulted	result	VBD
cord-271701-tx0lqgff	229	11	in	in	IN
cord-271701-tx0lqgff	229	12	a	a	DT
cord-271701-tx0lqgff	229	13	minor	minor	JJ
cord-271701-tx0lqgff	229	14	,	,	,
cord-271701-tx0lqgff	229	15	though	though	IN
cord-271701-tx0lqgff	229	16	noticeable	noticeable	JJ
cord-271701-tx0lqgff	229	17	loss	loss	NN
cord-271701-tx0lqgff	229	18	of	of	IN
cord-271701-tx0lqgff	229	19	fidelity	fidelity	NN
cord-271701-tx0lqgff	229	20	.	.	.
cord-271701-tx0lqgff	230	1	Lane	lane	NN
cord-271701-tx0lqgff	230	2	1	1	CD
cord-271701-tx0lqgff	230	3	represents	represent	VBZ
cord-271701-tx0lqgff	230	4	the	the	DT
cord-271701-tx0lqgff	230	5	input	input	NN
cord-271701-tx0lqgff	230	6	signal	signal	NN
cord-271701-tx0lqgff	230	7	to	to	TO
cord-271701-tx0lqgff	230	8	which	which	WDT
cord-271701-tx0lqgff	230	9	no	no	DT
cord-271701-tx0lqgff	230	10	unlabelled	unlabelled	JJ
cord-271701-tx0lqgff	230	11	nucleotides	nucleotide	NNS
cord-271701-tx0lqgff	230	12	were	be	VBD
cord-271701-tx0lqgff	230	13	added	add	VBN
cord-271701-tx0lqgff	230	14	.	.	.
cord-271701-tx0lqgff	231	1	Nucleoside	nucleoside	NN
cord-271701-tx0lqgff	231	2	triphosphates	triphosphate	NNS
cord-271701-tx0lqgff	231	3	are	be	VBP
cord-271701-tx0lqgff	231	4	abbreviated	abbreviate	VBN
cord-271701-tx0lqgff	231	5	to	to	IN
cord-271701-tx0lqgff	231	6	single	single	JJ
cord-271701-tx0lqgff	231	7	letters	letter	NNS
cord-271701-tx0lqgff	231	8	(	(	-LRB-
cord-271701-tx0lqgff	231	9	i.e.	i.e.	FW
cord-271701-tx0lqgff	231	10	A	a	NN
cord-271701-tx0lqgff	231	11	for	for	IN
cord-271701-tx0lqgff	231	12	ATP	ATP	NNP
cord-271701-tx0lqgff	231	13	,	,	,
cord-271701-tx0lqgff	231	14	G	G	NNP
cord-271701-tx0lqgff	231	15	for	for	IN
cord-271701-tx0lqgff	231	16	GTP	GTP	NNP
cord-271701-tx0lqgff	231	17	,	,	,
cord-271701-tx0lqgff	231	18	U	U	NNP
cord-271701-tx0lqgff	231	19	for	for	IN
cord-271701-tx0lqgff	231	20	UTP	UTP	NNP
cord-271701-tx0lqgff	231	21	,	,	,
cord-271701-tx0lqgff	231	22	C	C	NNP
cord-271701-tx0lqgff	231	23	for	for	IN
cord-271701-tx0lqgff	231	24	CTP	CTP	NNP
cord-271701-tx0lqgff	231	25	and	and	CC
cord-271701-tx0lqgff	231	26	R	r	NN
cord-271701-tx0lqgff	231	27	for	for	IN
cord-271701-tx0lqgff	231	28	RTP	RTP	NNP
cord-271701-tx0lqgff	231	29	)	)	-RRB-
cord-271701-tx0lqgff	231	30	.	.	.
cord-271701-tx0lqgff	232	1	precedent	precedent	NN
cord-271701-tx0lqgff	232	2	,	,	,
cord-271701-tx0lqgff	232	3	since	since	IN
cord-271701-tx0lqgff	232	4	also	also	RB
cord-271701-tx0lqgff	232	5	the	the	DT
cord-271701-tx0lqgff	232	6	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	232	7	3Dpol	3Dpol	NNP
cord-271701-tx0lqgff	232	8	is	be	VBZ
cord-271701-tx0lqgff	232	9	inactive	inactive	JJ
cord-271701-tx0lqgff	232	10	as	as	RB
cord-271701-tx0lqgff	232	11	long	long	RB
cord-271701-tx0lqgff	232	12	as	as	IN
cord-271701-tx0lqgff	232	13	it	-PRON-	PRP
cord-271701-tx0lqgff	232	14	is	be	VBZ
cord-271701-tx0lqgff	232	15	fused	fuse	VBN
cord-271701-tx0lqgff	232	16	to	to	IN
cord-271701-tx0lqgff	232	17	the	the	DT
cord-271701-tx0lqgff	232	18	3C	3C	NNP
cord-271701-tx0lqgff	232	19	protease	protease	NN
cord-271701-tx0lqgff	232	20	in	in	IN
cord-271701-tx0lqgff	232	21	the	the	DT
cord-271701-tx0lqgff	232	22	3CD	3cd	CD
cord-271701-tx0lqgff	232	23	precursor	precursor	NN
cord-271701-tx0lqgff	232	24	(	(	-LRB-
cord-271701-tx0lqgff	232	25	29	29	CD
cord-271701-tx0lqgff	232	26	)	)	-RRB-
cord-271701-tx0lqgff	232	27	.	.	.
cord-271701-tx0lqgff	233	1	To	to	TO
cord-271701-tx0lqgff	233	2	verify	verify	VB
cord-271701-tx0lqgff	233	3	this	this	DT
cord-271701-tx0lqgff	233	4	hypothesis	hypothesis	NN
cord-271701-tx0lqgff	233	5	,	,	,
cord-271701-tx0lqgff	233	6	we	-PRON-	PRP
cord-271701-tx0lqgff	233	7	expressed	express	VBD
cord-271701-tx0lqgff	233	8	nsp7	nsp7	JJ
cord-271701-tx0lqgff	233	9	-	-	HYPH
cord-271701-tx0lqgff	233	10	8-His	8-His	NNP
cord-271701-tx0lqgff	233	11	and	and	CC
cord-271701-tx0lqgff	233	12	tested	test	VBD
cord-271701-tx0lqgff	233	13	this	this	DT
cord-271701-tx0lqgff	233	14	protein	protein	NN
cord-271701-tx0lqgff	233	15	for	for	IN
cord-271701-tx0lqgff	233	16	RdRp	RdRp	NNP
cord-271701-tx0lqgff	233	17	activity	activity	NN
cord-271701-tx0lqgff	233	18	.	.	.
cord-271701-tx0lqgff	234	1	Interestingly	interestingly	RB
cord-271701-tx0lqgff	234	2	,	,	,
cord-271701-tx0lqgff	234	3	this	this	DT
cord-271701-tx0lqgff	234	4	fusion	fusion	NN
cord-271701-tx0lqgff	234	5	protein	protein	NN
cord-271701-tx0lqgff	234	6	,	,	,
cord-271701-tx0lqgff	234	7	a	a	DT
cord-271701-tx0lqgff	234	8	potential	potential	JJ
cord-271701-tx0lqgff	234	9	intermediate	intermediate	NN
cord-271701-tx0lqgff	234	10	of	of	IN
cord-271701-tx0lqgff	234	11	CoV	CoV	NNP
cord-271701-tx0lqgff	234	12	replicase	replicase	NNP
cord-271701-tx0lqgff	234	13	polyprotein	polyprotein	NNP
cord-271701-tx0lqgff	234	14	processing	processing	NNP
cord-271701-tx0lqgff	234	15	and	and	CC
cord-271701-tx0lqgff	234	16	a	a	DT
cord-271701-tx0lqgff	234	17	multimer	multimer	NN
cord-271701-tx0lqgff	234	18	in	in	IN
cord-271701-tx0lqgff	234	19	solution	solution	NN
cord-271701-tx0lqgff	234	20	(	(	-LRB-
cord-271701-tx0lqgff	234	21	Figure	Figure	NNP
cord-271701-tx0lqgff	234	22	7E	7E	NNP
cord-271701-tx0lqgff	234	23	)	)	-RRB-
cord-271701-tx0lqgff	234	24	,	,	,
cord-271701-tx0lqgff	234	25	showed	show	VBD
cord-271701-tx0lqgff	234	26	primer	primer	NN
cord-271701-tx0lqgff	234	27	extension	extension	NN
cord-271701-tx0lqgff	234	28	activities	activity	NNS
cord-271701-tx0lqgff	234	29	that	that	WDT
cord-271701-tx0lqgff	234	30	were	be	VBD
cord-271701-tx0lqgff	234	31	comparable	comparable	JJ
cord-271701-tx0lqgff	234	32	to	to	IN
cord-271701-tx0lqgff	234	33	or	or	CC
cord-271701-tx0lqgff	234	34	higher	high	JJR
cord-271701-tx0lqgff	234	35	than	than	IN
cord-271701-tx0lqgff	234	36	the	the	DT
cord-271701-tx0lqgff	234	37	activity	activity	NN
cord-271701-tx0lqgff	234	38	of	of	IN
cord-271701-tx0lqgff	234	39	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	234	40	+	+	SYM
cord-271701-tx0lqgff	234	41	8-His	8-his	NN
cord-271701-tx0lqgff	234	42	)	)	-RRB-
cord-271701-tx0lqgff	235	1	(	(	-LRB-
cord-271701-tx0lqgff	235	2	Figure	figure	NN
cord-271701-tx0lqgff	235	3	7F	7f	CD
cord-271701-tx0lqgff	235	4	)	)	-RRB-
cord-271701-tx0lqgff	235	5	.	.	.
cord-271701-tx0lqgff	236	1	The	the	DT
cord-271701-tx0lqgff	236	2	de	de	FW
cord-271701-tx0lqgff	236	3	novo	novo	NNP
cord-271701-tx0lqgff	236	4	initiation	initiation	NN
cord-271701-tx0lqgff	236	5	activity	activity	NN
cord-271701-tx0lqgff	236	6	of	of	IN
cord-271701-tx0lqgff	236	7	nsp7	nsp7	NNP
cord-271701-tx0lqgff	236	8	-	-	HYPH
cord-271701-tx0lqgff	236	9	8-His	8-His	NNP
cord-271701-tx0lqgff	236	10	was	be	VBD
cord-271701-tx0lqgff	236	11	,	,	,
cord-271701-tx0lqgff	236	12	however	however	RB
cord-271701-tx0lqgff	236	13	,	,	,
cord-271701-tx0lqgff	236	14	$	$	$
cord-271701-tx0lqgff	236	15	2-fold	2-fold	CD
cord-271701-tx0lqgff	236	16	lower	low	JJR
cord-271701-tx0lqgff	236	17	than	than	IN
cord-271701-tx0lqgff	236	18	the	the	DT
cord-271701-tx0lqgff	236	19	activity	activity	NN
cord-271701-tx0lqgff	236	20	of	of	IN
cord-271701-tx0lqgff	236	21	nsp8	nsp8	NN
cord-271701-tx0lqgff	236	22	and	and	CC
cord-271701-tx0lqgff	236	23	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	236	24	+	+	SYM
cord-271701-tx0lqgff	236	25	8	8	CD
cord-271701-tx0lqgff	236	26	)	)	-RRB-
cord-271701-tx0lqgff	237	1	(	(	-LRB-
cord-271701-tx0lqgff	237	2	Figure	Figure	NNP
cord-271701-tx0lqgff	237	3	7D	7D	NNP
cord-271701-tx0lqgff	237	4	)	)	-RRB-
cord-271701-tx0lqgff	237	5	.	.	.
cord-271701-tx0lqgff	238	1	In	in	IN
cord-271701-tx0lqgff	238	2	conclusion	conclusion	NN
cord-271701-tx0lqgff	238	3	,	,	,
cord-271701-tx0lqgff	238	4	this	this	DT
cord-271701-tx0lqgff	238	5	result	result	NN
cord-271701-tx0lqgff	238	6	clearly	clearly	RB
cord-271701-tx0lqgff	238	7	underlines	underline	VBZ
cord-271701-tx0lqgff	238	8	that	that	IN
cord-271701-tx0lqgff	238	9	the	the	DT
cord-271701-tx0lqgff	238	10	two	two	CD
cord-271701-tx0lqgff	238	11	N	n	CD
cord-271701-tx0lqgff	238	12	-	-	HYPH
cord-271701-tx0lqgff	238	13	terminal	terminal	NN
cord-271701-tx0lqgff	238	14	fusion	fusion	NN
cord-271701-tx0lqgff	238	15	partners	partner	NNS
cord-271701-tx0lqgff	238	16	other	other	JJ
cord-271701-tx0lqgff	238	17	than	than	IN
cord-271701-tx0lqgff	238	18	nsp7	nsp7	PRP
cord-271701-tx0lqgff	238	19	are	be	VBP
cord-271701-tx0lqgff	238	20	specifically	specifically	RB
cord-271701-tx0lqgff	238	21	detrimental	detrimental	JJ
cord-271701-tx0lqgff	238	22	to	to	IN
cord-271701-tx0lqgff	238	23	SARS	SARS	NNP
cord-271701-tx0lqgff	238	24	-	-	HYPH
cord-271701-tx0lqgff	238	25	CoV	CoV	NNP
cord-271701-tx0lqgff	238	26	nsp8	nsp8	NNP
cord-271701-tx0lqgff	238	27	primer	primer	NN
cord-271701-tx0lqgff	238	28	-	-	HYPH
cord-271701-tx0lqgff	238	29	dependent	dependent	JJ
cord-271701-tx0lqgff	238	30	RdRp	RdRp	NNP
cord-271701-tx0lqgff	238	31	activity	activity	NN
cord-271701-tx0lqgff	238	32	in	in	IN
cord-271701-tx0lqgff	238	33	vitro	vitro	FW
cord-271701-tx0lqgff	238	34	.	.	.
cord-271701-tx0lqgff	239	1	It	-PRON-	PRP
cord-271701-tx0lqgff	239	2	also	also	RB
cord-271701-tx0lqgff	239	3	demonstrates	demonstrate	VBZ
cord-271701-tx0lqgff	239	4	that	that	DT
cord-271701-tx0lqgff	239	5	nsp8	nsp8	NN
cord-271701-tx0lqgff	239	6	alone	alone	RB
cord-271701-tx0lqgff	239	7	may	may	MD
cord-271701-tx0lqgff	239	8	be	be	VB
cord-271701-tx0lqgff	239	9	sufficient	sufficient	JJ
cord-271701-tx0lqgff	239	10	to	to	TO
cord-271701-tx0lqgff	239	11	act	act	VB
cord-271701-tx0lqgff	239	12	as	as	IN
cord-271701-tx0lqgff	239	13	a	a	DT
cord-271701-tx0lqgff	239	14	primase	primase	NN
cord-271701-tx0lqgff	239	15	.	.	.
cord-271701-tx0lqgff	240	1	The	the	DT
cord-271701-tx0lqgff	240	2	complex	complex	JJ
cord-271701-tx0lqgff	240	3	replication	replication	NN
cord-271701-tx0lqgff	240	4	and	and	CC
cord-271701-tx0lqgff	240	5	transcription	transcription	NN
cord-271701-tx0lqgff	240	6	process	process	NN
cord-271701-tx0lqgff	240	7	that	that	WDT
cord-271701-tx0lqgff	240	8	coronaviruses	coronaviruse	NNS
cord-271701-tx0lqgff	240	9	initiate	initiate	VBP
cord-271701-tx0lqgff	240	10	upon	upon	IN
cord-271701-tx0lqgff	240	11	infection	infection	NN
cord-271701-tx0lqgff	240	12	involves	involve	VBZ
cord-271701-tx0lqgff	240	13	up	up	IN
cord-271701-tx0lqgff	240	14	to	to	TO
cord-271701-tx0lqgff	240	15	16	16	CD
cord-271701-tx0lqgff	240	16	viral	viral	JJ
cord-271701-tx0lqgff	240	17	nsps	nsp	NNS
cord-271701-tx0lqgff	240	18	and	and	CC
cord-271701-tx0lqgff	240	19	at	at	IN
cord-271701-tx0lqgff	240	20	least	least	RBS
cord-271701-tx0lqgff	240	21	one	one	CD
cord-271701-tx0lqgff	240	22	host	host	NN
cord-271701-tx0lqgff	240	23	factor	factor	NN
cord-271701-tx0lqgff	240	24	(	(	-LRB-
cord-271701-tx0lqgff	240	25	30	30	CD
cord-271701-tx0lqgff	240	26	)	)	-RRB-
cord-271701-tx0lqgff	240	27	(	(	-LRB-
cord-271701-tx0lqgff	240	28	31	31	CD
cord-271701-tx0lqgff	240	29	)	)	-RRB-
cord-271701-tx0lqgff	240	30	(	(	-LRB-
cord-271701-tx0lqgff	240	31	32	32	CD
cord-271701-tx0lqgff	240	32	)	)	-RRB-
cord-271701-tx0lqgff	240	33	.	.	.
cord-271701-tx0lqgff	241	1	Both	both	DT
cord-271701-tx0lqgff	241	2	individually	individually	RB
cord-271701-tx0lqgff	241	3	as	as	RB
cord-271701-tx0lqgff	241	4	well	well	RB
cord-271701-tx0lqgff	241	5	as	as	IN
cord-271701-tx0lqgff	241	6	in	in	IN
cord-271701-tx0lqgff	241	7	complex	complex	NN
cord-271701-tx0lqgff	241	8	with	with	IN
cord-271701-tx0lqgff	241	9	each	each	DT
cord-271701-tx0lqgff	241	10	other	other	JJ
cord-271701-tx0lqgff	241	11	,	,	,
cord-271701-tx0lqgff	241	12	these	these	DT
cord-271701-tx0lqgff	241	13	subunits	subunit	NNS
cord-271701-tx0lqgff	241	14	engage	engage	VBP
cord-271701-tx0lqgff	241	15	in	in	IN
cord-271701-tx0lqgff	241	16	numerous	numerous	JJ
cord-271701-tx0lqgff	241	17	protein	protein	NN
cord-271701-tx0lqgff	241	18	-	-	HYPH
cord-271701-tx0lqgff	241	19	protein	protein	NN
cord-271701-tx0lqgff	241	20	interactions	interaction	NNS
cord-271701-tx0lqgff	241	21	(	(	-LRB-
cord-271701-tx0lqgff	241	22	17	17	CD
cord-271701-tx0lqgff	241	23	,	,	,
cord-271701-tx0lqgff	241	24	33	33	CD
cord-271701-tx0lqgff	241	25	)	)	-RRB-
cord-271701-tx0lqgff	241	26	and	and	CC
cord-271701-tx0lqgff	241	27	embody	embody	VB
cord-271701-tx0lqgff	241	28	various	various	JJ
cord-271701-tx0lqgff	241	29	enzymatic	enzymatic	JJ
cord-271701-tx0lqgff	241	30	activities	activity	NNS
cord-271701-tx0lqgff	241	31	,	,	,
cord-271701-tx0lqgff	241	32	including	include	VBG
cord-271701-tx0lqgff	241	33	proteolytic	proteolytic	JJ
cord-271701-tx0lqgff	241	34	(	(	-LRB-
cord-271701-tx0lqgff	241	35	19	19	CD
cord-271701-tx0lqgff	241	36	,	,	,
cord-271701-tx0lqgff	241	37	34	34	CD
cord-271701-tx0lqgff	241	38	)	)	-RRB-
cord-271701-tx0lqgff	241	39	,	,	,
cord-271701-tx0lqgff	241	40	ATPase	ATPase	NNP
cord-271701-tx0lqgff	241	41	(	(	-LRB-
cord-271701-tx0lqgff	241	42	35	35	CD
cord-271701-tx0lqgff	241	43	)	)	-RRB-
cord-271701-tx0lqgff	241	44	,	,	,
cord-271701-tx0lqgff	241	45	and	and	CC
cord-271701-tx0lqgff	241	46	5	5	CD
cord-271701-tx0lqgff	241	47	0	0	CD
cord-271701-tx0lqgff	241	48	cap	cap	NN
cord-271701-tx0lqgff	241	49	modifying	modify	VBG
cord-271701-tx0lqgff	241	50	reactions	reaction	NNS
cord-271701-tx0lqgff	241	51	(	(	-LRB-
cord-271701-tx0lqgff	241	52	36	36	CD
cord-271701-tx0lqgff	241	53	)	)	-RRB-
cord-271701-tx0lqgff	241	54	.	.	.
cord-271701-tx0lqgff	242	1	Remarkably	remarkably	RB
cord-271701-tx0lqgff	242	2	though	though	RB
cord-271701-tx0lqgff	242	3	,	,	,
cord-271701-tx0lqgff	242	4	the	the	DT
cord-271701-tx0lqgff	242	5	mechanism	mechanism	NN
cord-271701-tx0lqgff	242	6	and	and	CC
cord-271701-tx0lqgff	242	7	enzymes	enzyme	NNS
cord-271701-tx0lqgff	242	8	required	require	VBN
cord-271701-tx0lqgff	242	9	to	to	TO
cord-271701-tx0lqgff	242	10	catalyse	catalyse	VB
cord-271701-tx0lqgff	242	11	RNA	RNA	NNP
cord-271701-tx0lqgff	242	12	synthesis	synthesis	NN
cord-271701-tx0lqgff	242	13	in	in	IN
cord-271701-tx0lqgff	242	14	the	the	DT
cord-271701-tx0lqgff	242	15	CoV	cov	NN
cord-271701-tx0lqgff	242	16	RTC	RTC	NNP
cord-271701-tx0lqgff	242	17	remain	remain	VBP
cord-271701-tx0lqgff	242	18	very	very	RB
cord-271701-tx0lqgff	242	19	poorly	poorly	RB
cord-271701-tx0lqgff	242	20	understood	understand	VBN
cord-271701-tx0lqgff	242	21	.	.	.
cord-271701-tx0lqgff	243	1	Moreover	moreover	RB
cord-271701-tx0lqgff	243	2	,	,	,
cord-271701-tx0lqgff	243	3	uniquely	uniquely	RB
cord-271701-tx0lqgff	243	4	among	among	IN
cord-271701-tx0lqgff	243	5	RNA	RNA	NNP
cord-271701-tx0lqgff	243	6	viruses	virus	NNS
cord-271701-tx0lqgff	243	7	which	which	WDT
cord-271701-tx0lqgff	243	8	generally	generally	RB
cord-271701-tx0lqgff	243	9	employ	employ	VBP
cord-271701-tx0lqgff	243	10	a	a	DT
cord-271701-tx0lqgff	243	11	single	single	JJ
cord-271701-tx0lqgff	243	12	RNA	RNA	NNP
cord-271701-tx0lqgff	243	13	polymerase	polymerase	NN
cord-271701-tx0lqgff	243	14	to	to	TO
cord-271701-tx0lqgff	243	15	drive	drive	VB
cord-271701-tx0lqgff	243	16	their	-PRON-	PRP$
cord-271701-tx0lqgff	243	17	RNA	RNA	NNP
cord-271701-tx0lqgff	243	18	synthesis	synthesis	NN
cord-271701-tx0lqgff	243	19	(	(	-LRB-
cord-271701-tx0lqgff	243	20	1	1	CD
cord-271701-tx0lqgff	243	21	,	,	,
cord-271701-tx0lqgff	243	22	2	2	CD
cord-271701-tx0lqgff	243	23	)	)	-RRB-
cord-271701-tx0lqgff	243	24	,	,	,
cord-271701-tx0lqgff	243	25	the	the	DT
cord-271701-tx0lqgff	243	26	polymerase	polymerase	NN
cord-271701-tx0lqgff	243	27	activity	activity	NN
cord-271701-tx0lqgff	243	28	assays	assay	NNS
cord-271701-tx0lqgff	243	29	and	and	CC
cord-271701-tx0lqgff	243	30	nsp8	nsp8	NN
cord-271701-tx0lqgff	243	31	mutagenesis	mutagenesis	NN
cord-271701-tx0lqgff	243	32	documented	document	VBN
cord-271701-tx0lqgff	243	33	in	in	IN
cord-271701-tx0lqgff	243	34	this	this	DT
cord-271701-tx0lqgff	243	35	and	and	CC
cord-271701-tx0lqgff	243	36	other	other	JJ
cord-271701-tx0lqgff	243	37	studies	study	NNS
cord-271701-tx0lqgff	243	38	suggest	suggest	VBP
cord-271701-tx0lqgff	243	39	that	that	IN
cord-271701-tx0lqgff	243	40	,	,	,
cord-271701-tx0lqgff	243	41	in	in	IN
cord-271701-tx0lqgff	243	42	addition	addition	NN
cord-271701-tx0lqgff	243	43	to	to	IN
cord-271701-tx0lqgff	243	44	the	the	DT
cord-271701-tx0lqgff	243	45	presumed	presume	VBN
cord-271701-tx0lqgff	243	46	nsp12	nsp12	NNP
cord-271701-tx0lqgff	243	47	'	'	POS
cord-271701-tx0lqgff	243	48	main	main	JJ
cord-271701-tx0lqgff	243	49	RdRp	RdRp	NNP
cord-271701-tx0lqgff	243	50	'	'	''
cord-271701-tx0lqgff	243	51	,	,	,
cord-271701-tx0lqgff	243	52	other	other	JJ
cord-271701-tx0lqgff	243	53	polymerase	polymerase	NN
cord-271701-tx0lqgff	243	54	activities	activity	NNS
cord-271701-tx0lqgff	243	55	could	could	MD
cord-271701-tx0lqgff	243	56	play	play	VB
cord-271701-tx0lqgff	243	57	a	a	DT
cord-271701-tx0lqgff	243	58	critical	critical	JJ
cord-271701-tx0lqgff	243	59	role	role	NN
cord-271701-tx0lqgff	243	60	in	in	IN
cord-271701-tx0lqgff	243	61	the	the	DT
cord-271701-tx0lqgff	243	62	synthesis	synthesis	NN
cord-271701-tx0lqgff	243	63	of	of	IN
cord-271701-tx0lqgff	243	64	SARS	SARS	NNP
cord-271701-tx0lqgff	243	65	-	-	HYPH
cord-271701-tx0lqgff	243	66	CoV	CoV	NNP
cord-271701-tx0lqgff	243	67	RNAs	rna	NNS
cord-271701-tx0lqgff	243	68	(	(	-LRB-
cord-271701-tx0lqgff	243	69	10	10	CD
cord-271701-tx0lqgff	243	70	,	,	,
cord-271701-tx0lqgff	243	71	12	12	CD
cord-271701-tx0lqgff	243	72	,	,	,
cord-271701-tx0lqgff	243	73	37	37	CD
cord-271701-tx0lqgff	243	74	,	,	,
cord-271701-tx0lqgff	243	75	38	38	CD
cord-271701-tx0lqgff	243	76	)	)	-RRB-
cord-271701-tx0lqgff	243	77	.	.	.
cord-271701-tx0lqgff	244	1	Following	follow	VBG
cord-271701-tx0lqgff	244	2	up	up	RP
cord-271701-tx0lqgff	244	3	on	on	IN
cord-271701-tx0lqgff	244	4	the	the	DT
cord-271701-tx0lqgff	244	5	description	description	NN
cord-271701-tx0lqgff	244	6	of	of	IN
cord-271701-tx0lqgff	244	7	an	an	DT
cord-271701-tx0lqgff	244	8	nsp8-and	nsp8-and	DT
cord-271701-tx0lqgff	244	9	nsp7-containing	nsp7-containing	NNP
cord-271701-tx0lqgff	244	10	hexadecameric	hexadecameric	NNP
cord-271701-tx0lqgff	244	11	ring	ring	NN
cord-271701-tx0lqgff	244	12	structure	structure	NN
cord-271701-tx0lqgff	244	13	(	(	-LRB-
cord-271701-tx0lqgff	244	14	13	13	CD
cord-271701-tx0lqgff	244	15	)	)	-RRB-
cord-271701-tx0lqgff	244	16	and	and	CC
cord-271701-tx0lqgff	244	17	the	the	DT
cord-271701-tx0lqgff	244	18	nsp8-associated	nsp8-associated	NNP
cord-271701-tx0lqgff	244	19	polymerase	polymerase	NN
cord-271701-tx0lqgff	244	20	activity	activity	NN
cord-271701-tx0lqgff	244	21	(	(	-LRB-
cord-271701-tx0lqgff	244	22	12	12	CD
cord-271701-tx0lqgff	244	23	)	)	-RRB-
cord-271701-tx0lqgff	244	24	,	,	,
cord-271701-tx0lqgff	244	25	we	-PRON-	PRP
cord-271701-tx0lqgff	244	26	here	here	RB
cord-271701-tx0lqgff	244	27	demonstrate	demonstrate	VBP
cord-271701-tx0lqgff	244	28	that	that	IN
cord-271701-tx0lqgff	244	29	the	the	DT
cord-271701-tx0lqgff	244	30	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	244	31	+	+	SYM
cord-271701-tx0lqgff	244	32	8	8	CD
cord-271701-tx0lqgff	244	33	)	)	-RRB-
cord-271701-tx0lqgff	244	34	hexadecamer	hexadecamer	NNP
cord-271701-tx0lqgff	244	35	is	be	VBZ
cord-271701-tx0lqgff	244	36	the	the	DT
cord-271701-tx0lqgff	244	37	most	most	RBS
cord-271701-tx0lqgff	244	38	probable	probable	JJ
cord-271701-tx0lqgff	244	39	conformation	conformation	NN
cord-271701-tx0lqgff	244	40	of	of	IN
cord-271701-tx0lqgff	244	41	the	the	DT
cord-271701-tx0lqgff	244	42	second	second	JJ
cord-271701-tx0lqgff	244	43	SARS	SARS	NNP
cord-271701-tx0lqgff	244	44	-	-	HYPH
cord-271701-tx0lqgff	244	45	CoV	CoV	NNP
cord-271701-tx0lqgff	244	46	polymerase	polymerase	NN
cord-271701-tx0lqgff	244	47	,	,	,
cord-271701-tx0lqgff	244	48	given	give	VBN
cord-271701-tx0lqgff	244	49	the	the	DT
cord-271701-tx0lqgff	244	50	near	near	RB
cord-271701-tx0lqgff	244	51	-	-	HYPH
cord-271701-tx0lqgff	244	52	complete	complete	NN
cord-271701-tx0lqgff	244	53	association	association	NN
cord-271701-tx0lqgff	244	54	of	of	IN
cord-271701-tx0lqgff	244	55	nsp7	nsp7	NNP
cord-271701-tx0lqgff	244	56	and	and	CC
cord-271701-tx0lqgff	244	57	nsp8	nsp8	NN
cord-271701-tx0lqgff	244	58	when	when	WRB
cord-271701-tx0lqgff	244	59	mixed	mix	VBN
cord-271701-tx0lqgff	244	60	1:1	1:1	CD
cord-271701-tx0lqgff	244	61	in	in	IN
cord-271701-tx0lqgff	244	62	solution	solution	NN
cord-271701-tx0lqgff	244	63	(	(	-LRB-
cord-271701-tx0lqgff	244	64	Figure	figure	NN
cord-271701-tx0lqgff	244	65	2F	2f	NN
cord-271701-tx0lqgff	244	66	)	)	-RRB-
cord-271701-tx0lqgff	244	67	.	.	.
cord-271701-tx0lqgff	245	1	Significant	significant	JJ
cord-271701-tx0lqgff	245	2	for	for	IN
cord-271701-tx0lqgff	245	3	our	-PRON-	PRP$
cord-271701-tx0lqgff	245	4	understanding	understanding	NN
cord-271701-tx0lqgff	245	5	of	of	IN
cord-271701-tx0lqgff	245	6	CoV	CoV	NNP
cord-271701-tx0lqgff	245	7	RNA	RNA	NNP
cord-271701-tx0lqgff	245	8	synthesis	synthesis	NN
cord-271701-tx0lqgff	245	9	,	,	,
cord-271701-tx0lqgff	245	10	we	-PRON-	PRP
cord-271701-tx0lqgff	245	11	find	find	VBP
cord-271701-tx0lqgff	245	12	that	that	IN
cord-271701-tx0lqgff	245	13	this	this	DT
cord-271701-tx0lqgff	245	14	complex	complex	NN
cord-271701-tx0lqgff	245	15	is	be	VBZ
cord-271701-tx0lqgff	245	16	capable	capable	JJ
cord-271701-tx0lqgff	245	17	of	of	IN
cord-271701-tx0lqgff	245	18	binding	bind	VBG
cord-271701-tx0lqgff	245	19	dsRNA	dsrna	NN
cord-271701-tx0lqgff	245	20	molecules	molecule	NNS
cord-271701-tx0lqgff	245	21	and	and	CC
cord-271701-tx0lqgff	245	22	extending	extend	VBG
cord-271701-tx0lqgff	245	23	partially	partially	RB
cord-271701-tx0lqgff	245	24	double	double	RB
cord-271701-tx0lqgff	245	25	-	-	HYPH
cord-271701-tx0lqgff	245	26	stranded	strand	VBN
cord-271701-tx0lqgff	245	27	RNA	rna	NN
cord-271701-tx0lqgff	245	28	templates	template	NNS
cord-271701-tx0lqgff	245	29	.	.	.
cord-271701-tx0lqgff	246	1	This	this	DT
cord-271701-tx0lqgff	246	2	activity	activity	NN
cord-271701-tx0lqgff	246	3	is	be	VBZ
cord-271701-tx0lqgff	246	4	therefore	therefore	RB
cord-271701-tx0lqgff	246	5	essentially	essentially	RB
cord-271701-tx0lqgff	246	6	comparable	comparable	JJ
cord-271701-tx0lqgff	246	7	to	to	IN
cord-271701-tx0lqgff	246	8	the	the	DT
cord-271701-tx0lqgff	246	9	activity	activity	NN
cord-271701-tx0lqgff	246	10	reported	report	VBN
cord-271701-tx0lqgff	246	11	for	for	IN
cord-271701-tx0lqgff	246	12	the	the	DT
cord-271701-tx0lqgff	246	13	nsp12-RdRp	nsp12-rdrp	NN
cord-271701-tx0lqgff	246	14	(	(	-LRB-
cord-271701-tx0lqgff	246	15	10	10	CD
cord-271701-tx0lqgff	246	16	)	)	-RRB-
cord-271701-tx0lqgff	246	17	.	.	.
cord-271701-tx0lqgff	247	1	A	a	DT
cord-271701-tx0lqgff	247	2	direct	direct	JJ
cord-271701-tx0lqgff	247	3	comparison	comparison	NN
cord-271701-tx0lqgff	247	4	with	with	IN
cord-271701-tx0lqgff	247	5	the	the	DT
cord-271701-tx0lqgff	247	6	nsp12	nsp12	CD
cord-271701-tx0lqgff	247	7	activity	activity	NN
cord-271701-tx0lqgff	247	8	is	be	VBZ
cord-271701-tx0lqgff	247	9	difficult	difficult	JJ
cord-271701-tx0lqgff	247	10	,	,	,
cord-271701-tx0lqgff	247	11	however	however	RB
cord-271701-tx0lqgff	247	12	.	.	.
cord-271701-tx0lqgff	248	1	In	in	IN
cord-271701-tx0lqgff	248	2	the	the	DT
cord-271701-tx0lqgff	248	3	course	course	NN
cord-271701-tx0lqgff	248	4	of	of	IN
cord-271701-tx0lqgff	248	5	a	a	DT
cord-271701-tx0lqgff	248	6	one	one	CD
cord-271701-tx0lqgff	248	7	-	-	HYPH
cord-271701-tx0lqgff	248	8	hour	hour	NN
cord-271701-tx0lqgff	248	9	reaction	reaction	NN
cord-271701-tx0lqgff	248	10	,	,	,
cord-271701-tx0lqgff	248	11	0.1	0.1	CD
cord-271701-tx0lqgff	248	12	mM	mm	CD
cord-271701-tx0lqgff	248	13	monomeric	monomeric	JJ
cord-271701-tx0lqgff	248	14	nsp12-RdRp	nsp12-RdRp	NNP
cord-271701-tx0lqgff	248	15	incorporates	incorporate	VBZ
cord-271701-tx0lqgff	248	16	$	$	$
cord-271701-tx0lqgff	248	17	2	2	CD
cord-271701-tx0lqgff	248	18	mM	mm	CD
cord-271701-tx0lqgff	248	19	NMP	NMP	NNP
cord-271701-tx0lqgff	248	20	into	into	IN
cord-271701-tx0lqgff	248	21	a	a	DT
cord-271701-tx0lqgff	248	22	primed	primed	JJ
cord-271701-tx0lqgff	248	23	(	(	-LRB-
cord-271701-tx0lqgff	248	24	CU	cu	NN
cord-271701-tx0lqgff	248	25	)	)	-RRB-
cord-271701-tx0lqgff	248	26	10	10	CD
cord-271701-tx0lqgff	248	27	template	template	NN
cord-271701-tx0lqgff	248	28	(	(	-LRB-
cord-271701-tx0lqgff	248	29	10	10	CD
cord-271701-tx0lqgff	248	30	)	)	-RRB-
cord-271701-tx0lqgff	248	31	.	.	.
cord-271701-tx0lqgff	249	1	The	the	DT
cord-271701-tx0lqgff	249	2	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	249	3	+	+	SYM
cord-271701-tx0lqgff	249	4	8	8	CD
cord-271701-tx0lqgff	249	5	)	)	-RRB-
cord-271701-tx0lqgff	249	6	complex	complex	NN
cord-271701-tx0lqgff	249	7	,	,	,
cord-271701-tx0lqgff	249	8	at	at	IN
cord-271701-tx0lqgff	249	9	a	a	DT
cord-271701-tx0lqgff	249	10	1	1	CD
cord-271701-tx0lqgff	249	11	mM	mm	CD
cord-271701-tx0lqgff	249	12	concentration	concentration	NN
cord-271701-tx0lqgff	249	13	of	of	IN
cord-271701-tx0lqgff	249	14	nsp7	nsp7	NN
cord-271701-tx0lqgff	249	15	and	and	CC
cord-271701-tx0lqgff	249	16	nsp8	nsp8	JJ
cord-271701-tx0lqgff	249	17	monomers	monomer	NNS
cord-271701-tx0lqgff	249	18	,	,	,
cord-271701-tx0lqgff	249	19	incorporates	incorporate	VBZ
cord-271701-tx0lqgff	249	20	$	$	$
cord-271701-tx0lqgff	249	21	1	1	CD
cord-271701-tx0lqgff	249	22	mM	mm	CD
cord-271701-tx0lqgff	249	23	NMP	NMP	NNP
cord-271701-tx0lqgff	249	24	.	.	.
cord-271701-tx0lqgff	250	1	Per	per	IN
cord-271701-tx0lqgff	250	2	monomer	monomer	NN
cord-271701-tx0lqgff	250	3	,	,	,
cord-271701-tx0lqgff	250	4	the	the	DT
cord-271701-tx0lqgff	250	5	activity	activity	NN
cord-271701-tx0lqgff	250	6	difference	difference	NN
cord-271701-tx0lqgff	250	7	is	be	VBZ
cord-271701-tx0lqgff	250	8	therefore	therefore	RB
cord-271701-tx0lqgff	250	9	20-fold	20-fold	CD
cord-271701-tx0lqgff	250	10	,	,	,
cord-271701-tx0lqgff	250	11	but	but	CC
cord-271701-tx0lqgff	250	12	if	if	IN
cord-271701-tx0lqgff	250	13	we	-PRON-	PRP
cord-271701-tx0lqgff	250	14	assume	assume	VBP
cord-271701-tx0lqgff	250	15	that	that	IN
cord-271701-tx0lqgff	250	16	most	most	RBS
cord-271701-tx0lqgff	250	17	nsp7	nsp7	JJ
cord-271701-tx0lqgff	250	18	and	and	CC
cord-271701-tx0lqgff	250	19	nsp8	nsp8	NN
cord-271701-tx0lqgff	250	20	monomers	monomer	NNS
cord-271701-tx0lqgff	250	21	assemble	assemble	VBP
cord-271701-tx0lqgff	250	22	into	into	IN
cord-271701-tx0lqgff	250	23	hexadecamers	hexadecamer	NNS
cord-271701-tx0lqgff	250	24	and	and	CC
cord-271701-tx0lqgff	250	25	that	that	IN
cord-271701-tx0lqgff	250	26	each	each	DT
cord-271701-tx0lqgff	250	27	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	250	28	contributes	contribute	VBZ
cord-271701-tx0lqgff	250	29	only	only	RB
cord-271701-tx0lqgff	250	30	one	one	CD
cord-271701-tx0lqgff	250	31	functional	functional	JJ
cord-271701-tx0lqgff	250	32	active	active	JJ
cord-271701-tx0lqgff	250	33	site	site	NN
cord-271701-tx0lqgff	250	34	per	per	IN
cord-271701-tx0lqgff	250	35	incorporation	incorporation	NN
cord-271701-tx0lqgff	250	36	event	event	NN
cord-271701-tx0lqgff	250	37	,	,	,
cord-271701-tx0lqgff	250	38	the	the	DT
cord-271701-tx0lqgff	250	39	difference	difference	NN
cord-271701-tx0lqgff	250	40	would	would	MD
cord-271701-tx0lqgff	250	41	be	be	VB
cord-271701-tx0lqgff	250	42	much	much	RB
cord-271701-tx0lqgff	250	43	smaller	small	JJR
cord-271701-tx0lqgff	250	44	and	and	CC
cord-271701-tx0lqgff	250	45	only	only	RB
cord-271701-tx0lqgff	250	46	$	$	$
cord-271701-tx0lqgff	250	47	2.5-fold	2.5-fold	CD
cord-271701-tx0lqgff	250	48	.	.	.
cord-271701-tx0lqgff	251	1	Presently	presently	RB
cord-271701-tx0lqgff	251	2	,	,	,
cord-271701-tx0lqgff	251	3	however	however	RB
cord-271701-tx0lqgff	251	4	,	,	,
cord-271701-tx0lqgff	251	5	we	-PRON-	PRP
cord-271701-tx0lqgff	251	6	do	do	VBP
cord-271701-tx0lqgff	251	7	not	not	RB
cord-271701-tx0lqgff	251	8	yet	yet	RB
cord-271701-tx0lqgff	251	9	have	have	VB
cord-271701-tx0lqgff	251	10	an	an	DT
cord-271701-tx0lqgff	251	11	estimate	estimate	NN
cord-271701-tx0lqgff	251	12	for	for	IN
cord-271701-tx0lqgff	251	13	the	the	DT
cord-271701-tx0lqgff	251	14	efficiency	efficiency	NN
cord-271701-tx0lqgff	251	15	and	and	CC
cord-271701-tx0lqgff	251	16	stability	stability	NN
cord-271701-tx0lqgff	251	17	of	of	IN
cord-271701-tx0lqgff	251	18	the	the	DT
cord-271701-tx0lqgff	251	19	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	251	20	+	+	SYM
cord-271701-tx0lqgff	251	21	8	8	CD
cord-271701-tx0lqgff	251	22	)	)	-RRB-
cord-271701-tx0lqgff	251	23	complex	complex	NN
cord-271701-tx0lqgff	251	24	,	,	,
cord-271701-tx0lqgff	251	25	nor	nor	CC
cord-271701-tx0lqgff	251	26	do	do	VBP
cord-271701-tx0lqgff	251	27	we	-PRON-	PRP
cord-271701-tx0lqgff	251	28	know	know	VB
cord-271701-tx0lqgff	251	29	the	the	DT
cord-271701-tx0lqgff	251	30	number	number	NN
cord-271701-tx0lqgff	251	31	of	of	IN
cord-271701-tx0lqgff	251	32	active	active	JJ
cord-271701-tx0lqgff	251	33	sites	site	NNS
cord-271701-tx0lqgff	251	34	in	in	IN
cord-271701-tx0lqgff	251	35	the	the	DT
cord-271701-tx0lqgff	251	36	complex	complex	NN
cord-271701-tx0lqgff	251	37	that	that	WDT
cord-271701-tx0lqgff	251	38	determine	determine	VBP
cord-271701-tx0lqgff	251	39	its	-PRON-	PRP$
cord-271701-tx0lqgff	251	40	overall	overall	JJ
cord-271701-tx0lqgff	251	41	activity	activity	NN
cord-271701-tx0lqgff	251	42	.	.	.
cord-271701-tx0lqgff	252	1	Mutagenesis	Mutagenesis	NNP
cord-271701-tx0lqgff	252	2	of	of	IN
cord-271701-tx0lqgff	252	3	nsp8	nsp8	NN
cord-271701-tx0lqgff	252	4	was	be	VBD
cord-271701-tx0lqgff	252	5	performed	perform	VBN
cord-271701-tx0lqgff	252	6	to	to	TO
cord-271701-tx0lqgff	252	7	identify	identify	VB
cord-271701-tx0lqgff	252	8	residues	residue	NNS
cord-271701-tx0lqgff	252	9	that	that	WDT
cord-271701-tx0lqgff	252	10	may	may	MD
cord-271701-tx0lqgff	252	11	contribute	contribute	VB
cord-271701-tx0lqgff	252	12	to	to	IN
cord-271701-tx0lqgff	252	13	the	the	DT
cord-271701-tx0lqgff	252	14	catalytic	catalytic	JJ
cord-271701-tx0lqgff	252	15	centre	centre	NN
cord-271701-tx0lqgff	252	16	of	of	IN
cord-271701-tx0lqgff	253	1	the	the	DT
cord-271701-tx0lqgff	253	2	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	253	3	+	+	SYM
cord-271701-tx0lqgff	253	4	8	8	CD
cord-271701-tx0lqgff	253	5	)	)	-RRB-
cord-271701-tx0lqgff	253	6	polymerase	polymerase	NN
cord-271701-tx0lqgff	253	7	,	,	,
cord-271701-tx0lqgff	253	8	while	while	IN
cord-271701-tx0lqgff	253	9	differently	differently	RB
cord-271701-tx0lqgff	253	10	tagged	tagged	NNP
cord-271701-tx0lqgff	253	11	nsp8	nsp8	NNP
cord-271701-tx0lqgff	253	12	recombinant	recombinant	JJ
cord-271701-tx0lqgff	253	13	proteins	protein	NNS
cord-271701-tx0lqgff	253	14	were	be	VBD
cord-271701-tx0lqgff	253	15	constructed	construct	VBN
cord-271701-tx0lqgff	253	16	to	to	TO
cord-271701-tx0lqgff	253	17	explain	explain	VB
cord-271701-tx0lqgff	253	18	some	some	DT
cord-271701-tx0lqgff	253	19	striking	striking	JJ
cord-271701-tx0lqgff	253	20	differences	difference	NNS
cord-271701-tx0lqgff	253	21	with	with	IN
cord-271701-tx0lqgff	253	22	previous	previous	JJ
cord-271701-tx0lqgff	253	23	observations	observation	NNS
cord-271701-tx0lqgff	253	24	.	.	.
cord-271701-tx0lqgff	254	1	These	these	DT
cord-271701-tx0lqgff	254	2	efforts	effort	NNS
cord-271701-tx0lqgff	254	3	resulted	result	VBD
cord-271701-tx0lqgff	254	4	in	in	IN
cord-271701-tx0lqgff	254	5	two	two	CD
cord-271701-tx0lqgff	254	6	intriguing	intriguing	JJ
cord-271701-tx0lqgff	254	7	observations	observation	NNS
cord-271701-tx0lqgff	254	8	.	.	.
cord-271701-tx0lqgff	255	1	Firstly	firstly	RB
cord-271701-tx0lqgff	255	2	,	,	,
cord-271701-tx0lqgff	255	3	mutation	mutation	NN
cord-271701-tx0lqgff	255	4	of	of	IN
cord-271701-tx0lqgff	255	5	the	the	DT
cord-271701-tx0lqgff	255	6	conserved	conserved	JJ
cord-271701-tx0lqgff	255	7	N	n	CD
cord-271701-tx0lqgff	255	8	-	-	HYPH
cord-271701-tx0lqgff	255	9	terminal	terminal	NN
cord-271701-tx0lqgff	255	10	D	D	NNP
cord-271701-tx0lqgff	255	11	/	/	SYM
cord-271701-tx0lqgff	255	12	ExD	ExD	NNP
cord-271701-tx0lqgff	255	13	/	/	SYM
cord-271701-tx0lqgff	255	14	E	e	NN
cord-271701-tx0lqgff	255	15	motif	motif	NN
cord-271701-tx0lqgff	255	16	,	,	,
cord-271701-tx0lqgff	255	17	comprising	comprise	VBG
cord-271701-tx0lqgff	255	18	D50	D50	NNP
cord-271701-tx0lqgff	255	19	Table	Table	NNP
cord-271701-tx0lqgff	255	20	1	1	CD
cord-271701-tx0lqgff	255	21	)	)	-RRB-
cord-271701-tx0lqgff	255	22	,	,	,
cord-271701-tx0lqgff	255	23	using	use	VBG
cord-271701-tx0lqgff	255	24	the	the	DT
cord-271701-tx0lqgff	255	25	synthesis	synthesis	NN
cord-271701-tx0lqgff	255	26	of	of	IN
cord-271701-tx0lqgff	255	27	the	the	DT
cord-271701-tx0lqgff	255	28	first	first	JJ
cord-271701-tx0lqgff	255	29	dinucleotide	dinucleotide	NN
cord-271701-tx0lqgff	255	30	pppGpA	pppGpA	NNP
cord-271701-tx0lqgff	255	31	,	,	,
cord-271701-tx0lqgff	255	32	as	as	IN
cord-271701-tx0lqgff	255	33	previously	previously	RB
cord-271701-tx0lqgff	255	34	described	describe	VBN
cord-271701-tx0lqgff	255	35	by	by	IN
cord-271701-tx0lqgff	255	36	Imbert	Imbert	NNP
cord-271701-tx0lqgff	255	37	et	et	NNP
cord-271701-tx0lqgff	255	38	al	al	NNP
cord-271701-tx0lqgff	255	39	.	.	.
cord-271701-tx0lqgff	256	1	(	(	-LRB-
cord-271701-tx0lqgff	256	2	12	12	CD
cord-271701-tx0lqgff	256	3	)	)	-RRB-
cord-271701-tx0lqgff	256	4	,	,	,
cord-271701-tx0lqgff	256	5	as	as	IN
cord-271701-tx0lqgff	256	6	readout	readout	NN
cord-271701-tx0lqgff	256	7	.	.	.
cord-271701-tx0lqgff	257	1	Nsp8	nsp8	JJ
cord-271701-tx0lqgff	257	2	template	template	NN
cord-271701-tx0lqgff	257	3	binding	bind	VBG
cord-271701-tx0lqgff	257	4	mutant	mutant	JJ
cord-271701-tx0lqgff	257	5	K58A	k58a	NN
cord-271701-tx0lqgff	257	6	was	be	VBD
cord-271701-tx0lqgff	257	7	used	use	VBN
cord-271701-tx0lqgff	257	8	as	as	IN
cord-271701-tx0lqgff	257	9	negative	negative	JJ
cord-271701-tx0lqgff	257	10	control	control	NN
cord-271701-tx0lqgff	257	11	.	.	.
cord-271701-tx0lqgff	258	1	The	the	DT
cord-271701-tx0lqgff	258	2	AMP	AMP	NNP
cord-271701-tx0lqgff	258	3	contaminant	contaminant	NN
cord-271701-tx0lqgff	258	4	present	present	NN
cord-271701-tx0lqgff	258	5	in	in	IN
cord-271701-tx0lqgff	258	6	the	the	DT
cord-271701-tx0lqgff	258	7	used	use	VBN
cord-271701-tx0lqgff	258	8	[	[	-LRB-
cord-271701-tx0lqgff	258	9	a-32	a-32	NNP
cord-271701-tx0lqgff	258	10	P]ATP	P]ATP	NNP
cord-271701-tx0lqgff	258	11	label	label	NN
cord-271701-tx0lqgff	258	12	is	be	VBZ
cord-271701-tx0lqgff	258	13	marked	mark	VBN
cord-271701-tx0lqgff	258	14	as	as	IN
cord-271701-tx0lqgff	258	15	loading	load	VBG
cord-271701-tx0lqgff	258	16	control	control	NN
cord-271701-tx0lqgff	258	17	and	and	CC
cord-271701-tx0lqgff	258	18	size	size	NN
cord-271701-tx0lqgff	258	19	reference	reference	NN
cord-271701-tx0lqgff	258	20	.	.	.
cord-271701-tx0lqgff	259	1	(	(	-LRB-
cord-271701-tx0lqgff	259	2	D	D	NNP
cord-271701-tx0lqgff	259	3	)	)	-RRB-
cord-271701-tx0lqgff	259	4	Side	side	NN
cord-271701-tx0lqgff	259	5	-	-	HYPH
cord-271701-tx0lqgff	259	6	by	by	IN
cord-271701-tx0lqgff	259	7	-	-	HYPH
cord-271701-tx0lqgff	259	8	side	side	NN
cord-271701-tx0lqgff	259	9	comparison	comparison	NN
cord-271701-tx0lqgff	259	10	of	of	IN
cord-271701-tx0lqgff	259	11	the	the	DT
cord-271701-tx0lqgff	259	12	de	de	FW
cord-271701-tx0lqgff	259	13	novo	novo	NNP
cord-271701-tx0lqgff	259	14	initiation	initiation	NN
cord-271701-tx0lqgff	259	15	activities	activity	NNS
cord-271701-tx0lqgff	259	16	of	of	IN
cord-271701-tx0lqgff	259	17	nps8	nps8	NN
cord-271701-tx0lqgff	259	18	,	,	,
cord-271701-tx0lqgff	259	19	His	-PRON-	PRP$
cord-271701-tx0lqgff	259	20	-	-	HYPH
cord-271701-tx0lqgff	259	21	nsp8	nsp8	NN
cord-271701-tx0lqgff	259	22	and	and	CC
cord-271701-tx0lqgff	259	23	nsp7	nsp7	NN
cord-271701-tx0lqgff	259	24	-	-	HYPH
cord-271701-tx0lqgff	259	25	8-His	8-his	NN
cord-271701-tx0lqgff	259	26	.	.	.
cord-271701-tx0lqgff	260	1	(	(	-LRB-
cord-271701-tx0lqgff	260	2	E	E	NNP
cord-271701-tx0lqgff	260	3	)	)	-RRB-
cord-271701-tx0lqgff	260	4	Elution	Elution	NNP
cord-271701-tx0lqgff	260	5	profile	profile	NN
cord-271701-tx0lqgff	260	6	of	of	IN
cord-271701-tx0lqgff	260	7	the	the	DT
cord-271701-tx0lqgff	260	8	nsp7	nsp7	JJ
cord-271701-tx0lqgff	260	9	-	-	HYPH
cord-271701-tx0lqgff	260	10	8-His	8-his	JJ
cord-271701-tx0lqgff	260	11	fusion	fusion	NN
cord-271701-tx0lqgff	260	12	protein	protein	NN
cord-271701-tx0lqgff	260	13	relative	relative	JJ
cord-271701-tx0lqgff	260	14	to	to	IN
cord-271701-tx0lqgff	260	15	nsp8-His	nsp8-His	NNP
cord-271701-tx0lqgff	260	16	.	.	.
cord-271701-tx0lqgff	261	1	(	(	-LRB-
cord-271701-tx0lqgff	261	2	F	F	NNP
cord-271701-tx0lqgff	261	3	)	)	-RRB-
cord-271701-tx0lqgff	261	4	Primer	primer	NN
cord-271701-tx0lqgff	261	5	-	-	HYPH
cord-271701-tx0lqgff	261	6	extension	extension	NN
cord-271701-tx0lqgff	261	7	activities	activity	NNS
cord-271701-tx0lqgff	261	8	of	of	IN
cord-271701-tx0lqgff	261	9	putative	putative	JJ
cord-271701-tx0lqgff	261	10	cleavage	cleavage	NN
cord-271701-tx0lqgff	261	11	intermediate	intermediate	JJ
cord-271701-tx0lqgff	261	12	nsp7	nsp7	NNP
cord-271701-tx0lqgff	261	13	-	-	HYPH
cord-271701-tx0lqgff	261	14	8	8	CD
cord-271701-tx0lqgff	261	15	on	on	IN
cord-271701-tx0lqgff	261	16	the	the	DT
cord-271701-tx0lqgff	261	17	U	U	NNP
cord-271701-tx0lqgff	261	18	20	20	CD
cord-271701-tx0lqgff	261	19	template	template	NN
cord-271701-tx0lqgff	261	20	(	(	-LRB-
cord-271701-tx0lqgff	261	21	see	see	VB
cord-271701-tx0lqgff	261	22	Figure	Figure	NNP
cord-271701-tx0lqgff	261	23	4	4	CD
cord-271701-tx0lqgff	261	24	and	and	CC
cord-271701-tx0lqgff	261	25	Table	Table	NNP
cord-271701-tx0lqgff	261	26	1	1	CD
cord-271701-tx0lqgff	261	27	)	)	-RRB-
cord-271701-tx0lqgff	261	28	.	.	.
cord-271701-tx0lqgff	262	1	and	and	CC
cord-271701-tx0lqgff	262	2	D52	D52	NNP
cord-271701-tx0lqgff	262	3	in	in	IN
cord-271701-tx0lqgff	262	4	SARS	SARS	NNP
cord-271701-tx0lqgff	262	5	-	-	HYPH
cord-271701-tx0lqgff	262	6	CoV	CoV	NNP
cord-271701-tx0lqgff	262	7	,	,	,
cord-271701-tx0lqgff	262	8	abolished	abolish	VBD
cord-271701-tx0lqgff	262	9	RdRp	RdRp	NNP
cord-271701-tx0lqgff	262	10	activity	activity	NN
cord-271701-tx0lqgff	262	11	,	,	,
cord-271701-tx0lqgff	262	12	whereas	whereas	IN
cord-271701-tx0lqgff	262	13	mutation	mutation	NN
cord-271701-tx0lqgff	262	14	of	of	IN
cord-271701-tx0lqgff	262	15	the	the	DT
cord-271701-tx0lqgff	262	16	C	C	NNP
cord-271701-tx0lqgff	262	17	-	-	HYPH
cord-271701-tx0lqgff	262	18	terminal	terminal	NN
cord-271701-tx0lqgff	262	19	motif	motif	NN
cord-271701-tx0lqgff	262	20	,	,	,
cord-271701-tx0lqgff	262	21	including	include	VBG
cord-271701-tx0lqgff	262	22	SARS	SARS	NNP
cord-271701-tx0lqgff	262	23	-	-	HYPH
cord-271701-tx0lqgff	262	24	CoV	cov	NN
cord-271701-tx0lqgff	262	25	residues	residue	NNS
cord-271701-tx0lqgff	262	26	D161	D161	NNP
cord-271701-tx0lqgff	262	27	and	and	CC
cord-271701-tx0lqgff	262	28	D163	D163	NNP
cord-271701-tx0lqgff	262	29	,	,	,
cord-271701-tx0lqgff	262	30	did	do	VBD
cord-271701-tx0lqgff	262	31	not	not	RB
cord-271701-tx0lqgff	262	32	affect	affect	VB
cord-271701-tx0lqgff	262	33	polymerase	polymerase	NN
cord-271701-tx0lqgff	262	34	activity	activity	NN
cord-271701-tx0lqgff	262	35	(	(	-LRB-
cord-271701-tx0lqgff	262	36	Figure	figure	NN
cord-271701-tx0lqgff	262	37	5	5	CD
cord-271701-tx0lqgff	262	38	)	)	-RRB-
cord-271701-tx0lqgff	262	39	.	.	.
cord-271701-tx0lqgff	263	1	Given	give	VBN
cord-271701-tx0lqgff	263	2	the	the	DT
cord-271701-tx0lqgff	263	3	general	general	JJ
cord-271701-tx0lqgff	263	4	importance	importance	NN
cord-271701-tx0lqgff	263	5	of	of	IN
cord-271701-tx0lqgff	263	6	acidic	acidic	JJ
cord-271701-tx0lqgff	263	7	residues	residue	NNS
cord-271701-tx0lqgff	263	8	for	for	IN
cord-271701-tx0lqgff	263	9	metal	metal	NN
cord-271701-tx0lqgff	263	10	-	-	HYPH
cord-271701-tx0lqgff	263	11	ion	ion	NN
cord-271701-tx0lqgff	263	12	coordination	coordination	NN
cord-271701-tx0lqgff	263	13	in	in	IN
cord-271701-tx0lqgff	263	14	polymerase	polymerase	NN
cord-271701-tx0lqgff	263	15	active	active	JJ
cord-271701-tx0lqgff	263	16	sites	site	NNS
cord-271701-tx0lqgff	263	17	(	(	-LRB-
cord-271701-tx0lqgff	263	18	3	3	CD
cord-271701-tx0lqgff	263	19	)	)	-RRB-
cord-271701-tx0lqgff	263	20	(	(	-LRB-
cord-271701-tx0lqgff	263	21	4	4	LS
cord-271701-tx0lqgff	263	22	)	)	-RRB-
cord-271701-tx0lqgff	263	23	(	(	-LRB-
cord-271701-tx0lqgff	263	24	5	5	LS
cord-271701-tx0lqgff	263	25	)	)	-RRB-
cord-271701-tx0lqgff	263	26	22	22	CD
cord-271701-tx0lqgff	263	27	)	)	-RRB-
cord-271701-tx0lqgff	263	28	and	and	CC
cord-271701-tx0lqgff	263	29	the	the	DT
cord-271701-tx0lqgff	263	30	D	D	NNP
cord-271701-tx0lqgff	263	31	/	/	SYM
cord-271701-tx0lqgff	263	32	ExD	ExD	NNP
cord-271701-tx0lqgff	263	33	/	/	SYM
cord-271701-tx0lqgff	263	34	E	e	NN
cord-271701-tx0lqgff	263	35	consensus	consensus	NN
cord-271701-tx0lqgff	263	36	sequence	sequence	NN
cord-271701-tx0lqgff	263	37	in	in	IN
cord-271701-tx0lqgff	263	38	coronaviruses	coronaviruse	NNS
cord-271701-tx0lqgff	263	39	at	at	IN
cord-271701-tx0lqgff	263	40	positions	position	NNS
cord-271701-tx0lqgff	263	41	50	50	CD
cord-271701-tx0lqgff	263	42	-	-	SYM
cord-271701-tx0lqgff	263	43	52	52	CD
cord-271701-tx0lqgff	263	44	,	,	,
cord-271701-tx0lqgff	263	45	we	-PRON-	PRP
cord-271701-tx0lqgff	263	46	now	now	RB
cord-271701-tx0lqgff	263	47	postulate	postulate	VBP
cord-271701-tx0lqgff	263	48	that	that	IN
cord-271701-tx0lqgff	263	49	these	these	DT
cord-271701-tx0lqgff	263	50	residues	residue	NNS
cord-271701-tx0lqgff	263	51	are	be	VBP
cord-271701-tx0lqgff	263	52	part	part	NN
cord-271701-tx0lqgff	263	53	of	of	IN
cord-271701-tx0lqgff	263	54	the	the	DT
cord-271701-tx0lqgff	263	55	Mg	Mg	NNP
cord-271701-tx0lqgff	263	56	2	2	CD
cord-271701-tx0lqgff	263	57	+	+	SYM
cord-271701-tx0lqgff	263	58	-binding	-binde	VBG
cord-271701-tx0lqgff	263	59	active	active	JJ
cord-271701-tx0lqgff	263	60	site	site	NN
cord-271701-tx0lqgff	263	61	in	in	IN
cord-271701-tx0lqgff	263	62	spite	spite	NN
cord-271701-tx0lqgff	263	63	of	of	IN
cord-271701-tx0lqgff	263	64	the	the	DT
cord-271701-tx0lqgff	263	65	more	more	RBR
cord-271701-tx0lqgff	263	66	conserved	conserved	JJ
cord-271701-tx0lqgff	263	67	nature	nature	NN
cord-271701-tx0lqgff	263	68	of	of	IN
cord-271701-tx0lqgff	263	69	D161	d161	NN
cord-271701-tx0lqgff	263	70	and	and	CC
cord-271701-tx0lqgff	263	71	D163	d163	NN
cord-271701-tx0lqgff	263	72	(	(	-LRB-
cord-271701-tx0lqgff	263	73	Figure	figure	NN
cord-271701-tx0lqgff	263	74	5	5	CD
cord-271701-tx0lqgff	263	75	)	)	-RRB-
cord-271701-tx0lqgff	263	76	,	,	,
cord-271701-tx0lqgff	263	77	and	and	CC
cord-271701-tx0lqgff	263	78	their	-PRON-	PRP$
cord-271701-tx0lqgff	263	79	position	position	NN
cord-271701-tx0lqgff	263	80	in	in	IN
cord-271701-tx0lqgff	263	81	the	the	DT
cord-271701-tx0lqgff	263	82	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	263	83	+	+	SYM
cord-271701-tx0lqgff	263	84	8	8	CD
cord-271701-tx0lqgff	263	85	)	)	-RRB-
cord-271701-tx0lqgff	263	86	structure	structure	NN
cord-271701-tx0lqgff	263	87	(	(	-LRB-
cord-271701-tx0lqgff	263	88	see	see	VB
cord-271701-tx0lqgff	263	89	below	below	RB
cord-271701-tx0lqgff	263	90	for	for	IN
cord-271701-tx0lqgff	263	91	further	further	JJ
cord-271701-tx0lqgff	263	92	discussion	discussion	NN
cord-271701-tx0lqgff	263	93	)	)	-RRB-
cord-271701-tx0lqgff	263	94	.	.	.
cord-271701-tx0lqgff	264	1	Secondly	secondly	RB
cord-271701-tx0lqgff	264	2	,	,	,
cord-271701-tx0lqgff	264	3	the	the	DT
cord-271701-tx0lqgff	264	4	presence	presence	NN
cord-271701-tx0lqgff	264	5	of	of	IN
cord-271701-tx0lqgff	264	6	N	n	CD
cord-271701-tx0lqgff	264	7	-	-	HYPH
cord-271701-tx0lqgff	264	8	terminal	terminal	NN
cord-271701-tx0lqgff	264	9	extensions	extension	NNS
cord-271701-tx0lqgff	264	10	other	other	JJ
cord-271701-tx0lqgff	264	11	than	than	IN
cord-271701-tx0lqgff	264	12	nsp7	nsp7	NNP
cord-271701-tx0lqgff	264	13	,	,	,
cord-271701-tx0lqgff	264	14	such	such	JJ
cord-271701-tx0lqgff	264	15	as	as	IN
cord-271701-tx0lqgff	264	16	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	264	17	and	and	CC
cord-271701-tx0lqgff	264	18	His	-PRON-	PRP$
cord-271701-tx0lqgff	264	19	6	6	CD
cord-271701-tx0lqgff	264	20	,	,	,
cord-271701-tx0lqgff	264	21	severely	severely	RB
cord-271701-tx0lqgff	264	22	affected	affect	VBD
cord-271701-tx0lqgff	264	23	the	the	DT
cord-271701-tx0lqgff	264	24	primer	primer	NN
cord-271701-tx0lqgff	264	25	extension	extension	NN
cord-271701-tx0lqgff	264	26	activity	activity	NN
cord-271701-tx0lqgff	264	27	of	of	IN
cord-271701-tx0lqgff	264	28	nsp8	nsp8	NN
cord-271701-tx0lqgff	264	29	(	(	-LRB-
cord-271701-tx0lqgff	264	30	Figure	Figure	NNP
cord-271701-tx0lqgff	264	31	7	7	CD
cord-271701-tx0lqgff	264	32	)	)	-RRB-
cord-271701-tx0lqgff	264	33	,	,	,
cord-271701-tx0lqgff	264	34	potentially	potentially	RB
cord-271701-tx0lqgff	264	35	by	by	IN
cord-271701-tx0lqgff	264	36	changing	change	VBG
cord-271701-tx0lqgff	264	37	its	-PRON-	PRP$
cord-271701-tx0lqgff	264	38	oligomeric	oligomeric	JJ
cord-271701-tx0lqgff	264	39	state	state	NN
cord-271701-tx0lqgff	264	40	(	(	-LRB-
cord-271701-tx0lqgff	264	41	Figure	figure	NN
cord-271701-tx0lqgff	264	42	2	2	CD
cord-271701-tx0lqgff	264	43	)	)	-RRB-
cord-271701-tx0lqgff	264	44	.	.	.
cord-271701-tx0lqgff	265	1	However	however	RB
cord-271701-tx0lqgff	265	2	,	,	,
cord-271701-tx0lqgff	265	3	the	the	DT
cord-271701-tx0lqgff	265	4	relatively	relatively	RB
cord-271701-tx0lqgff	265	5	strong	strong	JJ
cord-271701-tx0lqgff	265	6	activity	activity	NN
cord-271701-tx0lqgff	265	7	of	of	IN
cord-271701-tx0lqgff	265	8	nsp7	nsp7	JJ
cord-271701-tx0lqgff	265	9	-	-	HYPH
cord-271701-tx0lqgff	265	10	8	8	CD
cord-271701-tx0lqgff	265	11	(	(	-LRB-
cord-271701-tx0lqgff	265	12	Figure	Figure	NNP
cord-271701-tx0lqgff	265	13	7	7	CD
cord-271701-tx0lqgff	265	14	)	)	-RRB-
cord-271701-tx0lqgff	265	15	,	,	,
cord-271701-tx0lqgff	265	16	a	a	DT
cord-271701-tx0lqgff	265	17	potential	potential	NN
cord-271701-tx0lqgff	265	18	naturally	naturally	RB
cord-271701-tx0lqgff	265	19	occurring	occur	VBG
cord-271701-tx0lqgff	265	20	replicase	replicase	NN
cord-271701-tx0lqgff	265	21	processing	processing	NN
cord-271701-tx0lqgff	265	22	intermediate	intermediate	JJ
cord-271701-tx0lqgff	265	23	,	,	,
cord-271701-tx0lqgff	265	24	implies	imply	VBZ
cord-271701-tx0lqgff	265	25	that	that	IN
cord-271701-tx0lqgff	265	26	nsp8	nsp8	NN
cord-271701-tx0lqgff	265	27	's	's	POS
cord-271701-tx0lqgff	265	28	activity	activity	NN
cord-271701-tx0lqgff	265	29	is	be	VBZ
cord-271701-tx0lqgff	265	30	unlikely	unlikely	JJ
cord-271701-tx0lqgff	265	31	to	to	TO
cord-271701-tx0lqgff	265	32	be	be	VB
cord-271701-tx0lqgff	265	33	directly	directly	RB
cord-271701-tx0lqgff	265	34	controlled	control	VBN
cord-271701-tx0lqgff	265	35	by	by	IN
cord-271701-tx0lqgff	265	36	an	an	DT
cord-271701-tx0lqgff	265	37	N	n	CD
cord-271701-tx0lqgff	265	38	-	-	HYPH
cord-271701-tx0lqgff	265	39	terminal	terminal	NN
cord-271701-tx0lqgff	265	40	cleavage	cleavage	NN
cord-271701-tx0lqgff	265	41	event	event	NN
cord-271701-tx0lqgff	265	42	,	,	,
cord-271701-tx0lqgff	265	43	as	as	IN
cord-271701-tx0lqgff	265	44	was	be	VBD
cord-271701-tx0lqgff	265	45	observed	observe	VBN
cord-271701-tx0lqgff	265	46	for	for	IN
cord-271701-tx0lqgff	265	47	,	,	,
cord-271701-tx0lqgff	265	48	e.g.	e.g.	RB
cord-271701-tx0lqgff	265	49	the	the	DT
cord-271701-tx0lqgff	265	50	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	265	51	polymerase	polymerase	NN
cord-271701-tx0lqgff	265	52	(	(	-LRB-
cord-271701-tx0lqgff	265	53	29	29	CD
cord-271701-tx0lqgff	265	54	)	)	-RRB-
cord-271701-tx0lqgff	265	55	.	.	.
cord-271701-tx0lqgff	266	1	In	in	IN
cord-271701-tx0lqgff	266	2	addition	addition	NN
cord-271701-tx0lqgff	266	3	,	,	,
cord-271701-tx0lqgff	266	4	these	these	DT
cord-271701-tx0lqgff	266	5	observations	observation	NNS
cord-271701-tx0lqgff	266	6	suggest	suggest	VBP
cord-271701-tx0lqgff	266	7	that	that	IN
cord-271701-tx0lqgff	266	8	a	a	DT
cord-271701-tx0lqgff	266	9	more	more	RBR
cord-271701-tx0lqgff	266	10	diverse	diverse	JJ
cord-271701-tx0lqgff	266	11	array	array	NN
cord-271701-tx0lqgff	266	12	of	of	IN
cord-271701-tx0lqgff	266	13	nsp8-containing	nsp8-containing	NNP
cord-271701-tx0lqgff	266	14	RdRps	RdRps	NNP
cord-271701-tx0lqgff	266	15	may	may	MD
cord-271701-tx0lqgff	266	16	be	be	VB
cord-271701-tx0lqgff	266	17	involved	involve	VBN
cord-271701-tx0lqgff	266	18	in	in	IN
cord-271701-tx0lqgff	266	19	CoV	cov	NN
cord-271701-tx0lqgff	266	20	replication	replication	NN
cord-271701-tx0lqgff	266	21	and	and	CC
cord-271701-tx0lqgff	266	22	transcription	transcription	NN
cord-271701-tx0lqgff	266	23	.	.	.
cord-271701-tx0lqgff	267	1	Comparing	compare	VBG
cord-271701-tx0lqgff	267	2	our	-PRON-	PRP$
cord-271701-tx0lqgff	267	3	data	datum	NNS
cord-271701-tx0lqgff	267	4	against	against	IN
cord-271701-tx0lqgff	267	5	the	the	DT
cord-271701-tx0lqgff	267	6	background	background	NN
cord-271701-tx0lqgff	267	7	of	of	IN
cord-271701-tx0lqgff	267	8	the	the	DT
cord-271701-tx0lqgff	267	9	previously	previously	RB
cord-271701-tx0lqgff	267	10	published	publish	VBN
cord-271701-tx0lqgff	267	11	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	267	12	+	+	SYM
cord-271701-tx0lqgff	267	13	8	8	CD
cord-271701-tx0lqgff	267	14	)	)	-RRB-
cord-271701-tx0lqgff	267	15	structure	structure	NN
cord-271701-tx0lqgff	267	16	(	(	-LRB-
cord-271701-tx0lqgff	267	17	13	13	CD
cord-271701-tx0lqgff	267	18	)	)	-RRB-
cord-271701-tx0lqgff	267	19	,	,	,
cord-271701-tx0lqgff	267	20	we	-PRON-	PRP
cord-271701-tx0lqgff	267	21	made	make	VBD
cord-271701-tx0lqgff	267	22	four	four	CD
cord-271701-tx0lqgff	267	23	main	main	JJ
cord-271701-tx0lqgff	267	24	observations	observation	NNS
cord-271701-tx0lqgff	267	25	.	.	.
cord-271701-tx0lqgff	268	1	Firstly	firstly	RB
cord-271701-tx0lqgff	268	2	,	,	,
cord-271701-tx0lqgff	268	3	we	-PRON-	PRP
cord-271701-tx0lqgff	268	4	note	note	VBP
cord-271701-tx0lqgff	268	5	that	that	IN
cord-271701-tx0lqgff	268	6	in	in	IN
cord-271701-tx0lqgff	268	7	the	the	DT
cord-271701-tx0lqgff	268	8	published	published	JJ
cord-271701-tx0lqgff	268	9	nsp(7	nsp(7	CD
cord-271701-tx0lqgff	268	10	+	+	SYM
cord-271701-tx0lqgff	268	11	8	8	CD
cord-271701-tx0lqgff	268	12	)	)	-RRB-
cord-271701-tx0lqgff	268	13	crystal	crystal	NN
cord-271701-tx0lqgff	268	14	structure	structure	NN
cord-271701-tx0lqgff	268	15	four	four	CD
cord-271701-tx0lqgff	268	16	of	of	IN
cord-271701-tx0lqgff	268	17	the	the	DT
cord-271701-tx0lqgff	268	18	eight	eight	CD
cord-271701-tx0lqgff	268	19	N	n	CD
cord-271701-tx0lqgff	268	20	-	-	HYPH
cord-271701-tx0lqgff	268	21	terminal	terminal	NN
cord-271701-tx0lqgff	268	22	D	D	NNP
cord-271701-tx0lqgff	268	23	/	/	SYM
cord-271701-tx0lqgff	268	24	ExD	ExD	NNP
cord-271701-tx0lqgff	268	25	/	/	SYM
cord-271701-tx0lqgff	268	26	E	e	NN
cord-271701-tx0lqgff	268	27	motifs	motif	NNS
cord-271701-tx0lqgff	268	28	in	in	IN
cord-271701-tx0lqgff	268	29	the	the	DT
cord-271701-tx0lqgff	268	30	complex	complex	NN
cord-271701-tx0lqgff	268	31	reside	reside	VBP
cord-271701-tx0lqgff	268	32	at	at	IN
cord-271701-tx0lqgff	268	33	the	the	DT
cord-271701-tx0lqgff	268	34	border	border	NN
cord-271701-tx0lqgff	268	35	of	of	IN
cord-271701-tx0lqgff	268	36	partially	partially	RB
cord-271701-tx0lqgff	268	37	unresolved	unresolved	JJ
cord-271701-tx0lqgff	268	38	N	n	CD
cord-271701-tx0lqgff	268	39	-	-	HYPH
cord-271701-tx0lqgff	268	40	terminal	terminal	NN
cord-271701-tx0lqgff	268	41	nsp8	nsp8	JJ
cord-271701-tx0lqgff	268	42	domains	domain	NNS
cord-271701-tx0lqgff	268	43	,	,	,
cord-271701-tx0lqgff	268	44	where	where	WRB
cord-271701-tx0lqgff	268	45	the	the	DT
cord-271701-tx0lqgff	268	46	coordinates	coordinate	NNS
cord-271701-tx0lqgff	268	47	of	of	IN
cord-271701-tx0lqgff	268	48	up	up	IN
cord-271701-tx0lqgff	268	49	to	to	TO
cord-271701-tx0lqgff	268	50	49	49	CD
cord-271701-tx0lqgff	268	51	nsp8	nsp8	JJ
cord-271701-tx0lqgff	268	52	residues	residue	NNS
cord-271701-tx0lqgff	268	53	and	and	CC
cord-271701-tx0lqgff	268	54	5	5	CD
cord-271701-tx0lqgff	268	55	exogenous	exogenous	JJ
cord-271701-tx0lqgff	268	56	amino	amino	NN
cord-271701-tx0lqgff	268	57	acids	acid	NNS
cord-271701-tx0lqgff	268	58	derived	derive	VBN
cord-271701-tx0lqgff	268	59	from	from	IN
cord-271701-tx0lqgff	268	60	the	the	DT
cord-271701-tx0lqgff	268	61	removed	remove	VBN
cord-271701-tx0lqgff	268	62	GST	gst	NN
cord-271701-tx0lqgff	268	63	fusion	fusion	NN
cord-271701-tx0lqgff	268	64	partner	partner	NN
cord-271701-tx0lqgff	268	65	were	be	VBD
cord-271701-tx0lqgff	268	66	not	not	RB
cord-271701-tx0lqgff	268	67	determined	determine	VBN
cord-271701-tx0lqgff	268	68	.	.	.
cord-271701-tx0lqgff	269	1	In	in	IN
cord-271701-tx0lqgff	269	2	light	light	NN
cord-271701-tx0lqgff	269	3	of	of	IN
cord-271701-tx0lqgff	269	4	our	-PRON-	PRP$
cord-271701-tx0lqgff	269	5	own	own	JJ
cord-271701-tx0lqgff	269	6	finding	finding	NN
cord-271701-tx0lqgff	269	7	that	that	IN
cord-271701-tx0lqgff	269	8	unnatural	unnatural	JJ
cord-271701-tx0lqgff	269	9	N	n	CD
cord-271701-tx0lqgff	269	10	-	-	HYPH
cord-271701-tx0lqgff	269	11	terminal	terminal	NN
cord-271701-tx0lqgff	269	12	extensions	extension	NNS
cord-271701-tx0lqgff	269	13	severely	severely	RB
cord-271701-tx0lqgff	269	14	impair	impair	VBP
cord-271701-tx0lqgff	269	15	nsp8	nsp8	NN
cord-271701-tx0lqgff	269	16	's	's	POS
cord-271701-tx0lqgff	269	17	RdRp	rdrp	NN
cord-271701-tx0lqgff	269	18	activity	activity	NN
cord-271701-tx0lqgff	269	19	(	(	-LRB-
cord-271701-tx0lqgff	269	20	Figure	figure	NN
cord-271701-tx0lqgff	269	21	5	5	CD
cord-271701-tx0lqgff	269	22	)	)	-RRB-
cord-271701-tx0lqgff	269	23	,	,	,
cord-271701-tx0lqgff	269	24	this	this	DT
cord-271701-tx0lqgff	269	25	suggests	suggest	VBZ
cord-271701-tx0lqgff	269	26	that	that	IN
cord-271701-tx0lqgff	269	27	the	the	DT
cord-271701-tx0lqgff	269	28	published	publish	VBN
cord-271701-tx0lqgff	269	29	crystal	crystal	NN
cord-271701-tx0lqgff	269	30	structure	structure	NN
cord-271701-tx0lqgff	269	31	may	may	MD
cord-271701-tx0lqgff	269	32	not	not	RB
cord-271701-tx0lqgff	269	33	represent	represent	VB
cord-271701-tx0lqgff	269	34	an	an	DT
cord-271701-tx0lqgff	269	35	active	active	JJ
cord-271701-tx0lqgff	269	36	conformation	conformation	NN
cord-271701-tx0lqgff	269	37	of	of	IN
cord-271701-tx0lqgff	269	38	the	the	DT
cord-271701-tx0lqgff	269	39	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	269	40	+	+	SYM
cord-271701-tx0lqgff	269	41	8	8	CD
cord-271701-tx0lqgff	269	42	)	)	-RRB-
cord-271701-tx0lqgff	269	43	polymerase	polymerase	NN
cord-271701-tx0lqgff	269	44	.	.	.
cord-271701-tx0lqgff	270	1	Secondly	secondly	RB
cord-271701-tx0lqgff	270	2	,	,	,
cord-271701-tx0lqgff	270	3	we	-PRON-	PRP
cord-271701-tx0lqgff	270	4	observe	observe	VBP
cord-271701-tx0lqgff	270	5	that	that	IN
cord-271701-tx0lqgff	270	6	residues	residue	VBZ
cord-271701-tx0lqgff	270	7	D50	D50	NNP
cord-271701-tx0lqgff	270	8	and	and	CC
cord-271701-tx0lqgff	270	9	D52	D52	NNP
cord-271701-tx0lqgff	270	10	,	,	,
cord-271701-tx0lqgff	270	11	which	which	WDT
cord-271701-tx0lqgff	270	12	are	be	VBP
cord-271701-tx0lqgff	270	13	both	both	DT
cord-271701-tx0lqgff	270	14	crucial	crucial	JJ
cord-271701-tx0lqgff	270	15	for	for	IN
cord-271701-tx0lqgff	270	16	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	270	17	+	+	SYM
cord-271701-tx0lqgff	270	18	8	8	CD
cord-271701-tx0lqgff	270	19	)	)	-RRB-
cord-271701-tx0lqgff	270	20	activity	activity	NN
cord-271701-tx0lqgff	270	21	,	,	,
cord-271701-tx0lqgff	270	22	are	be	VBP
cord-271701-tx0lqgff	270	23	residing	reside	VBG
cord-271701-tx0lqgff	270	24	in	in	IN
cord-271701-tx0lqgff	270	25	an	an	DT
cord-271701-tx0lqgff	270	26	a	a	DT
cord-271701-tx0lqgff	270	27	-	-	HYPH
cord-271701-tx0lqgff	270	28	helix	helix	NN
cord-271701-tx0lqgff	270	29	in	in	IN
cord-271701-tx0lqgff	270	30	the	the	DT
cord-271701-tx0lqgff	270	31	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	270	32	+	+	SYM
cord-271701-tx0lqgff	270	33	8	8	CD
cord-271701-tx0lqgff	270	34	)	)	-RRB-
cord-271701-tx0lqgff	270	35	structure	structure	NN
cord-271701-tx0lqgff	270	36	(	(	-LRB-
cord-271701-tx0lqgff	270	37	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	270	38	Figure	Figure	NNP
cord-271701-tx0lqgff	270	39	 	 	_SP
cord-271701-tx0lqgff	270	40	S5	S5	NNP
cord-271701-tx0lqgff	270	41	)	)	-RRB-
cord-271701-tx0lqgff	270	42	,	,	,
cord-271701-tx0lqgff	270	43	whereas	whereas	IN
cord-271701-tx0lqgff	270	44	in	in	IN
cord-271701-tx0lqgff	270	45	canonical	canonical	JJ
cord-271701-tx0lqgff	270	46	primases	primase	NNS
cord-271701-tx0lqgff	270	47	and	and	CC
cord-271701-tx0lqgff	270	48	polymerases	polymerase	NNS
cord-271701-tx0lqgff	270	49	,	,	,
cord-271701-tx0lqgff	270	50	the	the	DT
cord-271701-tx0lqgff	270	51	catalytic	catalytic	JJ
cord-271701-tx0lqgff	270	52	centre	centre	NN
cord-271701-tx0lqgff	270	53	is	be	VBZ
cord-271701-tx0lqgff	270	54	preferentially	preferentially	RB
cord-271701-tx0lqgff	270	55	located	locate	VBN
cord-271701-tx0lqgff	270	56	on	on	IN
cord-271701-tx0lqgff	270	57	b	b	NN
cord-271701-tx0lqgff	270	58	-	-	HYPH
cord-271701-tx0lqgff	270	59	strands	strand	NNS
cord-271701-tx0lqgff	270	60	or	or	CC
cord-271701-tx0lqgff	270	61	turns	turn	NNS
cord-271701-tx0lqgff	270	62	(	(	-LRB-
cord-271701-tx0lqgff	270	63	12	12	CD
cord-271701-tx0lqgff	270	64	,	,	,
cord-271701-tx0lqgff	270	65	22	22	CD
cord-271701-tx0lqgff	270	66	)	)	-RRB-
cord-271701-tx0lqgff	270	67	.	.	.
cord-271701-tx0lqgff	271	1	Thirdly	thirdly	RB
cord-271701-tx0lqgff	271	2	,	,	,
cord-271701-tx0lqgff	271	3	we	-PRON-	PRP
cord-271701-tx0lqgff	271	4	note	note	VBP
cord-271701-tx0lqgff	271	5	that	that	IN
cord-271701-tx0lqgff	271	6	Mg	Mg	NNP
cord-271701-tx0lqgff	271	7	2	2	CD
cord-271701-tx0lqgff	271	8	+	+	SYM
cord-271701-tx0lqgff	271	9	was	be	VBD
cord-271701-tx0lqgff	271	10	lacking	lack	VBG
cord-271701-tx0lqgff	271	11	from	from	IN
cord-271701-tx0lqgff	271	12	the	the	DT
cord-271701-tx0lqgff	271	13	published	publish	VBN
cord-271701-tx0lqgff	271	14	nsp(7	nsp(7	CD
cord-271701-tx0lqgff	271	15	+	+	SYM
cord-271701-tx0lqgff	271	16	8	8	CD
cord-271701-tx0lqgff	271	17	)	)	-RRB-
cord-271701-tx0lqgff	271	18	crystal	crystal	NN
cord-271701-tx0lqgff	271	19	structure	structure	NN
cord-271701-tx0lqgff	271	20	(	(	-LRB-
cord-271701-tx0lqgff	271	21	13	13	CD
cord-271701-tx0lqgff	271	22	)	)	-RRB-
cord-271701-tx0lqgff	271	23	,	,	,
cord-271701-tx0lqgff	271	24	even	even	RB
cord-271701-tx0lqgff	271	25	though	though	IN
cord-271701-tx0lqgff	271	26	it	-PRON-	PRP
cord-271701-tx0lqgff	271	27	is	be	VBZ
cord-271701-tx0lqgff	271	28	required	require	VBN
cord-271701-tx0lqgff	271	29	for	for	IN
cord-271701-tx0lqgff	271	30	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	271	31	+	+	SYM
cord-271701-tx0lqgff	271	32	8	8	CD
cord-271701-tx0lqgff	271	33	)	)	-RRB-
cord-271701-tx0lqgff	271	34	activity	activity	NN
cord-271701-tx0lqgff	271	35	.	.	.
cord-271701-tx0lqgff	272	1	Fourthly	fourthly	RB
cord-271701-tx0lqgff	272	2	and	and	CC
cord-271701-tx0lqgff	272	3	last	last	JJ
cord-271701-tx0lqgff	272	4	,	,	,
cord-271701-tx0lqgff	272	5	we	-PRON-	PRP
cord-271701-tx0lqgff	272	6	observe	observe	VBP
cord-271701-tx0lqgff	272	7	that	that	IN
cord-271701-tx0lqgff	272	8	a	a	DT
cord-271701-tx0lqgff	272	9	1:1	1:1	CD
cord-271701-tx0lqgff	272	10	ratio	ratio	NN
cord-271701-tx0lqgff	272	11	of	of	IN
cord-271701-tx0lqgff	272	12	nsp7	nsp7	NN
cord-271701-tx0lqgff	272	13	:	:	:
cord-271701-tx0lqgff	272	14	nsp8	nsp8	NN
cord-271701-tx0lqgff	272	15	is	be	VBZ
cord-271701-tx0lqgff	272	16	sufficient	sufficient	JJ
cord-271701-tx0lqgff	272	17	to	to	TO
cord-271701-tx0lqgff	272	18	capture	capture	VB
cord-271701-tx0lqgff	272	19	all	all	DT
cord-271701-tx0lqgff	272	20	nsp8	nsp8	NN
cord-271701-tx0lqgff	272	21	in	in	IN
cord-271701-tx0lqgff	272	22	a	a	DT
cord-271701-tx0lqgff	272	23	higher	high	JJR
cord-271701-tx0lqgff	272	24	molecular	molecular	JJ
cord-271701-tx0lqgff	272	25	weight	weight	NN
cord-271701-tx0lqgff	272	26	complex	complex	NN
cord-271701-tx0lqgff	272	27	(	(	-LRB-
cord-271701-tx0lqgff	272	28	Figure	figure	NN
cord-271701-tx0lqgff	272	29	2F	2f	NN
cord-271701-tx0lqgff	272	30	)	)	-RRB-
cord-271701-tx0lqgff	272	31	whereas	whereas	IN
cord-271701-tx0lqgff	272	32	previously	previously	RB
cord-271701-tx0lqgff	272	33	a	a	DT
cord-271701-tx0lqgff	272	34	2:1	2:1	CD
cord-271701-tx0lqgff	272	35	ratio	ratio	NN
cord-271701-tx0lqgff	272	36	was	be	VBD
cord-271701-tx0lqgff	272	37	required	require	VBN
cord-271701-tx0lqgff	272	38	(	(	-LRB-
cord-271701-tx0lqgff	272	39	13	13	CD
cord-271701-tx0lqgff	272	40	)	)	-RRB-
cord-271701-tx0lqgff	272	41	,	,	,
cord-271701-tx0lqgff	272	42	potentially	potentially	RB
cord-271701-tx0lqgff	272	43	due	due	JJ
cord-271701-tx0lqgff	272	44	to	to	IN
cord-271701-tx0lqgff	272	45	the	the	DT
cord-271701-tx0lqgff	272	46	additional	additional	JJ
cord-271701-tx0lqgff	272	47	N	n	CD
cord-271701-tx0lqgff	272	48	-	-	HYPH
cord-271701-tx0lqgff	272	49	terminal	terminal	NN
cord-271701-tx0lqgff	272	50	residues	residue	NNS
cord-271701-tx0lqgff	272	51	that	that	WDT
cord-271701-tx0lqgff	272	52	altered	alter	VBD
cord-271701-tx0lqgff	272	53	the	the	DT
cord-271701-tx0lqgff	272	54	dynamics	dynamic	NNS
cord-271701-tx0lqgff	272	55	of	of	IN
cord-271701-tx0lqgff	272	56	complex	complex	JJ
cord-271701-tx0lqgff	272	57	formation	formation	NN
cord-271701-tx0lqgff	272	58	.	.	.
cord-271701-tx0lqgff	273	1	The	the	DT
cord-271701-tx0lqgff	273	2	(	(	-LRB-
cord-271701-tx0lqgff	273	3	functional	functional	JJ
cord-271701-tx0lqgff	273	4	)	)	-RRB-
cord-271701-tx0lqgff	273	5	implications	implication	NNS
cord-271701-tx0lqgff	273	6	of	of	IN
cord-271701-tx0lqgff	273	7	these	these	DT
cord-271701-tx0lqgff	273	8	observations	observation	NNS
cord-271701-tx0lqgff	273	9	are	be	VBP
cord-271701-tx0lqgff	273	10	not	not	RB
cord-271701-tx0lqgff	273	11	clear	clear	JJ
cord-271701-tx0lqgff	273	12	at	at	IN
cord-271701-tx0lqgff	273	13	present	present	JJ
cord-271701-tx0lqgff	273	14	,	,	,
cord-271701-tx0lqgff	273	15	but	but	CC
cord-271701-tx0lqgff	273	16	additional	additional	JJ
cord-271701-tx0lqgff	273	17	structural	structural	JJ
cord-271701-tx0lqgff	273	18	studies	study	NNS
cord-271701-tx0lqgff	273	19	will	will	MD
cord-271701-tx0lqgff	273	20	likely	likely	RB
cord-271701-tx0lqgff	273	21	be	be	VB
cord-271701-tx0lqgff	273	22	required	require	VBN
cord-271701-tx0lqgff	273	23	to	to	TO
cord-271701-tx0lqgff	273	24	address	address	VB
cord-271701-tx0lqgff	273	25	these	these	DT
cord-271701-tx0lqgff	273	26	issues	issue	NNS
cord-271701-tx0lqgff	273	27	in	in	IN
cord-271701-tx0lqgff	273	28	detail	detail	NN
cord-271701-tx0lqgff	273	29	,	,	,
cord-271701-tx0lqgff	273	30	and	and	CC
cord-271701-tx0lqgff	273	31	gain	gain	VB
cord-271701-tx0lqgff	273	32	insights	insight	NNS
cord-271701-tx0lqgff	273	33	that	that	WDT
cord-271701-tx0lqgff	273	34	may	may	MD
cord-271701-tx0lqgff	273	35	aid	aid	VB
cord-271701-tx0lqgff	273	36	in	in	IN
cord-271701-tx0lqgff	273	37	explaining	explain	VBG
cord-271701-tx0lqgff	273	38	the	the	DT
cord-271701-tx0lqgff	273	39	in	in	FW
cord-271701-tx0lqgff	273	40	vitro	vitro	FW
cord-271701-tx0lqgff	273	41	results	result	NNS
cord-271701-tx0lqgff	273	42	presented	present	VBN
cord-271701-tx0lqgff	273	43	here	here	RB
cord-271701-tx0lqgff	273	44	.	.	.
cord-271701-tx0lqgff	274	1	Likely	likely	RB
cord-271701-tx0lqgff	274	2	,	,	,
cord-271701-tx0lqgff	274	3	such	such	JJ
cord-271701-tx0lqgff	274	4	experiments	experiment	NNS
cord-271701-tx0lqgff	274	5	will	will	MD
cord-271701-tx0lqgff	274	6	also	also	RB
cord-271701-tx0lqgff	274	7	offer	offer	VB
cord-271701-tx0lqgff	274	8	further	further	JJ
cord-271701-tx0lqgff	274	9	information	information	NN
cord-271701-tx0lqgff	274	10	regarding	regard	VBG
cord-271701-tx0lqgff	274	11	the	the	DT
cord-271701-tx0lqgff	274	12	residues	residue	NNS
cord-271701-tx0lqgff	274	13	that	that	WDT
cord-271701-tx0lqgff	274	14	are	be	VBP
cord-271701-tx0lqgff	274	15	involved	involve	VBN
cord-271701-tx0lqgff	274	16	in	in	IN
cord-271701-tx0lqgff	274	17	nucleotide	nucleotide	JJ
cord-271701-tx0lqgff	274	18	positioning	positioning	NN
cord-271701-tx0lqgff	274	19	,	,	,
cord-271701-tx0lqgff	274	20	Mg	Mg	NNP
cord-271701-tx0lqgff	274	21	2	2	CD
cord-271701-tx0lqgff	274	22	+	+	CC
cord-271701-tx0lqgff	274	23	coordination	coordination	NN
cord-271701-tx0lqgff	274	24	and	and	CC
cord-271701-tx0lqgff	274	25	RdRp	RdRp	NNP
cord-271701-tx0lqgff	274	26	chemistry	chemistry	NN
cord-271701-tx0lqgff	274	27	.	.	.
cord-271701-tx0lqgff	275	1	In	in	IN
cord-271701-tx0lqgff	275	2	summary	summary	NN
cord-271701-tx0lqgff	275	3	,	,	,
cord-271701-tx0lqgff	275	4	our	-PRON-	PRP$
cord-271701-tx0lqgff	275	5	results	result	NNS
cord-271701-tx0lqgff	275	6	provide	provide	VBP
cord-271701-tx0lqgff	275	7	important	important	JJ
cord-271701-tx0lqgff	275	8	novel	novel	JJ
cord-271701-tx0lqgff	275	9	insights	insight	NNS
cord-271701-tx0lqgff	275	10	into	into	IN
cord-271701-tx0lqgff	275	11	the	the	DT
cord-271701-tx0lqgff	275	12	functionality	functionality	NN
cord-271701-tx0lqgff	275	13	of	of	IN
cord-271701-tx0lqgff	275	14	the	the	DT
cord-271701-tx0lqgff	275	15	SARS	SARS	NNP
cord-271701-tx0lqgff	275	16	-	-	HYPH
cord-271701-tx0lqgff	275	17	CoV	CoV	NNP
cord-271701-tx0lqgff	275	18	hexadecameric	hexadecameric	NNP
cord-271701-tx0lqgff	275	19	nsp(7	nsp(7	NNP
cord-271701-tx0lqgff	275	20	+	+	SYM
cord-271701-tx0lqgff	275	21	8	8	CD
cord-271701-tx0lqgff	275	22	)	)	-RRB-
cord-271701-tx0lqgff	275	23	complex	complex	JJ
cord-271701-tx0lqgff	275	24	and	and	CC
cord-271701-tx0lqgff	275	25	demonstrate	demonstrate	VB
cord-271701-tx0lqgff	275	26	its	-PRON-	PRP$
cord-271701-tx0lqgff	275	27	activity	activity	NN
cord-271701-tx0lqgff	275	28	as	as	IN
cord-271701-tx0lqgff	275	29	an	an	DT
cord-271701-tx0lqgff	275	30	RNA	RNA	NNP
cord-271701-tx0lqgff	275	31	polymerase	polymerase	NN
cord-271701-tx0lqgff	275	32	.	.	.
cord-271701-tx0lqgff	276	1	In	in	IN
cord-271701-tx0lqgff	276	2	addition	addition	NN
cord-271701-tx0lqgff	276	3	,	,	,
cord-271701-tx0lqgff	276	4	our	-PRON-	PRP$
cord-271701-tx0lqgff	276	5	experiments	experiment	NNS
cord-271701-tx0lqgff	276	6	and	and	CC
cord-271701-tx0lqgff	276	7	controls	control	NNS
cord-271701-tx0lqgff	276	8	revealed	reveal	VBD
cord-271701-tx0lqgff	276	9	and	and	CC
cord-271701-tx0lqgff	276	10	address	address	VB
cord-271701-tx0lqgff	276	11	a	a	DT
cord-271701-tx0lqgff	276	12	number	number	NN
cord-271701-tx0lqgff	276	13	of	of	IN
cord-271701-tx0lqgff	276	14	disparities	disparity	NNS
cord-271701-tx0lqgff	276	15	between	between	IN
cord-271701-tx0lqgff	276	16	previous	previous	JJ
cord-271701-tx0lqgff	276	17	claims	claim	NNS
cord-271701-tx0lqgff	276	18	and	and	CC
cord-271701-tx0lqgff	276	19	hypotheses	hypothesis	NNS
cord-271701-tx0lqgff	276	20	(	(	-LRB-
cord-271701-tx0lqgff	276	21	12	12	CD
cord-271701-tx0lqgff	276	22	)	)	-RRB-
cord-271701-tx0lqgff	276	23	,	,	,
cord-271701-tx0lqgff	276	24	and	and	CC
cord-271701-tx0lqgff	276	25	our	-PRON-	PRP$
cord-271701-tx0lqgff	276	26	own	own	JJ
cord-271701-tx0lqgff	276	27	observations	observation	NNS
cord-271701-tx0lqgff	276	28	.	.	.
cord-271701-tx0lqgff	277	1	The	the	DT
cord-271701-tx0lqgff	277	2	'	'	``
cord-271701-tx0lqgff	277	3	primase	primase	NN
cord-271701-tx0lqgff	277	4	hypothesis	hypothesis	NN
cord-271701-tx0lqgff	277	5	'	'	''
cord-271701-tx0lqgff	277	6	previously	previously	RB
cord-271701-tx0lqgff	277	7	formulated	formulate	VBN
cord-271701-tx0lqgff	277	8	by	by	IN
cord-271701-tx0lqgff	277	9	Imbert	Imbert	NNP
cord-271701-tx0lqgff	277	10	and	and	CC
cord-271701-tx0lqgff	277	11	co	co	NNS
cord-271701-tx0lqgff	277	12	-	-	NNS
cord-271701-tx0lqgff	277	13	workers	worker	NNS
cord-271701-tx0lqgff	277	14	(	(	-LRB-
cord-271701-tx0lqgff	277	15	12	12	CD
cord-271701-tx0lqgff	277	16	)	)	-RRB-
cord-271701-tx0lqgff	277	17	remains	remain	VBZ
cord-271701-tx0lqgff	277	18	an	an	DT
cord-271701-tx0lqgff	277	19	intriguing	intriguing	JJ
cord-271701-tx0lqgff	277	20	model	model	NN
cord-271701-tx0lqgff	277	21	to	to	TO
cord-271701-tx0lqgff	277	22	explain	explain	VB
cord-271701-tx0lqgff	277	23	the	the	DT
cord-271701-tx0lqgff	277	24	initiation	initiation	NN
cord-271701-tx0lqgff	277	25	of	of	IN
cord-271701-tx0lqgff	277	26	SARS	SARS	NNP
cord-271701-tx0lqgff	277	27	-	-	HYPH
cord-271701-tx0lqgff	277	28	CoV	CoV	NNP
cord-271701-tx0lqgff	277	29	RNA	RNA	NNP
cord-271701-tx0lqgff	277	30	synthesis	synthesis	NN
cord-271701-tx0lqgff	277	31	and	and	CC
cord-271701-tx0lqgff	277	32	is	be	VBZ
cord-271701-tx0lqgff	277	33	a	a	DT
cord-271701-tx0lqgff	277	34	topic	topic	NN
cord-271701-tx0lqgff	277	35	that	that	WDT
cord-271701-tx0lqgff	277	36	will	will	MD
cord-271701-tx0lqgff	277	37	be	be	VB
cord-271701-tx0lqgff	277	38	addressed	address	VBN
cord-271701-tx0lqgff	277	39	in	in	IN
cord-271701-tx0lqgff	277	40	detail	detail	NN
cord-271701-tx0lqgff	277	41	elsewhere	elsewhere	RB
cord-271701-tx0lqgff	277	42	.	.	.
cord-271701-tx0lqgff	278	1	Nevertheless	nevertheless	RB
cord-271701-tx0lqgff	278	2	,	,	,
cord-271701-tx0lqgff	278	3	based	base	VBN
cord-271701-tx0lqgff	278	4	on	on	IN
cord-271701-tx0lqgff	278	5	the	the	DT
cord-271701-tx0lqgff	278	6	primer	primer	NN
cord-271701-tx0lqgff	278	7	extension	extension	NN
cord-271701-tx0lqgff	278	8	activity	activity	NN
cord-271701-tx0lqgff	278	9	of	of	IN
cord-271701-tx0lqgff	278	10	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	278	11	+	+	SYM
cord-271701-tx0lqgff	278	12	8	8	CD
cord-271701-tx0lqgff	278	13	)	)	-RRB-
cord-271701-tx0lqgff	278	14	on	on	IN
cord-271701-tx0lqgff	278	15	non	non	JJ
cord-271701-tx0lqgff	278	16	-	-	JJ
cord-271701-tx0lqgff	278	17	structured	structured	JJ
cord-271701-tx0lqgff	278	18	RNA	RNA	NNP
cord-271701-tx0lqgff	278	19	templates	template	NNS
cord-271701-tx0lqgff	278	20	,	,	,
cord-271701-tx0lqgff	278	21	we	-PRON-	PRP
cord-271701-tx0lqgff	278	22	can	can	MD
cord-271701-tx0lqgff	278	23	no	no	RB
cord-271701-tx0lqgff	278	24	longer	longer	RB
cord-271701-tx0lqgff	278	25	exclude	exclude	VB
cord-271701-tx0lqgff	278	26	the	the	DT
cord-271701-tx0lqgff	278	27	possibility	possibility	NN
cord-271701-tx0lqgff	278	28	that	that	IN
cord-271701-tx0lqgff	278	29	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	278	30	+	+	SYM
cord-271701-tx0lqgff	278	31	8	8	CD
cord-271701-tx0lqgff	278	32	)	)	-RRB-
cord-271701-tx0lqgff	278	33	may	may	MD
cord-271701-tx0lqgff	278	34	synthesise	synthesise	VB
cord-271701-tx0lqgff	278	35	substantially	substantially	RB
cord-271701-tx0lqgff	278	36	longer	long	JJR
cord-271701-tx0lqgff	278	37	products	product	NNS
cord-271701-tx0lqgff	278	38	than	than	IN
cord-271701-tx0lqgff	278	39	mere	mere	JJ
cord-271701-tx0lqgff	278	40	oligonucleotide	oligonucleotide	JJ
cord-271701-tx0lqgff	278	41	primers	primer	NNS
cord-271701-tx0lqgff	278	42	in	in	IN
cord-271701-tx0lqgff	278	43	vivo	vivo	NN
cord-271701-tx0lqgff	278	44	,	,	,
cord-271701-tx0lqgff	278	45	possibly	possibly	RB
cord-271701-tx0lqgff	278	46	stimulated	stimulate	VBN
cord-271701-tx0lqgff	278	47	by	by	IN
cord-271701-tx0lqgff	278	48	the	the	DT
cord-271701-tx0lqgff	278	49	presence	presence	NN
cord-271701-tx0lqgff	278	50	of	of	IN
cord-271701-tx0lqgff	278	51	additional	additional	JJ
cord-271701-tx0lqgff	278	52	viral	viral	JJ
cord-271701-tx0lqgff	278	53	protein	protein	NN
cord-271701-tx0lqgff	278	54	factors	factor	NNS
cord-271701-tx0lqgff	278	55	that	that	WDT
cord-271701-tx0lqgff	278	56	could	could	MD
cord-271701-tx0lqgff	278	57	e.g.	e.g.	RB
cord-271701-tx0lqgff	278	58	provide	provide	VB
cord-271701-tx0lqgff	278	59	RNA	RNA	NNP
cord-271701-tx0lqgff	278	60	-	-	HYPH
cord-271701-tx0lqgff	278	61	unwinding	unwind	VBG
cord-271701-tx0lqgff	278	62	activity	activity	NN
cord-271701-tx0lqgff	278	63	.	.	.
cord-271701-tx0lqgff	279	1	Consequently	consequently	RB
cord-271701-tx0lqgff	279	2	,	,	,
cord-271701-tx0lqgff	279	3	it	-PRON-	PRP
cord-271701-tx0lqgff	279	4	is	be	VBZ
cord-271701-tx0lqgff	279	5	now	now	RB
cord-271701-tx0lqgff	279	6	a	a	DT
cord-271701-tx0lqgff	279	7	distinct	distinct	JJ
cord-271701-tx0lqgff	279	8	possibility	possibility	NN
cord-271701-tx0lqgff	279	9	that	that	IN
cord-271701-tx0lqgff	279	10	CoV	CoV	NNP
cord-271701-tx0lqgff	279	11	RNA	RNA	NNP
cord-271701-tx0lqgff	279	12	synthesis	synthesis	NN
cord-271701-tx0lqgff	279	13	involves	involve	VBZ
cord-271701-tx0lqgff	279	14	structurally	structurally	RB
cord-271701-tx0lqgff	279	15	different	different	JJ
cord-271701-tx0lqgff	279	16	and	and	CC
cord-271701-tx0lqgff	279	17	functionally	functionally	RB
cord-271701-tx0lqgff	279	18	separable	separable	JJ
cord-271701-tx0lqgff	279	19	RNA	RNA	NNP
cord-271701-tx0lqgff	279	20	synthesising	synthesise	VBG
cord-271701-tx0lqgff	279	21	complexes	complex	NNS
cord-271701-tx0lqgff	279	22	[	[	-LRB-
cord-271701-tx0lqgff	279	23	e.g.	e.g.	RB
cord-271701-tx0lqgff	279	24	containing	contain	VBG
cord-271701-tx0lqgff	279	25	nsp12	nsp12	CD
cord-271701-tx0lqgff	279	26	or	or	CC
cord-271701-tx0lqgff	279	27	nsp(7	nsp(7	NNS
cord-271701-tx0lqgff	279	28	+	+	SYM
cord-271701-tx0lqgff	279	29	8	8	CD
cord-271701-tx0lqgff	279	30	)	)	-RRB-
cord-271701-tx0lqgff	279	31	]	]	-RRB-
cord-271701-tx0lqgff	279	32	,	,	,
cord-271701-tx0lqgff	279	33	each	each	DT
cord-271701-tx0lqgff	279	34	possessing	possess	VBG
cord-271701-tx0lqgff	279	35	their	-PRON-	PRP$
cord-271701-tx0lqgff	279	36	own	own	JJ
cord-271701-tx0lqgff	279	37	dedicated	dedicated	JJ
cord-271701-tx0lqgff	279	38	RdRp	RdRp	NNP
cord-271701-tx0lqgff	279	39	characteristics	characteristic	NNS
cord-271701-tx0lqgff	279	40	and	and	CC
cord-271701-tx0lqgff	279	41	function	function	VB
cord-271701-tx0lqgff	279	42	in	in	IN
cord-271701-tx0lqgff	279	43	viral	viral	JJ
cord-271701-tx0lqgff	279	44	plus	plus	NN
cord-271701-tx0lqgff	279	45	or	or	CC
cord-271701-tx0lqgff	279	46	minus	minus	CC
cord-271701-tx0lqgff	279	47	strand	strand	NN
cord-271701-tx0lqgff	279	48	RNA	RNA	NNP
cord-271701-tx0lqgff	279	49	synthesis	synthesis	NN
cord-271701-tx0lqgff	279	50	.	.	.
cord-271701-tx0lqgff	280	1	It	-PRON-	PRP
cord-271701-tx0lqgff	280	2	will	will	MD
cord-271701-tx0lqgff	280	3	therefore	therefore	RB
cord-271701-tx0lqgff	280	4	be	be	VB
cord-271701-tx0lqgff	280	5	crucial	crucial	JJ
cord-271701-tx0lqgff	280	6	to	to	TO
cord-271701-tx0lqgff	280	7	study	study	VB
cord-271701-tx0lqgff	280	8	whether	whether	IN
cord-271701-tx0lqgff	280	9	these	these	DT
cord-271701-tx0lqgff	280	10	different	different	JJ
cord-271701-tx0lqgff	280	11	polymerase	polymerase	NN
cord-271701-tx0lqgff	280	12	activities	activity	NNS
cord-271701-tx0lqgff	280	13	are	be	VBP
cord-271701-tx0lqgff	280	14	part	part	NN
cord-271701-tx0lqgff	280	15	of	of	IN
cord-271701-tx0lqgff	280	16	the	the	DT
cord-271701-tx0lqgff	280	17	same	same	JJ
cord-271701-tx0lqgff	280	18	enzyme	enzyme	NN
cord-271701-tx0lqgff	280	19	complex	complex	NN
cord-271701-tx0lqgff	281	1	and	and	CC
cord-271701-tx0lqgff	281	2	,	,	,
cord-271701-tx0lqgff	281	3	if	if	IN
cord-271701-tx0lqgff	281	4	so	so	RB
cord-271701-tx0lqgff	281	5	,	,	,
cord-271701-tx0lqgff	281	6	whether	whether	IN
cord-271701-tx0lqgff	281	7	they	-PRON-	PRP
cord-271701-tx0lqgff	281	8	can	can	MD
cord-271701-tx0lqgff	281	9	influence	influence	VB
cord-271701-tx0lqgff	281	10	each	each	DT
cord-271701-tx0lqgff	281	11	other	other	JJ
cord-271701-tx0lqgff	281	12	's	's	POS
cord-271701-tx0lqgff	281	13	activity	activity	NN
cord-271701-tx0lqgff	281	14	or	or	CC
cord-271701-tx0lqgff	281	15	are	be	VBP
cord-271701-tx0lqgff	281	16	subject	subject	JJ
cord-271701-tx0lqgff	281	17	to	to	IN
cord-271701-tx0lqgff	281	18	additional	additional	JJ
cord-271701-tx0lqgff	281	19	control	control	NN
cord-271701-tx0lqgff	281	20	mechanisms	mechanism	NNS
cord-271701-tx0lqgff	281	21	.	.	.
cord-271701-tx0lqgff	282	1	Host	host	NN
cord-271701-tx0lqgff	282	2	factors	factor	NNS
cord-271701-tx0lqgff	282	3	in	in	IN
cord-271701-tx0lqgff	282	4	positive	positive	JJ
cord-271701-tx0lqgff	282	5	-	-	HYPH
cord-271701-tx0lqgff	282	6	strand	strand	NN
cord-271701-tx0lqgff	282	7	RNA	RNA	NNP
cord-271701-tx0lqgff	282	8	virus	virus	NN
cord-271701-tx0lqgff	282	9	genome	genome	NN
cord-271701-tx0lqgff	282	10	replication	replication	NN
cord-271701-tx0lqgff	282	11	Synthesis	Synthesis	NNP
cord-271701-tx0lqgff	282	12	of	of	IN
cord-271701-tx0lqgff	282	13	subgenomic	subgenomic	NNP
cord-271701-tx0lqgff	282	14	RNAs	rna	NNS
cord-271701-tx0lqgff	282	15	by	by	IN
cord-271701-tx0lqgff	282	16	positive	positive	JJ
cord-271701-tx0lqgff	282	17	-	-	HYPH
cord-271701-tx0lqgff	282	18	strand	strand	NN
cord-271701-tx0lqgff	282	19	RNA	RNA	NNP
cord-271701-tx0lqgff	282	20	viruses	virus	NNS
cord-271701-tx0lqgff	283	1	Two	two	CD
cord-271701-tx0lqgff	283	2	proton	proton	NN
cord-271701-tx0lqgff	283	3	transfers	transfer	NNS
cord-271701-tx0lqgff	283	4	in	in	IN
cord-271701-tx0lqgff	283	5	the	the	DT
cord-271701-tx0lqgff	283	6	transition	transition	NN
cord-271701-tx0lqgff	283	7	state	state	NN
cord-271701-tx0lqgff	283	8	for	for	IN
cord-271701-tx0lqgff	283	9	nucleotidyl	nucleotidyl	NNP
cord-271701-tx0lqgff	283	10	transfer	transfer	NN
cord-271701-tx0lqgff	283	11	catalyzed	catalyze	VBN
cord-271701-tx0lqgff	283	12	by	by	IN
cord-271701-tx0lqgff	283	13	RNA	RNA	NNP
cord-271701-tx0lqgff	283	14	-	-	:
cord-271701-tx0lqgff	283	15	and	and	CC
cord-271701-tx0lqgff	283	16	DNA	dna	NN
cord-271701-tx0lqgff	283	17	-	-	HYPH
cord-271701-tx0lqgff	283	18	dependent	dependent	JJ
cord-271701-tx0lqgff	283	19	RNA	RNA	NNP
cord-271701-tx0lqgff	283	20	and	and	CC
cord-271701-tx0lqgff	283	21	DNA	DNA	NNP
cord-271701-tx0lqgff	283	22	polymerases	polymerase	NNS
cord-271701-tx0lqgff	283	23	Mutation	Mutation	NNP
cord-271701-tx0lqgff	283	24	of	of	IN
cord-271701-tx0lqgff	283	25	the	the	DT
cord-271701-tx0lqgff	283	26	aspartic	aspartic	JJ
cord-271701-tx0lqgff	283	27	acid	acid	NN
cord-271701-tx0lqgff	283	28	residues	residue	NNS
cord-271701-tx0lqgff	283	29	of	of	IN
cord-271701-tx0lqgff	283	30	the	the	DT
cord-271701-tx0lqgff	283	31	GDD	GDD	NNP
cord-271701-tx0lqgff	283	32	sequence	sequence	NN
cord-271701-tx0lqgff	283	33	motif	motif	NN
cord-271701-tx0lqgff	283	34	of	of	IN
cord-271701-tx0lqgff	283	35	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	283	36	RNA	rna	NN
cord-271701-tx0lqgff	283	37	-	-	HYPH
cord-271701-tx0lqgff	283	38	dependent	dependent	JJ
cord-271701-tx0lqgff	283	39	RNA	RNA	NNP
cord-271701-tx0lqgff	283	40	polymerase	polymerase	NN
cord-271701-tx0lqgff	283	41	results	result	NNS
cord-271701-tx0lqgff	283	42	in	in	IN
cord-271701-tx0lqgff	283	43	enzymes	enzyme	NNS
cord-271701-tx0lqgff	283	44	with	with	IN
cord-271701-tx0lqgff	283	45	altered	altered	JJ
cord-271701-tx0lqgff	283	46	metal	metal	NN
cord-271701-tx0lqgff	283	47	ion	ion	NN
cord-271701-tx0lqgff	283	48	requirements	requirement	NNS
cord-271701-tx0lqgff	283	49	for	for	IN
cord-271701-tx0lqgff	283	50	activity	activity	NN
cord-271701-tx0lqgff	283	51	Molecular	molecular	JJ
cord-271701-tx0lqgff	283	52	model	model	NN
cord-271701-tx0lqgff	283	53	of	of	IN
cord-271701-tx0lqgff	283	54	SARS	SARS	NNP
cord-271701-tx0lqgff	283	55	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	283	56	polymerase	polymerase	NN
cord-271701-tx0lqgff	283	57	:	:	:
cord-271701-tx0lqgff	283	58	implications	implication	NNS
cord-271701-tx0lqgff	283	59	for	for	IN
cord-271701-tx0lqgff	283	60	biochemical	biochemical	JJ
cord-271701-tx0lqgff	283	61	functions	function	NNS
cord-271701-tx0lqgff	283	62	and	and	CC
cord-271701-tx0lqgff	283	63	drug	drug	NN
cord-271701-tx0lqgff	283	64	design	design	NN
cord-271701-tx0lqgff	284	1	Coronaviruses	Coronaviruses	NNP
cord-271701-tx0lqgff	284	2	post	post	NN
cord-271701-tx0lqgff	284	3	-	-	JJ
cord-271701-tx0lqgff	284	4	SARS	SARS	NNP
cord-271701-tx0lqgff	285	1	:	:	:
cord-271701-tx0lqgff	285	2	update	update	VB
cord-271701-tx0lqgff	285	3	on	on	IN
cord-271701-tx0lqgff	285	4	replication	replication	NN
cord-271701-tx0lqgff	285	5	and	and	CC
cord-271701-tx0lqgff	285	6	pathogenesis	pathogenesis	NN
cord-271701-tx0lqgff	285	7	Coronaviruses	coronaviruse	NNS
cord-271701-tx0lqgff	285	8	:	:	:
cord-271701-tx0lqgff	286	1	Molecular	molecular	JJ
cord-271701-tx0lqgff	286	2	Biology	biology	NN
cord-271701-tx0lqgff	286	3	and	and	CC
cord-271701-tx0lqgff	286	4	Diseases	disease	NNS
cord-271701-tx0lqgff	287	1	Unique	unique	JJ
cord-271701-tx0lqgff	287	2	and	and	CC
cord-271701-tx0lqgff	287	3	conserved	conserve	VBN
cord-271701-tx0lqgff	287	4	features	feature	NNS
cord-271701-tx0lqgff	287	5	of	of	IN
cord-271701-tx0lqgff	287	6	genome	genome	NN
cord-271701-tx0lqgff	287	7	and	and	CC
cord-271701-tx0lqgff	287	8	proteome	proteome	NN
cord-271701-tx0lqgff	287	9	of	of	IN
cord-271701-tx0lqgff	287	10	SARS	SARS	NNP
cord-271701-tx0lqgff	287	11	-	-	HYPH
cord-271701-tx0lqgff	287	12	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	287	13	,	,	,
cord-271701-tx0lqgff	287	14	an	an	DT
cord-271701-tx0lqgff	287	15	early	early	JJ
cord-271701-tx0lqgff	287	16	split	split	NN
cord-271701-tx0lqgff	287	17	-	-	HYPH
cord-271701-tx0lqgff	287	18	off	off	NN
cord-271701-tx0lqgff	287	19	from	from	IN
cord-271701-tx0lqgff	287	20	the	the	DT
cord-271701-tx0lqgff	287	21	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	287	22	group	group	NN
cord-271701-tx0lqgff	287	23	2	2	CD
cord-271701-tx0lqgff	287	24	lineage	lineage	NN
cord-271701-tx0lqgff	287	25	The	the	DT
cord-271701-tx0lqgff	287	26	RNA	RNA	NNP
cord-271701-tx0lqgff	287	27	polymerase	polymerase	NN
cord-271701-tx0lqgff	287	28	activity	activity	NN
cord-271701-tx0lqgff	287	29	of	of	IN
cord-271701-tx0lqgff	288	1	SARS	SARS	NNP
cord-271701-tx0lqgff	288	2	-	-	HYPH
cord-271701-tx0lqgff	288	3	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	288	4	nsp12	nsp12	CD
cord-271701-tx0lqgff	288	5	is	be	VBZ
cord-271701-tx0lqgff	288	6	primer	primer	JJ
cord-271701-tx0lqgff	288	7	dependent	dependent	JJ
cord-271701-tx0lqgff	288	8	Zn2	zn2	NN
cord-271701-tx0lqgff	288	9	+	+	-LRB-
cord-271701-tx0lqgff	288	10	inhibits	inhibit	VBZ
cord-271701-tx0lqgff	288	11	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	288	12	and	and	CC
cord-271701-tx0lqgff	288	13	arterivirus	arterivirus	NNP
cord-271701-tx0lqgff	288	14	RNA	RNA	NNP
cord-271701-tx0lqgff	288	15	polymerase	polymerase	NN
cord-271701-tx0lqgff	288	16	activity	activity	NN
cord-271701-tx0lqgff	288	17	in	in	IN
cord-271701-tx0lqgff	288	18	vitro	vitro	FW
cord-271701-tx0lqgff	288	19	and	and	CC
cord-271701-tx0lqgff	288	20	zinc	zinc	NN
cord-271701-tx0lqgff	288	21	ionophores	ionophores	NNP
cord-271701-tx0lqgff	288	22	block	block	VBP
cord-271701-tx0lqgff	288	23	the	the	DT
cord-271701-tx0lqgff	288	24	replication	replication	NN
cord-271701-tx0lqgff	288	25	of	of	IN
cord-271701-tx0lqgff	288	26	these	these	DT
cord-271701-tx0lqgff	288	27	viruses	virus	NNS
cord-271701-tx0lqgff	288	28	in	in	IN
cord-271701-tx0lqgff	288	29	cell	cell	NN
cord-271701-tx0lqgff	288	30	culture	culture	NN
cord-271701-tx0lqgff	289	1	A	a	DT
cord-271701-tx0lqgff	289	2	second	second	JJ
cord-271701-tx0lqgff	289	3	,	,	,
cord-271701-tx0lqgff	289	4	non	non	JJ
cord-271701-tx0lqgff	289	5	-	-	JJ
cord-271701-tx0lqgff	289	6	canonical	canonical	JJ
cord-271701-tx0lqgff	289	7	RNA	rna	NN
cord-271701-tx0lqgff	289	8	-	-	HYPH
cord-271701-tx0lqgff	289	9	dependent	dependent	JJ
cord-271701-tx0lqgff	289	10	RNA	RNA	NNP
cord-271701-tx0lqgff	289	11	polymerase	polymerase	NN
cord-271701-tx0lqgff	289	12	in	in	IN
cord-271701-tx0lqgff	289	13	SARS	SARS	NNP
cord-271701-tx0lqgff	289	14	Coronavirus	Coronavirus	NNP
cord-271701-tx0lqgff	289	15	Insights	Insights	NNPS
cord-271701-tx0lqgff	289	16	into	into	IN
cord-271701-tx0lqgff	289	17	SARS	SARS	NNP
cord-271701-tx0lqgff	289	18	-	-	HYPH
cord-271701-tx0lqgff	289	19	CoV	CoV	NNP
cord-271701-tx0lqgff	289	20	transcription	transcription	NN
cord-271701-tx0lqgff	289	21	and	and	CC
cord-271701-tx0lqgff	289	22	replication	replication	NN
cord-271701-tx0lqgff	289	23	from	from	IN
cord-271701-tx0lqgff	289	24	the	the	DT
cord-271701-tx0lqgff	289	25	structure	structure	NN
cord-271701-tx0lqgff	289	26	of	of	IN
cord-271701-tx0lqgff	289	27	the	the	DT
cord-271701-tx0lqgff	289	28	nsp7-nsp8	nsp7-nsp8	NNP
cord-271701-tx0lqgff	289	29	hexadecamer	hexadecamer	NN
cord-271701-tx0lqgff	289	30	Production	Production	NNP
cord-271701-tx0lqgff	289	31	of	of	IN
cord-271701-tx0lqgff	289	32	'	'	``
cord-271701-tx0lqgff	289	33	'	'	``
cord-271701-tx0lqgff	289	34	authentic	authentic	JJ
cord-271701-tx0lqgff	289	35	'	'	''
cord-271701-tx0lqgff	289	36	'	'	''
cord-271701-tx0lqgff	289	37	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	289	38	RNA	rna	NN
cord-271701-tx0lqgff	289	39	-	-	HYPH
cord-271701-tx0lqgff	289	40	dependent	dependent	JJ
cord-271701-tx0lqgff	289	41	RNA	RNA	NNP
cord-271701-tx0lqgff	289	42	polymerase	polymerase	NN
cord-271701-tx0lqgff	289	43	(	(	-LRB-
cord-271701-tx0lqgff	289	44	3D(pol	3d(pol	NN
cord-271701-tx0lqgff	289	45	)	)	-RRB-
cord-271701-tx0lqgff	289	46	)	)	-RRB-
cord-271701-tx0lqgff	289	47	by	by	IN
cord-271701-tx0lqgff	289	48	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	289	49	-	-	HYPH
cord-271701-tx0lqgff	289	50	protease	protease	NN
cord-271701-tx0lqgff	289	51	-	-	HYPH
cord-271701-tx0lqgff	289	52	mediated	mediate	VBN
cord-271701-tx0lqgff	289	53	cleavage	cleavage	NN
cord-271701-tx0lqgff	289	54	in	in	IN
cord-271701-tx0lqgff	289	55	Escherichia	Escherichia	NNP
cord-271701-tx0lqgff	289	56	coli	coli	NNS
cord-271701-tx0lqgff	290	1	A	a	DT
cord-271701-tx0lqgff	290	2	new	new	JJ
cord-271701-tx0lqgff	290	3	lead	lead	NN
cord-271701-tx0lqgff	290	4	for	for	IN
cord-271701-tx0lqgff	290	5	nonpeptidic	nonpeptidic	NN
cord-271701-tx0lqgff	290	6	active	active	JJ
cord-271701-tx0lqgff	290	7	-	-	HYPH
cord-271701-tx0lqgff	290	8	site	site	NN
cord-271701-tx0lqgff	290	9	-	-	HYPH
cord-271701-tx0lqgff	290	10	directed	direct	VBN
cord-271701-tx0lqgff	290	11	inhibitors	inhibitor	NNS
cord-271701-tx0lqgff	290	12	of	of	IN
cord-271701-tx0lqgff	290	13	the	the	DT
cord-271701-tx0lqgff	290	14	severe	severe	JJ
cord-271701-tx0lqgff	290	15	acute	acute	JJ
cord-271701-tx0lqgff	290	16	respiratory	respiratory	JJ
cord-271701-tx0lqgff	290	17	syndrome	syndrome	NN
cord-271701-tx0lqgff	290	18	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	290	19	main	main	JJ
cord-271701-tx0lqgff	290	20	protease	protease	NN
cord-271701-tx0lqgff	290	21	discovered	discover	VBN
cord-271701-tx0lqgff	290	22	by	by	IN
cord-271701-tx0lqgff	290	23	a	a	DT
cord-271701-tx0lqgff	290	24	combination	combination	NN
cord-271701-tx0lqgff	290	25	of	of	IN
cord-271701-tx0lqgff	290	26	screening	screening	NN
cord-271701-tx0lqgff	290	27	and	and	CC
cord-271701-tx0lqgff	290	28	docking	docking	NN
cord-271701-tx0lqgff	290	29	methods	method	NNS
cord-271701-tx0lqgff	290	30	MUSCLE	muscle	NN
cord-271701-tx0lqgff	290	31	:	:	:
cord-271701-tx0lqgff	291	1	multiple	multiple	JJ
cord-271701-tx0lqgff	291	2	sequence	sequence	NN
cord-271701-tx0lqgff	291	3	alignment	alignment	NN
cord-271701-tx0lqgff	291	4	with	with	IN
cord-271701-tx0lqgff	291	5	high	high	JJ
cord-271701-tx0lqgff	291	6	accuracy	accuracy	NN
cord-271701-tx0lqgff	291	7	and	and	CC
cord-271701-tx0lqgff	291	8	high	high	JJ
cord-271701-tx0lqgff	291	9	throughput	throughput	NN
cord-271701-tx0lqgff	291	10	Genome	genome	NN
cord-271701-tx0lqgff	291	11	-	-	HYPH
cord-271701-tx0lqgff	291	12	wide	wide	JJ
cord-271701-tx0lqgff	291	13	analysis	analysis	NN
cord-271701-tx0lqgff	291	14	of	of	IN
cord-271701-tx0lqgff	291	15	protein	protein	NN
cord-271701-tx0lqgff	291	16	-	-	HYPH
cord-271701-tx0lqgff	291	17	protein	protein	NN
cord-271701-tx0lqgff	291	18	interactions	interaction	NNS
cord-271701-tx0lqgff	291	19	and	and	CC
cord-271701-tx0lqgff	291	20	involvement	involvement	NN
cord-271701-tx0lqgff	291	21	of	of	IN
cord-271701-tx0lqgff	291	22	viral	viral	JJ
cord-271701-tx0lqgff	291	23	proteins	protein	NNS
cord-271701-tx0lqgff	291	24	in	in	IN
cord-271701-tx0lqgff	291	25	SARS	SARS	NNP
cord-271701-tx0lqgff	291	26	-	-	HYPH
cord-271701-tx0lqgff	291	27	CoV	CoV	NNP
cord-271701-tx0lqgff	291	28	replication	replication	NN
cord-271701-tx0lqgff	291	29	Structural	structural	JJ
cord-271701-tx0lqgff	291	30	basis	basis	NN
cord-271701-tx0lqgff	291	31	for	for	IN
cord-271701-tx0lqgff	291	32	proteolysis	proteolysis	NN
cord-271701-tx0lqgff	291	33	-	-	HYPH
cord-271701-tx0lqgff	291	34	dependent	dependent	JJ
cord-271701-tx0lqgff	291	35	activation	activation	NN
cord-271701-tx0lqgff	291	36	of	of	IN
cord-271701-tx0lqgff	291	37	the	the	DT
cord-271701-tx0lqgff	291	38	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	291	39	RNA	rna	NN
cord-271701-tx0lqgff	291	40	-	-	HYPH
cord-271701-tx0lqgff	291	41	dependent	dependent	JJ
cord-271701-tx0lqgff	291	42	RNA	RNA	NNP
cord-271701-tx0lqgff	291	43	polymerase	polymerase	NN
cord-271701-tx0lqgff	291	44	Virus	virus	NN
cord-271701-tx0lqgff	291	45	-	-	HYPH
cord-271701-tx0lqgff	291	46	encoded	encode	VBN
cord-271701-tx0lqgff	291	47	proteinases	proteinase	NNS
cord-271701-tx0lqgff	291	48	and	and	CC
cord-271701-tx0lqgff	291	49	proteolytic	proteolytic	JJ
cord-271701-tx0lqgff	291	50	processing	processing	NN
cord-271701-tx0lqgff	291	51	in	in	IN
cord-271701-tx0lqgff	291	52	the	the	DT
cord-271701-tx0lqgff	291	53	Nidovirales	Nidovirales	NNP
cord-271701-tx0lqgff	291	54	Poliovirus	Poliovirus	NNP
cord-271701-tx0lqgff	291	55	RNA	RNA	NNP
cord-271701-tx0lqgff	291	56	-	-	HYPH
cord-271701-tx0lqgff	291	57	dependent	dependent	JJ
cord-271701-tx0lqgff	291	58	RNA	RNA	NNP
cord-271701-tx0lqgff	291	59	Polymerase	Polymerase	NNP
cord-271701-tx0lqgff	291	60	(	(	-LRB-
cord-271701-tx0lqgff	291	61	3Dpol	3Dpol	NNP
cord-271701-tx0lqgff	291	62	)	)	-RRB-
cord-271701-tx0lqgff	291	63	is	be	VBZ
cord-271701-tx0lqgff	291	64	sufficient	sufficient	JJ
cord-271701-tx0lqgff	291	65	for	for	IN
cord-271701-tx0lqgff	291	66	template	template	NN
cord-271701-tx0lqgff	291	67	switching	switch	VBG
cord-271701-tx0lqgff	291	68	in	in	IN
cord-271701-tx0lqgff	292	1	vitro	vitro	NNP
cord-271701-tx0lqgff	292	2	Coronavirus	Coronavirus	NNP
cord-271701-tx0lqgff	292	3	genome	genome	NN
cord-271701-tx0lqgff	292	4	:	:	:
cord-271701-tx0lqgff	292	5	prediction	prediction	NN
cord-271701-tx0lqgff	292	6	of	of	IN
cord-271701-tx0lqgff	292	7	putative	putative	JJ
cord-271701-tx0lqgff	292	8	functional	functional	JJ
cord-271701-tx0lqgff	292	9	domain	domain	NN
cord-271701-tx0lqgff	292	10	in	in	IN
cord-271701-tx0lqgff	292	11	the	the	DT
cord-271701-tx0lqgff	292	12	non	non	JJ
cord-271701-tx0lqgff	292	13	-	-	JJ
cord-271701-tx0lqgff	292	14	structural	structural	JJ
cord-271701-tx0lqgff	292	15	polyprotein	polyprotein	NN
cord-271701-tx0lqgff	292	16	by	by	IN
cord-271701-tx0lqgff	293	1	comparitive	comparitive	JJ
cord-271701-tx0lqgff	293	2	amino	amino	NN
cord-271701-tx0lqgff	293	3	acid	acid	NN
cord-271701-tx0lqgff	293	4	sequence	sequence	NN
cord-271701-tx0lqgff	293	5	analysis	analysis	NN
cord-271701-tx0lqgff	293	6	Origin	origin	NN
cord-271701-tx0lqgff	293	7	and	and	CC
cord-271701-tx0lqgff	293	8	evolution	evolution	NN
cord-271701-tx0lqgff	293	9	of	of	IN
cord-271701-tx0lqgff	293	10	the	the	DT
cord-271701-tx0lqgff	293	11	archeo	archeo	NN
cord-271701-tx0lqgff	293	12	-	-	HYPH
cord-271701-tx0lqgff	293	13	eukaryotic	eukaryotic	JJ
cord-271701-tx0lqgff	293	14	primase	primase	NN
cord-271701-tx0lqgff	293	15	superfamily	superfamily	NN
cord-271701-tx0lqgff	293	16	and	and	CC
cord-271701-tx0lqgff	293	17	related	relate	VBN
cord-271701-tx0lqgff	293	18	palm	palm	NN
cord-271701-tx0lqgff	293	19	-	-	HYPH
cord-271701-tx0lqgff	293	20	domain	domain	NN
cord-271701-tx0lqgff	293	21	proteins	protein	NNS
cord-271701-tx0lqgff	293	22	:	:	:
cord-271701-tx0lqgff	294	1	structural	structural	JJ
cord-271701-tx0lqgff	294	2	insights	insight	NNS
cord-271701-tx0lqgff	294	3	and	and	CC
cord-271701-tx0lqgff	294	4	new	new	JJ
cord-271701-tx0lqgff	294	5	members	member	NNS
cord-271701-tx0lqgff	294	6	Fidelity	Fidelity	NNP
cord-271701-tx0lqgff	294	7	of	of	IN
cord-271701-tx0lqgff	294	8	DNA	dna	NN
cord-271701-tx0lqgff	294	9	synthesis	synthesis	NN
cord-271701-tx0lqgff	294	10	catalyzed	catalyze	VBN
cord-271701-tx0lqgff	294	11	by	by	IN
cord-271701-tx0lqgff	294	12	human	human	JJ
cord-271701-tx0lqgff	294	13	DNA	dna	NN
cord-271701-tx0lqgff	294	14	polymerase	polymerase	NN
cord-271701-tx0lqgff	294	15	alpha	alpha	NN
cord-271701-tx0lqgff	294	16	and	and	CC
cord-271701-tx0lqgff	294	17	HIV-1	HIV-1	NNP
cord-271701-tx0lqgff	294	18	reverse	reverse	VBP
cord-271701-tx0lqgff	294	19	transcriptase	transcriptase	NN
cord-271701-tx0lqgff	294	20	:	:	:
cord-271701-tx0lqgff	294	21	effect	effect	NN
cord-271701-tx0lqgff	294	22	of	of	IN
cord-271701-tx0lqgff	294	23	reaction	reaction	NN
cord-271701-tx0lqgff	295	1	pH	pH	NNP
cord-271701-tx0lqgff	296	1	The	the	DT
cord-271701-tx0lqgff	296	2	broad	broad	JJ
cord-271701-tx0lqgff	296	3	-	-	HYPH
cord-271701-tx0lqgff	296	4	spectrum	spectrum	NN
cord-271701-tx0lqgff	296	5	antiviral	antiviral	JJ
cord-271701-tx0lqgff	296	6	ribonucleoside	ribonucleoside	NN
cord-271701-tx0lqgff	296	7	ribavirin	ribavirin	RB
cord-271701-tx0lqgff	296	8	is	be	VBZ
cord-271701-tx0lqgff	296	9	an	an	DT
cord-271701-tx0lqgff	296	10	RNA	RNA	NNP
cord-271701-tx0lqgff	296	11	virus	virus	NN
cord-271701-tx0lqgff	296	12	mutagen	mutagen	NN
cord-271701-tx0lqgff	296	13	Mechanisms	Mechanisms	NNPS
cord-271701-tx0lqgff	296	14	of	of	IN
cord-271701-tx0lqgff	296	15	action	action	NN
cord-271701-tx0lqgff	296	16	of	of	IN
cord-271701-tx0lqgff	296	17	ribavirin	ribavirin	NN
cord-271701-tx0lqgff	296	18	against	against	IN
cord-271701-tx0lqgff	296	19	distinct	distinct	JJ
cord-271701-tx0lqgff	296	20	viruses	virus	NNS
cord-271701-tx0lqgff	296	21	Inhibitory	inhibitory	JJ
cord-271701-tx0lqgff	296	22	effect	effect	NN
cord-271701-tx0lqgff	296	23	of	of	IN
cord-271701-tx0lqgff	296	24	mizoribine	mizoribine	NN
cord-271701-tx0lqgff	296	25	and	and	CC
cord-271701-tx0lqgff	296	26	ribavirin	ribavirin	NN
cord-271701-tx0lqgff	296	27	on	on	IN
cord-271701-tx0lqgff	296	28	the	the	DT
cord-271701-tx0lqgff	296	29	replication	replication	NN
cord-271701-tx0lqgff	296	30	of	of	IN
cord-271701-tx0lqgff	296	31	severe	severe	JJ
cord-271701-tx0lqgff	296	32	acute	acute	JJ
cord-271701-tx0lqgff	296	33	respiratory	respiratory	JJ
cord-271701-tx0lqgff	296	34	syndrome	syndrome	NN
cord-271701-tx0lqgff	296	35	(	(	-LRB-
cord-271701-tx0lqgff	296	36	SARS)-associated	sars)-associated	CD
cord-271701-tx0lqgff	296	37	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	296	38	Ribavirin	ribavirin	NN
cord-271701-tx0lqgff	296	39	and	and	CC
cord-271701-tx0lqgff	296	40	interferon	interferon	NN
cord-271701-tx0lqgff	296	41	-	-	HYPH
cord-271701-tx0lqgff	296	42	beta	beta	NN
cord-271701-tx0lqgff	297	1	synergistically	synergistically	RB
cord-271701-tx0lqgff	297	2	inhibit	inhibit	VB
cord-271701-tx0lqgff	297	3	SARS	SARS	NNP
cord-271701-tx0lqgff	297	4	-	-	HYPH
cord-271701-tx0lqgff	297	5	associated	associate	VBN
cord-271701-tx0lqgff	297	6	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	297	7	replication	replication	NN
cord-271701-tx0lqgff	297	8	in	in	IN
cord-271701-tx0lqgff	297	9	animal	animal	NN
cord-271701-tx0lqgff	297	10	and	and	CC
cord-271701-tx0lqgff	297	11	human	human	JJ
cord-271701-tx0lqgff	297	12	cell	cell	NN
cord-271701-tx0lqgff	297	13	lines	line	NNS
cord-271701-tx0lqgff	298	1	Ovarian	ovarian	JJ
cord-271701-tx0lqgff	298	2	tumor	tumor	NN
cord-271701-tx0lqgff	298	3	domain	domain	NN
cord-271701-tx0lqgff	298	4	-	-	HYPH
cord-271701-tx0lqgff	298	5	containing	contain	VBG
cord-271701-tx0lqgff	298	6	viral	viral	JJ
cord-271701-tx0lqgff	298	7	proteases	protease	NNS
cord-271701-tx0lqgff	298	8	evade	evade	VBP
cord-271701-tx0lqgff	298	9	ubiquitin	ubiquitin	NN
cord-271701-tx0lqgff	298	10	-	-	:
cord-271701-tx0lqgff	298	11	and	and	CC
cord-271701-tx0lqgff	298	12	ISG15-dependent	isg15-dependent	CD
cord-271701-tx0lqgff	298	13	innate	innate	JJ
cord-271701-tx0lqgff	298	14	immune	immune	JJ
cord-271701-tx0lqgff	298	15	responses	response	NNS
cord-271701-tx0lqgff	298	16	Crystal	Crystal	NNP
cord-271701-tx0lqgff	298	17	structure	structure	NN
cord-271701-tx0lqgff	298	18	of	of	IN
cord-271701-tx0lqgff	298	19	poliovirus	poliovirus	NN
cord-271701-tx0lqgff	298	20	3CD	3cd	CD
cord-271701-tx0lqgff	298	21	protein	protein	NN
cord-271701-tx0lqgff	298	22	:	:	:
cord-271701-tx0lqgff	298	23	virally	virally	RB
cord-271701-tx0lqgff	298	24	encoded	encode	VBN
cord-271701-tx0lqgff	298	25	protease	protease	NN
cord-271701-tx0lqgff	298	26	and	and	CC
cord-271701-tx0lqgff	298	27	precursor	precursor	NN
cord-271701-tx0lqgff	298	28	to	to	IN
cord-271701-tx0lqgff	298	29	the	the	DT
cord-271701-tx0lqgff	298	30	RNA	RNA	NNP
cord-271701-tx0lqgff	298	31	-	-	HYPH
cord-271701-tx0lqgff	298	32	dependent	dependent	JJ
cord-271701-tx0lqgff	298	33	RNA	RNA	NNP
cord-271701-tx0lqgff	298	34	polymerase	polymerase	NN
cord-271701-tx0lqgff	298	35	SARS	SARS	NNP
cord-271701-tx0lqgff	298	36	-	-	HYPH
cord-271701-tx0lqgff	298	37	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	298	38	replication	replication	NN
cord-271701-tx0lqgff	298	39	/	/	SYM
cord-271701-tx0lqgff	298	40	transcription	transcription	NN
cord-271701-tx0lqgff	298	41	complexes	complex	NNS
cord-271701-tx0lqgff	298	42	are	be	VBP
cord-271701-tx0lqgff	298	43	membrane	membrane	NN
cord-271701-tx0lqgff	298	44	-	-	HYPH
cord-271701-tx0lqgff	298	45	protected	protect	VBN
cord-271701-tx0lqgff	298	46	and	and	CC
cord-271701-tx0lqgff	298	47	need	need	VB
cord-271701-tx0lqgff	298	48	a	a	DT
cord-271701-tx0lqgff	298	49	host	host	NN
cord-271701-tx0lqgff	298	50	factor	factor	NN
cord-271701-tx0lqgff	298	51	for	for	IN
cord-271701-tx0lqgff	298	52	activity	activity	NN
cord-271701-tx0lqgff	298	53	in	in	IN
cord-271701-tx0lqgff	298	54	vitro	vitro	FW
cord-271701-tx0lqgff	299	1	The	the	DT
cord-271701-tx0lqgff	299	2	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	299	3	replicase	replicase	NN
cord-271701-tx0lqgff	299	4	:	:	:
cord-271701-tx0lqgff	299	5	insights	insight	NNS
cord-271701-tx0lqgff	299	6	into	into	IN
cord-271701-tx0lqgff	299	7	a	a	DT
cord-271701-tx0lqgff	299	8	sophisticated	sophisticated	JJ
cord-271701-tx0lqgff	299	9	enzyme	enzyme	NN
cord-271701-tx0lqgff	299	10	machinery	machinery	NN
cord-271701-tx0lqgff	299	11	Nidovirus	Nidovirus	NNP
cord-271701-tx0lqgff	299	12	transcription	transcription	NN
cord-271701-tx0lqgff	299	13	:	:	:
cord-271701-tx0lqgff	299	14	how	how	WRB
cord-271701-tx0lqgff	299	15	to	to	TO
cord-271701-tx0lqgff	299	16	make	make	VB
cord-271701-tx0lqgff	299	17	sense	sense	NN
cord-271701-tx0lqgff	299	18	The	the	DT
cord-271701-tx0lqgff	299	19	SARS	SARS	NNP
cord-271701-tx0lqgff	299	20	-	-	HYPH
cord-271701-tx0lqgff	299	21	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	299	22	PLnc	plnc	CD
cord-271701-tx0lqgff	299	23	domain	domain	NN
cord-271701-tx0lqgff	299	24	of	of	IN
cord-271701-tx0lqgff	299	25	nsp3	nsp3	NNS
cord-271701-tx0lqgff	299	26	as	as	IN
cord-271701-tx0lqgff	299	27	a	a	DT
cord-271701-tx0lqgff	299	28	replication	replication	NN
cord-271701-tx0lqgff	299	29	/	/	SYM
cord-271701-tx0lqgff	299	30	transcription	transcription	NN
cord-271701-tx0lqgff	299	31	scaffolding	scaffolding	NN
cord-271701-tx0lqgff	299	32	protein	protein	NN
cord-271701-tx0lqgff	299	33	A	a	DT
cord-271701-tx0lqgff	299	34	noncovalent	noncovalent	JJ
cord-271701-tx0lqgff	299	35	class	class	NN
cord-271701-tx0lqgff	299	36	of	of	IN
cord-271701-tx0lqgff	299	37	papain	papain	NN
cord-271701-tx0lqgff	299	38	-	-	HYPH
cord-271701-tx0lqgff	299	39	like	like	UH
cord-271701-tx0lqgff	299	40	protease/	protease/	NNS
cord-271701-tx0lqgff	299	41	deubiquitinase	deubiquitinase	NN
cord-271701-tx0lqgff	299	42	inhibitors	inhibitor	NNS
cord-271701-tx0lqgff	299	43	blocks	block	VBZ
cord-271701-tx0lqgff	299	44	SARS	SARS	NNP
cord-271701-tx0lqgff	299	45	virus	virus	NN
cord-271701-tx0lqgff	299	46	replication	replication	NN
cord-271701-tx0lqgff	299	47	Multiple	multiple	JJ
cord-271701-tx0lqgff	299	48	enzymatic	enzymatic	JJ
cord-271701-tx0lqgff	299	49	activities	activity	NNS
cord-271701-tx0lqgff	299	50	associated	associate	VBN
cord-271701-tx0lqgff	299	51	with	with	IN
cord-271701-tx0lqgff	299	52	severe	severe	JJ
cord-271701-tx0lqgff	299	53	acute	acute	JJ
cord-271701-tx0lqgff	299	54	respiratory	respiratory	JJ
cord-271701-tx0lqgff	299	55	syndrome	syndrome	NN
cord-271701-tx0lqgff	299	56	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	299	57	helicase	helicase	NN
cord-271701-tx0lqgff	300	1	In	in	IN
cord-271701-tx0lqgff	300	2	vitro	vitro	FW
cord-271701-tx0lqgff	300	3	reconstitution	reconstitution	NN
cord-271701-tx0lqgff	300	4	of	of	IN
cord-271701-tx0lqgff	300	5	SARS	SARS	NNP
cord-271701-tx0lqgff	300	6	-	-	HYPH
cord-271701-tx0lqgff	300	7	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	300	8	mRNA	mrna	NN
cord-271701-tx0lqgff	300	9	cap	cap	NN
cord-271701-tx0lqgff	300	10	methylation	methylation	NN
cord-271701-tx0lqgff	300	11	Processing	processing	NN
cord-271701-tx0lqgff	300	12	of	of	IN
cord-271701-tx0lqgff	300	13	open	open	JJ
cord-271701-tx0lqgff	300	14	reading	reading	NN
cord-271701-tx0lqgff	300	15	frame	frame	NN
cord-271701-tx0lqgff	300	16	1a	1a	CD
cord-271701-tx0lqgff	300	17	replicase	replicase	NNP
cord-271701-tx0lqgff	300	18	proteins	protein	NNS
cord-271701-tx0lqgff	300	19	nsp7	nsp7	PRP
cord-271701-tx0lqgff	300	20	to	to	IN
cord-271701-tx0lqgff	300	21	nsp10	nsp10	PRP
cord-271701-tx0lqgff	300	22	in	in	IN
cord-271701-tx0lqgff	300	23	murine	murine	JJ
cord-271701-tx0lqgff	300	24	hepatitis	hepatitis	NN
cord-271701-tx0lqgff	300	25	virus	virus	NN
cord-271701-tx0lqgff	300	26	strain	strain	NN
cord-271701-tx0lqgff	300	27	A59	A59	NNP
cord-271701-tx0lqgff	300	28	replication	replication	NN
cord-271701-tx0lqgff	301	1	Genetic	genetic	JJ
cord-271701-tx0lqgff	301	2	interactions	interaction	NNS
cord-271701-tx0lqgff	301	3	between	between	IN
cord-271701-tx0lqgff	301	4	an	an	DT
cord-271701-tx0lqgff	301	5	essential	essential	JJ
cord-271701-tx0lqgff	301	6	3	3	CD
cord-271701-tx0lqgff	301	7	0	0	CD
cord-271701-tx0lqgff	301	8	cis	cis	NNP
cord-271701-tx0lqgff	301	9	-	-	HYPH
cord-271701-tx0lqgff	301	10	acting	act	VBG
cord-271701-tx0lqgff	301	11	RNA	RNA	NNP
cord-271701-tx0lqgff	301	12	pseudoknot	pseudoknot	NNP
cord-271701-tx0lqgff	301	13	,	,	,
cord-271701-tx0lqgff	301	14	replicase	replicase	NNP
cord-271701-tx0lqgff	301	15	gene	gene	NN
cord-271701-tx0lqgff	301	16	products	product	NNS
cord-271701-tx0lqgff	301	17	,	,	,
cord-271701-tx0lqgff	301	18	and	and	CC
cord-271701-tx0lqgff	301	19	the	the	DT
cord-271701-tx0lqgff	301	20	extreme	extreme	JJ
cord-271701-tx0lqgff	301	21	3	3	CD
cord-271701-tx0lqgff	301	22	0	0	CD
cord-271701-tx0lqgff	301	23	end	end	NN
cord-271701-tx0lqgff	301	24	of	of	IN
cord-271701-tx0lqgff	301	25	the	the	DT
cord-271701-tx0lqgff	301	26	mouse	mouse	NN
cord-271701-tx0lqgff	301	27	coronavirus	coronavirus	NN
cord-271701-tx0lqgff	301	28	genome	genome	NN
cord-271701-tx0lqgff	302	1	The	the	DT
cord-271701-tx0lqgff	302	2	authors	author	NNS
cord-271701-tx0lqgff	302	3	thank	thank	VBP
cord-271701-tx0lqgff	302	4	Dr	Dr	NNP
cord-271701-tx0lqgff	302	5	Danny	Danny	NNP
cord-271701-tx0lqgff	302	6	Nedialkova	Nedialkova	NNP
cord-271701-tx0lqgff	302	7	,	,	,
cord-271701-tx0lqgff	302	8	Lorenzo	Lorenzo	NNP
cord-271701-tx0lqgff	302	9	Subissi	Subissi	NNP
cord-271701-tx0lqgff	302	10	,	,	,
cord-271701-tx0lqgff	302	11	Dr	Dr	NNP
cord-271701-tx0lqgff	302	12	Isabelle	Isabelle	NNP
cord-271701-tx0lqgff	302	13	Imbert	Imbert	NNP
cord-271701-tx0lqgff	302	14	,	,	,
cord-271701-tx0lqgff	302	15	Dr	Dr	NNP
cord-271701-tx0lqgff	302	16	Bruno	Bruno	NNP
cord-271701-tx0lqgff	302	17	Canard	Canard	NNP
cord-271701-tx0lqgff	302	18	and	and	CC
cord-271701-tx0lqgff	302	19	Dr	Dr	NNP
cord-271701-tx0lqgff	302	20	Alexander	Alexander	NNP
cord-271701-tx0lqgff	302	21	Gorbalenya	Gorbalenya	NNP
cord-271701-tx0lqgff	302	22	for	for	IN
cord-271701-tx0lqgff	302	23	stimulating	stimulate	VBG
cord-271701-tx0lqgff	302	24	discussions	discussion	NNS
cord-271701-tx0lqgff	302	25	;	;	:
cord-271701-tx0lqgff	302	26	Linda	Linda	NNP
cord-271701-tx0lqgff	302	27	Boomaars	Boomaars	NNP
cord-271701-tx0lqgff	302	28	-	-	HYPH
cord-271701-tx0lqgff	302	29	van	van	NNP
cord-271701-tx0lqgff	302	30	der	der	NNP
cord-271701-tx0lqgff	302	31	Zanden	Zanden	NNP
cord-271701-tx0lqgff	302	32	and	and	CC
cord-271701-tx0lqgff	302	33	Dr	Dr	NNP
cord-271701-tx0lqgff	302	34	Clara	Clara	NNP
cord-271701-tx0lqgff	302	35	Posthuma	Posthuma	NNP
cord-271701-tx0lqgff	302	36	for	for	IN
cord-271701-tx0lqgff	302	37	assistance	assistance	NN
cord-271701-tx0lqgff	302	38	with	with	IN
cord-271701-tx0lqgff	302	39	nsp5	nsp5	NN
cord-271701-tx0lqgff	302	40	purification	purification	NN
cord-271701-tx0lqgff	302	41	;	;	:
cord-271701-tx0lqgff	302	42	Puck	Puck	NNP
cord-271701-tx0lqgff	302	43	van	van	NNP
cord-271701-tx0lqgff	302	44	Kasteren	Kasteren	NNP
cord-271701-tx0lqgff	302	45	and	and	CC
cord-271701-tx0lqgff	302	46	Marjolein	Marjolein	NNP
cord-271701-tx0lqgff	302	47	Kikkert	Kikkert	NNP
cord-271701-tx0lqgff	302	48	for	for	IN
cord-271701-tx0lqgff	302	49	providing	provide	VBG
cord-271701-tx0lqgff	302	50	the	the	DT
cord-271701-tx0lqgff	302	51	EAV	EAV	NNP
cord-271701-tx0lqgff	302	52	nsp2	nsp2	NNP
cord-271701-tx0lqgff	302	53	protease	protease	NN
cord-271701-tx0lqgff	302	54	;	;	:
cord-271701-tx0lqgff	302	55	and	and	CC
cord-271701-tx0lqgff	302	56	Jos	Jos	NNP
cord-271701-tx0lqgff	302	57	van	van	NNP
cord-271701-tx0lqgff	302	58	Vugt	Vugt	NNP
cord-271701-tx0lqgff	302	59	for	for	IN
cord-271701-tx0lqgff	302	60	his	-PRON-	PRP$
cord-271701-tx0lqgff	302	61	initial	initial	JJ
cord-271701-tx0lqgff	302	62	work	work	NN
cord-271701-tx0lqgff	302	63	on	on	IN
cord-271701-tx0lqgff	302	64	nsp8	nsp8	NN
cord-271701-tx0lqgff	302	65	in	in	IN
cord-271701-tx0lqgff	302	66	our	-PRON-	PRP$
cord-271701-tx0lqgff	302	67	lab	lab	NN
cord-271701-tx0lqgff	302	68	.	.	.
cord-271701-tx0lqgff	302	69	 	 	_SP
cord-271701-tx0lqgff	303	1	Supplementary	Supplementary	NNP
cord-271701-tx0lqgff	303	2	Data	Data	NNP
cord-271701-tx0lqgff	303	3	are	be	VBP
cord-271701-tx0lqgff	303	4	available	available	JJ
cord-271701-tx0lqgff	303	5	at	at	IN
cord-271701-tx0lqgff	303	6	NAR	NAR	NNP
cord-271701-tx0lqgff	303	7	Online	Online	NNP
cord-271701-tx0lqgff	303	8	.	.	.
cord-271701-tx0lqgff	304	1	Conflict	conflict	NN
cord-271701-tx0lqgff	304	2	of	of	IN
cord-271701-tx0lqgff	304	3	interest	interest	NN
cord-271701-tx0lqgff	304	4	statement	statement	NN
cord-271701-tx0lqgff	304	5	.	.	.
cord-271701-tx0lqgff	305	1	None	none	NN
cord-271701-tx0lqgff	305	2	declared	declare	VBD
cord-271701-tx0lqgff	305	3	.	.	.