id sid tid token lemma pos cord-312431-de7zhswl 1 1 key key NN cord-312431-de7zhswl 1 2 : : : cord-312431-de7zhswl 2 1 cord-312431-de7zhswl cord-312431-de7zhswl NNS cord-312431-de7zhswl 3 1 authors author NNS cord-312431-de7zhswl 3 2 : : : cord-312431-de7zhswl 3 3 Ganesh Ganesh NNP cord-312431-de7zhswl 3 4 , , , cord-312431-de7zhswl 3 5 Atheesha Atheesha NNP cord-312431-de7zhswl 3 6 ; ; : cord-312431-de7zhswl 3 7 Lin Lin NNP cord-312431-de7zhswl 3 8 , , , cord-312431-de7zhswl 3 9 Johnson Johnson NNP cord-312431-de7zhswl 3 10 ; ; : cord-312431-de7zhswl 3 11 Singh Singh NNP cord-312431-de7zhswl 3 12 , , , cord-312431-de7zhswl 3 13 Moganavelli Moganavelli NNP cord-312431-de7zhswl 3 14 title title NN cord-312431-de7zhswl 3 15 : : : cord-312431-de7zhswl 3 16 Detecting detect VBG cord-312431-de7zhswl 3 17 Virus‐Like Virus‐Like NNP cord-312431-de7zhswl 3 18 Particles particle NNS cord-312431-de7zhswl 3 19 from from IN cord-312431-de7zhswl 3 20 the the DT cord-312431-de7zhswl 3 21 Umgeni Umgeni NNP cord-312431-de7zhswl 3 22 River River NNP cord-312431-de7zhswl 3 23 , , , cord-312431-de7zhswl 3 24 South South NNP cord-312431-de7zhswl 3 25 Africa Africa NNP cord-312431-de7zhswl 3 26 date date NN cord-312431-de7zhswl 3 27 : : : cord-312431-de7zhswl 3 28 2013 2013 CD cord-312431-de7zhswl 3 29 - - SYM cord-312431-de7zhswl 3 30 08 08 CD cord-312431-de7zhswl 3 31 - - SYM cord-312431-de7zhswl 3 32 30 30 CD cord-312431-de7zhswl 3 33 journal journal NN cord-312431-de7zhswl 3 34 : : : cord-312431-de7zhswl 3 35 Clean clean JJ cord-312431-de7zhswl 3 36 ( ( -LRB- cord-312431-de7zhswl 3 37 Weinh Weinh NNP cord-312431-de7zhswl 3 38 ) ) -RRB- cord-312431-de7zhswl 3 39 DOI DOI NNP cord-312431-de7zhswl 3 40 : : : cord-312431-de7zhswl 3 41 10.1002 10.1002 CD cord-312431-de7zhswl 3 42 / / SYM cord-312431-de7zhswl 3 43 clen.201200564 clen.201200564 NNP cord-312431-de7zhswl 3 44 sha sha NNP cord-312431-de7zhswl 3 45 : : : cord-312431-de7zhswl 4 1 627a3157889b3523e5ab34c328a82db79cb6fe6d 627a3157889b3523e5ab34c328a82db79cb6fe6d CD cord-312431-de7zhswl 4 2 doc_id doc_id CD cord-312431-de7zhswl 4 3 : : : cord-312431-de7zhswl 4 4 312431 312431 CD cord-312431-de7zhswl 4 5 cord_uid cord_uid NNS cord-312431-de7zhswl 4 6 : : : cord-312431-de7zhswl 4 7 de7zhswl de7zhswl . cord-312431-de7zhswl 5 1 It -PRON- PRP cord-312431-de7zhswl 5 2 is be VBZ cord-312431-de7zhswl 5 3 important important JJ cord-312431-de7zhswl 5 4 to to TO cord-312431-de7zhswl 5 5 consider consider VB cord-312431-de7zhswl 5 6 viruses virus NNS cord-312431-de7zhswl 5 7 in in IN cord-312431-de7zhswl 5 8 water water NN cord-312431-de7zhswl 5 9 quality quality NN cord-312431-de7zhswl 5 10 because because IN cord-312431-de7zhswl 5 11 of of IN cord-312431-de7zhswl 5 12 their -PRON- PRP$ cord-312431-de7zhswl 5 13 incidence incidence NN cord-312431-de7zhswl 5 14 as as IN cord-312431-de7zhswl 5 15 causal causal JJ cord-312431-de7zhswl 5 16 agents agent NNS cord-312431-de7zhswl 5 17 for for IN cord-312431-de7zhswl 5 18 diarrhoeal diarrhoeal NN cord-312431-de7zhswl 5 19 disease disease NN cord-312431-de7zhswl 5 20 , , , cord-312431-de7zhswl 5 21 and and CC cord-312431-de7zhswl 5 22 due due IN cord-312431-de7zhswl 5 23 to to IN cord-312431-de7zhswl 5 24 their -PRON- PRP$ cord-312431-de7zhswl 5 25 characteristics characteristic NNS cord-312431-de7zhswl 5 26 , , , cord-312431-de7zhswl 5 27 which which WDT cord-312431-de7zhswl 5 28 allow allow VBP cord-312431-de7zhswl 5 29 them -PRON- PRP cord-312431-de7zhswl 5 30 to to TO cord-312431-de7zhswl 5 31 survive survive VB cord-312431-de7zhswl 5 32 in in IN cord-312431-de7zhswl 5 33 changing change VBG cord-312431-de7zhswl 5 34 environmental environmental JJ cord-312431-de7zhswl 5 35 conditions condition NNS cord-312431-de7zhswl 5 36 indefinitely indefinitely RB cord-312431-de7zhswl 5 37 . . . cord-312431-de7zhswl 6 1 This this DT cord-312431-de7zhswl 6 2 study study NN cord-312431-de7zhswl 6 3 assessed assess VBD cord-312431-de7zhswl 6 4 the the DT cord-312431-de7zhswl 6 5 viral viral JJ cord-312431-de7zhswl 6 6 quality quality NN cord-312431-de7zhswl 6 7 of of IN cord-312431-de7zhswl 6 8 the the DT cord-312431-de7zhswl 6 9 Umgeni Umgeni NNP cord-312431-de7zhswl 6 10 River River NNP cord-312431-de7zhswl 6 11 in in IN cord-312431-de7zhswl 6 12 South South NNP cord-312431-de7zhswl 6 13 Africa Africa NNP cord-312431-de7zhswl 6 14 seasonally seasonally RB cord-312431-de7zhswl 6 15 . . . cord-312431-de7zhswl 7 1 A a DT cord-312431-de7zhswl 7 2 two‐step two‐step JJ cord-312431-de7zhswl 7 3 tangential tangential JJ cord-312431-de7zhswl 7 4 flow flow NN cord-312431-de7zhswl 7 5 filtration filtration NN cord-312431-de7zhswl 7 6 process process NN cord-312431-de7zhswl 7 7 was be VBD cord-312431-de7zhswl 7 8 setup setup NN cord-312431-de7zhswl 7 9 to to TO cord-312431-de7zhswl 7 10 remove remove VB cord-312431-de7zhswl 7 11 the the DT cord-312431-de7zhswl 7 12 bacteria bacteria NNS cord-312431-de7zhswl 7 13 and and CC cord-312431-de7zhswl 7 14 to to TO cord-312431-de7zhswl 7 15 concentrate concentrate VB cord-312431-de7zhswl 7 16 the the DT cord-312431-de7zhswl 7 17 virus virus NN cord-312431-de7zhswl 7 18 populations population NNS cord-312431-de7zhswl 7 19 from from IN cord-312431-de7zhswl 7 20 large large JJ cord-312431-de7zhswl 7 21 volume volume NN cord-312431-de7zhswl 7 22 water water NN cord-312431-de7zhswl 7 23 samples sample NNS cord-312431-de7zhswl 7 24 . . . cord-312431-de7zhswl 8 1 The the DT cord-312431-de7zhswl 8 2 concentrated concentrated JJ cord-312431-de7zhswl 8 3 water water NN cord-312431-de7zhswl 8 4 samples sample NNS cord-312431-de7zhswl 8 5 contained contain VBN cord-312431-de7zhswl 8 6 up up RP cord-312431-de7zhswl 8 7 to to IN cord-312431-de7zhswl 8 8 659 659 CD cord-312431-de7zhswl 8 9 and and CC cord-312431-de7zhswl 8 10 550 550 CD cord-312431-de7zhswl 8 11 pfu pfu NN cord-312431-de7zhswl 8 12 / / SYM cord-312431-de7zhswl 8 13 mL ml NN cord-312431-de7zhswl 8 14 of of IN cord-312431-de7zhswl 8 15 somatic somatic JJ cord-312431-de7zhswl 8 16 and and CC cord-312431-de7zhswl 8 17 F‐RNA F‐RNA NNP cord-312431-de7zhswl 8 18 coliphages coliphage NNS cord-312431-de7zhswl 8 19 , , , cord-312431-de7zhswl 8 20 respectively respectively RB cord-312431-de7zhswl 8 21 . . . cord-312431-de7zhswl 9 1 Several several JJ cord-312431-de7zhswl 9 2 virus virus NN cord-312431-de7zhswl 9 3 families family NNS cord-312431-de7zhswl 9 4 including include VBG cord-312431-de7zhswl 9 5 Adenoviridae Adenoviridae NNP cord-312431-de7zhswl 9 6 , , , cord-312431-de7zhswl 9 7 Herpesviridae Herpesviridae NNP cord-312431-de7zhswl 9 8 , , , cord-312431-de7zhswl 9 9 Orthomyxoviridae Orthomyxoviridae NNP cord-312431-de7zhswl 9 10 , , , cord-312431-de7zhswl 9 11 Picornaviridae Picornaviridae NNP cord-312431-de7zhswl 9 12 , , , cord-312431-de7zhswl 9 13 Poxviridae Poxviridae NNP cord-312431-de7zhswl 9 14 and and CC cord-312431-de7zhswl 9 15 Reoviridae Reoviridae NNP cord-312431-de7zhswl 9 16 were be VBD cord-312431-de7zhswl 9 17 found find VBN cord-312431-de7zhswl 9 18 in in IN cord-312431-de7zhswl 9 19 the the DT cord-312431-de7zhswl 9 20 river river NN cord-312431-de7zhswl 9 21 based base VBN cord-312431-de7zhswl 9 22 on on IN cord-312431-de7zhswl 9 23 the the DT cord-312431-de7zhswl 9 24 morphologies morphology NNS cord-312431-de7zhswl 9 25 examined examine VBN cord-312431-de7zhswl 9 26 under under IN cord-312431-de7zhswl 9 27 transmission transmission NN cord-312431-de7zhswl 9 28 electron electron NN cord-312431-de7zhswl 9 29 microscopy microscopy NN cord-312431-de7zhswl 9 30 . . . cord-312431-de7zhswl 10 1 All all DT cord-312431-de7zhswl 10 2 concentrated concentrated JJ cord-312431-de7zhswl 10 3 water water NN cord-312431-de7zhswl 10 4 samples sample NNS cord-312431-de7zhswl 10 5 produced produce VBD cord-312431-de7zhswl 10 6 substantial substantial JJ cord-312431-de7zhswl 10 7 cytopathic cytopathic JJ cord-312431-de7zhswl 10 8 effects effect NNS cord-312431-de7zhswl 10 9 on on IN cord-312431-de7zhswl 10 10 the the DT cord-312431-de7zhswl 10 11 Vero Vero NNP cord-312431-de7zhswl 10 12 , , , cord-312431-de7zhswl 10 13 HEK HEK NNP cord-312431-de7zhswl 10 14 293 293 CD cord-312431-de7zhswl 10 15 , , , cord-312431-de7zhswl 10 16 Hela Hela NNP cord-312431-de7zhswl 10 17 and and CC cord-312431-de7zhswl 10 18 A549 a549 JJ cord-312431-de7zhswl 10 19 cell cell NN cord-312431-de7zhswl 10 20 lines line NNS cord-312431-de7zhswl 10 21 . . . cord-312431-de7zhswl 11 1 These these DT cord-312431-de7zhswl 11 2 results result NNS cord-312431-de7zhswl 11 3 indicate indicate VBP cord-312431-de7zhswl 11 4 the the DT cord-312431-de7zhswl 11 5 potential potential NN cord-312431-de7zhswl 11 6 of of IN cord-312431-de7zhswl 11 7 viruses virus NNS cord-312431-de7zhswl 11 8 in in IN cord-312431-de7zhswl 11 9 the the DT cord-312431-de7zhswl 11 10 water water NN cord-312431-de7zhswl 11 11 samples sample NNS cord-312431-de7zhswl 11 12 especially especially RB cord-312431-de7zhswl 11 13 from from IN cord-312431-de7zhswl 11 14 the the DT cord-312431-de7zhswl 11 15 lower low JJR cord-312431-de7zhswl 11 16 catchment catchment NN cord-312431-de7zhswl 11 17 areas area NNS cord-312431-de7zhswl 11 18 of of IN cord-312431-de7zhswl 11 19 the the DT cord-312431-de7zhswl 11 20 Umgeni Umgeni NNP cord-312431-de7zhswl 11 21 River River NNP cord-312431-de7zhswl 11 22 to to TO cord-312431-de7zhswl 11 23 infect infect VB cord-312431-de7zhswl 11 24 human human JJ cord-312431-de7zhswl 11 25 hosts host NNS cord-312431-de7zhswl 11 26 throughout throughout IN cord-312431-de7zhswl 11 27 the the DT cord-312431-de7zhswl 11 28 year year NN cord-312431-de7zhswl 11 29 . . . cord-312431-de7zhswl 12 1 The the DT cord-312431-de7zhswl 12 2 present present JJ cord-312431-de7zhswl 12 3 study study NN cord-312431-de7zhswl 12 4 highlights highlight VBZ cord-312431-de7zhswl 12 5 the the DT cord-312431-de7zhswl 12 6 importance importance NN cord-312431-de7zhswl 12 7 of of IN cord-312431-de7zhswl 12 8 routine routine JJ cord-312431-de7zhswl 12 9 environmental environmental JJ cord-312431-de7zhswl 12 10 surveillance surveillance NN cord-312431-de7zhswl 12 11 of of IN cord-312431-de7zhswl 12 12 human human JJ cord-312431-de7zhswl 12 13 enteric enteric JJ cord-312431-de7zhswl 12 14 viruses virus NNS cord-312431-de7zhswl 12 15 in in IN cord-312431-de7zhswl 12 16 water water NN cord-312431-de7zhswl 12 17 sources source NNS cord-312431-de7zhswl 12 18 . . . cord-312431-de7zhswl 13 1 This this DT cord-312431-de7zhswl 13 2 can can MD cord-312431-de7zhswl 13 3 contribute contribute VB cord-312431-de7zhswl 13 4 to to IN cord-312431-de7zhswl 13 5 a a DT cord-312431-de7zhswl 13 6 better well JJR cord-312431-de7zhswl 13 7 understanding understanding NN cord-312431-de7zhswl 13 8 of of IN cord-312431-de7zhswl 13 9 the the DT cord-312431-de7zhswl 13 10 actual actual JJ cord-312431-de7zhswl 13 11 burden burden NN cord-312431-de7zhswl 13 12 of of IN cord-312431-de7zhswl 13 13 disease disease NN cord-312431-de7zhswl 13 14 on on IN cord-312431-de7zhswl 13 15 those those DT cord-312431-de7zhswl 13 16 who who WP cord-312431-de7zhswl 13 17 might may MD cord-312431-de7zhswl 13 18 be be VB cord-312431-de7zhswl 13 19 using use VBG cord-312431-de7zhswl 13 20 the the DT cord-312431-de7zhswl 13 21 water water NN cord-312431-de7zhswl 13 22 directly directly RB cord-312431-de7zhswl 13 23 without without IN cord-312431-de7zhswl 13 24 treatment treatment NN cord-312431-de7zhswl 13 25 . . . cord-312431-de7zhswl 14 1 Despite despite IN cord-312431-de7zhswl 14 2 the the DT cord-312431-de7zhswl 14 3 advancement advancement NN cord-312431-de7zhswl 14 4 in in IN cord-312431-de7zhswl 14 5 water water NN cord-312431-de7zhswl 14 6 and and CC cord-312431-de7zhswl 14 7 wastewater wastewater NN cord-312431-de7zhswl 14 8 management management NN cord-312431-de7zhswl 14 9 proficiency proficiency NN cord-312431-de7zhswl 14 10 , , , cord-312431-de7zhswl 14 11 faecal faecal JJ cord-312431-de7zhswl 14 12 pollution pollution NN cord-312431-de7zhswl 14 13 of of IN cord-312431-de7zhswl 14 14 surface surface NN cord-312431-de7zhswl 14 15 waters water NNS cord-312431-de7zhswl 14 16 is be VBZ cord-312431-de7zhswl 14 17 still still RB cord-312431-de7zhswl 14 18 a a DT cord-312431-de7zhswl 14 19 cause cause NN cord-312431-de7zhswl 14 20 of of IN cord-312431-de7zhswl 14 21 concern concern NN cord-312431-de7zhswl 14 22 for for IN cord-312431-de7zhswl 14 23 the the DT cord-312431-de7zhswl 14 24 general general JJ cord-312431-de7zhswl 14 25 public public NN cord-312431-de7zhswl 14 26 and and CC cord-312431-de7zhswl 14 27 can can MD cord-312431-de7zhswl 14 28 lead lead VB cord-312431-de7zhswl 14 29 to to IN cord-312431-de7zhswl 14 30 waterborne waterborne JJ cord-312431-de7zhswl 14 31 illnesses illness NNS cord-312431-de7zhswl 14 32 which which WDT cord-312431-de7zhswl 14 33 have have VBP cord-312431-de7zhswl 14 34 community community NN cord-312431-de7zhswl 14 35 well well RB cord-312431-de7zhswl 14 36 being be VBG cord-312431-de7zhswl 14 37 and and CC cord-312431-de7zhswl 14 38 socio socio JJ cord-312431-de7zhswl 14 39 - - JJ cord-312431-de7zhswl 14 40 economic economic JJ cord-312431-de7zhswl 14 41 repercussions repercussion NNS cord-312431-de7zhswl 14 42 in in IN cord-312431-de7zhswl 14 43 both both CC cord-312431-de7zhswl 14 44 the the DT cord-312431-de7zhswl 14 45 industrialised industrialised JJ cord-312431-de7zhswl 14 46 and and CC cord-312431-de7zhswl 14 47 developing develop VBG cord-312431-de7zhswl 14 48 world world NN cord-312431-de7zhswl 14 49 [ [ -LRB- cord-312431-de7zhswl 14 50 1 1 CD cord-312431-de7zhswl 14 51 , , , cord-312431-de7zhswl 14 52 2 2 CD cord-312431-de7zhswl 14 53 ] ] -RRB- cord-312431-de7zhswl 14 54 . . . cord-312431-de7zhswl 15 1 Waterborne waterborne JJ cord-312431-de7zhswl 15 2 diseases disease NNS cord-312431-de7zhswl 15 3 are be VBP cord-312431-de7zhswl 15 4 frequently frequently RB cord-312431-de7zhswl 15 5 caused cause VBN cord-312431-de7zhswl 15 6 by by IN cord-312431-de7zhswl 15 7 enteric enteric JJ cord-312431-de7zhswl 15 8 pathogens pathogen NNS cord-312431-de7zhswl 15 9 , , , cord-312431-de7zhswl 15 10 which which WDT cord-312431-de7zhswl 15 11 belong belong VBP cord-312431-de7zhswl 15 12 to to IN cord-312431-de7zhswl 15 13 the the DT cord-312431-de7zhswl 15 14 group group NN cord-312431-de7zhswl 15 15 of of IN cord-312431-de7zhswl 15 16 organisms organism NNS cord-312431-de7zhswl 15 17 transmitted transmit VBN cord-312431-de7zhswl 15 18 by by IN cord-312431-de7zhswl 15 19 the the DT cord-312431-de7zhswl 15 20 faecal faecal JJ cord-312431-de7zhswl 15 21 - - HYPH cord-312431-de7zhswl 15 22 oral oral JJ cord-312431-de7zhswl 15 23 route route NN cord-312431-de7zhswl 15 24 [ [ -LRB- cord-312431-de7zhswl 15 25 3 3 CD cord-312431-de7zhswl 15 26 ] ] -RRB- cord-312431-de7zhswl 15 27 . . . cord-312431-de7zhswl 16 1 The the DT cord-312431-de7zhswl 16 2 World World NNP cord-312431-de7zhswl 16 3 Health Health NNP cord-312431-de7zhswl 16 4 Organisation Organisation NNP cord-312431-de7zhswl 16 5 ( ( -LRB- cord-312431-de7zhswl 16 6 WHO who WP cord-312431-de7zhswl 16 7 ) ) -RRB- cord-312431-de7zhswl 16 8 , , , cord-312431-de7zhswl 16 9 states state VBZ cord-312431-de7zhswl 16 10 that that IN cord-312431-de7zhswl 16 11 there there EX cord-312431-de7zhswl 16 12 are be VBP cord-312431-de7zhswl 16 13 $ $ $ cord-312431-de7zhswl 16 14 2.2 2.2 CD cord-312431-de7zhswl 16 15 million million CD cord-312431-de7zhswl 16 16 deaths death NNS cord-312431-de7zhswl 16 17 related relate VBN cord-312431-de7zhswl 16 18 with with IN cord-312431-de7zhswl 16 19 hazardous hazardous JJ cord-312431-de7zhswl 16 20 water water NN cord-312431-de7zhswl 16 21 conditions condition NNS cord-312431-de7zhswl 16 22 , , , cord-312431-de7zhswl 16 23 sanitation sanitation NN cord-312431-de7zhswl 16 24 and and CC cord-312431-de7zhswl 16 25 hygiene hygiene NN cord-312431-de7zhswl 16 26 , , , cord-312431-de7zhswl 16 27 and and CC cord-312431-de7zhswl 16 28 millions million NNS cord-312431-de7zhswl 16 29 more more RBR cord-312431-de7zhswl 16 30 suffer suffer VBP cord-312431-de7zhswl 16 31 numerous numerous JJ cord-312431-de7zhswl 16 32 incidents incident NNS cord-312431-de7zhswl 16 33 of of IN cord-312431-de7zhswl 16 34 non non JJ cord-312431-de7zhswl 16 35 - - JJ cord-312431-de7zhswl 16 36 fatal fatal JJ cord-312431-de7zhswl 16 37 diarrhoea diarrhoea NN cord-312431-de7zhswl 16 38 every every DT cord-312431-de7zhswl 16 39 year year NN cord-312431-de7zhswl 16 40 [ [ -LRB- cord-312431-de7zhswl 16 41 4 4 CD cord-312431-de7zhswl 16 42 ] ] -RRB- cord-312431-de7zhswl 16 43 . . . cord-312431-de7zhswl 17 1 Water water NN cord-312431-de7zhswl 17 2 quality quality NN cord-312431-de7zhswl 17 3 concerns concern NNS cord-312431-de7zhswl 17 4 have have VBP cord-312431-de7zhswl 17 5 increased increase VBN cord-312431-de7zhswl 17 6 in in IN cord-312431-de7zhswl 17 7 recent recent JJ cord-312431-de7zhswl 17 8 years year NNS cord-312431-de7zhswl 17 9 , , , cord-312431-de7zhswl 17 10 in in IN cord-312431-de7zhswl 17 11 part part NN cord-312431-de7zhswl 17 12 due due IN cord-312431-de7zhswl 17 13 to to IN cord-312431-de7zhswl 17 14 recurrent recurrent JJ cord-312431-de7zhswl 17 15 contamination contamination NN cord-312431-de7zhswl 17 16 of of IN cord-312431-de7zhswl 17 17 surface surface NN cord-312431-de7zhswl 17 18 water water NN cord-312431-de7zhswl 17 19 resources resource NNS cord-312431-de7zhswl 17 20 by by IN cord-312431-de7zhswl 17 21 waterborne waterborne JJ cord-312431-de7zhswl 17 22 bacterial bacterial JJ cord-312431-de7zhswl 17 23 , , , cord-312431-de7zhswl 17 24 viral viral JJ cord-312431-de7zhswl 17 25 and and CC cord-312431-de7zhswl 17 26 protozoan protozoan NN cord-312431-de7zhswl 17 27 pathogens pathogen NNS cord-312431-de7zhswl 17 28 [ [ -LRB- cord-312431-de7zhswl 17 29 5 5 CD cord-312431-de7zhswl 17 30 ] ] -RRB- cord-312431-de7zhswl 17 31 . . . cord-312431-de7zhswl 18 1 Human human JJ cord-312431-de7zhswl 18 2 enteric enteric JJ cord-312431-de7zhswl 18 3 viruses virus NNS cord-312431-de7zhswl 18 4 are be VBP cord-312431-de7zhswl 18 5 imperative imperative JJ cord-312431-de7zhswl 18 6 causative causative JJ cord-312431-de7zhswl 18 7 agents agent NNS cord-312431-de7zhswl 18 8 of of IN cord-312431-de7zhswl 18 9 waterborne waterborne JJ cord-312431-de7zhswl 18 10 illness illness NN cord-312431-de7zhswl 18 11 , , , cord-312431-de7zhswl 18 12 however however RB cord-312431-de7zhswl 18 13 , , , cord-312431-de7zhswl 18 14 viral viral JJ cord-312431-de7zhswl 18 15 diseases disease NNS cord-312431-de7zhswl 18 16 are be VBP cord-312431-de7zhswl 18 17 difficult difficult JJ cord-312431-de7zhswl 18 18 to to TO cord-312431-de7zhswl 18 19 identify identify VB cord-312431-de7zhswl 18 20 by by IN cord-312431-de7zhswl 18 21 current current JJ cord-312431-de7zhswl 18 22 diagnostic diagnostic JJ cord-312431-de7zhswl 18 23 techniques technique NNS cord-312431-de7zhswl 18 24 [ [ -LRB- cord-312431-de7zhswl 18 25 6 6 CD cord-312431-de7zhswl 18 26 , , , cord-312431-de7zhswl 18 27 7 7 CD cord-312431-de7zhswl 18 28 ] ] -RRB- cord-312431-de7zhswl 18 29 . . . cord-312431-de7zhswl 19 1 The the DT cord-312431-de7zhswl 19 2 Centres Centres NNPS cord-312431-de7zhswl 19 3 for for IN cord-312431-de7zhswl 19 4 Disease Disease NNP cord-312431-de7zhswl 19 5 Control Control NNP cord-312431-de7zhswl 19 6 and and CC cord-312431-de7zhswl 19 7 Prevention Prevention NNP cord-312431-de7zhswl 19 8 [ [ -LRB- cord-312431-de7zhswl 19 9 8 8 CD cord-312431-de7zhswl 19 10 ] ] -RRB- cord-312431-de7zhswl 19 11 projected project VBN cord-312431-de7zhswl 19 12 that that IN cord-312431-de7zhswl 19 13 viral viral JJ cord-312431-de7zhswl 19 14 infection infection NN cord-312431-de7zhswl 19 15 may may MD cord-312431-de7zhswl 19 16 be be VB cord-312431-de7zhswl 19 17 responsible responsible JJ cord-312431-de7zhswl 19 18 for for IN cord-312431-de7zhswl 19 19 almost almost RB cord-312431-de7zhswl 19 20 half half NN cord-312431-de7zhswl 19 21 of of IN cord-312431-de7zhswl 19 22 all all DT cord-312431-de7zhswl 19 23 acute acute JJ cord-312431-de7zhswl 19 24 gastrointestinal gastrointestinal JJ cord-312431-de7zhswl 19 25 diseases disease NNS cord-312431-de7zhswl 19 26 , , , cord-312431-de7zhswl 19 27 and and CC cord-312431-de7zhswl 19 28 therefore therefore RB cord-312431-de7zhswl 19 29 , , , cord-312431-de7zhswl 19 30 viral viral JJ cord-312431-de7zhswl 19 31 contamination contamination NN cord-312431-de7zhswl 19 32 of of IN cord-312431-de7zhswl 19 33 recreational recreational JJ cord-312431-de7zhswl 19 34 coastal coastal JJ cord-312431-de7zhswl 19 35 water water NN cord-312431-de7zhswl 19 36 is be VBZ cord-312431-de7zhswl 19 37 of of IN cord-312431-de7zhswl 19 38 paramount paramount JJ cord-312431-de7zhswl 19 39 importance importance NN cord-312431-de7zhswl 19 40 and and CC cord-312431-de7zhswl 19 41 is be VBZ cord-312431-de7zhswl 19 42 a a DT cord-312431-de7zhswl 19 43 rising rise VBG cord-312431-de7zhswl 19 44 public public JJ cord-312431-de7zhswl 19 45 health health NN cord-312431-de7zhswl 19 46 concern concern NN cord-312431-de7zhswl 19 47 [ [ -LRB- cord-312431-de7zhswl 19 48 9 9 CD cord-312431-de7zhswl 19 49 ] ] -RRB- cord-312431-de7zhswl 19 50 . . . cord-312431-de7zhswl 20 1 Enteric enteric JJ cord-312431-de7zhswl 20 2 viruses virus NNS cord-312431-de7zhswl 20 3 have have VBP cord-312431-de7zhswl 20 4 a a DT cord-312431-de7zhswl 20 5 relatively relatively RB cord-312431-de7zhswl 20 6 low low JJ cord-312431-de7zhswl 20 7 infectious infectious JJ cord-312431-de7zhswl 20 8 dose dose NN cord-312431-de7zhswl 20 9 and and CC cord-312431-de7zhswl 20 10 can can MD cord-312431-de7zhswl 20 11 be be VB cord-312431-de7zhswl 20 12 responsible responsible JJ cord-312431-de7zhswl 20 13 for for IN cord-312431-de7zhswl 20 14 a a DT cord-312431-de7zhswl 20 15 large large JJ cord-312431-de7zhswl 20 16 range range NN cord-312431-de7zhswl 20 17 of of IN cord-312431-de7zhswl 20 18 human human JJ cord-312431-de7zhswl 20 19 illnesses illness NNS cord-312431-de7zhswl 20 20 such such JJ cord-312431-de7zhswl 20 21 as as IN cord-312431-de7zhswl 20 22 paralysis paralysis NN cord-312431-de7zhswl 20 23 , , , cord-312431-de7zhswl 20 24 meningitis meningitis NN cord-312431-de7zhswl 20 25 , , , cord-312431-de7zhswl 20 26 respiratory respiratory JJ cord-312431-de7zhswl 20 27 diseases disease NNS cord-312431-de7zhswl 20 28 , , , cord-312431-de7zhswl 20 29 myocarditis myocarditis NN cord-312431-de7zhswl 20 30 , , , cord-312431-de7zhswl 20 31 congenital congenital JJ cord-312431-de7zhswl 20 32 abnormalities abnormality NNS cord-312431-de7zhswl 20 33 , , , cord-312431-de7zhswl 20 34 epidemic epidemic NN cord-312431-de7zhswl 20 35 vomiting vomiting NN cord-312431-de7zhswl 20 36 , , , cord-312431-de7zhswl 20 37 diarrhoea diarrhoea NN cord-312431-de7zhswl 20 38 and and CC cord-312431-de7zhswl 20 39 hepatitis hepatitis NN cord-312431-de7zhswl 20 40 [ [ -LRB- cord-312431-de7zhswl 20 41 10 10 CD cord-312431-de7zhswl 20 42 , , , cord-312431-de7zhswl 20 43 11 11 CD cord-312431-de7zhswl 20 44 ] ] -RRB- cord-312431-de7zhswl 20 45 . . . cord-312431-de7zhswl 21 1 Nevertheless nevertheless RB cord-312431-de7zhswl 21 2 , , , cord-312431-de7zhswl 21 3 it -PRON- PRP cord-312431-de7zhswl 21 4 is be VBZ cord-312431-de7zhswl 21 5 now now RB cord-312431-de7zhswl 21 6 accepted accept VBN cord-312431-de7zhswl 21 7 that that IN cord-312431-de7zhswl 21 8 viral viral JJ cord-312431-de7zhswl 21 9 contamination contamination NN cord-312431-de7zhswl 21 10 should should MD cord-312431-de7zhswl 21 11 be be VB cord-312431-de7zhswl 21 12 definitely definitely RB cord-312431-de7zhswl 21 13 taken take VBN cord-312431-de7zhswl 21 14 into into IN cord-312431-de7zhswl 21 15 account account NN cord-312431-de7zhswl 21 16 in in IN cord-312431-de7zhswl 21 17 the the DT cord-312431-de7zhswl 21 18 microbiological microbiological JJ cord-312431-de7zhswl 21 19 analysis analysis NN cord-312431-de7zhswl 21 20 of of IN cord-312431-de7zhswl 21 21 water water NN cord-312431-de7zhswl 21 22 and and CC cord-312431-de7zhswl 21 23 food food NN cord-312431-de7zhswl 21 24 [ [ -LRB- cord-312431-de7zhswl 21 25 12 12 CD cord-312431-de7zhswl 21 26 ] ] -RRB- cord-312431-de7zhswl 21 27 . . . cord-312431-de7zhswl 22 1 Currently currently RB cord-312431-de7zhswl 22 2 microbiological microbiological JJ cord-312431-de7zhswl 22 3 quality quality NN cord-312431-de7zhswl 22 4 standards standard NNS cord-312431-de7zhswl 22 5 for for IN cord-312431-de7zhswl 22 6 water water NN cord-312431-de7zhswl 22 7 include include VBP cord-312431-de7zhswl 22 8 mostly mostly RB cord-312431-de7zhswl 22 9 bacterial bacterial JJ cord-312431-de7zhswl 22 10 faecal faecal JJ cord-312431-de7zhswl 22 11 indicators indicator NNS cord-312431-de7zhswl 22 12 , , , cord-312431-de7zhswl 22 13 which which WDT cord-312431-de7zhswl 22 14 are be VBP cord-312431-de7zhswl 22 15 not not RB cord-312431-de7zhswl 22 16 correlated correlate VBN cord-312431-de7zhswl 22 17 with with IN cord-312431-de7zhswl 22 18 the the DT cord-312431-de7zhswl 22 19 presence presence NN cord-312431-de7zhswl 22 20 of of IN cord-312431-de7zhswl 22 21 waterborne waterborne JJ cord-312431-de7zhswl 22 22 viruses virus NNS cord-312431-de7zhswl 22 23 [ [ -LRB- cord-312431-de7zhswl 22 24 13 13 CD cord-312431-de7zhswl 22 25 ] ] -RRB- cord-312431-de7zhswl 22 26 . . . cord-312431-de7zhswl 23 1 Routine routine JJ cord-312431-de7zhswl 23 2 viral viral JJ cord-312431-de7zhswl 23 3 monitoring monitoring NN cord-312431-de7zhswl 23 4 is be VBZ cord-312431-de7zhswl 23 5 not not RB cord-312431-de7zhswl 23 6 required require VBN cord-312431-de7zhswl 23 7 for for IN cord-312431-de7zhswl 23 8 drinking drinking NN cord-312431-de7zhswl 23 9 or or CC cord-312431-de7zhswl 23 10 recreational recreational JJ cord-312431-de7zhswl 23 11 waters water NNS cord-312431-de7zhswl 23 12 and and CC cord-312431-de7zhswl 23 13 neither neither DT cord-312431-de7zhswl 23 14 is be VBZ cord-312431-de7zhswl 23 15 it -PRON- PRP cord-312431-de7zhswl 23 16 required require VBN cord-312431-de7zhswl 23 17 for for IN cord-312431-de7zhswl 23 18 wastewater wastewater NN cord-312431-de7zhswl 23 19 that that WDT cord-312431-de7zhswl 23 20 is be VBZ cord-312431-de7zhswl 23 21 discharged discharge VBN cord-312431-de7zhswl 23 22 into into IN cord-312431-de7zhswl 23 23 the the DT cord-312431-de7zhswl 23 24 environment environment NN cord-312431-de7zhswl 23 25 . . . cord-312431-de7zhswl 24 1 This this DT cord-312431-de7zhswl 24 2 lack lack NN cord-312431-de7zhswl 24 3 of of IN cord-312431-de7zhswl 24 4 a a DT cord-312431-de7zhswl 24 5 monitoring monitoring NN cord-312431-de7zhswl 24 6 effort effort NN cord-312431-de7zhswl 24 7 is be VBZ cord-312431-de7zhswl 24 8 due due JJ cord-312431-de7zhswl 24 9 largely largely RB cord-312431-de7zhswl 24 10 to to IN cord-312431-de7zhswl 24 11 the the DT cord-312431-de7zhswl 24 12 lack lack NN cord-312431-de7zhswl 24 13 of of IN cord-312431-de7zhswl 24 14 methods method NNS cord-312431-de7zhswl 24 15 that that WDT cord-312431-de7zhswl 24 16 can can MD cord-312431-de7zhswl 24 17 rapidly rapidly RB cord-312431-de7zhswl 24 18 and and CC cord-312431-de7zhswl 24 19 sensitively sensitively RB cord-312431-de7zhswl 24 20 detect detect VB cord-312431-de7zhswl 24 21 infectious infectious JJ cord-312431-de7zhswl 24 22 viruses virus NNS cord-312431-de7zhswl 24 23 in in IN cord-312431-de7zhswl 24 24 environmental environmental JJ cord-312431-de7zhswl 24 25 samples sample NNS cord-312431-de7zhswl 24 26 [ [ -LRB- cord-312431-de7zhswl 24 27 14 14 CD cord-312431-de7zhswl 24 28 ] ] -RRB- cord-312431-de7zhswl 24 29 . . . cord-312431-de7zhswl 25 1 Phages phage NNS cord-312431-de7zhswl 25 2 share share VBP cord-312431-de7zhswl 25 3 many many JJ cord-312431-de7zhswl 25 4 properties property NNS cord-312431-de7zhswl 25 5 with with IN cord-312431-de7zhswl 25 6 human human JJ cord-312431-de7zhswl 25 7 viruses virus NNS cord-312431-de7zhswl 25 8 , , , cord-312431-de7zhswl 25 9 particularly particularly RB cord-312431-de7zhswl 25 10 composition composition NN cord-312431-de7zhswl 25 11 , , , cord-312431-de7zhswl 25 12 morphology morphology NN cord-312431-de7zhswl 25 13 and and CC cord-312431-de7zhswl 25 14 structure structure NN cord-312431-de7zhswl 25 15 [ [ -LRB- cord-312431-de7zhswl 25 16 15 15 CD cord-312431-de7zhswl 25 17 , , , cord-312431-de7zhswl 25 18 16 16 CD cord-312431-de7zhswl 25 19 ] ] -RRB- cord-312431-de7zhswl 25 20 . . . cord-312431-de7zhswl 26 1 Phages phage NNS cord-312431-de7zhswl 26 2 , , , cord-312431-de7zhswl 26 3 especially especially RB cord-312431-de7zhswl 26 4 somatic somatic JJ cord-312431-de7zhswl 26 5 coliphages coliphage NNS cord-312431-de7zhswl 26 6 and and CC cord-312431-de7zhswl 26 7 F F NNP cord-312431-de7zhswl 26 8 - - HYPH cord-312431-de7zhswl 26 9 RNA RNA NNP cord-312431-de7zhswl 26 10 coliphages coliphage NNS cord-312431-de7zhswl 26 11 , , , cord-312431-de7zhswl 26 12 have have VBP cord-312431-de7zhswl 26 13 been be VBN cord-312431-de7zhswl 26 14 recommended recommend VBN cord-312431-de7zhswl 26 15 as as IN cord-312431-de7zhswl 26 16 alternate alternate JJ cord-312431-de7zhswl 26 17 microbial microbial JJ cord-312431-de7zhswl 26 18 indicators indicator NNS cord-312431-de7zhswl 26 19 as as IN cord-312431-de7zhswl 26 20 they -PRON- PRP cord-312431-de7zhswl 26 21 behave behave VBP cord-312431-de7zhswl 26 22 more more RBR cord-312431-de7zhswl 26 23 like like IN cord-312431-de7zhswl 26 24 the the DT cord-312431-de7zhswl 26 25 human human JJ cord-312431-de7zhswl 26 26 enteric enteric JJ cord-312431-de7zhswl 26 27 viruses virus NNS cord-312431-de7zhswl 26 28 which which WDT cord-312431-de7zhswl 26 29 pose pose VBP cord-312431-de7zhswl 26 30 a a DT cord-312431-de7zhswl 26 31 health health NN cord-312431-de7zhswl 26 32 risk risk NN cord-312431-de7zhswl 26 33 to to TO cord-312431-de7zhswl 26 34 water water NN cord-312431-de7zhswl 26 35 consumers consumer NNS cord-312431-de7zhswl 26 36 if if IN cord-312431-de7zhswl 26 37 water water NN cord-312431-de7zhswl 26 38 has have VBZ cord-312431-de7zhswl 26 39 been be VBN cord-312431-de7zhswl 26 40 contaminated contaminate VBN cord-312431-de7zhswl 26 41 with with IN cord-312431-de7zhswl 26 42 human human JJ cord-312431-de7zhswl 26 43 faeces faece NNS cord-312431-de7zhswl 26 44 [ [ -LRB- cord-312431-de7zhswl 26 45 17 17 CD cord-312431-de7zhswl 26 46 , , , cord-312431-de7zhswl 26 47 18 18 CD cord-312431-de7zhswl 26 48 ] ] -RRB- cord-312431-de7zhswl 26 49 . . . cord-312431-de7zhswl 27 1 Somatic somatic JJ cord-312431-de7zhswl 27 2 coliphages coliphage NNS cord-312431-de7zhswl 27 3 have have VBP cord-312431-de7zhswl 27 4 been be VBN cord-312431-de7zhswl 27 5 described describe VBN cord-312431-de7zhswl 27 6 as as IN cord-312431-de7zhswl 27 7 being be VBG cord-312431-de7zhswl 27 8 a a DT cord-312431-de7zhswl 27 9 heterogeneous heterogeneous JJ cord-312431-de7zhswl 27 10 group group NN cord-312431-de7zhswl 27 11 of of IN cord-312431-de7zhswl 27 12 viruses virus NNS cord-312431-de7zhswl 27 13 , , , cord-312431-de7zhswl 27 14 which which WDT cord-312431-de7zhswl 27 15 could could MD cord-312431-de7zhswl 27 16 originate originate VB cord-312431-de7zhswl 27 17 from from IN cord-312431-de7zhswl 27 18 faecal faecal JJ cord-312431-de7zhswl 27 19 sources source NNS cord-312431-de7zhswl 27 20 [ [ -LRB- cord-312431-de7zhswl 27 21 17 17 CD cord-312431-de7zhswl 27 22 , , , cord-312431-de7zhswl 27 23 19 19 CD cord-312431-de7zhswl 27 24 ] ] -RRB- cord-312431-de7zhswl 27 25 . . . cord-312431-de7zhswl 28 1 The the DT cord-312431-de7zhswl 28 2 presence presence NN cord-312431-de7zhswl 28 3 of of IN cord-312431-de7zhswl 28 4 these these DT cord-312431-de7zhswl 28 5 viruses virus NNS cord-312431-de7zhswl 28 6 in in IN cord-312431-de7zhswl 28 7 faecally faecally RB cord-312431-de7zhswl 28 8 contaminated contaminate VBN cord-312431-de7zhswl 28 9 water water NN cord-312431-de7zhswl 28 10 means mean VBZ cord-312431-de7zhswl 28 11 that that IN cord-312431-de7zhswl 28 12 they -PRON- PRP cord-312431-de7zhswl 28 13 can can MD cord-312431-de7zhswl 28 14 serve serve VB cord-312431-de7zhswl 28 15 as as IN cord-312431-de7zhswl 28 16 indicators indicator NNS cord-312431-de7zhswl 28 17 of of IN cord-312431-de7zhswl 28 18 faecal faecal JJ cord-312431-de7zhswl 28 19 pollution pollution NN cord-312431-de7zhswl 28 20 and and CC cord-312431-de7zhswl 28 21 may may MD cord-312431-de7zhswl 28 22 indicate indicate VB cord-312431-de7zhswl 28 23 the the DT cord-312431-de7zhswl 28 24 coexisting coexist VBG cord-312431-de7zhswl 28 25 presence presence NN cord-312431-de7zhswl 28 26 of of IN cord-312431-de7zhswl 28 27 pathogenic pathogenic JJ cord-312431-de7zhswl 28 28 viruses virus NNS cord-312431-de7zhswl 28 29 . . . cord-312431-de7zhswl 29 1 Due due IN cord-312431-de7zhswl 29 2 to to IN cord-312431-de7zhswl 29 3 their -PRON- PRP$ cord-312431-de7zhswl 29 4 resistance resistance NN cord-312431-de7zhswl 29 5 against against IN cord-312431-de7zhswl 29 6 environmental environmental JJ cord-312431-de7zhswl 29 7 factors factor NNS cord-312431-de7zhswl 29 8 , , , cord-312431-de7zhswl 29 9 somatic somatic JJ cord-312431-de7zhswl 29 10 coliphages coliphage NNS cord-312431-de7zhswl 29 11 are be VBP cord-312431-de7zhswl 29 12 more more RBR cord-312431-de7zhswl 29 13 applicable applicable JJ cord-312431-de7zhswl 29 14 than than IN cord-312431-de7zhswl 29 15 faecal faecal JJ cord-312431-de7zhswl 29 16 bacteria bacteria NNS cord-312431-de7zhswl 29 17 for for IN cord-312431-de7zhswl 29 18 indicating indicate VBG cord-312431-de7zhswl 29 19 faecal faecal JJ cord-312431-de7zhswl 29 20 contamination contamination NN cord-312431-de7zhswl 29 21 of of IN cord-312431-de7zhswl 29 22 water water NN cord-312431-de7zhswl 29 23 [ [ -LRB- cord-312431-de7zhswl 29 24 15 15 CD cord-312431-de7zhswl 29 25 , , , cord-312431-de7zhswl 29 26 16 16 CD cord-312431-de7zhswl 29 27 , , , cord-312431-de7zhswl 29 28 20 20 CD cord-312431-de7zhswl 29 29 ] ] -RRB- cord-312431-de7zhswl 29 30 . . . cord-312431-de7zhswl 30 1 The the DT cord-312431-de7zhswl 30 2 occurrence occurrence NN cord-312431-de7zhswl 30 3 of of IN cord-312431-de7zhswl 30 4 F F NNP cord-312431-de7zhswl 30 5 - - HYPH cord-312431-de7zhswl 30 6 RNA RNA NNP cord-312431-de7zhswl 30 7 phages phage NNS cord-312431-de7zhswl 30 8 in in IN cord-312431-de7zhswl 30 9 surface surface NN cord-312431-de7zhswl 30 10 waters water NNS cord-312431-de7zhswl 30 11 generally generally RB cord-312431-de7zhswl 30 12 indicates indicate VBZ cord-312431-de7zhswl 30 13 pollution pollution NN cord-312431-de7zhswl 30 14 by by IN cord-312431-de7zhswl 30 15 human human JJ cord-312431-de7zhswl 30 16 or or CC cord-312431-de7zhswl 30 17 animal animal NN cord-312431-de7zhswl 30 18 faeces faece NNS cord-312431-de7zhswl 30 19 [ [ -LRB- cord-312431-de7zhswl 30 20 20 20 CD cord-312431-de7zhswl 30 21 ] ] -RRB- cord-312431-de7zhswl 30 22 [ [ -LRB- cord-312431-de7zhswl 30 23 21 21 CD cord-312431-de7zhswl 30 24 ] ] -RRB- cord-312431-de7zhswl 30 25 [ [ -LRB- cord-312431-de7zhswl 30 26 22 22 CD cord-312431-de7zhswl 30 27 ] ] -RRB- cord-312431-de7zhswl 30 28 [ [ -LRB- cord-312431-de7zhswl 30 29 23 23 CD cord-312431-de7zhswl 30 30 ] ] -RRB- cord-312431-de7zhswl 30 31 . . . cord-312431-de7zhswl 31 1 It -PRON- PRP cord-312431-de7zhswl 31 2 was be VBD cord-312431-de7zhswl 31 3 shown show VBN cord-312431-de7zhswl 31 4 that that IN cord-312431-de7zhswl 31 5 for for IN cord-312431-de7zhswl 31 6 monitoring monitoring NN cord-312431-de7zhswl 31 7 purposes purpose NNS cord-312431-de7zhswl 31 8 , , , cord-312431-de7zhswl 31 9 F f NN cord-312431-de7zhswl 31 10 - - HYPH cord-312431-de7zhswl 31 11 specific specific JJ cord-312431-de7zhswl 31 12 RNA RNA NNP cord-312431-de7zhswl 31 13 bacteriophages bacteriophage NNS cord-312431-de7zhswl 31 14 can can MD cord-312431-de7zhswl 31 15 indicate indicate VB cord-312431-de7zhswl 31 16 the the DT cord-312431-de7zhswl 31 17 possible possible JJ cord-312431-de7zhswl 31 18 presence presence NN cord-312431-de7zhswl 31 19 of of IN cord-312431-de7zhswl 31 20 human human JJ cord-312431-de7zhswl 31 21 pathogenic pathogenic JJ cord-312431-de7zhswl 31 22 enteric enteric JJ cord-312431-de7zhswl 31 23 viruses virus NNS cord-312431-de7zhswl 31 24 as as IN cord-312431-de7zhswl 31 25 they -PRON- PRP cord-312431-de7zhswl 31 26 behave behave VBP cord-312431-de7zhswl 31 27 like like IN cord-312431-de7zhswl 31 28 waterborne waterborne JJ cord-312431-de7zhswl 31 29 viruses virus NNS cord-312431-de7zhswl 31 30 and and CC cord-312431-de7zhswl 31 31 their -PRON- PRP$ cord-312431-de7zhswl 31 32 morphology morphology NN cord-312431-de7zhswl 31 33 and and CC cord-312431-de7zhswl 31 34 survival survival NN cord-312431-de7zhswl 31 35 characteristics characteristic NNS cord-312431-de7zhswl 31 36 closely closely RB cord-312431-de7zhswl 31 37 resemble resemble VBP cord-312431-de7zhswl 31 38 that that DT cord-312431-de7zhswl 31 39 of of IN cord-312431-de7zhswl 31 40 some some DT cord-312431-de7zhswl 31 41 of of IN cord-312431-de7zhswl 31 42 the the DT cord-312431-de7zhswl 31 43 important important JJ cord-312431-de7zhswl 31 44 human human JJ cord-312431-de7zhswl 31 45 enteric enteric JJ cord-312431-de7zhswl 31 46 viruses virus NNS cord-312431-de7zhswl 31 47 [ [ -LRB- cord-312431-de7zhswl 31 48 17 17 CD cord-312431-de7zhswl 31 49 , , , cord-312431-de7zhswl 31 50 24 24 CD cord-312431-de7zhswl 31 51 , , , cord-312431-de7zhswl 31 52 25 25 CD cord-312431-de7zhswl 31 53 ] ] -RRB- cord-312431-de7zhswl 31 54 . . . cord-312431-de7zhswl 32 1 The the DT cord-312431-de7zhswl 32 2 phage phage NN cord-312431-de7zhswl 32 3 plaque plaque NN cord-312431-de7zhswl 32 4 assay assay NN cord-312431-de7zhswl 32 5 has have VBZ cord-312431-de7zhswl 32 6 the the DT cord-312431-de7zhswl 32 7 advantages advantage NNS cord-312431-de7zhswl 32 8 of of IN cord-312431-de7zhswl 32 9 technical technical JJ cord-312431-de7zhswl 32 10 simplicity simplicity NN cord-312431-de7zhswl 32 11 and and CC cord-312431-de7zhswl 32 12 low low JJ cord-312431-de7zhswl 32 13 cost cost NN cord-312431-de7zhswl 32 14 but but CC cord-312431-de7zhswl 32 15 the the DT cord-312431-de7zhswl 32 16 choice choice NN cord-312431-de7zhswl 32 17 of of IN cord-312431-de7zhswl 32 18 a a DT cord-312431-de7zhswl 32 19 suitable suitable JJ cord-312431-de7zhswl 32 20 bacterial bacterial JJ cord-312431-de7zhswl 32 21 host host NN cord-312431-de7zhswl 32 22 is be VBZ cord-312431-de7zhswl 32 23 of of IN cord-312431-de7zhswl 32 24 paramount paramount JJ cord-312431-de7zhswl 32 25 importance importance NN cord-312431-de7zhswl 32 26 [ [ -LRB- cord-312431-de7zhswl 32 27 26 26 CD cord-312431-de7zhswl 32 28 ] ] -RRB- cord-312431-de7zhswl 32 29 . . . cord-312431-de7zhswl 33 1 One one CD cord-312431-de7zhswl 33 2 of of IN cord-312431-de7zhswl 33 3 the the DT cord-312431-de7zhswl 33 4 requirements requirement NNS cord-312431-de7zhswl 33 5 for for IN cord-312431-de7zhswl 33 6 the the DT cord-312431-de7zhswl 33 7 virological virological JJ cord-312431-de7zhswl 33 8 analysis analysis NN cord-312431-de7zhswl 33 9 of of IN cord-312431-de7zhswl 33 10 water water NN cord-312431-de7zhswl 33 11 is be VBZ cord-312431-de7zhswl 33 12 an an DT cord-312431-de7zhswl 33 13 efficient efficient JJ cord-312431-de7zhswl 33 14 and and CC cord-312431-de7zhswl 33 15 simple simple JJ cord-312431-de7zhswl 33 16 virus virus NN cord-312431-de7zhswl 33 17 concentration concentration NN cord-312431-de7zhswl 33 18 method method NN cord-312431-de7zhswl 33 19 . . . cord-312431-de7zhswl 34 1 It -PRON- PRP cord-312431-de7zhswl 34 2 is be VBZ cord-312431-de7zhswl 34 3 well well RB cord-312431-de7zhswl 34 4 recognised recognise VBN cord-312431-de7zhswl 34 5 that that IN cord-312431-de7zhswl 34 6 monitoring monitor VBG cord-312431-de7zhswl 34 7 the the DT cord-312431-de7zhswl 34 8 presence presence NN cord-312431-de7zhswl 34 9 of of IN cord-312431-de7zhswl 34 10 enteric enteric JJ cord-312431-de7zhswl 34 11 viruses virus NNS cord-312431-de7zhswl 34 12 could could MD cord-312431-de7zhswl 34 13 be be VB cord-312431-de7zhswl 34 14 challenging challenge VBG cord-312431-de7zhswl 34 15 due due IN cord-312431-de7zhswl 34 16 to to IN cord-312431-de7zhswl 34 17 the the DT cord-312431-de7zhswl 34 18 relatively relatively RB cord-312431-de7zhswl 34 19 low low JJ cord-312431-de7zhswl 34 20 level level NN cord-312431-de7zhswl 34 21 of of IN cord-312431-de7zhswl 34 22 infectious infectious JJ cord-312431-de7zhswl 34 23 viral viral JJ cord-312431-de7zhswl 34 24 particles particle NNS cord-312431-de7zhswl 34 25 towards towards IN cord-312431-de7zhswl 34 26 the the DT cord-312431-de7zhswl 34 27 respective respective JJ cord-312431-de7zhswl 34 28 host host NN cord-312431-de7zhswl 34 29 species specie NNS cord-312431-de7zhswl 34 30 and and CC cord-312431-de7zhswl 34 31 small small JJ cord-312431-de7zhswl 34 32 viral viral JJ cord-312431-de7zhswl 34 33 particle particle NN cord-312431-de7zhswl 34 34 size size NN cord-312431-de7zhswl 34 35 existing exist VBG cord-312431-de7zhswl 34 36 in in IN cord-312431-de7zhswl 34 37 environmental environmental JJ cord-312431-de7zhswl 34 38 waters water NNS cord-312431-de7zhswl 34 39 , , , cord-312431-de7zhswl 34 40 thus thus RB cord-312431-de7zhswl 34 41 making make VBG cord-312431-de7zhswl 34 42 it -PRON- PRP cord-312431-de7zhswl 34 43 essential essential JJ cord-312431-de7zhswl 34 44 to to TO cord-312431-de7zhswl 34 45 start start VB cord-312431-de7zhswl 34 46 with with IN cord-312431-de7zhswl 34 47 a a DT cord-312431-de7zhswl 34 48 large large JJ cord-312431-de7zhswl 34 49 water water NN cord-312431-de7zhswl 34 50 sample sample NN cord-312431-de7zhswl 34 51 volume volume NN cord-312431-de7zhswl 34 52 and and CC cord-312431-de7zhswl 34 53 concentrate concentrate VB cord-312431-de7zhswl 34 54 it -PRON- PRP cord-312431-de7zhswl 34 55 to to IN cord-312431-de7zhswl 34 56 several several JJ cord-312431-de7zhswl 34 57 orders order NNS cord-312431-de7zhswl 34 58 of of IN cord-312431-de7zhswl 34 59 magnitude magnitude NN cord-312431-de7zhswl 34 60 [ [ -LRB- cord-312431-de7zhswl 34 61 27 27 CD cord-312431-de7zhswl 34 62 ] ] -RRB- cord-312431-de7zhswl 34 63 [ [ -LRB- cord-312431-de7zhswl 34 64 28 28 CD cord-312431-de7zhswl 34 65 ] ] -RRB- cord-312431-de7zhswl 34 66 [ [ -LRB- cord-312431-de7zhswl 34 67 29 29 CD cord-312431-de7zhswl 34 68 ] ] -RRB- cord-312431-de7zhswl 34 69 [ [ -LRB- cord-312431-de7zhswl 34 70 30 30 CD cord-312431-de7zhswl 34 71 ] ] -RRB- cord-312431-de7zhswl 34 72 [ [ -LRB- cord-312431-de7zhswl 34 73 31 31 CD cord-312431-de7zhswl 34 74 ] ] -RRB- cord-312431-de7zhswl 34 75 . . . cord-312431-de7zhswl 35 1 However however RB cord-312431-de7zhswl 35 2 , , , cord-312431-de7zhswl 35 3 this this DT cord-312431-de7zhswl 35 4 limitation limitation NN cord-312431-de7zhswl 35 5 can can MD cord-312431-de7zhswl 35 6 be be VB cord-312431-de7zhswl 35 7 overcome overcome VBN cord-312431-de7zhswl 35 8 by by IN cord-312431-de7zhswl 35 9 the the DT cord-312431-de7zhswl 35 10 use use NN cord-312431-de7zhswl 35 11 of of IN cord-312431-de7zhswl 35 12 improved improved JJ cord-312431-de7zhswl 35 13 methods method NNS cord-312431-de7zhswl 35 14 for for IN cord-312431-de7zhswl 35 15 aquatic aquatic JJ cord-312431-de7zhswl 35 16 sample sample NN cord-312431-de7zhswl 35 17 concentration concentration NN cord-312431-de7zhswl 35 18 , , , cord-312431-de7zhswl 35 19 viral viral JJ cord-312431-de7zhswl 35 20 infectivity infectivity NN cord-312431-de7zhswl 35 21 and and CC cord-312431-de7zhswl 35 22 more more RBR cord-312431-de7zhswl 35 23 sensitive sensitive JJ cord-312431-de7zhswl 35 24 viral viral JJ cord-312431-de7zhswl 35 25 detection detection NN cord-312431-de7zhswl 35 26 techniques technique NNS cord-312431-de7zhswl 35 27 [ [ -LRB- cord-312431-de7zhswl 35 28 32 32 CD cord-312431-de7zhswl 35 29 ] ] -RRB- cord-312431-de7zhswl 35 30 . . . cord-312431-de7zhswl 36 1 Tangential tangential JJ cord-312431-de7zhswl 36 2 - - HYPH cord-312431-de7zhswl 36 3 flow flow NN cord-312431-de7zhswl 36 4 and and CC cord-312431-de7zhswl 36 5 hollow hollow JJ cord-312431-de7zhswl 36 6 - - HYPH cord-312431-de7zhswl 36 7 fibre fibre NN cord-312431-de7zhswl 36 8 ultrafiltration ultrafiltration NN cord-312431-de7zhswl 36 9 ( ( -LRB- cord-312431-de7zhswl 36 10 UF UF NNP cord-312431-de7zhswl 36 11 ) ) -RRB- cord-312431-de7zhswl 36 12 have have VBP cord-312431-de7zhswl 36 13 been be VBN cord-312431-de7zhswl 36 14 used use VBN cord-312431-de7zhswl 36 15 to to TO cord-312431-de7zhswl 36 16 investigate investigate VB cord-312431-de7zhswl 36 17 microbial microbial JJ cord-312431-de7zhswl 36 18 contamination contamination NN cord-312431-de7zhswl 36 19 of of IN cord-312431-de7zhswl 36 20 drinking drink VBG cord-312431-de7zhswl 36 21 water water NN cord-312431-de7zhswl 36 22 [ [ -LRB- cord-312431-de7zhswl 36 23 33 33 CD cord-312431-de7zhswl 36 24 , , , cord-312431-de7zhswl 36 25 34 34 CD cord-312431-de7zhswl 36 26 ] ] -RRB- cord-312431-de7zhswl 36 27 . . . cord-312431-de7zhswl 37 1 The the DT cord-312431-de7zhswl 37 2 virus virus NN cord-312431-de7zhswl 37 3 concentration concentration NN cord-312431-de7zhswl 37 4 method method NN cord-312431-de7zhswl 37 5 should should MD cord-312431-de7zhswl 37 6 be be VB cord-312431-de7zhswl 37 7 applicable applicable JJ cord-312431-de7zhswl 37 8 to to IN cord-312431-de7zhswl 37 9 a a DT cord-312431-de7zhswl 37 10 broad broad JJ cord-312431-de7zhswl 37 11 spectrum spectrum NN cord-312431-de7zhswl 37 12 of of IN cord-312431-de7zhswl 37 13 enteric enteric JJ cord-312431-de7zhswl 37 14 viruses virus NNS cord-312431-de7zhswl 37 15 to to TO cord-312431-de7zhswl 37 16 facilitate facilitate VB cord-312431-de7zhswl 37 17 concurrent concurrent JJ cord-312431-de7zhswl 37 18 downstream downstream JJ cord-312431-de7zhswl 37 19 study study NN cord-312431-de7zhswl 37 20 [ [ -LRB- cord-312431-de7zhswl 37 21 29 29 CD cord-312431-de7zhswl 37 22 ] ] -RRB- cord-312431-de7zhswl 37 23 . . . cord-312431-de7zhswl 38 1 The the DT cord-312431-de7zhswl 38 2 present present JJ cord-312431-de7zhswl 38 3 study study NN cord-312431-de7zhswl 38 4 was be VBD cord-312431-de7zhswl 38 5 conducted conduct VBN cord-312431-de7zhswl 38 6 to to TO cord-312431-de7zhswl 38 7 optimise optimise VB cord-312431-de7zhswl 38 8 procedures procedure NNS cord-312431-de7zhswl 38 9 to to TO cord-312431-de7zhswl 38 10 extract extract VB cord-312431-de7zhswl 38 11 and and CC cord-312431-de7zhswl 38 12 enumerate enumerate VB cord-312431-de7zhswl 38 13 indigenous indigenous JJ cord-312431-de7zhswl 38 14 virus virus NN cord-312431-de7zhswl 38 15 - - HYPH cord-312431-de7zhswl 38 16 like like JJ cord-312431-de7zhswl 38 17 particles particle NNS cord-312431-de7zhswl 38 18 ( ( -LRB- cord-312431-de7zhswl 38 19 VLPs vlp NNS cord-312431-de7zhswl 38 20 ) ) -RRB- cord-312431-de7zhswl 38 21 and and CC cord-312431-de7zhswl 38 22 to to TO cord-312431-de7zhswl 38 23 determine determine VB cord-312431-de7zhswl 38 24 the the DT cord-312431-de7zhswl 38 25 community community NN cord-312431-de7zhswl 38 26 structures structure NNS cord-312431-de7zhswl 38 27 and and CC cord-312431-de7zhswl 38 28 infectivity infectivity NN cord-312431-de7zhswl 38 29 of of IN cord-312431-de7zhswl 38 30 these these DT cord-312431-de7zhswl 38 31 viruses virus NNS cord-312431-de7zhswl 38 32 from from IN cord-312431-de7zhswl 38 33 river river NN cord-312431-de7zhswl 38 34 water water NN cord-312431-de7zhswl 38 35 . . . cord-312431-de7zhswl 39 1 A a DT cord-312431-de7zhswl 39 2 setup setup NN cord-312431-de7zhswl 39 3 for for IN cord-312431-de7zhswl 39 4 the the DT cord-312431-de7zhswl 39 5 concentration concentration NN cord-312431-de7zhswl 39 6 of of IN cord-312431-de7zhswl 39 7 viruses virus NNS cord-312431-de7zhswl 39 8 from from IN cord-312431-de7zhswl 39 9 large large JJ cord-312431-de7zhswl 39 10 ( ( -LRB- cord-312431-de7zhswl 39 11 20 20 CD cord-312431-de7zhswl 39 12 L l NN cord-312431-de7zhswl 39 13 ) ) -RRB- cord-312431-de7zhswl 39 14 volume volume NN cord-312431-de7zhswl 39 15 water water NN cord-312431-de7zhswl 39 16 samples sample NNS cord-312431-de7zhswl 39 17 using use VBG cord-312431-de7zhswl 39 18 a a DT cord-312431-de7zhswl 39 19 two two CD cord-312431-de7zhswl 39 20 - - HYPH cord-312431-de7zhswl 39 21 step step NN cord-312431-de7zhswl 39 22 tangential tangential JJ cord-312431-de7zhswl 39 23 flow flow NN cord-312431-de7zhswl 39 24 filtration filtration NN cord-312431-de7zhswl 39 25 ( ( -LRB- cord-312431-de7zhswl 39 26 TFF TFF NNP cord-312431-de7zhswl 39 27 ) ) -RRB- cord-312431-de7zhswl 39 28 process process NN cord-312431-de7zhswl 39 29 was be VBD cord-312431-de7zhswl 39 30 established establish VBN cord-312431-de7zhswl 39 31 . . . cord-312431-de7zhswl 40 1 The the DT cord-312431-de7zhswl 40 2 presence presence NN cord-312431-de7zhswl 40 3 of of IN cord-312431-de7zhswl 40 4 VLPs vlp NNS cord-312431-de7zhswl 40 5 was be VBD cord-312431-de7zhswl 40 6 determined determine VBN cord-312431-de7zhswl 40 7 using use VBG cord-312431-de7zhswl 40 8 epifluorescence epifluorescence NN cord-312431-de7zhswl 40 9 microscopy microscopy NNP cord-312431-de7zhswl 40 10 ( ( -LRB- cord-312431-de7zhswl 40 11 EM EM NNP cord-312431-de7zhswl 40 12 ) ) -RRB- cord-312431-de7zhswl 40 13 , , , cord-312431-de7zhswl 40 14 transmission transmission NN cord-312431-de7zhswl 40 15 electron electron NN cord-312431-de7zhswl 40 16 microscopy microscopy NN cord-312431-de7zhswl 40 17 ( ( -LRB- cord-312431-de7zhswl 40 18 TEM TEM NNP cord-312431-de7zhswl 40 19 ) ) -RRB- cord-312431-de7zhswl 40 20 and and CC cord-312431-de7zhswl 40 21 their -PRON- PRP$ cord-312431-de7zhswl 40 22 infectivity infectivity NN cord-312431-de7zhswl 40 23 was be VBD cord-312431-de7zhswl 40 24 determined determine VBN cord-312431-de7zhswl 40 25 on on IN cord-312431-de7zhswl 40 26 cell cell NN cord-312431-de7zhswl 40 27 culture culture NN cord-312431-de7zhswl 40 28 . . . cord-312431-de7zhswl 41 1 Focus focus VB cord-312431-de7zhswl 41 2 in in IN cord-312431-de7zhswl 41 3 this this DT cord-312431-de7zhswl 41 4 study study NN cord-312431-de7zhswl 41 5 was be VBD cord-312431-de7zhswl 41 6 on on IN cord-312431-de7zhswl 41 7 the the DT cord-312431-de7zhswl 41 8 Umgeni Umgeni NNP cord-312431-de7zhswl 41 9 River River NNP cord-312431-de7zhswl 41 10 ( ( -LRB- cord-312431-de7zhswl 41 11 five five CD cord-312431-de7zhswl 41 12 points point NNS cord-312431-de7zhswl 41 13 along along IN cord-312431-de7zhswl 41 14 the the DT cord-312431-de7zhswl 41 15 river river NN cord-312431-de7zhswl 41 16 as as IN cord-312431-de7zhswl 41 17 shown show VBN cord-312431-de7zhswl 41 18 in in IN cord-312431-de7zhswl 41 19 Fig Fig NNP cord-312431-de7zhswl 41 20 . . . cord-312431-de7zhswl 42 1 1 1 LS cord-312431-de7zhswl 42 2 ) ) -RRB- cord-312431-de7zhswl 42 3 due due IN cord-312431-de7zhswl 42 4 to to IN cord-312431-de7zhswl 42 5 it -PRON- PRP cord-312431-de7zhswl 42 6 being be VBG cord-312431-de7zhswl 42 7 the the DT cord-312431-de7zhswl 42 8 largest large JJS cord-312431-de7zhswl 42 9 catchment catchment NN cord-312431-de7zhswl 42 10 ( ( -LRB- cord-312431-de7zhswl 42 11 5000 5000 CD cord-312431-de7zhswl 42 12 km km NN cord-312431-de7zhswl 42 13 2 2 CD cord-312431-de7zhswl 42 14 ) ) -RRB- cord-312431-de7zhswl 42 15 in in IN cord-312431-de7zhswl 42 16 the the DT cord-312431-de7zhswl 42 17 KwaZulu KwaZulu NNP cord-312431-de7zhswl 42 18 - - HYPH cord-312431-de7zhswl 42 19 Natal natal JJ cord-312431-de7zhswl 42 20 region region NN cord-312431-de7zhswl 42 21 of of IN cord-312431-de7zhswl 42 22 South South NNP cord-312431-de7zhswl 42 23 Africa Africa NNP cord-312431-de7zhswl 42 24 . . . cord-312431-de7zhswl 43 1 The the DT cord-312431-de7zhswl 43 2 sampling sampling NN cord-312431-de7zhswl 43 3 seasons season NNS cord-312431-de7zhswl 43 4 and and CC cord-312431-de7zhswl 43 5 sites site NNS cord-312431-de7zhswl 43 6 included include VBD cord-312431-de7zhswl 43 7 five five CD cord-312431-de7zhswl 43 8 vast vast JJ cord-312431-de7zhswl 43 9 areas area NNS cord-312431-de7zhswl 43 10 of of IN cord-312431-de7zhswl 43 11 the the DT cord-312431-de7zhswl 43 12 river river NN cord-312431-de7zhswl 43 13 starting start VBG cord-312431-de7zhswl 43 14 from from IN cord-312431-de7zhswl 43 15 a a DT cord-312431-de7zhswl 43 16 Dam Dam NNP cord-312431-de7zhswl 43 17 water water NN cord-312431-de7zhswl 43 18 source source NN cord-312431-de7zhswl 43 19 , , , cord-312431-de7zhswl 43 20 passing pass VBG cord-312431-de7zhswl 43 21 through through RB cord-312431-de7zhswl 43 22 : : : cord-312431-de7zhswl 43 23 a a DT cord-312431-de7zhswl 43 24 nature nature NN cord-312431-de7zhswl 43 25 reserve reserve NN cord-312431-de7zhswl 43 26 , , , cord-312431-de7zhswl 43 27 waste waste NN cord-312431-de7zhswl 43 28 treatment treatment NN cord-312431-de7zhswl 43 29 works work NNS cord-312431-de7zhswl 43 30 , , , cord-312431-de7zhswl 43 31 informal informal JJ cord-312431-de7zhswl 43 32 human human JJ cord-312431-de7zhswl 43 33 settlement settlement NN cord-312431-de7zhswl 43 34 and and CC cord-312431-de7zhswl 43 35 finally finally RB cord-312431-de7zhswl 43 36 ending end VBG cord-312431-de7zhswl 43 37 of of IN cord-312431-de7zhswl 43 38 at at IN cord-312431-de7zhswl 43 39 the the DT cord-312431-de7zhswl 43 40 river river NN cord-312431-de7zhswl 43 41 mouth mouth NN cord-312431-de7zhswl 43 42 ( ( -LRB- cord-312431-de7zhswl 43 43 were be VBD cord-312431-de7zhswl 43 44 the the DT cord-312431-de7zhswl 43 45 river river NN cord-312431-de7zhswl 43 46 and and CC cord-312431-de7zhswl 43 47 ocean ocean NN cord-312431-de7zhswl 43 48 meet meet NNP cord-312431-de7zhswl 43 49 ) ) -RRB- cord-312431-de7zhswl 43 50 . . . cord-312431-de7zhswl 44 1 Sampling sampling NN cord-312431-de7zhswl 44 2 points point NNS cord-312431-de7zhswl 44 3 were be VBD cord-312431-de7zhswl 44 4 designated designate VBN cord-312431-de7zhswl 44 5 as as IN cord-312431-de7zhswl 44 6 follows follow VBZ cord-312431-de7zhswl 44 7 : : : cord-312431-de7zhswl 44 8 U1 U1 NNP cord-312431-de7zhswl 44 9 ( ( -LRB- cord-312431-de7zhswl 44 10 Umgeni Umgeni NNP cord-312431-de7zhswl 44 11 River River NNP cord-312431-de7zhswl 44 12 Mouth Mouth NNP cord-312431-de7zhswl 44 13 - - HYPH cord-312431-de7zhswl 44 14 estuarine estuarine JJ cord-312431-de7zhswl 44 15 / / SYM cord-312431-de7zhswl 44 16 brackish brackish JJ cord-312431-de7zhswl 44 17 water water NN cord-312431-de7zhswl 44 18 ) ) -RRB- cord-312431-de7zhswl 44 19 , , , cord-312431-de7zhswl 44 20 U2 U2 NNP cord-312431-de7zhswl 44 21 ( ( -LRB- cord-312431-de7zhswl 44 22 Reservoir Reservoir NNP cord-312431-de7zhswl 44 23 Hills Hills NNPS cord-312431-de7zhswl 44 24 - - HYPH cord-312431-de7zhswl 44 25 informal informal JJ cord-312431-de7zhswl 44 26 settlement settlement NN cord-312431-de7zhswl 44 27 / / SYM cord-312431-de7zhswl 44 28 domestic domestic JJ cord-312431-de7zhswl 44 29 waste waste NN cord-312431-de7zhswl 44 30 ) ) -RRB- cord-312431-de7zhswl 44 31 , , , cord-312431-de7zhswl 44 32 U3 U3 NNP cord-312431-de7zhswl 44 33 ( ( -LRB- cord-312431-de7zhswl 44 34 New New NNP cord-312431-de7zhswl 44 35 Germany Germany NNP cord-312431-de7zhswl 44 36 Wastewater Wastewater NNP cord-312431-de7zhswl 44 37 Works work VBZ cord-312431-de7zhswl 44 38 - - HYPH cord-312431-de7zhswl 44 39 treated treat VBN cord-312431-de7zhswl 44 40 water water NN cord-312431-de7zhswl 44 41 after after IN cord-312431-de7zhswl 44 42 chlorination chlorination NN cord-312431-de7zhswl 44 43 that that WDT cord-312431-de7zhswl 44 44 enters enter VBZ cord-312431-de7zhswl 44 45 the the DT cord-312431-de7zhswl 44 46 adjoining adjoin VBG cord-312431-de7zhswl 44 47 Umgeni Umgeni NNP cord-312431-de7zhswl 44 48 River River NNP cord-312431-de7zhswl 44 49 water water NN cord-312431-de7zhswl 44 50 ) ) -RRB- cord-312431-de7zhswl 44 51 , , , cord-312431-de7zhswl 44 52 U4 U4 NNP cord-312431-de7zhswl 44 53 ( ( -LRB- cord-312431-de7zhswl 44 54 Krantzkloof Krantzkloof NNP cord-312431-de7zhswl 44 55 Nature Nature NNP cord-312431-de7zhswl 44 56 Reserve Reserve NNP cord-312431-de7zhswl 44 57 - - HYPH cord-312431-de7zhswl 44 58 vegetation vegetation NN cord-312431-de7zhswl 44 59 and and CC cord-312431-de7zhswl 44 60 conservation conservation NN cord-312431-de7zhswl 44 61 area area NN cord-312431-de7zhswl 44 62 ) ) -RRB- cord-312431-de7zhswl 44 63 and and CC cord-312431-de7zhswl 44 64 U5 U5 NNP cord-312431-de7zhswl 44 65 ( ( -LRB- cord-312431-de7zhswl 44 66 Inanda Inanda NNP cord-312431-de7zhswl 44 67 Dam Dam NNP cord-312431-de7zhswl 44 68 - - HYPH cord-312431-de7zhswl 44 69 restricted restricted JJ cord-312431-de7zhswl 44 70 water water NN cord-312431-de7zhswl 44 71 containment containment NN cord-312431-de7zhswl 44 72 ) ) -RRB- cord-312431-de7zhswl 44 73 . . . cord-312431-de7zhswl 45 1 Water water NN cord-312431-de7zhswl 45 2 samples sample NNS cord-312431-de7zhswl 45 3 from from IN cord-312431-de7zhswl 45 4 the the DT cord-312431-de7zhswl 45 5 River River NNP cord-312431-de7zhswl 45 6 were be VBD cord-312431-de7zhswl 45 7 collected collect VBN cord-312431-de7zhswl 45 8 during during IN cord-312431-de7zhswl 45 9 autumn autumn NN cord-312431-de7zhswl 45 10 ( ( -LRB- cord-312431-de7zhswl 45 11 March March NNP cord-312431-de7zhswl 45 12 - - HYPH cord-312431-de7zhswl 45 13 April April NNP cord-312431-de7zhswl 45 14 2011 2011 CD cord-312431-de7zhswl 45 15 ) ) -RRB- cord-312431-de7zhswl 45 16 , , , cord-312431-de7zhswl 45 17 winter winter NN cord-312431-de7zhswl 45 18 ( ( -LRB- cord-312431-de7zhswl 45 19 June June NNP cord-312431-de7zhswl 45 20 - - HYPH cord-312431-de7zhswl 45 21 July July NNP cord-312431-de7zhswl 45 22 2011 2011 CD cord-312431-de7zhswl 45 23 ) ) -RRB- cord-312431-de7zhswl 45 24 , , , cord-312431-de7zhswl 45 25 spring spring NN cord-312431-de7zhswl 45 26 ( ( -LRB- cord-312431-de7zhswl 45 27 September September NNP cord-312431-de7zhswl 45 28 - - HYPH cord-312431-de7zhswl 45 29 October October NNP cord-312431-de7zhswl 45 30 2011 2011 CD cord-312431-de7zhswl 45 31 ) ) -RRB- cord-312431-de7zhswl 45 32 and and CC cord-312431-de7zhswl 45 33 summer summer NN cord-312431-de7zhswl 45 34 ( ( -LRB- cord-312431-de7zhswl 45 35 December December NNP cord-312431-de7zhswl 45 36 2011-January 2011-january CD cord-312431-de7zhswl 45 37 2012 2012 CD cord-312431-de7zhswl 45 38 ) ) -RRB- cord-312431-de7zhswl 45 39 months month NNS cord-312431-de7zhswl 45 40 , , , cord-312431-de7zhswl 45 41 to to TO cord-312431-de7zhswl 45 42 cover cover VB cord-312431-de7zhswl 45 43 the the DT cord-312431-de7zhswl 45 44 four four CD cord-312431-de7zhswl 45 45 seasons season NNS cord-312431-de7zhswl 45 46 of of IN cord-312431-de7zhswl 45 47 the the DT cord-312431-de7zhswl 45 48 year year NN cord-312431-de7zhswl 45 49 . . . cord-312431-de7zhswl 46 1 Water water NN cord-312431-de7zhswl 46 2 samples sample NNS cord-312431-de7zhswl 46 3 were be VBD cord-312431-de7zhswl 46 4 collected collect VBN cord-312431-de7zhswl 46 5 in in IN cord-312431-de7zhswl 46 6 25 25 CD cord-312431-de7zhswl 46 7 L l NN cord-312431-de7zhswl 46 8 plastic plastic NN cord-312431-de7zhswl 46 9 drums drum NNS cord-312431-de7zhswl 46 10 with with IN cord-312431-de7zhswl 46 11 caps cap NNS cord-312431-de7zhswl 46 12 . . . cord-312431-de7zhswl 47 1 Prior prior RB cord-312431-de7zhswl 47 2 to to IN cord-312431-de7zhswl 47 3 the the DT cord-312431-de7zhswl 47 4 sample sample NN cord-312431-de7zhswl 47 5 collections collection NNS cord-312431-de7zhswl 47 6 , , , cord-312431-de7zhswl 47 7 the the DT cord-312431-de7zhswl 47 8 alcohol alcohol NN cord-312431-de7zhswl 47 9 - - HYPH cord-312431-de7zhswl 47 10 sterilised sterilise VBN cord-312431-de7zhswl 47 11 drums drum NNS cord-312431-de7zhswl 47 12 were be VBD cord-312431-de7zhswl 47 13 rinsed rinse VBN cord-312431-de7zhswl 47 14 with with IN cord-312431-de7zhswl 47 15 the the DT cord-312431-de7zhswl 47 16 water water NN cord-312431-de7zhswl 47 17 from from IN cord-312431-de7zhswl 47 18 the the DT cord-312431-de7zhswl 47 19 river river NN cord-312431-de7zhswl 47 20 source source NN cord-312431-de7zhswl 47 21 . . . cord-312431-de7zhswl 48 1 The the DT cord-312431-de7zhswl 48 2 water water NN cord-312431-de7zhswl 48 3 samples sample NNS cord-312431-de7zhswl 48 4 were be VBD cord-312431-de7zhswl 48 5 collected collect VBN cord-312431-de7zhswl 48 6 knee knee NN cord-312431-de7zhswl 48 7 deep deep RB cord-312431-de7zhswl 49 1 ( ( -LRB- cord-312431-de7zhswl 49 2 AE0.5 AE0.5 NNP cord-312431-de7zhswl 49 3 m m NNP cord-312431-de7zhswl 49 4 ) ) -RRB- cord-312431-de7zhswl 49 5 below below IN cord-312431-de7zhswl 49 6 the the DT cord-312431-de7zhswl 49 7 water water NN cord-312431-de7zhswl 49 8 surface surface NN cord-312431-de7zhswl 49 9 facing face VBG cord-312431-de7zhswl 49 10 away away RB cord-312431-de7zhswl 49 11 from from IN cord-312431-de7zhswl 49 12 the the DT cord-312431-de7zhswl 49 13 water water NN cord-312431-de7zhswl 49 14 current current JJ cord-312431-de7zhswl 49 15 . . . cord-312431-de7zhswl 50 1 All all DT cord-312431-de7zhswl 50 2 samples sample NNS cord-312431-de7zhswl 50 3 were be VBD cord-312431-de7zhswl 50 4 protected protect VBN cord-312431-de7zhswl 50 5 from from IN cord-312431-de7zhswl 50 6 direct direct JJ cord-312431-de7zhswl 50 7 sunlight sunlight NN cord-312431-de7zhswl 50 8 and and CC cord-312431-de7zhswl 50 9 transported transport VBN cord-312431-de7zhswl 50 10 to to IN cord-312431-de7zhswl 50 11 the the DT cord-312431-de7zhswl 50 12 laboratory laboratory NN cord-312431-de7zhswl 50 13 within within IN cord-312431-de7zhswl 50 14 one one CD cord-312431-de7zhswl 50 15 hour hour NN cord-312431-de7zhswl 50 16 of of IN cord-312431-de7zhswl 50 17 collection collection NN cord-312431-de7zhswl 50 18 , , , cord-312431-de7zhswl 50 19 and and CC cord-312431-de7zhswl 50 20 stored store VBN cord-312431-de7zhswl 50 21 at at IN cord-312431-de7zhswl 50 22 4 4 CD cord-312431-de7zhswl 50 23 ° ° NN cord-312431-de7zhswl 50 24 C c NN cord-312431-de7zhswl 50 25 until until IN cord-312431-de7zhswl 50 26 further further JJ cord-312431-de7zhswl 50 27 analysis analysis NN cord-312431-de7zhswl 50 28 . . . cord-312431-de7zhswl 51 1 Escherichia escherichia NN cord-312431-de7zhswl 51 2 coli coli VBZ cord-312431-de7zhswl 51 3 ATCC ATCC NNP cord-312431-de7zhswl 51 4 13786 13786 CD cord-312431-de7zhswl 52 1 ( ( -LRB- cord-312431-de7zhswl 52 2 American american JJ cord-312431-de7zhswl 52 3 type type NN cord-312431-de7zhswl 52 4 culture culture NN cord-312431-de7zhswl 52 5 collection collection NN cord-312431-de7zhswl 52 6 ) ) -RRB- cord-312431-de7zhswl 52 7 was be VBD cord-312431-de7zhswl 52 8 used use VBN cord-312431-de7zhswl 52 9 as as IN cord-312431-de7zhswl 52 10 the the DT cord-312431-de7zhswl 52 11 host host NN cord-312431-de7zhswl 52 12 for for IN cord-312431-de7zhswl 52 13 the the DT cord-312431-de7zhswl 52 14 somatic somatic JJ cord-312431-de7zhswl 52 15 coliphage coliphage NN cord-312431-de7zhswl 52 16 . . . cord-312431-de7zhswl 53 1 The the DT cord-312431-de7zhswl 53 2 bacterial bacterial JJ cord-312431-de7zhswl 53 3 host host NN cord-312431-de7zhswl 53 4 was be VBD cord-312431-de7zhswl 53 5 grown grow VBN cord-312431-de7zhswl 53 6 overnight overnight RB cord-312431-de7zhswl 53 7 at at IN cord-312431-de7zhswl 53 8 37 37 CD cord-312431-de7zhswl 53 9 ° ° NN cord-312431-de7zhswl 53 10 C c NN cord-312431-de7zhswl 53 11 on on IN cord-312431-de7zhswl 53 12 a a DT cord-312431-de7zhswl 53 13 shaking shake VBG cord-312431-de7zhswl 53 14 incubator incubator NN cord-312431-de7zhswl 53 15 at at IN cord-312431-de7zhswl 53 16 100 100 CD cord-312431-de7zhswl 53 17 rpm rpm NN cord-312431-de7zhswl 53 18 , , , cord-312431-de7zhswl 53 19 in in IN cord-312431-de7zhswl 53 20 nutrient nutrient NN cord-312431-de7zhswl 53 21 broth broth NN cord-312431-de7zhswl 53 22 . . . cord-312431-de7zhswl 54 1 Salmonella salmonella NN cord-312431-de7zhswl 54 2 typhimurium typhimurium NN cord-312431-de7zhswl 54 3 WG49 WG49 NNP cord-312431-de7zhswl 54 4 ( ( -LRB- cord-312431-de7zhswl 54 5 provided provide VBN cord-312431-de7zhswl 54 6 by by IN cord-312431-de7zhswl 54 7 Dr. Dr. NNP cord-312431-de7zhswl 54 8 Maite Maite NNP cord-312431-de7zhswl 54 9 Muniesa Muniesa NNP cord-312431-de7zhswl 54 10 , , , cord-312431-de7zhswl 54 11 University University NNP cord-312431-de7zhswl 54 12 of of IN cord-312431-de7zhswl 54 13 Barcelona Barcelona NNP cord-312431-de7zhswl 54 14 , , , cord-312431-de7zhswl 54 15 Spain Spain NNP cord-312431-de7zhswl 54 16 ) ) -RRB- cord-312431-de7zhswl 54 17 was be VBD cord-312431-de7zhswl 54 18 used use VBN cord-312431-de7zhswl 54 19 as as IN cord-312431-de7zhswl 54 20 the the DT cord-312431-de7zhswl 54 21 host host NN cord-312431-de7zhswl 54 22 for for IN cord-312431-de7zhswl 54 23 F F NNP cord-312431-de7zhswl 54 24 - - HYPH cord-312431-de7zhswl 54 25 specific specific JJ cord-312431-de7zhswl 54 26 coliphage coliphage NN cord-312431-de7zhswl 54 27 . . . cord-312431-de7zhswl 55 1 The the DT cord-312431-de7zhswl 55 2 S. S. NNP cord-312431-de7zhswl 55 3 typhi typhi NN cord-312431-de7zhswl 55 4 host host NN cord-312431-de7zhswl 55 5 was be VBD cord-312431-de7zhswl 55 6 grown grow VBN cord-312431-de7zhswl 55 7 in in IN cord-312431-de7zhswl 55 8 Tryptone Tryptone NNP cord-312431-de7zhswl 55 9 Yeast Yeast NNP cord-312431-de7zhswl 55 10 Extract Extract NNP cord-312431-de7zhswl 55 11 broth broth NN cord-312431-de7zhswl 55 12 and and CC cord-312431-de7zhswl 55 13 incubated incubate VBN cord-312431-de7zhswl 55 14 at at IN cord-312431-de7zhswl 55 15 37 37 CD cord-312431-de7zhswl 55 16 ° ° NN cord-312431-de7zhswl 55 17 C c NN cord-312431-de7zhswl 55 18 with with IN cord-312431-de7zhswl 55 19 shaking shake VBG cord-312431-de7zhswl 55 20 at at IN cord-312431-de7zhswl 55 21 100 100 CD cord-312431-de7zhswl 55 22 rpm rpm NN cord-312431-de7zhswl 55 23 until until IN cord-312431-de7zhswl 55 24 the the DT cord-312431-de7zhswl 55 25 F F NNP cord-312431-de7zhswl 55 26 - - HYPH cord-312431-de7zhswl 55 27 pili pili NNP cord-312431-de7zhswl 55 28 developed develop VBD cord-312431-de7zhswl 55 29 ( ( -LRB- cord-312431-de7zhswl 55 30 ISO ISO NNP cord-312431-de7zhswl 55 31 1995 1995 CD cord-312431-de7zhswl 55 32 ) ) -RRB- cord-312431-de7zhswl 55 33 . . . cord-312431-de7zhswl 56 1 This this DT cord-312431-de7zhswl 56 2 growth growth NN cord-312431-de7zhswl 56 3 suspension suspension NN cord-312431-de7zhswl 56 4 was be VBD cord-312431-de7zhswl 56 5 monitored monitor VBN cord-312431-de7zhswl 56 6 at at IN cord-312431-de7zhswl 56 7 30 30 CD cord-312431-de7zhswl 56 8 min min NN cord-312431-de7zhswl 56 9 intervals interval NNS cord-312431-de7zhswl 56 10 from from IN cord-312431-de7zhswl 56 11 time time NN cord-312431-de7zhswl 56 12 0 0 CD cord-312431-de7zhswl 56 13 against against IN cord-312431-de7zhswl 56 14 a a DT cord-312431-de7zhswl 56 15 blank blank JJ cord-312431-de7zhswl 56 16 reference reference NN cord-312431-de7zhswl 56 17 at at IN cord-312431-de7zhswl 56 18 wavelength wavelength NN cord-312431-de7zhswl 56 19 of of IN cord-312431-de7zhswl 56 20 560 560 CD cord-312431-de7zhswl 56 21 nm nm NN cord-312431-de7zhswl 56 22 until until IN cord-312431-de7zhswl 56 23 an an DT cord-312431-de7zhswl 56 24 absorbance absorbance NN cord-312431-de7zhswl 56 25 of of IN cord-312431-de7zhswl 56 26 0.75 0.75 CD cord-312431-de7zhswl 56 27 was be VBD cord-312431-de7zhswl 56 28 obtained obtain VBN cord-312431-de7zhswl 56 29 indicating indicate VBG cord-312431-de7zhswl 56 30 that that IN cord-312431-de7zhswl 56 31 the the DT cord-312431-de7zhswl 56 32 sex sex NN cord-312431-de7zhswl 56 33 pili pili NNS cord-312431-de7zhswl 56 34 were be VBD cord-312431-de7zhswl 56 35 produced produce VBN cord-312431-de7zhswl 56 36 [ [ -LRB- cord-312431-de7zhswl 56 37 18 18 CD cord-312431-de7zhswl 56 38 ] ] -RRB- cord-312431-de7zhswl 56 39 . . . cord-312431-de7zhswl 57 1 Bacteriophages bacteriophage NNS cord-312431-de7zhswl 57 2 were be VBD cord-312431-de7zhswl 57 3 enumerated enumerate VBN cord-312431-de7zhswl 57 4 by by IN cord-312431-de7zhswl 57 5 the the DT cord-312431-de7zhswl 57 6 double double JJ cord-312431-de7zhswl 57 7 agar agar NNP cord-312431-de7zhswl 57 8 layer layer NN cord-312431-de7zhswl 57 9 technique technique NN cord-312431-de7zhswl 57 10 following follow VBG cord-312431-de7zhswl 57 11 the the DT cord-312431-de7zhswl 57 12 ISO ISO NNP cord-312431-de7zhswl 57 13 10705 10705 CD cord-312431-de7zhswl 57 14 - - SYM cord-312431-de7zhswl 57 15 2 2 CD cord-312431-de7zhswl 57 16 standard standard NN cord-312431-de7zhswl 57 17 ( ( -LRB- cord-312431-de7zhswl 57 18 ISO ISO NNP cord-312431-de7zhswl 57 19 2000 2000 CD cord-312431-de7zhswl 57 20 ) ) -RRB- cord-312431-de7zhswl 57 21 for for IN cord-312431-de7zhswl 57 22 enumeration enumeration NN cord-312431-de7zhswl 57 23 of of IN cord-312431-de7zhswl 57 24 somatic somatic JJ cord-312431-de7zhswl 57 25 coliphages coliphage NNS cord-312431-de7zhswl 57 26 and and CC cord-312431-de7zhswl 57 27 ISO ISO NNP cord-312431-de7zhswl 57 28 10705 10705 CD cord-312431-de7zhswl 57 29 - - HYPH cord-312431-de7zhswl 57 30 1 1 CD cord-312431-de7zhswl 57 31 ( ( -LRB- cord-312431-de7zhswl 57 32 ISO ISO NNP cord-312431-de7zhswl 57 33 1995 1995 CD cord-312431-de7zhswl 57 34 ) ) -RRB- cord-312431-de7zhswl 57 35 for for IN cord-312431-de7zhswl 57 36 enumeration enumeration NN cord-312431-de7zhswl 57 37 of of IN cord-312431-de7zhswl 57 38 F f NN cord-312431-de7zhswl 57 39 - - HYPH cord-312431-de7zhswl 57 40 specific specific JJ cord-312431-de7zhswl 57 41 RNA RNA NNP cord-312431-de7zhswl 57 42 bacteriophages bacteriophage NNS cord-312431-de7zhswl 57 43 ( ( -LRB- cord-312431-de7zhswl 57 44 F F NNP cord-312431-de7zhswl 57 45 - - HYPH cord-312431-de7zhswl 57 46 RNA RNA NNP cord-312431-de7zhswl 57 47 ) ) -RRB- cord-312431-de7zhswl 57 48 . . . cord-312431-de7zhswl 58 1 One one CD cord-312431-de7zhswl 58 2 hundred hundred CD cord-312431-de7zhswl 58 3 microliters microliter NNS cord-312431-de7zhswl 58 4 of of IN cord-312431-de7zhswl 58 5 the the DT cord-312431-de7zhswl 58 6 concentrated concentrated JJ cord-312431-de7zhswl 58 7 samples sample NNS cord-312431-de7zhswl 58 8 were be VBD cord-312431-de7zhswl 58 9 mixed mix VBN cord-312431-de7zhswl 58 10 with with IN cord-312431-de7zhswl 58 11 1 1 CD cord-312431-de7zhswl 58 12 mL ml NN cord-312431-de7zhswl 58 13 of of IN cord-312431-de7zhswl 58 14 overnight overnight JJ cord-312431-de7zhswl 58 15 bacterial bacterial JJ cord-312431-de7zhswl 58 16 host host NN cord-312431-de7zhswl 58 17 and and CC cord-312431-de7zhswl 58 18 8 8 CD cord-312431-de7zhswl 58 19 mL ml NN cord-312431-de7zhswl 58 20 of of IN cord-312431-de7zhswl 58 21 soft soft JJ cord-312431-de7zhswl 58 22 agar agar NN cord-312431-de7zhswl 58 23 and and CC cord-312431-de7zhswl 58 24 the the DT cord-312431-de7zhswl 58 25 mixture mixture NN cord-312431-de7zhswl 58 26 was be VBD cord-312431-de7zhswl 58 27 poured pour VBN cord-312431-de7zhswl 58 28 over over RP cord-312431-de7zhswl 58 29 agar agar JJ cord-312431-de7zhswl 58 30 bottom bottom NN cord-312431-de7zhswl 58 31 plates plate NNS cord-312431-de7zhswl 58 32 [ [ -LRB- cord-312431-de7zhswl 58 33 36 36 CD cord-312431-de7zhswl 58 34 ] ] -RRB- cord-312431-de7zhswl 58 35 . . . cord-312431-de7zhswl 59 1 Plaques plaque NNS cord-312431-de7zhswl 59 2 were be VBD cord-312431-de7zhswl 59 3 enumerated enumerate VBN cord-312431-de7zhswl 59 4 after after IN cord-312431-de7zhswl 59 5 12 12 CD cord-312431-de7zhswl 59 6 - - SYM cord-312431-de7zhswl 59 7 24 24 CD cord-312431-de7zhswl 59 8 h h NN cord-312431-de7zhswl 59 9 of of IN cord-312431-de7zhswl 59 10 Shown Shown NNP cord-312431-de7zhswl 59 11 are be VBP cord-312431-de7zhswl 59 12 the the DT cord-312431-de7zhswl 59 13 locations location NNS cord-312431-de7zhswl 59 14 approximate approximate JJ cord-312431-de7zhswl 59 15 sampling sampling NN cord-312431-de7zhswl 59 16 points point NNS cord-312431-de7zhswl 59 17 of of IN cord-312431-de7zhswl 59 18 the the DT cord-312431-de7zhswl 59 19 Umgeni Umgeni NNP cord-312431-de7zhswl 59 20 River River NNP cord-312431-de7zhswl 59 21 investigated investigate VBN cord-312431-de7zhswl 59 22 in in IN cord-312431-de7zhswl 59 23 this this DT cord-312431-de7zhswl 59 24 study study NN cord-312431-de7zhswl 59 25 [ [ -LRB- cord-312431-de7zhswl 59 26 35 35 CD cord-312431-de7zhswl 59 27 ] ] -RRB- cord-312431-de7zhswl 59 28 . . . cord-312431-de7zhswl 60 1 incubation incubation NN cord-312431-de7zhswl 60 2 at at IN cord-312431-de7zhswl 60 3 37 37 CD cord-312431-de7zhswl 60 4 ° ° NN cord-312431-de7zhswl 60 5 C c NN cord-312431-de7zhswl 60 6 and and CC cord-312431-de7zhswl 60 7 plaque plaque NN cord-312431-de7zhswl 60 8 forming form VBG cord-312431-de7zhswl 60 9 units unit NNS cord-312431-de7zhswl 60 10 ( ( -LRB- cord-312431-de7zhswl 60 11 pfu pfu NN cord-312431-de7zhswl 60 12 ) ) -RRB- cord-312431-de7zhswl 60 13 were be VBD cord-312431-de7zhswl 60 14 recorded record VBN cord-312431-de7zhswl 60 15 per per IN cord-312431-de7zhswl 60 16 millilitre millilitre NN cord-312431-de7zhswl 60 17 . . . cord-312431-de7zhswl 61 1 Viral viral JJ cord-312431-de7zhswl 61 2 community community NN cord-312431-de7zhswl 61 3 samples sample NNS cord-312431-de7zhswl 61 4 ( ( -LRB- cord-312431-de7zhswl 61 5 virioplankton virioplankton NNP cord-312431-de7zhswl 61 6 ) ) -RRB- cord-312431-de7zhswl 61 7 were be VBD cord-312431-de7zhswl 61 8 concentrated concentrate VBN cord-312431-de7zhswl 61 9 using use VBG cord-312431-de7zhswl 61 10 a a DT cord-312431-de7zhswl 61 11 two two CD cord-312431-de7zhswl 61 12 - - HYPH cord-312431-de7zhswl 61 13 step step NN cord-312431-de7zhswl 61 14 TFF tff NN cord-312431-de7zhswl 61 15 process process NN cord-312431-de7zhswl 61 16 as as IN cord-312431-de7zhswl 61 17 given give VBN cord-312431-de7zhswl 61 18 in in IN cord-312431-de7zhswl 61 19 Fig Fig NNP cord-312431-de7zhswl 61 20 . . NNP cord-312431-de7zhswl 61 21 2 2 CD cord-312431-de7zhswl 61 22 , , , cord-312431-de7zhswl 61 23 according accord VBG cord-312431-de7zhswl 61 24 to to IN cord-312431-de7zhswl 61 25 the the DT cord-312431-de7zhswl 61 26 method method NN cord-312431-de7zhswl 61 27 of of IN cord-312431-de7zhswl 61 28 Wommack Wommack NNP cord-312431-de7zhswl 61 29 et et NNP cord-312431-de7zhswl 61 30 al al NNP cord-312431-de7zhswl 61 31 . . . cord-312431-de7zhswl 62 1 [ [ -LRB- cord-312431-de7zhswl 62 2 37 37 CD cord-312431-de7zhswl 62 3 ] ] -RRB- cord-312431-de7zhswl 62 4 , , , cord-312431-de7zhswl 62 5 with with IN cord-312431-de7zhswl 62 6 some some DT cord-312431-de7zhswl 62 7 modifications modification NNS cord-312431-de7zhswl 62 8 . . . cord-312431-de7zhswl 63 1 Concentration concentration NN cord-312431-de7zhswl 63 2 of of IN cord-312431-de7zhswl 63 3 virus virus NN cord-312431-de7zhswl 63 4 particles particle NNS cord-312431-de7zhswl 63 5 from from IN cord-312431-de7zhswl 63 6 large large JJ cord-312431-de7zhswl 63 7 volume volume NN cord-312431-de7zhswl 63 8 water water NN cord-312431-de7zhswl 63 9 samples sample NNS cord-312431-de7zhswl 63 10 occurred occur VBD cord-312431-de7zhswl 63 11 in in IN cord-312431-de7zhswl 63 12 four four CD cord-312431-de7zhswl 63 13 steps step NNS cord-312431-de7zhswl 63 14 : : : cord-312431-de7zhswl 64 1 ( ( -LRB- cord-312431-de7zhswl 64 2 i i NN cord-312431-de7zhswl 64 3 ) ) -RRB- cord-312431-de7zhswl 64 4 twenty twenty CD cord-312431-de7zhswl 64 5 litres litre NNS cord-312431-de7zhswl 64 6 of of IN cord-312431-de7zhswl 64 7 river river NN cord-312431-de7zhswl 64 8 water water NN cord-312431-de7zhswl 64 9 were be VBD cord-312431-de7zhswl 64 10 first first RB cord-312431-de7zhswl 64 11 pumped pump VBN cord-312431-de7zhswl 64 12 through through IN cord-312431-de7zhswl 64 13 a a DT cord-312431-de7zhswl 64 14 25 25 CD cord-312431-de7zhswl 64 15 mm mm CD cord-312431-de7zhswl 64 16 string string NN cord-312431-de7zhswl 64 17 - - HYPH cord-312431-de7zhswl 64 18 wound wound NN cord-312431-de7zhswl 64 19 polypropylene polypropylene NN cord-312431-de7zhswl 64 20 sediment sediment NN cord-312431-de7zhswl 64 21 cartridge cartridge NN cord-312431-de7zhswl 64 22 filter filter NN cord-312431-de7zhswl 64 23 to to TO cord-312431-de7zhswl 64 24 remove remove VB cord-312431-de7zhswl 64 25 large large JJ cord-312431-de7zhswl 64 26 particles particle NNS cord-312431-de7zhswl 64 27 and and CC cord-312431-de7zhswl 64 28 cells cell NNS cord-312431-de7zhswl 64 29 at at IN cord-312431-de7zhswl 64 30 a a DT cord-312431-de7zhswl 64 31 flow flow NN cord-312431-de7zhswl 64 32 rate rate NN cord-312431-de7zhswl 64 33 of of IN cord-312431-de7zhswl 64 34 230 230 CD cord-312431-de7zhswl 64 35 mL ml NN cord-312431-de7zhswl 64 36 / / SYM cord-312431-de7zhswl 64 37 min min NN cord-312431-de7zhswl 64 38 ; ; , cord-312431-de7zhswl 64 39 ( ( -LRB- cord-312431-de7zhswl 64 40 ii ii NN cord-312431-de7zhswl 64 41 ) ) -RRB- cord-312431-de7zhswl 64 42 a a DT cord-312431-de7zhswl 64 43 peristaltic peristaltic JJ cord-312431-de7zhswl 64 44 pump pump NN cord-312431-de7zhswl 64 45 ( ( -LRB- cord-312431-de7zhswl 64 46 Masterflex Masterflex NNP cord-312431-de7zhswl 64 47 ) ) -RRB- cord-312431-de7zhswl 64 48 was be VBD cord-312431-de7zhswl 64 49 used use VBN cord-312431-de7zhswl 64 50 at at IN cord-312431-de7zhswl 64 51 a a DT cord-312431-de7zhswl 64 52 flow flow NN cord-312431-de7zhswl 64 53 rate rate NN cord-312431-de7zhswl 64 54 of of IN cord-312431-de7zhswl 64 55 530 530 CD cord-312431-de7zhswl 64 56 mL ml NN cord-312431-de7zhswl 64 57 / / SYM cord-312431-de7zhswl 64 58 min min NN cord-312431-de7zhswl 64 59 with with IN cord-312431-de7zhswl 64 60 pressure pressure NN cord-312431-de7zhswl 64 61 ( ( -LRB- cord-312431-de7zhswl 64 62 P p NN cord-312431-de7zhswl 64 63 in in IN cord-312431-de7zhswl 64 64 ¼ ¼ NNP cord-312431-de7zhswl 64 65 7 7 CD cord-312431-de7zhswl 64 66 psi psi NN cord-312431-de7zhswl 64 67 and and CC cord-312431-de7zhswl 64 68 P p NN cord-312431-de7zhswl 64 69 out out RB cord-312431-de7zhswl 64 70 ¼ ¼ CD cord-312431-de7zhswl 64 71 6 6 CD cord-312431-de7zhswl 64 72 psi psi NNS cord-312431-de7zhswl 64 73 ) ) -RRB- cord-312431-de7zhswl 64 74 to to TO cord-312431-de7zhswl 64 75 pump pump VB cord-312431-de7zhswl 64 76 the the DT cord-312431-de7zhswl 64 77 pre pre JJ cord-312431-de7zhswl 64 78 - - JJ cord-312431-de7zhswl 64 79 treated treated JJ cord-312431-de7zhswl 64 80 water water NN cord-312431-de7zhswl 64 81 samples sample NNS cord-312431-de7zhswl 64 82 through through IN cord-312431-de7zhswl 64 83 a a DT cord-312431-de7zhswl 64 84 0.5 0.5 CD cord-312431-de7zhswl 64 85 m m CD cord-312431-de7zhswl 64 86 2 2 CD cord-312431-de7zhswl 64 87 , , , cord-312431-de7zhswl 64 88 0.22 0.22 CD cord-312431-de7zhswl 64 89 mm mm NNP cord-312431-de7zhswl 64 90 Pellicon Pellicon NNP cord-312431-de7zhswl 64 91 TFF TFF NNP cord-312431-de7zhswl 64 92 cartridge cartridge NN cord-312431-de7zhswl 64 93 filter filter NN cord-312431-de7zhswl 64 94 ( ( -LRB- cord-312431-de7zhswl 64 95 microporous microporous JJ cord-312431-de7zhswl 64 96 filtration filtration NN cord-312431-de7zhswl 64 97 membrane membrane NN cord-312431-de7zhswl 64 98 ) ) -RRB- cord-312431-de7zhswl 65 1 ( ( -LRB- cord-312431-de7zhswl 65 2 Millipore Millipore NNP cord-312431-de7zhswl 65 3 ) ) -RRB- cord-312431-de7zhswl 66 1 to to TO cord-312431-de7zhswl 66 2 remove remove VB cord-312431-de7zhswl 66 3 all all DT cord-312431-de7zhswl 66 4 bacterial bacterial JJ cord-312431-de7zhswl 66 5 cells cell NNS cord-312431-de7zhswl 67 1 ; ; : cord-312431-de7zhswl 67 2 ( ( -LRB- cord-312431-de7zhswl 67 3 iii iii NN cord-312431-de7zhswl 67 4 ) ) -RRB- cord-312431-de7zhswl 67 5 viruses virus NNS cord-312431-de7zhswl 67 6 in in IN cord-312431-de7zhswl 67 7 the the DT cord-312431-de7zhswl 67 8 cell cell NN cord-312431-de7zhswl 67 9 - - HYPH cord-312431-de7zhswl 67 10 free free JJ cord-312431-de7zhswl 67 11 permeate permeate NN cord-312431-de7zhswl 67 12 was be VBD cord-312431-de7zhswl 67 13 then then RB cord-312431-de7zhswl 67 14 further further RB cord-312431-de7zhswl 67 15 concentrated concentrate VBN cord-312431-de7zhswl 67 16 to to IN cord-312431-de7zhswl 67 17 a a DT cord-312431-de7zhswl 67 18 final final JJ cord-312431-de7zhswl 67 19 volume volume NN cord-312431-de7zhswl 67 20 of of IN cord-312431-de7zhswl 67 21 500 500 CD cord-312431-de7zhswl 67 22 mL ml NN cord-312431-de7zhswl 67 23 using use VBG cord-312431-de7zhswl 67 24 10 10 CD cord-312431-de7zhswl 67 25 m m CD cord-312431-de7zhswl 67 26 2 2 CD cord-312431-de7zhswl 67 27 100-kDa 100-kda CD cord-312431-de7zhswl 67 28 spiralwound spiralwound NN cord-312431-de7zhswl 67 29 TFF tff NN cord-312431-de7zhswl 67 30 filter filter NN cord-312431-de7zhswl 67 31 ( ( -LRB- cord-312431-de7zhswl 67 32 Helicon Helicon NNP cord-312431-de7zhswl 67 33 , , , cord-312431-de7zhswl 67 34 Millipore Millipore NNP cord-312431-de7zhswl 67 35 ) ) -RRB- cord-312431-de7zhswl 67 36 ; ; : cord-312431-de7zhswl 67 37 ( ( -LRB- cord-312431-de7zhswl 67 38 iv iv LS cord-312431-de7zhswl 67 39 ) ) -RRB- cord-312431-de7zhswl 67 40 the the DT cord-312431-de7zhswl 67 41 retentate retentate NN cord-312431-de7zhswl 67 42 was be VBD cord-312431-de7zhswl 67 43 allowed allow VBN cord-312431-de7zhswl 67 44 to to TO cord-312431-de7zhswl 67 45 re re VB cord-312431-de7zhswl 67 46 - - VB cord-312431-de7zhswl 67 47 circulate circulate VB cord-312431-de7zhswl 67 48 through through IN cord-312431-de7zhswl 67 49 the the DT cord-312431-de7zhswl 67 50 two two CD cord-312431-de7zhswl 67 51 step step NN cord-312431-de7zhswl 67 52 system system NN cord-312431-de7zhswl 67 53 until until IN cord-312431-de7zhswl 67 54 only only RB cord-312431-de7zhswl 67 55 500 500 CD cord-312431-de7zhswl 67 56 mL ml NN cord-312431-de7zhswl 67 57 to to IN cord-312431-de7zhswl 67 58 1 1 CD cord-312431-de7zhswl 67 59 L l NN cord-312431-de7zhswl 67 60 of of IN cord-312431-de7zhswl 67 61 sample sample NN cord-312431-de7zhswl 67 62 remained remain VBD cord-312431-de7zhswl 67 63 in in IN cord-312431-de7zhswl 67 64 the the DT cord-312431-de7zhswl 67 65 original original JJ cord-312431-de7zhswl 67 66 vessel vessel NN cord-312431-de7zhswl 67 67 . . . cord-312431-de7zhswl 68 1 Retentates Retentates NNPS cord-312431-de7zhswl 68 2 were be VBD cord-312431-de7zhswl 68 3 then then RB cord-312431-de7zhswl 68 4 reconcentrated reconcentrate VBN cord-312431-de7zhswl 68 5 by by IN cord-312431-de7zhswl 68 6 ultracentrifugation ultracentrifugation NN cord-312431-de7zhswl 68 7 . . . cord-312431-de7zhswl 69 1 For for IN cord-312431-de7zhswl 69 2 ultracentrifugation ultracentrifugation NN cord-312431-de7zhswl 69 3 , , , cord-312431-de7zhswl 69 4 3 3 CD cord-312431-de7zhswl 69 5 runs run NNS cord-312431-de7zhswl 69 6 of of IN cord-312431-de7zhswl 69 7 3 3 CD cord-312431-de7zhswl 69 8 h h NN cord-312431-de7zhswl 69 9 30 30 CD cord-312431-de7zhswl 69 10 min min NN cord-312431-de7zhswl 69 11 each each DT cord-312431-de7zhswl 69 12 at at IN cord-312431-de7zhswl 69 13 130 130 CD cord-312431-de7zhswl 69 14 000 000 CD cord-312431-de7zhswl 69 15 Â â NN cord-312431-de7zhswl 69 16 g g NN cord-312431-de7zhswl 69 17 ( ( -LRB- cord-312431-de7zhswl 69 18 i.e. i.e. FW cord-312431-de7zhswl 69 19 29 29 CD cord-312431-de7zhswl 69 20 000 000 CD cord-312431-de7zhswl 69 21 rpm rpm NN cord-312431-de7zhswl 69 22 ) ) -RRB- cord-312431-de7zhswl 69 23 , , , cord-312431-de7zhswl 69 24 4 4 CD cord-312431-de7zhswl 69 25 ° ° NN cord-312431-de7zhswl 69 26 C c NN cord-312431-de7zhswl 69 27 with with IN cord-312431-de7zhswl 69 28 a a DT cord-312431-de7zhswl 69 29 SW-32 SW-32 NNP cord-312431-de7zhswl 69 30 Ti Ti NNP cord-312431-de7zhswl 69 31 rotor rotor NN cord-312431-de7zhswl 69 32 ( ( -LRB- cord-312431-de7zhswl 69 33 Optima Optima NNP cord-312431-de7zhswl 69 34 L-100 l-100 NN cord-312431-de7zhswl 69 35 XP XP NNP cord-312431-de7zhswl 69 36 , , , cord-312431-de7zhswl 69 37 Beckman Beckman NNP cord-312431-de7zhswl 69 38 Coulter Coulter NNP cord-312431-de7zhswl 69 39 ultracentrifuge ultracentrifuge NN cord-312431-de7zhswl 69 40 ) ) -RRB- cord-312431-de7zhswl 69 41 were be VBD cord-312431-de7zhswl 69 42 necessary necessary JJ cord-312431-de7zhswl 69 43 to to TO cord-312431-de7zhswl 69 44 ultracentrifuge ultracentrifuge VB cord-312431-de7zhswl 69 45 500 500 CD cord-312431-de7zhswl 69 46 mL ml NN cord-312431-de7zhswl 69 47 ( ( -LRB- cord-312431-de7zhswl 69 48 6 6 CD cord-312431-de7zhswl 69 49 tubes tube NNS cord-312431-de7zhswl 69 50 of of IN cord-312431-de7zhswl 69 51 30 30 CD cord-312431-de7zhswl 69 52 mL ml NN cord-312431-de7zhswl 69 53 filled fill VBN cord-312431-de7zhswl 69 54 with with IN cord-312431-de7zhswl 69 55 28 28 CD cord-312431-de7zhswl 69 56 mL mL NNS cord-312431-de7zhswl 69 57 of of IN cord-312431-de7zhswl 69 58 samples sample NNS cord-312431-de7zhswl 69 59 per per IN cord-312431-de7zhswl 69 60 run run NN cord-312431-de7zhswl 69 61 ) ) -RRB- cord-312431-de7zhswl 69 62 of of IN cord-312431-de7zhswl 69 63 the the DT cord-312431-de7zhswl 69 64 ultrafiltered ultrafiltered JJ cord-312431-de7zhswl 69 65 retentate retentate NN cord-312431-de7zhswl 69 66 [ [ -LRB- cord-312431-de7zhswl 69 67 38 38 CD cord-312431-de7zhswl 69 68 ] ] -RRB- cord-312431-de7zhswl 69 69 . . . cord-312431-de7zhswl 70 1 Viral viral JJ cord-312431-de7zhswl 70 2 pellets pellet NNS cord-312431-de7zhswl 70 3 were be VBD cord-312431-de7zhswl 70 4 then then RB cord-312431-de7zhswl 70 5 resuspended resuspend VBN cord-312431-de7zhswl 70 6 in in IN cord-312431-de7zhswl 70 7 5 5 CD cord-312431-de7zhswl 70 8 mL ml NN cord-312431-de7zhswl 70 9 of of IN cord-312431-de7zhswl 70 10 1 1 CD cord-312431-de7zhswl 70 11 Â â NN cord-312431-de7zhswl 70 12 phosphate phosphate NN cord-312431-de7zhswl 70 13 buffered buffer VBN cord-312431-de7zhswl 70 14 saline saline NN cord-312431-de7zhswl 70 15 ( ( -LRB- cord-312431-de7zhswl 70 16 pH pH NNP cord-312431-de7zhswl 70 17 7 7 CD cord-312431-de7zhswl 70 18 ) ) -RRB- cord-312431-de7zhswl 70 19 . . . cord-312431-de7zhswl 71 1 Normal normal JJ cord-312431-de7zhswl 71 2 water water NN cord-312431-de7zhswl 71 3 permeability permeability NN cord-312431-de7zhswl 71 4 ( ( -LRB- cord-312431-de7zhswl 71 5 NWP NWP NNP cord-312431-de7zhswl 71 6 ) ) -RRB- cord-312431-de7zhswl 71 7 testing testing NN cord-312431-de7zhswl 71 8 was be VBD cord-312431-de7zhswl 71 9 performed perform VBN cord-312431-de7zhswl 71 10 regularly regularly RB cord-312431-de7zhswl 71 11 on on IN cord-312431-de7zhswl 71 12 the the DT cord-312431-de7zhswl 71 13 TFF TFF NNP cord-312431-de7zhswl 71 14 filter filter NN cord-312431-de7zhswl 71 15 membranes membrane NNS cord-312431-de7zhswl 71 16 before before IN cord-312431-de7zhswl 71 17 and and CC cord-312431-de7zhswl 71 18 after after IN cord-312431-de7zhswl 71 19 running run VBG cord-312431-de7zhswl 71 20 the the DT cord-312431-de7zhswl 71 21 water water NN cord-312431-de7zhswl 71 22 samples sample NNS cord-312431-de7zhswl 71 23 through through IN cord-312431-de7zhswl 71 24 the the DT cord-312431-de7zhswl 71 25 system system NN cord-312431-de7zhswl 71 26 to to TO cord-312431-de7zhswl 71 27 ensure ensure VB cord-312431-de7zhswl 71 28 filter filter NN cord-312431-de7zhswl 71 29 quality quality NN cord-312431-de7zhswl 71 30 . . . cord-312431-de7zhswl 72 1 Proper proper JJ cord-312431-de7zhswl 72 2 maintenance maintenance NN cord-312431-de7zhswl 72 3 and and CC cord-312431-de7zhswl 72 4 preservation preservation NN cord-312431-de7zhswl 72 5 of of IN cord-312431-de7zhswl 72 6 filter filter NN cord-312431-de7zhswl 72 7 quality quality NN cord-312431-de7zhswl 72 8 was be VBD cord-312431-de7zhswl 72 9 carried carry VBN cord-312431-de7zhswl 72 10 out out RP cord-312431-de7zhswl 72 11 according accord VBG cord-312431-de7zhswl 72 12 to to IN cord-312431-de7zhswl 72 13 manufactures manufacture VBZ cord-312431-de7zhswl 72 14 instruction instruction NN cord-312431-de7zhswl 72 15 with with IN cord-312431-de7zhswl 72 16 some some DT cord-312431-de7zhswl 72 17 modifications modification NNS cord-312431-de7zhswl 72 18 . . . cord-312431-de7zhswl 73 1 NWP NWP NNP cord-312431-de7zhswl 73 2 was be VBD cord-312431-de7zhswl 73 3 calculated calculate VBN cord-312431-de7zhswl 73 4 using use VBG cord-312431-de7zhswl 73 5 the the DT cord-312431-de7zhswl 73 6 following follow VBG cord-312431-de7zhswl 73 7 equation equation NN cord-312431-de7zhswl 73 8 : : : cord-312431-de7zhswl 73 9 where where WRB cord-312431-de7zhswl 73 10 R r NN cord-312431-de7zhswl 73 11 is be VBZ cord-312431-de7zhswl 73 12 the the DT cord-312431-de7zhswl 73 13 permeate permeate JJ cord-312431-de7zhswl 73 14 flow flow NN cord-312431-de7zhswl 73 15 rate rate NN cord-312431-de7zhswl 73 16 ( ( -LRB- cord-312431-de7zhswl 73 17 L l NN cord-312431-de7zhswl 73 18 / / SYM cord-312431-de7zhswl 73 19 h h NNP cord-312431-de7zhswl 73 20 ) ) -RRB- cord-312431-de7zhswl 73 21 , , , cord-312431-de7zhswl 73 22 P p NN cord-312431-de7zhswl 73 23 in in IN cord-312431-de7zhswl 73 24 the the DT cord-312431-de7zhswl 73 25 feed feed NN cord-312431-de7zhswl 73 26 inlet inlet NN cord-312431-de7zhswl 73 27 pressure pressure NN cord-312431-de7zhswl 73 28 ( ( -LRB- cord-312431-de7zhswl 73 29 psi psi NNP cord-312431-de7zhswl 73 30 ) ) -RRB- cord-312431-de7zhswl 73 31 , , , cord-312431-de7zhswl 73 32 P p NN cord-312431-de7zhswl 73 33 out out RP cord-312431-de7zhswl 73 34 the the DT cord-312431-de7zhswl 73 35 retentate retentate NN cord-312431-de7zhswl 73 36 discharge discharge NN cord-312431-de7zhswl 73 37 pressure pressure NN cord-312431-de7zhswl 73 38 ( ( -LRB- cord-312431-de7zhswl 73 39 psi psi NNP cord-312431-de7zhswl 73 40 ) ) -RRB- cord-312431-de7zhswl 73 41 , , , cord-312431-de7zhswl 73 42 P p NN cord-312431-de7zhswl 73 43 p p NN cord-312431-de7zhswl 73 44 the the DT cord-312431-de7zhswl 73 45 permeate permeate NN cord-312431-de7zhswl 73 46 discharge discharge NN cord-312431-de7zhswl 73 47 pressure pressure NN cord-312431-de7zhswl 73 48 ( ( -LRB- cord-312431-de7zhswl 73 49 psi psi NNP cord-312431-de7zhswl 73 50 ) ) -RRB- cord-312431-de7zhswl 73 51 , , , cord-312431-de7zhswl 73 52 A a DT cord-312431-de7zhswl 73 53 the the DT cord-312431-de7zhswl 73 54 total total JJ cord-312431-de7zhswl 73 55 filter filter NN cord-312431-de7zhswl 73 56 area area NN cord-312431-de7zhswl 73 57 ( ( -LRB- cord-312431-de7zhswl 73 58 m m NNP cord-312431-de7zhswl 73 59 2 2 CD cord-312431-de7zhswl 73 60 ) ) -RRB- cord-312431-de7zhswl 73 61 and and CC cord-312431-de7zhswl 74 1 F F NNP cord-312431-de7zhswl 74 2 is be VBZ cord-312431-de7zhswl 74 3 the the DT cord-312431-de7zhswl 74 4 temperature temperature NN cord-312431-de7zhswl 74 5 correction correction NN cord-312431-de7zhswl 74 6 factor factor NN cord-312431-de7zhswl 74 7 ( ( -LRB- cord-312431-de7zhswl 74 8 based base VBN cord-312431-de7zhswl 74 9 on on IN cord-312431-de7zhswl 74 10 water water NN cord-312431-de7zhswl 74 11 fluidity fluidity NN cord-312431-de7zhswl 74 12 relative relative JJ cord-312431-de7zhswl 74 13 to to IN cord-312431-de7zhswl 74 14 25 25 CD cord-312431-de7zhswl 74 15 ° ° NN cord-312431-de7zhswl 74 16 C c NN cord-312431-de7zhswl 74 17 ) ) -RRB- cord-312431-de7zhswl 74 18 . . . cord-312431-de7zhswl 75 1 SYBR SYBR NNP cord-312431-de7zhswl 75 2 gold gold NN cord-312431-de7zhswl 75 3 staining staining NN cord-312431-de7zhswl 75 4 coupled couple VBN cord-312431-de7zhswl 75 5 with with IN cord-312431-de7zhswl 75 6 epifluorescent epifluorescent NN cord-312431-de7zhswl 75 7 microscopy microscopy NNS cord-312431-de7zhswl 75 8 [ [ -LRB- cord-312431-de7zhswl 75 9 39 39 CD cord-312431-de7zhswl 75 10 ] ] -RRB- cord-312431-de7zhswl 76 1 [ [ -LRB- cord-312431-de7zhswl 76 2 40 40 CD cord-312431-de7zhswl 76 3 ] ] -RRB- cord-312431-de7zhswl 77 1 [ [ -LRB- cord-312431-de7zhswl 77 2 41 41 CD cord-312431-de7zhswl 77 3 ] ] -RRB- cord-312431-de7zhswl 77 4 was be VBD cord-312431-de7zhswl 77 5 used use VBN cord-312431-de7zhswl 77 6 to to TO cord-312431-de7zhswl 77 7 enumerate enumerate VB cord-312431-de7zhswl 77 8 VLPs vlp NNS cord-312431-de7zhswl 77 9 from from IN cord-312431-de7zhswl 77 10 the the DT cord-312431-de7zhswl 77 11 Umgeni Umgeni NNP cord-312431-de7zhswl 77 12 River River NNP cord-312431-de7zhswl 77 13 virus virus NN cord-312431-de7zhswl 77 14 filtrate filtrate NN cord-312431-de7zhswl 77 15 . . . cord-312431-de7zhswl 78 1 After after IN cord-312431-de7zhswl 78 2 UF UF NNP cord-312431-de7zhswl 78 3 one one CD cord-312431-de7zhswl 78 4 millilitre millilitre NN cord-312431-de7zhswl 78 5 ( ( -LRB- cord-312431-de7zhswl 78 6 100 100 CD cord-312431-de7zhswl 78 7 kDa kDa NNPS cord-312431-de7zhswl 78 8 cut cut VBN cord-312431-de7zhswl 78 9 - - HYPH cord-312431-de7zhswl 78 10 off off RP cord-312431-de7zhswl 78 11 ) ) -RRB- cord-312431-de7zhswl 78 12 of of IN cord-312431-de7zhswl 78 13 the the DT cord-312431-de7zhswl 78 14 concentrated concentrated JJ cord-312431-de7zhswl 78 15 water water NN cord-312431-de7zhswl 78 16 samples sample NNS cord-312431-de7zhswl 78 17 were be VBD cord-312431-de7zhswl 78 18 fixed fix VBN cord-312431-de7zhswl 78 19 with with IN cord-312431-de7zhswl 78 20 40 40 CD cord-312431-de7zhswl 78 21 mL mL NNS cord-312431-de7zhswl 78 22 of of IN cord-312431-de7zhswl 78 23 a a DT cord-312431-de7zhswl 78 24 2 2 CD cord-312431-de7zhswl 78 25 % % NN cord-312431-de7zhswl 78 26 paraformaldehyde paraformaldehyde JJ cord-312431-de7zhswl 78 27 solution solution NN cord-312431-de7zhswl 78 28 and and CC cord-312431-de7zhswl 78 29 then then RB cord-312431-de7zhswl 78 30 filtered filter VBN cord-312431-de7zhswl 78 31 through through IN cord-312431-de7zhswl 78 32 0.02 0.02 CD cord-312431-de7zhswl 78 33 mm mm NNP cord-312431-de7zhswl 78 34 Anodisc Anodisc NNP cord-312431-de7zhswl 78 35 filters filter NNS cord-312431-de7zhswl 78 36 ( ( -LRB- cord-312431-de7zhswl 78 37 Whatman Whatman NNP cord-312431-de7zhswl 78 38 , , , cord-312431-de7zhswl 78 39 Maidstone Maidstone NNP cord-312431-de7zhswl 78 40 , , , cord-312431-de7zhswl 78 41 Kent Kent NNP cord-312431-de7zhswl 78 42 , , , cord-312431-de7zhswl 78 43 UK UK NNP cord-312431-de7zhswl 78 44 ) ) -RRB- cord-312431-de7zhswl 78 45 with with IN cord-312431-de7zhswl 78 46 vacuum vacuum NN cord-312431-de7zhswl 78 47 pressure pressure NN cord-312431-de7zhswl 78 48 20 20 CD cord-312431-de7zhswl 78 49 kPa kPa NNS cord-312431-de7zhswl 78 50 . . . cord-312431-de7zhswl 79 1 The the DT cord-312431-de7zhswl 79 2 Anodisc Anodisc NNP cord-312431-de7zhswl 79 3 filters filter NNS cord-312431-de7zhswl 79 4 were be VBD cord-312431-de7zhswl 79 5 allowed allow VBN cord-312431-de7zhswl 79 6 to to TO cord-312431-de7zhswl 79 7 air air NN cord-312431-de7zhswl 79 8 dry dry JJ cord-312431-de7zhswl 79 9 and and CC cord-312431-de7zhswl 79 10 were be VBD cord-312431-de7zhswl 79 11 then then RB cord-312431-de7zhswl 79 12 stained stain VBN cord-312431-de7zhswl 79 13 with with IN cord-312431-de7zhswl 79 14 2 2 CD cord-312431-de7zhswl 79 15 Â â NN cord-312431-de7zhswl 79 16 SYBR SYBR NNP cord-312431-de7zhswl 79 17 Gold Gold NNP cord-312431-de7zhswl 79 18 ( ( -LRB- cord-312431-de7zhswl 79 19 Invitrogen Invitrogen NNP cord-312431-de7zhswl 79 20 , , , cord-312431-de7zhswl 79 21 Carlsbad Carlsbad NNP cord-312431-de7zhswl 79 22 , , , cord-312431-de7zhswl 79 23 CA CA NNP cord-312431-de7zhswl 79 24 , , , cord-312431-de7zhswl 79 25 USA USA NNP cord-312431-de7zhswl 79 26 ) ) -RRB- cord-312431-de7zhswl 79 27 for for IN cord-312431-de7zhswl 79 28 15 15 CD cord-312431-de7zhswl 79 29 min min NN cord-312431-de7zhswl 79 30 in in IN cord-312431-de7zhswl 79 31 the the DT cord-312431-de7zhswl 79 32 dark dark NN cord-312431-de7zhswl 79 33 . . . cord-312431-de7zhswl 80 1 After after IN cord-312431-de7zhswl 80 2 staining stain VBG cord-312431-de7zhswl 80 3 the the DT cord-312431-de7zhswl 80 4 filters filter NNS cord-312431-de7zhswl 80 5 were be VBD cord-312431-de7zhswl 80 6 wicked wicked JJ cord-312431-de7zhswl 80 7 to to TO cord-312431-de7zhswl 80 8 remove remove VB cord-312431-de7zhswl 80 9 any any DT cord-312431-de7zhswl 80 10 remaining remain VBG cord-312431-de7zhswl 80 11 solution solution NN cord-312431-de7zhswl 80 12 and and CC cord-312431-de7zhswl 80 13 air air NN cord-312431-de7zhswl 80 14 dried dry VBN cord-312431-de7zhswl 80 15 . . . cord-312431-de7zhswl 81 1 The the DT cord-312431-de7zhswl 81 2 filters filter NNS cord-312431-de7zhswl 81 3 were be VBD cord-312431-de7zhswl 81 4 mounted mount VBN cord-312431-de7zhswl 81 5 onto onto IN cord-312431-de7zhswl 81 6 glass glass NN cord-312431-de7zhswl 81 7 slides slide NNS cord-312431-de7zhswl 81 8 , , , cord-312431-de7zhswl 81 9 and and CC cord-312431-de7zhswl 81 10 counted count VBD cord-312431-de7zhswl 81 11 digitally digitally RB cord-312431-de7zhswl 81 12 at at IN cord-312431-de7zhswl 81 13 1000Â 1000â CD cord-312431-de7zhswl 81 14 magnification magnification NN cord-312431-de7zhswl 81 15 under under IN cord-312431-de7zhswl 81 16 blue blue JJ cord-312431-de7zhswl 81 17 - - HYPH cord-312431-de7zhswl 81 18 green green JJ cord-312431-de7zhswl 81 19 light light JJ cord-312431-de7zhswl 81 20 excitation excitation NN cord-312431-de7zhswl 81 21 ( ( -LRB- cord-312431-de7zhswl 81 22 Fitch Fitch NNP cord-312431-de7zhswl 81 23 filter filter NN cord-312431-de7zhswl 81 24 , , , cord-312431-de7zhswl 81 25 excitation excitation NN cord-312431-de7zhswl 81 26 at at IN cord-312431-de7zhswl 81 27 480 480 CD cord-312431-de7zhswl 81 28 - - SYM cord-312431-de7zhswl 81 29 495 495 CD cord-312431-de7zhswl 81 30 nm nm CD cord-312431-de7zhswl 81 31 ) ) -RRB- cord-312431-de7zhswl 81 32 with with IN cord-312431-de7zhswl 81 33 a a DT cord-312431-de7zhswl 81 34 NIKON NIKON NNP cord-312431-de7zhswl 81 35 Eclipse Eclipse NNP cord-312431-de7zhswl 81 36 ( ( -LRB- cord-312431-de7zhswl 81 37 80i 80i NNS cord-312431-de7zhswl 81 38 ) ) -RRB- cord-312431-de7zhswl 81 39 epifluorescent epifluorescent NN cord-312431-de7zhswl 81 40 microscope microscope NN cord-312431-de7zhswl 81 41 in in IN cord-312431-de7zhswl 81 42 at at RB cord-312431-de7zhswl 81 43 least least RBS cord-312431-de7zhswl 81 44 eight eight CD cord-312431-de7zhswl 81 45 fields field NNS cord-312431-de7zhswl 81 46 of of IN cord-312431-de7zhswl 81 47 view view NN cord-312431-de7zhswl 81 48 for for IN cord-312431-de7zhswl 81 49 each each DT cord-312431-de7zhswl 81 50 sample sample NN cord-312431-de7zhswl 81 51 [ [ -LRB- cord-312431-de7zhswl 81 52 39 39 CD cord-312431-de7zhswl 81 53 ] ] -RRB- cord-312431-de7zhswl 81 54 . . . cord-312431-de7zhswl 82 1 Images image NNS cord-312431-de7zhswl 82 2 obtained obtain VBN cord-312431-de7zhswl 82 3 were be VBD cord-312431-de7zhswl 82 4 then then RB cord-312431-de7zhswl 82 5 analysed analyse VBN cord-312431-de7zhswl 82 6 using use VBG cord-312431-de7zhswl 82 7 the the DT cord-312431-de7zhswl 82 8 iTEM item JJ cord-312431-de7zhswl 82 9 software software NN cord-312431-de7zhswl 82 10 and and CC cord-312431-de7zhswl 82 11 NIS NIS NNP cord-312431-de7zhswl 82 12 - - HYPH cord-312431-de7zhswl 82 13 D D NNP cord-312431-de7zhswl 82 14 Elements Elements NNP cord-312431-de7zhswl 82 15 software software NN cord-312431-de7zhswl 82 16 ( ( -LRB- cord-312431-de7zhswl 82 17 D D NNP cord-312431-de7zhswl 82 18 3.2 3.2 CD cord-312431-de7zhswl 82 19 ) ) -RRB- cord-312431-de7zhswl 82 20 to to TO cord-312431-de7zhswl 82 21 digitally digitally RB cord-312431-de7zhswl 82 22 count count VB cord-312431-de7zhswl 82 23 the the DT cord-312431-de7zhswl 82 24 fluorescent fluorescent JJ cord-312431-de7zhswl 82 25 green green JJ cord-312431-de7zhswl 82 26 VLP VLP NNP cord-312431-de7zhswl 82 27 spots spot NNS cord-312431-de7zhswl 82 28 and and CC cord-312431-de7zhswl 82 29 compute compute VB cord-312431-de7zhswl 82 30 the the DT cord-312431-de7zhswl 82 31 results result NNS cord-312431-de7zhswl 82 32 . . . cord-312431-de7zhswl 83 1 TEM TEM NNP cord-312431-de7zhswl 83 2 was be VBD cord-312431-de7zhswl 83 3 used use VBN cord-312431-de7zhswl 83 4 to to TO cord-312431-de7zhswl 83 5 examine examine VB cord-312431-de7zhswl 83 6 the the DT cord-312431-de7zhswl 83 7 structures structure NNS cord-312431-de7zhswl 83 8 and and CC cord-312431-de7zhswl 83 9 morphology morphology NN cord-312431-de7zhswl 83 10 of of IN cord-312431-de7zhswl 83 11 VLPs vlp NNS cord-312431-de7zhswl 83 12 in in IN cord-312431-de7zhswl 83 13 the the DT cord-312431-de7zhswl 83 14 river river NN cord-312431-de7zhswl 83 15 water water NN cord-312431-de7zhswl 83 16 . . . cord-312431-de7zhswl 84 1 These these DT cord-312431-de7zhswl 84 2 TEM tem NN cord-312431-de7zhswl 84 3 images image NNS cord-312431-de7zhswl 84 4 were be VBD cord-312431-de7zhswl 84 5 then then RB cord-312431-de7zhswl 84 6 compared compare VBN cord-312431-de7zhswl 84 7 to to IN cord-312431-de7zhswl 84 8 known know VBN cord-312431-de7zhswl 84 9 viral viral JJ cord-312431-de7zhswl 84 10 images image NNS cord-312431-de7zhswl 84 11 of of IN cord-312431-de7zhswl 84 12 human human JJ cord-312431-de7zhswl 84 13 origin origin NN cord-312431-de7zhswl 84 14 where where WRB cord-312431-de7zhswl 84 15 possible possible JJ cord-312431-de7zhswl 84 16 [ [ -LRB- cord-312431-de7zhswl 84 17 42 42 CD cord-312431-de7zhswl 84 18 ] ] -RRB- cord-312431-de7zhswl 84 19 . . . cord-312431-de7zhswl 85 1 Briefly briefly RB cord-312431-de7zhswl 85 2 one one CD cord-312431-de7zhswl 85 3 drop drop NN cord-312431-de7zhswl 85 4 of of IN cord-312431-de7zhswl 85 5 freshly freshly RB cord-312431-de7zhswl 85 6 prepared prepare VBN cord-312431-de7zhswl 85 7 VLPs vlp NNS cord-312431-de7zhswl 85 8 was be VBD cord-312431-de7zhswl 85 9 spotted spot VBN cord-312431-de7zhswl 85 10 onto onto IN cord-312431-de7zhswl 85 11 a a DT cord-312431-de7zhswl 85 12 Formvar Formvar NNP cord-312431-de7zhswl 85 13 - - HYPH cord-312431-de7zhswl 85 14 carbon carbon NN cord-312431-de7zhswl 85 15 - - HYPH cord-312431-de7zhswl 85 16 coated coat VBN cord-312431-de7zhswl 85 17 200mesh 200mesh JJ cord-312431-de7zhswl 85 18 TEM tem NN cord-312431-de7zhswl 85 19 grid grid NN cord-312431-de7zhswl 85 20 ( ( -LRB- cord-312431-de7zhswl 85 21 Electron Electron NNP cord-312431-de7zhswl 85 22 Microscopy Microscopy NNP cord-312431-de7zhswl 85 23 Sciences Sciences NNPS cord-312431-de7zhswl 85 24 , , , cord-312431-de7zhswl 85 25 Fort Fort NNP cord-312431-de7zhswl 85 26 Washington Washington NNP cord-312431-de7zhswl 85 27 , , , cord-312431-de7zhswl 85 28 PA PA NNP cord-312431-de7zhswl 85 29 ) ) -RRB- cord-312431-de7zhswl 85 30 . . . cord-312431-de7zhswl 86 1 The the DT cord-312431-de7zhswl 86 2 edge edge NN cord-312431-de7zhswl 86 3 of of IN cord-312431-de7zhswl 86 4 the the DT cord-312431-de7zhswl 86 5 grid grid NN cord-312431-de7zhswl 86 6 was be VBD cord-312431-de7zhswl 86 7 gently gently RB cord-312431-de7zhswl 86 8 blotted blot VBN cord-312431-de7zhswl 86 9 with with IN cord-312431-de7zhswl 86 10 a a DT cord-312431-de7zhswl 86 11 piece piece NN cord-312431-de7zhswl 86 12 of of IN cord-312431-de7zhswl 86 13 Whatman Whatman NNP cord-312431-de7zhswl 86 14 filter filter NN cord-312431-de7zhswl 86 15 paper paper NN cord-312431-de7zhswl 86 16 to to TO cord-312431-de7zhswl 86 17 drain drain VB cord-312431-de7zhswl 86 18 away away RB cord-312431-de7zhswl 86 19 the the DT cord-312431-de7zhswl 86 20 excess excess JJ cord-312431-de7zhswl 86 21 fluid fluid NN cord-312431-de7zhswl 86 22 , , , cord-312431-de7zhswl 86 23 and and CC cord-312431-de7zhswl 86 24 the the DT cord-312431-de7zhswl 86 25 grid grid NN cord-312431-de7zhswl 86 26 was be VBD cord-312431-de7zhswl 86 27 then then RB cord-312431-de7zhswl 86 28 stained stain VBN cord-312431-de7zhswl 86 29 with with IN cord-312431-de7zhswl 86 30 a a DT cord-312431-de7zhswl 86 31 1 1 CD cord-312431-de7zhswl 86 32 % % NN cord-312431-de7zhswl 86 33 phospho phospho JJ cord-312431-de7zhswl 86 34 - - HYPH cord-312431-de7zhswl 86 35 tungstic tungstic JJ cord-312431-de7zhswl 86 36 acid acid NN cord-312431-de7zhswl 86 37 ( ( -LRB- cord-312431-de7zhswl 86 38 PTA PTA NNP cord-312431-de7zhswl 86 39 ) ) -RRB- cord-312431-de7zhswl 86 40 solution solution NN cord-312431-de7zhswl 86 41 or or CC cord-312431-de7zhswl 86 42 a a DT cord-312431-de7zhswl 86 43 2 2 CD cord-312431-de7zhswl 86 44 % % NN cord-312431-de7zhswl 86 45 uranyl uranyl NN cord-312431-de7zhswl 86 46 acetate acetate NN cord-312431-de7zhswl 86 47 solution solution NN cord-312431-de7zhswl 86 48 for for IN cord-312431-de7zhswl 86 49 30 30 CD cord-312431-de7zhswl 86 50 s s NNS cord-312431-de7zhswl 86 51 , , , cord-312431-de7zhswl 86 52 washed wash VBN cord-312431-de7zhswl 86 53 with with IN cord-312431-de7zhswl 86 54 1 1 CD cord-312431-de7zhswl 86 55 drop drop NN cord-312431-de7zhswl 86 56 of of IN cord-312431-de7zhswl 86 57 deionised deionised JJ cord-312431-de7zhswl 86 58 water water NN cord-312431-de7zhswl 86 59 for for IN cord-312431-de7zhswl 86 60 10 10 CD cord-312431-de7zhswl 86 61 s s NNS cord-312431-de7zhswl 86 62 , , , cord-312431-de7zhswl 86 63 and and CC cord-312431-de7zhswl 86 64 air air NN cord-312431-de7zhswl 86 65 - - HYPH cord-312431-de7zhswl 86 66 dried dry VBN cord-312431-de7zhswl 86 67 before before IN cord-312431-de7zhswl 86 68 examination examination NN cord-312431-de7zhswl 86 69 with with IN cord-312431-de7zhswl 86 70 a a DT cord-312431-de7zhswl 86 71 TEM tem NN cord-312431-de7zhswl 86 72 ( ( -LRB- cord-312431-de7zhswl 86 73 JEOL JEOL NNP cord-312431-de7zhswl 86 74 ) ) -RRB- cord-312431-de7zhswl 86 75 . . . cord-312431-de7zhswl 87 1 Photomicrographs photomicrographs NN cord-312431-de7zhswl 87 2 of of IN cord-312431-de7zhswl 87 3 viruses virus NNS cord-312431-de7zhswl 87 4 were be VBD cord-312431-de7zhswl 87 5 taken take VBN cord-312431-de7zhswl 87 6 at at IN cord-312431-de7zhswl 87 7 magnifications magnification NNS cord-312431-de7zhswl 87 8 of of IN cord-312431-de7zhswl 87 9 150 150 CD cord-312431-de7zhswl 87 10 000Â 000â CD cord-312431-de7zhswl 87 11 to to IN cord-312431-de7zhswl 87 12 600 600 CD cord-312431-de7zhswl 87 13 000Â. 000â. CD cord-312431-de7zhswl 88 1 Morphological morphological JJ cord-312431-de7zhswl 88 2 characteristics characteristic NNS cord-312431-de7zhswl 88 3 of of IN cord-312431-de7zhswl 88 4 VLPs vlp NNS cord-312431-de7zhswl 88 5 were be VBD cord-312431-de7zhswl 88 6 compiled compile VBN cord-312431-de7zhswl 88 7 from from IN cord-312431-de7zhswl 88 8 multiple multiple JJ cord-312431-de7zhswl 88 9 photomicrographs photomicrograph NNS cord-312431-de7zhswl 88 10 of of IN cord-312431-de7zhswl 88 11 phage phage NN cord-312431-de7zhswl 88 12 particles particle NNS cord-312431-de7zhswl 88 13 in in IN cord-312431-de7zhswl 88 14 order order NN cord-312431-de7zhswl 88 15 to to TO cord-312431-de7zhswl 88 16 minimise minimise VB cord-312431-de7zhswl 88 17 size size NN cord-312431-de7zhswl 88 18 or or CC cord-312431-de7zhswl 88 19 shape shape NN cord-312431-de7zhswl 88 20 anomalies anomaly NNS cord-312431-de7zhswl 88 21 . . . cord-312431-de7zhswl 89 1 Cell cell NN cord-312431-de7zhswl 89 2 - - HYPH cord-312431-de7zhswl 89 3 culture culture NN cord-312431-de7zhswl 89 4 ( ( -LRB- cord-312431-de7zhswl 89 5 where where WRB cord-312431-de7zhswl 89 6 cell cell NN cord-312431-de7zhswl 89 7 lines line NNS cord-312431-de7zhswl 89 8 were be VBD cord-312431-de7zhswl 89 9 available available JJ cord-312431-de7zhswl 89 10 ) ) -RRB- cord-312431-de7zhswl 89 11 was be VBD cord-312431-de7zhswl 89 12 used use VBN cord-312431-de7zhswl 89 13 to to TO cord-312431-de7zhswl 89 14 determine determine VB cord-312431-de7zhswl 89 15 infectivity infectivity NN cord-312431-de7zhswl 89 16 of of IN cord-312431-de7zhswl 89 17 VLPs vlp NNS cord-312431-de7zhswl 89 18 . . . cord-312431-de7zhswl 90 1 The the DT cord-312431-de7zhswl 90 2 total total JJ cord-312431-de7zhswl 90 3 cultivable cultivable JJ cord-312431-de7zhswl 90 4 virus virus NN cord-312431-de7zhswl 90 5 method method NN cord-312431-de7zhswl 90 6 as as IN cord-312431-de7zhswl 90 7 described describe VBN cord-312431-de7zhswl 90 8 in in IN cord-312431-de7zhswl 90 9 US US NNP cord-312431-de7zhswl 90 10 EPA EPA NNP cord-312431-de7zhswl 90 11 [ [ -LRB- cord-312431-de7zhswl 90 12 43 43 CD cord-312431-de7zhswl 90 13 ] ] -RRB- cord-312431-de7zhswl 90 14 was be VBD cord-312431-de7zhswl 90 15 used use VBN cord-312431-de7zhswl 90 16 as as IN cord-312431-de7zhswl 90 17 the the DT cord-312431-de7zhswl 90 18 infectivity infectivity NN cord-312431-de7zhswl 90 19 protocol protocol NN cord-312431-de7zhswl 90 20 . . . cord-312431-de7zhswl 91 1 The the DT cord-312431-de7zhswl 91 2 concentrated concentrated JJ cord-312431-de7zhswl 91 3 VLPs vlp NNS cord-312431-de7zhswl 91 4 from from IN cord-312431-de7zhswl 91 5 various various JJ cord-312431-de7zhswl 91 6 water water NN cord-312431-de7zhswl 91 7 sources source NNS cord-312431-de7zhswl 91 8 were be VBD cord-312431-de7zhswl 91 9 fed feed VBN cord-312431-de7zhswl 91 10 into into IN cord-312431-de7zhswl 91 11 the the DT cord-312431-de7zhswl 91 12 various various JJ cord-312431-de7zhswl 91 13 cell cell NN cord-312431-de7zhswl 91 14 lines line NNS cord-312431-de7zhswl 91 15 . . . cord-312431-de7zhswl 92 1 Three three CD cord-312431-de7zhswl 92 2 known know VBN cord-312431-de7zhswl 92 3 viruses virus NNS cord-312431-de7zhswl 92 4 : : : cord-312431-de7zhswl 92 5 human human JJ cord-312431-de7zhswl 92 6 adenovirus adenovirus NN cord-312431-de7zhswl 92 7 , , , cord-312431-de7zhswl 92 8 group group NN cord-312431-de7zhswl 92 9 A a DT cord-312431-de7zhswl 92 10 rotavirus rotavirus NN cord-312431-de7zhswl 92 11 and and CC cord-312431-de7zhswl 92 12 coxsackie coxsackie NNP cord-312431-de7zhswl 92 13 B B NNP cord-312431-de7zhswl 92 14 virus virus NN cord-312431-de7zhswl 92 15 were be VBD cord-312431-de7zhswl 92 16 used use VBN cord-312431-de7zhswl 92 17 as as IN cord-312431-de7zhswl 92 18 positive positive JJ cord-312431-de7zhswl 92 19 controls control NNS cord-312431-de7zhswl 92 20 . . . cord-312431-de7zhswl 93 1 Amongst amongst IN cord-312431-de7zhswl 93 2 the the DT cord-312431-de7zhswl 93 3 cell cell NN cord-312431-de7zhswl 93 4 lines line NNS cord-312431-de7zhswl 93 5 used use VBN cord-312431-de7zhswl 93 6 for for IN cord-312431-de7zhswl 93 7 human human JJ cord-312431-de7zhswl 93 8 virus virus NN cord-312431-de7zhswl 93 9 investigations investigation NNS cord-312431-de7zhswl 93 10 in in IN cord-312431-de7zhswl 93 11 water water NN cord-312431-de7zhswl 93 12 were be VBD cord-312431-de7zhswl 93 13 , , , cord-312431-de7zhswl 93 14 the the DT cord-312431-de7zhswl 93 15 A549 A549 NNP cord-312431-de7zhswl 93 16 ( ( -LRB- cord-312431-de7zhswl 93 17 adenocarcinomic adenocarcinomic NNP cord-312431-de7zhswl 93 18 human human JJ cord-312431-de7zhswl 93 19 alveolar alveolar NN cord-312431-de7zhswl 93 20 basal basal NN cord-312431-de7zhswl 93 21 epithelial epithelial JJ cord-312431-de7zhswl 93 22 cell cell NN cord-312431-de7zhswl 93 23 ) ) -RRB- cord-312431-de7zhswl 93 24 , , . cord-312431-de7zhswl 94 1 HEK293 HEK293 NNP cord-312431-de7zhswl 94 2 ( ( -LRB- cord-312431-de7zhswl 94 3 human human JJ cord-312431-de7zhswl 94 4 embryonic embryonic JJ cord-312431-de7zhswl 94 5 kidney kidney NN cord-312431-de7zhswl 94 6 cell cell NN cord-312431-de7zhswl 94 7 ) ) -RRB- cord-312431-de7zhswl 95 1 , , , cord-312431-de7zhswl 95 2 Hela Hela NNP cord-312431-de7zhswl 95 3 ( ( -LRB- cord-312431-de7zhswl 95 4 humancervical humancervical JJ cord-312431-de7zhswl 95 5 cancer cancer NN cord-312431-de7zhswl 95 6 cell cell NN cord-312431-de7zhswl 95 7 ) ) -RRB- cord-312431-de7zhswl 95 8 , , , cord-312431-de7zhswl 95 9 HepG2 HepG2 NNP cord-312431-de7zhswl 95 10 ( ( -LRB- cord-312431-de7zhswl 95 11 human human JJ cord-312431-de7zhswl 95 12 hepatocellular hepatocellular JJ cord-312431-de7zhswl 95 13 carcinoma carcinoma NN cord-312431-de7zhswl 95 14 cell cell NN cord-312431-de7zhswl 95 15 ) ) -RRB- cord-312431-de7zhswl 96 1 , , , cord-312431-de7zhswl 96 2 PLC PLC NNP cord-312431-de7zhswl 96 3 / / SYM cord-312431-de7zhswl 96 4 PRF/5 PRF/5 NNP cord-312431-de7zhswl 96 5 ( ( -LRB- cord-312431-de7zhswl 96 6 human human JJ cord-312431-de7zhswl 96 7 primary primary JJ cord-312431-de7zhswl 96 8 liver liver NN cord-312431-de7zhswl 96 9 carcinoma carcinoma NN cord-312431-de7zhswl 96 10 cell cell NN cord-312431-de7zhswl 96 11 ) ) -RRB- cord-312431-de7zhswl 96 12 and and CC cord-312431-de7zhswl 96 13 Vero Vero NNP cord-312431-de7zhswl 96 14 ( ( -LRB- cord-312431-de7zhswl 96 15 African african JJ cord-312431-de7zhswl 96 16 green green JJ cord-312431-de7zhswl 96 17 monkey monkey NN cord-312431-de7zhswl 96 18 kidney kidney NN cord-312431-de7zhswl 96 19 cell cell NN cord-312431-de7zhswl 96 20 ) ) -RRB- cord-312431-de7zhswl 96 21 . . . cord-312431-de7zhswl 97 1 Cells cell NNS cord-312431-de7zhswl 97 2 were be VBD cord-312431-de7zhswl 97 3 grown grow VBN cord-312431-de7zhswl 97 4 in in IN cord-312431-de7zhswl 97 5 10 10 CD cord-312431-de7zhswl 97 6 % % NN cord-312431-de7zhswl 97 7 Dulbecco Dulbecco NNP cord-312431-de7zhswl 97 8 's 's POS cord-312431-de7zhswl 97 9 modified modify VBN cord-312431-de7zhswl 97 10 essential essential JJ cord-312431-de7zhswl 97 11 medium medium NN cord-312431-de7zhswl 97 12 ( ( -LRB- cord-312431-de7zhswl 97 13 GIBCO GIBCO NNP cord-312431-de7zhswl 97 14 ) ) -RRB- cord-312431-de7zhswl 97 15 supplemented supplement VBD cord-312431-de7zhswl 97 16 with with IN cord-312431-de7zhswl 97 17 10 10 CD cord-312431-de7zhswl 97 18 % % NN cord-312431-de7zhswl 97 19 foetal foetal JJ cord-312431-de7zhswl 97 20 calf calf NN cord-312431-de7zhswl 97 21 serum serum NN cord-312431-de7zhswl 97 22 ( ( -LRB- cord-312431-de7zhswl 97 23 GIBCO GIBCO NNP cord-312431-de7zhswl 97 24 ) ) -RRB- cord-312431-de7zhswl 97 25 containing contain VBG cord-312431-de7zhswl 97 26 a a DT cord-312431-de7zhswl 97 27 penicillin penicillin NN cord-312431-de7zhswl 97 28 / / SYM cord-312431-de7zhswl 97 29 streptomycin streptomycin NN cord-312431-de7zhswl 97 30 / / SYM cord-312431-de7zhswl 97 31 fungizone fungizone NN cord-312431-de7zhswl 97 32 mix mix NN cord-312431-de7zhswl 97 33 ( ( -LRB- cord-312431-de7zhswl 97 34 1:1:1 1:1:1 CD cord-312431-de7zhswl 97 35 ) ) -RRB- cord-312431-de7zhswl 97 36 by by IN cord-312431-de7zhswl 97 37 volume volume NN cord-312431-de7zhswl 97 38 , , , cord-312431-de7zhswl 97 39 to to IN cord-312431-de7zhswl 97 40 confluent confluent JJ cord-312431-de7zhswl 97 41 monolayers monolayer NNS cord-312431-de7zhswl 97 42 in in IN cord-312431-de7zhswl 97 43 24 24 CD cord-312431-de7zhswl 97 44 well well JJ cord-312431-de7zhswl 97 45 tissue tissue NN cord-312431-de7zhswl 97 46 culture culture NN cord-312431-de7zhswl 97 47 plates plate NNS cord-312431-de7zhswl 97 48 ( ( -LRB- cord-312431-de7zhswl 97 49 Corning Corning NNP cord-312431-de7zhswl 97 50 , , , cord-312431-de7zhswl 97 51 USA USA NNP cord-312431-de7zhswl 97 52 ) ) -RRB- cord-312431-de7zhswl 97 53 . . . cord-312431-de7zhswl 98 1 Approximately approximately RB cord-312431-de7zhswl 98 2 200 200 CD cord-312431-de7zhswl 98 3 mL ml NN cord-312431-de7zhswl 98 4 of of IN cord-312431-de7zhswl 98 5 viral viral JJ cord-312431-de7zhswl 98 6 concentrate concentrate NN cord-312431-de7zhswl 98 7 was be VBD cord-312431-de7zhswl 98 8 overlaid overlay VBN cord-312431-de7zhswl 98 9 onto onto IN cord-312431-de7zhswl 98 10 the the DT cord-312431-de7zhswl 98 11 monolayers monolayer NNS cord-312431-de7zhswl 98 12 of of IN cord-312431-de7zhswl 98 13 appropriate appropriate JJ cord-312431-de7zhswl 98 14 cell cell NN cord-312431-de7zhswl 98 15 lines line NNS cord-312431-de7zhswl 98 16 and and CC cord-312431-de7zhswl 98 17 incubated incubate VBN cord-312431-de7zhswl 98 18 at at IN cord-312431-de7zhswl 98 19 37 37 CD cord-312431-de7zhswl 98 20 ° ° NN cord-312431-de7zhswl 98 21 C c NN cord-312431-de7zhswl 98 22 for for IN cord-312431-de7zhswl 98 23 5 5 CD cord-312431-de7zhswl 98 24 - - SYM cord-312431-de7zhswl 98 25 7 7 CD cord-312431-de7zhswl 98 26 days day NNS cord-312431-de7zhswl 98 27 . . . cord-312431-de7zhswl 99 1 The the DT cord-312431-de7zhswl 99 2 development development NN cord-312431-de7zhswl 99 3 of of IN cord-312431-de7zhswl 99 4 cytopathic cytopathic JJ cord-312431-de7zhswl 99 5 effect effect NN cord-312431-de7zhswl 99 6 ( ( -LRB- cord-312431-de7zhswl 99 7 CPE CPE NNP cord-312431-de7zhswl 99 8 ) ) -RRB- cord-312431-de7zhswl 99 9 that that WDT cord-312431-de7zhswl 99 10 is be VBZ cord-312431-de7zhswl 99 11 indicative indicative JJ cord-312431-de7zhswl 99 12 of of IN cord-312431-de7zhswl 99 13 a a DT cord-312431-de7zhswl 99 14 viral viral JJ cord-312431-de7zhswl 99 15 infection infection NN cord-312431-de7zhswl 99 16 in in IN cord-312431-de7zhswl 99 17 the the DT cord-312431-de7zhswl 99 18 cell cell NN cord-312431-de7zhswl 99 19 cultures culture NNS cord-312431-de7zhswl 99 20 was be VBD cord-312431-de7zhswl 99 21 monitored monitor VBN cord-312431-de7zhswl 99 22 for for IN cord-312431-de7zhswl 99 23 up up IN cord-312431-de7zhswl 99 24 to to TO cord-312431-de7zhswl 99 25 7 7 CD cord-312431-de7zhswl 99 26 days day NNS cord-312431-de7zhswl 99 27 . . . cord-312431-de7zhswl 100 1 Presence presence NN cord-312431-de7zhswl 100 2 or or CC cord-312431-de7zhswl 100 3 absence absence NN cord-312431-de7zhswl 100 4 of of IN cord-312431-de7zhswl 100 5 CPE CPE NNP cord-312431-de7zhswl 100 6 ( ( -LRB- cord-312431-de7zhswl 100 7 loss loss NN cord-312431-de7zhswl 100 8 of of IN cord-312431-de7zhswl 100 9 cell cell NN cord-312431-de7zhswl 100 10 to to IN cord-312431-de7zhswl 100 11 cell cell NN cord-312431-de7zhswl 100 12 contact contact NN cord-312431-de7zhswl 100 13 and and CC cord-312431-de7zhswl 100 14 detachment detachment NN cord-312431-de7zhswl 100 15 ) ) -RRB- cord-312431-de7zhswl 100 16 was be VBD cord-312431-de7zhswl 100 17 confirmed confirm VBN cord-312431-de7zhswl 100 18 as as IN cord-312431-de7zhswl 100 19 described describe VBN cord-312431-de7zhswl 100 20 in in IN cord-312431-de7zhswl 100 21 US US NNP cord-312431-de7zhswl 100 22 EPA EPA NNP cord-312431-de7zhswl 100 23 [ [ -LRB- cord-312431-de7zhswl 100 24 43 43 CD cord-312431-de7zhswl 100 25 ] ] -RRB- cord-312431-de7zhswl 100 26 . . . cord-312431-de7zhswl 101 1 After after IN cord-312431-de7zhswl 101 2 three three CD cord-312431-de7zhswl 101 3 freeze freeze NN cord-312431-de7zhswl 101 4 - - HYPH cord-312431-de7zhswl 101 5 thaw thaw VB cord-312431-de7zhswl 101 6 cycles cycle NNS cord-312431-de7zhswl 101 7 , , , cord-312431-de7zhswl 101 8 CPE CPE NNP cord-312431-de7zhswl 101 9 positive positive JJ cord-312431-de7zhswl 101 10 and and CC cord-312431-de7zhswl 101 11 negative negative JJ cord-312431-de7zhswl 101 12 samples sample NNS cord-312431-de7zhswl 101 13 were be VBD cord-312431-de7zhswl 101 14 filtered filter VBN cord-312431-de7zhswl 101 15 through through IN cord-312431-de7zhswl 101 16 0.22 0.22 CD cord-312431-de7zhswl 101 17 mm mm NNP cord-312431-de7zhswl 101 18 syringe syringe NN cord-312431-de7zhswl 101 19 filters filter NNS cord-312431-de7zhswl 101 20 and and CC cord-312431-de7zhswl 101 21 were be VBD cord-312431-de7zhswl 101 22 inoculated inoculate VBN cord-312431-de7zhswl 101 23 in in IN cord-312431-de7zhswl 101 24 new new JJ cord-312431-de7zhswl 101 25 A549 A549 NNP cord-312431-de7zhswl 101 26 , , , cord-312431-de7zhswl 101 27 HEK293 HEK293 NNP cord-312431-de7zhswl 101 28 , , , cord-312431-de7zhswl 101 29 Hela Hela NNP cord-312431-de7zhswl 101 30 , , , cord-312431-de7zhswl 101 31 HepG2 HepG2 NNP cord-312431-de7zhswl 101 32 and and CC cord-312431-de7zhswl 101 33 PLC PLC NNP cord-312431-de7zhswl 101 34 / / SYM cord-312431-de7zhswl 101 35 PRF/5 PRF/5 NNP cord-312431-de7zhswl 101 36 cells cell NNS cord-312431-de7zhswl 101 37 for for IN cord-312431-de7zhswl 101 38 another another DT cord-312431-de7zhswl 101 39 seven seven CD cord-312431-de7zhswl 101 40 days day NNS cord-312431-de7zhswl 101 41 . . . cord-312431-de7zhswl 102 1 The the DT cord-312431-de7zhswl 102 2 CPE CPE NNP cord-312431-de7zhswl 102 3 of of IN cord-312431-de7zhswl 102 4 cell cell NN cord-312431-de7zhswl 102 5 culture culture NN cord-312431-de7zhswl 102 6 was be VBD cord-312431-de7zhswl 102 7 inspected inspect VBN cord-312431-de7zhswl 102 8 under under IN cord-312431-de7zhswl 102 9 an an DT cord-312431-de7zhswl 102 10 Olympus Olympus NNP cord-312431-de7zhswl 102 11 microscope microscope NN cord-312431-de7zhswl 102 12 at at IN cord-312431-de7zhswl 102 13 400Â 400â CD cord-312431-de7zhswl 102 14 magnification magnification NN cord-312431-de7zhswl 102 15 . . . cord-312431-de7zhswl 103 1 Samples sample NNS cord-312431-de7zhswl 103 2 that that WDT cord-312431-de7zhswl 103 3 showed show VBD cord-312431-de7zhswl 103 4 CPE CPE NNP cord-312431-de7zhswl 103 5 at at IN cord-312431-de7zhswl 103 6 the the DT cord-312431-de7zhswl 103 7 end end NN cord-312431-de7zhswl 103 8 of of IN cord-312431-de7zhswl 103 9 the the DT cord-312431-de7zhswl 103 10 confirmation confirmation NN cord-312431-de7zhswl 103 11 step step NN cord-312431-de7zhswl 103 12 were be VBD cord-312431-de7zhswl 103 13 reported report VBN cord-312431-de7zhswl 103 14 as as IN cord-312431-de7zhswl 103 15 positive positive JJ cord-312431-de7zhswl 103 16 for for IN cord-312431-de7zhswl 103 17 infectivity infectivity NN cord-312431-de7zhswl 103 18 . . . cord-312431-de7zhswl 104 1 The the DT cord-312431-de7zhswl 104 2 MTT MTT NNP cord-312431-de7zhswl 104 3 ( ( -LRB- cord-312431-de7zhswl 104 4 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl CD cord-312431-de7zhswl 104 5 tetrazolium tetrazolium NNP cord-312431-de7zhswl 104 6 bromide bromide NN cord-312431-de7zhswl 104 7 ) ) -RRB- cord-312431-de7zhswl 104 8 assay assay NN cord-312431-de7zhswl 104 9 was be VBD cord-312431-de7zhswl 104 10 performed perform VBN cord-312431-de7zhswl 104 11 using use VBG cord-312431-de7zhswl 104 12 the the DT cord-312431-de7zhswl 104 13 cell cell NN cord-312431-de7zhswl 104 14 lines line NNS cord-312431-de7zhswl 104 15 mentioned mention VBN cord-312431-de7zhswl 104 16 above above IN cord-312431-de7zhswl 104 17 [ [ -LRB- cord-312431-de7zhswl 104 18 44 44 CD cord-312431-de7zhswl 104 19 ] ] -RRB- cord-312431-de7zhswl 104 20 . . . cord-312431-de7zhswl 105 1 When when WRB cord-312431-de7zhswl 105 2 performing perform VBG cord-312431-de7zhswl 105 3 the the DT cord-312431-de7zhswl 105 4 MTT MTT NNP cord-312431-de7zhswl 105 5 assay assay NN cord-312431-de7zhswl 105 6 , , , cord-312431-de7zhswl 105 7 the the DT cord-312431-de7zhswl 105 8 cells cell NNS cord-312431-de7zhswl 105 9 were be VBD cord-312431-de7zhswl 105 10 plated plate VBN cord-312431-de7zhswl 105 11 into into IN cord-312431-de7zhswl 105 12 24-well 24-well CD cord-312431-de7zhswl 105 13 plates plate NNS cord-312431-de7zhswl 105 14 and and CC cord-312431-de7zhswl 105 15 infected infect VBN cord-312431-de7zhswl 105 16 by by IN cord-312431-de7zhswl 105 17 the the DT cord-312431-de7zhswl 105 18 viral viral JJ cord-312431-de7zhswl 105 19 samples sample NNS cord-312431-de7zhswl 105 20 ( ( -LRB- cord-312431-de7zhswl 105 21 each each DT cord-312431-de7zhswl 105 22 sample sample NN cord-312431-de7zhswl 105 23 in in IN cord-312431-de7zhswl 105 24 triplicate triplicate NN cord-312431-de7zhswl 105 25 ) ) -RRB- cord-312431-de7zhswl 105 26 approximately approximately RB cord-312431-de7zhswl 105 27 24 24 CD cord-312431-de7zhswl 105 28 h h NN cord-312431-de7zhswl 105 29 later later RB cord-312431-de7zhswl 105 30 . . . cord-312431-de7zhswl 106 1 After after IN cord-312431-de7zhswl 106 2 6 6 CD cord-312431-de7zhswl 106 3 - - SYM cord-312431-de7zhswl 106 4 7 7 CD cord-312431-de7zhswl 106 5 days day NNS cord-312431-de7zhswl 106 6 , , , cord-312431-de7zhswl 106 7 the the DT cord-312431-de7zhswl 106 8 medium medium NN cord-312431-de7zhswl 106 9 was be VBD cord-312431-de7zhswl 106 10 removed remove VBN cord-312431-de7zhswl 106 11 and and CC cord-312431-de7zhswl 106 12 MTT MTT NNP cord-312431-de7zhswl 106 13 solution solution NN cord-312431-de7zhswl 106 14 was be VBD cord-312431-de7zhswl 106 15 added add VBN cord-312431-de7zhswl 106 16 to to IN cord-312431-de7zhswl 106 17 the the DT cord-312431-de7zhswl 106 18 wells well NNS cord-312431-de7zhswl 106 19 , , , cord-312431-de7zhswl 106 20 and and CC cord-312431-de7zhswl 106 21 further further RB cord-312431-de7zhswl 106 22 incubated incubate VBN cord-312431-de7zhswl 106 23 at at IN cord-312431-de7zhswl 106 24 30 30 CD cord-312431-de7zhswl 106 25 ° ° NN cord-312431-de7zhswl 106 26 C c NN cord-312431-de7zhswl 106 27 for for IN cord-312431-de7zhswl 106 28 4 4 CD cord-312431-de7zhswl 106 29 h. h. NN cord-312431-de7zhswl 107 1 Thereafter Thereafter NNP cord-312431-de7zhswl 107 2 the the DT cord-312431-de7zhswl 107 3 MTT MTT NNP cord-312431-de7zhswl 107 4 solution solution NN cord-312431-de7zhswl 107 5 was be VBD cord-312431-de7zhswl 107 6 removed remove VBN cord-312431-de7zhswl 107 7 and and CC cord-312431-de7zhswl 107 8 DMSO DMSO NNP cord-312431-de7zhswl 107 9 was be VBD cord-312431-de7zhswl 107 10 added add VBN cord-312431-de7zhswl 107 11 to to IN cord-312431-de7zhswl 107 12 the the DT cord-312431-de7zhswl 107 13 wells well NNS cord-312431-de7zhswl 107 14 . . . cord-312431-de7zhswl 108 1 The the DT cord-312431-de7zhswl 108 2 cell cell NN cord-312431-de7zhswl 108 3 viability viability NN cord-312431-de7zhswl 108 4 ( ( -LRB- cord-312431-de7zhswl 108 5 % % NN cord-312431-de7zhswl 108 6 ) ) -RRB- cord-312431-de7zhswl 108 7 was be VBD cord-312431-de7zhswl 108 8 determined determine VBN cord-312431-de7zhswl 108 9 by by IN cord-312431-de7zhswl 108 10 the the DT cord-312431-de7zhswl 108 11 optical optical JJ cord-312431-de7zhswl 108 12 density density NN cord-312431-de7zhswl 108 13 of of IN cord-312431-de7zhswl 108 14 cells cell NNS cord-312431-de7zhswl 108 15 at at IN cord-312431-de7zhswl 108 16 570 570 CD cord-312431-de7zhswl 108 17 nm nm NN cord-312431-de7zhswl 108 18 using use VBG cord-312431-de7zhswl 108 19 a a DT cord-312431-de7zhswl 108 20 Mindray Mindray NNP cord-312431-de7zhswl 108 21 96A 96a CD cord-312431-de7zhswl 108 22 microplate microplate NN cord-312431-de7zhswl 108 23 reader reader NN cord-312431-de7zhswl 108 24 ( ( -LRB- cord-312431-de7zhswl 108 25 Vacutec Vacutec NNP cord-312431-de7zhswl 108 26 , , , cord-312431-de7zhswl 108 27 South South NNP cord-312431-de7zhswl 108 28 Africa Africa NNP cord-312431-de7zhswl 108 29 ) ) -RRB- cord-312431-de7zhswl 108 30 according accord VBG cord-312431-de7zhswl 108 31 to to IN cord-312431-de7zhswl 108 32 the the DT cord-312431-de7zhswl 108 33 method method NN cord-312431-de7zhswl 108 34 of of IN cord-312431-de7zhswl 108 35 Heldt Heldt NNP cord-312431-de7zhswl 108 36 et et NNP cord-312431-de7zhswl 108 37 al al NNP cord-312431-de7zhswl 108 38 . . . cord-312431-de7zhswl 109 1 [ [ -LRB- cord-312431-de7zhswl 109 2 44 44 CD cord-312431-de7zhswl 109 3 ] ] -RRB- cord-312431-de7zhswl 109 4 . . . cord-312431-de7zhswl 110 1 The the DT cord-312431-de7zhswl 110 2 CPE CPE NNP cord-312431-de7zhswl 110 3 was be VBD cord-312431-de7zhswl 110 4 calculated calculate VBN cord-312431-de7zhswl 110 5 as as IN cord-312431-de7zhswl 110 6 the the DT cord-312431-de7zhswl 110 7 percentage percentage NN cord-312431-de7zhswl 110 8 of of IN cord-312431-de7zhswl 110 9 cell cell NN cord-312431-de7zhswl 110 10 death death NN cord-312431-de7zhswl 110 11 due due IN cord-312431-de7zhswl 110 12 to to IN cord-312431-de7zhswl 110 13 the the DT cord-312431-de7zhswl 110 14 viral viral JJ cord-312431-de7zhswl 110 15 infection infection NN cord-312431-de7zhswl 110 16 comparing compare VBG cord-312431-de7zhswl 110 17 to to IN cord-312431-de7zhswl 110 18 the the DT cord-312431-de7zhswl 110 19 control control NN cord-312431-de7zhswl 110 20 . . . cord-312431-de7zhswl 111 1 Pearson Pearson NNP cord-312431-de7zhswl 111 2 's 's POS cord-312431-de7zhswl 111 3 correlation correlation NN cord-312431-de7zhswl 111 4 test test NN cord-312431-de7zhswl 111 5 was be VBD cord-312431-de7zhswl 111 6 used use VBN cord-312431-de7zhswl 111 7 to to TO cord-312431-de7zhswl 111 8 evaluate evaluate VB cord-312431-de7zhswl 111 9 the the DT cord-312431-de7zhswl 111 10 correlations correlation NNS cord-312431-de7zhswl 111 11 between between IN cord-312431-de7zhswl 111 12 the the DT cord-312431-de7zhswl 111 13 bacteriophages bacteriophage NNS cord-312431-de7zhswl 111 14 , , , cord-312431-de7zhswl 111 15 VLPs vlp NNS cord-312431-de7zhswl 111 16 and and CC cord-312431-de7zhswl 111 17 environmental environmental JJ cord-312431-de7zhswl 111 18 variables variable NNS cord-312431-de7zhswl 111 19 . . . cord-312431-de7zhswl 112 1 Data datum NNS cord-312431-de7zhswl 112 2 comparisons comparison NNS cord-312431-de7zhswl 112 3 were be VBD cord-312431-de7zhswl 112 4 performed perform VBN cord-312431-de7zhswl 112 5 using use VBG cord-312431-de7zhswl 112 6 analysis analysis NN cord-312431-de7zhswl 112 7 of of IN cord-312431-de7zhswl 112 8 variance variance NN cord-312431-de7zhswl 112 9 ( ( -LRB- cord-312431-de7zhswl 112 10 post post FW cord-312431-de7zhswl 112 11 hoc hoc FW cord-312431-de7zhswl 112 12 tests test NNS cord-312431-de7zhswl 112 13 ) ) -RRB- cord-312431-de7zhswl 112 14 and and CC cord-312431-de7zhswl 112 15 the the DT cord-312431-de7zhswl 112 16 Student Student NNP cord-312431-de7zhswl 112 17 's 's POS cord-312431-de7zhswl 112 18 t t NN cord-312431-de7zhswl 112 19 - - HYPH cord-312431-de7zhswl 112 20 test test NN cord-312431-de7zhswl 112 21 . . . cord-312431-de7zhswl 113 1 Probability probability NN cord-312431-de7zhswl 113 2 ( ( -LRB- cord-312431-de7zhswl 113 3 significant significant JJ cord-312431-de7zhswl 113 4 level level NN cord-312431-de7zhswl 113 5 ) ) -RRB- cord-312431-de7zhswl 113 6 was be VBD cord-312431-de7zhswl 113 7 set set VBN cord-312431-de7zhswl 113 8 at at IN cord-312431-de7zhswl 113 9 0.05 0.05 CD cord-312431-de7zhswl 113 10 . . . cord-312431-de7zhswl 114 1 The the DT cord-312431-de7zhswl 114 2 SPSS SPSS NNP cord-312431-de7zhswl 114 3 program program NN cord-312431-de7zhswl 114 4 version version NN cord-312431-de7zhswl 114 5 19 19 CD cord-312431-de7zhswl 114 6 ( ( -LRB- cord-312431-de7zhswl 114 7 SPSS SPSS NNP cord-312431-de7zhswl 114 8 , , , cord-312431-de7zhswl 114 9 Illinois Illinois NNP cord-312431-de7zhswl 114 10 ) ) -RRB- cord-312431-de7zhswl 114 11 was be VBD cord-312431-de7zhswl 114 12 used use VBN cord-312431-de7zhswl 114 13 for for IN cord-312431-de7zhswl 114 14 the the DT cord-312431-de7zhswl 114 15 statistical statistical JJ cord-312431-de7zhswl 114 16 analyses analysis NNS cord-312431-de7zhswl 114 17 [ [ -LRB- cord-312431-de7zhswl 114 18 45 45 CD cord-312431-de7zhswl 114 19 ] ] -RRB- cord-312431-de7zhswl 114 20 . . . cord-312431-de7zhswl 115 1 Canonical canonical JJ cord-312431-de7zhswl 115 2 correspondence correspondence NN cord-312431-de7zhswl 115 3 analysis analysis NN cord-312431-de7zhswl 115 4 ( ( -LRB- cord-312431-de7zhswl 115 5 CCA CCA NNP cord-312431-de7zhswl 115 6 ) ) -RRB- cord-312431-de7zhswl 115 7 was be VBD cord-312431-de7zhswl 115 8 used use VBN cord-312431-de7zhswl 115 9 to to TO cord-312431-de7zhswl 115 10 reveal reveal VB cord-312431-de7zhswl 115 11 the the DT cord-312431-de7zhswl 115 12 association association NN cord-312431-de7zhswl 115 13 amongst amongst IN cord-312431-de7zhswl 115 14 the the DT cord-312431-de7zhswl 115 15 bacteriophages bacteriophage NNS cord-312431-de7zhswl 115 16 , , , cord-312431-de7zhswl 115 17 VLPs vlp NNS cord-312431-de7zhswl 115 18 and and CC cord-312431-de7zhswl 115 19 the the DT cord-312431-de7zhswl 115 20 physical physical JJ cord-312431-de7zhswl 115 21 and and CC cord-312431-de7zhswl 115 22 chemical chemical JJ cord-312431-de7zhswl 115 23 water water NN cord-312431-de7zhswl 115 24 quality quality NN cord-312431-de7zhswl 115 25 variables variable NNS cord-312431-de7zhswl 115 26 , , , cord-312431-de7zhswl 115 27 which which WDT cord-312431-de7zhswl 115 28 were be VBD cord-312431-de7zhswl 115 29 measured measure VBN cord-312431-de7zhswl 115 30 from from IN cord-312431-de7zhswl 115 31 the the DT cord-312431-de7zhswl 115 32 same same JJ cord-312431-de7zhswl 115 33 sites site NNS cord-312431-de7zhswl 115 34 and and CC cord-312431-de7zhswl 115 35 seasons season NNS cord-312431-de7zhswl 115 36 in in IN cord-312431-de7zhswl 115 37 concurrent concurrent JJ cord-312431-de7zhswl 115 38 studies study NNS cord-312431-de7zhswl 115 39 performed perform VBN cord-312431-de7zhswl 115 40 in in IN cord-312431-de7zhswl 115 41 this this DT cord-312431-de7zhswl 115 42 laboratory laboratory NN cord-312431-de7zhswl 115 43 [ [ -LRB- cord-312431-de7zhswl 115 44 46 46 CD cord-312431-de7zhswl 115 45 ] ] -RRB- cord-312431-de7zhswl 115 46 , , , cord-312431-de7zhswl 115 47 with with IN cord-312431-de7zhswl 115 48 a a DT cord-312431-de7zhswl 115 49 view view NN cord-312431-de7zhswl 115 50 to to IN cord-312431-de7zhswl 115 51 defining define VBG cord-312431-de7zhswl 115 52 the the DT cord-312431-de7zhswl 115 53 significant significant JJ cord-312431-de7zhswl 115 54 variables variable NNS cord-312431-de7zhswl 115 55 accountable accountable JJ cord-312431-de7zhswl 115 56 for for IN cord-312431-de7zhswl 115 57 the the DT cord-312431-de7zhswl 115 58 observed observed JJ cord-312431-de7zhswl 115 59 spatial spatial JJ cord-312431-de7zhswl 115 60 and and CC cord-312431-de7zhswl 115 61 temporal temporal JJ cord-312431-de7zhswl 115 62 distribution distribution NN cord-312431-de7zhswl 115 63 of of IN cord-312431-de7zhswl 115 64 the the DT cord-312431-de7zhswl 115 65 communities community NNS cord-312431-de7zhswl 115 66 . . . cord-312431-de7zhswl 116 1 CCA CCA NNP cord-312431-de7zhswl 116 2 ordination ordination NN cord-312431-de7zhswl 116 3 allows allow VBZ cord-312431-de7zhswl 116 4 assessment assessment NN cord-312431-de7zhswl 116 5 of of IN cord-312431-de7zhswl 116 6 the the DT cord-312431-de7zhswl 116 7 relationships relationship NNS cord-312431-de7zhswl 116 8 between between IN cord-312431-de7zhswl 116 9 biological biological JJ cord-312431-de7zhswl 116 10 and and CC cord-312431-de7zhswl 116 11 environmental environmental JJ cord-312431-de7zhswl 116 12 data datum NNS cord-312431-de7zhswl 116 13 , , , cord-312431-de7zhswl 116 14 specifically specifically RB cord-312431-de7zhswl 116 15 the the DT cord-312431-de7zhswl 116 16 extent extent NN cord-312431-de7zhswl 116 17 to to TO cord-312431-de7zhswl 116 18 which which WDT cord-312431-de7zhswl 116 19 variation variation NN cord-312431-de7zhswl 116 20 in in IN cord-312431-de7zhswl 116 21 biological biological JJ cord-312431-de7zhswl 116 22 data datum NNS cord-312431-de7zhswl 116 23 can can MD cord-312431-de7zhswl 116 24 be be VB cord-312431-de7zhswl 116 25 accounted account VBN cord-312431-de7zhswl 116 26 for for IN cord-312431-de7zhswl 116 27 by by IN cord-312431-de7zhswl 116 28 the the DT cord-312431-de7zhswl 116 29 measured measure VBN cord-312431-de7zhswl 116 30 environmental environmental JJ cord-312431-de7zhswl 116 31 variables variable NNS cord-312431-de7zhswl 116 32 . . . cord-312431-de7zhswl 117 1 On on IN cord-312431-de7zhswl 117 2 CCA CCA NNP cord-312431-de7zhswl 117 3 ordinations ordination NNS cord-312431-de7zhswl 117 4 , , , cord-312431-de7zhswl 117 5 environmental environmental JJ cord-312431-de7zhswl 117 6 variables variable NNS cord-312431-de7zhswl 117 7 are be VBP cord-312431-de7zhswl 117 8 represented represent VBN cord-312431-de7zhswl 117 9 by by IN cord-312431-de7zhswl 117 10 arrows arrow NNS cord-312431-de7zhswl 117 11 whose whose WP$ cord-312431-de7zhswl 117 12 lengths length NNS cord-312431-de7zhswl 117 13 reflect reflect VBP cord-312431-de7zhswl 117 14 their -PRON- PRP$ cord-312431-de7zhswl 117 15 relative relative JJ cord-312431-de7zhswl 117 16 importance importance NN cord-312431-de7zhswl 117 17 in in IN cord-312431-de7zhswl 117 18 structuring structure VBG cord-312431-de7zhswl 117 19 the the DT cord-312431-de7zhswl 117 20 biological biological JJ cord-312431-de7zhswl 117 21 sample sample NN cord-312431-de7zhswl 117 22 data datum NNS cord-312431-de7zhswl 117 23 [ [ -LRB- cord-312431-de7zhswl 117 24 47 47 CD cord-312431-de7zhswl 117 25 ] ] -RRB- cord-312431-de7zhswl 117 26 . . . cord-312431-de7zhswl 118 1 A a DT cord-312431-de7zhswl 118 2 Monte Monte NNP cord-312431-de7zhswl 118 3 Carlo Carlo NNP cord-312431-de7zhswl 118 4 permutation permutation NN cord-312431-de7zhswl 118 5 test test NN cord-312431-de7zhswl 118 6 with with IN cord-312431-de7zhswl 118 7 499 499 CD cord-312431-de7zhswl 118 8 random random JJ cord-312431-de7zhswl 118 9 permutations permutation NNS cord-312431-de7zhswl 118 10 was be VBD cord-312431-de7zhswl 118 11 used use VBN cord-312431-de7zhswl 118 12 to to TO cord-312431-de7zhswl 118 13 establish establish VB cord-312431-de7zhswl 118 14 the the DT cord-312431-de7zhswl 118 15 environmental environmental JJ cord-312431-de7zhswl 118 16 axis axis NN cord-312431-de7zhswl 118 17 that that WDT cord-312431-de7zhswl 118 18 significantly significantly RB cord-312431-de7zhswl 118 19 correlated correlate VBD cord-312431-de7zhswl 118 20 with with IN cord-312431-de7zhswl 118 21 the the DT cord-312431-de7zhswl 118 22 biological biological JJ cord-312431-de7zhswl 118 23 variables variable NNS cord-312431-de7zhswl 118 24 . . . cord-312431-de7zhswl 119 1 CCA CCA NNP cord-312431-de7zhswl 119 2 analysis analysis NN cord-312431-de7zhswl 119 3 was be VBD cord-312431-de7zhswl 119 4 performed perform VBN cord-312431-de7zhswl 119 5 using use VBG cord-312431-de7zhswl 119 6 the the DT cord-312431-de7zhswl 119 7 computer computer NN cord-312431-de7zhswl 119 8 programme programme NN cord-312431-de7zhswl 119 9 Canoco Canoco NNP cord-312431-de7zhswl 119 10 for for IN cord-312431-de7zhswl 119 11 Windows Windows NNP cord-312431-de7zhswl 119 12 version version NN cord-312431-de7zhswl 119 13 4.5 4.5 CD cord-312431-de7zhswl 119 14 [ [ -LRB- cord-312431-de7zhswl 119 15 48 48 CD cord-312431-de7zhswl 119 16 ] ] -RRB- cord-312431-de7zhswl 119 17 . . . cord-312431-de7zhswl 120 1 Somatic somatic JJ cord-312431-de7zhswl 120 2 coliphage coliphage NN cord-312431-de7zhswl 120 3 counts count VBZ cord-312431-de7zhswl 120 4 ranged range VBD cord-312431-de7zhswl 120 5 from from IN cord-312431-de7zhswl 120 6 10 10 CD cord-312431-de7zhswl 120 7 pfu pfu NN cord-312431-de7zhswl 120 8 / / SYM cord-312431-de7zhswl 120 9 mL ml NN cord-312431-de7zhswl 120 10 ( ( -LRB- cord-312431-de7zhswl 120 11 U5-autumn U5-autumn NNP cord-312431-de7zhswl 120 12 ) ) -RRB- cord-312431-de7zhswl 120 13 to to IN cord-312431-de7zhswl 120 14 659 659 CD cord-312431-de7zhswl 120 15 pfu pfu NN cord-312431-de7zhswl 120 16 / / SYM cord-312431-de7zhswl 120 17 mL ml NN cord-312431-de7zhswl 120 18 ( ( -LRB- cord-312431-de7zhswl 120 19 U1-summer U1-summer NNP cord-312431-de7zhswl 120 20 ) ) -RRB- cord-312431-de7zhswl 120 21 and and CC cord-312431-de7zhswl 120 22 F F NNP cord-312431-de7zhswl 120 23 - - HYPH cord-312431-de7zhswl 120 24 RNA RNA NNP cord-312431-de7zhswl 120 25 coliphage coliphage NN cord-312431-de7zhswl 120 26 counts count VBZ cord-312431-de7zhswl 120 27 from from IN cord-312431-de7zhswl 120 28 0 0 CD cord-312431-de7zhswl 120 29 pfu pfu NN cord-312431-de7zhswl 120 30 / / SYM cord-312431-de7zhswl 120 31 mL ml NN cord-312431-de7zhswl 120 32 ( ( -LRB- cord-312431-de7zhswl 120 33 U5-autumn U5-autumn NNP cord-312431-de7zhswl 120 34 ) ) -RRB- cord-312431-de7zhswl 120 35 to to IN cord-312431-de7zhswl 120 36 550 550 CD cord-312431-de7zhswl 120 37 pfu pfu NN cord-312431-de7zhswl 120 38 / / SYM cord-312431-de7zhswl 120 39 mL ml NN cord-312431-de7zhswl 120 40 ( ( -LRB- cord-312431-de7zhswl 120 41 U2-summer U2-summer NNP cord-312431-de7zhswl 120 42 ) ) -RRB- cord-312431-de7zhswl 120 43 , , , cord-312431-de7zhswl 120 44 respectively respectively RB cord-312431-de7zhswl 120 45 ( ( -LRB- cord-312431-de7zhswl 120 46 Fig fig NN cord-312431-de7zhswl 120 47 . . . cord-312431-de7zhswl 120 48 3 3 CD cord-312431-de7zhswl 120 49 ) ) -RRB- cord-312431-de7zhswl 120 50 . . . cord-312431-de7zhswl 121 1 VLPs vlp NNS cord-312431-de7zhswl 121 2 were be VBD cord-312431-de7zhswl 121 3 detected detect VBN cord-312431-de7zhswl 121 4 using use VBG cord-312431-de7zhswl 121 5 EM EM NNP cord-312431-de7zhswl 121 6 ( ( -LRB- cord-312431-de7zhswl 121 7 electrostatic electrostatic JJ cord-312431-de7zhswl 121 8 force force NN cord-312431-de7zhswl 121 9 microscopy microscopy NNP cord-312431-de7zhswl 121 10 ) ) -RRB- cord-312431-de7zhswl 121 11 . . . cord-312431-de7zhswl 122 1 Point point NN cord-312431-de7zhswl 122 2 U1 u1 NN cord-312431-de7zhswl 122 3 during during IN cord-312431-de7zhswl 122 4 summer summer NN cord-312431-de7zhswl 122 5 had have VBD cord-312431-de7zhswl 122 6 the the DT cord-312431-de7zhswl 122 7 highest high JJS cord-312431-de7zhswl 122 8 population population NN cord-312431-de7zhswl 122 9 of of IN cord-312431-de7zhswl 122 10 2086 2086 CD cord-312431-de7zhswl 122 11 VLP VLP NNP cord-312431-de7zhswl 122 12 / / SYM cord-312431-de7zhswl 122 13 mL ml NN cord-312431-de7zhswl 122 14 and and CC cord-312431-de7zhswl 122 15 point point NN cord-312431-de7zhswl 122 16 U4 u4 NN cord-312431-de7zhswl 122 17 and and CC cord-312431-de7zhswl 122 18 U5 U5 NNP cord-312431-de7zhswl 122 19 had have VBD cord-312431-de7zhswl 122 20 the the DT cord-312431-de7zhswl 122 21 lowest low JJS cord-312431-de7zhswl 122 22 VLP VLP NNP cord-312431-de7zhswl 122 23 counts count NNS cord-312431-de7zhswl 122 24 of of IN cord-312431-de7zhswl 122 25 221.5 221.5 CD cord-312431-de7zhswl 122 26 VLP VLP NNP cord-312431-de7zhswl 122 27 / / SYM cord-312431-de7zhswl 122 28 mL mL NNP cord-312431-de7zhswl 122 29 ( ( -LRB- cord-312431-de7zhswl 122 30 U4winter u4winter NN cord-312431-de7zhswl 122 31 ) ) -RRB- cord-312431-de7zhswl 122 32 and and CC cord-312431-de7zhswl 122 33 190.1 190.1 CD cord-312431-de7zhswl 122 34 VLP VLP NNP cord-312431-de7zhswl 122 35 / / SYM cord-312431-de7zhswl 122 36 mL mL NNP cord-312431-de7zhswl 122 37 ( ( -LRB- cord-312431-de7zhswl 122 38 U5-autumn U5-autumn NNP cord-312431-de7zhswl 122 39 ) ) -RRB- cord-312431-de7zhswl 122 40 , , , cord-312431-de7zhswl 122 41 respectively respectively RB cord-312431-de7zhswl 122 42 . . . cord-312431-de7zhswl 123 1 Mean mean JJ cord-312431-de7zhswl 123 2 concentrations concentration NNS cord-312431-de7zhswl 123 3 of of IN cord-312431-de7zhswl 123 4 somatic somatic JJ cord-312431-de7zhswl 123 5 coliphages coliphage NNS cord-312431-de7zhswl 123 6 and and CC cord-312431-de7zhswl 123 7 F F NNP cord-312431-de7zhswl 123 8 - - HYPH cord-312431-de7zhswl 123 9 RNA RNA NNP cord-312431-de7zhswl 123 10 coliphages coliphage NNS cord-312431-de7zhswl 123 11 were be VBD cord-312431-de7zhswl 123 12 comparable comparable JJ cord-312431-de7zhswl 123 13 between between IN cord-312431-de7zhswl 123 14 the the DT cord-312431-de7zhswl 123 15 autumn autumn NN cord-312431-de7zhswl 123 16 and and CC cord-312431-de7zhswl 123 17 spring spring NN cord-312431-de7zhswl 123 18 seasons season NNS cord-312431-de7zhswl 123 19 and and CC cord-312431-de7zhswl 123 20 correlation correlation NN cord-312431-de7zhswl 123 21 analysis analysis NN cord-312431-de7zhswl 123 22 resulted result VBD cord-312431-de7zhswl 123 23 in in IN cord-312431-de7zhswl 123 24 a a DT cord-312431-de7zhswl 123 25 strong strong JJ cord-312431-de7zhswl 123 26 Pearson Pearson NNP cord-312431-de7zhswl 123 27 's 's POS cord-312431-de7zhswl 123 28 correlation correlation NN cord-312431-de7zhswl 123 29 coefficient coefficient NN cord-312431-de7zhswl 123 30 of of IN cord-312431-de7zhswl 123 31 0.977 0.977 CD cord-312431-de7zhswl 124 1 ( ( -LRB- cord-312431-de7zhswl 124 2 p p NN cord-312431-de7zhswl 124 3 < < XX cord-312431-de7zhswl 124 4 0.01 0.01 CD cord-312431-de7zhswl 124 5 ) ) -RRB- cord-312431-de7zhswl 124 6 . . . cord-312431-de7zhswl 125 1 These these DT cord-312431-de7zhswl 125 2 coliphages coliphage NNS cord-312431-de7zhswl 125 3 had have VBD cord-312431-de7zhswl 125 4 inverse inverse JJ cord-312431-de7zhswl 125 5 correlations correlation NNS cord-312431-de7zhswl 125 6 ( ( -LRB- cord-312431-de7zhswl 125 7 r r NNP cord-312431-de7zhswl 125 8 ¼ ¼ NNP cord-312431-de7zhswl 125 9 À0.536 à0.536 NN cord-312431-de7zhswl 125 10 ) ) -RRB- cord-312431-de7zhswl 125 11 with with IN cord-312431-de7zhswl 125 12 the the DT cord-312431-de7zhswl 125 13 VLPs vlp NNS cord-312431-de7zhswl 125 14 detected detect VBN cord-312431-de7zhswl 125 15 during during IN cord-312431-de7zhswl 125 16 autumn autumn NN cord-312431-de7zhswl 125 17 but but CC cord-312431-de7zhswl 125 18 correlated correlate VBN cord-312431-de7zhswl 125 19 ( ( -LRB- cord-312431-de7zhswl 125 20 r r NNP cord-312431-de7zhswl 125 21 ¼ ¼ CD cord-312431-de7zhswl 125 22 0.795 0.795 CD cord-312431-de7zhswl 125 23 ) ) -RRB- cord-312431-de7zhswl 125 24 with with IN cord-312431-de7zhswl 125 25 the the DT cord-312431-de7zhswl 125 26 VLPs vlp NNS cord-312431-de7zhswl 125 27 found find VBN cord-312431-de7zhswl 125 28 during during IN cord-312431-de7zhswl 125 29 spring spring NN cord-312431-de7zhswl 125 30 along along IN cord-312431-de7zhswl 125 31 the the DT cord-312431-de7zhswl 125 32 Umgeni Umgeni NNP cord-312431-de7zhswl 125 33 River River NNP cord-312431-de7zhswl 125 34 . . . cord-312431-de7zhswl 126 1 In in IN cord-312431-de7zhswl 126 2 this this DT cord-312431-de7zhswl 126 3 study study NN cord-312431-de7zhswl 126 4 , , , cord-312431-de7zhswl 126 5 CCA CCA NNP cord-312431-de7zhswl 126 6 was be VBD cord-312431-de7zhswl 126 7 used use VBN cord-312431-de7zhswl 126 8 to to TO cord-312431-de7zhswl 126 9 investigate investigate VB cord-312431-de7zhswl 126 10 the the DT cord-312431-de7zhswl 126 11 relationships relationship NNS cord-312431-de7zhswl 126 12 between between IN cord-312431-de7zhswl 126 13 the the DT cord-312431-de7zhswl 126 14 water water NN cord-312431-de7zhswl 126 15 quality quality NN cord-312431-de7zhswl 126 16 variables variable NNS cord-312431-de7zhswl 126 17 and and CC cord-312431-de7zhswl 126 18 the the DT cord-312431-de7zhswl 126 19 VLPs vlp NNS cord-312431-de7zhswl 126 20 , , , cord-312431-de7zhswl 126 21 as as RB cord-312431-de7zhswl 126 22 well well RB cord-312431-de7zhswl 126 23 as as IN cord-312431-de7zhswl 126 24 the the DT cord-312431-de7zhswl 126 25 relationships relationship NNS cord-312431-de7zhswl 126 26 between between IN cord-312431-de7zhswl 126 27 the the DT cord-312431-de7zhswl 126 28 bacteriophages bacteriophage NNS cord-312431-de7zhswl 126 29 and and CC cord-312431-de7zhswl 126 30 the the DT cord-312431-de7zhswl 126 31 physico physico NN cord-312431-de7zhswl 126 32 - - HYPH cord-312431-de7zhswl 126 33 chemical chemical NN cord-312431-de7zhswl 126 34 parameters parameter NNS cord-312431-de7zhswl 126 35 at at IN cord-312431-de7zhswl 126 36 all all DT cord-312431-de7zhswl 126 37 sites site NNS cord-312431-de7zhswl 126 38 and and CC cord-312431-de7zhswl 126 39 seasons season NNS cord-312431-de7zhswl 126 40 . . . cord-312431-de7zhswl 127 1 CCA CCA NNP cord-312431-de7zhswl 127 2 ordination ordination NN cord-312431-de7zhswl 127 3 plot plot NN cord-312431-de7zhswl 127 4 ( ( -LRB- cord-312431-de7zhswl 127 5 Fig Fig NNP cord-312431-de7zhswl 127 6 . . . cord-312431-de7zhswl 127 7 4 4 LS cord-312431-de7zhswl 127 8 ) ) -RRB- cord-312431-de7zhswl 127 9 revealed reveal VBD cord-312431-de7zhswl 127 10 strong strong JJ cord-312431-de7zhswl 127 11 relationships relationship NNS cord-312431-de7zhswl 127 12 between between IN cord-312431-de7zhswl 127 13 the the DT cord-312431-de7zhswl 127 14 overall overall JJ cord-312431-de7zhswl 127 15 VLP VLP NNP cord-312431-de7zhswl 127 16 and and CC cord-312431-de7zhswl 127 17 phage phage NN cord-312431-de7zhswl 127 18 populations population NNS cord-312431-de7zhswl 127 19 at at IN cord-312431-de7zhswl 127 20 different different JJ cord-312431-de7zhswl 127 21 sites site NNS cord-312431-de7zhswl 127 22 and and CC cord-312431-de7zhswl 127 23 seasons season NNS cord-312431-de7zhswl 127 24 measured measure VBN cord-312431-de7zhswl 127 25 as as RB cord-312431-de7zhswl 127 26 well well RB cord-312431-de7zhswl 127 27 as as IN cord-312431-de7zhswl 127 28 with with IN cord-312431-de7zhswl 127 29 the the DT cord-312431-de7zhswl 127 30 physicochemical physicochemical JJ cord-312431-de7zhswl 127 31 water water NN cord-312431-de7zhswl 127 32 quality quality NN cord-312431-de7zhswl 127 33 variables variable NNS cord-312431-de7zhswl 127 34 . . . cord-312431-de7zhswl 128 1 CCA CCA NNP cord-312431-de7zhswl 128 2 plot plot NN cord-312431-de7zhswl 128 3 ( ( -LRB- cord-312431-de7zhswl 128 4 blue blue JJ cord-312431-de7zhswl 128 5 circle circle NN cord-312431-de7zhswl 128 6 ) ) -RRB- cord-312431-de7zhswl 128 7 , , , cord-312431-de7zhswl 128 8 temperature temperature NN cord-312431-de7zhswl 128 9 , , , cord-312431-de7zhswl 128 10 biochemical biochemical JJ cord-312431-de7zhswl 128 11 oxygen oxygen NN cord-312431-de7zhswl 128 12 demand demand NN cord-312431-de7zhswl 128 13 in in IN cord-312431-de7zhswl 128 14 five five CD cord-312431-de7zhswl 128 15 days day NNS cord-312431-de7zhswl 128 16 , , , cord-312431-de7zhswl 128 17 turbidity turbidity NN cord-312431-de7zhswl 128 18 , , , cord-312431-de7zhswl 128 19 pH ph NN cord-312431-de7zhswl 128 20 , , , cord-312431-de7zhswl 128 21 conductivity conductivity NN cord-312431-de7zhswl 128 22 , , , cord-312431-de7zhswl 128 23 orthophosphate orthophosphate JJ cord-312431-de7zhswl 128 24 and and CC cord-312431-de7zhswl 128 25 sulphate sulphate NN cord-312431-de7zhswl 128 26 were be VBD cord-312431-de7zhswl 128 27 the the DT cord-312431-de7zhswl 128 28 most most RBS cord-312431-de7zhswl 128 29 important important JJ cord-312431-de7zhswl 128 30 variables variable NNS cord-312431-de7zhswl 128 31 ( ( -LRB- cord-312431-de7zhswl 128 32 Fig Fig NNP cord-312431-de7zhswl 128 33 . . NNP cord-312431-de7zhswl 128 34 4 4 CD cord-312431-de7zhswl 128 35 , , , cord-312431-de7zhswl 128 36 long long JJ cord-312431-de7zhswl 128 37 arrows arrow NNS cord-312431-de7zhswl 128 38 ) ) -RRB- cord-312431-de7zhswl 128 39 that that WDT cord-312431-de7zhswl 128 40 impacted impact VBD cord-312431-de7zhswl 128 41 the the DT cord-312431-de7zhswl 128 42 community community NN cord-312431-de7zhswl 128 43 structures structure NNS cord-312431-de7zhswl 128 44 significantly significantly RB cord-312431-de7zhswl 128 45 at at IN cord-312431-de7zhswl 128 46 sites site NNS cord-312431-de7zhswl 128 47 1 1 CD cord-312431-de7zhswl 128 48 , , , cord-312431-de7zhswl 128 49 2 2 CD cord-312431-de7zhswl 128 50 and and CC cord-312431-de7zhswl 128 51 3 3 CD cord-312431-de7zhswl 128 52 during during IN cord-312431-de7zhswl 128 53 the the DT cord-312431-de7zhswl 128 54 autumn autumn NN cord-312431-de7zhswl 128 55 , , , cord-312431-de7zhswl 128 56 summer summer NN cord-312431-de7zhswl 128 57 and and CC cord-312431-de7zhswl 128 58 spring spring NN cord-312431-de7zhswl 128 59 seasons season NNS cord-312431-de7zhswl 128 60 . . . cord-312431-de7zhswl 129 1 Site site NN cord-312431-de7zhswl 129 2 2 2 CD cord-312431-de7zhswl 129 3 , , , cord-312431-de7zhswl 129 4 4 4 CD cord-312431-de7zhswl 129 5 , , , cord-312431-de7zhswl 129 6 5 5 CD cord-312431-de7zhswl 129 7 during during IN cord-312431-de7zhswl 129 8 autumn autumn NN cord-312431-de7zhswl 129 9 , , , cord-312431-de7zhswl 129 10 winter winter NN cord-312431-de7zhswl 129 11 and and CC cord-312431-de7zhswl 129 12 spring spring NN cord-312431-de7zhswl 129 13 seasons season NNS cord-312431-de7zhswl 129 14 correlated correlate VBN cord-312431-de7zhswl 129 15 with with IN cord-312431-de7zhswl 129 16 the the DT cord-312431-de7zhswl 129 17 nitrate nitrate NN cord-312431-de7zhswl 129 18 / / SYM cord-312431-de7zhswl 129 19 nitrite nitrite NN cord-312431-de7zhswl 129 20 profiles profile NNS cord-312431-de7zhswl 129 21 ( ( -LRB- cord-312431-de7zhswl 129 22 green green JJ cord-312431-de7zhswl 129 23 circle circle NN cord-312431-de7zhswl 129 24 ) ) -RRB- cord-312431-de7zhswl 129 25 . . . cord-312431-de7zhswl 130 1 Site site NN cord-312431-de7zhswl 130 2 1 1 CD cord-312431-de7zhswl 130 3 and and CC cord-312431-de7zhswl 130 4 3 3 CD cord-312431-de7zhswl 130 5 in in IN cord-312431-de7zhswl 130 6 autumn autumn NN cord-312431-de7zhswl 130 7 and and CC cord-312431-de7zhswl 130 8 winter winter NN cord-312431-de7zhswl 130 9 showed show VBD cord-312431-de7zhswl 130 10 no no DT cord-312431-de7zhswl 130 11 correlations correlation NNS cord-312431-de7zhswl 130 12 with with IN cord-312431-de7zhswl 130 13 the the DT cord-312431-de7zhswl 130 14 rest rest NN cord-312431-de7zhswl 130 15 of of IN cord-312431-de7zhswl 130 16 the the DT cord-312431-de7zhswl 130 17 sites site NNS cord-312431-de7zhswl 130 18 and and CC cord-312431-de7zhswl 130 19 variables variable NNS cord-312431-de7zhswl 130 20 measured measure VBN cord-312431-de7zhswl 130 21 . . . cord-312431-de7zhswl 131 1 CCA CCA NNP cord-312431-de7zhswl 131 2 axis axis NN cord-312431-de7zhswl 131 3 1 1 CD cord-312431-de7zhswl 132 1 ( ( -LRB- cord-312431-de7zhswl 132 2 Fig Fig NNP cord-312431-de7zhswl 132 3 . . NNP cord-312431-de7zhswl 132 4 4 4 LS cord-312431-de7zhswl 132 5 ) ) -RRB- cord-312431-de7zhswl 132 6 accounted account VBD cord-312431-de7zhswl 132 7 for for IN cord-312431-de7zhswl 132 8 77.6 77.6 CD cord-312431-de7zhswl 132 9 % % NN cord-312431-de7zhswl 132 10 of of IN cord-312431-de7zhswl 132 11 total total JJ cord-312431-de7zhswl 132 12 variance variance NN cord-312431-de7zhswl 132 13 of of IN cord-312431-de7zhswl 132 14 the the DT cord-312431-de7zhswl 132 15 species species NN cord-312431-de7zhswl 132 16 data datum NNS cord-312431-de7zhswl 132 17 set set VBN cord-312431-de7zhswl 132 18 and and CC cord-312431-de7zhswl 132 19 in in IN cord-312431-de7zhswl 132 20 total total JJ cord-312431-de7zhswl 132 21 the the DT cord-312431-de7zhswl 132 22 speciesenvironment speciesenvironment NN cord-312431-de7zhswl 132 23 relation relation NN cord-312431-de7zhswl 132 24 accounted account VBD cord-312431-de7zhswl 132 25 for for IN cord-312431-de7zhswl 132 26 94.1 94.1 CD cord-312431-de7zhswl 132 27 % % NN cord-312431-de7zhswl 132 28 of of IN cord-312431-de7zhswl 132 29 the the DT cord-312431-de7zhswl 132 30 cumulative cumulative JJ cord-312431-de7zhswl 132 31 variance variance NN cord-312431-de7zhswl 132 32 suggesting suggest VBG cord-312431-de7zhswl 132 33 that that IN cord-312431-de7zhswl 132 34 there there EX cord-312431-de7zhswl 132 35 may may MD cord-312431-de7zhswl 132 36 be be VB cord-312431-de7zhswl 132 37 a a DT cord-312431-de7zhswl 132 38 correlation correlation NN cord-312431-de7zhswl 132 39 between between IN cord-312431-de7zhswl 132 40 the the DT cord-312431-de7zhswl 132 41 VLP VLP NNP cord-312431-de7zhswl 132 42 and and CC cord-312431-de7zhswl 132 43 phage phage NN cord-312431-de7zhswl 132 44 populations population NNS cord-312431-de7zhswl 132 45 at at IN cord-312431-de7zhswl 132 46 the the DT cord-312431-de7zhswl 132 47 various various JJ cord-312431-de7zhswl 132 48 sites site NNS cord-312431-de7zhswl 132 49 and and CC cord-312431-de7zhswl 132 50 water water NN cord-312431-de7zhswl 132 51 quality quality NN cord-312431-de7zhswl 132 52 variables variable NNS cord-312431-de7zhswl 132 53 during during IN cord-312431-de7zhswl 132 54 all all DT cord-312431-de7zhswl 132 55 seasons season NNS cord-312431-de7zhswl 132 56 . . . cord-312431-de7zhswl 133 1 Positive positive JJ cord-312431-de7zhswl 133 2 significant significant JJ cord-312431-de7zhswl 133 3 ( ( -LRB- cord-312431-de7zhswl 133 4 p p NN cord-312431-de7zhswl 133 5 < < XX cord-312431-de7zhswl 133 6 0.05 0.05 CD cord-312431-de7zhswl 133 7 ) ) -RRB- cord-312431-de7zhswl 134 1 Pearson Pearson NNP cord-312431-de7zhswl 134 2 correlation correlation NN cord-312431-de7zhswl 134 3 of of IN cord-312431-de7zhswl 134 4 species specie NNS cord-312431-de7zhswl 134 5 and and CC cord-312431-de7zhswl 134 6 environmental environmental JJ cord-312431-de7zhswl 134 7 scores score NNS cord-312431-de7zhswl 134 8 for for IN cord-312431-de7zhswl 134 9 axis axis NN cord-312431-de7zhswl 134 10 one one CD cord-312431-de7zhswl 134 11 and and CC cord-312431-de7zhswl 134 12 two two CD cord-312431-de7zhswl 134 13 were be VBD cord-312431-de7zhswl 134 14 r r NNP cord-312431-de7zhswl 134 15 ¼ ¼ CD cord-312431-de7zhswl 134 16 0.955 0.955 CD cord-312431-de7zhswl 134 17 and and CC cord-312431-de7zhswl 134 18 0.962 0.962 CD cord-312431-de7zhswl 134 19 , , , cord-312431-de7zhswl 134 20 respectively respectively RB cord-312431-de7zhswl 134 21 . . . cord-312431-de7zhswl 135 1 The the DT cord-312431-de7zhswl 135 2 ordination ordination NN cord-312431-de7zhswl 135 3 plot plot NN cord-312431-de7zhswl 135 4 revealed reveal VBD cord-312431-de7zhswl 135 5 that that IN cord-312431-de7zhswl 135 6 the the DT cord-312431-de7zhswl 135 7 Somatic somatic JJ cord-312431-de7zhswl 135 8 phage phage NN cord-312431-de7zhswl 135 9 , , , cord-312431-de7zhswl 135 10 F F NNP cord-312431-de7zhswl 135 11 - - HYPH cord-312431-de7zhswl 135 12 RNA RNA NNP cord-312431-de7zhswl 135 13 phage phage NN cord-312431-de7zhswl 135 14 and and CC cord-312431-de7zhswl 135 15 VLPs vlp NNS cord-312431-de7zhswl 135 16 had have VBD cord-312431-de7zhswl 135 17 little little JJ cord-312431-de7zhswl 135 18 relationships relationship NNS cord-312431-de7zhswl 135 19 with with IN cord-312431-de7zhswl 135 20 each each DT cord-312431-de7zhswl 135 21 other other JJ cord-312431-de7zhswl 135 22 and and CC cord-312431-de7zhswl 135 23 existed exist VBD cord-312431-de7zhswl 135 24 independently independently RB cord-312431-de7zhswl 135 25 ( ( -LRB- cord-312431-de7zhswl 135 26 Fig Fig NNP cord-312431-de7zhswl 135 27 . . . cord-312431-de7zhswl 135 28 5 5 CD cord-312431-de7zhswl 135 29 ) ) -RRB- cord-312431-de7zhswl 135 30 . . . cord-312431-de7zhswl 136 1 The the DT cord-312431-de7zhswl 136 2 sample sample NN cord-312431-de7zhswl 136 3 scores score NNS cord-312431-de7zhswl 136 4 were be VBD cord-312431-de7zhswl 136 5 scattered scatter VBN cord-312431-de7zhswl 136 6 in in IN cord-312431-de7zhswl 136 7 the the DT cord-312431-de7zhswl 136 8 ordination ordination NN cord-312431-de7zhswl 136 9 with with IN cord-312431-de7zhswl 136 10 a a DT cord-312431-de7zhswl 136 11 number number NN cord-312431-de7zhswl 136 12 of of IN cord-312431-de7zhswl 136 13 denser dense JJR cord-312431-de7zhswl 136 14 clusters cluster NNS cord-312431-de7zhswl 136 15 ( ( -LRB- cord-312431-de7zhswl 136 16 physico physico NN cord-312431-de7zhswl 136 17 - - HYPH cord-312431-de7zhswl 136 18 chemical chemical NN cord-312431-de7zhswl 136 19 ) ) -RRB- cord-312431-de7zhswl 136 20 spread spread VBD cord-312431-de7zhswl 136 21 out out RP cord-312431-de7zhswl 136 22 around around IN cord-312431-de7zhswl 136 23 the the DT cord-312431-de7zhswl 136 24 origin origin NN cord-312431-de7zhswl 136 25 . . . cord-312431-de7zhswl 137 1 CCA CCA NNP cord-312431-de7zhswl 137 2 axis axis NN cord-312431-de7zhswl 137 3 1 1 CD cord-312431-de7zhswl 138 1 ( ( -LRB- cord-312431-de7zhswl 138 2 Fig Fig NNP cord-312431-de7zhswl 138 3 . . NNP cord-312431-de7zhswl 138 4 5 5 LS cord-312431-de7zhswl 138 5 ) ) -RRB- cord-312431-de7zhswl 138 6 accounted account VBD cord-312431-de7zhswl 138 7 for for IN cord-312431-de7zhswl 138 8 60.5 60.5 CD cord-312431-de7zhswl 138 9 % % NN cord-312431-de7zhswl 138 10 of of IN cord-312431-de7zhswl 138 11 total total JJ cord-312431-de7zhswl 138 12 variance variance NN cord-312431-de7zhswl 138 13 of of IN cord-312431-de7zhswl 138 14 the the DT cord-312431-de7zhswl 138 15 species species NN cord-312431-de7zhswl 138 16 data datum NNS cord-312431-de7zhswl 138 17 set set VBN cord-312431-de7zhswl 138 18 and and CC cord-312431-de7zhswl 138 19 in in IN cord-312431-de7zhswl 138 20 total total NN cord-312431-de7zhswl 138 21 , , , cord-312431-de7zhswl 138 22 the the DT cord-312431-de7zhswl 138 23 speciesenvironment speciesenvironment NN cord-312431-de7zhswl 138 24 relation relation NN cord-312431-de7zhswl 138 25 ordination ordination NN cord-312431-de7zhswl 138 26 accounted account VBD cord-312431-de7zhswl 138 27 for for IN cord-312431-de7zhswl 138 28 83.7 83.7 CD cord-312431-de7zhswl 138 29 % % NN cord-312431-de7zhswl 138 30 of of IN cord-312431-de7zhswl 138 31 the the DT cord-312431-de7zhswl 138 32 cumulative cumulative JJ cord-312431-de7zhswl 138 33 variance variance NN cord-312431-de7zhswl 138 34 . . . cord-312431-de7zhswl 139 1 Eigen Eigen NNP cord-312431-de7zhswl 139 2 values value NNS cord-312431-de7zhswl 139 3 for for IN cord-312431-de7zhswl 139 4 axis axis NN cord-312431-de7zhswl 139 5 point point NN cord-312431-de7zhswl 139 6 one one CD cord-312431-de7zhswl 139 7 and and CC cord-312431-de7zhswl 139 8 two two CD cord-312431-de7zhswl 139 9 were be VBD cord-312431-de7zhswl 139 10 0.043 0.043 NNS cord-312431-de7zhswl 139 11 and and CC cord-312431-de7zhswl 139 12 0.021 0.021 CD cord-312431-de7zhswl 139 13 , , , cord-312431-de7zhswl 139 14 respectively respectively RB cord-312431-de7zhswl 139 15 . . . cord-312431-de7zhswl 140 1 Positive positive JJ cord-312431-de7zhswl 140 2 significant significant JJ cord-312431-de7zhswl 140 3 ( ( -LRB- cord-312431-de7zhswl 140 4 p p NN cord-312431-de7zhswl 140 5 < < XX cord-312431-de7zhswl 140 6 0.05 0.05 CD cord-312431-de7zhswl 140 7 ) ) -RRB- cord-312431-de7zhswl 141 1 Pearson Pearson NNP cord-312431-de7zhswl 141 2 correlation correlation NN cord-312431-de7zhswl 141 3 of of IN cord-312431-de7zhswl 141 4 species specie NNS cord-312431-de7zhswl 141 5 and and CC cord-312431-de7zhswl 141 6 environmental environmental JJ cord-312431-de7zhswl 141 7 scores score NNS cord-312431-de7zhswl 141 8 for for IN cord-312431-de7zhswl 141 9 axis axis NN cord-312431-de7zhswl 141 10 one one CD cord-312431-de7zhswl 141 11 and and CC cord-312431-de7zhswl 141 12 two two CD cord-312431-de7zhswl 141 13 were be VBD cord-312431-de7zhswl 141 14 r r NNP cord-312431-de7zhswl 141 15 ¼ ¼ CD cord-312431-de7zhswl 141 16 0.759 0.759 CD cord-312431-de7zhswl 141 17 and and CC cord-312431-de7zhswl 141 18 0.980 0.980 CD cord-312431-de7zhswl 141 19 , , , cord-312431-de7zhswl 141 20 respectively respectively RB cord-312431-de7zhswl 141 21 . . . cord-312431-de7zhswl 142 1 Table table NN cord-312431-de7zhswl 142 2 1 1 CD cord-312431-de7zhswl 142 3 classifies classify VBZ cord-312431-de7zhswl 142 4 the the DT cord-312431-de7zhswl 142 5 different different JJ cord-312431-de7zhswl 142 6 type type NN cord-312431-de7zhswl 142 7 of of IN cord-312431-de7zhswl 142 8 bacteriophage bacteriophage NN cord-312431-de7zhswl 142 9 that that WDT cord-312431-de7zhswl 142 10 could could MD cord-312431-de7zhswl 142 11 be be VB cord-312431-de7zhswl 142 12 detected detect VBN cord-312431-de7zhswl 142 13 in in IN cord-312431-de7zhswl 142 14 the the DT cord-312431-de7zhswl 142 15 Umgeni Umgeni NNP cord-312431-de7zhswl 142 16 River River NNP cord-312431-de7zhswl 142 17 by by IN cord-312431-de7zhswl 142 18 TEM TEM NNP cord-312431-de7zhswl 142 19 . . . cord-312431-de7zhswl 143 1 Most Most JJS cord-312431-de7zhswl 143 2 of of IN cord-312431-de7zhswl 143 3 the the DT cord-312431-de7zhswl 143 4 detected detect VBN cord-312431-de7zhswl 143 5 phages phage NNS cord-312431-de7zhswl 143 6 ( ( -LRB- cord-312431-de7zhswl 143 7 33 33 CD cord-312431-de7zhswl 143 8 % % NN cord-312431-de7zhswl 143 9 ) ) -RRB- cord-312431-de7zhswl 143 10 had have VBD cord-312431-de7zhswl 143 11 isometric isometric JJ cord-312431-de7zhswl 143 12 heads head NNS cord-312431-de7zhswl 143 13 and and CC cord-312431-de7zhswl 143 14 long long JJ cord-312431-de7zhswl 143 15 non non JJ cord-312431-de7zhswl 143 16 - - JJ cord-312431-de7zhswl 143 17 contractile contractile JJ cord-312431-de7zhswl 143 18 tails tail NNS cord-312431-de7zhswl 143 19 , , , cord-312431-de7zhswl 143 20 belonging belong VBG cord-312431-de7zhswl 143 21 to to IN cord-312431-de7zhswl 143 22 morphotype morphotype NN cord-312431-de7zhswl 143 23 B1 b1 NN cord-312431-de7zhswl 143 24 ( ( -LRB- cord-312431-de7zhswl 143 25 Siphoviridae Siphoviridae NNP cord-312431-de7zhswl 143 26 ) ) -RRB- cord-312431-de7zhswl 144 1 ( ( -LRB- cord-312431-de7zhswl 144 2 Tab Tab NNP cord-312431-de7zhswl 144 3 . . . cord-312431-de7zhswl 144 4 1 1 CD cord-312431-de7zhswl 144 5 ) ) -RRB- cord-312431-de7zhswl 144 6 . . . cord-312431-de7zhswl 145 1 Members member NNS cord-312431-de7zhswl 145 2 of of IN cord-312431-de7zhswl 145 3 morphotypes morphotype NNS cord-312431-de7zhswl 145 4 A1 A1 NNP cord-312431-de7zhswl 145 5 ( ( -LRB- cord-312431-de7zhswl 145 6 Myoviridae Myoviridae NNP cord-312431-de7zhswl 145 7 ) ) -RRB- cord-312431-de7zhswl 145 8 were be VBD cord-312431-de7zhswl 145 9 detected detect VBN cord-312431-de7zhswl 145 10 in in IN cord-312431-de7zhswl 145 11 25 25 CD cord-312431-de7zhswl 145 12 % % NN cord-312431-de7zhswl 145 13 of of IN cord-312431-de7zhswl 145 14 the the DT cord-312431-de7zhswl 145 15 samples sample NNS cord-312431-de7zhswl 145 16 , , , cord-312431-de7zhswl 145 17 and and CC cord-312431-de7zhswl 145 18 C1 C1 NNP cord-312431-de7zhswl 145 19 ( ( -LRB- cord-312431-de7zhswl 145 20 Podoviridae Podoviridae NNP cord-312431-de7zhswl 145 21 ) ) -RRB- cord-312431-de7zhswl 145 22 was be VBD cord-312431-de7zhswl 145 23 present present JJ cord-312431-de7zhswl 145 24 in in IN cord-312431-de7zhswl 145 25 substantial substantial JJ cord-312431-de7zhswl 145 26 ( ( -LRB- cord-312431-de7zhswl 145 27 20 20 CD cord-312431-de7zhswl 145 28 % % NN cord-312431-de7zhswl 145 29 ) ) -RRB- cord-312431-de7zhswl 145 30 numbers number NNS cord-312431-de7zhswl 145 31 . . . cord-312431-de7zhswl 146 1 The the DT cord-312431-de7zhswl 146 2 phages phage NNS cord-312431-de7zhswl 146 3 tagged tag VBD cord-312431-de7zhswl 146 4 as as IN cord-312431-de7zhswl 146 5 A1 A1 NNP cord-312431-de7zhswl 146 6 / / , cord-312431-de7zhswl 146 7 B1 b1 NN cord-312431-de7zhswl 146 8 were be VBD cord-312431-de7zhswl 146 9 not not RB cord-312431-de7zhswl 146 10 conclusive conclusive JJ cord-312431-de7zhswl 146 11 in in IN cord-312431-de7zhswl 146 12 morphological morphological JJ cord-312431-de7zhswl 146 13 appearance appearance NN cord-312431-de7zhswl 146 14 and and CC cord-312431-de7zhswl 146 15 could could MD cord-312431-de7zhswl 146 16 not not RB cord-312431-de7zhswl 146 17 be be VB cord-312431-de7zhswl 146 18 classified classify VBN cord-312431-de7zhswl 146 19 . . . cord-312431-de7zhswl 147 1 Table table NN cord-312431-de7zhswl 147 2 2 2 CD cord-312431-de7zhswl 147 3 illustrates illustrate VBZ cord-312431-de7zhswl 147 4 the the DT cord-312431-de7zhswl 147 5 size size NN cord-312431-de7zhswl 147 6 range range NN cord-312431-de7zhswl 147 7 of of IN cord-312431-de7zhswl 147 8 the the DT cord-312431-de7zhswl 147 9 bacteriophages bacteriophage NNS cord-312431-de7zhswl 147 10 that that WDT cord-312431-de7zhswl 147 11 could could MD cord-312431-de7zhswl 147 12 be be VB cord-312431-de7zhswl 147 13 observed observe VBN cord-312431-de7zhswl 147 14 during during IN cord-312431-de7zhswl 147 15 all all DT cord-312431-de7zhswl 147 16 four four CD cord-312431-de7zhswl 147 17 seasons season NNS cord-312431-de7zhswl 147 18 . . . cord-312431-de7zhswl 148 1 Phage phage NN cord-312431-de7zhswl 148 2 head head NN cord-312431-de7zhswl 148 3 diameters diameter NNS cord-312431-de7zhswl 148 4 Interesting interesting JJ cord-312431-de7zhswl 148 5 to to TO cord-312431-de7zhswl 148 6 note note VB cord-312431-de7zhswl 148 7 was be VBD cord-312431-de7zhswl 148 8 that that IN cord-312431-de7zhswl 148 9 the the DT cord-312431-de7zhswl 148 10 total total JJ cord-312431-de7zhswl 148 11 length length NN cord-312431-de7zhswl 148 12 of of IN cord-312431-de7zhswl 148 13 phages phage NNS cord-312431-de7zhswl 148 14 varied vary VBN cord-312431-de7zhswl 148 15 extensively extensively RB cord-312431-de7zhswl 148 16 , , , cord-312431-de7zhswl 148 17 ranging range VBG cord-312431-de7zhswl 148 18 from from IN cord-312431-de7zhswl 148 19 150 150 CD cord-312431-de7zhswl 148 20 nm nm NN cord-312431-de7zhswl 148 21 ( ( -LRB- cord-312431-de7zhswl 148 22 autumn autumn NN cord-312431-de7zhswl 148 23 ) ) -RRB- cord-312431-de7zhswl 148 24 , , , cord-312431-de7zhswl 148 25 181 181 CD cord-312431-de7zhswl 148 26 nm nm NN cord-312431-de7zhswl 148 27 ( ( -LRB- cord-312431-de7zhswl 148 28 winter winter NN cord-312431-de7zhswl 148 29 ) ) -RRB- cord-312431-de7zhswl 148 30 , , , cord-312431-de7zhswl 148 31 290 290 CD cord-312431-de7zhswl 148 32 nm nm CD cord-312431-de7zhswl 148 33 ( ( -LRB- cord-312431-de7zhswl 148 34 spring spring NN cord-312431-de7zhswl 148 35 ) ) -RRB- cord-312431-de7zhswl 148 36 to to IN cord-312431-de7zhswl 148 37 218 218 CD cord-312431-de7zhswl 148 38 nm nm NN cord-312431-de7zhswl 148 39 ( ( -LRB- cord-312431-de7zhswl 148 40 summer summer NN cord-312431-de7zhswl 148 41 ) ) -RRB- cord-312431-de7zhswl 148 42 . . . cord-312431-de7zhswl 149 1 Figures figure NNS cord-312431-de7zhswl 149 2 6 6 CD cord-312431-de7zhswl 149 3 and and CC cord-312431-de7zhswl 149 4 7 7 CD cord-312431-de7zhswl 149 5 resemble resemble JJ cord-312431-de7zhswl 149 6 bacteriophages bacteriophage NNS cord-312431-de7zhswl 149 7 detected detect VBN cord-312431-de7zhswl 149 8 in in IN cord-312431-de7zhswl 149 9 the the DT cord-312431-de7zhswl 149 10 Umgeni Umgeni NNP cord-312431-de7zhswl 149 11 River River NNP cord-312431-de7zhswl 149 12 water water NN cord-312431-de7zhswl 149 13 samples sample NNS cord-312431-de7zhswl 149 14 . . . cord-312431-de7zhswl 150 1 Phage phage NN cord-312431-de7zhswl 150 2 heads head NNS cord-312431-de7zhswl 150 3 examined examine VBN cord-312431-de7zhswl 150 4 , , , cord-312431-de7zhswl 150 5 appeared appear VBD cord-312431-de7zhswl 150 6 to to TO cord-312431-de7zhswl 150 7 be be VB cord-312431-de7zhswl 150 8 hexagonal hexagonal JJ cord-312431-de7zhswl 150 9 in in IN cord-312431-de7zhswl 150 10 outline outline NN cord-312431-de7zhswl 150 11 , , , cord-312431-de7zhswl 150 12 with with IN cord-312431-de7zhswl 150 13 six six CD cord-312431-de7zhswl 150 14 sided sided JJ cord-312431-de7zhswl 150 15 profiles profile NNS cord-312431-de7zhswl 150 16 which which WDT cord-312431-de7zhswl 150 17 were be VBD cord-312431-de7zhswl 150 18 regular regular JJ cord-312431-de7zhswl 150 19 , , , cord-312431-de7zhswl 150 20 with with IN cord-312431-de7zhswl 150 21 three three CD cord-312431-de7zhswl 150 22 symmetrical symmetrical JJ cord-312431-de7zhswl 150 23 axes axis NNS cord-312431-de7zhswl 150 24 ( ( -LRB- cord-312431-de7zhswl 150 25 e.g. e.g. DT cord-312431-de7zhswl 150 26 Fig Fig NNP cord-312431-de7zhswl 150 27 . . . cord-312431-de7zhswl 151 1 6a 6a NNP cord-312431-de7zhswl 151 2 , , , cord-312431-de7zhswl 151 3 b b NNP cord-312431-de7zhswl 151 4 , , , cord-312431-de7zhswl 151 5 c c NNP cord-312431-de7zhswl 151 6 , , , cord-312431-de7zhswl 151 7 e e NNP cord-312431-de7zhswl 151 8 and and CC cord-312431-de7zhswl 151 9 f f LS cord-312431-de7zhswl 151 10 ) ) -RRB- cord-312431-de7zhswl 151 11 while while IN cord-312431-de7zhswl 151 12 others other NNS cord-312431-de7zhswl 151 13 were be VBD cord-312431-de7zhswl 151 14 irregular irregular JJ cord-312431-de7zhswl 151 15 , , , cord-312431-de7zhswl 151 16 with with IN cord-312431-de7zhswl 151 17 only only RB cord-312431-de7zhswl 151 18 one one CD cord-312431-de7zhswl 151 19 symmetrical symmetrical JJ cord-312431-de7zhswl 151 20 axis axis NN cord-312431-de7zhswl 151 21 ( ( -LRB- cord-312431-de7zhswl 151 22 e.g. e.g. RB cord-312431-de7zhswl 151 23 Fig Fig NNP cord-312431-de7zhswl 151 24 . . . cord-312431-de7zhswl 152 1 6i 6i NNP cord-312431-de7zhswl 152 2 and and CC cord-312431-de7zhswl 152 3 k k NNP cord-312431-de7zhswl 152 4 ) ) -RRB- cord-312431-de7zhswl 152 5 . . . cord-312431-de7zhswl 153 1 However however RB cord-312431-de7zhswl 153 2 , , , cord-312431-de7zhswl 153 3 these these DT cord-312431-de7zhswl 153 4 data datum NNS cord-312431-de7zhswl 153 5 were be VBD cord-312431-de7zhswl 153 6 not not RB cord-312431-de7zhswl 153 7 adequate adequate JJ cord-312431-de7zhswl 153 8 to to TO cord-312431-de7zhswl 153 9 differentiate differentiate VB cord-312431-de7zhswl 153 10 between between IN cord-312431-de7zhswl 153 11 icosahedra icosahedron NNS cord-312431-de7zhswl 153 12 , , , cord-312431-de7zhswl 153 13 octahedra octahedra NN cord-312431-de7zhswl 153 14 and and CC cord-312431-de7zhswl 153 15 dodecahedra dodecahedra JJ cord-312431-de7zhswl 153 16 classification classification NN cord-312431-de7zhswl 153 17 . . . cord-312431-de7zhswl 154 1 Figure figure NN cord-312431-de7zhswl 154 2 6c 6c NNS cord-312431-de7zhswl 154 3 and and CC cord-312431-de7zhswl 154 4 h h NN cord-312431-de7zhswl 154 5 closely closely RB cord-312431-de7zhswl 154 6 resemble resemble JJ cord-312431-de7zhswl 154 7 members member NNS cord-312431-de7zhswl 154 8 of of IN cord-312431-de7zhswl 154 9 the the DT cord-312431-de7zhswl 154 10 family family NN cord-312431-de7zhswl 154 11 Myoviridae Myoviridae NNP cord-312431-de7zhswl 154 12 and and CC cord-312431-de7zhswl 154 13 Podoviridae Podoviridae NNP cord-312431-de7zhswl 154 14 , , , cord-312431-de7zhswl 154 15 respectively respectively RB cord-312431-de7zhswl 154 16 . . . cord-312431-de7zhswl 155 1 Figure figure NN cord-312431-de7zhswl 155 2 6l 6l CD cord-312431-de7zhswl 155 3 and and CC cord-312431-de7zhswl 155 4 p p LS cord-312431-de7zhswl 155 5 resemble resemble JJ cord-312431-de7zhswl 155 6 members member NNS cord-312431-de7zhswl 155 7 of of IN cord-312431-de7zhswl 155 8 the the DT cord-312431-de7zhswl 155 9 family family NN cord-312431-de7zhswl 155 10 B2 b2 NN cord-312431-de7zhswl 155 11 Siphoviridae Siphoviridae NNP cord-312431-de7zhswl 155 12 and and CC cord-312431-de7zhswl 155 13 B1 B1 NNP cord-312431-de7zhswl 155 14 Siphoviridae Siphoviridae NNP cord-312431-de7zhswl 155 15 , , , cord-312431-de7zhswl 155 16 respectively respectively RB cord-312431-de7zhswl 155 17 . . . cord-312431-de7zhswl 156 1 Figure figure NN cord-312431-de7zhswl 156 2 7 7 CD cord-312431-de7zhswl 156 3 represents represent VBZ cord-312431-de7zhswl 156 4 phage phage NN cord-312431-de7zhswl 156 5 particles particle NNS cord-312431-de7zhswl 156 6 detected detect VBN cord-312431-de7zhswl 156 7 in in IN cord-312431-de7zhswl 156 8 the the DT cord-312431-de7zhswl 156 9 Umgeni Umgeni NNP cord-312431-de7zhswl 156 10 River River NNP cord-312431-de7zhswl 156 11 at at IN cord-312431-de7zhswl 156 12 sites site NNS cord-312431-de7zhswl 156 13 U1 U1 NNP cord-312431-de7zhswl 156 14 , , , cord-312431-de7zhswl 156 15 U2 U2 NNP cord-312431-de7zhswl 156 16 and and CC cord-312431-de7zhswl 156 17 U3 U3 NNP cord-312431-de7zhswl 156 18 during during IN cord-312431-de7zhswl 156 19 spring spring NN cord-312431-de7zhswl 156 20 and and CC cord-312431-de7zhswl 156 21 summer summer NN cord-312431-de7zhswl 156 22 seasons season NNS cord-312431-de7zhswl 156 23 that that WDT cord-312431-de7zhswl 156 24 resemble resemble VBP cord-312431-de7zhswl 156 25 known know VBN cord-312431-de7zhswl 156 26 bacteriophages bacteriophage NNS cord-312431-de7zhswl 156 27 found find VBN cord-312431-de7zhswl 156 28 in in IN cord-312431-de7zhswl 156 29 literature literature NN cord-312431-de7zhswl 156 30 . . . cord-312431-de7zhswl 157 1 These these DT cord-312431-de7zhswl 157 2 include include VBP cord-312431-de7zhswl 157 3 known know VBN cord-312431-de7zhswl 157 4 phages phage NNS cord-312431-de7zhswl 157 5 of of IN cord-312431-de7zhswl 157 6 : : : cord-312431-de7zhswl 157 7 71A-6 71a-6 CD cord-312431-de7zhswl 157 8 of of IN cord-312431-de7zhswl 157 9 Vibrio Vibrio NNP cord-312431-de7zhswl 157 10 vulnificus vulnificus NNP cord-312431-de7zhswl 157 11 phage phage NN cord-312431-de7zhswl 157 12 , , , cord-312431-de7zhswl 157 13 phage phage NN cord-312431-de7zhswl 157 14 T4 T4 NNP cord-312431-de7zhswl 157 15 and and CC cord-312431-de7zhswl 157 16 T4-like t4-like IN cord-312431-de7zhswl 157 17 Vibrio Vibrio NNP cord-312431-de7zhswl 157 18 parahaemolyticus parahaemolyticus NN cord-312431-de7zhswl 157 19 phage phage NN cord-312431-de7zhswl 157 20 as as IN cord-312431-de7zhswl 157 21 shown show VBN cord-312431-de7zhswl 157 22 by by IN cord-312431-de7zhswl 157 23 Ackermann Ackermann NNP cord-312431-de7zhswl 157 24 and and CC cord-312431-de7zhswl 157 25 Heldal Heldal NNP cord-312431-de7zhswl 157 26 [ [ -LRB- cord-312431-de7zhswl 157 27 50 50 CD cord-312431-de7zhswl 157 28 ] ] -RRB- cord-312431-de7zhswl 157 29 . . . cord-312431-de7zhswl 158 1 Figures figure NNS cord-312431-de7zhswl 158 2 8 8 CD cord-312431-de7zhswl 158 3 - - SYM cord-312431-de7zhswl 158 4 12 12 CD cord-312431-de7zhswl 158 5 illustrate illustrate VBP cord-312431-de7zhswl 158 6 several several JJ cord-312431-de7zhswl 158 7 presumptive presumptive JJ cord-312431-de7zhswl 158 8 virus virus NN cord-312431-de7zhswl 158 9 types type NNS cord-312431-de7zhswl 158 10 that that WDT cord-312431-de7zhswl 158 11 were be VBD cord-312431-de7zhswl 158 12 found find VBN cord-312431-de7zhswl 158 13 in in IN cord-312431-de7zhswl 158 14 the the DT cord-312431-de7zhswl 158 15 Umgeni Umgeni NNP cord-312431-de7zhswl 158 16 River River NNP cord-312431-de7zhswl 158 17 during during IN cord-312431-de7zhswl 158 18 all all DT cord-312431-de7zhswl 158 19 seasons season NNS cord-312431-de7zhswl 158 20 . . . cord-312431-de7zhswl 159 1 These these DT cord-312431-de7zhswl 159 2 presumptive presumptive JJ cord-312431-de7zhswl 159 3 viruses virus NNS cord-312431-de7zhswl 159 4 were be VBD cord-312431-de7zhswl 159 5 compared compare VBN cord-312431-de7zhswl 159 6 to to IN cord-312431-de7zhswl 159 7 their -PRON- PRP$ cord-312431-de7zhswl 159 8 known know VBN cord-312431-de7zhswl 159 9 structures structure NNS cord-312431-de7zhswl 159 10 illustrated illustrate VBN cord-312431-de7zhswl 159 11 in in IN cord-312431-de7zhswl 159 12 literature literature NN cord-312431-de7zhswl 159 13 . . . cord-312431-de7zhswl 160 1 Presumptive presumptive JJ cord-312431-de7zhswl 160 2 naked naked JJ cord-312431-de7zhswl 160 3 Adenoviridae Adenoviridae NNP cord-312431-de7zhswl 160 4 - - HYPH cord-312431-de7zhswl 160 5 like like JJ cord-312431-de7zhswl 160 6 particles particle NNS cord-312431-de7zhswl 160 7 ranging range VBG cord-312431-de7zhswl 160 8 in in IN cord-312431-de7zhswl 160 9 size size NN cord-312431-de7zhswl 160 10 from from IN cord-312431-de7zhswl 160 11 66.97 66.97 CD cord-312431-de7zhswl 160 12 to to IN cord-312431-de7zhswl 160 13 74.40 74.40 CD cord-312431-de7zhswl 160 14 nm nm NN cord-312431-de7zhswl 160 15 are be VBP cord-312431-de7zhswl 160 16 shown show VBN cord-312431-de7zhswl 160 17 in in IN cord-312431-de7zhswl 160 18 Fig Fig NNP cord-312431-de7zhswl 160 19 . . . cord-312431-de7zhswl 161 1 8 8 CD cord-312431-de7zhswl 162 1 and and CC cord-312431-de7zhswl 162 2 these these DT cord-312431-de7zhswl 162 3 images image NNS cord-312431-de7zhswl 162 4 are be VBP cord-312431-de7zhswl 162 5 compared compare VBN cord-312431-de7zhswl 162 6 to to IN cord-312431-de7zhswl 162 7 known know VBN cord-312431-de7zhswl 162 8 adenoviruses adenovirus NNS cord-312431-de7zhswl 162 9 ( ( -LRB- cord-312431-de7zhswl 162 10 70 70 CD cord-312431-de7zhswl 162 11 - - SYM cord-312431-de7zhswl 162 12 90 90 CD cord-312431-de7zhswl 162 13 nm nm NN cord-312431-de7zhswl 162 14 ) ) -RRB- cord-312431-de7zhswl 162 15 ( ( -LRB- cord-312431-de7zhswl 162 16 Fig Fig NNP cord-312431-de7zhswl 162 17 . . . cord-312431-de7zhswl 162 18 8 8 CD cord-312431-de7zhswl 162 19 g g NN cord-312431-de7zhswl 162 20 ) ) -RRB- cord-312431-de7zhswl 162 21 . . . cord-312431-de7zhswl 163 1 Figure figure NN cord-312431-de7zhswl 163 2 9 9 CD cord-312431-de7zhswl 163 3 represents represent VBZ cord-312431-de7zhswl 163 4 TEM tem NN cord-312431-de7zhswl 163 5 images image NNS cord-312431-de7zhswl 163 6 of of IN cord-312431-de7zhswl 163 7 presumptive presumptive JJ cord-312431-de7zhswl 163 8 naked naked JJ cord-312431-de7zhswl 163 9 Picornaviridae Picornaviridae NNP cord-312431-de7zhswl 163 10 ( ( -LRB- cord-312431-de7zhswl 163 11 enterovirus)-like enterovirus)-like NNP cord-312431-de7zhswl 163 12 particles particle NNS cord-312431-de7zhswl 163 13 ranging range VBG cord-312431-de7zhswl 163 14 in in IN cord-312431-de7zhswl 163 15 size size NN cord-312431-de7zhswl 163 16 from from IN cord-312431-de7zhswl 163 17 26 26 CD cord-312431-de7zhswl 163 18 to to TO cord-312431-de7zhswl 163 19 30 30 CD cord-312431-de7zhswl 163 20 nm nm NN cord-312431-de7zhswl 163 21 , , , cord-312431-de7zhswl 163 22 compared compare VBN cord-312431-de7zhswl 163 23 to to IN cord-312431-de7zhswl 163 24 a a DT cord-312431-de7zhswl 163 25 known know VBN cord-312431-de7zhswl 163 26 coxsackievirus coxsackievirus NN cord-312431-de7zhswl 163 27 ( ( -LRB- cord-312431-de7zhswl 163 28 Fig Fig NNP cord-312431-de7zhswl 163 29 . . . cord-312431-de7zhswl 163 30 9f 9f CD cord-312431-de7zhswl 163 31 ) ) -RRB- cord-312431-de7zhswl 163 32 . . . cord-312431-de7zhswl 164 1 Poxviridae Poxviridae NNP cord-312431-de7zhswl 164 2 - - HYPH cord-312431-de7zhswl 164 3 like like JJ cord-312431-de7zhswl 164 4 particles particle NNS cord-312431-de7zhswl 164 5 ( ( -LRB- cord-312431-de7zhswl 164 6 Fig Fig NNP cord-312431-de7zhswl 164 7 . . NNP cord-312431-de7zhswl 164 8 10a 10a CD cord-312431-de7zhswl 164 9 - - HYPH cord-312431-de7zhswl 164 10 c c NN cord-312431-de7zhswl 164 11 ) ) -RRB- cord-312431-de7zhswl 164 12 and and CC cord-312431-de7zhswl 164 13 Herpesviridae Herpesviridae NNP cord-312431-de7zhswl 164 14 - - HYPH cord-312431-de7zhswl 164 15 like like JJ cord-312431-de7zhswl 164 16 particles particle NNS cord-312431-de7zhswl 164 17 ( ( -LRB- cord-312431-de7zhswl 164 18 Fig Fig NNP cord-312431-de7zhswl 164 19 . . NNP cord-312431-de7zhswl 164 20 10e 10e , cord-312431-de7zhswl 164 21 - - : cord-312431-de7zhswl 164 22 g g NN cord-312431-de7zhswl 164 23 ) ) -RRB- cord-312431-de7zhswl 164 24 were be VBD cord-312431-de7zhswl 164 25 also also RB cord-312431-de7zhswl 164 26 detected detect VBN cord-312431-de7zhswl 164 27 in in IN cord-312431-de7zhswl 164 28 the the DT cord-312431-de7zhswl 164 29 Umgeni Umgeni NNP cord-312431-de7zhswl 164 30 River River NNP cord-312431-de7zhswl 164 31 water water NN cord-312431-de7zhswl 164 32 samples sample NNS cord-312431-de7zhswl 164 33 and and CC cord-312431-de7zhswl 164 34 these these DT cord-312431-de7zhswl 164 35 were be VBD cord-312431-de7zhswl 164 36 found find VBN cord-312431-de7zhswl 164 37 to to TO cord-312431-de7zhswl 164 38 be be VB cord-312431-de7zhswl 164 39 similar similar JJ cord-312431-de7zhswl 164 40 in in IN cord-312431-de7zhswl 164 41 literature literature NN cord-312431-de7zhswl 164 42 to to IN cord-312431-de7zhswl 164 43 known know VBN cord-312431-de7zhswl 164 44 poxvirus poxvirus NNP cord-312431-de7zhswl 164 45 ( ( -LRB- cord-312431-de7zhswl 164 46 Fig Fig NNP cord-312431-de7zhswl 164 47 . . . cord-312431-de7zhswl 164 48 10d 10d CD cord-312431-de7zhswl 164 49 ) ) -RRB- cord-312431-de7zhswl 164 50 and and CC cord-312431-de7zhswl 164 51 known know VBN cord-312431-de7zhswl 164 52 herpesvirus herpesvirus NN cord-312431-de7zhswl 164 53 ( ( -LRB- cord-312431-de7zhswl 164 54 Fig Fig NNP cord-312431-de7zhswl 164 55 . . NNP cord-312431-de7zhswl 164 56 10h 10h CD cord-312431-de7zhswl 164 57 ) ) -RRB- cord-312431-de7zhswl 164 58 . . . cord-312431-de7zhswl 165 1 Figure figure NN cord-312431-de7zhswl 165 2 11 11 CD cord-312431-de7zhswl 165 3 illustrates illustrate VBZ cord-312431-de7zhswl 165 4 presumptive presumptive JJ cord-312431-de7zhswl 165 5 Reoviridae Reoviridae NNP cord-312431-de7zhswl 165 6 - - HYPH cord-312431-de7zhswl 165 7 like like JJ cord-312431-de7zhswl 165 8 particles particle NNS cord-312431-de7zhswl 165 9 ranging range VBG cord-312431-de7zhswl 165 10 in in IN cord-312431-de7zhswl 165 11 size size NN cord-312431-de7zhswl 165 12 from from IN cord-312431-de7zhswl 165 13 18 18 CD cord-312431-de7zhswl 165 14 to to TO cord-312431-de7zhswl 165 15 20 20 CD cord-312431-de7zhswl 165 16 nm nm NN cord-312431-de7zhswl 166 1 ( ( -LRB- cord-312431-de7zhswl 166 2 Fig Fig NNP cord-312431-de7zhswl 166 3 . . NNP cord-312431-de7zhswl 166 4 11a 11a NNS cord-312431-de7zhswl 166 5 - - HYPH cord-312431-de7zhswl 166 6 c c NN cord-312431-de7zhswl 166 7 ) ) -RRB- cord-312431-de7zhswl 167 1 compared compare VBN cord-312431-de7zhswl 167 2 to to IN cord-312431-de7zhswl 167 3 a a DT cord-312431-de7zhswl 167 4 known know VBN cord-312431-de7zhswl 167 5 rotavirus rotavirus NN cord-312431-de7zhswl 167 6 ( ( -LRB- cord-312431-de7zhswl 167 7 20 20 CD cord-312431-de7zhswl 167 8 - - SYM cord-312431-de7zhswl 167 9 30 30 CD cord-312431-de7zhswl 167 10 nm nm NN cord-312431-de7zhswl 167 11 ) ) -RRB- cord-312431-de7zhswl 167 12 ( ( -LRB- cord-312431-de7zhswl 167 13 Fig Fig NNP cord-312431-de7zhswl 167 14 . . . cord-312431-de7zhswl 167 15 11d 11d CD cord-312431-de7zhswl 167 16 ) ) -RRB- cord-312431-de7zhswl 167 17 . . . cord-312431-de7zhswl 168 1 Presumptive presumptive JJ cord-312431-de7zhswl 168 2 Caliciviridae Caliciviridae NNP cord-312431-de7zhswl 168 3 - - HYPH cord-312431-de7zhswl 168 4 like like JJ cord-312431-de7zhswl 168 5 particles particle NNS cord-312431-de7zhswl 168 6 are be VBP cord-312431-de7zhswl 168 7 illustrated illustrate VBN cord-312431-de7zhswl 168 8 in in IN cord-312431-de7zhswl 168 9 Fig Fig NNP cord-312431-de7zhswl 168 10 . . . cord-312431-de7zhswl 169 1 11e 11e . cord-312431-de7zhswl 170 1 -g -g . cord-312431-de7zhswl 171 1 and and CC cord-312431-de7zhswl 171 2 these these DT cord-312431-de7zhswl 171 3 had have VBD cord-312431-de7zhswl 171 4 a a DT cord-312431-de7zhswl 171 5 size size NN cord-312431-de7zhswl 171 6 of of IN cord-312431-de7zhswl 171 7 35 35 CD cord-312431-de7zhswl 171 8 nm nm NN cord-312431-de7zhswl 171 9 , , , cord-312431-de7zhswl 171 10 similar similar JJ cord-312431-de7zhswl 171 11 to to IN cord-312431-de7zhswl 171 12 that that DT cord-312431-de7zhswl 171 13 of of IN cord-312431-de7zhswl 171 14 a a DT cord-312431-de7zhswl 171 15 known know VBN cord-312431-de7zhswl 171 16 norovirus norovirus NN cord-312431-de7zhswl 171 17 ( ( -LRB- cord-312431-de7zhswl 171 18 Fig Fig NNP cord-312431-de7zhswl 171 19 . . . cord-312431-de7zhswl 171 20 11h 11h CD cord-312431-de7zhswl 171 21 ) ) -RRB- cord-312431-de7zhswl 171 22 of of IN cord-312431-de7zhswl 171 23 30 30 CD cord-312431-de7zhswl 171 24 - - SYM cord-312431-de7zhswl 171 25 40 40 CD cord-312431-de7zhswl 171 26 nm nm NN cord-312431-de7zhswl 171 27 . . . cord-312431-de7zhswl 172 1 Presumptive presumptive NN cord-312431-de7zhswl 172 2 enveloped envelop VBN cord-312431-de7zhswl 172 3 Coronaviridae Coronaviridae NNP cord-312431-de7zhswl 172 4 - - HYPH cord-312431-de7zhswl 172 5 like like JJ cord-312431-de7zhswl 172 6 particles particle NNS cord-312431-de7zhswl 172 7 ( ( -LRB- cord-312431-de7zhswl 172 8 Fig Fig NNP cord-312431-de7zhswl 172 9 . . NNP cord-312431-de7zhswl 172 10 12a 12a NNS cord-312431-de7zhswl 172 11 - - HYPH cord-312431-de7zhswl 172 12 c c NN cord-312431-de7zhswl 172 13 ) ) -RRB- cord-312431-de7zhswl 172 14 were be VBD cord-312431-de7zhswl 172 15 found find VBN cord-312431-de7zhswl 172 16 to to TO cord-312431-de7zhswl 172 17 be be VB cord-312431-de7zhswl 172 18 similar similar JJ cord-312431-de7zhswl 172 19 to to IN cord-312431-de7zhswl 172 20 a a DT cord-312431-de7zhswl 172 21 known know VBN cord-312431-de7zhswl 172 22 coronavirus coronavirus NN cord-312431-de7zhswl 172 23 ( ( -LRB- cord-312431-de7zhswl 172 24 Fig Fig NNP cord-312431-de7zhswl 172 25 . . . cord-312431-de7zhswl 172 26 12d 12d CD cord-312431-de7zhswl 172 27 ) ) -RRB- cord-312431-de7zhswl 172 28 , , , cord-312431-de7zhswl 172 29 with with IN cord-312431-de7zhswl 172 30 presumptive presumptive JJ cord-312431-de7zhswl 172 31 Orthomyxoviridae Orthomyxoviridae NNP cord-312431-de7zhswl 172 32 - - HYPH cord-312431-de7zhswl 172 33 like like JJ cord-312431-de7zhswl 172 34 particles particle NNS cord-312431-de7zhswl 172 35 being be VBG cord-312431-de7zhswl 172 36 similar similar JJ cord-312431-de7zhswl 172 37 to to IN cord-312431-de7zhswl 172 38 a a DT cord-312431-de7zhswl 172 39 known know VBN cord-312431-de7zhswl 172 40 influenza influenza NN cord-312431-de7zhswl 172 41 virus virus NN cord-312431-de7zhswl 172 42 ( ( -LRB- cord-312431-de7zhswl 172 43 Fig Fig NNP cord-312431-de7zhswl 172 44 . . . cord-312431-de7zhswl 172 45 12h 12h CD cord-312431-de7zhswl 172 46 ) ) -RRB- cord-312431-de7zhswl 172 47 . . . cord-312431-de7zhswl 173 1 The the DT cord-312431-de7zhswl 173 2 CPE CPE NNP cord-312431-de7zhswl 173 3 is be VBZ cord-312431-de7zhswl 173 4 an an DT cord-312431-de7zhswl 173 5 observable observable JJ cord-312431-de7zhswl 173 6 morphological morphological JJ cord-312431-de7zhswl 173 7 ( ( -LRB- cord-312431-de7zhswl 173 8 shape shape NN cord-312431-de7zhswl 173 9 ) ) -RRB- cord-312431-de7zhswl 173 10 change change NN cord-312431-de7zhswl 173 11 in in IN cord-312431-de7zhswl 173 12 tissue tissue NN cord-312431-de7zhswl 173 13 cells cell NNS cord-312431-de7zhswl 173 14 due due JJ cord-312431-de7zhswl 173 15 to to IN cord-312431-de7zhswl 173 16 viral viral JJ cord-312431-de7zhswl 173 17 infection infection NN cord-312431-de7zhswl 173 18 . . . cord-312431-de7zhswl 174 1 Figure figure NN cord-312431-de7zhswl 174 2 13 13 CD cord-312431-de7zhswl 174 3 illustrates illustrate VBZ cord-312431-de7zhswl 174 4 the the DT cord-312431-de7zhswl 174 5 results result NNS cord-312431-de7zhswl 174 6 obtained obtain VBN cord-312431-de7zhswl 174 7 for for IN cord-312431-de7zhswl 174 8 the the DT cord-312431-de7zhswl 174 9 Figure Figure NNP cord-312431-de7zhswl 174 10 7 7 CD cord-312431-de7zhswl 174 11 . . . cord-312431-de7zhswl 175 1 TEM tem NN cord-312431-de7zhswl 175 2 images image NNS cord-312431-de7zhswl 175 3 of of IN cord-312431-de7zhswl 175 4 phage phage NN cord-312431-de7zhswl 175 5 morphotypes morphotype NNS cord-312431-de7zhswl 175 6 ( ( -LRB- cord-312431-de7zhswl 175 7 a a NNP cord-312431-de7zhswl 175 8 , , , cord-312431-de7zhswl 175 9 c c NNP cord-312431-de7zhswl 175 10 , , , cord-312431-de7zhswl 175 11 e e LS cord-312431-de7zhswl 175 12 ) ) -RRB- cord-312431-de7zhswl 175 13 present present JJ cord-312431-de7zhswl 175 14 downstream downstream NN cord-312431-de7zhswl 175 15 of of IN cord-312431-de7zhswl 175 16 the the DT cord-312431-de7zhswl 175 17 Umgeni Umgeni NNP cord-312431-de7zhswl 175 18 River River NNP cord-312431-de7zhswl 175 19 at at IN cord-312431-de7zhswl 175 20 the the DT cord-312431-de7zhswl 175 21 sampling sampling NN cord-312431-de7zhswl 175 22 sites site NNS cord-312431-de7zhswl 175 23 U1 U1 NNP cord-312431-de7zhswl 175 24 , , , cord-312431-de7zhswl 175 25 U2 U2 NNP cord-312431-de7zhswl 175 26 and and CC cord-312431-de7zhswl 175 27 U3 U3 NNP cord-312431-de7zhswl 175 28 , , , cord-312431-de7zhswl 175 29 respectively respectively RB cord-312431-de7zhswl 175 30 , , , cord-312431-de7zhswl 175 31 during during IN cord-312431-de7zhswl 175 32 spring spring NN cord-312431-de7zhswl 175 33 and and CC cord-312431-de7zhswl 175 34 summer summer NN cord-312431-de7zhswl 175 35 , , , cord-312431-de7zhswl 175 36 ( ( -LRB- cord-312431-de7zhswl 175 37 b b NN cord-312431-de7zhswl 175 38 ) ) -RRB- cord-312431-de7zhswl 175 39 known know VBN cord-312431-de7zhswl 175 40 71A-6 71a-6 CD cord-312431-de7zhswl 175 41 of of IN cord-312431-de7zhswl 175 42 Vibrio Vibrio NNP cord-312431-de7zhswl 175 43 vulnificus vulnificus NNP cord-312431-de7zhswl 175 44 phage phage NN cord-312431-de7zhswl 175 45 , , , cord-312431-de7zhswl 175 46 ( ( -LRB- cord-312431-de7zhswl 175 47 d d NN cord-312431-de7zhswl 175 48 ) ) -RRB- cord-312431-de7zhswl 175 49 known know VBN cord-312431-de7zhswl 175 50 phage phage NN cord-312431-de7zhswl 175 51 T4 T4 NNP cord-312431-de7zhswl 175 52 , , , cord-312431-de7zhswl 175 53 ( ( -LRB- cord-312431-de7zhswl 175 54 f f NNP cord-312431-de7zhswl 175 55 ) ) -RRB- cord-312431-de7zhswl 175 56 known know VBN cord-312431-de7zhswl 175 57 T4-like t4-like IN cord-312431-de7zhswl 175 58 Vibrio vibrio NN cord-312431-de7zhswl 175 59 parahaemolyticus parahaemolyticus NN cord-312431-de7zhswl 175 60 phage phage NN cord-312431-de7zhswl 175 61 [ [ -LRB- cord-312431-de7zhswl 175 62 50 50 CD cord-312431-de7zhswl 175 63 ] ] -RRB- cord-312431-de7zhswl 175 64 . . . cord-312431-de7zhswl 176 1 Images image NNS cord-312431-de7zhswl 176 2 captured capture VBN cord-312431-de7zhswl 176 3 at at IN cord-312431-de7zhswl 176 4 300 300 CD cord-312431-de7zhswl 176 5 000 000 CD cord-312431-de7zhswl 176 6 - - SYM cord-312431-de7zhswl 176 7 400 400 CD cord-312431-de7zhswl 176 8 000Â 000â CD cord-312431-de7zhswl 176 9 magnification magnification NN cord-312431-de7zhswl 176 10 . . . cord-312431-de7zhswl 177 1 Scale scale NN cord-312431-de7zhswl 177 2 bar bar NN cord-312431-de7zhswl 177 3 : : : cord-312431-de7zhswl 177 4 100 100 CD cord-312431-de7zhswl 177 5 nm nm NN cord-312431-de7zhswl 177 6 Figure Figure NNP cord-312431-de7zhswl 177 7 8 8 CD cord-312431-de7zhswl 177 8 . . . cord-312431-de7zhswl 178 1 TEM tem NN cord-312431-de7zhswl 178 2 images image NNS cord-312431-de7zhswl 178 3 of of IN cord-312431-de7zhswl 178 4 presumptive presumptive JJ cord-312431-de7zhswl 178 5 naked naked JJ cord-312431-de7zhswl 178 6 Adenoviridae Adenoviridae NNP cord-312431-de7zhswl 178 7 - - HYPH cord-312431-de7zhswl 178 8 like like JJ cord-312431-de7zhswl 178 9 particles particle NNS cord-312431-de7zhswl 178 10 and and CC cord-312431-de7zhswl 178 11 ( ( -LRB- cord-312431-de7zhswl 178 12 g g NN cord-312431-de7zhswl 178 13 ) ) -RRB- cord-312431-de7zhswl 178 14 a a DT cord-312431-de7zhswl 178 15 known know VBN cord-312431-de7zhswl 178 16 adenovirus adenovirus NN cord-312431-de7zhswl 178 17 [ [ -LRB- cord-312431-de7zhswl 178 18 51 51 CD cord-312431-de7zhswl 178 19 ] ] -RRB- cord-312431-de7zhswl 178 20 , , , cord-312431-de7zhswl 178 21 present present JJ cord-312431-de7zhswl 178 22 in in IN cord-312431-de7zhswl 178 23 the the DT cord-312431-de7zhswl 178 24 Umgeni Umgeni NNP cord-312431-de7zhswl 178 25 River River NNP cord-312431-de7zhswl 178 26 at at IN cord-312431-de7zhswl 178 27 the the DT cord-312431-de7zhswl 178 28 sampling sampling NN cord-312431-de7zhswl 178 29 sites site NNS cord-312431-de7zhswl 178 30 U1 U1 NNP cord-312431-de7zhswl 178 31 , , , cord-312431-de7zhswl 178 32 U2 U2 NNP cord-312431-de7zhswl 178 33 , , , cord-312431-de7zhswl 178 34 U3 U3 NNP cord-312431-de7zhswl 178 35 , , , cord-312431-de7zhswl 178 36 U4 u4 NN cord-312431-de7zhswl 178 37 and and CC cord-312431-de7zhswl 178 38 U5 U5 NNP cord-312431-de7zhswl 178 39 during during IN cord-312431-de7zhswl 178 40 autumn autumn NN cord-312431-de7zhswl 178 41 , , , cord-312431-de7zhswl 178 42 spring spring NN cord-312431-de7zhswl 178 43 and and CC cord-312431-de7zhswl 178 44 summer summer NN cord-312431-de7zhswl 178 45 tested test VBN cord-312431-de7zhswl 178 46 . . . cord-312431-de7zhswl 179 1 Images image NNS cord-312431-de7zhswl 179 2 captured capture VBN cord-312431-de7zhswl 179 3 at at IN cord-312431-de7zhswl 179 4 400 400 CD cord-312431-de7zhswl 179 5 000 000 CD cord-312431-de7zhswl 179 6 - - HYPH cord-312431-de7zhswl 179 7 600 600 CD cord-312431-de7zhswl 179 8 000Â 000â CD cord-312431-de7zhswl 179 9 magnification magnification NN cord-312431-de7zhswl 179 10 . . . cord-312431-de7zhswl 180 1 Scale scale NN cord-312431-de7zhswl 180 2 bar bar NN cord-312431-de7zhswl 180 3 ; ; : cord-312431-de7zhswl 180 4 100 100 CD cord-312431-de7zhswl 180 5 nm nm NN cord-312431-de7zhswl 180 6 . . . cord-312431-de7zhswl 181 1 CPE CPE NNP cord-312431-de7zhswl 181 2 of of IN cord-312431-de7zhswl 181 3 the the DT cord-312431-de7zhswl 181 4 VLPs vlp NNS cord-312431-de7zhswl 181 5 isolated isolate VBN cord-312431-de7zhswl 181 6 from from IN cord-312431-de7zhswl 181 7 the the DT cord-312431-de7zhswl 181 8 Umgeni Umgeni NNP cord-312431-de7zhswl 181 9 River River NNP cord-312431-de7zhswl 181 10 using use VBG cord-312431-de7zhswl 181 11 six six CD cord-312431-de7zhswl 181 12 tissue tissue NN cord-312431-de7zhswl 181 13 cell cell NN cord-312431-de7zhswl 181 14 lines line NNS cord-312431-de7zhswl 181 15 . . . cord-312431-de7zhswl 182 1 The the DT cord-312431-de7zhswl 182 2 identification identification NN cord-312431-de7zhswl 182 3 of of IN cord-312431-de7zhswl 182 4 CPE CPE NNP cord-312431-de7zhswl 182 5 in in IN cord-312431-de7zhswl 182 6 the the DT cord-312431-de7zhswl 182 7 cell cell NN cord-312431-de7zhswl 182 8 cultures culture NNS cord-312431-de7zhswl 182 9 included include VBD cord-312431-de7zhswl 182 10 the the DT cord-312431-de7zhswl 182 11 occurrence occurrence NN cord-312431-de7zhswl 182 12 of of IN cord-312431-de7zhswl 182 13 elongated elongated JJ cord-312431-de7zhswl 182 14 , , , cord-312431-de7zhswl 182 15 granulated granulated JJ cord-312431-de7zhswl 182 16 cells cell NNS cord-312431-de7zhswl 182 17 , , , cord-312431-de7zhswl 182 18 loss loss NN cord-312431-de7zhswl 182 19 of of IN cord-312431-de7zhswl 182 20 cell cell NN cord-312431-de7zhswl 182 21 to to IN cord-312431-de7zhswl 182 22 cell cell NN cord-312431-de7zhswl 182 23 contact contact NN cord-312431-de7zhswl 182 24 and and CC cord-312431-de7zhswl 182 25 the the DT cord-312431-de7zhswl 182 26 appearance appearance NN cord-312431-de7zhswl 182 27 of of IN cord-312431-de7zhswl 182 28 vacuoles vacuole NNS cord-312431-de7zhswl 182 29 . . . cord-312431-de7zhswl 183 1 All all DT cord-312431-de7zhswl 183 2 VLPs vlp NNS cord-312431-de7zhswl 183 3 isolated isolate VBN cord-312431-de7zhswl 183 4 from from IN cord-312431-de7zhswl 183 5 all all DT cord-312431-de7zhswl 183 6 water water NN cord-312431-de7zhswl 183 7 samples sample NNS cord-312431-de7zhswl 183 8 during during IN cord-312431-de7zhswl 183 9 all all DT cord-312431-de7zhswl 183 10 four four CD cord-312431-de7zhswl 183 11 seasons season NNS cord-312431-de7zhswl 183 12 were be VBD cord-312431-de7zhswl 183 13 capable capable JJ cord-312431-de7zhswl 183 14 of of IN cord-312431-de7zhswl 183 15 inducing induce VBG cord-312431-de7zhswl 183 16 some some DT cord-312431-de7zhswl 183 17 CPE CPE NNP cord-312431-de7zhswl 183 18 ( ( -LRB- cord-312431-de7zhswl 183 19 cell cell NN cord-312431-de7zhswl 183 20 death death NN cord-312431-de7zhswl 183 21 ) ) -RRB- cord-312431-de7zhswl 183 22 on on IN cord-312431-de7zhswl 183 23 all all DT cord-312431-de7zhswl 183 24 tissue tissue NN cord-312431-de7zhswl 183 25 cell cell NN cord-312431-de7zhswl 183 26 lines line NNS cord-312431-de7zhswl 183 27 tested test VBN cord-312431-de7zhswl 183 28 . . . cord-312431-de7zhswl 184 1 The the DT cord-312431-de7zhswl 184 2 VLP VLP NNP cord-312431-de7zhswl 184 3 samples sample NNS cord-312431-de7zhswl 184 4 from from IN cord-312431-de7zhswl 184 5 sites site NNS cord-312431-de7zhswl 184 6 U3 u3 NN cord-312431-de7zhswl 184 7 , , , cord-312431-de7zhswl 184 8 U4 u4 NN cord-312431-de7zhswl 184 9 and and CC cord-312431-de7zhswl 184 10 U5 U5 NNP cord-312431-de7zhswl 184 11 produced produce VBD cord-312431-de7zhswl 184 12 no no DT cord-312431-de7zhswl 184 13 CPE CPE NNP cord-312431-de7zhswl 184 14 on on IN cord-312431-de7zhswl 184 15 the the DT cord-312431-de7zhswl 184 16 Hep Hep NNP cord-312431-de7zhswl 184 17 - - HYPH cord-312431-de7zhswl 184 18 G2 G2 NNP cord-312431-de7zhswl 184 19 cell cell NN cord-312431-de7zhswl 184 20 line line NN cord-312431-de7zhswl 184 21 during during IN cord-312431-de7zhswl 184 22 autumn autumn NN cord-312431-de7zhswl 184 23 . . . cord-312431-de7zhswl 185 1 VLPs vlp NNS cord-312431-de7zhswl 185 2 from from IN cord-312431-de7zhswl 185 3 site site NN cord-312431-de7zhswl 185 4 U5 U5 NNP cord-312431-de7zhswl 185 5 produced produce VBD cord-312431-de7zhswl 185 6 CPE CPE NNP cord-312431-de7zhswl 185 7 Figure Figure NNP cord-312431-de7zhswl 185 8 9 9 CD cord-312431-de7zhswl 185 9 . . . cord-312431-de7zhswl 186 1 TEM tem NN cord-312431-de7zhswl 186 2 images image NNS cord-312431-de7zhswl 186 3 of of IN cord-312431-de7zhswl 186 4 presumptive presumptive JJ cord-312431-de7zhswl 186 5 naked naked JJ cord-312431-de7zhswl 186 6 Picornaviridae Picornaviridae NNP cord-312431-de7zhswl 186 7 ( ( -LRB- cord-312431-de7zhswl 186 8 enterovirus)-like enterovirus)-like NNP cord-312431-de7zhswl 186 9 particles particle NNS cord-312431-de7zhswl 186 10 and and CC cord-312431-de7zhswl 186 11 ( ( -LRB- cord-312431-de7zhswl 186 12 f f LS cord-312431-de7zhswl 186 13 ) ) -RRB- cord-312431-de7zhswl 186 14 a a DT cord-312431-de7zhswl 186 15 known know VBN cord-312431-de7zhswl 186 16 coxsackievirus coxsackievirus NN cord-312431-de7zhswl 186 17 [ [ -LRB- cord-312431-de7zhswl 186 18 52 52 CD cord-312431-de7zhswl 186 19 ] ] -RRB- cord-312431-de7zhswl 186 20 , , , cord-312431-de7zhswl 186 21 present present JJ cord-312431-de7zhswl 186 22 in in IN cord-312431-de7zhswl 186 23 the the DT cord-312431-de7zhswl 186 24 Umgeni Umgeni NNP cord-312431-de7zhswl 186 25 River River NNP cord-312431-de7zhswl 186 26 at at IN cord-312431-de7zhswl 186 27 sampling sampling NN cord-312431-de7zhswl 186 28 sites site NNS cord-312431-de7zhswl 186 29 U1 U1 NNP cord-312431-de7zhswl 186 30 , , , cord-312431-de7zhswl 186 31 U2 U2 NNP cord-312431-de7zhswl 186 32 , , , cord-312431-de7zhswl 186 33 and and CC cord-312431-de7zhswl 186 34 U3 u3 NN cord-312431-de7zhswl 186 35 during during IN cord-312431-de7zhswl 186 36 spring spring NN cord-312431-de7zhswl 186 37 and and CC cord-312431-de7zhswl 186 38 summer summer NN cord-312431-de7zhswl 186 39 tested test VBN cord-312431-de7zhswl 186 40 . . . cord-312431-de7zhswl 187 1 Images image NNS cord-312431-de7zhswl 187 2 captured capture VBN cord-312431-de7zhswl 187 3 at at IN cord-312431-de7zhswl 187 4 500 500 CD cord-312431-de7zhswl 187 5 000 000 CD cord-312431-de7zhswl 187 6 - - HYPH cord-312431-de7zhswl 187 7 600 600 CD cord-312431-de7zhswl 187 8 000Â 000â CD cord-312431-de7zhswl 187 9 magnification magnification NN cord-312431-de7zhswl 187 10 . . . cord-312431-de7zhswl 188 1 Scale scale NN cord-312431-de7zhswl 188 2 bar bar NN cord-312431-de7zhswl 188 3 : : : cord-312431-de7zhswl 188 4 50 50 CD cord-312431-de7zhswl 188 5 nm nm NN cord-312431-de7zhswl 188 6 . . . cord-312431-de7zhswl 189 1 Figure figure NN cord-312431-de7zhswl 189 2 10 10 CD cord-312431-de7zhswl 189 3 . . . cord-312431-de7zhswl 190 1 TEM tem NN cord-312431-de7zhswl 190 2 images image NNS cord-312431-de7zhswl 190 3 of of IN cord-312431-de7zhswl 190 4 enveloped envelop VBN cord-312431-de7zhswl 190 5 presumptive presumptive JJ cord-312431-de7zhswl 190 6 ( ( -LRB- cord-312431-de7zhswl 190 7 a a NN cord-312431-de7zhswl 190 8 , , , cord-312431-de7zhswl 190 9 b b NN cord-312431-de7zhswl 190 10 , , , cord-312431-de7zhswl 190 11 c c LS cord-312431-de7zhswl 190 12 ) ) -RRB- cord-312431-de7zhswl 190 13 Poxviridae Poxviridae NNP cord-312431-de7zhswl 190 14 - - HYPH cord-312431-de7zhswl 190 15 like like JJ cord-312431-de7zhswl 190 16 particles particle NNS cord-312431-de7zhswl 190 17 , , , cord-312431-de7zhswl 190 18 ( ( -LRB- cord-312431-de7zhswl 190 19 d d NN cord-312431-de7zhswl 190 20 ) ) -RRB- cord-312431-de7zhswl 190 21 known know VBN cord-312431-de7zhswl 190 22 poxvirus poxvirus NNP cord-312431-de7zhswl 190 23 [ [ -LRB- cord-312431-de7zhswl 190 24 53 53 CD cord-312431-de7zhswl 190 25 ] ] -RRB- cord-312431-de7zhswl 190 26 and and CC cord-312431-de7zhswl 190 27 ( ( -LRB- cord-312431-de7zhswl 190 28 e e NN cord-312431-de7zhswl 190 29 , , , cord-312431-de7zhswl 190 30 f f NNP cord-312431-de7zhswl 190 31 , , , cord-312431-de7zhswl 190 32 g g NNP cord-312431-de7zhswl 190 33 ) ) -RRB- cord-312431-de7zhswl 190 34 Herpesviridae Herpesviridae NNP cord-312431-de7zhswl 190 35 - - HYPH cord-312431-de7zhswl 190 36 like like JJ cord-312431-de7zhswl 190 37 particles particle NNS cord-312431-de7zhswl 190 38 , , , cord-312431-de7zhswl 190 39 ( ( -LRB- cord-312431-de7zhswl 190 40 h h NN cord-312431-de7zhswl 190 41 ) ) -RRB- cord-312431-de7zhswl 190 42 known know VBN cord-312431-de7zhswl 190 43 herpesvirus herpesvirus NN cord-312431-de7zhswl 190 44 [ [ -LRB- cord-312431-de7zhswl 190 45 53 53 CD cord-312431-de7zhswl 190 46 ] ] -RRB- cord-312431-de7zhswl 190 47 , , , cord-312431-de7zhswl 190 48 present present JJ cord-312431-de7zhswl 190 49 in in IN cord-312431-de7zhswl 190 50 the the DT cord-312431-de7zhswl 190 51 Umgeni Umgeni NNP cord-312431-de7zhswl 190 52 River River NNP cord-312431-de7zhswl 190 53 at at IN cord-312431-de7zhswl 190 54 site site NN cord-312431-de7zhswl 190 55 U3 u3 NN cord-312431-de7zhswl 190 56 during during IN cord-312431-de7zhswl 190 57 summer summer NN cord-312431-de7zhswl 190 58 . . . cord-312431-de7zhswl 191 1 Images image NNS cord-312431-de7zhswl 191 2 captured capture VBN cord-312431-de7zhswl 191 3 at at IN cord-312431-de7zhswl 191 4 300 300 CD cord-312431-de7zhswl 191 5 000 000 CD cord-312431-de7zhswl 191 6 - - HYPH cord-312431-de7zhswl 191 7 500 500 CD cord-312431-de7zhswl 191 8 000Â 000â CD cord-312431-de7zhswl 191 9 magnification magnification NN cord-312431-de7zhswl 191 10 . . . cord-312431-de7zhswl 192 1 Scale scale NN cord-312431-de7zhswl 192 2 bar bar NN cord-312431-de7zhswl 192 3 : : : cord-312431-de7zhswl 192 4 200 200 CD cord-312431-de7zhswl 192 5 nm nm NN cord-312431-de7zhswl 192 6 . . . cord-312431-de7zhswl 193 1 Detecting detect VBG cord-312431-de7zhswl 193 2 Virus Virus NNP cord-312431-de7zhswl 193 3 - - HYPH cord-312431-de7zhswl 193 4 Like like JJ cord-312431-de7zhswl 193 5 Particles particle NNS cord-312431-de7zhswl 193 6 401 401 CD cord-312431-de7zhswl 193 7 Figure figure NN cord-312431-de7zhswl 193 8 11 11 CD cord-312431-de7zhswl 193 9 . . . cord-312431-de7zhswl 194 1 TEM tem NN cord-312431-de7zhswl 194 2 images image NNS cord-312431-de7zhswl 194 3 of of IN cord-312431-de7zhswl 194 4 presumptive presumptive JJ cord-312431-de7zhswl 194 5 ( ( -LRB- cord-312431-de7zhswl 194 6 a a NN cord-312431-de7zhswl 194 7 , , , cord-312431-de7zhswl 194 8 b b NN cord-312431-de7zhswl 194 9 , , , cord-312431-de7zhswl 194 10 c c LS cord-312431-de7zhswl 194 11 ) ) -RRB- cord-312431-de7zhswl 194 12 Reoviridae Reoviridae NNP cord-312431-de7zhswl 194 13 VLPs vlp NNS cord-312431-de7zhswl 194 14 , , , cord-312431-de7zhswl 194 15 ( ( -LRB- cord-312431-de7zhswl 194 16 d d NN cord-312431-de7zhswl 194 17 ) ) -RRB- cord-312431-de7zhswl 194 18 known know VBN cord-312431-de7zhswl 194 19 rotavirus rotavirus NN cord-312431-de7zhswl 194 20 [ [ -LRB- cord-312431-de7zhswl 194 21 54 54 CD cord-312431-de7zhswl 194 22 ] ] -RRB- cord-312431-de7zhswl 194 23 , , , cord-312431-de7zhswl 194 24 ( ( -LRB- cord-312431-de7zhswl 194 25 e e NN cord-312431-de7zhswl 194 26 , , , cord-312431-de7zhswl 194 27 f f NNP cord-312431-de7zhswl 194 28 , , , cord-312431-de7zhswl 194 29 g g NNP cord-312431-de7zhswl 194 30 ) ) -RRB- cord-312431-de7zhswl 194 31 presumptive presumptive JJ cord-312431-de7zhswl 194 32 Caliciviridae Caliciviridae NNP cord-312431-de7zhswl 194 33 VLPs vlp NNS cord-312431-de7zhswl 194 34 , , , cord-312431-de7zhswl 194 35 ( ( -LRB- cord-312431-de7zhswl 194 36 h h NN cord-312431-de7zhswl 194 37 ) ) -RRB- cord-312431-de7zhswl 194 38 known know VBN cord-312431-de7zhswl 194 39 norovirus norovirus NN cord-312431-de7zhswl 194 40 [ [ -LRB- cord-312431-de7zhswl 194 41 55 55 CD cord-312431-de7zhswl 194 42 ] ] -RRB- cord-312431-de7zhswl 194 43 , , , cord-312431-de7zhswl 194 44 present present JJ cord-312431-de7zhswl 194 45 in in IN cord-312431-de7zhswl 194 46 the the DT cord-312431-de7zhswl 194 47 Umgeni Umgeni NNP cord-312431-de7zhswl 194 48 River River NNP cord-312431-de7zhswl 194 49 at at IN cord-312431-de7zhswl 194 50 the the DT cord-312431-de7zhswl 194 51 different different JJ cord-312431-de7zhswl 194 52 sampling sampling NN cord-312431-de7zhswl 194 53 sites site NNS cord-312431-de7zhswl 194 54 during during IN cord-312431-de7zhswl 194 55 all all DT cord-312431-de7zhswl 194 56 seasons season NNS cord-312431-de7zhswl 194 57 tested test VBN cord-312431-de7zhswl 194 58 . . . cord-312431-de7zhswl 195 1 Images image NNS cord-312431-de7zhswl 195 2 captured capture VBN cord-312431-de7zhswl 195 3 at at IN cord-312431-de7zhswl 195 4 500 500 CD cord-312431-de7zhswl 195 5 000 000 CD cord-312431-de7zhswl 195 6 - - HYPH cord-312431-de7zhswl 195 7 600 600 CD cord-312431-de7zhswl 195 8 000Â 000â CD cord-312431-de7zhswl 195 9 magnification magnification NN cord-312431-de7zhswl 195 10 . . . cord-312431-de7zhswl 196 1 Scale scale NN cord-312431-de7zhswl 196 2 bar bar NN cord-312431-de7zhswl 196 3 : : : cord-312431-de7zhswl 196 4 50 50 CD cord-312431-de7zhswl 196 5 nm nm NN cord-312431-de7zhswl 196 6 . . . cord-312431-de7zhswl 197 1 Figure figure NN cord-312431-de7zhswl 197 2 12 12 CD cord-312431-de7zhswl 197 3 . . . cord-312431-de7zhswl 198 1 TEM tem NN cord-312431-de7zhswl 198 2 images image NNS cord-312431-de7zhswl 198 3 of of IN cord-312431-de7zhswl 198 4 presumptive presumptive JJ cord-312431-de7zhswl 198 5 enveloped envelop VBN cord-312431-de7zhswl 198 6 VLPs vlp NNS cord-312431-de7zhswl 198 7 ( ( -LRB- cord-312431-de7zhswl 198 8 a a DT cord-312431-de7zhswl 198 9 , , , cord-312431-de7zhswl 198 10 b b NN cord-312431-de7zhswl 198 11 , , , cord-312431-de7zhswl 198 12 c c LS cord-312431-de7zhswl 198 13 ) ) -RRB- cord-312431-de7zhswl 198 14 Coronaviridae Coronaviridae NNP cord-312431-de7zhswl 198 15 VLPs vlp NNS cord-312431-de7zhswl 198 16 , , , cord-312431-de7zhswl 198 17 ( ( -LRB- cord-312431-de7zhswl 198 18 d d NN cord-312431-de7zhswl 198 19 ) ) -RRB- cord-312431-de7zhswl 198 20 known know VBN cord-312431-de7zhswl 198 21 coronavirus coronavirus NN cord-312431-de7zhswl 198 22 [ [ -LRB- cord-312431-de7zhswl 198 23 52 52 CD cord-312431-de7zhswl 198 24 ] ] -RRB- cord-312431-de7zhswl 198 25 , , , cord-312431-de7zhswl 198 26 ( ( -LRB- cord-312431-de7zhswl 198 27 e e NN cord-312431-de7zhswl 198 28 , , , cord-312431-de7zhswl 198 29 f f NNP cord-312431-de7zhswl 198 30 , , , cord-312431-de7zhswl 198 31 g g NNP cord-312431-de7zhswl 198 32 ) ) -RRB- cord-312431-de7zhswl 198 33 presumptive presumptive JJ cord-312431-de7zhswl 198 34 Orthomyxoviridae Orthomyxoviridae NNP cord-312431-de7zhswl 198 35 VLPs vlp NNS cord-312431-de7zhswl 198 36 , , , cord-312431-de7zhswl 198 37 ( ( -LRB- cord-312431-de7zhswl 198 38 h h NN cord-312431-de7zhswl 198 39 ) ) -RRB- cord-312431-de7zhswl 198 40 known know VBN cord-312431-de7zhswl 198 41 influenzavirus influenzavirus NNP cord-312431-de7zhswl 198 42 [ [ -LRB- cord-312431-de7zhswl 198 43 52 52 CD cord-312431-de7zhswl 198 44 ] ] -RRB- cord-312431-de7zhswl 198 45 , , , cord-312431-de7zhswl 198 46 present present JJ cord-312431-de7zhswl 198 47 in in IN cord-312431-de7zhswl 198 48 the the DT cord-312431-de7zhswl 198 49 Umgeni Umgeni NNP cord-312431-de7zhswl 198 50 River River NNP cord-312431-de7zhswl 198 51 at at IN cord-312431-de7zhswl 198 52 the the DT cord-312431-de7zhswl 198 53 different different JJ cord-312431-de7zhswl 198 54 sampling sampling NN cord-312431-de7zhswl 198 55 sites site NNS cord-312431-de7zhswl 198 56 during during IN cord-312431-de7zhswl 198 57 all all DT cord-312431-de7zhswl 198 58 seasons season NNS cord-312431-de7zhswl 198 59 tested test VBN cord-312431-de7zhswl 198 60 . . . cord-312431-de7zhswl 199 1 Images image NNS cord-312431-de7zhswl 199 2 captured capture VBN cord-312431-de7zhswl 199 3 at at IN cord-312431-de7zhswl 199 4 400 400 CD cord-312431-de7zhswl 199 5 000 000 CD cord-312431-de7zhswl 199 6 - - HYPH cord-312431-de7zhswl 199 7 600 600 CD cord-312431-de7zhswl 199 8 000Â 000â CD cord-312431-de7zhswl 199 9 magnification magnification NN cord-312431-de7zhswl 199 10 . . . cord-312431-de7zhswl 200 1 ( ( -LRB- cord-312431-de7zhswl 200 2 Fig Fig NNP cord-312431-de7zhswl 200 3 . . NNP cord-312431-de7zhswl 200 4 13 13 CD cord-312431-de7zhswl 200 5 ) ) -RRB- cord-312431-de7zhswl 200 6 . . . cord-312431-de7zhswl 201 1 Microbiological microbiological JJ cord-312431-de7zhswl 201 2 contamination contamination NN cord-312431-de7zhswl 201 3 of of IN cord-312431-de7zhswl 201 4 water water NN cord-312431-de7zhswl 201 5 has have VBZ cord-312431-de7zhswl 201 6 long long RB cord-312431-de7zhswl 201 7 been be VBN cord-312431-de7zhswl 201 8 a a DT cord-312431-de7zhswl 201 9 concern concern NN cord-312431-de7zhswl 201 10 to to IN cord-312431-de7zhswl 201 11 the the DT cord-312431-de7zhswl 201 12 public public NN cord-312431-de7zhswl 201 13 and and CC cord-312431-de7zhswl 201 14 contamination contamination NN cord-312431-de7zhswl 201 15 of of IN cord-312431-de7zhswl 201 16 water water NN cord-312431-de7zhswl 201 17 resources resource NNS cord-312431-de7zhswl 201 18 , , , cord-312431-de7zhswl 201 19 intended intend VBN cord-312431-de7zhswl 201 20 for for IN cord-312431-de7zhswl 201 21 use use NN cord-312431-de7zhswl 201 22 by by IN cord-312431-de7zhswl 201 23 general general JJ cord-312431-de7zhswl 201 24 population population NN cord-312431-de7zhswl 201 25 , , , cord-312431-de7zhswl 201 26 with with IN cord-312431-de7zhswl 201 27 enteric enteric JJ cord-312431-de7zhswl 201 28 viruses virus NNS cord-312431-de7zhswl 201 29 is be VBZ cord-312431-de7zhswl 201 30 a a DT cord-312431-de7zhswl 201 31 great great JJ cord-312431-de7zhswl 201 32 public public JJ cord-312431-de7zhswl 201 33 health health NN cord-312431-de7zhswl 201 34 problem problem NN cord-312431-de7zhswl 201 35 . . . cord-312431-de7zhswl 202 1 Although although IN cord-312431-de7zhswl 202 2 the the DT cord-312431-de7zhswl 202 3 occurrence occurrence NN cord-312431-de7zhswl 202 4 of of IN cord-312431-de7zhswl 202 5 human human JJ cord-312431-de7zhswl 202 6 enteric enteric JJ cord-312431-de7zhswl 202 7 viruses virus NNS cord-312431-de7zhswl 202 8 has have VBZ cord-312431-de7zhswl 202 9 been be VBN cord-312431-de7zhswl 202 10 demonstrated demonstrate VBN cord-312431-de7zhswl 202 11 in in IN cord-312431-de7zhswl 202 12 surface surface NN cord-312431-de7zhswl 202 13 water water NN cord-312431-de7zhswl 202 14 bodies body NNS cord-312431-de7zhswl 202 15 as as RB cord-312431-de7zhswl 202 16 well well RB cord-312431-de7zhswl 202 17 as as IN cord-312431-de7zhswl 202 18 in in IN cord-312431-de7zhswl 202 19 drinking drink VBG cord-312431-de7zhswl 202 20 water water NN cord-312431-de7zhswl 202 21 supplies supply NNS cord-312431-de7zhswl 202 22 throughout throughout IN cord-312431-de7zhswl 202 23 the the DT cord-312431-de7zhswl 202 24 world world NN cord-312431-de7zhswl 202 25 and and CC cord-312431-de7zhswl 202 26 several several JJ cord-312431-de7zhswl 202 27 outbreaks outbreak NNS cord-312431-de7zhswl 202 28 of of IN cord-312431-de7zhswl 202 29 enteric enteric JJ cord-312431-de7zhswl 202 30 viral viral JJ cord-312431-de7zhswl 202 31 diseases disease NNS cord-312431-de7zhswl 202 32 ( ( -LRB- cord-312431-de7zhswl 202 33 attributed attribute VBN cord-312431-de7zhswl 202 34 to to IN cord-312431-de7zhswl 202 35 either either DT cord-312431-de7zhswl 202 36 contamination contamination NN cord-312431-de7zhswl 202 37 of of IN cord-312431-de7zhswl 202 38 drinking drink VBG cord-312431-de7zhswl 202 39 water water NN cord-312431-de7zhswl 202 40 or or CC cord-312431-de7zhswl 202 41 vegetables vegetable NNS cord-312431-de7zhswl 202 42 and and CC cord-312431-de7zhswl 202 43 seafood seafood NN cord-312431-de7zhswl 202 44 grown grow VBN cord-312431-de7zhswl 202 45 in in IN cord-312431-de7zhswl 202 46 such such JJ cord-312431-de7zhswl 202 47 waters water NNS cord-312431-de7zhswl 202 48 ) ) -RRB- cord-312431-de7zhswl 202 49 have have VBP cord-312431-de7zhswl 202 50 been be VBN cord-312431-de7zhswl 202 51 recorded record VBN cord-312431-de7zhswl 202 52 [ [ -LRB- cord-312431-de7zhswl 202 53 56 56 CD cord-312431-de7zhswl 202 54 , , , cord-312431-de7zhswl 202 55 57 57 CD cord-312431-de7zhswl 202 56 ] ] -RRB- cord-312431-de7zhswl 202 57 . . . cord-312431-de7zhswl 203 1 Routine routine JJ cord-312431-de7zhswl 203 2 examination examination NN cord-312431-de7zhswl 203 3 of of IN cord-312431-de7zhswl 203 4 water water NN cord-312431-de7zhswl 203 5 samples sample NNS cord-312431-de7zhswl 203 6 for for IN cord-312431-de7zhswl 203 7 the the DT cord-312431-de7zhswl 203 8 presence presence NN cord-312431-de7zhswl 203 9 of of IN cord-312431-de7zhswl 203 10 enteric enteric JJ cord-312431-de7zhswl 203 11 viruses virus NNS cord-312431-de7zhswl 203 12 is be VBZ cord-312431-de7zhswl 203 13 not not RB cord-312431-de7zhswl 203 14 largely largely RB cord-312431-de7zhswl 203 15 performed perform VBN cord-312431-de7zhswl 203 16 in in IN cord-312431-de7zhswl 203 17 both both DT cord-312431-de7zhswl 203 18 developed developed JJ cord-312431-de7zhswl 203 19 and and CC cord-312431-de7zhswl 203 20 developing develop VBG cord-312431-de7zhswl 203 21 countries country NNS cord-312431-de7zhswl 203 22 [ [ -LRB- cord-312431-de7zhswl 203 23 57 57 CD cord-312431-de7zhswl 203 24 ] ] -RRB- cord-312431-de7zhswl 203 25 . . . cord-312431-de7zhswl 204 1 In in IN cord-312431-de7zhswl 204 2 this this DT cord-312431-de7zhswl 204 3 study study NN cord-312431-de7zhswl 204 4 , , , cord-312431-de7zhswl 204 5 the the DT cord-312431-de7zhswl 204 6 populations population NNS cord-312431-de7zhswl 204 7 of of IN cord-312431-de7zhswl 204 8 somatic somatic JJ cord-312431-de7zhswl 204 9 and and CC cord-312431-de7zhswl 204 10 F F NNP cord-312431-de7zhswl 204 11 - - HYPH cord-312431-de7zhswl 204 12 RNA RNA NNP cord-312431-de7zhswl 204 13 coliphages coliphage NNS cord-312431-de7zhswl 204 14 as as RB cord-312431-de7zhswl 204 15 well well RB cord-312431-de7zhswl 204 16 as as IN cord-312431-de7zhswl 204 17 various various JJ cord-312431-de7zhswl 204 18 enteric enteric JJ cord-312431-de7zhswl 204 19 viruses virus NNS cord-312431-de7zhswl 204 20 were be VBD cord-312431-de7zhswl 204 21 monitored monitor VBN cord-312431-de7zhswl 204 22 using use VBG cord-312431-de7zhswl 204 23 traditional traditional JJ cord-312431-de7zhswl 204 24 techniques technique NNS cord-312431-de7zhswl 204 25 such such JJ cord-312431-de7zhswl 204 26 as as IN cord-312431-de7zhswl 204 27 electron electron NN cord-312431-de7zhswl 204 28 microscopy microscopy NN cord-312431-de7zhswl 204 29 and and CC cord-312431-de7zhswl 204 30 tissue tissue NN cord-312431-de7zhswl 204 31 culture culture NN cord-312431-de7zhswl 204 32 . . . cord-312431-de7zhswl 205 1 Phages phage NNS cord-312431-de7zhswl 205 2 have have VBP cord-312431-de7zhswl 205 3 been be VBN cord-312431-de7zhswl 205 4 proposed propose VBN cord-312431-de7zhswl 205 5 as as IN cord-312431-de7zhswl 205 6 microbial microbial JJ cord-312431-de7zhswl 205 7 indicators indicator NNS cord-312431-de7zhswl 205 8 of of IN cord-312431-de7zhswl 205 9 water water NN cord-312431-de7zhswl 205 10 quality quality NN cord-312431-de7zhswl 205 11 , , , cord-312431-de7zhswl 205 12 as as IN cord-312431-de7zhswl 205 13 they -PRON- PRP cord-312431-de7zhswl 205 14 share share VBP cord-312431-de7zhswl 205 15 many many JJ cord-312431-de7zhswl 205 16 fundamental fundamental JJ cord-312431-de7zhswl 205 17 properties property NNS cord-312431-de7zhswl 205 18 with with IN cord-312431-de7zhswl 205 19 human human JJ cord-312431-de7zhswl 205 20 enteric enteric JJ cord-312431-de7zhswl 205 21 viruses virus NNS cord-312431-de7zhswl 205 22 which which WDT cord-312431-de7zhswl 205 23 pose pose VBP cord-312431-de7zhswl 205 24 a a DT cord-312431-de7zhswl 205 25 health health NN cord-312431-de7zhswl 205 26 risk risk NN cord-312431-de7zhswl 205 27 , , , cord-312431-de7zhswl 205 28 if if IN cord-312431-de7zhswl 205 29 present present JJ cord-312431-de7zhswl 205 30 in in IN cord-312431-de7zhswl 205 31 water water NN cord-312431-de7zhswl 205 32 contaminated contaminate VBN cord-312431-de7zhswl 205 33 with with IN cord-312431-de7zhswl 205 34 human human JJ cord-312431-de7zhswl 205 35 faeces faece NNS cord-312431-de7zhswl 205 36 [ [ -LRB- cord-312431-de7zhswl 205 37 18 18 CD cord-312431-de7zhswl 205 38 ] ] -RRB- cord-312431-de7zhswl 205 39 . . . cord-312431-de7zhswl 206 1 All all DT cord-312431-de7zhswl 206 2 sampling sampling NN cord-312431-de7zhswl 206 3 points point NNS cord-312431-de7zhswl 206 4 throughout throughout IN cord-312431-de7zhswl 206 5 all all DT cord-312431-de7zhswl 206 6 seasons season NNS cord-312431-de7zhswl 206 7 tested test VBD cord-312431-de7zhswl 206 8 positive positive JJ cord-312431-de7zhswl 206 9 for for IN cord-312431-de7zhswl 206 10 the the DT cord-312431-de7zhswl 206 11 presence presence NN cord-312431-de7zhswl 206 12 of of IN cord-312431-de7zhswl 206 13 somatic somatic JJ cord-312431-de7zhswl 206 14 coliphages coliphage NNS cord-312431-de7zhswl 206 15 which which WDT cord-312431-de7zhswl 206 16 ranged range VBD cord-312431-de7zhswl 206 17 from from IN cord-312431-de7zhswl 206 18 10 10 CD cord-312431-de7zhswl 206 19 pfu pfu NN cord-312431-de7zhswl 206 20 / / SYM cord-312431-de7zhswl 206 21 mL ml NN cord-312431-de7zhswl 206 22 ( ( -LRB- cord-312431-de7zhswl 206 23 U5-autumn U5-autumn NNP cord-312431-de7zhswl 206 24 ) ) -RRB- cord-312431-de7zhswl 206 25 to to IN cord-312431-de7zhswl 206 26 659 659 CD cord-312431-de7zhswl 206 27 pfu pfu NN cord-312431-de7zhswl 206 28 / / SYM cord-312431-de7zhswl 206 29 mL ml NN cord-312431-de7zhswl 206 30 ( ( -LRB- cord-312431-de7zhswl 206 31 U1-summer u1-summer JJ cord-312431-de7zhswl 206 32 ) ) -RRB- cord-312431-de7zhswl 206 33 especially especially RB cord-312431-de7zhswl 206 34 in in IN cord-312431-de7zhswl 206 35 the the DT cord-312431-de7zhswl 206 36 lower low JJR cord-312431-de7zhswl 206 37 reaches reach NNS cord-312431-de7zhswl 206 38 of of IN cord-312431-de7zhswl 206 39 the the DT cord-312431-de7zhswl 206 40 river river NN cord-312431-de7zhswl 206 41 and and CC cord-312431-de7zhswl 206 42 during during IN cord-312431-de7zhswl 206 43 summer summer NN cord-312431-de7zhswl 206 44 . . . cord-312431-de7zhswl 207 1 F F NNP cord-312431-de7zhswl 207 2 - - HYPH cord-312431-de7zhswl 207 3 RNA RNA NNP cord-312431-de7zhswl 207 4 coliphage coliphage NN cord-312431-de7zhswl 207 5 counts count NNS cord-312431-de7zhswl 207 6 were be VBD cord-312431-de7zhswl 207 7 significantly significantly RB cord-312431-de7zhswl 207 8 lower low JJR cord-312431-de7zhswl 207 9 compared compare VBN cord-312431-de7zhswl 207 10 to to IN cord-312431-de7zhswl 207 11 those those DT cord-312431-de7zhswl 207 12 of of IN cord-312431-de7zhswl 207 13 somatic somatic JJ cord-312431-de7zhswl 207 14 coliphage coliphage NN cord-312431-de7zhswl 207 15 counts count NNS cord-312431-de7zhswl 207 16 . . . cord-312431-de7zhswl 208 1 F F NNP cord-312431-de7zhswl 208 2 - - HYPH cord-312431-de7zhswl 208 3 RNA RNA NNP cord-312431-de7zhswl 208 4 coliphages coliphage NNS cord-312431-de7zhswl 208 5 , , , cord-312431-de7zhswl 208 6 from from IN cord-312431-de7zhswl 208 7 0 0 CD cord-312431-de7zhswl 208 8 pfu pfu NN cord-312431-de7zhswl 208 9 / / SYM cord-312431-de7zhswl 208 10 mL ml NN cord-312431-de7zhswl 208 11 ( ( -LRB- cord-312431-de7zhswl 208 12 U5-autumn U5-autumn NNP cord-312431-de7zhswl 208 13 ) ) -RRB- cord-312431-de7zhswl 208 14 to to IN cord-312431-de7zhswl 208 15 550 550 CD cord-312431-de7zhswl 208 16 pfu pfu NN cord-312431-de7zhswl 208 17 / / SYM cord-312431-de7zhswl 208 18 mL ml NN cord-312431-de7zhswl 208 19 ( ( -LRB- cord-312431-de7zhswl 208 20 U2-summer U2-summer NNP cord-312431-de7zhswl 208 21 ) ) -RRB- cord-312431-de7zhswl 208 22 , , , cord-312431-de7zhswl 208 23 were be VBD cord-312431-de7zhswl 208 24 also also RB cord-312431-de7zhswl 208 25 detected detect VBN cord-312431-de7zhswl 208 26 more more RBR cord-312431-de7zhswl 208 27 frequently frequently RB cord-312431-de7zhswl 208 28 in in IN cord-312431-de7zhswl 208 29 the the DT cord-312431-de7zhswl 208 30 lower low JJR cord-312431-de7zhswl 208 31 reaches reach NNS cord-312431-de7zhswl 208 32 of of IN cord-312431-de7zhswl 208 33 the the DT cord-312431-de7zhswl 208 34 river river NN cord-312431-de7zhswl 208 35 . . . cord-312431-de7zhswl 209 1 Mean mean JJ cord-312431-de7zhswl 209 2 concentrations concentration NNS cord-312431-de7zhswl 209 3 of of IN cord-312431-de7zhswl 209 4 somatic somatic JJ cord-312431-de7zhswl 209 5 coliphages coliphage NNS cord-312431-de7zhswl 209 6 and and CC cord-312431-de7zhswl 209 7 F F NNP cord-312431-de7zhswl 209 8 - - HYPH cord-312431-de7zhswl 209 9 RNA RNA NNP cord-312431-de7zhswl 209 10 coliphages coliphage NNS cord-312431-de7zhswl 209 11 were be VBD cord-312431-de7zhswl 209 12 comparable comparable JJ cord-312431-de7zhswl 209 13 between between IN cord-312431-de7zhswl 209 14 autumn autumn NN cord-312431-de7zhswl 209 15 and and CC cord-312431-de7zhswl 209 16 spring spring NN cord-312431-de7zhswl 209 17 and and CC cord-312431-de7zhswl 209 18 correlation correlation NN cord-312431-de7zhswl 209 19 analysis analysis NN cord-312431-de7zhswl 209 20 resulted result VBD cord-312431-de7zhswl 209 21 in in IN cord-312431-de7zhswl 209 22 a a DT cord-312431-de7zhswl 209 23 strong strong JJ cord-312431-de7zhswl 209 24 Pearson Pearson NNP cord-312431-de7zhswl 209 25 's 's POS cord-312431-de7zhswl 209 26 correlation correlation NN cord-312431-de7zhswl 209 27 coefficient coefficient NN cord-312431-de7zhswl 209 28 of of IN cord-312431-de7zhswl 209 29 0.977 0.977 CD cord-312431-de7zhswl 210 1 ( ( -LRB- cord-312431-de7zhswl 210 2 p p NN cord-312431-de7zhswl 210 3 < < XX cord-312431-de7zhswl 210 4 0.01 0.01 CD cord-312431-de7zhswl 210 5 ) ) -RRB- cord-312431-de7zhswl 210 6 . . . cord-312431-de7zhswl 211 1 Bacteriophages bacteriophage NNS cord-312431-de7zhswl 211 2 are be VBP cord-312431-de7zhswl 211 3 not not RB cord-312431-de7zhswl 211 4 a a DT cord-312431-de7zhswl 211 5 stable stable JJ cord-312431-de7zhswl 211 6 part part NN cord-312431-de7zhswl 211 7 of of IN cord-312431-de7zhswl 211 8 water water NN cord-312431-de7zhswl 211 9 micro micro NNS cord-312431-de7zhswl 211 10 - - HYPH cord-312431-de7zhswl 211 11 flora flora NNS cord-312431-de7zhswl 211 12 and and CC cord-312431-de7zhswl 211 13 their -PRON- PRP$ cord-312431-de7zhswl 211 14 presence presence NN cord-312431-de7zhswl 211 15 is be VBZ cord-312431-de7zhswl 211 16 usually usually RB cord-312431-de7zhswl 211 17 associated associate VBN cord-312431-de7zhswl 211 18 with with IN cord-312431-de7zhswl 211 19 wastewater wastewater NN cord-312431-de7zhswl 211 20 inflow inflow NN cord-312431-de7zhswl 211 21 rich rich JJ cord-312431-de7zhswl 211 22 in in IN cord-312431-de7zhswl 211 23 animal animal NN cord-312431-de7zhswl 211 24 and and CC cord-312431-de7zhswl 211 25 human human JJ cord-312431-de7zhswl 211 26 excrement excrement NN cord-312431-de7zhswl 211 27 [ [ -LRB- cord-312431-de7zhswl 211 28 58 58 CD cord-312431-de7zhswl 211 29 ] ] -RRB- cord-312431-de7zhswl 211 30 . . . cord-312431-de7zhswl 212 1 Their -PRON- PRP$ cord-312431-de7zhswl 212 2 survivability survivability NN cord-312431-de7zhswl 212 3 depends depend VBZ cord-312431-de7zhswl 212 4 upon upon IN cord-312431-de7zhswl 212 5 physico physico NN cord-312431-de7zhswl 212 6 - - HYPH cord-312431-de7zhswl 212 7 chemical chemical NN cord-312431-de7zhswl 212 8 environmental environmental JJ cord-312431-de7zhswl 212 9 conditions condition NNS cord-312431-de7zhswl 212 10 [ [ -LRB- cord-312431-de7zhswl 212 11 59 59 CD cord-312431-de7zhswl 212 12 ] ] -RRB- cord-312431-de7zhswl 212 13 . . . cord-312431-de7zhswl 213 1 Somatic somatic JJ cord-312431-de7zhswl 213 2 coliphages coliphage NNS cord-312431-de7zhswl 213 3 have have VBP cord-312431-de7zhswl 213 4 been be VBN cord-312431-de7zhswl 213 5 found find VBN cord-312431-de7zhswl 213 6 to to IN cord-312431-de7zhswl 213 7 outnumber outnumber VB cord-312431-de7zhswl 213 8 F F NNP cord-312431-de7zhswl 213 9 - - HYPH cord-312431-de7zhswl 213 10 RNA RNA NNP cord-312431-de7zhswl 213 11 phages phage NNS cord-312431-de7zhswl 213 12 in in IN cord-312431-de7zhswl 213 13 waste waste NN cord-312431-de7zhswl 213 14 water water NN cord-312431-de7zhswl 213 15 and and CC cord-312431-de7zhswl 213 16 raw raw JJ cord-312431-de7zhswl 213 17 water water NN cord-312431-de7zhswl 213 18 sources source NNS cord-312431-de7zhswl 213 19 by by IN cord-312431-de7zhswl 213 20 a a DT cord-312431-de7zhswl 213 21 factor factor NN cord-312431-de7zhswl 213 22 of of IN cord-312431-de7zhswl 213 23 about about IN cord-312431-de7zhswl 213 24 5 5 CD cord-312431-de7zhswl 213 25 , , , cord-312431-de7zhswl 213 26 and and CC cord-312431-de7zhswl 213 27 cytopathogenic cytopathogenic JJ cord-312431-de7zhswl 213 28 human human JJ cord-312431-de7zhswl 213 29 viruses virus NNS cord-312431-de7zhswl 213 30 by by IN cord-312431-de7zhswl 213 31 about about RB cord-312431-de7zhswl 213 32 500 500 CD cord-312431-de7zhswl 213 33 [ [ -LRB- cord-312431-de7zhswl 213 34 60 60 CD cord-312431-de7zhswl 213 35 , , , cord-312431-de7zhswl 213 36 61 61 CD cord-312431-de7zhswl 213 37 ] ] -RRB- cord-312431-de7zhswl 213 38 , , , cord-312431-de7zhswl 213 39 thus thus RB cord-312431-de7zhswl 213 40 making make VBG cord-312431-de7zhswl 213 41 them -PRON- PRP cord-312431-de7zhswl 213 42 valuable valuable JJ cord-312431-de7zhswl 213 43 indicators indicator NNS cord-312431-de7zhswl 213 44 for for IN cord-312431-de7zhswl 213 45 assessing assess VBG cord-312431-de7zhswl 213 46 the the DT cord-312431-de7zhswl 213 47 behaviour behaviour NN cord-312431-de7zhswl 213 48 of of IN cord-312431-de7zhswl 213 49 and and CC cord-312431-de7zhswl 213 50 the the DT cord-312431-de7zhswl 213 51 possible possible JJ cord-312431-de7zhswl 213 52 presence presence NN cord-312431-de7zhswl 213 53 of of IN cord-312431-de7zhswl 213 54 enteric enteric JJ cord-312431-de7zhswl 213 55 viruses virus NNS cord-312431-de7zhswl 213 56 in in IN cord-312431-de7zhswl 213 57 water water NN cord-312431-de7zhswl 213 58 environments environment NNS cord-312431-de7zhswl 213 59 . . . cord-312431-de7zhswl 214 1 Male male NN cord-312431-de7zhswl 214 2 - - HYPH cord-312431-de7zhswl 214 3 specific specific JJ cord-312431-de7zhswl 214 4 ( ( -LRB- cord-312431-de7zhswl 214 5 F F NNP cord-312431-de7zhswl 214 6 - - HYPH cord-312431-de7zhswl 214 7 RNA RNA NNP cord-312431-de7zhswl 214 8 ) ) -RRB- cord-312431-de7zhswl 214 9 coliphages coliphage NNS cord-312431-de7zhswl 214 10 are be VBP cord-312431-de7zhswl 214 11 highly highly RB cord-312431-de7zhswl 214 12 specific specific JJ cord-312431-de7zhswl 214 13 for for IN cord-312431-de7zhswl 214 14 sewage sewage NN cord-312431-de7zhswl 214 15 pollution pollution NN cord-312431-de7zhswl 214 16 and and CC cord-312431-de7zhswl 214 17 can can MD cord-312431-de7zhswl 214 18 not not RB cord-312431-de7zhswl 214 19 be be VB cord-312431-de7zhswl 214 20 replicated replicate VBN cord-312431-de7zhswl 214 21 in in IN cord-312431-de7zhswl 214 22 water water NN cord-312431-de7zhswl 214 23 environments environment NNS cord-312431-de7zhswl 214 24 , , , cord-312431-de7zhswl 214 25 but but CC cord-312431-de7zhswl 214 26 detection detection NN cord-312431-de7zhswl 214 27 methods method NNS cord-312431-de7zhswl 214 28 are be VBP cord-312431-de7zhswl 214 29 more more RBR cord-312431-de7zhswl 214 30 complicated complicated JJ cord-312431-de7zhswl 214 31 [ [ -LRB- cord-312431-de7zhswl 214 32 62 62 CD cord-312431-de7zhswl 214 33 ] ] -RRB- cord-312431-de7zhswl 214 34 . . . cord-312431-de7zhswl 215 1 VLPs vlp NNS cord-312431-de7zhswl 215 2 were be VBD cord-312431-de7zhswl 215 3 detected detect VBN cord-312431-de7zhswl 215 4 using use VBG cord-312431-de7zhswl 215 5 EM EM NNP cord-312431-de7zhswl 215 6 at at IN cord-312431-de7zhswl 215 7 all all DT cord-312431-de7zhswl 215 8 sampling sampling NN cord-312431-de7zhswl 215 9 sites site NNS cord-312431-de7zhswl 215 10 throughout throughout IN cord-312431-de7zhswl 215 11 all all DT cord-312431-de7zhswl 215 12 seasons season NNS cord-312431-de7zhswl 215 13 , , , cord-312431-de7zhswl 215 14 with with IN cord-312431-de7zhswl 215 15 point point NN cord-312431-de7zhswl 215 16 U1 u1 NN cord-312431-de7zhswl 215 17 during during IN cord-312431-de7zhswl 215 18 summer summer NN cord-312431-de7zhswl 215 19 having have VBG cord-312431-de7zhswl 215 20 the the DT cord-312431-de7zhswl 215 21 highest high JJS cord-312431-de7zhswl 215 22 population population NN cord-312431-de7zhswl 215 23 of of IN cord-312431-de7zhswl 215 24 2086 2086 CD cord-312431-de7zhswl 215 25 VLP VLP NNP cord-312431-de7zhswl 215 26 / / SYM cord-312431-de7zhswl 215 27 mL. mL. NNP cord-312431-de7zhswl 215 28 Umgeni Umgeni NNP cord-312431-de7zhswl 215 29 River River NNP cord-312431-de7zhswl 215 30 points point VBZ cord-312431-de7zhswl 215 31 U1 u1 NN cord-312431-de7zhswl 215 32 , , , cord-312431-de7zhswl 215 33 U2 U2 NNP cord-312431-de7zhswl 215 34 and and CC cord-312431-de7zhswl 215 35 U3 U3 NNP cord-312431-de7zhswl 215 36 were be VBD cord-312431-de7zhswl 215 37 more more RBR cord-312431-de7zhswl 215 38 contaminated contaminate VBN cord-312431-de7zhswl 215 39 with with IN cord-312431-de7zhswl 215 40 phage phage NN cord-312431-de7zhswl 215 41 and and CC cord-312431-de7zhswl 215 42 virus virus NN cord-312431-de7zhswl 215 43 - - HYPH cord-312431-de7zhswl 215 44 like like JJ cord-312431-de7zhswl 215 45 populations population NNS cord-312431-de7zhswl 215 46 than than IN cord-312431-de7zhswl 215 47 points point NNS cord-312431-de7zhswl 215 48 U4 U4 NNP cord-312431-de7zhswl 215 49 and and CC cord-312431-de7zhswl 215 50 U5 U5 NNS cord-312431-de7zhswl 215 51 . . . cord-312431-de7zhswl 216 1 Increasing increase VBG cord-312431-de7zhswl 216 2 in in IN cord-312431-de7zhswl 216 3 the the DT cord-312431-de7zhswl 216 4 phage phage NN cord-312431-de7zhswl 216 5 and and CC cord-312431-de7zhswl 216 6 VLP VLP NNP cord-312431-de7zhswl 216 7 populations population NNS cord-312431-de7zhswl 216 8 from from IN cord-312431-de7zhswl 216 9 upstream upstream RB cord-312431-de7zhswl 216 10 to to IN cord-312431-de7zhswl 216 11 the the DT cord-312431-de7zhswl 216 12 river river NN cord-312431-de7zhswl 216 13 mouth mouth NN cord-312431-de7zhswl 216 14 were be VBD cord-312431-de7zhswl 216 15 observed observe VBN cord-312431-de7zhswl 216 16 in in IN cord-312431-de7zhswl 216 17 all all DT cord-312431-de7zhswl 216 18 seasons season NNS cord-312431-de7zhswl 216 19 . . . cord-312431-de7zhswl 217 1 This this DT cord-312431-de7zhswl 217 2 was be VBD cord-312431-de7zhswl 217 3 probably probably RB cord-312431-de7zhswl 217 4 because because IN cord-312431-de7zhswl 217 5 the the DT cord-312431-de7zhswl 217 6 river river NN cord-312431-de7zhswl 217 7 flows flow VBZ cord-312431-de7zhswl 217 8 through through IN cord-312431-de7zhswl 217 9 the the DT cord-312431-de7zhswl 217 10 more more RBR cord-312431-de7zhswl 217 11 urbanised urbanised JJ cord-312431-de7zhswl 217 12 areas area NNS cord-312431-de7zhswl 217 13 of of IN cord-312431-de7zhswl 217 14 Durban Durban NNP cord-312431-de7zhswl 217 15 and and CC cord-312431-de7zhswl 217 16 is be VBZ cord-312431-de7zhswl 217 17 subject subject JJ cord-312431-de7zhswl 217 18 to to IN cord-312431-de7zhswl 217 19 higher high JJR cord-312431-de7zhswl 217 20 surface surface NN cord-312431-de7zhswl 217 21 runoff runoff NN cord-312431-de7zhswl 217 22 [ [ -LRB- cord-312431-de7zhswl 217 23 63 63 CD cord-312431-de7zhswl 217 24 ] ] -RRB- cord-312431-de7zhswl 217 25 . . . cord-312431-de7zhswl 218 1 It -PRON- PRP cord-312431-de7zhswl 218 2 can can MD cord-312431-de7zhswl 218 3 be be VB cord-312431-de7zhswl 218 4 speculated speculate VBN cord-312431-de7zhswl 218 5 that that IN cord-312431-de7zhswl 218 6 increased increase VBN cord-312431-de7zhswl 218 7 human human JJ cord-312431-de7zhswl 218 8 activity activity NN cord-312431-de7zhswl 218 9 as as RB cord-312431-de7zhswl 218 10 well well RB cord-312431-de7zhswl 218 11 as as IN cord-312431-de7zhswl 218 12 informal informal JJ cord-312431-de7zhswl 218 13 settlements settlement NNS cord-312431-de7zhswl 218 14 and and CC cord-312431-de7zhswl 218 15 trading trading NN cord-312431-de7zhswl 218 16 activities activity NNS cord-312431-de7zhswl 218 17 in in IN cord-312431-de7zhswl 218 18 the the DT cord-312431-de7zhswl 218 19 storm storm NN cord-312431-de7zhswl 218 20 water water NN cord-312431-de7zhswl 218 21 drain drain NN cord-312431-de7zhswl 218 22 catchment catchment NN cord-312431-de7zhswl 218 23 areas area NNS cord-312431-de7zhswl 218 24 contributed contribute VBD cord-312431-de7zhswl 218 25 to to IN cord-312431-de7zhswl 218 26 the the DT cord-312431-de7zhswl 218 27 high high JJ cord-312431-de7zhswl 218 28 levels level NNS cord-312431-de7zhswl 218 29 of of IN cord-312431-de7zhswl 218 30 phage phage NN cord-312431-de7zhswl 218 31 populations population NNS cord-312431-de7zhswl 218 32 downstream downstream VBP cord-312431-de7zhswl 218 33 the the DT cord-312431-de7zhswl 218 34 river river NN cord-312431-de7zhswl 218 35 at at IN cord-312431-de7zhswl 218 36 points point NNS cord-312431-de7zhswl 218 37 U1 U1 NNP cord-312431-de7zhswl 218 38 , , , cord-312431-de7zhswl 218 39 U2 U2 NNP cord-312431-de7zhswl 218 40 and and CC cord-312431-de7zhswl 218 41 U3 U3 NNP cord-312431-de7zhswl 218 42 . . . cord-312431-de7zhswl 219 1 The the DT cord-312431-de7zhswl 219 2 thicket thicket NN cord-312431-de7zhswl 219 3 and and CC cord-312431-de7zhswl 219 4 bushland bushland NN cord-312431-de7zhswl 219 5 that that WDT cord-312431-de7zhswl 219 6 enclosed enclose VBD cord-312431-de7zhswl 219 7 both both CC cord-312431-de7zhswl 219 8 the the DT cord-312431-de7zhswl 219 9 Krantzkloof Krantzkloof NNP cord-312431-de7zhswl 219 10 Nature Nature NNP cord-312431-de7zhswl 219 11 Reserve Reserve NNP cord-312431-de7zhswl 219 12 ( ( -LRB- cord-312431-de7zhswl 219 13 U4 U4 NNP cord-312431-de7zhswl 219 14 ) ) -RRB- cord-312431-de7zhswl 219 15 and and CC cord-312431-de7zhswl 219 16 Inanda Inanda NNP cord-312431-de7zhswl 219 17 Dam Dam NNP cord-312431-de7zhswl 219 18 ( ( -LRB- cord-312431-de7zhswl 219 19 U5 U5 NNP cord-312431-de7zhswl 219 20 ) ) -RRB- cord-312431-de7zhswl 219 21 can can MD cord-312431-de7zhswl 219 22 serve serve VB cord-312431-de7zhswl 219 23 as as IN cord-312431-de7zhswl 219 24 vegetated vegetate VBN cord-312431-de7zhswl 219 25 pervious pervious JJ cord-312431-de7zhswl 219 26 buffers buffer NNS cord-312431-de7zhswl 219 27 as as IN cord-312431-de7zhswl 219 28 passive passive JJ cord-312431-de7zhswl 219 29 treatment treatment NN cord-312431-de7zhswl 219 30 systems system NNS cord-312431-de7zhswl 219 31 allowing allow VBG cord-312431-de7zhswl 219 32 agricultural agricultural JJ cord-312431-de7zhswl 219 33 and and CC cord-312431-de7zhswl 219 34 storm storm NN cord-312431-de7zhswl 219 35 water water NN cord-312431-de7zhswl 219 36 runoff runoff NN cord-312431-de7zhswl 219 37 to to TO cord-312431-de7zhswl 219 38 reach reach VB cord-312431-de7zhswl 219 39 the the DT cord-312431-de7zhswl 219 40 river river NN cord-312431-de7zhswl 219 41 and and CC cord-312431-de7zhswl 219 42 dam dam VB cord-312431-de7zhswl 219 43 in in IN cord-312431-de7zhswl 219 44 a a DT cord-312431-de7zhswl 219 45 less less RBR cord-312431-de7zhswl 219 46 impaired impaired JJ cord-312431-de7zhswl 219 47 state state NN cord-312431-de7zhswl 219 48 [ [ -LRB- cord-312431-de7zhswl 219 49 35 35 CD cord-312431-de7zhswl 219 50 ] ] -RRB- cord-312431-de7zhswl 219 51 . . . cord-312431-de7zhswl 220 1 Mallin Mallin NNP cord-312431-de7zhswl 220 2 et et NNP cord-312431-de7zhswl 220 3 al al NNP cord-312431-de7zhswl 220 4 . . . cord-312431-de7zhswl 221 1 [ [ -LRB- cord-312431-de7zhswl 221 2 64 64 CD cord-312431-de7zhswl 221 3 ] ] -RRB- cord-312431-de7zhswl 221 4 found find VBD cord-312431-de7zhswl 221 5 that that IN cord-312431-de7zhswl 221 6 human human JJ cord-312431-de7zhswl 221 7 development development NN cord-312431-de7zhswl 221 8 along along IN cord-312431-de7zhswl 221 9 the the DT cord-312431-de7zhswl 221 10 land land NN cord-312431-de7zhswl 221 11 - - HYPH cord-312431-de7zhswl 221 12 seawater seawater NN cord-312431-de7zhswl 221 13 interface interface NN cord-312431-de7zhswl 221 14 is be VBZ cord-312431-de7zhswl 221 15 considered consider VBN cord-312431-de7zhswl 221 16 to to TO cord-312431-de7zhswl 221 17 have have VB cord-312431-de7zhswl 221 18 significant significant JJ cord-312431-de7zhswl 221 19 environmental environmental JJ cord-312431-de7zhswl 221 20 consequences consequence NNS cord-312431-de7zhswl 221 21 resulting result VBG cord-312431-de7zhswl 221 22 in in IN cord-312431-de7zhswl 221 23 a a DT cord-312431-de7zhswl 221 24 difference difference NN cord-312431-de7zhswl 221 25 in in IN cord-312431-de7zhswl 221 26 abundance abundance NN cord-312431-de7zhswl 221 27 and and CC cord-312431-de7zhswl 221 28 distribution distribution NN cord-312431-de7zhswl 221 29 of of IN cord-312431-de7zhswl 221 30 the the DT cord-312431-de7zhswl 221 31 enteric enteric JJ cord-312431-de7zhswl 221 32 pathogen pathogen NN cord-312431-de7zhswl 221 33 indicator indicator NN cord-312431-de7zhswl 221 34 microbes microbe NNS cord-312431-de7zhswl 221 35 and and CC cord-312431-de7zhswl 221 36 can can MD cord-312431-de7zhswl 221 37 often often RB cord-312431-de7zhswl 221 38 pose pose VB cord-312431-de7zhswl 221 39 an an DT cord-312431-de7zhswl 221 40 increased increase VBN cord-312431-de7zhswl 221 41 human human JJ cord-312431-de7zhswl 221 42 health health NN cord-312431-de7zhswl 221 43 risk risk NN cord-312431-de7zhswl 221 44 . . . cord-312431-de7zhswl 222 1 All all DT cord-312431-de7zhswl 222 2 variables variable NNS cord-312431-de7zhswl 222 3 tested test VBN cord-312431-de7zhswl 222 4 had have VBD cord-312431-de7zhswl 222 5 positive positive JJ cord-312431-de7zhswl 222 6 correlations correlation NNS cord-312431-de7zhswl 222 7 with with IN cord-312431-de7zhswl 222 8 each each DT cord-312431-de7zhswl 222 9 other other JJ cord-312431-de7zhswl 222 10 during during IN cord-312431-de7zhswl 222 11 all all DT cord-312431-de7zhswl 222 12 seasons season NNS cord-312431-de7zhswl 222 13 . . . cord-312431-de7zhswl 223 1 Significant significant JJ cord-312431-de7zhswl 223 2 positive positive JJ cord-312431-de7zhswl 223 3 correlations correlation NNS cord-312431-de7zhswl 223 4 were be VBD cord-312431-de7zhswl 223 5 observed observe VBN cord-312431-de7zhswl 223 6 between between IN cord-312431-de7zhswl 223 7 the the DT cord-312431-de7zhswl 223 8 somatic somatic JJ cord-312431-de7zhswl 223 9 phage phage NN cord-312431-de7zhswl 223 10 and and CC cord-312431-de7zhswl 223 11 F F NNP cord-312431-de7zhswl 223 12 - - HYPH cord-312431-de7zhswl 223 13 RNA RNA NNP cord-312431-de7zhswl 223 14 phage phage NN cord-312431-de7zhswl 223 15 ( ( -LRB- cord-312431-de7zhswl 223 16 r r NNP cord-312431-de7zhswl 223 17 ¼ ¼ NNP cord-312431-de7zhswl 223 18 0.991 0.991 CD cord-312431-de7zhswl 223 19 , , , cord-312431-de7zhswl 223 20 p p NN cord-312431-de7zhswl 223 21 ¼ ¼ CD cord-312431-de7zhswl 223 22 0.001 0.001 CD cord-312431-de7zhswl 223 23 ) ) -RRB- cord-312431-de7zhswl 223 24 and and CC cord-312431-de7zhswl 223 25 somatic somatic JJ cord-312431-de7zhswl 223 26 phage phage NN cord-312431-de7zhswl 223 27 and and CC cord-312431-de7zhswl 223 28 VLP VLP NNP cord-312431-de7zhswl 223 29 ( ( -LRB- cord-312431-de7zhswl 223 30 r r NNP cord-312431-de7zhswl 223 31 ¼ ¼ NNP cord-312431-de7zhswl 223 32 0.884 0.884 CD cord-312431-de7zhswl 223 33 , , , cord-312431-de7zhswl 223 34 p p NN cord-312431-de7zhswl 223 35 ¼ ¼ CD cord-312431-de7zhswl 223 36 0.46 0.46 CD cord-312431-de7zhswl 223 37 ) ) -RRB- cord-312431-de7zhswl 223 38 . . . cord-312431-de7zhswl 224 1 A a DT cord-312431-de7zhswl 224 2 correlation correlation NN cord-312431-de7zhswl 224 3 between between IN cord-312431-de7zhswl 224 4 phage phage NN cord-312431-de7zhswl 224 5 quantity quantity NN cord-312431-de7zhswl 224 6 and and CC cord-312431-de7zhswl 224 7 degree degree NN cord-312431-de7zhswl 224 8 of of IN cord-312431-de7zhswl 224 9 faecal faecal JJ cord-312431-de7zhswl 224 10 pollution pollution NN cord-312431-de7zhswl 224 11 has have VBZ cord-312431-de7zhswl 224 12 been be VBN cord-312431-de7zhswl 224 13 found find VBN cord-312431-de7zhswl 224 14 elsewhere elsewhere RB cord-312431-de7zhswl 224 15 [ [ -LRB- cord-312431-de7zhswl 224 16 65 65 CD cord-312431-de7zhswl 224 17 ] ] -RRB- cord-312431-de7zhswl 224 18 . . . cord-312431-de7zhswl 225 1 Similar similar JJ cord-312431-de7zhswl 225 2 results result NNS cord-312431-de7zhswl 225 3 were be VBD cord-312431-de7zhswl 225 4 also also RB cord-312431-de7zhswl 225 5 obtained obtain VBN cord-312431-de7zhswl 225 6 by by IN cord-312431-de7zhswl 225 7 Armon Armon NNP cord-312431-de7zhswl 225 8 and and CC cord-312431-de7zhswl 225 9 Knott Knott NNP cord-312431-de7zhswl 225 10 [ [ -LRB- cord-312431-de7zhswl 225 11 60 60 CD cord-312431-de7zhswl 225 12 ] ] -RRB- cord-312431-de7zhswl 225 13 who who WP cord-312431-de7zhswl 225 14 found find VBD cord-312431-de7zhswl 225 15 positive positive JJ cord-312431-de7zhswl 225 16 correlations correlation NNS cord-312431-de7zhswl 225 17 of of IN cord-312431-de7zhswl 225 18 phage phage NN cord-312431-de7zhswl 225 19 number number NN cord-312431-de7zhswl 225 20 and and CC cord-312431-de7zhswl 225 21 bacterial bacterial JJ cord-312431-de7zhswl 225 22 pollution pollution NN cord-312431-de7zhswl 225 23 indicator indicator NN cord-312431-de7zhswl 225 24 factors factor NNS cord-312431-de7zhswl 225 25 . . . cord-312431-de7zhswl 226 1 In in IN cord-312431-de7zhswl 226 2 this this DT cord-312431-de7zhswl 226 3 study study NN cord-312431-de7zhswl 226 4 , , , cord-312431-de7zhswl 226 5 TFF TFF NNP cord-312431-de7zhswl 226 6 with with IN cord-312431-de7zhswl 226 7 the the DT cord-312431-de7zhswl 226 8 Pellicon Pellicon NNP cord-312431-de7zhswl 226 9 Cassette Cassette NNP cord-312431-de7zhswl 226 10 System System NNP cord-312431-de7zhswl 226 11 ( ( -LRB- cord-312431-de7zhswl 226 12 Millipore Millipore NNP cord-312431-de7zhswl 226 13 ) ) -RRB- cord-312431-de7zhswl 226 14 for for IN cord-312431-de7zhswl 226 15 concentrating concentrate VBG cord-312431-de7zhswl 226 16 VLPs vlp NNS cord-312431-de7zhswl 226 17 from from IN cord-312431-de7zhswl 226 18 20 20 CD cord-312431-de7zhswl 226 19 L L NNP cord-312431-de7zhswl 226 20 river river NN cord-312431-de7zhswl 226 21 water water NN cord-312431-de7zhswl 226 22 samples sample NNS cord-312431-de7zhswl 226 23 was be VBD cord-312431-de7zhswl 226 24 used use VBN cord-312431-de7zhswl 226 25 . . . cord-312431-de7zhswl 227 1 The the DT cord-312431-de7zhswl 227 2 twostep twostep NN cord-312431-de7zhswl 227 3 filtration filtration NN cord-312431-de7zhswl 227 4 procedure procedure NN cord-312431-de7zhswl 227 5 used use VBN cord-312431-de7zhswl 227 6 in in IN cord-312431-de7zhswl 227 7 our -PRON- PRP$ cord-312431-de7zhswl 227 8 laboratory laboratory NN cord-312431-de7zhswl 227 9 involved involve VBD cord-312431-de7zhswl 227 10 fluid fluid NN cord-312431-de7zhswl 227 11 flowing flow VBG cord-312431-de7zhswl 227 12 parallel parallel NN cord-312431-de7zhswl 227 13 to to IN cord-312431-de7zhswl 227 14 the the DT cord-312431-de7zhswl 227 15 0.22 0.22 CD cord-312431-de7zhswl 227 16 mm mm NN cord-312431-de7zhswl 227 17 TFF TFF NNP cord-312431-de7zhswl 227 18 filter filter NN cord-312431-de7zhswl 227 19 surface surface NN cord-312431-de7zhswl 227 20 . . . cord-312431-de7zhswl 228 1 Particles particle NNS cord-312431-de7zhswl 228 2 with with IN cord-312431-de7zhswl 228 3 molecular molecular JJ cord-312431-de7zhswl 228 4 weights weight NNS cord-312431-de7zhswl 228 5 ( ( -LRB- cord-312431-de7zhswl 228 6 M M NNP cord-312431-de7zhswl 228 7 w w NNP cord-312431-de7zhswl 228 8 ) ) -RRB- cord-312431-de7zhswl 228 9 smaller small JJR cord-312431-de7zhswl 228 10 than than IN cord-312431-de7zhswl 228 11 the the DT cord-312431-de7zhswl 228 12 exclusion exclusion NN cord-312431-de7zhswl 228 13 size size NN cord-312431-de7zhswl 228 14 ( ( -LRB- cord-312431-de7zhswl 228 15 0.22 0.22 CD cord-312431-de7zhswl 228 16 mm mm NNP cord-312431-de7zhswl 228 17 ) ) -RRB- cord-312431-de7zhswl 228 18 of of IN cord-312431-de7zhswl 228 19 the the DT cord-312431-de7zhswl 228 20 filter filter NN cord-312431-de7zhswl 228 21 passed pass VBN cord-312431-de7zhswl 228 22 through through RP cord-312431-de7zhswl 228 23 and and CC cord-312431-de7zhswl 228 24 collected collect VBN cord-312431-de7zhswl 228 25 ( ( -LRB- cord-312431-de7zhswl 228 26 filtrate filtrate NN cord-312431-de7zhswl 228 27 ) ) -RRB- cord-312431-de7zhswl 228 28 in in IN cord-312431-de7zhswl 228 29 a a DT cord-312431-de7zhswl 228 30 reservoir reservoir NN cord-312431-de7zhswl 228 31 which which WDT cord-312431-de7zhswl 228 32 was be VBD cord-312431-de7zhswl 228 33 further further RB cord-312431-de7zhswl 228 34 concentrated concentrate VBN cord-312431-de7zhswl 228 35 through through IN cord-312431-de7zhswl 228 36 a a DT cord-312431-de7zhswl 228 37 100 100 CD cord-312431-de7zhswl 228 38 kDa kDa NNP cord-312431-de7zhswl 228 39 spiral spiral NN cord-312431-de7zhswl 228 40 wound wind VBN cord-312431-de7zhswl 228 41 TFF TFF NNP cord-312431-de7zhswl 228 42 unit unit NN cord-312431-de7zhswl 228 43 . . . cord-312431-de7zhswl 229 1 Particles particle NNS cord-312431-de7zhswl 229 2 with with IN cord-312431-de7zhswl 229 3 larger large JJR cord-312431-de7zhswl 229 4 molecular molecular JJ cord-312431-de7zhswl 229 5 weights weight NNS cord-312431-de7zhswl 229 6 were be VBD cord-312431-de7zhswl 229 7 retained retain VBN cord-312431-de7zhswl 229 8 and and CC cord-312431-de7zhswl 229 9 recycled recycle VBN cord-312431-de7zhswl 229 10 to to IN cord-312431-de7zhswl 229 11 the the DT cord-312431-de7zhswl 229 12 original original JJ cord-312431-de7zhswl 229 13 reservoir reservoir NN cord-312431-de7zhswl 229 14 ( ( -LRB- cord-312431-de7zhswl 229 15 retentate retentate NNP cord-312431-de7zhswl 229 16 ) ) -RRB- cord-312431-de7zhswl 229 17 . . . cord-312431-de7zhswl 230 1 Thus thus RB cord-312431-de7zhswl 230 2 , , , cord-312431-de7zhswl 230 3 as as IN cord-312431-de7zhswl 230 4 the the DT cord-312431-de7zhswl 230 5 filtration filtration NN cord-312431-de7zhswl 230 6 continued continue VBD cord-312431-de7zhswl 230 7 , , , cord-312431-de7zhswl 230 8 the the DT cord-312431-de7zhswl 230 9 retentate retentate NN cord-312431-de7zhswl 230 10 volume volume NN cord-312431-de7zhswl 230 11 decreased decrease VBD cord-312431-de7zhswl 230 12 until until IN cord-312431-de7zhswl 230 13 the the DT cord-312431-de7zhswl 230 14 desired desire VBN cord-312431-de7zhswl 230 15 volume volume NN cord-312431-de7zhswl 230 16 was be VBD cord-312431-de7zhswl 230 17 attained attain VBN cord-312431-de7zhswl 230 18 . . . cord-312431-de7zhswl 231 1 The the DT cord-312431-de7zhswl 231 2 TEM TEM NNP cord-312431-de7zhswl 231 3 study study NN cord-312431-de7zhswl 231 4 demonstrated demonstrate VBD cord-312431-de7zhswl 231 5 that that IN cord-312431-de7zhswl 231 6 the the DT cord-312431-de7zhswl 231 7 two two CD cord-312431-de7zhswl 231 8 - - HYPH cord-312431-de7zhswl 231 9 step step NN cord-312431-de7zhswl 231 10 TFF tff NN cord-312431-de7zhswl 231 11 procedure procedure NN cord-312431-de7zhswl 231 12 coupled couple VBN cord-312431-de7zhswl 231 13 with with IN cord-312431-de7zhswl 231 14 ultracentrifugation ultracentrifugation NN cord-312431-de7zhswl 231 15 produced produce VBD cord-312431-de7zhswl 231 16 a a DT cord-312431-de7zhswl 231 17 viral viral JJ cord-312431-de7zhswl 231 18 concentrate concentrate NN cord-312431-de7zhswl 231 19 devoid devoid JJ cord-312431-de7zhswl 231 20 of of IN cord-312431-de7zhswl 231 21 bacteria bacteria NNS cord-312431-de7zhswl 231 22 , , , cord-312431-de7zhswl 231 23 with with IN cord-312431-de7zhswl 231 24 most most JJS cord-312431-de7zhswl 231 25 viruses virus NNS cord-312431-de7zhswl 231 26 being be VBG cord-312431-de7zhswl 231 27 intact intact JJ cord-312431-de7zhswl 231 28 . . . cord-312431-de7zhswl 232 1 The the DT cord-312431-de7zhswl 232 2 phage phage NN cord-312431-de7zhswl 232 3 / / SYM cord-312431-de7zhswl 232 4 VLPs vlp NNS cord-312431-de7zhswl 232 5 concentrations concentration NNS cord-312431-de7zhswl 232 6 in in IN cord-312431-de7zhswl 232 7 every every DT cord-312431-de7zhswl 232 8 sample sample NN cord-312431-de7zhswl 232 9 were be VBD cord-312431-de7zhswl 232 10 high high JJ cord-312431-de7zhswl 232 11 enough enough RB cord-312431-de7zhswl 232 12 to to TO cord-312431-de7zhswl 232 13 view view VB cord-312431-de7zhswl 232 14 on on IN cord-312431-de7zhswl 232 15 formvar formvar NNP cord-312431-de7zhswl 232 16 coated coat VBN cord-312431-de7zhswl 232 17 electron electron NN cord-312431-de7zhswl 232 18 microscopic microscopic JJ cord-312431-de7zhswl 232 19 grids grid NNS cord-312431-de7zhswl 232 20 without without IN cord-312431-de7zhswl 232 21 enrichment enrichment NN cord-312431-de7zhswl 232 22 . . . cord-312431-de7zhswl 233 1 All all DT cord-312431-de7zhswl 233 2 water water NN cord-312431-de7zhswl 233 3 samples sample NNS cord-312431-de7zhswl 233 4 in in IN cord-312431-de7zhswl 233 5 this this DT cord-312431-de7zhswl 233 6 study study NN cord-312431-de7zhswl 233 7 contained contain VBD cord-312431-de7zhswl 233 8 a a DT cord-312431-de7zhswl 233 9 mixture mixture NN cord-312431-de7zhswl 233 10 of of IN cord-312431-de7zhswl 233 11 morphologically morphologically RB cord-312431-de7zhswl 233 12 different different JJ cord-312431-de7zhswl 233 13 tailed tailed JJ cord-312431-de7zhswl 233 14 phage phage NN cord-312431-de7zhswl 233 15 viruses virus NNS cord-312431-de7zhswl 233 16 , , , cord-312431-de7zhswl 233 17 which which WDT cord-312431-de7zhswl 233 18 were be VBD cord-312431-de7zhswl 233 19 regarded regard VBN cord-312431-de7zhswl 233 20 as as IN cord-312431-de7zhswl 233 21 bacteriophages bacteriophage NNS cord-312431-de7zhswl 233 22 . . . cord-312431-de7zhswl 234 1 It -PRON- PRP cord-312431-de7zhswl 234 2 was be VBD cord-312431-de7zhswl 234 3 possible possible JJ cord-312431-de7zhswl 234 4 to to TO cord-312431-de7zhswl 234 5 observe observe VB cord-312431-de7zhswl 234 6 viral viral JJ cord-312431-de7zhswl 234 7 particles particle NNS cord-312431-de7zhswl 234 8 with with IN cord-312431-de7zhswl 234 9 long long JJ cord-312431-de7zhswl 234 10 tails tail NNS cord-312431-de7zhswl 234 11 ( ( -LRB- cord-312431-de7zhswl 234 12 B1-Siphoviridae b1-siphoviridae ADD cord-312431-de7zhswl 234 13 ) ) -RRB- cord-312431-de7zhswl 234 14 , , , cord-312431-de7zhswl 234 15 short short JJ cord-312431-de7zhswl 234 16 tails tail NNS cord-312431-de7zhswl 234 17 ( ( -LRB- cord-312431-de7zhswl 234 18 A1-Myoviridae A1-Myoviridae NNP cord-312431-de7zhswl 234 19 and and CC cord-312431-de7zhswl 234 20 C1-Podoviridae C1-Podoviridae NNP cord-312431-de7zhswl 234 21 ) ) -RRB- cord-312431-de7zhswl 234 22 , , , cord-312431-de7zhswl 234 23 and and CC cord-312431-de7zhswl 234 24 without without IN cord-312431-de7zhswl 234 25 tails tail NNS cord-312431-de7zhswl 234 26 . . . cord-312431-de7zhswl 235 1 The the DT cord-312431-de7zhswl 235 2 phages phage NNS cord-312431-de7zhswl 235 3 particles particle NNS cord-312431-de7zhswl 235 4 observed observe VBN cord-312431-de7zhswl 235 5 without without IN cord-312431-de7zhswl 235 6 tails tail NNS cord-312431-de7zhswl 235 7 may may MD cord-312431-de7zhswl 235 8 belong belong VB cord-312431-de7zhswl 235 9 to to IN cord-312431-de7zhswl 235 10 a a DT cord-312431-de7zhswl 235 11 wide wide JJ cord-312431-de7zhswl 235 12 range range NN cord-312431-de7zhswl 235 13 of of IN cord-312431-de7zhswl 235 14 hosts host NNS cord-312431-de7zhswl 235 15 , , , cord-312431-de7zhswl 235 16 including include VBG cord-312431-de7zhswl 235 17 eukaryotes eukaryote NNS cord-312431-de7zhswl 235 18 [ [ -LRB- cord-312431-de7zhswl 235 19 66 66 CD cord-312431-de7zhswl 235 20 ] ] -RRB- cord-312431-de7zhswl 235 21 . . . cord-312431-de7zhswl 236 1 Most Most JJS cord-312431-de7zhswl 236 2 of of IN cord-312431-de7zhswl 236 3 the the DT cord-312431-de7zhswl 236 4 phages phage NNS cord-312431-de7zhswl 236 5 from from IN cord-312431-de7zhswl 236 6 the the DT cord-312431-de7zhswl 236 7 Umgeni Umgeni NNP cord-312431-de7zhswl 236 8 River River NNP cord-312431-de7zhswl 236 9 were be VBD cord-312431-de7zhswl 236 10 intact intact JJ cord-312431-de7zhswl 236 11 , , , cord-312431-de7zhswl 236 12 with with IN cord-312431-de7zhswl 236 13 discrete discrete JJ cord-312431-de7zhswl 236 14 structures structure NNS cord-312431-de7zhswl 236 15 such such JJ cord-312431-de7zhswl 236 16 as as IN cord-312431-de7zhswl 236 17 tail tail NN cord-312431-de7zhswl 236 18 fibres fibre NNS cord-312431-de7zhswl 236 19 , , , cord-312431-de7zhswl 236 20 base base NN cord-312431-de7zhswl 236 21 plates plate NNS cord-312431-de7zhswl 236 22 , , , cord-312431-de7zhswl 236 23 and and CC cord-312431-de7zhswl 236 24 other other JJ cord-312431-de7zhswl 236 25 appendages appendage NNS cord-312431-de7zhswl 236 26 which which WDT cord-312431-de7zhswl 236 27 are be VBP cord-312431-de7zhswl 236 28 pertinent pertinent JJ cord-312431-de7zhswl 236 29 for for IN cord-312431-de7zhswl 236 30 the the DT cord-312431-de7zhswl 236 31 recognition recognition NN cord-312431-de7zhswl 236 32 of of IN cord-312431-de7zhswl 236 33 and and CC cord-312431-de7zhswl 236 34 interaction interaction NN cord-312431-de7zhswl 236 35 with with IN cord-312431-de7zhswl 236 36 the the DT cord-312431-de7zhswl 236 37 host host NN cord-312431-de7zhswl 236 38 cell cell NN cord-312431-de7zhswl 236 39 . . . cord-312431-de7zhswl 237 1 These these DT cord-312431-de7zhswl 237 2 accessories accessory NNS cord-312431-de7zhswl 237 3 may may MD cord-312431-de7zhswl 237 4 be be VB cord-312431-de7zhswl 237 5 an an DT cord-312431-de7zhswl 237 6 indication indication NN cord-312431-de7zhswl 237 7 that that IN cord-312431-de7zhswl 237 8 a a DT cord-312431-de7zhswl 237 9 significant significant JJ cord-312431-de7zhswl 237 10 proportion proportion NN cord-312431-de7zhswl 237 11 of of IN cord-312431-de7zhswl 237 12 the the DT cord-312431-de7zhswl 237 13 phages phage NNS cord-312431-de7zhswl 237 14 are be VBP cord-312431-de7zhswl 237 15 suspended suspend VBN cord-312431-de7zhswl 237 16 in in IN cord-312431-de7zhswl 237 17 the the DT cord-312431-de7zhswl 237 18 water water NN cord-312431-de7zhswl 237 19 environment environment NN cord-312431-de7zhswl 237 20 as as IN cord-312431-de7zhswl 237 21 potentially potentially RB cord-312431-de7zhswl 237 22 infective infective JJ cord-312431-de7zhswl 237 23 particles particle NNS cord-312431-de7zhswl 237 24 . . . cord-312431-de7zhswl 238 1 The the DT cord-312431-de7zhswl 238 2 main main JJ cord-312431-de7zhswl 238 3 purposes purpose NNS cord-312431-de7zhswl 238 4 of of IN cord-312431-de7zhswl 238 5 phage phage NN cord-312431-de7zhswl 238 6 classification classification NN cord-312431-de7zhswl 238 7 are be VBP cord-312431-de7zhswl 238 8 generalisation generalisation NN cord-312431-de7zhswl 238 9 and and CC cord-312431-de7zhswl 238 10 simplification simplification NN cord-312431-de7zhswl 238 11 , , , cord-312431-de7zhswl 238 12 which which WDT cord-312431-de7zhswl 238 13 facilitates facilitate VBZ cord-312431-de7zhswl 238 14 comparisons comparison NNS cord-312431-de7zhswl 238 15 and and CC cord-312431-de7zhswl 238 16 understanding understanding NN cord-312431-de7zhswl 238 17 of of IN cord-312431-de7zhswl 238 18 viruses virus NNS cord-312431-de7zhswl 238 19 [ [ -LRB- cord-312431-de7zhswl 238 20 67 67 CD cord-312431-de7zhswl 238 21 ] ] -RRB- cord-312431-de7zhswl 238 22 . . . cord-312431-de7zhswl 239 1 The the DT cord-312431-de7zhswl 239 2 high high JJ cord-312431-de7zhswl 239 3 morphological morphological JJ cord-312431-de7zhswl 239 4 diversity diversity NN cord-312431-de7zhswl 239 5 of of IN cord-312431-de7zhswl 239 6 phage phage NN cord-312431-de7zhswl 239 7 communities community NNS cord-312431-de7zhswl 239 8 corresponds correspond VBZ cord-312431-de7zhswl 239 9 well well RB cord-312431-de7zhswl 239 10 with with IN cord-312431-de7zhswl 239 11 the the DT cord-312431-de7zhswl 239 12 great great JJ cord-312431-de7zhswl 239 13 variability variability NN cord-312431-de7zhswl 239 14 and and CC cord-312431-de7zhswl 239 15 dynamics dynamic NNS cord-312431-de7zhswl 239 16 of of IN cord-312431-de7zhswl 239 17 bacterial bacterial JJ cord-312431-de7zhswl 239 18 populations population NNS cord-312431-de7zhswl 239 19 obtained obtain VBN cord-312431-de7zhswl 239 20 in in IN cord-312431-de7zhswl 239 21 this this DT cord-312431-de7zhswl 239 22 current current JJ cord-312431-de7zhswl 239 23 study study NN cord-312431-de7zhswl 239 24 . . . cord-312431-de7zhswl 240 1 Electron electron NN cord-312431-de7zhswl 240 2 microscopy microscopy NN cord-312431-de7zhswl 240 3 provides provide VBZ cord-312431-de7zhswl 240 4 a a DT cord-312431-de7zhswl 240 5 direct direct JJ cord-312431-de7zhswl 240 6 insight insight NN cord-312431-de7zhswl 240 7 into into IN cord-312431-de7zhswl 240 8 the the DT cord-312431-de7zhswl 240 9 morphological morphological JJ cord-312431-de7zhswl 240 10 variability variability NN cord-312431-de7zhswl 240 11 of of IN cord-312431-de7zhswl 240 12 phage phage NN cord-312431-de7zhswl 240 13 present present JJ cord-312431-de7zhswl 240 14 in in IN cord-312431-de7zhswl 240 15 the the DT cord-312431-de7zhswl 240 16 water water NN cord-312431-de7zhswl 240 17 environment environment NN cord-312431-de7zhswl 240 18 , , , cord-312431-de7zhswl 240 19 without without IN cord-312431-de7zhswl 240 20 being be VBG cord-312431-de7zhswl 240 21 dependent dependent JJ cord-312431-de7zhswl 240 22 on on IN cord-312431-de7zhswl 240 23 the the DT cord-312431-de7zhswl 240 24 isolation isolation NN cord-312431-de7zhswl 240 25 of of IN cord-312431-de7zhswl 240 26 suitable suitable JJ cord-312431-de7zhswl 240 27 host host NN cord-312431-de7zhswl 240 28 strains strain NNS cord-312431-de7zhswl 240 29 . . . cord-312431-de7zhswl 241 1 This this DT cord-312431-de7zhswl 241 2 is be VBZ cord-312431-de7zhswl 241 3 important important JJ cord-312431-de7zhswl 241 4 , , , cord-312431-de7zhswl 241 5 as as IN cord-312431-de7zhswl 241 6 bacteria bacteria NNS cord-312431-de7zhswl 241 7 which which WDT cord-312431-de7zhswl 241 8 are be VBP cord-312431-de7zhswl 241 9 not not RB cord-312431-de7zhswl 241 10 cultivable cultivable JJ cord-312431-de7zhswl 241 11 under under IN cord-312431-de7zhswl 241 12 laboratory laboratory NN cord-312431-de7zhswl 241 13 conditions condition NNS cord-312431-de7zhswl 241 14 usually usually RB cord-312431-de7zhswl 241 15 dominate dominate VBP cord-312431-de7zhswl 241 16 aquatic aquatic JJ cord-312431-de7zhswl 241 17 environments environment NNS cord-312431-de7zhswl 241 18 and and CC cord-312431-de7zhswl 241 19 may may MD cord-312431-de7zhswl 241 20 be be VB cord-312431-de7zhswl 241 21 important important JJ cord-312431-de7zhswl 241 22 phage phage NN cord-312431-de7zhswl 241 23 hosts host NNS cord-312431-de7zhswl 241 24 [ [ -LRB- cord-312431-de7zhswl 241 25 68 68 CD cord-312431-de7zhswl 241 26 ] ] -RRB- cord-312431-de7zhswl 241 27 . . . cord-312431-de7zhswl 242 1 Great great JJ cord-312431-de7zhswl 242 2 morphological morphological JJ cord-312431-de7zhswl 242 3 variability variability NN cord-312431-de7zhswl 242 4 was be VBD cord-312431-de7zhswl 242 5 observed observe VBN cord-312431-de7zhswl 242 6 in in IN cord-312431-de7zhswl 242 7 the the DT cord-312431-de7zhswl 242 8 virus virus NN cord-312431-de7zhswl 242 9 assemblage assemblage NN cord-312431-de7zhswl 242 10 's be VBZ cord-312431-de7zhswl 242 11 from from IN cord-312431-de7zhswl 242 12 the the DT cord-312431-de7zhswl 242 13 Umgeni Umgeni NNP cord-312431-de7zhswl 242 14 River River NNP cord-312431-de7zhswl 242 15 , , , cord-312431-de7zhswl 242 16 which which WDT cord-312431-de7zhswl 242 17 suggests suggest VBZ cord-312431-de7zhswl 242 18 that that IN cord-312431-de7zhswl 242 19 these these DT cord-312431-de7zhswl 242 20 viruses virus NNS cord-312431-de7zhswl 242 21 may may MD cord-312431-de7zhswl 242 22 also also RB cord-312431-de7zhswl 242 23 be be VB cord-312431-de7zhswl 242 24 diverse diverse JJ cord-312431-de7zhswl 242 25 in in IN cord-312431-de7zhswl 242 26 terms term NNS cord-312431-de7zhswl 242 27 of of IN cord-312431-de7zhswl 242 28 the the DT cord-312431-de7zhswl 242 29 hosts host NNS cord-312431-de7zhswl 242 30 that that WDT cord-312431-de7zhswl 242 31 they -PRON- PRP cord-312431-de7zhswl 242 32 infect infect VBP cord-312431-de7zhswl 242 33 . . . cord-312431-de7zhswl 243 1 Most Most JJS cord-312431-de7zhswl 243 2 of of IN cord-312431-de7zhswl 243 3 the the DT cord-312431-de7zhswl 243 4 viruses virus NNS cord-312431-de7zhswl 243 5 were be VBD cord-312431-de7zhswl 243 6 detected detect VBN cord-312431-de7zhswl 243 7 downstream downstream RB cord-312431-de7zhswl 243 8 of of IN cord-312431-de7zhswl 243 9 the the DT cord-312431-de7zhswl 243 10 river river NN cord-312431-de7zhswl 243 11 towards towards IN cord-312431-de7zhswl 243 12 the the DT cord-312431-de7zhswl 243 13 river river NN cord-312431-de7zhswl 243 14 mouth mouth NN cord-312431-de7zhswl 243 15 area area NN cord-312431-de7zhswl 243 16 during during IN cord-312431-de7zhswl 243 17 the the DT cord-312431-de7zhswl 243 18 spring spring NN cord-312431-de7zhswl 243 19 and and CC cord-312431-de7zhswl 243 20 summer summer NN cord-312431-de7zhswl 243 21 seasons season NNS cord-312431-de7zhswl 243 22 . . . cord-312431-de7zhswl 244 1 The the DT cord-312431-de7zhswl 244 2 viral viral JJ cord-312431-de7zhswl 244 3 contamination contamination NN cord-312431-de7zhswl 244 4 of of IN cord-312431-de7zhswl 244 5 these these DT cord-312431-de7zhswl 244 6 widely widely RB cord-312431-de7zhswl 244 7 used use VBN cord-312431-de7zhswl 244 8 areas area NNS cord-312431-de7zhswl 244 9 could could MD cord-312431-de7zhswl 244 10 be be VB cord-312431-de7zhswl 244 11 attributed attribute VBN cord-312431-de7zhswl 244 12 to to IN cord-312431-de7zhswl 244 13 storm storm NN cord-312431-de7zhswl 244 14 water water NN cord-312431-de7zhswl 244 15 discharge discharge NN cord-312431-de7zhswl 244 16 , , , cord-312431-de7zhswl 244 17 surface surface NN cord-312431-de7zhswl 244 18 runoff runoff NN cord-312431-de7zhswl 244 19 , , , cord-312431-de7zhswl 244 20 sewage sewage NN cord-312431-de7zhswl 244 21 discharge discharge NN cord-312431-de7zhswl 244 22 and and CC cord-312431-de7zhswl 244 23 overflows overflow NNS cord-312431-de7zhswl 244 24 , , , cord-312431-de7zhswl 244 25 recreational recreational JJ cord-312431-de7zhswl 244 26 exposure exposure NN cord-312431-de7zhswl 244 27 and and CC cord-312431-de7zhswl 244 28 other other JJ cord-312431-de7zhswl 244 29 anthropogenic anthropogenic JJ cord-312431-de7zhswl 244 30 activities activity NNS cord-312431-de7zhswl 244 31 [ [ -LRB- cord-312431-de7zhswl 244 32 63 63 CD cord-312431-de7zhswl 244 33 ] ] -RRB- cord-312431-de7zhswl 244 34 . . . cord-312431-de7zhswl 245 1 The the DT cord-312431-de7zhswl 245 2 detection detection NN cord-312431-de7zhswl 245 3 of of IN cord-312431-de7zhswl 245 4 viruses virus NNS cord-312431-de7zhswl 245 5 by by IN cord-312431-de7zhswl 245 6 TEM tem NN cord-312431-de7zhswl 245 7 in in IN cord-312431-de7zhswl 245 8 the the DT cord-312431-de7zhswl 245 9 sampling sampling NN cord-312431-de7zhswl 245 10 points point NNS cord-312431-de7zhswl 245 11 upstream upstream RB cord-312431-de7zhswl 245 12 of of IN cord-312431-de7zhswl 245 13 the the DT cord-312431-de7zhswl 245 14 Umgeni Umgeni NNP cord-312431-de7zhswl 245 15 River River NNP cord-312431-de7zhswl 245 16 was be VBD cord-312431-de7zhswl 245 17 relatively relatively RB cord-312431-de7zhswl 245 18 low low JJ cord-312431-de7zhswl 245 19 or or CC cord-312431-de7zhswl 245 20 nonexistent nonexistent JJ cord-312431-de7zhswl 245 21 , , , cord-312431-de7zhswl 245 22 probably probably RB cord-312431-de7zhswl 245 23 due due IN cord-312431-de7zhswl 245 24 to to IN cord-312431-de7zhswl 245 25 the the DT cord-312431-de7zhswl 245 26 stagnant stagnant JJ cord-312431-de7zhswl 245 27 waters water NNS cord-312431-de7zhswl 245 28 and and CC cord-312431-de7zhswl 245 29 limited limited JJ cord-312431-de7zhswl 245 30 recreational recreational JJ cord-312431-de7zhswl 245 31 exposure exposure NN cord-312431-de7zhswl 245 32 . . . cord-312431-de7zhswl 246 1 Several several JJ cord-312431-de7zhswl 246 2 presumptive presumptive JJ cord-312431-de7zhswl 246 3 virus virus NN cord-312431-de7zhswl 246 4 types type NNS cord-312431-de7zhswl 246 5 including include VBG cord-312431-de7zhswl 246 6 Adenoviridae Adenoviridae NNP cord-312431-de7zhswl 246 7 , , , cord-312431-de7zhswl 246 8 Caliciviridae Caliciviridae NNP cord-312431-de7zhswl 246 9 ( ( -LRB- cord-312431-de7zhswl 246 10 norovirus norovirus NN cord-312431-de7zhswl 246 11 ) ) -RRB- cord-312431-de7zhswl 246 12 , , , cord-312431-de7zhswl 246 13 Coronaviridae Coronaviridae NNP cord-312431-de7zhswl 246 14 , , , cord-312431-de7zhswl 246 15 Herpesviridae Herpesviridae NNP cord-312431-de7zhswl 246 16 , , , cord-312431-de7zhswl 246 17 Orthomyxoviridae Orthomyxoviridae NNP cord-312431-de7zhswl 246 18 ( ( -LRB- cord-312431-de7zhswl 246 19 influenza influenza NN cord-312431-de7zhswl 246 20 virus virus NN cord-312431-de7zhswl 246 21 ) ) -RRB- cord-312431-de7zhswl 246 22 , , , cord-312431-de7zhswl 246 23 Picornaviridae Picornaviridae NNP cord-312431-de7zhswl 246 24 ( ( -LRB- cord-312431-de7zhswl 246 25 enterovirus enterovirus NN cord-312431-de7zhswl 246 26 ) ) -RRB- cord-312431-de7zhswl 246 27 , , , cord-312431-de7zhswl 246 28 Poxviridae Poxviridae NNP cord-312431-de7zhswl 246 29 and and CC cord-312431-de7zhswl 246 30 Reoviridae Reoviridae NNP cord-312431-de7zhswl 246 31 ( ( -LRB- cord-312431-de7zhswl 246 32 rotavirus rotavirus NN cord-312431-de7zhswl 246 33 ) ) -RRB- cord-312431-de7zhswl 246 34 were be VBD cord-312431-de7zhswl 246 35 found find VBN cord-312431-de7zhswl 246 36 in in IN cord-312431-de7zhswl 246 37 the the DT cord-312431-de7zhswl 246 38 Umgeni Umgeni NNP cord-312431-de7zhswl 246 39 River River NNP cord-312431-de7zhswl 246 40 during during IN cord-312431-de7zhswl 246 41 all all DT cord-312431-de7zhswl 246 42 seasons season NNS cord-312431-de7zhswl 246 43 based base VBN cord-312431-de7zhswl 246 44 on on IN cord-312431-de7zhswl 246 45 the the DT cord-312431-de7zhswl 246 46 morphologies morphology NNS cord-312431-de7zhswl 246 47 under under IN cord-312431-de7zhswl 246 48 TEM TEM NNP cord-312431-de7zhswl 246 49 . . . cord-312431-de7zhswl 247 1 Human human JJ cord-312431-de7zhswl 247 2 viruses virus NNS cord-312431-de7zhswl 247 3 seen see VBN cord-312431-de7zhswl 247 4 in in IN cord-312431-de7zhswl 247 5 negative negative JJ cord-312431-de7zhswl 247 6 stains stain NNS cord-312431-de7zhswl 247 7 fall fall VBP cord-312431-de7zhswl 247 8 into into IN cord-312431-de7zhswl 247 9 one one CD cord-312431-de7zhswl 247 10 of of IN cord-312431-de7zhswl 247 11 two two CD cord-312431-de7zhswl 247 12 major major JJ cord-312431-de7zhswl 247 13 morphological morphological JJ cord-312431-de7zhswl 247 14 categories category NNS cord-312431-de7zhswl 247 15 : : : cord-312431-de7zhswl 247 16 enveloped envelop VBN cord-312431-de7zhswl 247 17 or or CC cord-312431-de7zhswl 247 18 naked naked JJ cord-312431-de7zhswl 247 19 [ [ -LRB- cord-312431-de7zhswl 247 20 64 64 CD cord-312431-de7zhswl 247 21 ] ] -RRB- cord-312431-de7zhswl 247 22 . . . cord-312431-de7zhswl 248 1 Enveloped envelop VBN cord-312431-de7zhswl 248 2 viruses virus NNS cord-312431-de7zhswl 248 3 have have VBP cord-312431-de7zhswl 248 4 a a DT cord-312431-de7zhswl 248 5 nucleocapsid nucleocapsid NN cord-312431-de7zhswl 248 6 ( ( -LRB- cord-312431-de7zhswl 248 7 the the DT cord-312431-de7zhswl 248 8 nucleic nucleic JJ cord-312431-de7zhswl 248 9 acid acid NN cord-312431-de7zhswl 248 10 held hold VBN cord-312431-de7zhswl 248 11 together together RB cord-312431-de7zhswl 248 12 by by IN cord-312431-de7zhswl 248 13 some some DT cord-312431-de7zhswl 248 14 structural structural JJ cord-312431-de7zhswl 248 15 proteins protein NNS cord-312431-de7zhswl 248 16 ) ) -RRB- cord-312431-de7zhswl 248 17 inside inside RB cord-312431-de7zhswl 248 18 , , , cord-312431-de7zhswl 248 19 whilst whilst IN cord-312431-de7zhswl 248 20 naked naked JJ cord-312431-de7zhswl 248 21 viruses virus NNS cord-312431-de7zhswl 248 22 are be VBP cord-312431-de7zhswl 248 23 icosahedral icosahedral JJ cord-312431-de7zhswl 248 24 , , , cord-312431-de7zhswl 248 25 their -PRON- PRP$ cord-312431-de7zhswl 248 26 protein protein NN cord-312431-de7zhswl 248 27 coat coat NN cord-312431-de7zhswl 248 28 or or CC cord-312431-de7zhswl 248 29 capsid capsid NN cord-312431-de7zhswl 248 30 is be VBZ cord-312431-de7zhswl 248 31 more more RBR cord-312431-de7zhswl 248 32 rigid rigid JJ cord-312431-de7zhswl 248 33 and and CC cord-312431-de7zhswl 248 34 withstands withstand VBZ cord-312431-de7zhswl 248 35 the the DT cord-312431-de7zhswl 248 36 drying drying NN cord-312431-de7zhswl 248 37 process process NN cord-312431-de7zhswl 248 38 well well RB cord-312431-de7zhswl 248 39 to to TO cord-312431-de7zhswl 248 40 maintain maintain VB cord-312431-de7zhswl 248 41 their -PRON- PRP$ cord-312431-de7zhswl 248 42 spherical spherical JJ cord-312431-de7zhswl 248 43 structure structure NN cord-312431-de7zhswl 248 44 in in IN cord-312431-de7zhswl 248 45 negative negative JJ cord-312431-de7zhswl 248 46 stains stain NNS cord-312431-de7zhswl 248 47 [ [ -LRB- cord-312431-de7zhswl 248 48 50 50 CD cord-312431-de7zhswl 248 49 ] ] -RRB- cord-312431-de7zhswl 248 50 . . . cord-312431-de7zhswl 249 1 Naked naked JJ cord-312431-de7zhswl 249 2 human human JJ cord-312431-de7zhswl 249 3 viruses virus NNS cord-312431-de7zhswl 249 4 are be VBP cord-312431-de7zhswl 249 5 of of IN cord-312431-de7zhswl 249 6 three three CD cord-312431-de7zhswl 249 7 size size NN cord-312431-de7zhswl 249 8 ranges range NNS cord-312431-de7zhswl 249 9 : : : cord-312431-de7zhswl 249 10 ( ( -LRB- cord-312431-de7zhswl 249 11 i i NN cord-312431-de7zhswl 249 12 ) ) -RRB- cord-312431-de7zhswl 249 13 22 22 CD cord-312431-de7zhswl 249 14 - - SYM cord-312431-de7zhswl 249 15 35 35 CD cord-312431-de7zhswl 249 16 nm nm NN cord-312431-de7zhswl 249 17 ( ( -LRB- cord-312431-de7zhswl 249 18 e.g. e.g. IN cord-312431-de7zhswl 249 19 parvoviruses parvoviruses NNPS cord-312431-de7zhswl 249 20 , , , cord-312431-de7zhswl 249 21 enteroviruses enteroviruses NNP cord-312431-de7zhswl 249 22 and and CC cord-312431-de7zhswl 249 23 caliciviruses caliciviruses NNP cord-312431-de7zhswl 249 24 ) ) -RRB- cord-312431-de7zhswl 249 25 , , , cord-312431-de7zhswl 249 26 ( ( -LRB- cord-312431-de7zhswl 249 27 ii ii NN cord-312431-de7zhswl 249 28 ) ) -RRB- cord-312431-de7zhswl 249 29 40 40 CD cord-312431-de7zhswl 249 30 - - SYM cord-312431-de7zhswl 249 31 55 55 CD cord-312431-de7zhswl 249 32 nm nm NN cord-312431-de7zhswl 249 33 ( ( -LRB- cord-312431-de7zhswl 249 34 polyomaviruses polyomaviruse NNS cord-312431-de7zhswl 249 35 and and CC cord-312431-de7zhswl 249 36 papillomaviruses papillomaviruse NNS cord-312431-de7zhswl 249 37 ) ) -RRB- cord-312431-de7zhswl 249 38 and and CC cord-312431-de7zhswl 249 39 ( ( -LRB- cord-312431-de7zhswl 249 40 iii iii NN cord-312431-de7zhswl 249 41 ) ) -RRB- cord-312431-de7zhswl 249 42 70 70 CD cord-312431-de7zhswl 249 43 Â â CD cord-312431-de7zhswl 249 44 90 90 CD cord-312431-de7zhswl 249 45 nm nm NN cord-312431-de7zhswl 250 1 ( ( -LRB- cord-312431-de7zhswl 250 2 reoviruses reoviruses NNP cord-312431-de7zhswl 250 3 , , , cord-312431-de7zhswl 250 4 rotaviruses rotaviruses NNP cord-312431-de7zhswl 250 5 and and CC cord-312431-de7zhswl 250 6 adenoviruses adenovirus NNS cord-312431-de7zhswl 250 7 ) ) -RRB- cord-312431-de7zhswl 250 8 [ [ -LRB- cord-312431-de7zhswl 250 9 69 69 CD cord-312431-de7zhswl 250 10 ] ] -RRB- cord-312431-de7zhswl 250 11 . . . cord-312431-de7zhswl 251 1 As as IN cord-312431-de7zhswl 251 2 a a DT cord-312431-de7zhswl 251 3 general general JJ cord-312431-de7zhswl 251 4 pattern pattern NN cord-312431-de7zhswl 251 5 , , , cord-312431-de7zhswl 251 6 the the DT cord-312431-de7zhswl 251 7 virus virus NN cord-312431-de7zhswl 251 8 population population NN cord-312431-de7zhswl 251 9 was be VBD cord-312431-de7zhswl 251 10 dominated dominate VBN cord-312431-de7zhswl 251 11 by by IN cord-312431-de7zhswl 251 12 small small JJ cord-312431-de7zhswl 251 13 forms form NNS cord-312431-de7zhswl 251 14 ( ( -LRB- cord-312431-de7zhswl 251 15 < < XX cord-312431-de7zhswl 251 16 40 40 CD cord-312431-de7zhswl 251 17 nm nm CD cord-312431-de7zhswl 251 18 ) ) -RRB- cord-312431-de7zhswl 251 19 , , , cord-312431-de7zhswl 251 20 and and CC cord-312431-de7zhswl 251 21 tails tail NNS cord-312431-de7zhswl 251 22 were be VBD cord-312431-de7zhswl 251 23 rarely rarely RB cord-312431-de7zhswl 251 24 seen see VBN cord-312431-de7zhswl 251 25 , , , cord-312431-de7zhswl 251 26 which which WDT cord-312431-de7zhswl 251 27 is be VBZ cord-312431-de7zhswl 251 28 in in IN cord-312431-de7zhswl 251 29 agreement agreement NN cord-312431-de7zhswl 251 30 with with IN cord-312431-de7zhswl 251 31 the the DT cord-312431-de7zhswl 251 32 results result NNS cord-312431-de7zhswl 251 33 reported report VBN cord-312431-de7zhswl 251 34 by by IN cord-312431-de7zhswl 251 35 several several JJ cord-312431-de7zhswl 251 36 authors author NNS cord-312431-de7zhswl 251 37 [ [ -LRB- cord-312431-de7zhswl 251 38 66 66 CD cord-312431-de7zhswl 251 39 , , , cord-312431-de7zhswl 251 40 [ [ -LRB- cord-312431-de7zhswl 251 41 70 70 CD cord-312431-de7zhswl 251 42 ] ] -RRB- cord-312431-de7zhswl 251 43 [ [ -LRB- cord-312431-de7zhswl 251 44 71 71 CD cord-312431-de7zhswl 251 45 ] ] -RRB- cord-312431-de7zhswl 251 46 [ [ -LRB- cord-312431-de7zhswl 251 47 72 72 CD cord-312431-de7zhswl 251 48 ] ] -RRB- cord-312431-de7zhswl 251 49 . . . cord-312431-de7zhswl 252 1 The the DT cord-312431-de7zhswl 252 2 negative negative JJ cord-312431-de7zhswl 252 3 staining staining NN cord-312431-de7zhswl 252 4 technique technique NN cord-312431-de7zhswl 252 5 for for IN cord-312431-de7zhswl 252 6 TEM tem NN cord-312431-de7zhswl 252 7 allowed allow VBN cord-312431-de7zhswl 252 8 for for IN cord-312431-de7zhswl 252 9 the the DT cord-312431-de7zhswl 252 10 examination examination NN cord-312431-de7zhswl 252 11 of of IN cord-312431-de7zhswl 252 12 particulate particulate NN cord-312431-de7zhswl 252 13 material material NN cord-312431-de7zhswl 252 14 including include VBG cord-312431-de7zhswl 252 15 determination determination NN cord-312431-de7zhswl 252 16 of of IN cord-312431-de7zhswl 252 17 structure structure NN cord-312431-de7zhswl 252 18 and and CC cord-312431-de7zhswl 252 19 size size NN cord-312431-de7zhswl 252 20 of of IN cord-312431-de7zhswl 252 21 particles particle NNS cord-312431-de7zhswl 252 22 and and CC cord-312431-de7zhswl 252 23 has have VBZ cord-312431-de7zhswl 252 24 proved prove VBN cord-312431-de7zhswl 252 25 important important JJ cord-312431-de7zhswl 252 26 in in IN cord-312431-de7zhswl 252 27 virological virological JJ cord-312431-de7zhswl 252 28 studies study NNS cord-312431-de7zhswl 252 29 [ [ -LRB- cord-312431-de7zhswl 252 30 50 50 CD cord-312431-de7zhswl 252 31 ] ] -RRB- cord-312431-de7zhswl 252 32 . . . cord-312431-de7zhswl 253 1 All all DT cord-312431-de7zhswl 253 2 water water NN cord-312431-de7zhswl 253 3 samples sample NNS cord-312431-de7zhswl 253 4 analysed analyse VBN cord-312431-de7zhswl 253 5 were be VBD cord-312431-de7zhswl 253 6 environmental environmental JJ cord-312431-de7zhswl 253 7 in in IN cord-312431-de7zhswl 253 8 nature nature NN cord-312431-de7zhswl 253 9 , , , cord-312431-de7zhswl 253 10 thus thus RB cord-312431-de7zhswl 253 11 natural natural JJ cord-312431-de7zhswl 253 12 degradation degradation NN cord-312431-de7zhswl 253 13 may may MD cord-312431-de7zhswl 253 14 have have VB cord-312431-de7zhswl 253 15 altered alter VBN cord-312431-de7zhswl 253 16 the the DT cord-312431-de7zhswl 253 17 morphological morphological JJ cord-312431-de7zhswl 253 18 features feature NNS cord-312431-de7zhswl 253 19 of of IN cord-312431-de7zhswl 253 20 the the DT cord-312431-de7zhswl 253 21 viruses virus NNS cord-312431-de7zhswl 253 22 substantially substantially RB cord-312431-de7zhswl 253 23 [ [ -LRB- cord-312431-de7zhswl 253 24 50 50 CD cord-312431-de7zhswl 253 25 ] ] -RRB- cord-312431-de7zhswl 253 26 . . . cord-312431-de7zhswl 254 1 The the DT cord-312431-de7zhswl 254 2 application application NN cord-312431-de7zhswl 254 3 of of IN cord-312431-de7zhswl 254 4 PCR PCR NNP cord-312431-de7zhswl 254 5 - - HYPH cord-312431-de7zhswl 254 6 based base VBN cord-312431-de7zhswl 254 7 molecular molecular JJ cord-312431-de7zhswl 254 8 technology technology NN cord-312431-de7zhswl 254 9 and and CC cord-312431-de7zhswl 254 10 TEM TEM NNP cord-312431-de7zhswl 254 11 has have VBZ cord-312431-de7zhswl 254 12 advanced advance VBN cord-312431-de7zhswl 254 13 our -PRON- PRP$ cord-312431-de7zhswl 254 14 knowledge knowledge NN cord-312431-de7zhswl 254 15 of of IN cord-312431-de7zhswl 254 16 the the DT cord-312431-de7zhswl 254 17 occurrence occurrence NN cord-312431-de7zhswl 254 18 and and CC cord-312431-de7zhswl 254 19 prevalence prevalence NN cord-312431-de7zhswl 254 20 of of IN cord-312431-de7zhswl 254 21 human human JJ cord-312431-de7zhswl 254 22 viruses virus NNS cord-312431-de7zhswl 254 23 in in IN cord-312431-de7zhswl 254 24 water water NN cord-312431-de7zhswl 254 25 . . . cord-312431-de7zhswl 255 1 However however RB cord-312431-de7zhswl 255 2 , , , cord-312431-de7zhswl 255 3 it -PRON- PRP cord-312431-de7zhswl 255 4 has have VBZ cord-312431-de7zhswl 255 5 provided provide VBN cord-312431-de7zhswl 255 6 no no DT cord-312431-de7zhswl 255 7 information information NN cord-312431-de7zhswl 255 8 on on IN cord-312431-de7zhswl 255 9 viral viral JJ cord-312431-de7zhswl 255 10 viability viability NN cord-312431-de7zhswl 255 11 and and CC cord-312431-de7zhswl 255 12 infectivity infectivity NN cord-312431-de7zhswl 255 13 in in IN cord-312431-de7zhswl 255 14 a a DT cord-312431-de7zhswl 255 15 specific specific JJ cord-312431-de7zhswl 255 16 environment environment NN cord-312431-de7zhswl 255 17 . . . cord-312431-de7zhswl 256 1 The the DT cord-312431-de7zhswl 256 2 tissue tissue NN cord-312431-de7zhswl 256 3 culture culture NN cord-312431-de7zhswl 256 4 assay assay NN cord-312431-de7zhswl 256 5 is be VBZ cord-312431-de7zhswl 256 6 the the DT cord-312431-de7zhswl 256 7 only only JJ cord-312431-de7zhswl 256 8 US US NNP cord-312431-de7zhswl 256 9 EPA EPA NNP cord-312431-de7zhswl 256 10 approved approve VBN cord-312431-de7zhswl 256 11 method method NN cord-312431-de7zhswl 256 12 for for IN cord-312431-de7zhswl 256 13 virus virus NN cord-312431-de7zhswl 256 14 infectivity infectivity NN cord-312431-de7zhswl 256 15 monitoring monitor VBG cord-312431-de7zhswl 256 16 in in IN cord-312431-de7zhswl 256 17 aquatic aquatic JJ cord-312431-de7zhswl 256 18 samples sample NNS cord-312431-de7zhswl 256 19 [ [ -LRB- cord-312431-de7zhswl 256 20 73 73 CD cord-312431-de7zhswl 256 21 ] ] -RRB- cord-312431-de7zhswl 256 22 . . . cord-312431-de7zhswl 257 1 Virology virology NN cord-312431-de7zhswl 257 2 laboratories laboratory NNS cord-312431-de7zhswl 257 3 have have VBP cord-312431-de7zhswl 257 4 traditionally traditionally RB cord-312431-de7zhswl 257 5 used use VBN cord-312431-de7zhswl 257 6 tissue tissue NN cord-312431-de7zhswl 257 7 culture culture NN cord-312431-de7zhswl 257 8 cell cell NN cord-312431-de7zhswl 257 9 lines line NNS cord-312431-de7zhswl 257 10 to to TO cord-312431-de7zhswl 257 11 amplify amplify VB cord-312431-de7zhswl 257 12 the the DT cord-312431-de7zhswl 257 13 amount amount NN cord-312431-de7zhswl 257 14 of of IN cord-312431-de7zhswl 257 15 virus virus NN cord-312431-de7zhswl 257 16 present present JJ cord-312431-de7zhswl 257 17 in in IN cord-312431-de7zhswl 257 18 a a DT cord-312431-de7zhswl 257 19 water water NN cord-312431-de7zhswl 257 20 sample sample NN cord-312431-de7zhswl 257 21 , , , cord-312431-de7zhswl 257 22 express express VB cord-312431-de7zhswl 257 23 the the DT cord-312431-de7zhswl 257 24 viral viral JJ cord-312431-de7zhswl 257 25 antigens antigen NNS cord-312431-de7zhswl 257 26 and and CC cord-312431-de7zhswl 257 27 in in IN cord-312431-de7zhswl 257 28 many many JJ cord-312431-de7zhswl 257 29 cases case NNS cord-312431-de7zhswl 257 30 monitor monitor VBP cord-312431-de7zhswl 257 31 the the DT cord-312431-de7zhswl 257 32 cell cell NN cord-312431-de7zhswl 257 33 death death NN cord-312431-de7zhswl 257 34 as as IN cord-312431-de7zhswl 257 35 a a DT cord-312431-de7zhswl 257 36 consequence consequence NN cord-312431-de7zhswl 257 37 of of IN cord-312431-de7zhswl 257 38 the the DT cord-312431-de7zhswl 257 39 viral viral JJ cord-312431-de7zhswl 257 40 infection infection NN cord-312431-de7zhswl 257 41 producing produce VBG cord-312431-de7zhswl 257 42 characteristic characteristic JJ cord-312431-de7zhswl 257 43 CPEs cpe NNS cord-312431-de7zhswl 257 44 in in IN cord-312431-de7zhswl 257 45 the the DT cord-312431-de7zhswl 257 46 cell cell NN cord-312431-de7zhswl 257 47 monolayer monolayer NN cord-312431-de7zhswl 257 48 . . . cord-312431-de7zhswl 258 1 The the DT cord-312431-de7zhswl 258 2 tissue tissue NN cord-312431-de7zhswl 258 3 culture culture NN cord-312431-de7zhswl 258 4 technique technique NN cord-312431-de7zhswl 258 5 has have VBZ cord-312431-de7zhswl 258 6 been be VBN cord-312431-de7zhswl 258 7 used use VBN cord-312431-de7zhswl 258 8 extensively extensively RB cord-312431-de7zhswl 258 9 for for IN cord-312431-de7zhswl 258 10 virus virus NN cord-312431-de7zhswl 258 11 replication replication NN cord-312431-de7zhswl 258 12 and and CC cord-312431-de7zhswl 258 13 infectivity infectivity NN cord-312431-de7zhswl 258 14 studies study NNS cord-312431-de7zhswl 258 15 and and CC cord-312431-de7zhswl 258 16 plaque plaque NN cord-312431-de7zhswl 258 17 assays assay NNS cord-312431-de7zhswl 258 18 [ [ -LRB- cord-312431-de7zhswl 258 19 74 74 CD cord-312431-de7zhswl 258 20 ] ] -RRB- cord-312431-de7zhswl 258 21 . . . cord-312431-de7zhswl 259 1 It -PRON- PRP cord-312431-de7zhswl 259 2 has have VBZ cord-312431-de7zhswl 259 3 been be VBN cord-312431-de7zhswl 259 4 established establish VBN cord-312431-de7zhswl 259 5 that that IN cord-312431-de7zhswl 259 6 for for IN cord-312431-de7zhswl 259 7 the the DT cord-312431-de7zhswl 259 8 recovery recovery NN cord-312431-de7zhswl 259 9 of of IN cord-312431-de7zhswl 259 10 a a DT cord-312431-de7zhswl 259 11 maximum maximum JJ cord-312431-de7zhswl 259 12 number number NN cord-312431-de7zhswl 259 13 of of IN cord-312431-de7zhswl 259 14 infectious infectious JJ cord-312431-de7zhswl 259 15 viruses virus NNS cord-312431-de7zhswl 259 16 , , , cord-312431-de7zhswl 259 17 it -PRON- PRP cord-312431-de7zhswl 259 18 is be VBZ cord-312431-de7zhswl 259 19 often often RB cord-312431-de7zhswl 259 20 necessary necessary JJ cord-312431-de7zhswl 259 21 to to TO cord-312431-de7zhswl 259 22 inoculate inoculate VB cord-312431-de7zhswl 259 23 each each DT cord-312431-de7zhswl 259 24 sample sample NN cord-312431-de7zhswl 259 25 type type NN cord-312431-de7zhswl 259 26 into into IN cord-312431-de7zhswl 259 27 several several JJ cord-312431-de7zhswl 259 28 different different JJ cord-312431-de7zhswl 259 29 cell cell NN cord-312431-de7zhswl 259 30 culture culture NN cord-312431-de7zhswl 259 31 lines line NNS cord-312431-de7zhswl 259 32 because because IN cord-312431-de7zhswl 259 33 no no DT cord-312431-de7zhswl 259 34 single single JJ cord-312431-de7zhswl 259 35 cell cell NN cord-312431-de7zhswl 259 36 culture culture NN cord-312431-de7zhswl 259 37 is be VBZ cord-312431-de7zhswl 259 38 available available JJ cord-312431-de7zhswl 259 39 with with IN cord-312431-de7zhswl 259 40 susceptibility susceptibility NN cord-312431-de7zhswl 259 41 to to IN cord-312431-de7zhswl 259 42 all all DT cord-312431-de7zhswl 259 43 enteric enteric JJ cord-312431-de7zhswl 259 44 virus virus NN cord-312431-de7zhswl 259 45 types type NNS cord-312431-de7zhswl 259 46 [ [ -LRB- cord-312431-de7zhswl 259 47 74 74 CD cord-312431-de7zhswl 259 48 ] ] -RRB- cord-312431-de7zhswl 259 49 . . . cord-312431-de7zhswl 260 1 In in IN cord-312431-de7zhswl 260 2 the the DT cord-312431-de7zhswl 260 3 present present JJ cord-312431-de7zhswl 260 4 study study NN cord-312431-de7zhswl 260 5 six six CD cord-312431-de7zhswl 260 6 cell cell NN cord-312431-de7zhswl 260 7 lines line NNS cord-312431-de7zhswl 260 8 ( ( -LRB- cord-312431-de7zhswl 260 9 the the DT cord-312431-de7zhswl 260 10 A549 A549 NNP cord-312431-de7zhswl 260 11 , , , cord-312431-de7zhswl 260 12 HEK293 HEK293 NNP cord-312431-de7zhswl 260 13 , , , cord-312431-de7zhswl 260 14 Hela Hela NNP cord-312431-de7zhswl 260 15 , , , cord-312431-de7zhswl 260 16 HepG2 HepG2 NNP cord-312431-de7zhswl 260 17 , , , cord-312431-de7zhswl 260 18 PLC PLC NNP cord-312431-de7zhswl 260 19 / / SYM cord-312431-de7zhswl 260 20 PRF/5 PRF/5 NNP cord-312431-de7zhswl 260 21 and and CC cord-312431-de7zhswl 260 22 Vero Vero NNP cord-312431-de7zhswl 260 23 ) ) -RRB- cord-312431-de7zhswl 260 24 were be VBD cord-312431-de7zhswl 260 25 used use VBN cord-312431-de7zhswl 260 26 to to TO cord-312431-de7zhswl 260 27 determine determine VB cord-312431-de7zhswl 260 28 CPE CPE NNP cord-312431-de7zhswl 260 29 of of IN cord-312431-de7zhswl 260 30 the the DT cord-312431-de7zhswl 260 31 VLPs vlp NNS cord-312431-de7zhswl 260 32 from from IN cord-312431-de7zhswl 260 33 the the DT cord-312431-de7zhswl 260 34 Umgeni Umgeni NNP cord-312431-de7zhswl 260 35 River River NNP cord-312431-de7zhswl 260 36 during during IN cord-312431-de7zhswl 260 37 four four CD cord-312431-de7zhswl 260 38 seasons season NNS cord-312431-de7zhswl 260 39 . . . cord-312431-de7zhswl 261 1 All all DT cord-312431-de7zhswl 261 2 VLP VLP NNP cord-312431-de7zhswl 261 3 samples sample NNS cord-312431-de7zhswl 261 4 from from IN cord-312431-de7zhswl 261 5 all all DT cord-312431-de7zhswl 261 6 sites site NNS cord-312431-de7zhswl 261 7 and and CC cord-312431-de7zhswl 261 8 seasons season NNS cord-312431-de7zhswl 261 9 produced produce VBD cord-312431-de7zhswl 261 10 substantial substantial JJ cord-312431-de7zhswl 261 11 CPEs cpe NNS cord-312431-de7zhswl 261 12 on on IN cord-312431-de7zhswl 261 13 the the DT cord-312431-de7zhswl 261 14 Vero Vero NNP cord-312431-de7zhswl 261 15 , , , cord-312431-de7zhswl 261 16 HEK293 HEK293 NNP cord-312431-de7zhswl 261 17 , , , cord-312431-de7zhswl 261 18 Hela Hela NNP cord-312431-de7zhswl 261 19 and and CC cord-312431-de7zhswl 261 20 A549 a549 JJ cord-312431-de7zhswl 261 21 cell cell NN cord-312431-de7zhswl 261 22 lines line NNS cord-312431-de7zhswl 261 23 . . . cord-312431-de7zhswl 262 1 This this DT cord-312431-de7zhswl 262 2 effect effect NN cord-312431-de7zhswl 262 3 was be VBD cord-312431-de7zhswl 262 4 tested test VBN cord-312431-de7zhswl 262 5 against against IN cord-312431-de7zhswl 262 6 positive positive JJ cord-312431-de7zhswl 262 7 controls control NNS cord-312431-de7zhswl 262 8 of of IN cord-312431-de7zhswl 262 9 three three CD cord-312431-de7zhswl 262 10 known know VBN cord-312431-de7zhswl 262 11 viruses virus NNS cord-312431-de7zhswl 262 12 ( ( -LRB- cord-312431-de7zhswl 262 13 voxsackievirus voxsackievirus NNP cord-312431-de7zhswl 262 14 , , , cord-312431-de7zhswl 262 15 rotaviruses rotaviruses NNP cord-312431-de7zhswl 262 16 and and CC cord-312431-de7zhswl 262 17 adenovirus adenovirus NN cord-312431-de7zhswl 262 18 ) ) -RRB- cord-312431-de7zhswl 262 19 in in IN cord-312431-de7zhswl 262 20 the the DT cord-312431-de7zhswl 262 21 same same JJ cord-312431-de7zhswl 262 22 cell cell NN cord-312431-de7zhswl 262 23 lines line NNS cord-312431-de7zhswl 262 24 . . . cord-312431-de7zhswl 263 1 Vero Vero NNP cord-312431-de7zhswl 263 2 cells cell NNS cord-312431-de7zhswl 263 3 are be VBP cord-312431-de7zhswl 263 4 sensitive sensitive JJ cord-312431-de7zhswl 263 5 to to IN cord-312431-de7zhswl 263 6 infection infection NN cord-312431-de7zhswl 263 7 with with IN cord-312431-de7zhswl 263 8 many many JJ cord-312431-de7zhswl 263 9 different different JJ cord-312431-de7zhswl 263 10 viruses virus NNS cord-312431-de7zhswl 263 11 [ [ -LRB- cord-312431-de7zhswl 263 12 74 74 CD cord-312431-de7zhswl 263 13 ] ] -RRB- cord-312431-de7zhswl 263 14 . . . cord-312431-de7zhswl 264 1 Human human JJ cord-312431-de7zhswl 264 2 embryonic embryonic JJ cord-312431-de7zhswl 264 3 kidney kidney NN cord-312431-de7zhswl 264 4 cell cell NN cord-312431-de7zhswl 264 5 line line NN cord-312431-de7zhswl 264 6 293A 293a CD cord-312431-de7zhswl 264 7 and and CC cord-312431-de7zhswl 264 8 human human JJ cord-312431-de7zhswl 264 9 lung lung NN cord-312431-de7zhswl 264 10 carcinoma carcinoma NN cord-312431-de7zhswl 264 11 cell cell NN cord-312431-de7zhswl 264 12 line line NN cord-312431-de7zhswl 264 13 A549 A549 '' cord-312431-de7zhswl 264 14 were be VBD cord-312431-de7zhswl 264 15 the the DT cord-312431-de7zhswl 264 16 most most RBS cord-312431-de7zhswl 264 17 penetrating penetrating JJ cord-312431-de7zhswl 264 18 to to IN cord-312431-de7zhswl 264 19 viral viral JJ cord-312431-de7zhswl 264 20 infection infection NN cord-312431-de7zhswl 264 21 , , , cord-312431-de7zhswl 264 22 especially especially RB cord-312431-de7zhswl 264 23 to to IN cord-312431-de7zhswl 264 24 enteric enteric JJ cord-312431-de7zhswl 264 25 adenovirus adenovirus NN cord-312431-de7zhswl 264 26 40 40 CD cord-312431-de7zhswl 264 27 and and CC cord-312431-de7zhswl 264 28 41 41 CD cord-312431-de7zhswl 264 29 [ [ -LRB- cord-312431-de7zhswl 264 30 75 75 CD cord-312431-de7zhswl 264 31 ] ] -RRB- cord-312431-de7zhswl 264 32 . . . cord-312431-de7zhswl 265 1 Grabow Grabow NNP cord-312431-de7zhswl 265 2 et et NNP cord-312431-de7zhswl 265 3 al al NNP cord-312431-de7zhswl 265 4 . . . cord-312431-de7zhswl 266 1 [ [ -LRB- cord-312431-de7zhswl 266 2 76 76 CD cord-312431-de7zhswl 266 3 ] ] -RRB- cord-312431-de7zhswl 266 4 reported report VBD cord-312431-de7zhswl 266 5 the the DT cord-312431-de7zhswl 266 6 propagation propagation NN cord-312431-de7zhswl 266 7 of of IN cord-312431-de7zhswl 266 8 both both DT cord-312431-de7zhswl 266 9 enteric enteric JJ cord-312431-de7zhswl 266 10 adenoviruses adenovirus NNS cord-312431-de7zhswl 266 11 40 40 CD cord-312431-de7zhswl 266 12 and and CC cord-312431-de7zhswl 266 13 41 41 CD cord-312431-de7zhswl 266 14 in in IN cord-312431-de7zhswl 266 15 the the DT cord-312431-de7zhswl 266 16 primary primary JJ cord-312431-de7zhswl 266 17 liver liver NN cord-312431-de7zhswl 266 18 carcinoma carcinoma NN cord-312431-de7zhswl 266 19 ( ( -LRB- cord-312431-de7zhswl 266 20 PLC PLC NNP cord-312431-de7zhswl 266 21 / / SYM cord-312431-de7zhswl 266 22 PRF/5 PRF/5 NNP cord-312431-de7zhswl 266 23 ) ) -RRB- cord-312431-de7zhswl 266 24 cell cell NN cord-312431-de7zhswl 266 25 line line NN cord-312431-de7zhswl 266 26 . . . cord-312431-de7zhswl 267 1 Hep Hep NNP cord-312431-de7zhswl 267 2 - - HYPH cord-312431-de7zhswl 267 3 G2 G2 NNP cord-312431-de7zhswl 267 4 cell cell NN cord-312431-de7zhswl 267 5 lines line NNS cord-312431-de7zhswl 267 6 are be VBP cord-312431-de7zhswl 267 7 often often RB cord-312431-de7zhswl 267 8 used use VBN cord-312431-de7zhswl 267 9 to to TO cord-312431-de7zhswl 267 10 study study VB cord-312431-de7zhswl 267 11 Hepatitis Hepatitis NNP cord-312431-de7zhswl 267 12 B B NNP cord-312431-de7zhswl 267 13 , , , cord-312431-de7zhswl 267 14 C C NNP cord-312431-de7zhswl 267 15 and and CC cord-312431-de7zhswl 267 16 other other JJ cord-312431-de7zhswl 267 17 viruses virus NNS cord-312431-de7zhswl 267 18 [ [ -LRB- cord-312431-de7zhswl 267 19 74 74 CD cord-312431-de7zhswl 267 20 ] ] -RRB- cord-312431-de7zhswl 267 21 . . . cord-312431-de7zhswl 268 1 CPE CPE NNP cord-312431-de7zhswl 268 2 of of IN cord-312431-de7zhswl 268 3 the the DT cord-312431-de7zhswl 268 4 VLPs vlp NNS cord-312431-de7zhswl 268 5 was be VBD cord-312431-de7zhswl 268 6 examined examine VBN cord-312431-de7zhswl 268 7 by by IN cord-312431-de7zhswl 268 8 microscopy microscopy NNS cord-312431-de7zhswl 268 9 every every DT cord-312431-de7zhswl 268 10 day day NN cord-312431-de7zhswl 268 11 for for IN cord-312431-de7zhswl 268 12 the the DT cord-312431-de7zhswl 268 13 first first JJ cord-312431-de7zhswl 268 14 week week NN cord-312431-de7zhswl 268 15 of of IN cord-312431-de7zhswl 268 16 growth growth NN cord-312431-de7zhswl 268 17 to to TO cord-312431-de7zhswl 268 18 maximise maximise VB cord-312431-de7zhswl 268 19 the the DT cord-312431-de7zhswl 268 20 detection detection NN cord-312431-de7zhswl 268 21 of of IN cord-312431-de7zhswl 268 22 viral viral JJ cord-312431-de7zhswl 268 23 progression progression NN cord-312431-de7zhswl 268 24 . . . cord-312431-de7zhswl 269 1 Certain certain JJ cord-312431-de7zhswl 269 2 viruses virus NNS cord-312431-de7zhswl 269 3 take take VBP cord-312431-de7zhswl 269 4 one one CD cord-312431-de7zhswl 269 5 day day NN cord-312431-de7zhswl 269 6 to to IN cord-312431-de7zhswl 269 7 three three CD cord-312431-de7zhswl 269 8 weeks week NNS cord-312431-de7zhswl 269 9 to to TO cord-312431-de7zhswl 269 10 yield yield VB cord-312431-de7zhswl 269 11 a a DT cord-312431-de7zhswl 269 12 CPE CPE NNP cord-312431-de7zhswl 269 13 dependent dependent JJ cord-312431-de7zhswl 269 14 on on IN cord-312431-de7zhswl 269 15 the the DT cord-312431-de7zhswl 269 16 initial initial JJ cord-312431-de7zhswl 269 17 concentration concentration NN cord-312431-de7zhswl 269 18 and and CC cord-312431-de7zhswl 269 19 virus virus NN cord-312431-de7zhswl 269 20 type type NN cord-312431-de7zhswl 269 21 . . . cord-312431-de7zhswl 270 1 CPE CPE NNP cord-312431-de7zhswl 270 2 can can MD cord-312431-de7zhswl 270 3 be be VB cord-312431-de7zhswl 270 4 swelling swell VBG cord-312431-de7zhswl 270 5 , , , cord-312431-de7zhswl 270 6 shrinking shrink VBG cord-312431-de7zhswl 270 7 , , , cord-312431-de7zhswl 270 8 rounding rounding NN cord-312431-de7zhswl 270 9 of of IN cord-312431-de7zhswl 270 10 cells cell NNS cord-312431-de7zhswl 270 11 to to IN cord-312431-de7zhswl 270 12 grouping group VBG cord-312431-de7zhswl 270 13 or or CC cord-312431-de7zhswl 270 14 whole whole JJ cord-312431-de7zhswl 270 15 destruction destruction NN cord-312431-de7zhswl 270 16 of of IN cord-312431-de7zhswl 270 17 the the DT cord-312431-de7zhswl 270 18 monolayer monolayer NN cord-312431-de7zhswl 270 19 [ [ -LRB- cord-312431-de7zhswl 270 20 77 77 CD cord-312431-de7zhswl 270 21 ] ] -RRB- cord-312431-de7zhswl 270 22 . . . cord-312431-de7zhswl 271 1 All all PDT cord-312431-de7zhswl 271 2 the the DT cord-312431-de7zhswl 271 3 VLPs vlp NNS cord-312431-de7zhswl 271 4 isolated isolate VBN cord-312431-de7zhswl 271 5 from from IN cord-312431-de7zhswl 271 6 water water NN cord-312431-de7zhswl 271 7 samples sample NNS cord-312431-de7zhswl 271 8 were be VBD cord-312431-de7zhswl 271 9 capable capable JJ cord-312431-de7zhswl 271 10 of of IN cord-312431-de7zhswl 271 11 inducing induce VBG cord-312431-de7zhswl 271 12 the the DT cord-312431-de7zhswl 271 13 CPEs cpe NNS cord-312431-de7zhswl 271 14 on on IN cord-312431-de7zhswl 271 15 six six CD cord-312431-de7zhswl 271 16 tissue tissue NN cord-312431-de7zhswl 271 17 cell cell NN cord-312431-de7zhswl 271 18 lines line NNS cord-312431-de7zhswl 271 19 . . . cord-312431-de7zhswl 272 1 The the DT cord-312431-de7zhswl 272 2 ability ability NN cord-312431-de7zhswl 272 3 of of IN cord-312431-de7zhswl 272 4 these these DT cord-312431-de7zhswl 272 5 viruses virus NNS cord-312431-de7zhswl 272 6 to to TO cord-312431-de7zhswl 272 7 infect infect VB cord-312431-de7zhswl 272 8 susceptible susceptible JJ cord-312431-de7zhswl 272 9 host host NN cord-312431-de7zhswl 272 10 cells cell NNS cord-312431-de7zhswl 272 11 and and CC cord-312431-de7zhswl 272 12 to to TO cord-312431-de7zhswl 272 13 replicate replicate VB cord-312431-de7zhswl 272 14 their -PRON- PRP$ cord-312431-de7zhswl 272 15 DNA DNA NNP cord-312431-de7zhswl 272 16 / / SYM cord-312431-de7zhswl 272 17 RNA RNA NNP cord-312431-de7zhswl 272 18 , , , cord-312431-de7zhswl 272 19 confirms confirm VBZ cord-312431-de7zhswl 272 20 that that IN cord-312431-de7zhswl 272 21 they -PRON- PRP cord-312431-de7zhswl 272 22 are be VBP cord-312431-de7zhswl 272 23 viable viable JJ cord-312431-de7zhswl 272 24 and and CC cord-312431-de7zhswl 272 25 infectious infectious JJ cord-312431-de7zhswl 272 26 and and CC cord-312431-de7zhswl 272 27 therefore therefore RB cord-312431-de7zhswl 272 28 constitute constitute VBP cord-312431-de7zhswl 272 29 a a DT cord-312431-de7zhswl 272 30 health health NN cord-312431-de7zhswl 272 31 risk risk NN cord-312431-de7zhswl 272 32 which which WDT cord-312431-de7zhswl 272 33 is be VBZ cord-312431-de7zhswl 272 34 due due JJ cord-312431-de7zhswl 272 35 to to TO cord-312431-de7zhswl 272 36 be be VB cord-312431-de7zhswl 272 37 investigated investigate VBN cord-312431-de7zhswl 272 38 [ [ -LRB- cord-312431-de7zhswl 272 39 18 18 CD cord-312431-de7zhswl 272 40 , , , cord-312431-de7zhswl 272 41 78 78 CD cord-312431-de7zhswl 272 42 ] ] -RRB- cord-312431-de7zhswl 272 43 . . . cord-312431-de7zhswl 273 1 In in IN cord-312431-de7zhswl 273 2 this this DT cord-312431-de7zhswl 273 3 study study NN cord-312431-de7zhswl 273 4 , , , cord-312431-de7zhswl 273 5 different different JJ cord-312431-de7zhswl 273 6 approaches approach NNS cord-312431-de7zhswl 273 7 to to TO cord-312431-de7zhswl 273 8 determine determine VB cord-312431-de7zhswl 273 9 viral viral JJ cord-312431-de7zhswl 273 10 pollution pollution NN cord-312431-de7zhswl 273 11 during during IN cord-312431-de7zhswl 273 12 four four CD cord-312431-de7zhswl 273 13 seasons season NNS cord-312431-de7zhswl 273 14 and and CC cord-312431-de7zhswl 273 15 at at IN cord-312431-de7zhswl 273 16 five five CD cord-312431-de7zhswl 273 17 sampling sampling NN cord-312431-de7zhswl 273 18 points point NNS cord-312431-de7zhswl 273 19 in in IN cord-312431-de7zhswl 273 20 the the DT cord-312431-de7zhswl 273 21 Umgeni Umgeni NNP cord-312431-de7zhswl 273 22 River River NNP cord-312431-de7zhswl 273 23 were be VBD cord-312431-de7zhswl 273 24 comparatively comparatively RB cord-312431-de7zhswl 273 25 evaluated evaluate VBN cord-312431-de7zhswl 273 26 . . . cord-312431-de7zhswl 274 1 A a DT cord-312431-de7zhswl 274 2 setup setup NN cord-312431-de7zhswl 274 3 for for IN cord-312431-de7zhswl 274 4 the the DT cord-312431-de7zhswl 274 5 concentration concentration NN cord-312431-de7zhswl 274 6 of of IN cord-312431-de7zhswl 274 7 viruses virus NNS cord-312431-de7zhswl 274 8 from from IN cord-312431-de7zhswl 274 9 large large JJ cord-312431-de7zhswl 274 10 ( ( -LRB- cord-312431-de7zhswl 274 11 20 20 CD cord-312431-de7zhswl 274 12 L l NN cord-312431-de7zhswl 274 13 ) ) -RRB- cord-312431-de7zhswl 274 14 volume volume NN cord-312431-de7zhswl 274 15 water water NN cord-312431-de7zhswl 274 16 samples sample NNS cord-312431-de7zhswl 274 17 using use VBG cord-312431-de7zhswl 274 18 a a DT cord-312431-de7zhswl 274 19 two two CD cord-312431-de7zhswl 274 20 - - HYPH cord-312431-de7zhswl 274 21 step step NN cord-312431-de7zhswl 274 22 TFF tff NN cord-312431-de7zhswl 274 23 process process NN cord-312431-de7zhswl 274 24 was be VBD cord-312431-de7zhswl 274 25 successfully successfully RB cord-312431-de7zhswl 274 26 established establish VBN cord-312431-de7zhswl 274 27 . . . cord-312431-de7zhswl 275 1 Electron electron NN cord-312431-de7zhswl 275 2 microscopy microscopy NN cord-312431-de7zhswl 275 3 illustrated illustrate VBD cord-312431-de7zhswl 275 4 the the DT cord-312431-de7zhswl 275 5 presence presence NN cord-312431-de7zhswl 275 6 of of IN cord-312431-de7zhswl 275 7 VLPs vlp NNS cord-312431-de7zhswl 275 8 in in IN cord-312431-de7zhswl 275 9 concentrated concentrated JJ cord-312431-de7zhswl 275 10 water water NN cord-312431-de7zhswl 275 11 samples sample NNS cord-312431-de7zhswl 275 12 at at IN cord-312431-de7zhswl 275 13 all all DT cord-312431-de7zhswl 275 14 sites site NNS cord-312431-de7zhswl 275 15 sampled sample VBN cord-312431-de7zhswl 275 16 , , , cord-312431-de7zhswl 275 17 and and CC cord-312431-de7zhswl 275 18 their -PRON- PRP$ cord-312431-de7zhswl 275 19 infectivity infectivity NN cord-312431-de7zhswl 275 20 was be VBD cord-312431-de7zhswl 275 21 established establish VBN cord-312431-de7zhswl 275 22 based base VBN cord-312431-de7zhswl 275 23 on on IN cord-312431-de7zhswl 275 24 their -PRON- PRP$ cord-312431-de7zhswl 275 25 CPE CPE NNP cord-312431-de7zhswl 275 26 on on IN cord-312431-de7zhswl 275 27 various various JJ cord-312431-de7zhswl 275 28 cell cell NN cord-312431-de7zhswl 275 29 lines line NNS cord-312431-de7zhswl 275 30 . . . cord-312431-de7zhswl 276 1 The the DT cord-312431-de7zhswl 276 2 results result NNS cord-312431-de7zhswl 276 3 produced produce VBN cord-312431-de7zhswl 276 4 suggest suggest VBP cord-312431-de7zhswl 276 5 the the DT cord-312431-de7zhswl 276 6 infectious infectious JJ cord-312431-de7zhswl 276 7 potentials potential NNS cord-312431-de7zhswl 276 8 of of IN cord-312431-de7zhswl 276 9 the the DT cord-312431-de7zhswl 276 10 VLPs vlp NNS cord-312431-de7zhswl 276 11 in in IN cord-312431-de7zhswl 276 12 the the DT cord-312431-de7zhswl 276 13 Umgeni Umgeni NNP cord-312431-de7zhswl 276 14 River River NNP cord-312431-de7zhswl 276 15 water water NN cord-312431-de7zhswl 276 16 samples sample NNS cord-312431-de7zhswl 276 17 , , , cord-312431-de7zhswl 276 18 especially especially RB cord-312431-de7zhswl 276 19 river river NN cord-312431-de7zhswl 276 20 water water NN cord-312431-de7zhswl 276 21 from from IN cord-312431-de7zhswl 276 22 the the DT cord-312431-de7zhswl 276 23 lower low JJR cord-312431-de7zhswl 276 24 catchment catchment NN cord-312431-de7zhswl 276 25 areas area NNS cord-312431-de7zhswl 276 26 , , , cord-312431-de7zhswl 276 27 to to TO cord-312431-de7zhswl 276 28 infect infect VB cord-312431-de7zhswl 276 29 the the DT cord-312431-de7zhswl 276 30 human human JJ cord-312431-de7zhswl 276 31 hosts host NNS cord-312431-de7zhswl 276 32 throughout throughout IN cord-312431-de7zhswl 276 33 the the DT cord-312431-de7zhswl 276 34 year year NN cord-312431-de7zhswl 276 35 . . . cord-312431-de7zhswl 277 1 These these DT cord-312431-de7zhswl 277 2 observations observation NNS cord-312431-de7zhswl 277 3 have have VBP cord-312431-de7zhswl 277 4 serious serious JJ cord-312431-de7zhswl 277 5 health health NN cord-312431-de7zhswl 277 6 care care NN cord-312431-de7zhswl 277 7 implications implication NNS cord-312431-de7zhswl 277 8 if if IN cord-312431-de7zhswl 277 9 raw raw JJ cord-312431-de7zhswl 277 10 untreated untreated JJ cord-312431-de7zhswl 277 11 water water NN cord-312431-de7zhswl 277 12 is be VBZ cord-312431-de7zhswl 277 13 used use VBN cord-312431-de7zhswl 277 14 for for IN cord-312431-de7zhswl 277 15 human human JJ cord-312431-de7zhswl 277 16 consumption consumption NN cord-312431-de7zhswl 277 17 . . . cord-312431-de7zhswl 278 1 However however RB cord-312431-de7zhswl 278 2 , , , cord-312431-de7zhswl 278 3 it -PRON- PRP cord-312431-de7zhswl 278 4 should should MD cord-312431-de7zhswl 278 5 also also RB cord-312431-de7zhswl 278 6 be be VB cord-312431-de7zhswl 278 7 considered consider VBN cord-312431-de7zhswl 278 8 that that IN cord-312431-de7zhswl 278 9 not not RB cord-312431-de7zhswl 278 10 all all DT cord-312431-de7zhswl 278 11 viral viral JJ cord-312431-de7zhswl 278 12 particles particle NNS cord-312431-de7zhswl 278 13 detected detect VBN cord-312431-de7zhswl 278 14 in in IN cord-312431-de7zhswl 278 15 this this DT cord-312431-de7zhswl 278 16 study study NN cord-312431-de7zhswl 278 17 correspond correspond VB cord-312431-de7zhswl 278 18 to to IN cord-312431-de7zhswl 278 19 infectious infectious JJ cord-312431-de7zhswl 278 20 viral viral JJ cord-312431-de7zhswl 278 21 particles particle NNS cord-312431-de7zhswl 278 22 and and CC cord-312431-de7zhswl 278 23 a a DT cord-312431-de7zhswl 278 24 high high JJ cord-312431-de7zhswl 278 25 percentage percentage NN cord-312431-de7zhswl 278 26 of of IN cord-312431-de7zhswl 278 27 non non JJ cord-312431-de7zhswl 278 28 - - JJ cord-312431-de7zhswl 278 29 infectious infectious JJ cord-312431-de7zhswl 278 30 viral viral JJ cord-312431-de7zhswl 278 31 particles particle NNS cord-312431-de7zhswl 278 32 may may MD cord-312431-de7zhswl 278 33 be be VB cord-312431-de7zhswl 278 34 present present JJ cord-312431-de7zhswl 278 35 in in IN cord-312431-de7zhswl 278 36 the the DT cord-312431-de7zhswl 278 37 environment environment NN cord-312431-de7zhswl 278 38 [ [ -LRB- cord-312431-de7zhswl 278 39 76 76 CD cord-312431-de7zhswl 278 40 ] ] -RRB- cord-312431-de7zhswl 278 41 . . . cord-312431-de7zhswl 279 1 The the DT cord-312431-de7zhswl 279 2 present present JJ cord-312431-de7zhswl 279 3 study study NN cord-312431-de7zhswl 279 4 thus thus RB cord-312431-de7zhswl 279 5 highlights highlight VBZ cord-312431-de7zhswl 279 6 the the DT cord-312431-de7zhswl 279 7 importance importance NN cord-312431-de7zhswl 279 8 of of IN cord-312431-de7zhswl 279 9 routine routine JJ cord-312431-de7zhswl 279 10 environmental environmental JJ cord-312431-de7zhswl 279 11 surveillance surveillance NN cord-312431-de7zhswl 279 12 of of IN cord-312431-de7zhswl 279 13 human human JJ cord-312431-de7zhswl 279 14 enteric enteric JJ cord-312431-de7zhswl 279 15 viruses virus NNS cord-312431-de7zhswl 279 16 for for IN cord-312431-de7zhswl 279 17 a a DT cord-312431-de7zhswl 279 18 better well JJR cord-312431-de7zhswl 279 19 understanding understanding NN cord-312431-de7zhswl 279 20 of of IN cord-312431-de7zhswl 279 21 the the DT cord-312431-de7zhswl 279 22 actual actual JJ cord-312431-de7zhswl 279 23 burden burden NN cord-312431-de7zhswl 279 24 of of IN cord-312431-de7zhswl 279 25 these these DT cord-312431-de7zhswl 279 26 viral viral JJ cord-312431-de7zhswl 279 27 infections infection NNS cord-312431-de7zhswl 279 28 on on IN cord-312431-de7zhswl 279 29 those those DT cord-312431-de7zhswl 279 30 who who WP cord-312431-de7zhswl 279 31 might may MD cord-312431-de7zhswl 279 32 be be VB cord-312431-de7zhswl 279 33 using use VBG cord-312431-de7zhswl 279 34 the the DT cord-312431-de7zhswl 279 35 water water NN cord-312431-de7zhswl 279 36 directly directly RB cord-312431-de7zhswl 279 37 without without IN cord-312431-de7zhswl 279 38 treatment treatment NN cord-312431-de7zhswl 279 39 . . . cord-312431-de7zhswl 280 1 Methods method NNS cord-312431-de7zhswl 280 2 to to TO cord-312431-de7zhswl 280 3 Detect detect VB cord-312431-de7zhswl 280 4 Infectious infectious JJ cord-312431-de7zhswl 280 5 Human human JJ cord-312431-de7zhswl 280 6 Enteric Enteric NNP cord-312431-de7zhswl 280 7 Viruses Viruses NNPS cord-312431-de7zhswl 280 8 in in IN cord-312431-de7zhswl 280 9 Environmental Environmental NNP cord-312431-de7zhswl 280 10 Water Water NNP cord-312431-de7zhswl 280 11 Samples Samples NNPS cord-312431-de7zhswl 280 12 Application Application NNP cord-312431-de7zhswl 280 13 of of IN cord-312431-de7zhswl 280 14 Enteric enteric JJ cord-312431-de7zhswl 280 15 Viruses virus NNS cord-312431-de7zhswl 281 1 Faecal Faecal NNP cord-312431-de7zhswl 281 2 Pollution Pollution NNP cord-312431-de7zhswl 281 3 Source Source NNP cord-312431-de7zhswl 281 4 Tracking tracking NN cord-312431-de7zhswl 281 5 in in IN cord-312431-de7zhswl 281 6 Environmental Environmental NNP cord-312431-de7zhswl 281 7 Waters Waters NNPS cord-312431-de7zhswl 281 8 Health Health NNP cord-312431-de7zhswl 281 9 Aspects Aspects NNPS cord-312431-de7zhswl 281 10 of of IN cord-312431-de7zhswl 281 11 Virological Virological NNP cord-312431-de7zhswl 281 12 Water Water NNP cord-312431-de7zhswl 281 13 Quality Quality NNP cord-312431-de7zhswl 281 14 : : : cord-312431-de7zhswl 282 1 An an DT cord-312431-de7zhswl 282 2 Overview Overview NNP cord-312431-de7zhswl 282 3 Review Review NNP cord-312431-de7zhswl 282 4 Guidelines Guidelines NNPS cord-312431-de7zhswl 282 5 for for IN cord-312431-de7zhswl 282 6 Drinking Drinking NNP cord-312431-de7zhswl 282 7 Water Water NNP cord-312431-de7zhswl 282 8 Quality Quality NNP cord-312431-de7zhswl 282 9 , , , cord-312431-de7zhswl 282 10 Recommendations Recommendations NNPS cord-312431-de7zhswl 282 11 Alternative Alternative NNP cord-312431-de7zhswl 282 12 Indicators Indicators NNPS cord-312431-de7zhswl 282 13 of of IN cord-312431-de7zhswl 282 14 Faecal Faecal NNP cord-312431-de7zhswl 282 15 Pollution Pollution NNP cord-312431-de7zhswl 282 16 : : : cord-312431-de7zhswl 283 1 Relations relation NNS cord-312431-de7zhswl 283 2 with with IN cord-312431-de7zhswl 283 3 Pathogens Pathogens NNPS cord-312431-de7zhswl 283 4 and and CC cord-312431-de7zhswl 283 5 Conventional Conventional NNP cord-312431-de7zhswl 283 6 Indicators Indicators NNPS cord-312431-de7zhswl 283 7 , , , cord-312431-de7zhswl 283 8 Current Current NNP cord-312431-de7zhswl 283 9 Methodologies Methodologies NNPS cord-312431-de7zhswl 283 10 for for IN cord-312431-de7zhswl 283 11 Direct Direct NNP cord-312431-de7zhswl 283 12 Pathogen Pathogen NNP cord-312431-de7zhswl 283 13 Monitoring Monitoring NNP cord-312431-de7zhswl 283 14 and and CC cord-312431-de7zhswl 283 15 Future Future NNP cord-312431-de7zhswl 283 16 Application Application NNP cord-312431-de7zhswl 283 17 Perspectives Perspectives NNPS cord-312431-de7zhswl 283 18 Role role NN cord-312431-de7zhswl 283 19 of of IN cord-312431-de7zhswl 283 20 Non non JJ cord-312431-de7zhswl 283 21 - - JJ cord-312431-de7zhswl 283 22 host host JJ cord-312431-de7zhswl 283 23 Environment Environment NNP cord-312431-de7zhswl 283 24 in in IN cord-312431-de7zhswl 283 25 the the DT cord-312431-de7zhswl 283 26 Lifestyle Lifestyle NNP cord-312431-de7zhswl 283 27 of of IN cord-312431-de7zhswl 283 28 Salmonella Salmonella NNP cord-312431-de7zhswl 283 29 and and CC cord-312431-de7zhswl 283 30 E. E. NNP cord-312431-de7zhswl 283 31 coli coli NNS cord-312431-de7zhswl 284 1 Campylobacter Campylobacter NNP cord-312431-de7zhswl 284 2 spp spp NNS cord-312431-de7zhswl 284 3 . . . cord-312431-de7zhswl 285 1 , , , cord-312431-de7zhswl 285 2 Giardia Giardia NNP cord-312431-de7zhswl 285 3 spp spp NNS cord-312431-de7zhswl 285 4 . . . cord-312431-de7zhswl 286 1 , , , cord-312431-de7zhswl 286 2 Cryptosporidium cryptosporidium NN cord-312431-de7zhswl 286 3 spp spp NNS cord-312431-de7zhswl 286 4 . . . cord-312431-de7zhswl 287 1 , , , cord-312431-de7zhswl 287 2 Noroviruses Noroviruses NNP cord-312431-de7zhswl 287 3 , , , cord-312431-de7zhswl 287 4 and and CC cord-312431-de7zhswl 287 5 Indicator Indicator NNP cord-312431-de7zhswl 287 6 Organisms Organisms NNPS cord-312431-de7zhswl 287 7 in in IN cord-312431-de7zhswl 287 8 Surface Surface NNP cord-312431-de7zhswl 287 9 Water Water NNP cord-312431-de7zhswl 287 10 in in IN cord-312431-de7zhswl 287 11 South South NNP cord-312431-de7zhswl 287 12 Western Western NNP cord-312431-de7zhswl 287 13 Finland Finland NNP cord-312431-de7zhswl 287 14 Water Water NNP cord-312431-de7zhswl 287 15 - - HYPH cord-312431-de7zhswl 287 16 Related Related NNP cord-312431-de7zhswl 287 17 Disease Disease NNP cord-312431-de7zhswl 287 18 Outbreaks Outbreaks NNPS cord-312431-de7zhswl 287 19 Seasonal Seasonal NNP cord-312431-de7zhswl 287 20 Detection Detection NNP cord-312431-de7zhswl 287 21 of of IN cord-312431-de7zhswl 287 22 Human Human NNP cord-312431-de7zhswl 287 23 Viruses Viruses NNPS cord-312431-de7zhswl 287 24 and and CC cord-312431-de7zhswl 287 25 Coliphage Coliphage NNP cord-312431-de7zhswl 287 26 in in IN cord-312431-de7zhswl 287 27 Detection detection NN cord-312431-de7zhswl 287 28 of of IN cord-312431-de7zhswl 287 29 Naturally naturally RB cord-312431-de7zhswl 287 30 Occurring occur VBG cord-312431-de7zhswl 287 31 Enteroviruses Enteroviruses NNP cord-312431-de7zhswl 287 32 in in IN cord-312431-de7zhswl 287 33 Waters Waters NNPS cord-312431-de7zhswl 287 34 by by IN cord-312431-de7zhswl 287 35 Reverse Reverse NNP cord-312431-de7zhswl 287 36 Transcription Transcription NNP cord-312431-de7zhswl 287 37 - - HYPH cord-312431-de7zhswl 287 38 Polymerase Polymerase NNP cord-312431-de7zhswl 287 39 Chain Chain NNP cord-312431-de7zhswl 287 40 Reaction reaction NN cord-312431-de7zhswl 287 41 and and CC cord-312431-de7zhswl 287 42 Hybridization hybridization NN cord-312431-de7zhswl 287 43 Estimates estimate NNS cord-312431-de7zhswl 287 44 of of IN cord-312431-de7zhswl 287 45 the the DT cord-312431-de7zhswl 287 46 Severity severity NN cord-312431-de7zhswl 287 47 of of IN cord-312431-de7zhswl 287 48 Illnesses illness NNS cord-312431-de7zhswl 287 49 Associated associate VBN cord-312431-de7zhswl 287 50 with with IN cord-312431-de7zhswl 287 51 Bathing Bathing NNP cord-312431-de7zhswl 287 52 in in IN cord-312431-de7zhswl 287 53 Marine Marine NNP cord-312431-de7zhswl 287 54 Recreational Recreational NNP cord-312431-de7zhswl 287 55 Waters Waters NNPS cord-312431-de7zhswl 287 56 Contaminated contaminate VBN cord-312431-de7zhswl 287 57 with with IN cord-312431-de7zhswl 287 58 Domestic Domestic NNP cord-312431-de7zhswl 287 59 Sewage Sewage NNP cord-312431-de7zhswl 287 60 Environmental Environmental NNP cord-312431-de7zhswl 287 61 Virology Virology NNP cord-312431-de7zhswl 287 62 : : : cord-312431-de7zhswl 288 1 From from IN cord-312431-de7zhswl 288 2 Detection detection NN cord-312431-de7zhswl 288 3 of of IN cord-312431-de7zhswl 288 4 Virus Virus NNP cord-312431-de7zhswl 288 5 in in IN cord-312431-de7zhswl 288 6 Sewage Sewage NNP cord-312431-de7zhswl 288 7 and and CC cord-312431-de7zhswl 288 8 Water Water NNP cord-312431-de7zhswl 288 9 by by IN cord-312431-de7zhswl 288 10 Isolation Isolation NNP cord-312431-de7zhswl 288 11 to to IN cord-312431-de7zhswl 288 12 Identification identification NN cord-312431-de7zhswl 288 13 by by IN cord-312431-de7zhswl 288 14 Molecular Molecular NNP cord-312431-de7zhswl 288 15 Biology Biology NNP cord-312431-de7zhswl 288 16 : : : cord-312431-de7zhswl 289 1 A a DT cord-312431-de7zhswl 289 2 Trip trip NN cord-312431-de7zhswl 289 3 of of IN cord-312431-de7zhswl 289 4 Over over IN cord-312431-de7zhswl 289 5 50 50 CD cord-312431-de7zhswl 289 6 Years year NNS cord-312431-de7zhswl 289 7 Distribution distribution NN cord-312431-de7zhswl 289 8 of of IN cord-312431-de7zhswl 289 9 viruses virus NNS cord-312431-de7zhswl 289 10 in in IN cord-312431-de7zhswl 289 11 the the DT cord-312431-de7zhswl 289 12 water water NN cord-312431-de7zhswl 289 13 environment environment NN cord-312431-de7zhswl 289 14 Detection detection NN cord-312431-de7zhswl 289 15 of of IN cord-312431-de7zhswl 289 16 Infectious infectious JJ cord-312431-de7zhswl 289 17 Adenoviruses Adenoviruses NNPS cord-312431-de7zhswl 289 18 in in IN cord-312431-de7zhswl 289 19 Environmental Environmental NNP cord-312431-de7zhswl 289 20 Waters Waters NNPS cord-312431-de7zhswl 289 21 by by IN cord-312431-de7zhswl 289 22 Fluorescence Fluorescence NNP cord-312431-de7zhswl 289 23 - - HYPH cord-312431-de7zhswl 289 24 Activated Activated NNP cord-312431-de7zhswl 289 25 Cell Cell NNP cord-312431-de7zhswl 289 26 Sorting Sorting NNP cord-312431-de7zhswl 289 27 Assay Assay NNP cord-312431-de7zhswl 289 28 Microbial microbial JJ cord-312431-de7zhswl 289 29 Pathogens Pathogens NNPS cord-312431-de7zhswl 289 30 in in IN cord-312431-de7zhswl 289 31 the the DT cord-312431-de7zhswl 289 32 Umgeni Umgeni NNP cord-312431-de7zhswl 289 33 River River NNP cord-312431-de7zhswl 289 34 Occurrence Occurrence NNP cord-312431-de7zhswl 289 35 and and CC cord-312431-de7zhswl 289 36 Levels Levels NNPS cord-312431-de7zhswl 289 37 of of IN cord-312431-de7zhswl 289 38 Indicator Indicator NNP cord-312431-de7zhswl 289 39 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 289 40 in in IN cord-312431-de7zhswl 289 41 Bathing Bathing NNP cord-312431-de7zhswl 289 42 Waters Waters NNPS cord-312431-de7zhswl 289 43 throughout throughout IN cord-312431-de7zhswl 289 44 Europe Europe NNP cord-312431-de7zhswl 289 45 F F NNP cord-312431-de7zhswl 289 46 - - HYPH cord-312431-de7zhswl 289 47 Specific Specific NNP cord-312431-de7zhswl 289 48 RNA RNA NNP cord-312431-de7zhswl 289 49 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 289 50 are be VBP cord-312431-de7zhswl 289 51 Adequate adequate JJ cord-312431-de7zhswl 289 52 Model Model NNP cord-312431-de7zhswl 289 53 Organisms Organisms NNPS cord-312431-de7zhswl 289 54 for for IN cord-312431-de7zhswl 289 55 Enteric enteric JJ cord-312431-de7zhswl 289 56 Viruses Viruses NNPS cord-312431-de7zhswl 289 57 in in IN cord-312431-de7zhswl 289 58 Fresh Fresh NNP cord-312431-de7zhswl 289 59 Water Water NNP cord-312431-de7zhswl 289 60 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 289 61 : : : cord-312431-de7zhswl 290 1 Update update NN cord-312431-de7zhswl 290 2 on on IN cord-312431-de7zhswl 290 3 Application Application NNP cord-312431-de7zhswl 290 4 as as IN cord-312431-de7zhswl 290 5 Models model NNS cord-312431-de7zhswl 290 6 for for IN cord-312431-de7zhswl 290 7 Viruses Viruses NNPS cord-312431-de7zhswl 290 8 in in IN cord-312431-de7zhswl 290 9 Water Water NNP cord-312431-de7zhswl 290 10 Occurrence Occurrence NNP cord-312431-de7zhswl 290 11 of of IN cord-312431-de7zhswl 290 12 Male male NN cord-312431-de7zhswl 290 13 - - HYPH cord-312431-de7zhswl 290 14 Specific specific JJ cord-312431-de7zhswl 290 15 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 290 16 in in IN cord-312431-de7zhswl 290 17 Feral feral JJ cord-312431-de7zhswl 290 18 and and CC cord-312431-de7zhswl 290 19 Domestic Domestic NNP cord-312431-de7zhswl 290 20 Animal Animal NNP cord-312431-de7zhswl 290 21 Wastes Wastes NNPS cord-312431-de7zhswl 290 22 , , , cord-312431-de7zhswl 290 23 Human Human NNP cord-312431-de7zhswl 290 24 Faeces Faeces NNPS cord-312431-de7zhswl 290 25 , , , cord-312431-de7zhswl 290 26 and and CC cord-312431-de7zhswl 290 27 Human Human NNP cord-312431-de7zhswl 290 28 - - HYPH cord-312431-de7zhswl 290 29 Associated Associated NNP cord-312431-de7zhswl 290 30 Wastewaters Wastewaters NNPS cord-312431-de7zhswl 291 1 South south JJ cord-312431-de7zhswl 291 2 African African NNP cord-312431-de7zhswl 291 3 Water Water NNP cord-312431-de7zhswl 291 4 Quality Quality NNP cord-312431-de7zhswl 291 5 Guidelines Guidelines NNPS cord-312431-de7zhswl 292 1 -Domestic -domestic JJ cord-312431-de7zhswl 293 1 Uses use VBZ cord-312431-de7zhswl 293 2 Study Study NNP cord-312431-de7zhswl 293 3 Group Group NNP cord-312431-de7zhswl 293 4 on on IN cord-312431-de7zhswl 293 5 Health Health NNP cord-312431-de7zhswl 293 6 - - HYPH cord-312431-de7zhswl 293 7 Related Related NNP cord-312431-de7zhswl 293 8 Water Water NNP cord-312431-de7zhswl 293 9 Microbiology Microbiology NNP cord-312431-de7zhswl 293 10 , , , cord-312431-de7zhswl 293 11 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 293 12 as as IN cord-312431-de7zhswl 293 13 Model Model NNP cord-312431-de7zhswl 293 14 Viruses Viruses NNPS cord-312431-de7zhswl 293 15 in in IN cord-312431-de7zhswl 293 16 Water Water NNP cord-312431-de7zhswl 293 17 Quality Quality NNP cord-312431-de7zhswl 293 18 Control Control NNP cord-312431-de7zhswl 293 19 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 293 20 as as IN cord-312431-de7zhswl 293 21 Indicators indicator NNS cord-312431-de7zhswl 293 22 of of IN cord-312431-de7zhswl 293 23 Enteric Enteric NNP cord-312431-de7zhswl 293 24 Viruses Viruses NNPS cord-312431-de7zhswl 293 25 and and CC cord-312431-de7zhswl 293 26 Public Public NNP cord-312431-de7zhswl 293 27 Health Health NNP cord-312431-de7zhswl 293 28 Risk Risk NNP cord-312431-de7zhswl 293 29 in in IN cord-312431-de7zhswl 293 30 Groundwaters Groundwaters NNP cord-312431-de7zhswl 293 31 Comparison Comparison NNP cord-312431-de7zhswl 293 32 of of IN cord-312431-de7zhswl 293 33 Methods Methods NNPS cord-312431-de7zhswl 293 34 for for IN cord-312431-de7zhswl 293 35 Detecting detect VBG cord-312431-de7zhswl 293 36 Genotypes Genotypes NNP cord-312431-de7zhswl 293 37 of of IN cord-312431-de7zhswl 293 38 F F NNP cord-312431-de7zhswl 293 39 - - HYPH cord-312431-de7zhswl 293 40 specific specific JJ cord-312431-de7zhswl 293 41 RNA RNA NNP cord-312431-de7zhswl 293 42 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 293 43 and and CC cord-312431-de7zhswl 293 44 Fingerprinting Fingerprinting NNP cord-312431-de7zhswl 293 45 the the DT cord-312431-de7zhswl 293 46 Origin Origin NNP cord-312431-de7zhswl 293 47 of of IN cord-312431-de7zhswl 293 48 Faecal Faecal NNP cord-312431-de7zhswl 293 49 Pollution Pollution NNP cord-312431-de7zhswl 293 50 in in IN cord-312431-de7zhswl 293 51 Water Water NNP cord-312431-de7zhswl 293 52 Samples Samples NNPS cord-312431-de7zhswl 293 53 Evaluation Evaluation NNP cord-312431-de7zhswl 293 54 of of IN cord-312431-de7zhswl 293 55 the the DT cord-312431-de7zhswl 293 56 Application Application NNP cord-312431-de7zhswl 293 57 of of IN cord-312431-de7zhswl 293 58 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 293 59 as as IN cord-312431-de7zhswl 293 60 Indicators Indicators NNP cord-312431-de7zhswl 293 61 of of IN cord-312431-de7zhswl 293 62 Water Water NNP cord-312431-de7zhswl 293 63 Quality Quality NNP cord-312431-de7zhswl 293 64 Comparison Comparison NNP cord-312431-de7zhswl 293 65 of of IN cord-312431-de7zhswl 293 66 F F NNP cord-312431-de7zhswl 293 67 - - HYPH cord-312431-de7zhswl 293 68 specific specific JJ cord-312431-de7zhswl 293 69 Bacteriophage Bacteriophage NNP cord-312431-de7zhswl 293 70 , , , cord-312431-de7zhswl 293 71 Enterococci Enterococci NNP cord-312431-de7zhswl 293 72 , , , cord-312431-de7zhswl 293 73 and and CC cord-312431-de7zhswl 293 74 Faecal Faecal NNP cord-312431-de7zhswl 293 75 Coliform Coliform NNP cord-312431-de7zhswl 293 76 Densities Densities NNPS cord-312431-de7zhswl 293 77 through through IN cord-312431-de7zhswl 293 78 Wastewater Wastewater NNP cord-312431-de7zhswl 293 79 Treatment Treatment NNP cord-312431-de7zhswl 293 80 Process Process NNP cord-312431-de7zhswl 294 1 Employing employ VBG cord-312431-de7zhswl 294 2 Oxidation oxidation NN cord-312431-de7zhswl 294 3 Ponds pond NNS cord-312431-de7zhswl 295 1 Malespecific Malespecific NNP cord-312431-de7zhswl 295 2 RNA RNA NNP cord-312431-de7zhswl 295 3 Coliphages Coliphages NNP cord-312431-de7zhswl 295 4 Detected detect VBN cord-312431-de7zhswl 295 5 by by IN cord-312431-de7zhswl 295 6 Plaque Plaque NNP cord-312431-de7zhswl 295 7 Assay Assay NNP cord-312431-de7zhswl 295 8 and and CC cord-312431-de7zhswl 295 9 RT RT NNP cord-312431-de7zhswl 295 10 - - HYPH cord-312431-de7zhswl 295 11 PCR PCR NNP cord-312431-de7zhswl 295 12 in in IN cord-312431-de7zhswl 295 13 Tropical Tropical NNP cord-312431-de7zhswl 295 14 River River NNP cord-312431-de7zhswl 295 15 Waters Waters NNPS cord-312431-de7zhswl 295 16 and and CC cord-312431-de7zhswl 295 17 Animal Animal NNP cord-312431-de7zhswl 295 18 Faecal Faecal NNP cord-312431-de7zhswl 295 19 Matter Matter NNP cord-312431-de7zhswl 295 20 Detection Detection NNP cord-312431-de7zhswl 295 21 of of IN cord-312431-de7zhswl 295 22 Viral viral JJ cord-312431-de7zhswl 295 23 Pathogens Pathogens NNPS cord-312431-de7zhswl 295 24 by by IN cord-312431-de7zhswl 295 25 Reverse Reverse NNP cord-312431-de7zhswl 295 26 Transcriptase Transcriptase NNP cord-312431-de7zhswl 295 27 PCR PCR NNP cord-312431-de7zhswl 295 28 and and CC cord-312431-de7zhswl 295 29 of of IN cord-312431-de7zhswl 295 30 Microbial Microbial NNP cord-312431-de7zhswl 295 31 Indicators Indicators NNPS cord-312431-de7zhswl 295 32 by by IN cord-312431-de7zhswl 295 33 Standard Standard NNP cord-312431-de7zhswl 295 34 Methods Methods NNPS cord-312431-de7zhswl 295 35 in in IN cord-312431-de7zhswl 295 36 the the DT cord-312431-de7zhswl 295 37 Canals Canals NNPS cord-312431-de7zhswl 295 38 of of IN cord-312431-de7zhswl 295 39 the the DT cord-312431-de7zhswl 295 40 Florida Florida NNP cord-312431-de7zhswl 295 41 Keys Keys NNP cord-312431-de7zhswl 295 42 Human Human NNP cord-312431-de7zhswl 295 43 Enteric Enteric NNP cord-312431-de7zhswl 295 44 Viruses Viruses NNPS cord-312431-de7zhswl 295 45 and and CC cord-312431-de7zhswl 295 46 Parasites parasite NNS cord-312431-de7zhswl 295 47 in in IN cord-312431-de7zhswl 295 48 the the DT cord-312431-de7zhswl 295 49 Marine Marine NNP cord-312431-de7zhswl 295 50 Environment Environment NNP cord-312431-de7zhswl 295 51 Incidence Incidence NNP cord-312431-de7zhswl 295 52 of of IN cord-312431-de7zhswl 295 53 Adenoviruses adenovirus NNS cord-312431-de7zhswl 295 54 in in IN cord-312431-de7zhswl 295 55 Raw Raw NNP cord-312431-de7zhswl 295 56 and and CC cord-312431-de7zhswl 295 57 Treated Treated NNP cord-312431-de7zhswl 295 58 Water Water NNP cord-312431-de7zhswl 295 59 Quantification Quantification NNP cord-312431-de7zhswl 295 60 and and CC cord-312431-de7zhswl 295 61 Typing typing NN cord-312431-de7zhswl 295 62 of of IN cord-312431-de7zhswl 295 63 Adenoviruses adenovirus NNS cord-312431-de7zhswl 295 64 Detected detect VBN cord-312431-de7zhswl 295 65 in in IN cord-312431-de7zhswl 295 66 River River NNP cord-312431-de7zhswl 295 67 and and CC cord-312431-de7zhswl 295 68 Treated Treated NNP cord-312431-de7zhswl 295 69 Drinking Drinking NNP cord-312431-de7zhswl 295 70 Water Water NNP cord-312431-de7zhswl 295 71 in in IN cord-312431-de7zhswl 295 72 South South NNP cord-312431-de7zhswl 295 73 Africa Africa NNP cord-312431-de7zhswl 295 74 Presence Presence NNP cord-312431-de7zhswl 295 75 of of IN cord-312431-de7zhswl 295 76 Noroviruses Noroviruses NNP cord-312431-de7zhswl 295 77 and and CC cord-312431-de7zhswl 295 78 Other other JJ cord-312431-de7zhswl 295 79 Enteric enteric JJ cord-312431-de7zhswl 295 80 Viruses Viruses NNPS cord-312431-de7zhswl 295 81 in in IN cord-312431-de7zhswl 295 82 Sewage sewage NN cord-312431-de7zhswl 295 83 and and CC cord-312431-de7zhswl 295 84 Surface Surface NNP cord-312431-de7zhswl 295 85 Waters Waters NNPS cord-312431-de7zhswl 295 86 in in IN cord-312431-de7zhswl 295 87 The the DT cord-312431-de7zhswl 295 88 Netherlands Netherlands NNP cord-312431-de7zhswl 295 89 Enteric enteric JJ cord-312431-de7zhswl 295 90 Viruses virus NNS cord-312431-de7zhswl 295 91 of of IN cord-312431-de7zhswl 295 92 Humans Humans NNPS cord-312431-de7zhswl 295 93 and and CC cord-312431-de7zhswl 295 94 Animals Animals NNPS cord-312431-de7zhswl 295 95 in in IN cord-312431-de7zhswl 295 96 Aquatic Aquatic NNP cord-312431-de7zhswl 295 97 Environments environment NNS cord-312431-de7zhswl 295 98 : : : cord-312431-de7zhswl 295 99 Health health NN cord-312431-de7zhswl 295 100 Risks risk NNS cord-312431-de7zhswl 295 101 , , , cord-312431-de7zhswl 295 102 Detection Detection NNP cord-312431-de7zhswl 295 103 , , , cord-312431-de7zhswl 295 104 and and CC cord-312431-de7zhswl 295 105 Potential Potential NNP cord-312431-de7zhswl 295 106 Water Water NNP cord-312431-de7zhswl 295 107 Quality Quality NNP cord-312431-de7zhswl 295 108 Assessment Assessment NNP cord-312431-de7zhswl 295 109 Tools Tools NNPS cord-312431-de7zhswl 295 110 , , , cord-312431-de7zhswl 295 111 Microbiol Microbiol NNP cord-312431-de7zhswl 295 112 Gastroenteritis Gastroenteritis NNP cord-312431-de7zhswl 295 113 Outbreak outbreak NN cord-312431-de7zhswl 295 114 Caused cause VBN cord-312431-de7zhswl 295 115 by by IN cord-312431-de7zhswl 295 116 Waterborne Waterborne NNP cord-312431-de7zhswl 295 117 Norovirus Norovirus NNP cord-312431-de7zhswl 295 118 at at IN cord-312431-de7zhswl 295 119 a a DT cord-312431-de7zhswl 295 120 New New NNP cord-312431-de7zhswl 295 121 Zealand Zealand NNP cord-312431-de7zhswl 295 122 Ski Ski NNP cord-312431-de7zhswl 295 123 Resort Resort NNP cord-312431-de7zhswl 295 124 A a DT cord-312431-de7zhswl 295 125 Waterborne waterborne JJ cord-312431-de7zhswl 295 126 Outbreak outbreak NN cord-312431-de7zhswl 295 127 of of IN cord-312431-de7zhswl 295 128 Gastroenteritis Gastroenteritis NNP cord-312431-de7zhswl 295 129 with with IN cord-312431-de7zhswl 295 130 Multiple Multiple NNP cord-312431-de7zhswl 295 131 Etiologies Etiologies NNPS cord-312431-de7zhswl 295 132 among among IN cord-312431-de7zhswl 295 133 Resort Resort NNP cord-312431-de7zhswl 295 134 Island Island NNP cord-312431-de7zhswl 295 135 Visitors Visitors NNPS cord-312431-de7zhswl 295 136 and and CC cord-312431-de7zhswl 295 137 Residents resident NNS cord-312431-de7zhswl 295 138 : : : cord-312431-de7zhswl 296 1 Ohio Ohio NNP cord-312431-de7zhswl 296 2 Human Human NNP cord-312431-de7zhswl 296 3 Adenoviruses Adenoviruses NNPS cord-312431-de7zhswl 296 4 and and CC cord-312431-de7zhswl 296 5 Coliphages Coliphages NNPS cord-312431-de7zhswl 296 6 in in IN cord-312431-de7zhswl 296 7 Urban Urban NNP cord-312431-de7zhswl 296 8 Runoff Runoff NNP cord-312431-de7zhswl 296 9 - - HYPH cord-312431-de7zhswl 296 10 Impacted impact VBN cord-312431-de7zhswl 296 11 Coastal Coastal NNP cord-312431-de7zhswl 296 12 Waters Waters NNPS cord-312431-de7zhswl 296 13 of of IN cord-312431-de7zhswl 296 14 Southern Southern NNP cord-312431-de7zhswl 296 15 California California NNP cord-312431-de7zhswl 296 16 Filtration Filtration NNP cord-312431-de7zhswl 296 17 - - HYPH cord-312431-de7zhswl 296 18 Based base VBN cord-312431-de7zhswl 296 19 Methods Methods NNPS cord-312431-de7zhswl 296 20 for for IN cord-312431-de7zhswl 296 21 the the DT cord-312431-de7zhswl 296 22 Collection Collection NNP cord-312431-de7zhswl 296 23 of of IN cord-312431-de7zhswl 296 24 Viral Viral NNP cord-312431-de7zhswl 296 25 Concentrates concentrate VBZ cord-312431-de7zhswl 296 26 from from IN cord-312431-de7zhswl 296 27 Large large JJ cord-312431-de7zhswl 296 28 Water water NN cord-312431-de7zhswl 296 29 Samples sample NNS cord-312431-de7zhswl 297 1 Virioplankton Virioplankton NNP cord-312431-de7zhswl 297 2 ' ' `` cord-312431-de7zhswl 297 3 Pegylation pegylation NN cord-312431-de7zhswl 297 4 ' ' '' cord-312431-de7zhswl 297 5 : : : cord-312431-de7zhswl 297 6 Use Use NNP cord-312431-de7zhswl 297 7 of of IN cord-312431-de7zhswl 297 8 PEG PEG NNP cord-312431-de7zhswl 297 9 ( ( -LRB- cord-312431-de7zhswl 297 10 polyethylene polyethylene NN cord-312431-de7zhswl 297 11 glycol glycol NN cord-312431-de7zhswl 297 12 ) ) -RRB- cord-312431-de7zhswl 297 13 to to IN cord-312431-de7zhswl 297 14 Concentrate concentrate VB cord-312431-de7zhswl 297 15 and and CC cord-312431-de7zhswl 297 16 Purify purify VB cord-312431-de7zhswl 297 17 Viruses virus NNS cord-312431-de7zhswl 297 18 in in IN cord-312431-de7zhswl 297 19 Pelagic Pelagic NNP cord-312431-de7zhswl 297 20 Ecosystems Ecosystems NNPS cord-312431-de7zhswl 297 21 Application Application NNP cord-312431-de7zhswl 297 22 of of IN cord-312431-de7zhswl 297 23 Digital Digital NNP cord-312431-de7zhswl 297 24 Image Image NNP cord-312431-de7zhswl 297 25 Analysis Analysis NNP cord-312431-de7zhswl 297 26 and and CC cord-312431-de7zhswl 297 27 Flow flow VB cord-312431-de7zhswl 297 28 Cytometry Cytometry NNP cord-312431-de7zhswl 297 29 to to TO cord-312431-de7zhswl 297 30 Enumerate enumerate VB cord-312431-de7zhswl 297 31 Marine marine NN cord-312431-de7zhswl 297 32 Viruses virus NNS cord-312431-de7zhswl 297 33 Stained stain VBN cord-312431-de7zhswl 297 34 with with IN cord-312431-de7zhswl 297 35 SYBR SYBR NNP cord-312431-de7zhswl 297 36 Gold Gold NNP cord-312431-de7zhswl 297 37 Comparison Comparison NNP cord-312431-de7zhswl 297 38 of of IN cord-312431-de7zhswl 297 39 SYBR SYBR NNP cord-312431-de7zhswl 297 40 Green Green NNP cord-312431-de7zhswl 297 41 I I NNP cord-312431-de7zhswl 297 42 and and CC cord-312431-de7zhswl 297 43 SYBR SYBR NNP cord-312431-de7zhswl 297 44 Gold gold NN cord-312431-de7zhswl 297 45 Stains stain NNS cord-312431-de7zhswl 297 46 for for IN cord-312431-de7zhswl 297 47 Enumerating enumerate VBG cord-312431-de7zhswl 297 48 Bacteria Bacteria NNS cord-312431-de7zhswl 297 49 and and CC cord-312431-de7zhswl 297 50 Viruses virus NNS cord-312431-de7zhswl 297 51 by by IN cord-312431-de7zhswl 297 52 Epifluorescence Epifluorescence NNP cord-312431-de7zhswl 297 53 Microscopy Microscopy NNP cord-312431-de7zhswl 297 54 Virus Virus NNP cord-312431-de7zhswl 297 55 and and CC cord-312431-de7zhswl 297 56 Prokaryote Prokaryote NNP cord-312431-de7zhswl 297 57 Enumeration enumeration NN cord-312431-de7zhswl 297 58 from from IN cord-312431-de7zhswl 297 59 Planktonic Planktonic NNP cord-312431-de7zhswl 297 60 Aquatic Aquatic NNP cord-312431-de7zhswl 297 61 Environments Environments NNPS cord-312431-de7zhswl 297 62 by by IN cord-312431-de7zhswl 297 63 Epifluorescence Epifluorescence NNP cord-312431-de7zhswl 297 64 Microscopy Microscopy NNP cord-312431-de7zhswl 297 65 with with IN cord-312431-de7zhswl 297 66 SYBR SYBR NNP cord-312431-de7zhswl 297 67 Green Green NNP cord-312431-de7zhswl 298 1 I -PRON- PRP cord-312431-de7zhswl 298 2 Metagenomic Metagenomic NNP cord-312431-de7zhswl 298 3 Analysis Analysis NNP cord-312431-de7zhswl 298 4 of of IN cord-312431-de7zhswl 298 5 Viruses Viruses NNPS cord-312431-de7zhswl 298 6 in in IN cord-312431-de7zhswl 298 7 Reclaimed Reclaimed NNP cord-312431-de7zhswl 298 8 Water Water NNP cord-312431-de7zhswl 298 9 EPA EPA NNP cord-312431-de7zhswl 298 10 Groundwater Groundwater NNP cord-312431-de7zhswl 298 11 and and CC cord-312431-de7zhswl 298 12 Drinking Drinking NNP cord-312431-de7zhswl 298 13 Water Water NNP cord-312431-de7zhswl 298 14 Current Current NNP cord-312431-de7zhswl 298 15 Standards Standards NNPS cord-312431-de7zhswl 298 16 A A NNP cord-312431-de7zhswl 298 17 Colorimetric colorimetric JJ cord-312431-de7zhswl 298 18 Assay Assay NNP cord-312431-de7zhswl 298 19 for for IN cord-312431-de7zhswl 298 20 Viral Viral NNP cord-312431-de7zhswl 298 21 Agents Agents NNPS cord-312431-de7zhswl 298 22 that that WDT cord-312431-de7zhswl 298 23 Produce produce VBP cord-312431-de7zhswl 298 24 Cytopathic cytopathic JJ cord-312431-de7zhswl 298 25 Effects effect NNS cord-312431-de7zhswl 298 26 Systat Systat NNP cord-312431-de7zhswl 298 27 : : : cord-312431-de7zhswl 299 1 The the DT cord-312431-de7zhswl 299 2 System System NNP cord-312431-de7zhswl 299 3 for for IN cord-312431-de7zhswl 299 4 Statistics Statistics NNP cord-312431-de7zhswl 299 5 Relationships Relationships NNPS cord-312431-de7zhswl 299 6 Between between IN cord-312431-de7zhswl 299 7 Benthic Benthic NNP cord-312431-de7zhswl 299 8 Biota Biota NNP cord-312431-de7zhswl 299 9 and and CC cord-312431-de7zhswl 299 10 Hydrological Hydrological NNP cord-312431-de7zhswl 299 11 Indices Indices NNPS cord-312431-de7zhswl 299 12 in in IN cord-312431-de7zhswl 299 13 New New NNP cord-312431-de7zhswl 299 14 Zealand Zealand NNP cord-312431-de7zhswl 299 15 Streams Streams NNPS cord-312431-de7zhswl 299 16 , , , cord-312431-de7zhswl 299 17 Freshwater Freshwater NNP cord-312431-de7zhswl 299 18 Biol Biol NNP cord-312431-de7zhswl 299 19 Canonical Canonical NNP cord-312431-de7zhswl 299 20 Correspondence Correspondence NNP cord-312431-de7zhswl 299 21 Analysis Analysis NNP cord-312431-de7zhswl 299 22 and and CC cord-312431-de7zhswl 299 23 Related Related NNP cord-312431-de7zhswl 299 24 Multivariate Multivariate NNP cord-312431-de7zhswl 299 25 Methods Methods NNPS cord-312431-de7zhswl 299 26 in in IN cord-312431-de7zhswl 299 27 Aquatic aquatic JJ cord-312431-de7zhswl 299 28 Ecology Ecology NNP cord-312431-de7zhswl 300 1 The the DT cord-312431-de7zhswl 300 2 Present Present NNP cord-312431-de7zhswl 300 3 State State NNP cord-312431-de7zhswl 300 4 of of IN cord-312431-de7zhswl 300 5 Phage Phage NNP cord-312431-de7zhswl 300 6 Taxonomy Taxonomy NNP cord-312431-de7zhswl 300 7 Basic basic JJ cord-312431-de7zhswl 300 8 electron electron NN cord-312431-de7zhswl 300 9 microscopy microscopy NN cord-312431-de7zhswl 300 10 of of IN cord-312431-de7zhswl 300 11 aquatic aquatic JJ cord-312431-de7zhswl 300 12 viruses virus NNS cord-312431-de7zhswl 300 13 Use use VBP cord-312431-de7zhswl 300 14 of of IN cord-312431-de7zhswl 300 15 Transmission Transmission NNP cord-312431-de7zhswl 300 16 Electron Electron NNP cord-312431-de7zhswl 300 17 Microscopy Microscopy NNP cord-312431-de7zhswl 300 18 for for IN cord-312431-de7zhswl 300 19 Viral Viral NNP cord-312431-de7zhswl 300 20 Diagnosis Diagnosis NNP cord-312431-de7zhswl 300 21 in in IN cord-312431-de7zhswl 300 22 Psittacine Psittacine NNP cord-312431-de7zhswl 300 23 Birds Birds NNPS cord-312431-de7zhswl 300 24 , , , cord-312431-de7zhswl 300 25 International International NNP cord-312431-de7zhswl 300 26 Virtual Virtual NNP cord-312431-de7zhswl 300 27 Conferences Conferences NNPS cord-312431-de7zhswl 300 28 in in IN cord-312431-de7zhswl 300 29 Veterinary Veterinary NNP cord-312431-de7zhswl 300 30 Medicine Medicine NNP cord-312431-de7zhswl 301 1 The the DT cord-312431-de7zhswl 301 2 Role role NN cord-312431-de7zhswl 301 3 of of IN cord-312431-de7zhswl 301 4 Electron Electron NNP cord-312431-de7zhswl 301 5 Microscopy Microscopy NNP cord-312431-de7zhswl 301 6 in in IN cord-312431-de7zhswl 301 7 the the DT cord-312431-de7zhswl 301 8 Rapid Rapid NNP cord-312431-de7zhswl 301 9 Diagnosis Diagnosis NNP cord-312431-de7zhswl 301 10 of of IN cord-312431-de7zhswl 301 11 Viral Viral NNP cord-312431-de7zhswl 301 12 Infections infection NNS cord-312431-de7zhswl 302 1 -Review -Review . cord-312431-de7zhswl 303 1 Modern modern JJ cord-312431-de7zhswl 303 2 Uses use NNS cord-312431-de7zhswl 303 3 of of IN cord-312431-de7zhswl 303 4 Electron Electron NNP cord-312431-de7zhswl 303 5 Microscopy Microscopy NNP cord-312431-de7zhswl 303 6 for for IN cord-312431-de7zhswl 303 7 Detection detection NN cord-312431-de7zhswl 303 8 of of IN cord-312431-de7zhswl 303 9 Viruses virus NNS cord-312431-de7zhswl 304 1 The the DT cord-312431-de7zhswl 304 2 role role NN cord-312431-de7zhswl 304 3 of of IN cord-312431-de7zhswl 304 4 electron electron NN cord-312431-de7zhswl 304 5 microscopy microscopy NN cord-312431-de7zhswl 304 6 in in IN cord-312431-de7zhswl 304 7 the the DT cord-312431-de7zhswl 304 8 detection detection NN cord-312431-de7zhswl 304 9 and and CC cord-312431-de7zhswl 304 10 characterisation characterisation NN cord-312431-de7zhswl 304 11 of of IN cord-312431-de7zhswl 304 12 viruses virus NNS cord-312431-de7zhswl 304 13 associated associate VBN cord-312431-de7zhswl 304 14 with with IN cord-312431-de7zhswl 304 15 gastroenteritis gastroenteritis NN cord-312431-de7zhswl 304 16 in in IN cord-312431-de7zhswl 304 17 humans human NNS cord-312431-de7zhswl 305 1 Negative negative JJ cord-312431-de7zhswl 305 2 Stain stain NN cord-312431-de7zhswl 305 3 Transmission transmission NN cord-312431-de7zhswl 305 4 Electron electron NN cord-312431-de7zhswl 305 5 Microscopy Microscopy NNP cord-312431-de7zhswl 305 6 Quality quality NN cord-312431-de7zhswl 305 7 , , , cord-312431-de7zhswl 305 8 Microscope Microscope NNP cord-312431-de7zhswl 305 9 Microanal Microanal NNP cord-312431-de7zhswl 306 1 The the DT cord-312431-de7zhswl 306 2 Role role NN cord-312431-de7zhswl 306 3 of of IN cord-312431-de7zhswl 306 4 Transmission Transmission NNP cord-312431-de7zhswl 306 5 Electron Electron NNP cord-312431-de7zhswl 306 6 Microscopy Microscopy NNP cord-312431-de7zhswl 306 7 in in IN cord-312431-de7zhswl 306 8 the the DT cord-312431-de7zhswl 306 9 Study Study NNP cord-312431-de7zhswl 306 10 of of IN cord-312431-de7zhswl 306 11 Gastroenteritis Gastroenteritis NNP cord-312431-de7zhswl 306 12 Viruses Viruses NNPS cord-312431-de7zhswl 306 13 Determination determination NN cord-312431-de7zhswl 306 14 of of IN cord-312431-de7zhswl 306 15 Enteroviruses Enteroviruses NNP cord-312431-de7zhswl 306 16 , , , cord-312431-de7zhswl 306 17 Hepatitis Hepatitis NNP cord-312431-de7zhswl 306 18 A A NNP cord-312431-de7zhswl 306 19 Virus virus NN cord-312431-de7zhswl 306 20 , , , cord-312431-de7zhswl 306 21 Bacteriophages Bacteriophages NNPS cord-312431-de7zhswl 306 22 and and CC cord-312431-de7zhswl 306 23 Escherichia Escherichia NNP cord-312431-de7zhswl 306 24 coli coli NNS cord-312431-de7zhswl 306 25 in in IN cord-312431-de7zhswl 306 26 Adriatic Adriatic NNP cord-312431-de7zhswl 306 27 Sea Sea NNP cord-312431-de7zhswl 306 28 Mussels Mussels NNPS cord-312431-de7zhswl 306 29 Occurrence occurrence NN cord-312431-de7zhswl 306 30 of of IN cord-312431-de7zhswl 306 31 Bacteria Bacteria NNS cord-312431-de7zhswl 306 32 and and CC cord-312431-de7zhswl 306 33 Coli Coli NNP cord-312431-de7zhswl 306 34 Bacteriophages bacteriophage NNS cord-312431-de7zhswl 306 35 as as IN cord-312431-de7zhswl 306 36 Potential potential JJ cord-312431-de7zhswl 306 37 Indicators Indicators NNPS cord-312431-de7zhswl 306 38 of of IN cord-312431-de7zhswl 306 39 Faecal Faecal NNP cord-312431-de7zhswl 306 40 Pollution Pollution NNP cord-312431-de7zhswl 306 41 of of IN cord-312431-de7zhswl 306 42 Vistula Vistula NNP cord-312431-de7zhswl 306 43 River River NNP cord-312431-de7zhswl 306 44 and and CC cord-312431-de7zhswl 306 45 Zegrze Zegrze NNP cord-312431-de7zhswl 306 46 Reservoir Reservoir NNP cord-312431-de7zhswl 306 47 Incidence Incidence NNP cord-312431-de7zhswl 306 48 of of IN cord-312431-de7zhswl 306 49 Coliphage Coliphage NNP cord-312431-de7zhswl 306 50 in in IN cord-312431-de7zhswl 306 51 Potable Potable NNP cord-312431-de7zhswl 306 52 Water Water NNP cord-312431-de7zhswl 306 53 Supplies Supplies NNPS cord-312431-de7zhswl 306 54 Distribution distribution NN cord-312431-de7zhswl 306 55 Comparison comparison NN cord-312431-de7zhswl 306 56 between between IN cord-312431-de7zhswl 306 57 Coliphages Coliphages NNP cord-312431-de7zhswl 306 58 and and CC cord-312431-de7zhswl 306 59 Phages Phages NNPS cord-312431-de7zhswl 306 60 of of IN cord-312431-de7zhswl 306 61 Anaerobic anaerobic JJ cord-312431-de7zhswl 306 62 Bacteria Bacteria NNS cord-312431-de7zhswl 306 63 ( ( -LRB- cord-312431-de7zhswl 306 64 Bacteroides Bacteroides NNP cord-312431-de7zhswl 306 65 fragilis fragili NNS cord-312431-de7zhswl 306 66 ) ) -RRB- cord-312431-de7zhswl 306 67 in in IN cord-312431-de7zhswl 306 68 Water Water NNP cord-312431-de7zhswl 306 69 Sources Sources NNPS cord-312431-de7zhswl 306 70 and and CC cord-312431-de7zhswl 306 71 Their -PRON- PRP$ cord-312431-de7zhswl 306 72 Reliability Reliability NNP cord-312431-de7zhswl 306 73 as as IN cord-312431-de7zhswl 306 74 Faecal Faecal NNP cord-312431-de7zhswl 306 75 Pollution Pollution NNP cord-312431-de7zhswl 306 76 Indicators Indicators NNPS cord-312431-de7zhswl 306 77 in in IN cord-312431-de7zhswl 306 78 Drinking Drinking NNP cord-312431-de7zhswl 306 79 Water Water NNP cord-312431-de7zhswl 306 80 Alternative Alternative NNP cord-312431-de7zhswl 306 81 Methods Methods NNPS cord-312431-de7zhswl 306 82 in in IN cord-312431-de7zhswl 306 83 Tracking Tracking NNP cord-312431-de7zhswl 306 84 Sources source NNS cord-312431-de7zhswl 306 85 of of IN cord-312431-de7zhswl 306 86 Microbial microbial JJ cord-312431-de7zhswl 306 87 Contamination Contamination NNP cord-312431-de7zhswl 306 88 in in IN cord-312431-de7zhswl 306 89 Waters Waters NNP cord-312431-de7zhswl 306 90 , , , cord-312431-de7zhswl 306 91 Water Water NNP cord-312431-de7zhswl 306 92 SA SA NNP cord-312431-de7zhswl 306 93 National National NNP cord-312431-de7zhswl 306 94 Water Water NNP cord-312431-de7zhswl 306 95 Resource Resource NNP cord-312431-de7zhswl 306 96 Strategy Strategy NNP cord-312431-de7zhswl 306 97 ( ( -LRB- cord-312431-de7zhswl 306 98 NWRS NWRS NNP cord-312431-de7zhswl 306 99 ) ) -RRB- cord-312431-de7zhswl 306 100 , , , cord-312431-de7zhswl 306 101 1st 1st NN cord-312431-de7zhswl 306 102 Ed Ed NNP cord-312431-de7zhswl 306 103 . . NNP cord-312431-de7zhswl 306 104 , , , cord-312431-de7zhswl 306 105 Department Department NNP cord-312431-de7zhswl 306 106 of of IN cord-312431-de7zhswl 306 107 Water Water NNP cord-312431-de7zhswl 306 108 Affairs Affairs NNPS cord-312431-de7zhswl 306 109 and and CC cord-312431-de7zhswl 306 110 Forestry Forestry NNP cord-312431-de7zhswl 306 111 Antibiotic Antibiotic NNP cord-312431-de7zhswl 306 112 Resistance Resistance NNP cord-312431-de7zhswl 306 113 Profiles Profiles NNPS cord-312431-de7zhswl 306 114 of of IN cord-312431-de7zhswl 306 115 Escherichia Escherichia NNP cord-312431-de7zhswl 306 116 coli coli VBZ cord-312431-de7zhswl 306 117 Isolates Isolates NNPS cord-312431-de7zhswl 306 118 from from IN cord-312431-de7zhswl 306 119 River River NNP cord-312431-de7zhswl 306 120 Sources Sources NNPS cord-312431-de7zhswl 306 121 in in IN cord-312431-de7zhswl 306 122 Durban Durban NNP cord-312431-de7zhswl 306 123 Methods Methods NNPS cord-312431-de7zhswl 306 124 for for IN cord-312431-de7zhswl 306 125 Studying study VBG cord-312431-de7zhswl 306 126 Aquatic Aquatic NNP cord-312431-de7zhswl 306 127 Bacteriophage Bacteriophage NNP cord-312431-de7zhswl 306 128 Ecology Ecology NNP cord-312431-de7zhswl 306 129 Enumeration enumeration NN cord-312431-de7zhswl 306 130 and and CC cord-312431-de7zhswl 306 131 Isolation isolation NN cord-312431-de7zhswl 306 132 of of IN cord-312431-de7zhswl 306 133 Viral Viral NNP cord-312431-de7zhswl 306 134 Particles Particles NNPS cord-312431-de7zhswl 306 135 from from IN cord-312431-de7zhswl 306 136 Oligotrophic Oligotrophic NNP cord-312431-de7zhswl 306 137 Marine Marine NNP cord-312431-de7zhswl 306 138 Environments environment NNS cord-312431-de7zhswl 306 139 by by IN cord-312431-de7zhswl 306 140 Tangential Tangential NNP cord-312431-de7zhswl 306 141 Flow Flow NNP cord-312431-de7zhswl 306 142 Filtration filtration NN cord-312431-de7zhswl 306 143 Bacteriophage bacteriophage NN cord-312431-de7zhswl 306 144 Electron electron NN cord-312431-de7zhswl 306 145 Microscopy Microscopy NNP cord-312431-de7zhswl 306 146 Direct direct JJ cord-312431-de7zhswl 306 147 Electron electron NN cord-312431-de7zhswl 306 148 Microscopy Microscopy NNP cord-312431-de7zhswl 306 149 Study Study NNP cord-312431-de7zhswl 306 150 on on IN cord-312431-de7zhswl 306 151 the the DT cord-312431-de7zhswl 306 152 Morphological morphological JJ cord-312431-de7zhswl 306 153 Diversity diversity NN cord-312431-de7zhswl 306 154 of of IN cord-312431-de7zhswl 306 155 Bacteriophage Bacteriophage NNP cord-312431-de7zhswl 306 156 Populations Populations NNPS cord-312431-de7zhswl 306 157 in in IN cord-312431-de7zhswl 306 158 Lake Lake NNP cord-312431-de7zhswl 306 159 PluBsee PluBsee NNP cord-312431-de7zhswl 306 160 Rapid Rapid NNP cord-312431-de7zhswl 306 161 Diagnosis Diagnosis NNP cord-312431-de7zhswl 306 162 of of IN cord-312431-de7zhswl 306 163 Plant Plant NNP cord-312431-de7zhswl 306 164 Virus virus NN cord-312431-de7zhswl 306 165 Diseases Diseases NNPS cord-312431-de7zhswl 306 166 by by IN cord-312431-de7zhswl 306 167 Transmission Transmission NNP cord-312431-de7zhswl 306 168 Electron electron NN cord-312431-de7zhswl 306 169 Microscopy Microscopy NNP cord-312431-de7zhswl 306 170 Abundance abundance NN cord-312431-de7zhswl 306 171 of of IN cord-312431-de7zhswl 306 172 Viruses virus NNS cord-312431-de7zhswl 306 173 in in IN cord-312431-de7zhswl 306 174 Marine Marine NNP cord-312431-de7zhswl 306 175 Waters Waters NNPS cord-312431-de7zhswl 306 176 : : : cord-312431-de7zhswl 307 1 Assessment assessment NN cord-312431-de7zhswl 307 2 by by IN cord-312431-de7zhswl 307 3 Epifluorescence Epifluorescence NNP cord-312431-de7zhswl 307 4 and and CC cord-312431-de7zhswl 307 5 Transmission Transmission NNP cord-312431-de7zhswl 307 6 Electron Electron NNP cord-312431-de7zhswl 307 7 Microscopy Microscopy NNP cord-312431-de7zhswl 307 8 Frequency Frequency NNP cord-312431-de7zhswl 307 9 of of IN cord-312431-de7zhswl 307 10 Morphological morphological JJ cord-312431-de7zhswl 307 11 Phage phage NN cord-312431-de7zhswl 307 12 Description description NN cord-312431-de7zhswl 307 13 Distribution distribution NN cord-312431-de7zhswl 307 14 of of IN cord-312431-de7zhswl 307 15 Viruses Viruses NNPS cord-312431-de7zhswl 307 16 in in IN cord-312431-de7zhswl 307 17 the the DT cord-312431-de7zhswl 307 18 Chesapeake Chesapeake NNP cord-312431-de7zhswl 307 19 Bay Bay NNP cord-312431-de7zhswl 307 20 Human Human NNP cord-312431-de7zhswl 307 21 Adenoviruses Adenoviruses NNPS cord-312431-de7zhswl 307 22 in in IN cord-312431-de7zhswl 307 23 Water water NN cord-312431-de7zhswl 307 24 : : : cord-312431-de7zhswl 308 1 Occurrence occurrence NN cord-312431-de7zhswl 308 2 and and CC cord-312431-de7zhswl 308 3 Health Health NNP cord-312431-de7zhswl 308 4 Implications Implications NNPS cord-312431-de7zhswl 308 5 : : : cord-312431-de7zhswl 309 1 A a DT cord-312431-de7zhswl 309 2 Critical Critical NNP cord-312431-de7zhswl 309 3 Review Review NNP cord-312431-de7zhswl 309 4 Enteric Enteric NNP cord-312431-de7zhswl 309 5 Virus virus NN cord-312431-de7zhswl 309 6 Isolation isolation NN cord-312431-de7zhswl 309 7 in in IN cord-312431-de7zhswl 309 8 Different different JJ cord-312431-de7zhswl 309 9 Cell cell NN cord-312431-de7zhswl 309 10 Cultures culture NNS cord-312431-de7zhswl 310 1 The the DT cord-312431-de7zhswl 310 2 Role role NN cord-312431-de7zhswl 310 3 of of IN cord-312431-de7zhswl 310 4 Polyomaviruses Polyomaviruses NNPS cord-312431-de7zhswl 310 5 in in IN cord-312431-de7zhswl 310 6 Human Human NNP cord-312431-de7zhswl 310 7 Disease Disease NNP cord-312431-de7zhswl 310 8 Propagation Propagation NNP cord-312431-de7zhswl 310 9 of of IN cord-312431-de7zhswl 310 10 Adenovirus Adenovirus NNP cord-312431-de7zhswl 310 11 Type Type NNP cord-312431-de7zhswl 310 12 40 40 CD cord-312431-de7zhswl 310 13 and and CC cord-312431-de7zhswl 310 14 41 41 CD cord-312431-de7zhswl 310 15 in in IN cord-312431-de7zhswl 310 16 the the DT cord-312431-de7zhswl 310 17 PLC PLC NNP cord-312431-de7zhswl 310 18 / / SYM cord-312431-de7zhswl 310 19 PRF/5 PRF/5 NNP cord-312431-de7zhswl 310 20 Primary Primary NNP cord-312431-de7zhswl 310 21 Liver Liver NNP cord-312431-de7zhswl 310 22 Carcinoma Carcinoma NNP cord-312431-de7zhswl 310 23 Cell cell NN cord-312431-de7zhswl 310 24 Line line NN cord-312431-de7zhswl 310 25 Role role NN cord-312431-de7zhswl 310 26 of of IN cord-312431-de7zhswl 310 27 Cell cell NN cord-312431-de7zhswl 310 28 Culture Culture NNP cord-312431-de7zhswl 310 29 for for IN cord-312431-de7zhswl 310 30 Virus Virus NNP cord-312431-de7zhswl 310 31 Detection Detection NNP cord-312431-de7zhswl 310 32 in in IN cord-312431-de7zhswl 310 33 the the DT cord-312431-de7zhswl 310 34 Age Age NNP cord-312431-de7zhswl 310 35 of of IN cord-312431-de7zhswl 310 36 Technology Technology NNP cord-312431-de7zhswl 310 37 Yield Yield NNP cord-312431-de7zhswl 310 38 and and CC cord-312431-de7zhswl 310 39 Berry Berry NNP cord-312431-de7zhswl 310 40 Composition Composition NNP cord-312431-de7zhswl 310 41 of of IN cord-312431-de7zhswl 310 42 Five five CD cord-312431-de7zhswl 310 43 Vitis Vitis NNP cord-312431-de7zhswl 310 44 Cultivars Cultivars NNP cord-312431-de7zhswl 310 45 as as IN cord-312431-de7zhswl 310 46 Affected affect VBN cord-312431-de7zhswl 310 47 by by IN cord-312431-de7zhswl 310 48 Rupestris Rupestris NNP cord-312431-de7zhswl 310 49 Stem Stem NNP cord-312431-de7zhswl 310 50 Pitting pit VBG cord-312431-de7zhswl 310 51 Virus virus NN cord-312431-de7zhswl 310 52 Detecting detect VBG cord-312431-de7zhswl 310 53 Virus virus NN cord-312431-de7zhswl 311 1 -Like -Like NNP cord-312431-de7zhswl 311 2 Particles particle NNS cord-312431-de7zhswl 312 1 The the DT cord-312431-de7zhswl 312 2 authors author NNS cord-312431-de7zhswl 312 3 would would MD cord-312431-de7zhswl 312 4 like like VB cord-312431-de7zhswl 312 5 to to TO cord-312431-de7zhswl 312 6 thank thank VB cord-312431-de7zhswl 312 7 the the DT cord-312431-de7zhswl 312 8 Water Water NNP cord-312431-de7zhswl 312 9 Research Research NNP cord-312431-de7zhswl 312 10 Commission Commission NNP cord-312431-de7zhswl 312 11 of of IN cord-312431-de7zhswl 312 12 South South NNP cord-312431-de7zhswl 312 13 Africa Africa NNP cord-312431-de7zhswl 312 14 and and CC cord-312431-de7zhswl 312 15 the the DT cord-312431-de7zhswl 312 16 National National NNP cord-312431-de7zhswl 312 17 Research Research NNP cord-312431-de7zhswl 312 18 Foundation Foundation NNP cord-312431-de7zhswl 312 19 for for IN cord-312431-de7zhswl 312 20 funding funding NN cord-312431-de7zhswl 312 21 . . . cord-312431-de7zhswl 313 1 The the DT cord-312431-de7zhswl 313 2 authors author NNS cord-312431-de7zhswl 313 3 have have VBP cord-312431-de7zhswl 313 4 declared declare VBN cord-312431-de7zhswl 313 5 no no DT cord-312431-de7zhswl 313 6 conflict conflict NN cord-312431-de7zhswl 313 7 of of IN cord-312431-de7zhswl 313 8 interest interest NN cord-312431-de7zhswl 313 9 . . .