id sid tid token lemma pos cord-319100-3gdawhfn 1 1 key key JJ cord-319100-3gdawhfn 1 2 : : : cord-319100-3gdawhfn 1 3 cord-319100 cord-319100 NN cord-319100-3gdawhfn 1 4 - - HYPH cord-319100-3gdawhfn 1 5 3gdawhfn 3gdawhfn CD cord-319100-3gdawhfn 1 6 authors author NNS cord-319100-3gdawhfn 1 7 : : : cord-319100-3gdawhfn 1 8 Kirkland Kirkland NNP cord-319100-3gdawhfn 1 9 , , , cord-319100-3gdawhfn 1 10 P.D. P.D. NNP cord-319100-3gdawhfn 1 11 ; ; : cord-319100-3gdawhfn 1 12 Frost Frost NNP cord-319100-3gdawhfn 1 13 , , , cord-319100-3gdawhfn 2 1 M.J. M.J. NNP cord-319100-3gdawhfn 2 2 title title NN cord-319100-3gdawhfn 2 3 : : : cord-319100-3gdawhfn 3 1 The the DT cord-319100-3gdawhfn 3 2 impact impact NN cord-319100-3gdawhfn 3 3 of of IN cord-319100-3gdawhfn 3 4 viral viral JJ cord-319100-3gdawhfn 3 5 transport transport NN cord-319100-3gdawhfn 3 6 media medium NNS cord-319100-3gdawhfn 3 7 on on IN cord-319100-3gdawhfn 3 8 PCR PCR NNP cord-319100-3gdawhfn 3 9 assay assay NN cord-319100-3gdawhfn 3 10 results result VBZ cord-319100-3gdawhfn 3 11 for for IN cord-319100-3gdawhfn 3 12 the the DT cord-319100-3gdawhfn 3 13 detection detection NN cord-319100-3gdawhfn 3 14 of of IN cord-319100-3gdawhfn 3 15 nucleic nucleic JJ cord-319100-3gdawhfn 3 16 acid acid NN cord-319100-3gdawhfn 3 17 from from IN cord-319100-3gdawhfn 3 18 SARS SARS NNP cord-319100-3gdawhfn 3 19 - - HYPH cord-319100-3gdawhfn 3 20 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 3 21 and and CC cord-319100-3gdawhfn 3 22 other other JJ cord-319100-3gdawhfn 3 23 viruses virus NNS cord-319100-3gdawhfn 4 1 date date NN cord-319100-3gdawhfn 4 2 : : : cord-319100-3gdawhfn 4 3 2020 2020 CD cord-319100-3gdawhfn 4 4 - - HYPH cord-319100-3gdawhfn 4 5 06 06 CD cord-319100-3gdawhfn 4 6 - - SYM cord-319100-3gdawhfn 4 7 10 10 CD cord-319100-3gdawhfn 4 8 journal journal NN cord-319100-3gdawhfn 4 9 : : : cord-319100-3gdawhfn 5 1 bioRxiv biorxiv IN cord-319100-3gdawhfn 5 2 DOI DOI NNP cord-319100-3gdawhfn 5 3 : : : cord-319100-3gdawhfn 6 1 10.1101/2020.06.09.142323 10.1101/2020.06.09.142323 CD cord-319100-3gdawhfn 7 1 sha sha NNP cord-319100-3gdawhfn 7 2 : : : cord-319100-3gdawhfn 7 3 d900742851d847ecb31ac2db4d659d0071e8ba39 d900742851d847ecb31ac2db4d659d0071e8ba39 NNP cord-319100-3gdawhfn 7 4 doc_id doc_id CD cord-319100-3gdawhfn 7 5 : : : cord-319100-3gdawhfn 7 6 319100 319100 CD cord-319100-3gdawhfn 7 7 cord_uid cord_uid NNS cord-319100-3gdawhfn 7 8 : : : cord-319100-3gdawhfn 8 1 3gdawhfn 3gdawhfn LS cord-319100-3gdawhfn 8 2 During during IN cord-319100-3gdawhfn 8 3 the the DT cord-319100-3gdawhfn 8 4 2020 2020 CD cord-319100-3gdawhfn 8 5 SARS SARS NNP cord-319100-3gdawhfn 8 6 - - HYPH cord-319100-3gdawhfn 8 7 Cov-2 Cov-2 NNP cord-319100-3gdawhfn 8 8 pandemic pandemic NN cord-319100-3gdawhfn 8 9 , , , cord-319100-3gdawhfn 8 10 there there EX cord-319100-3gdawhfn 8 11 has have VBZ cord-319100-3gdawhfn 8 12 been be VBN cord-319100-3gdawhfn 8 13 an an DT cord-319100-3gdawhfn 8 14 acute acute JJ cord-319100-3gdawhfn 8 15 shortage shortage NN cord-319100-3gdawhfn 8 16 of of IN cord-319100-3gdawhfn 8 17 viral viral JJ cord-319100-3gdawhfn 8 18 transport transport NN cord-319100-3gdawhfn 8 19 medium medium NN cord-319100-3gdawhfn 8 20 . . . cord-319100-3gdawhfn 9 1 Many many JJ cord-319100-3gdawhfn 9 2 different different JJ cord-319100-3gdawhfn 9 3 products product NNS cord-319100-3gdawhfn 9 4 have have VBP cord-319100-3gdawhfn 9 5 been be VBN cord-319100-3gdawhfn 9 6 used use VBN cord-319100-3gdawhfn 9 7 to to TO cord-319100-3gdawhfn 9 8 meet meet VB cord-319100-3gdawhfn 9 9 the the DT cord-319100-3gdawhfn 9 10 demands demand NNS cord-319100-3gdawhfn 9 11 of of IN cord-319100-3gdawhfn 9 12 large large JJ cord-319100-3gdawhfn 9 13 - - HYPH cord-319100-3gdawhfn 9 14 scale scale NN cord-319100-3gdawhfn 9 15 diagnostic diagnostic JJ cord-319100-3gdawhfn 9 16 and and CC cord-319100-3gdawhfn 9 17 surveillance surveillance NN cord-319100-3gdawhfn 9 18 testing testing NN cord-319100-3gdawhfn 9 19 . . . cord-319100-3gdawhfn 10 1 The the DT cord-319100-3gdawhfn 10 2 stability stability NN cord-319100-3gdawhfn 10 3 of of IN cord-319100-3gdawhfn 10 4 SARS SARS NNP cord-319100-3gdawhfn 10 5 - - HYPH cord-319100-3gdawhfn 10 6 Cov-2 Cov-2 NNP cord-319100-3gdawhfn 10 7 RNA RNA NNP cord-319100-3gdawhfn 10 8 was be VBD cord-319100-3gdawhfn 10 9 assessed assess VBN cord-319100-3gdawhfn 10 10 in in IN cord-319100-3gdawhfn 10 11 several several JJ cord-319100-3gdawhfn 10 12 commercially commercially RB cord-319100-3gdawhfn 10 13 produced produce VBN cord-319100-3gdawhfn 10 14 transport transport NN cord-319100-3gdawhfn 10 15 media medium NNS cord-319100-3gdawhfn 10 16 and and CC cord-319100-3gdawhfn 10 17 an an DT cord-319100-3gdawhfn 10 18 in in IN cord-319100-3gdawhfn 10 19 - - HYPH cord-319100-3gdawhfn 10 20 house house NN cord-319100-3gdawhfn 10 21 solution solution NN cord-319100-3gdawhfn 10 22 . . . cord-319100-3gdawhfn 11 1 Coronavirus Coronavirus NNP cord-319100-3gdawhfn 11 2 RNA RNA NNP cord-319100-3gdawhfn 11 3 was be VBD cord-319100-3gdawhfn 11 4 rapidly rapidly RB cord-319100-3gdawhfn 11 5 destroyed destroy VBN cord-319100-3gdawhfn 11 6 in in IN cord-319100-3gdawhfn 11 7 the the DT cord-319100-3gdawhfn 11 8 commercial commercial JJ cord-319100-3gdawhfn 11 9 transport transport NN cord-319100-3gdawhfn 11 10 media medium NNS cord-319100-3gdawhfn 11 11 though though IN cord-319100-3gdawhfn 11 12 the the DT cord-319100-3gdawhfn 11 13 deleterious deleterious JJ cord-319100-3gdawhfn 11 14 effects effect NNS cord-319100-3gdawhfn 11 15 on on IN cord-319100-3gdawhfn 11 16 intact intact JJ cord-319100-3gdawhfn 11 17 virus virus NN cord-319100-3gdawhfn 11 18 were be VBD cord-319100-3gdawhfn 11 19 limited limit VBN cord-319100-3gdawhfn 11 20 . . . cord-319100-3gdawhfn 12 1 Similar similar JJ cord-319100-3gdawhfn 12 2 results result NNS cord-319100-3gdawhfn 12 3 were be VBD cord-319100-3gdawhfn 12 4 obtained obtain VBN cord-319100-3gdawhfn 12 5 for for IN cord-319100-3gdawhfn 12 6 a a DT cord-319100-3gdawhfn 12 7 Type type NN cord-319100-3gdawhfn 12 8 A a DT cord-319100-3gdawhfn 12 9 influenza influenza NN cord-319100-3gdawhfn 12 10 virus virus NN cord-319100-3gdawhfn 12 11 . . . cord-319100-3gdawhfn 13 1 There there EX cord-319100-3gdawhfn 13 2 was be VBD cord-319100-3gdawhfn 13 3 reduced reduce VBN cord-319100-3gdawhfn 13 4 detection detection NN cord-319100-3gdawhfn 13 5 of of IN cord-319100-3gdawhfn 13 6 both both DT cord-319100-3gdawhfn 13 7 virus virus NN cord-319100-3gdawhfn 13 8 and and CC cord-319100-3gdawhfn 13 9 nucleic nucleic JJ cord-319100-3gdawhfn 13 10 acid acid NN cord-319100-3gdawhfn 13 11 when when WRB cord-319100-3gdawhfn 13 12 a a DT cord-319100-3gdawhfn 13 13 herpesvirus herpesvirus NN cord-319100-3gdawhfn 13 14 sample sample NN cord-319100-3gdawhfn 13 15 and and CC cord-319100-3gdawhfn 13 16 purified purify VBN cord-319100-3gdawhfn 13 17 DNA DNA NNP cord-319100-3gdawhfn 13 18 were be VBD cord-319100-3gdawhfn 13 19 tested test VBN cord-319100-3gdawhfn 13 20 . . . cord-319100-3gdawhfn 14 1 Collectively collectively RB cord-319100-3gdawhfn 14 2 these these DT cord-319100-3gdawhfn 14 3 data datum NNS cord-319100-3gdawhfn 14 4 showed show VBD cord-319100-3gdawhfn 14 5 that that IN cord-319100-3gdawhfn 14 6 the the DT cord-319100-3gdawhfn 14 7 commercial commercial JJ cord-319100-3gdawhfn 14 8 viral viral JJ cord-319100-3gdawhfn 14 9 transport transport NN cord-319100-3gdawhfn 14 10 media medium NNS cord-319100-3gdawhfn 14 11 contained contain VBN cord-319100-3gdawhfn 14 12 nucleases nuclease NNS cord-319100-3gdawhfn 14 13 or or CC cord-319100-3gdawhfn 14 14 similar similar JJ cord-319100-3gdawhfn 14 15 substances substance NNS cord-319100-3gdawhfn 14 16 and and CC cord-319100-3gdawhfn 14 17 may may MD cord-319100-3gdawhfn 14 18 seriously seriously RB cord-319100-3gdawhfn 14 19 compromise compromise VB cord-319100-3gdawhfn 14 20 diagnostic diagnostic JJ cord-319100-3gdawhfn 14 21 and and CC cord-319100-3gdawhfn 14 22 epidemiological epidemiological JJ cord-319100-3gdawhfn 14 23 investigations investigation NNS cord-319100-3gdawhfn 14 24 . . . cord-319100-3gdawhfn 15 1 Recommendations recommendation NNS cord-319100-3gdawhfn 15 2 to to TO cord-319100-3gdawhfn 15 3 include include VB cord-319100-3gdawhfn 15 4 foetal foetal JJ cord-319100-3gdawhfn 15 5 bovine bovine JJ cord-319100-3gdawhfn 15 6 serum serum NN cord-319100-3gdawhfn 15 7 as as IN cord-319100-3gdawhfn 15 8 a a DT cord-319100-3gdawhfn 15 9 source source NN cord-319100-3gdawhfn 15 10 of of IN cord-319100-3gdawhfn 15 11 protein protein NN cord-319100-3gdawhfn 15 12 to to TO cord-319100-3gdawhfn 15 13 enhance enhance VB cord-319100-3gdawhfn 15 14 the the DT cord-319100-3gdawhfn 15 15 stabilising stabilise VBG cord-319100-3gdawhfn 15 16 properties property NNS cord-319100-3gdawhfn 15 17 of of IN cord-319100-3gdawhfn 15 18 viral viral JJ cord-319100-3gdawhfn 15 19 transport transport NN cord-319100-3gdawhfn 15 20 media medium NNS cord-319100-3gdawhfn 15 21 are be VBP cord-319100-3gdawhfn 15 22 contraindicated contraindicated JJ cord-319100-3gdawhfn 15 23 . . . cord-319100-3gdawhfn 16 1 Almost almost RB cord-319100-3gdawhfn 16 2 all all DT cord-319100-3gdawhfn 16 3 commercial commercial JJ cord-319100-3gdawhfn 16 4 batches batch NNS cord-319100-3gdawhfn 16 5 of of IN cord-319100-3gdawhfn 16 6 foetal foetal JJ cord-319100-3gdawhfn 16 7 bovine bovine JJ cord-319100-3gdawhfn 16 8 serum serum NN cord-319100-3gdawhfn 16 9 contain contain VBP cord-319100-3gdawhfn 16 10 pestiviruses pestiviruse NNS cord-319100-3gdawhfn 16 11 and and CC cord-319100-3gdawhfn 16 12 at at IN cord-319100-3gdawhfn 16 13 times time NNS cord-319100-3gdawhfn 16 14 other other JJ cord-319100-3gdawhfn 16 15 bovine bovine JJ cord-319100-3gdawhfn 16 16 viruses virus NNS cord-319100-3gdawhfn 16 17 . . . cord-319100-3gdawhfn 17 1 In in IN cord-319100-3gdawhfn 17 2 addition addition NN cord-319100-3gdawhfn 17 3 to to IN cord-319100-3gdawhfn 17 4 the the DT cord-319100-3gdawhfn 17 5 potential potential NN cord-319100-3gdawhfn 17 6 for for IN cord-319100-3gdawhfn 17 7 there there EX cord-319100-3gdawhfn 17 8 to to TO cord-319100-3gdawhfn 17 9 be be VB cord-319100-3gdawhfn 17 10 nucleases nuclease NNS cord-319100-3gdawhfn 17 11 in in IN cord-319100-3gdawhfn 17 12 the the DT cord-319100-3gdawhfn 17 13 transport transport NN cord-319100-3gdawhfn 17 14 medium medium NN cord-319100-3gdawhfn 17 15 , , , cord-319100-3gdawhfn 17 16 the the DT cord-319100-3gdawhfn 17 17 presence presence NN cord-319100-3gdawhfn 17 18 of of IN cord-319100-3gdawhfn 17 19 these these DT cord-319100-3gdawhfn 17 20 viruses virus NNS cord-319100-3gdawhfn 17 21 and and CC cord-319100-3gdawhfn 17 22 other other JJ cord-319100-3gdawhfn 17 23 extraneous extraneous JJ cord-319100-3gdawhfn 17 24 nucleic nucleic JJ cord-319100-3gdawhfn 17 25 acid acid NN cord-319100-3gdawhfn 17 26 in in IN cord-319100-3gdawhfn 17 27 samples sample NNS cord-319100-3gdawhfn 17 28 may may MD cord-319100-3gdawhfn 17 29 compromise compromise VB cord-319100-3gdawhfn 17 30 the the DT cord-319100-3gdawhfn 17 31 interpretation interpretation NN cord-319100-3gdawhfn 17 32 of of IN cord-319100-3gdawhfn 17 33 sequence sequence NN cord-319100-3gdawhfn 17 34 data datum NNS cord-319100-3gdawhfn 17 35 . . . cord-319100-3gdawhfn 18 1 The the DT cord-319100-3gdawhfn 18 2 inclusion inclusion NN cord-319100-3gdawhfn 18 3 of of IN cord-319100-3gdawhfn 18 4 foetal foetal JJ cord-319100-3gdawhfn 18 5 bovine bovine JJ cord-319100-3gdawhfn 18 6 serum serum NN cord-319100-3gdawhfn 18 7 presents present VBZ cord-319100-3gdawhfn 18 8 a a DT cord-319100-3gdawhfn 18 9 biosecurity biosecurity NN cord-319100-3gdawhfn 18 10 risk risk NN cord-319100-3gdawhfn 18 11 for for IN cord-319100-3gdawhfn 18 12 the the DT cord-319100-3gdawhfn 18 13 movement movement NN cord-319100-3gdawhfn 18 14 of of IN cord-319100-3gdawhfn 18 15 animal animal NN cord-319100-3gdawhfn 18 16 pathogens pathogen NNS cord-319100-3gdawhfn 18 17 and and CC cord-319100-3gdawhfn 18 18 renders render VBZ cord-319100-3gdawhfn 18 19 these these DT cord-319100-3gdawhfn 18 20 transport transport NN cord-319100-3gdawhfn 18 21 media medium NNS cord-319100-3gdawhfn 18 22 unsuitable unsuitable JJ cord-319100-3gdawhfn 18 23 for for IN cord-319100-3gdawhfn 18 24 animal animal NN cord-319100-3gdawhfn 18 25 disease disease NN cord-319100-3gdawhfn 18 26 diagnostic diagnostic JJ cord-319100-3gdawhfn 18 27 applications application NNS cord-319100-3gdawhfn 18 28 . . . cord-319100-3gdawhfn 19 1 While while IN cord-319100-3gdawhfn 19 2 these these DT cord-319100-3gdawhfn 19 3 transport transport NN cord-319100-3gdawhfn 19 4 media medium NNS cord-319100-3gdawhfn 19 5 may may MD cord-319100-3gdawhfn 19 6 be be VB cord-319100-3gdawhfn 19 7 suitable suitable JJ cord-319100-3gdawhfn 19 8 for for IN cord-319100-3gdawhfn 19 9 virus virus NN cord-319100-3gdawhfn 19 10 culture culture NN cord-319100-3gdawhfn 19 11 purposes purpose NNS cord-319100-3gdawhfn 19 12 , , , cord-319100-3gdawhfn 19 13 there there EX cord-319100-3gdawhfn 19 14 could could MD cord-319100-3gdawhfn 19 15 be be VB cord-319100-3gdawhfn 19 16 misleading misleading JJ cord-319100-3gdawhfn 19 17 results result NNS cord-319100-3gdawhfn 19 18 if if IN cord-319100-3gdawhfn 19 19 used use VBN cord-319100-3gdawhfn 19 20 for for IN cord-319100-3gdawhfn 19 21 nucleic nucleic JJ cord-319100-3gdawhfn 19 22 acid acid NN cord-319100-3gdawhfn 19 23 - - HYPH cord-319100-3gdawhfn 19 24 based base VBN cord-319100-3gdawhfn 19 25 tests test NNS cord-319100-3gdawhfn 19 26 . . . cord-319100-3gdawhfn 20 1 Therefore therefore RB cord-319100-3gdawhfn 20 2 , , , cord-319100-3gdawhfn 20 3 these these DT cord-319100-3gdawhfn 20 4 products product NNS cord-319100-3gdawhfn 20 5 should should MD cord-319100-3gdawhfn 20 6 be be VB cord-319100-3gdawhfn 20 7 evaluated evaluate VBN cord-319100-3gdawhfn 20 8 to to TO cord-319100-3gdawhfn 20 9 ensure ensure VB cord-319100-3gdawhfn 20 10 fitness fitness NN cord-319100-3gdawhfn 20 11 for for IN cord-319100-3gdawhfn 20 12 purpose purpose NN cord-319100-3gdawhfn 20 13 . . . cord-319100-3gdawhfn 21 1 For for IN cord-319100-3gdawhfn 21 2 several several JJ cord-319100-3gdawhfn 21 3 decades decade NNS cord-319100-3gdawhfn 21 4 a a DT cord-319100-3gdawhfn 21 5 variety variety NN cord-319100-3gdawhfn 21 6 of of IN cord-319100-3gdawhfn 21 7 medium medium JJ cord-319100-3gdawhfn 21 8 solutions solution NNS cord-319100-3gdawhfn 21 9 have have VBP cord-319100-3gdawhfn 21 10 been be VBN cord-319100-3gdawhfn 21 11 recommended recommend VBN cord-319100-3gdawhfn 21 12 to to TO cord-319100-3gdawhfn 21 13 stabilise stabilise VB cord-319100-3gdawhfn 21 14 specimens specimen NNS cord-319100-3gdawhfn 21 15 for for IN cord-319100-3gdawhfn 21 16 the the DT cord-319100-3gdawhfn 21 17 detection detection NN cord-319100-3gdawhfn 21 18 of of IN cord-319100-3gdawhfn 21 19 bacteria bacteria NNS cord-319100-3gdawhfn 21 20 and and CC cord-319100-3gdawhfn 21 21 viruses virus NNS cord-319100-3gdawhfn 21 22 , , , cord-319100-3gdawhfn 21 23 particularly particularly RB cord-319100-3gdawhfn 21 24 during during IN cord-319100-3gdawhfn 21 25 diagnostic diagnostic JJ cord-319100-3gdawhfn 21 26 investigations investigation NNS cord-319100-3gdawhfn 21 27 . . . cord-319100-3gdawhfn 22 1 These these DT cord-319100-3gdawhfn 22 2 have have VBP cord-319100-3gdawhfn 22 3 usually usually RB cord-319100-3gdawhfn 22 4 been be VBN cord-319100-3gdawhfn 22 5 based base VBN cord-319100-3gdawhfn 22 6 on on IN cord-319100-3gdawhfn 22 7 balanced balanced JJ cord-319100-3gdawhfn 22 8 salt salt NN cord-319100-3gdawhfn 22 9 or or CC cord-319100-3gdawhfn 22 10 saline saline NN cord-319100-3gdawhfn 22 11 solutions solution NNS cord-319100-3gdawhfn 22 12 with with IN cord-319100-3gdawhfn 22 13 a a DT cord-319100-3gdawhfn 22 14 buffering buffering NN cord-319100-3gdawhfn 22 15 capacity capacity NN cord-319100-3gdawhfn 22 16 to to TO cord-319100-3gdawhfn 22 17 maintain maintain VB cord-319100-3gdawhfn 22 18 a a DT cord-319100-3gdawhfn 22 19 " " `` cord-319100-3gdawhfn 22 20 near near IN cord-319100-3gdawhfn 22 21 - - HYPH cord-319100-3gdawhfn 22 22 neutral neutral JJ cord-319100-3gdawhfn 22 23 " " '' cord-319100-3gdawhfn 22 24 pH. ph. NN cord-319100-3gdawhfn 22 25 To to TO cord-319100-3gdawhfn 22 26 enhance enhance VB cord-319100-3gdawhfn 22 27 the the DT cord-319100-3gdawhfn 22 28 stability stability NN cord-319100-3gdawhfn 22 29 of of IN cord-319100-3gdawhfn 22 30 viruses virus NNS cord-319100-3gdawhfn 22 31 a a DT cord-319100-3gdawhfn 22 32 spectrum spectrum NN cord-319100-3gdawhfn 22 33 of of IN cord-319100-3gdawhfn 22 34 protein protein NN cord-319100-3gdawhfn 22 35 supplements supplement NNS cord-319100-3gdawhfn 22 36 has have VBZ cord-319100-3gdawhfn 22 37 and and CC cord-319100-3gdawhfn 22 38 continues continue VBZ cord-319100-3gdawhfn 22 39 to to TO cord-319100-3gdawhfn 22 40 be be VB cord-319100-3gdawhfn 22 41 recommended recommend VBN cord-319100-3gdawhfn 22 42 ( ( -LRB- cord-319100-3gdawhfn 22 43 1 1 CD cord-319100-3gdawhfn 22 44 , , , cord-319100-3gdawhfn 22 45 2 2 CD cord-319100-3gdawhfn 22 46 , , , cord-319100-3gdawhfn 22 47 3 3 CD cord-319100-3gdawhfn 22 48 , , , cord-319100-3gdawhfn 22 49 4 4 CD cord-319100-3gdawhfn 22 50 ) ) -RRB- cord-319100-3gdawhfn 22 51 . . . cord-319100-3gdawhfn 23 1 While while IN cord-319100-3gdawhfn 23 2 some some DT cord-319100-3gdawhfn 23 3 laboratories laboratory NNS cord-319100-3gdawhfn 23 4 have have VBP cord-319100-3gdawhfn 23 5 prepared prepare VBN cord-319100-3gdawhfn 23 6 viral viral JJ cord-319100-3gdawhfn 23 7 transport transport NN cord-319100-3gdawhfn 23 8 medium medium NN cord-319100-3gdawhfn 23 9 ( ( -LRB- cord-319100-3gdawhfn 23 10 VTM VTM NNP cord-319100-3gdawhfn 23 11 ) ) -RRB- cord-319100-3gdawhfn 23 12 " " `` cord-319100-3gdawhfn 23 13 in in IN cord-319100-3gdawhfn 23 14 house house NN cord-319100-3gdawhfn 23 15 " " '' cord-319100-3gdawhfn 23 16 , , , cord-319100-3gdawhfn 23 17 commercial commercial JJ cord-319100-3gdawhfn 23 18 preparations preparation NNS cord-319100-3gdawhfn 23 19 are be VBP cord-319100-3gdawhfn 23 20 used use VBN cord-319100-3gdawhfn 23 21 extensively extensively RB cord-319100-3gdawhfn 23 22 and and CC cord-319100-3gdawhfn 23 23 are be VBP cord-319100-3gdawhfn 23 24 often often RB cord-319100-3gdawhfn 23 25 supplied supply VBN cord-319100-3gdawhfn 23 26 as as IN cord-319100-3gdawhfn 23 27 part part NN cord-319100-3gdawhfn 23 28 of of IN cord-319100-3gdawhfn 23 29 a a DT cord-319100-3gdawhfn 23 30 sample sample NN cord-319100-3gdawhfn 23 31 collection collection NN cord-319100-3gdawhfn 23 32 kit kit NN cord-319100-3gdawhfn 23 33 with with IN cord-319100-3gdawhfn 23 34 sterile sterile JJ cord-319100-3gdawhfn 23 35 swabs swab NNS cord-319100-3gdawhfn 23 36 . . . cord-319100-3gdawhfn 24 1 Testing testing NN cord-319100-3gdawhfn 24 2 of of IN cord-319100-3gdawhfn 24 3 samples sample NNS cord-319100-3gdawhfn 24 4 by by IN cord-319100-3gdawhfn 24 5 cultural cultural JJ cord-319100-3gdawhfn 24 6 methods method NNS cord-319100-3gdawhfn 24 7 meant mean VBD cord-319100-3gdawhfn 24 8 that that IN cord-319100-3gdawhfn 24 9 the the DT cord-319100-3gdawhfn 24 10 emphasis emphasis NN cord-319100-3gdawhfn 24 11 of of IN cord-319100-3gdawhfn 24 12 studies study NNS cord-319100-3gdawhfn 24 13 for for IN cord-319100-3gdawhfn 24 14 the the DT cord-319100-3gdawhfn 24 15 evaluation evaluation NN cord-319100-3gdawhfn 24 16 of of IN cord-319100-3gdawhfn 24 17 these these DT cord-319100-3gdawhfn 24 18 products product NNS cord-319100-3gdawhfn 24 19 originally originally RB cord-319100-3gdawhfn 24 20 focussed focusse VBN cord-319100-3gdawhfn 24 21 on on IN cord-319100-3gdawhfn 24 22 the the DT cord-319100-3gdawhfn 24 23 capacity capacity NN cord-319100-3gdawhfn 24 24 of of IN cord-319100-3gdawhfn 24 25 a a DT cord-319100-3gdawhfn 24 26 preparation preparation NN cord-319100-3gdawhfn 24 27 to to TO cord-319100-3gdawhfn 24 28 maintain maintain VB cord-319100-3gdawhfn 24 29 the the DT cord-319100-3gdawhfn 24 30 infectivity infectivity NN cord-319100-3gdawhfn 24 31 of of IN cord-319100-3gdawhfn 24 32 viruses virus NNS cord-319100-3gdawhfn 24 33 at at IN cord-319100-3gdawhfn 24 34 different different JJ cord-319100-3gdawhfn 24 35 temperatures temperature NNS cord-319100-3gdawhfn 24 36 while while IN cord-319100-3gdawhfn 24 37 being be VBG cord-319100-3gdawhfn 24 38 held hold VBN cord-319100-3gdawhfn 24 39 prior prior RB cord-319100-3gdawhfn 24 40 to to IN cord-319100-3gdawhfn 24 41 and and CC cord-319100-3gdawhfn 24 42 during during IN cord-319100-3gdawhfn 24 43 transport transport NN cord-319100-3gdawhfn 24 44 and and CC cord-319100-3gdawhfn 24 45 while while IN cord-319100-3gdawhfn 24 46 being be VBG cord-319100-3gdawhfn 24 47 stored store VBN cord-319100-3gdawhfn 24 48 at at IN cord-319100-3gdawhfn 24 49 the the DT cord-319100-3gdawhfn 24 50 laboratory laboratory NN cord-319100-3gdawhfn 24 51 ( ( -LRB- cord-319100-3gdawhfn 24 52 5 5 CD cord-319100-3gdawhfn 24 53 , , , cord-319100-3gdawhfn 24 54 6 6 CD cord-319100-3gdawhfn 24 55 ) ) -RRB- cord-319100-3gdawhfn 24 56 . . . cord-319100-3gdawhfn 25 1 With with IN cord-319100-3gdawhfn 25 2 the the DT cord-319100-3gdawhfn 25 3 widespread widespread JJ cord-319100-3gdawhfn 25 4 introduction introduction NN cord-319100-3gdawhfn 25 5 of of IN cord-319100-3gdawhfn 25 6 molecular molecular JJ cord-319100-3gdawhfn 25 7 based base VBN cord-319100-3gdawhfn 25 8 diagnostic diagnostic JJ cord-319100-3gdawhfn 25 9 assays assay NNS cord-319100-3gdawhfn 25 10 , , , cord-319100-3gdawhfn 25 11 especially especially RB cord-319100-3gdawhfn 25 12 real real JJ cord-319100-3gdawhfn 25 13 time time NN cord-319100-3gdawhfn 25 14 PCR PCR NNP cord-319100-3gdawhfn 25 15 ( ( -LRB- cord-319100-3gdawhfn 25 16 qPCR qPCR NNP cord-319100-3gdawhfn 25 17 ) ) -RRB- cord-319100-3gdawhfn 25 18 , , , cord-319100-3gdawhfn 25 19 studies study NNS cord-319100-3gdawhfn 25 20 have have VBP cord-319100-3gdawhfn 25 21 been be VBN cord-319100-3gdawhfn 25 22 undertaken undertake VBN cord-319100-3gdawhfn 25 23 to to TO cord-319100-3gdawhfn 25 24 evaluate evaluate VB cord-319100-3gdawhfn 25 25 the the DT cord-319100-3gdawhfn 25 26 stability stability NN cord-319100-3gdawhfn 25 27 of of IN cord-319100-3gdawhfn 25 28 viruses virus NNS cord-319100-3gdawhfn 25 29 in in IN cord-319100-3gdawhfn 25 30 VTMs vtm NNS cord-319100-3gdawhfn 25 31 , , , cord-319100-3gdawhfn 25 32 particularly particularly RB cord-319100-3gdawhfn 25 33 in in IN cord-319100-3gdawhfn 25 34 commercially commercially RB cord-319100-3gdawhfn 25 35 prepared prepared JJ cord-319100-3gdawhfn 25 36 products product NNS cord-319100-3gdawhfn 25 37 , , , cord-319100-3gdawhfn 25 38 while while IN cord-319100-3gdawhfn 25 39 being be VBG cord-319100-3gdawhfn 25 40 held hold VBN cord-319100-3gdawhfn 25 41 at at IN cord-319100-3gdawhfn 25 42 a a DT cord-319100-3gdawhfn 25 43 range range NN cord-319100-3gdawhfn 25 44 of of IN cord-319100-3gdawhfn 25 45 temperatures temperature NNS cord-319100-3gdawhfn 25 46 ( ( -LRB- cord-319100-3gdawhfn 25 47 7 7 CD cord-319100-3gdawhfn 25 48 , , , cord-319100-3gdawhfn 25 49 8 8 CD cord-319100-3gdawhfn 25 50 , , , cord-319100-3gdawhfn 25 51 9 9 CD cord-319100-3gdawhfn 25 52 ) ) -RRB- cord-319100-3gdawhfn 25 53 . . . cord-319100-3gdawhfn 26 1 However however RB cord-319100-3gdawhfn 26 2 , , , cord-319100-3gdawhfn 26 3 while while IN cord-319100-3gdawhfn 26 4 thermal thermal JJ cord-319100-3gdawhfn 26 5 stability stability NN cord-319100-3gdawhfn 26 6 has have VBZ cord-319100-3gdawhfn 26 7 been be VBN cord-319100-3gdawhfn 26 8 considered consider VBN cord-319100-3gdawhfn 26 9 , , , cord-319100-3gdawhfn 26 10 generally generally RB cord-319100-3gdawhfn 26 11 little little JJ cord-319100-3gdawhfn 26 12 attention attention NN cord-319100-3gdawhfn 26 13 has have VBZ cord-319100-3gdawhfn 26 14 been be VBN cord-319100-3gdawhfn 26 15 given give VBN cord-319100-3gdawhfn 26 16 to to IN cord-319100-3gdawhfn 26 17 other other JJ cord-319100-3gdawhfn 26 18 characteristics characteristic NNS cord-319100-3gdawhfn 26 19 of of IN cord-319100-3gdawhfn 26 20 the the DT cord-319100-3gdawhfn 26 21 VTM VTM NNP cord-319100-3gdawhfn 26 22 or or CC cord-319100-3gdawhfn 26 23 the the DT cord-319100-3gdawhfn 26 24 potential potential JJ cord-319100-3gdawhfn 26 25 impact impact NN cord-319100-3gdawhfn 26 26 of of IN cord-319100-3gdawhfn 26 27 endogenous endogenous JJ cord-319100-3gdawhfn 26 28 components component NNS cord-319100-3gdawhfn 26 29 . . . cord-319100-3gdawhfn 27 1 One one CD cord-319100-3gdawhfn 27 2 commercially commercially RB cord-319100-3gdawhfn 27 3 available available JJ cord-319100-3gdawhfn 27 4 product product NN cord-319100-3gdawhfn 27 5 is be VBZ cord-319100-3gdawhfn 27 6 specifically specifically RB cord-319100-3gdawhfn 27 7 designed design VBN cord-319100-3gdawhfn 27 8 to to TO cord-319100-3gdawhfn 27 9 inactivate inactivate VB cord-319100-3gdawhfn 27 10 viruses virus NNS cord-319100-3gdawhfn 27 11 and and CC cord-319100-3gdawhfn 27 12 bacteria bacteria NNS cord-319100-3gdawhfn 27 13 and and CC cord-319100-3gdawhfn 27 14 contains contain VBZ cord-319100-3gdawhfn 27 15 components component NNS cord-319100-3gdawhfn 27 16 to to TO cord-319100-3gdawhfn 27 17 inhibit inhibit VB cord-319100-3gdawhfn 27 18 the the DT cord-319100-3gdawhfn 27 19 activity activity NN cord-319100-3gdawhfn 27 20 of of IN cord-319100-3gdawhfn 27 21 nucleases nuclease NNS cord-319100-3gdawhfn 27 22 that that WDT cord-319100-3gdawhfn 27 23 may may MD cord-319100-3gdawhfn 27 24 be be VB cord-319100-3gdawhfn 27 25 present present JJ cord-319100-3gdawhfn 27 26 in in IN cord-319100-3gdawhfn 27 27 the the DT cord-319100-3gdawhfn 27 28 sample sample NN cord-319100-3gdawhfn 27 29 ( ( -LRB- cord-319100-3gdawhfn 27 30 10 10 CD cord-319100-3gdawhfn 27 31 , , , cord-319100-3gdawhfn 27 32 11 11 CD cord-319100-3gdawhfn 27 33 ) ) -RRB- cord-319100-3gdawhfn 27 34 . . . cord-319100-3gdawhfn 28 1 There there EX cord-319100-3gdawhfn 28 2 are be VBP cord-319100-3gdawhfn 28 3 some some DT cord-319100-3gdawhfn 28 4 other other JJ cord-319100-3gdawhfn 28 5 products product NNS cord-319100-3gdawhfn 28 6 that that WDT cord-319100-3gdawhfn 28 7 are be VBP cord-319100-3gdawhfn 28 8 recommended recommend VBN cord-319100-3gdawhfn 28 9 for for IN cord-319100-3gdawhfn 28 10 use use NN cord-319100-3gdawhfn 28 11 in in IN cord-319100-3gdawhfn 28 12 molecular molecular JJ cord-319100-3gdawhfn 28 13 detection detection NN cord-319100-3gdawhfn 28 14 assays assay NNS cord-319100-3gdawhfn 28 15 but but CC cord-319100-3gdawhfn 28 16 the the DT cord-319100-3gdawhfn 28 17 manufacturers manufacturer NNS cord-319100-3gdawhfn 28 18 provide provide VBP cord-319100-3gdawhfn 28 19 no no DT cord-319100-3gdawhfn 28 20 comment comment NN cord-319100-3gdawhfn 28 21 that that IN cord-319100-3gdawhfn 28 22 these these DT cord-319100-3gdawhfn 28 23 products product NNS cord-319100-3gdawhfn 28 24 are be VBP cord-319100-3gdawhfn 28 25 unlikely unlikely JJ cord-319100-3gdawhfn 28 26 to to TO cord-319100-3gdawhfn 28 27 be be VB cord-319100-3gdawhfn 28 28 suitable suitable JJ cord-319100-3gdawhfn 28 29 for for IN cord-319100-3gdawhfn 28 30 samples sample NNS cord-319100-3gdawhfn 28 31 where where WRB cord-319100-3gdawhfn 28 32 virus virus NN cord-319100-3gdawhfn 28 33 culture culture NN cord-319100-3gdawhfn 28 34 will will MD cord-319100-3gdawhfn 28 35 be be VB cord-319100-3gdawhfn 28 36 attempted attempt VBN cord-319100-3gdawhfn 28 37 . . . cord-319100-3gdawhfn 29 1 During during IN cord-319100-3gdawhfn 29 2 large large JJ cord-319100-3gdawhfn 29 3 - - HYPH cord-319100-3gdawhfn 29 4 scale scale NN cord-319100-3gdawhfn 29 5 disease disease NN cord-319100-3gdawhfn 29 6 epidemics epidemic NNS cord-319100-3gdawhfn 29 7 there there EX cord-319100-3gdawhfn 29 8 can can MD cord-319100-3gdawhfn 29 9 be be VB cord-319100-3gdawhfn 29 10 pressure pressure NN cord-319100-3gdawhfn 29 11 placed place VBN cord-319100-3gdawhfn 29 12 on on IN cord-319100-3gdawhfn 29 13 the the DT cord-319100-3gdawhfn 29 14 capacity capacity NN cord-319100-3gdawhfn 29 15 of of IN cord-319100-3gdawhfn 29 16 manufacturers manufacturer NNS cord-319100-3gdawhfn 29 17 to to TO cord-319100-3gdawhfn 29 18 supply supply VB cord-319100-3gdawhfn 29 19 transport transport NN cord-319100-3gdawhfn 29 20 media medium NNS cord-319100-3gdawhfn 29 21 and and CC cord-319100-3gdawhfn 29 22 , , , cord-319100-3gdawhfn 29 23 during during IN cord-319100-3gdawhfn 29 24 a a DT cord-319100-3gdawhfn 29 25 pandemic pandemic NN cord-319100-3gdawhfn 29 26 , , , cord-319100-3gdawhfn 29 27 supply supply NN cord-319100-3gdawhfn 29 28 - - HYPH cord-319100-3gdawhfn 29 29 chain chain NN cord-319100-3gdawhfn 29 30 and and CC cord-319100-3gdawhfn 29 31 manufacturing manufacturing NN cord-319100-3gdawhfn 29 32 pressures pressure NNS cord-319100-3gdawhfn 29 33 can can MD cord-319100-3gdawhfn 29 34 become become VB cord-319100-3gdawhfn 29 35 prohibitive prohibitive JJ cord-319100-3gdawhfn 29 36 . . . cord-319100-3gdawhfn 30 1 During during IN cord-319100-3gdawhfn 30 2 the the DT cord-319100-3gdawhfn 30 3 current current JJ cord-319100-3gdawhfn 30 4 ( ( -LRB- cord-319100-3gdawhfn 30 5 2020 2020 CD cord-319100-3gdawhfn 30 6 ) ) -RRB- cord-319100-3gdawhfn 30 7 SARS SARS NNP cord-319100-3gdawhfn 30 8 - - HYPH cord-319100-3gdawhfn 30 9 Cov-2 Cov-2 NNP cord-319100-3gdawhfn 30 10 pandemic pandemic NN cord-319100-3gdawhfn 30 11 , , , cord-319100-3gdawhfn 30 12 there there EX cord-319100-3gdawhfn 30 13 has have VBZ cord-319100-3gdawhfn 30 14 been be VBN cord-319100-3gdawhfn 30 15 an an DT cord-319100-3gdawhfn 30 16 acute acute JJ cord-319100-3gdawhfn 30 17 shortage shortage NN cord-319100-3gdawhfn 30 18 of of IN cord-319100-3gdawhfn 30 19 VTM VTM NNP cord-319100-3gdawhfn 30 20 in in IN cord-319100-3gdawhfn 30 21 Australia Australia NNP cord-319100-3gdawhfn 30 22 because because IN cord-319100-3gdawhfn 30 23 of of IN cord-319100-3gdawhfn 30 24 a a DT cord-319100-3gdawhfn 30 25 combination combination NN cord-319100-3gdawhfn 30 26 of of IN cord-319100-3gdawhfn 30 27 both both CC cord-319100-3gdawhfn 30 28 local local JJ cord-319100-3gdawhfn 30 29 and and CC cord-319100-3gdawhfn 30 30 international international JJ cord-319100-3gdawhfn 30 31 demand demand NN cord-319100-3gdawhfn 30 32 , , , cord-319100-3gdawhfn 30 33 the the DT cord-319100-3gdawhfn 30 34 lack lack NN cord-319100-3gdawhfn 30 35 of of IN cord-319100-3gdawhfn 30 36 a a DT cord-319100-3gdawhfn 30 37 local local JJ cord-319100-3gdawhfn 30 38 manufacturer manufacturer NN cord-319100-3gdawhfn 30 39 and and CC cord-319100-3gdawhfn 30 40 partly partly RB cord-319100-3gdawhfn 30 41 because because IN cord-319100-3gdawhfn 30 42 of of IN cord-319100-3gdawhfn 30 43 reduced reduce VBN cord-319100-3gdawhfn 30 44 international international JJ cord-319100-3gdawhfn 30 45 airline airline NN cord-319100-3gdawhfn 30 46 flights flight NNS cord-319100-3gdawhfn 30 47 to to IN cord-319100-3gdawhfn 30 48 Australia Australia NNP cord-319100-3gdawhfn 30 49 . . . cord-319100-3gdawhfn 31 1 Consequently consequently RB cord-319100-3gdawhfn 31 2 , , , cord-319100-3gdawhfn 31 3 many many JJ cord-319100-3gdawhfn 31 4 different different JJ cord-319100-3gdawhfn 31 5 VTMs vtm NNS cord-319100-3gdawhfn 31 6 and and CC cord-319100-3gdawhfn 31 7 similar similar JJ cord-319100-3gdawhfn 31 8 solutions solution NNS cord-319100-3gdawhfn 31 9 have have VBP cord-319100-3gdawhfn 31 10 been be VBN cord-319100-3gdawhfn 31 11 used use VBN cord-319100-3gdawhfn 31 12 to to TO cord-319100-3gdawhfn 31 13 meet meet VB cord-319100-3gdawhfn 31 14 the the DT cord-319100-3gdawhfn 31 15 demand demand NN cord-319100-3gdawhfn 31 16 for for IN cord-319100-3gdawhfn 31 17 transport transport NN cord-319100-3gdawhfn 31 18 media medium NNS cord-319100-3gdawhfn 31 19 generated generate VBN cord-319100-3gdawhfn 31 20 by by IN cord-319100-3gdawhfn 31 21 large large JJ cord-319100-3gdawhfn 31 22 scale scale NN cord-319100-3gdawhfn 31 23 diagnostic diagnostic NN cord-319100-3gdawhfn 31 24 and and CC cord-319100-3gdawhfn 31 25 surveillance surveillance NN cord-319100-3gdawhfn 31 26 testing testing NN cord-319100-3gdawhfn 31 27 . . . cord-319100-3gdawhfn 32 1 After after IN cord-319100-3gdawhfn 32 2 becoming become VBG cord-319100-3gdawhfn 32 3 aware aware JJ cord-319100-3gdawhfn 32 4 of of IN cord-319100-3gdawhfn 32 5 concerns concern NNS cord-319100-3gdawhfn 32 6 of of IN cord-319100-3gdawhfn 32 7 variable variable JJ cord-319100-3gdawhfn 32 8 results result NNS cord-319100-3gdawhfn 32 9 in in IN cord-319100-3gdawhfn 32 10 some some DT cord-319100-3gdawhfn 32 11 assays assay NNS cord-319100-3gdawhfn 32 12 , , , cord-319100-3gdawhfn 32 13 we -PRON- PRP cord-319100-3gdawhfn 32 14 initiated initiate VBD cord-319100-3gdawhfn 32 15 a a DT cord-319100-3gdawhfn 32 16 preliminary preliminary JJ cord-319100-3gdawhfn 32 17 study study NN cord-319100-3gdawhfn 32 18 to to TO cord-319100-3gdawhfn 32 19 compare compare VB cord-319100-3gdawhfn 32 20 the the DT cord-319100-3gdawhfn 32 21 stability stability NN cord-319100-3gdawhfn 32 22 of of IN cord-319100-3gdawhfn 32 23 SARS SARS NNP cord-319100-3gdawhfn 32 24 - - HYPH cord-319100-3gdawhfn 32 25 Cov-2 Cov-2 NNP cord-319100-3gdawhfn 32 26 RNA RNA NNP cord-319100-3gdawhfn 32 27 in in IN cord-319100-3gdawhfn 32 28 several several JJ cord-319100-3gdawhfn 32 29 commercially commercially RB cord-319100-3gdawhfn 32 30 available available JJ cord-319100-3gdawhfn 32 31 VTMs vtm NNS cord-319100-3gdawhfn 32 32 and and CC cord-319100-3gdawhfn 32 33 an an DT cord-319100-3gdawhfn 32 34 in in IN cord-319100-3gdawhfn 32 35 - - HYPH cord-319100-3gdawhfn 32 36 house house NN cord-319100-3gdawhfn 32 37 product product NN cord-319100-3gdawhfn 32 38 . . . cord-319100-3gdawhfn 33 1 When when WRB cord-319100-3gdawhfn 33 2 disturbing disturbing JJ cord-319100-3gdawhfn 33 3 results result NNS cord-319100-3gdawhfn 33 4 were be VBD cord-319100-3gdawhfn 33 5 found find VBN cord-319100-3gdawhfn 33 6 in in IN cord-319100-3gdawhfn 33 7 a a DT cord-319100-3gdawhfn 33 8 preliminary preliminary JJ cord-319100-3gdawhfn 33 9 SARS SARS NNP cord-319100-3gdawhfn 33 10 - - HYPH cord-319100-3gdawhfn 33 11 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 33 12 study study NN cord-319100-3gdawhfn 33 13 , , , cord-319100-3gdawhfn 33 14 the the DT cord-319100-3gdawhfn 33 15 investigation investigation NN cord-319100-3gdawhfn 33 16 was be VBD cord-319100-3gdawhfn 33 17 extended extend VBN cord-319100-3gdawhfn 33 18 to to TO cord-319100-3gdawhfn 33 19 examine examine VB cord-319100-3gdawhfn 33 20 whether whether IN cord-319100-3gdawhfn 33 21 there there EX cord-319100-3gdawhfn 33 22 was be VBD cord-319100-3gdawhfn 33 23 also also RB cord-319100-3gdawhfn 33 24 an an DT cord-319100-3gdawhfn 33 25 adverse adverse JJ cord-319100-3gdawhfn 33 26 impact impact NN cord-319100-3gdawhfn 33 27 on on IN cord-319100-3gdawhfn 33 28 qPCR qpcr NN cord-319100-3gdawhfn 33 29 results result NNS cord-319100-3gdawhfn 33 30 for for IN cord-319100-3gdawhfn 33 31 other other JJ cord-319100-3gdawhfn 33 32 viruses virus NNS cord-319100-3gdawhfn 33 33 . . . cord-319100-3gdawhfn 34 1 Also also RB cord-319100-3gdawhfn 34 2 of of IN cord-319100-3gdawhfn 34 3 concern concern NN cord-319100-3gdawhfn 34 4 are be VBP cord-319100-3gdawhfn 34 5 recommendations recommendation NNS cord-319100-3gdawhfn 34 6 ( ( -LRB- cord-319100-3gdawhfn 34 7 3 3 CD cord-319100-3gdawhfn 34 8 , , , cord-319100-3gdawhfn 34 9 4 4 CD cord-319100-3gdawhfn 34 10 ) ) -RRB- cord-319100-3gdawhfn 34 11 to to TO cord-319100-3gdawhfn 34 12 include include VB cord-319100-3gdawhfn 34 13 foetal foetal JJ cord-319100-3gdawhfn 34 14 bovine bovine JJ cord-319100-3gdawhfn 34 15 serum serum NN cord-319100-3gdawhfn 34 16 ( ( -LRB- cord-319100-3gdawhfn 34 17 fbs fbs NNP cord-319100-3gdawhfn 34 18 ) ) -RRB- cord-319100-3gdawhfn 34 19 as as IN cord-319100-3gdawhfn 34 20 a a DT cord-319100-3gdawhfn 34 21 source source NN cord-319100-3gdawhfn 34 22 of of IN cord-319100-3gdawhfn 34 23 protein protein NN cord-319100-3gdawhfn 34 24 to to TO cord-319100-3gdawhfn 34 25 enhance enhance VB cord-319100-3gdawhfn 34 26 the the DT cord-319100-3gdawhfn 34 27 stabilising stabilise VBG cord-319100-3gdawhfn 34 28 properties property NNS cord-319100-3gdawhfn 34 29 of of IN cord-319100-3gdawhfn 34 30 VTMs vtm NNS cord-319100-3gdawhfn 34 31 . . . cord-319100-3gdawhfn 35 1 This this DT cord-319100-3gdawhfn 35 2 report report NN cord-319100-3gdawhfn 35 3 documents document VBZ cord-319100-3gdawhfn 35 4 observations observation NNS cord-319100-3gdawhfn 35 5 of of IN cord-319100-3gdawhfn 35 6 the the DT cord-319100-3gdawhfn 35 7 adverse adverse JJ cord-319100-3gdawhfn 35 8 impact impact NN cord-319100-3gdawhfn 35 9 of of IN cord-319100-3gdawhfn 35 10 certain certain JJ cord-319100-3gdawhfn 35 11 VTMs vtm NNS cord-319100-3gdawhfn 35 12 on on IN cord-319100-3gdawhfn 35 13 real real JJ cord-319100-3gdawhfn 35 14 time time NN cord-319100-3gdawhfn 35 15 reverse reverse NN cord-319100-3gdawhfn 35 16 transcription transcription NN cord-319100-3gdawhfn 35 17 PCR PCR NNP cord-319100-3gdawhfn 35 18 ( ( -LRB- cord-319100-3gdawhfn 35 19 qRT qRT NNP cord-319100-3gdawhfn 35 20 - - HYPH cord-319100-3gdawhfn 35 21 PCR PCR NNP cord-319100-3gdawhfn 35 22 ) ) -RRB- cord-319100-3gdawhfn 35 23 assays assay NNS cord-319100-3gdawhfn 35 24 for for IN cord-319100-3gdawhfn 35 25 the the DT cord-319100-3gdawhfn 35 26 detection detection NN cord-319100-3gdawhfn 35 27 of of IN cord-319100-3gdawhfn 35 28 SARS SARS NNP cord-319100-3gdawhfn 35 29 - - HYPH cord-319100-3gdawhfn 35 30 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 35 31 virus virus NN cord-319100-3gdawhfn 35 32 as as RB cord-319100-3gdawhfn 35 33 well well RB cord-319100-3gdawhfn 35 34 as as IN cord-319100-3gdawhfn 35 35 on on IN cord-319100-3gdawhfn 35 36 a a DT cord-319100-3gdawhfn 35 37 Type type NN cord-319100-3gdawhfn 35 38 A a DT cord-319100-3gdawhfn 35 39 influenza influenza NN cord-319100-3gdawhfn 35 40 virus virus NN cord-319100-3gdawhfn 35 41 and and CC cord-319100-3gdawhfn 35 42 a a DT cord-319100-3gdawhfn 35 43 herpesvirus herpesvirus NN cord-319100-3gdawhfn 35 44 and and CC cord-319100-3gdawhfn 35 45 discuss discuss VB cord-319100-3gdawhfn 35 46 the the DT cord-319100-3gdawhfn 35 47 broader broad JJR cord-319100-3gdawhfn 35 48 implications implication NNS cord-319100-3gdawhfn 35 49 of of IN cord-319100-3gdawhfn 35 50 the the DT cord-319100-3gdawhfn 35 51 inclusion inclusion NN cord-319100-3gdawhfn 35 52 of of IN cord-319100-3gdawhfn 35 53 foetal foetal JJ cord-319100-3gdawhfn 35 54 bovine bovine JJ cord-319100-3gdawhfn 35 55 serum serum NN cord-319100-3gdawhfn 35 56 as as IN cord-319100-3gdawhfn 35 57 a a DT cord-319100-3gdawhfn 35 58 protein protein NN cord-319100-3gdawhfn 35 59 supplement supplement NN cord-319100-3gdawhfn 35 60 to to IN cord-319100-3gdawhfn 35 61 VTMs VTMs NNP cord-319100-3gdawhfn 35 62 . . . cord-319100-3gdawhfn 36 1 During during IN cord-319100-3gdawhfn 36 2 the the DT cord-319100-3gdawhfn 36 3 initial initial JJ cord-319100-3gdawhfn 36 4 investigation investigation NN cord-319100-3gdawhfn 36 5 , , , cord-319100-3gdawhfn 36 6 purified purify VBN cord-319100-3gdawhfn 36 7 RNA RNA NNP cord-319100-3gdawhfn 36 8 from from IN cord-319100-3gdawhfn 36 9 an an DT cord-319100-3gdawhfn 36 10 Australian australian JJ cord-319100-3gdawhfn 36 11 isolate isolate NN cord-319100-3gdawhfn 36 12 ( ( -LRB- cord-319100-3gdawhfn 36 13 WMD WMD NNP cord-319100-3gdawhfn 36 14 DC1 DC1 NNP cord-319100-3gdawhfn 36 15 ) ) -RRB- cord-319100-3gdawhfn 36 16 of of IN cord-319100-3gdawhfn 36 17 SARS SARS NNP cord-319100-3gdawhfn 36 18 - - HYPH cord-319100-3gdawhfn 36 19 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 36 20 was be VBD cord-319100-3gdawhfn 36 21 supplied supply VBN cord-319100-3gdawhfn 36 22 to to IN cord-319100-3gdawhfn 36 23 the the DT cord-319100-3gdawhfn 36 24 Elizabeth Elizabeth NNP cord-319100-3gdawhfn 36 25 Macarthur Macarthur NNP cord-319100-3gdawhfn 36 26 Agriculture Agriculture NNP cord-319100-3gdawhfn 36 27 Institute Institute NNP cord-319100-3gdawhfn 36 28 ( ( -LRB- cord-319100-3gdawhfn 36 29 EMAI EMAI NNP cord-319100-3gdawhfn 36 30 ) ) -RRB- cord-319100-3gdawhfn 36 31 by by IN cord-319100-3gdawhfn 36 32 the the DT cord-319100-3gdawhfn 36 33 Institute Institute NNP cord-319100-3gdawhfn 36 34 of of IN cord-319100-3gdawhfn 36 35 Clinical Clinical NNP cord-319100-3gdawhfn 36 36 Pathology Pathology NNP cord-319100-3gdawhfn 36 37 and and CC cord-319100-3gdawhfn 36 38 Medical Medical NNP cord-319100-3gdawhfn 36 39 Research Research NNP cord-319100-3gdawhfn 36 40 ( ( -LRB- cord-319100-3gdawhfn 36 41 ICPMR ICPMR NNP cord-319100-3gdawhfn 36 42 ) ) -RRB- cord-319100-3gdawhfn 36 43 , , , cord-319100-3gdawhfn 36 44 Westmead Westmead NNP cord-319100-3gdawhfn 36 45 , , , cord-319100-3gdawhfn 36 46 New New NNP cord-319100-3gdawhfn 36 47 South South NNP cord-319100-3gdawhfn 36 48 Wales Wales NNP cord-319100-3gdawhfn 36 49 ( ( -LRB- cord-319100-3gdawhfn 36 50 NSW NSW NNP cord-319100-3gdawhfn 36 51 ) ) -RRB- cord-319100-3gdawhfn 36 52 . . . cord-319100-3gdawhfn 37 1 A a DT cord-319100-3gdawhfn 37 2 1/1000 1/1000 CD cord-319100-3gdawhfn 37 3 dilution dilution NN cord-319100-3gdawhfn 37 4 of of IN cord-319100-3gdawhfn 37 5 this this DT cord-319100-3gdawhfn 37 6 RNA RNA NNP cord-319100-3gdawhfn 37 7 was be VBD cord-319100-3gdawhfn 37 8 made make VBN cord-319100-3gdawhfn 37 9 in in IN cord-319100-3gdawhfn 37 10 tRNA tRNA NNP cord-319100-3gdawhfn 37 11 ( ( -LRB- cord-319100-3gdawhfn 37 12 Sigma Sigma NNP cord-319100-3gdawhfn 37 13 , , , cord-319100-3gdawhfn 37 14 40ng 40ng JJ cord-319100-3gdawhfn 37 15 / / SYM cord-319100-3gdawhfn 37 16 ul ul NN cord-319100-3gdawhfn 37 17 ) ) -RRB- cord-319100-3gdawhfn 37 18 to to TO cord-319100-3gdawhfn 37 19 use use VB cord-319100-3gdawhfn 37 20 as as IN cord-319100-3gdawhfn 37 21 a a DT cord-319100-3gdawhfn 37 22 reference reference NN cord-319100-3gdawhfn 37 23 preparation preparation NN cord-319100-3gdawhfn 37 24 ( ( -LRB- cord-319100-3gdawhfn 37 25 designated designate VBN cord-319100-3gdawhfn 37 26 R7215 R7215 NNP cord-319100-3gdawhfn 37 27 ) ) -RRB- cord-319100-3gdawhfn 37 28 and and CC cord-319100-3gdawhfn 37 29 stored store VBD cord-319100-3gdawhfn 37 30 frozen frozen JJ cord-319100-3gdawhfn 37 31 at at IN cord-319100-3gdawhfn 37 32 approximately approximately RB cord-319100-3gdawhfn 37 33 -80 -80 NNP cord-319100-3gdawhfn 38 1 o o UH cord-319100-3gdawhfn 38 2 C. c. NN cord-319100-3gdawhfn 39 1 Subsequently subsequently RB cord-319100-3gdawhfn 39 2 , , , cord-319100-3gdawhfn 39 3 to to TO cord-319100-3gdawhfn 39 4 include include VB cord-319100-3gdawhfn 39 5 a a DT cord-319100-3gdawhfn 39 6 sample sample NN cord-319100-3gdawhfn 39 7 which which WDT cord-319100-3gdawhfn 39 8 was be VBD cord-319100-3gdawhfn 39 9 expected expect VBN cord-319100-3gdawhfn 39 10 to to TO cord-319100-3gdawhfn 39 11 contain contain VB cord-319100-3gdawhfn 39 12 a a DT cord-319100-3gdawhfn 39 13 high high JJ cord-319100-3gdawhfn 39 14 titre titre NN cord-319100-3gdawhfn 39 15 of of IN cord-319100-3gdawhfn 39 16 intact intact JJ cord-319100-3gdawhfn 39 17 , , , cord-319100-3gdawhfn 39 18 likely likely JJ cord-319100-3gdawhfn 39 19 infectious infectious JJ cord-319100-3gdawhfn 39 20 virus virus NN cord-319100-3gdawhfn 39 21 , , , cord-319100-3gdawhfn 39 22 a a DT cord-319100-3gdawhfn 39 23 sample sample NN cord-319100-3gdawhfn 39 24 from from IN cord-319100-3gdawhfn 39 25 a a DT cord-319100-3gdawhfn 39 26 recently recently RB cord-319100-3gdawhfn 39 27 diagnosed diagnose VBN cord-319100-3gdawhfn 39 28 patient patient NN cord-319100-3gdawhfn 39 29 was be VBD cord-319100-3gdawhfn 39 30 selected select VBN cord-319100-3gdawhfn 39 31 ( ( -LRB- cord-319100-3gdawhfn 39 32 P66 P66 NNP cord-319100-3gdawhfn 39 33 ) ) -RRB- cord-319100-3gdawhfn 39 34 . . . cord-319100-3gdawhfn 40 1 This this DT cord-319100-3gdawhfn 40 2 sample sample NN cord-319100-3gdawhfn 40 3 had have VBD cord-319100-3gdawhfn 40 4 also also RB cord-319100-3gdawhfn 40 5 been be VBN cord-319100-3gdawhfn 40 6 stored store VBN cord-319100-3gdawhfn 40 7 frozen frozen JJ cord-319100-3gdawhfn 40 8 at at IN cord-319100-3gdawhfn 40 9 approximately approximately RB cord-319100-3gdawhfn 40 10 -80 -80 NNP cord-319100-3gdawhfn 40 11 o o NN cord-319100-3gdawhfn 40 12 C c NN cord-319100-3gdawhfn 40 13 soon soon RB cord-319100-3gdawhfn 40 14 after after IN cord-319100-3gdawhfn 40 15 it -PRON- PRP cord-319100-3gdawhfn 40 16 had have VBD cord-319100-3gdawhfn 40 17 been be VBN cord-319100-3gdawhfn 40 18 tested test VBN cord-319100-3gdawhfn 40 19 a a DT cord-319100-3gdawhfn 40 20 few few JJ cord-319100-3gdawhfn 40 21 days day NNS cord-319100-3gdawhfn 40 22 earlier early RBR cord-319100-3gdawhfn 40 23 . . . cord-319100-3gdawhfn 41 1 Dilutions dilution NNS cord-319100-3gdawhfn 41 2 of of IN cord-319100-3gdawhfn 41 3 these these DT cord-319100-3gdawhfn 41 4 samples sample NNS cord-319100-3gdawhfn 41 5 were be VBD cord-319100-3gdawhfn 41 6 tested test VBN cord-319100-3gdawhfn 41 7 in in IN cord-319100-3gdawhfn 41 8 parallel parallel NN cord-319100-3gdawhfn 41 9 ( ( -LRB- cord-319100-3gdawhfn 41 10 see see VB cord-319100-3gdawhfn 41 11 below below RB cord-319100-3gdawhfn 41 12 ) ) -RRB- cord-319100-3gdawhfn 41 13 . . . cord-319100-3gdawhfn 42 1 To to TO cord-319100-3gdawhfn 42 2 extend extend VB cord-319100-3gdawhfn 42 3 the the DT cord-319100-3gdawhfn 42 4 study study NN cord-319100-3gdawhfn 42 5 beyond beyond IN cord-319100-3gdawhfn 42 6 SARS SARS NNP cord-319100-3gdawhfn 42 7 - - HYPH cord-319100-3gdawhfn 42 8 COV-2 COV-2 NNP cord-319100-3gdawhfn 42 9 virus virus NN cord-319100-3gdawhfn 42 10 , , , cord-319100-3gdawhfn 42 11 a a DT cord-319100-3gdawhfn 42 12 Type type NN cord-319100-3gdawhfn 42 13 A a DT cord-319100-3gdawhfn 42 14 influenza influenza NN cord-319100-3gdawhfn 42 15 virus virus NN cord-319100-3gdawhfn 42 16 , , , cord-319100-3gdawhfn 42 17 ( ( -LRB- cord-319100-3gdawhfn 42 18 L998 l998 UH cord-319100-3gdawhfn 42 19 , , , cord-319100-3gdawhfn 42 20 an an DT cord-319100-3gdawhfn 42 21 isolate isolate NN cord-319100-3gdawhfn 42 22 of of IN cord-319100-3gdawhfn 42 23 H10N7 h10n7 RB cord-319100-3gdawhfn 42 24 influenza influenza NN cord-319100-3gdawhfn 42 25 ( ( -LRB- cord-319100-3gdawhfn 42 26 12 12 CD cord-319100-3gdawhfn 42 27 ) ) -RRB- cord-319100-3gdawhfn 42 28 ) ) -RRB- cord-319100-3gdawhfn 42 29 , , , cord-319100-3gdawhfn 42 30 was be VBD cord-319100-3gdawhfn 42 31 used use VBN cord-319100-3gdawhfn 42 32 . . . cord-319100-3gdawhfn 43 1 RNA RNA NNP cord-319100-3gdawhfn 43 2 was be VBD cord-319100-3gdawhfn 43 3 purified purify VBN cord-319100-3gdawhfn 43 4 from from IN cord-319100-3gdawhfn 43 5 L998 l998 XX cord-319100-3gdawhfn 43 6 using use VBG cord-319100-3gdawhfn 43 7 standard standard JJ cord-319100-3gdawhfn 43 8 methods method NNS cord-319100-3gdawhfn 43 9 ( ( -LRB- cord-319100-3gdawhfn 43 10 see see VB cord-319100-3gdawhfn 43 11 below below RB cord-319100-3gdawhfn 43 12 ) ) -RRB- cord-319100-3gdawhfn 43 13 and and CC cord-319100-3gdawhfn 43 14 was be VBD cord-319100-3gdawhfn 43 15 diluted dilute VBN cord-319100-3gdawhfn 43 16 1/1000 1/1000 CD cord-319100-3gdawhfn 43 17 in in IN cord-319100-3gdawhfn 43 18 the the DT cord-319100-3gdawhfn 43 19 extraction extraction NN cord-319100-3gdawhfn 43 20 elution elution NN cord-319100-3gdawhfn 43 21 buffer buffer NN cord-319100-3gdawhfn 43 22 to to TO cord-319100-3gdawhfn 43 23 prepare prepare VB cord-319100-3gdawhfn 43 24 a a DT cord-319100-3gdawhfn 43 25 working work VBG cord-319100-3gdawhfn 43 26 stock stock NN cord-319100-3gdawhfn 43 27 . . . cord-319100-3gdawhfn 44 1 Dilutions dilution NNS cord-319100-3gdawhfn 44 2 of of IN cord-319100-3gdawhfn 44 3 this this DT cord-319100-3gdawhfn 44 4 purified purify VBN cord-319100-3gdawhfn 44 5 nucleic nucleic JJ cord-319100-3gdawhfn 44 6 acid acid NN cord-319100-3gdawhfn 44 7 preparation preparation NN cord-319100-3gdawhfn 44 8 were be VBD cord-319100-3gdawhfn 44 9 also also RB cord-319100-3gdawhfn 44 10 tested test VBN cord-319100-3gdawhfn 44 11 in in IN cord-319100-3gdawhfn 44 12 parallel parallel NN cord-319100-3gdawhfn 44 13 with with IN cord-319100-3gdawhfn 44 14 the the DT cord-319100-3gdawhfn 44 15 infectious infectious JJ cord-319100-3gdawhfn 44 16 reference reference NN cord-319100-3gdawhfn 44 17 virus virus NN cord-319100-3gdawhfn 44 18 L998 L998 '' cord-319100-3gdawhfn 44 19 as as IN cord-319100-3gdawhfn 44 20 described describe VBN cord-319100-3gdawhfn 44 21 for for IN cord-319100-3gdawhfn 44 22 the the DT cord-319100-3gdawhfn 44 23 SARS SARS NNP cord-319100-3gdawhfn 44 24 - - HYPH cord-319100-3gdawhfn 44 25 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 44 26 virus virus NN cord-319100-3gdawhfn 44 27 . . . cord-319100-3gdawhfn 45 1 Finally finally RB cord-319100-3gdawhfn 45 2 , , , cord-319100-3gdawhfn 45 3 a a DT cord-319100-3gdawhfn 45 4 preparation preparation NN cord-319100-3gdawhfn 45 5 of of IN cord-319100-3gdawhfn 45 6 bovine bovine JJ cord-319100-3gdawhfn 45 7 herpesvirus herpesvirus NN cord-319100-3gdawhfn 45 8 1 1 CD cord-319100-3gdawhfn 45 9 ( ( -LRB- cord-319100-3gdawhfn 45 10 BHV-1 BHV-1 NNPS cord-319100-3gdawhfn 45 11 , , , cord-319100-3gdawhfn 45 12 M631 m631 ADD cord-319100-3gdawhfn 45 13 ) ) -RRB- cord-319100-3gdawhfn 45 14 was be VBD cord-319100-3gdawhfn 45 15 similarly similarly RB cord-319100-3gdawhfn 45 16 prepared prepare VBN cord-319100-3gdawhfn 45 17 as as IN cord-319100-3gdawhfn 45 18 both both DT cord-319100-3gdawhfn 45 19 purified purify VBN cord-319100-3gdawhfn 45 20 DNA dna NN cord-319100-3gdawhfn 45 21 and and CC cord-319100-3gdawhfn 45 22 infectious infectious JJ cord-319100-3gdawhfn 45 23 virus virus NN cord-319100-3gdawhfn 45 24 to to TO cord-319100-3gdawhfn 45 25 determine determine VB cord-319100-3gdawhfn 45 26 the the DT cord-319100-3gdawhfn 45 27 influence influence NN cord-319100-3gdawhfn 45 28 of of IN cord-319100-3gdawhfn 45 29 transport transport NN cord-319100-3gdawhfn 45 30 media medium NNS cord-319100-3gdawhfn 45 31 on on IN cord-319100-3gdawhfn 45 32 a a DT cord-319100-3gdawhfn 45 33 DNA dna NN cord-319100-3gdawhfn 45 34 virus virus NN cord-319100-3gdawhfn 45 35 . . . cord-319100-3gdawhfn 46 1 The the DT cord-319100-3gdawhfn 46 2 study study NN cord-319100-3gdawhfn 46 3 included include VBD cord-319100-3gdawhfn 46 4 4 4 CD cord-319100-3gdawhfn 46 5 different different JJ cord-319100-3gdawhfn 46 6 VTMs vtm NNS cord-319100-3gdawhfn 46 7 . . . cord-319100-3gdawhfn 47 1 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 47 2 was be VBD cord-319100-3gdawhfn 47 3 an an DT cord-319100-3gdawhfn 47 4 " " `` cord-319100-3gdawhfn 47 5 in in IN cord-319100-3gdawhfn 47 6 - - HYPH cord-319100-3gdawhfn 47 7 house house NN cord-319100-3gdawhfn 47 8 " " '' cord-319100-3gdawhfn 47 9 product product NN cord-319100-3gdawhfn 47 10 based base VBN cord-319100-3gdawhfn 47 11 on on IN cord-319100-3gdawhfn 47 12 phosphate phosphate NN cord-319100-3gdawhfn 47 13 buffered buffer VBN cord-319100-3gdawhfn 47 14 saline saline NN cord-319100-3gdawhfn 47 15 ( ( -LRB- cord-319100-3gdawhfn 47 16 PBS PBS NNP cord-319100-3gdawhfn 47 17 pH pH NNP cord-319100-3gdawhfn 47 18 7.2 7.2 CD cord-319100-3gdawhfn 47 19 ) ) -RRB- cord-319100-3gdawhfn 47 20 supplemented supplement VBN cord-319100-3gdawhfn 47 21 with with IN cord-319100-3gdawhfn 47 22 0.5 0.5 CD cord-319100-3gdawhfn 47 23 % % NN cord-319100-3gdawhfn 47 24 gelatin gelatin NN cord-319100-3gdawhfn 47 25 ( ( -LRB- cord-319100-3gdawhfn 47 26 PBGS PBGS NNP cord-319100-3gdawhfn 47 27 ) ) -RRB- cord-319100-3gdawhfn 47 28 , , , cord-319100-3gdawhfn 47 29 antibiotics antibiotic NNS cord-319100-3gdawhfn 47 30 and and CC cord-319100-3gdawhfn 47 31 0.004 0.004 CD cord-319100-3gdawhfn 47 32 % % NN cord-319100-3gdawhfn 47 33 phenol phenol NN cord-319100-3gdawhfn 47 34 red red JJ cord-319100-3gdawhfn 47 35 as as IN cord-319100-3gdawhfn 47 36 a a DT cord-319100-3gdawhfn 47 37 pH ph NN cord-319100-3gdawhfn 47 38 indicator indicator NN cord-319100-3gdawhfn 47 39 . . . cord-319100-3gdawhfn 48 1 The the DT cord-319100-3gdawhfn 48 2 other other JJ cord-319100-3gdawhfn 48 3 three three CD cord-319100-3gdawhfn 48 4 were be VBD cord-319100-3gdawhfn 48 5 commercially commercially RB cord-319100-3gdawhfn 48 6 manufactured manufacture VBN cord-319100-3gdawhfn 49 1 VTMs VTMs NNP cord-319100-3gdawhfn 49 2 -VTM-2 -VTM-2 , cord-319100-3gdawhfn 49 3 and and CC cord-319100-3gdawhfn 49 4 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 49 5 are be VBP cord-319100-3gdawhfn 49 6 widely widely RB cord-319100-3gdawhfn 49 7 used use VBN cord-319100-3gdawhfn 49 8 , , , cord-319100-3gdawhfn 49 9 both both DT cord-319100-3gdawhfn 49 10 in in IN cord-319100-3gdawhfn 49 11 Australia Australia NNP cord-319100-3gdawhfn 49 12 as as RB cord-319100-3gdawhfn 49 13 well well RB cord-319100-3gdawhfn 49 14 as as IN cord-319100-3gdawhfn 49 15 many many JJ cord-319100-3gdawhfn 49 16 countries country NNS cord-319100-3gdawhfn 49 17 in in IN cord-319100-3gdawhfn 49 18 the the DT cord-319100-3gdawhfn 49 19 northern northern JJ cord-319100-3gdawhfn 49 20 hemisphere hemisphere NN cord-319100-3gdawhfn 49 21 ; ; : cord-319100-3gdawhfn 49 22 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 49 23 is be VBZ cord-319100-3gdawhfn 49 24 a a DT cord-319100-3gdawhfn 49 25 new new JJ cord-319100-3gdawhfn 49 26 Australian australian JJ cord-319100-3gdawhfn 49 27 manufactured manufacture VBN cord-319100-3gdawhfn 49 28 product product NN cord-319100-3gdawhfn 49 29 that that WDT cord-319100-3gdawhfn 49 30 is be VBZ cord-319100-3gdawhfn 49 31 undergoing undergo VBG cord-319100-3gdawhfn 49 32 evaluation evaluation NN cord-319100-3gdawhfn 49 33 . . . cord-319100-3gdawhfn 50 1 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 50 2 and and CC cord-319100-3gdawhfn 50 3 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 50 4 are be VBP cord-319100-3gdawhfn 50 5 believed believe VBN cord-319100-3gdawhfn 50 6 to to TO cord-319100-3gdawhfn 50 7 be be VB cord-319100-3gdawhfn 50 8 supplemented supplement VBN cord-319100-3gdawhfn 50 9 with with IN cord-319100-3gdawhfn 50 10 bovine bovine JJ cord-319100-3gdawhfn 50 11 serum serum NN cord-319100-3gdawhfn 50 12 albumen albuman NNS cord-319100-3gdawhfn 50 13 and and CC cord-319100-3gdawhfn 50 14 gelatin gelatin NN cord-319100-3gdawhfn 50 15 and and CC cord-319100-3gdawhfn 50 16 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 50 17 with with IN cord-319100-3gdawhfn 50 18 foetal foetal JJ cord-319100-3gdawhfn 50 19 bovine bovine JJ cord-319100-3gdawhfn 50 20 serum serum NN cord-319100-3gdawhfn 50 21 . . . cord-319100-3gdawhfn 51 1 Sterile sterile JJ cord-319100-3gdawhfn 51 2 phosphate phosphate NN cord-319100-3gdawhfn 51 3 buffered buffer VBN cord-319100-3gdawhfn 51 4 saline saline NN cord-319100-3gdawhfn 51 5 ( ( -LRB- cord-319100-3gdawhfn 51 6 PBS PBS NNP cord-319100-3gdawhfn 51 7 ) ) -RRB- cord-319100-3gdawhfn 51 8 , , . cord-319100-3gdawhfn 52 1 pH pH NNP cord-319100-3gdawhfn 52 2 7.2 7.2 CD cord-319100-3gdawhfn 52 3 was be VBD cord-319100-3gdawhfn 52 4 used use VBN cord-319100-3gdawhfn 52 5 as as IN cord-319100-3gdawhfn 52 6 a a DT cord-319100-3gdawhfn 52 7 control control NN cord-319100-3gdawhfn 52 8 solution solution NN cord-319100-3gdawhfn 52 9 . . . cord-319100-3gdawhfn 53 1 For for IN cord-319100-3gdawhfn 53 2 each each DT cord-319100-3gdawhfn 53 3 virus virus NN cord-319100-3gdawhfn 53 4 under under IN cord-319100-3gdawhfn 53 5 study study NN cord-319100-3gdawhfn 53 6 , , , cord-319100-3gdawhfn 53 7 the the DT cord-319100-3gdawhfn 53 8 concentrated concentrated JJ cord-319100-3gdawhfn 53 9 reference reference NN cord-319100-3gdawhfn 53 10 viruses virus NNS cord-319100-3gdawhfn 53 11 and and CC cord-319100-3gdawhfn 53 12 the the DT cord-319100-3gdawhfn 53 13 respective respective JJ cord-319100-3gdawhfn 53 14 purified purify VBN cord-319100-3gdawhfn 53 15 nucleic nucleic JJ cord-319100-3gdawhfn 53 16 acids acid NNS cord-319100-3gdawhfn 53 17 were be VBD cord-319100-3gdawhfn 53 18 diluted dilute VBN cord-319100-3gdawhfn 53 19 in in IN cord-319100-3gdawhfn 53 20 either either DT cord-319100-3gdawhfn 53 21 phosphate phosphate NN cord-319100-3gdawhfn 53 22 buffered buffer VBN cord-319100-3gdawhfn 53 23 saline saline NN cord-319100-3gdawhfn 53 24 ( ( -LRB- cord-319100-3gdawhfn 53 25 PBS PBS NNP cord-319100-3gdawhfn 53 26 ) ) -RRB- cord-319100-3gdawhfn 53 27 or or CC cord-319100-3gdawhfn 53 28 one one CD cord-319100-3gdawhfn 53 29 of of IN cord-319100-3gdawhfn 53 30 the the DT cord-319100-3gdawhfn 53 31 VTM VTM NNP cord-319100-3gdawhfn 53 32 solutions solution NNS cord-319100-3gdawhfn 53 33 and and CC cord-319100-3gdawhfn 53 34 were be VBD cord-319100-3gdawhfn 53 35 then then RB cord-319100-3gdawhfn 53 36 subjected subject VBN cord-319100-3gdawhfn 53 37 to to IN cord-319100-3gdawhfn 53 38 nucleic nucleic JJ cord-319100-3gdawhfn 53 39 acid acid NN cord-319100-3gdawhfn 53 40 extraction extraction NN cord-319100-3gdawhfn 53 41 and and CC cord-319100-3gdawhfn 53 42 testing testing NN cord-319100-3gdawhfn 53 43 by by IN cord-319100-3gdawhfn 53 44 qPCR qPCR NNP cord-319100-3gdawhfn 53 45 . . . cord-319100-3gdawhfn 54 1 Specifically specifically RB cord-319100-3gdawhfn 54 2 , , , cord-319100-3gdawhfn 54 3 dilutions dilution NNS cord-319100-3gdawhfn 54 4 of of IN cord-319100-3gdawhfn 54 5 the the DT cord-319100-3gdawhfn 54 6 purified purify VBN cord-319100-3gdawhfn 54 7 SARS SARS NNP cord-319100-3gdawhfn 54 8 - - HYPH cord-319100-3gdawhfn 54 9 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 54 10 RNA RNA NNP cord-319100-3gdawhfn 54 11 ( ( -LRB- cord-319100-3gdawhfn 54 12 R7215 R7215 NNP cord-319100-3gdawhfn 54 13 ) ) -RRB- cord-319100-3gdawhfn 54 14 were be VBD cord-319100-3gdawhfn 54 15 prepared prepare VBN cord-319100-3gdawhfn 54 16 by by IN cord-319100-3gdawhfn 54 17 adding add VBG cord-319100-3gdawhfn 54 18 either either CC cord-319100-3gdawhfn 54 19 50uL 50ul CD cord-319100-3gdawhfn 54 20 of of IN cord-319100-3gdawhfn 54 21 R7215 R7215 NNP cord-319100-3gdawhfn 54 22 to to IN cord-319100-3gdawhfn 54 23 450uL 450ul CD cord-319100-3gdawhfn 54 24 for for IN cord-319100-3gdawhfn 54 25 a a DT cord-319100-3gdawhfn 54 26 1/10 1/10 CD cord-319100-3gdawhfn 54 27 dilution dilution NN cord-319100-3gdawhfn 54 28 of of IN cord-319100-3gdawhfn 54 29 the the DT cord-319100-3gdawhfn 54 30 VTM VTM NNP cord-319100-3gdawhfn 54 31 under under IN cord-319100-3gdawhfn 54 32 study study NN cord-319100-3gdawhfn 54 33 or or CC cord-319100-3gdawhfn 54 34 , , , cord-319100-3gdawhfn 54 35 for for IN cord-319100-3gdawhfn 54 36 1/2 1/2 CD cord-319100-3gdawhfn 54 37 dilutions dilution NNS cord-319100-3gdawhfn 54 38 , , , cord-319100-3gdawhfn 54 39 100uL 100ul CD cord-319100-3gdawhfn 54 40 of of IN cord-319100-3gdawhfn 54 41 diluted dilute VBN cord-319100-3gdawhfn 54 42 R7215 R7215 NNP cord-319100-3gdawhfn 54 43 to to IN cord-319100-3gdawhfn 54 44 an an DT cord-319100-3gdawhfn 54 45 equal equal JJ cord-319100-3gdawhfn 54 46 volume volume NN cord-319100-3gdawhfn 54 47 of of IN cord-319100-3gdawhfn 54 48 the the DT cord-319100-3gdawhfn 54 49 respective respective JJ cord-319100-3gdawhfn 54 50 VTM VTM NNP cord-319100-3gdawhfn 54 51 . . . cord-319100-3gdawhfn 55 1 In in IN cord-319100-3gdawhfn 55 2 the the DT cord-319100-3gdawhfn 55 3 pilot pilot NN cord-319100-3gdawhfn 55 4 experiment experiment NN cord-319100-3gdawhfn 55 5 , , , cord-319100-3gdawhfn 55 6 only only RB cord-319100-3gdawhfn 55 7 VTMs vtms NN cord-319100-3gdawhfn 55 8 1 1 CD cord-319100-3gdawhfn 55 9 - - SYM cord-319100-3gdawhfn 55 10 3 3 CD cord-319100-3gdawhfn 55 11 were be VBD cord-319100-3gdawhfn 55 12 included include VBN cord-319100-3gdawhfn 55 13 . . . cord-319100-3gdawhfn 56 1 Within within IN cord-319100-3gdawhfn 56 2 15 15 CD cord-319100-3gdawhfn 56 3 minutes minute NNS cord-319100-3gdawhfn 56 4 of of IN cord-319100-3gdawhfn 56 5 preparation preparation NN cord-319100-3gdawhfn 56 6 of of IN cord-319100-3gdawhfn 56 7 the the DT cord-319100-3gdawhfn 56 8 dilutions dilution NNS cord-319100-3gdawhfn 56 9 of of IN cord-319100-3gdawhfn 56 10 RNA RNA NNP cord-319100-3gdawhfn 56 11 in in IN cord-319100-3gdawhfn 56 12 each each DT cord-319100-3gdawhfn 56 13 VTM VTM NNP cord-319100-3gdawhfn 56 14 , , , cord-319100-3gdawhfn 56 15 the the DT cord-319100-3gdawhfn 56 16 diluted dilute VBN cord-319100-3gdawhfn 56 17 samples sample NNS cord-319100-3gdawhfn 56 18 were be VBD cord-319100-3gdawhfn 56 19 extracted extract VBN cord-319100-3gdawhfn 56 20 and and CC cord-319100-3gdawhfn 56 21 tested test VBN cord-319100-3gdawhfn 56 22 by by IN cord-319100-3gdawhfn 56 23 qRT qRT NNP cord-319100-3gdawhfn 56 24 - - HYPH cord-319100-3gdawhfn 56 25 PCR PCR NNP cord-319100-3gdawhfn 56 26 as as IN cord-319100-3gdawhfn 56 27 described describe VBN cord-319100-3gdawhfn 56 28 below below RB cord-319100-3gdawhfn 56 29 . . . cord-319100-3gdawhfn 57 1 Subsequently subsequently RB cord-319100-3gdawhfn 57 2 , , , cord-319100-3gdawhfn 57 3 this this DT cord-319100-3gdawhfn 57 4 pilot pilot NN cord-319100-3gdawhfn 57 5 experiment experiment NN cord-319100-3gdawhfn 57 6 was be VBD cord-319100-3gdawhfn 57 7 repeated repeat VBN cord-319100-3gdawhfn 57 8 with with IN cord-319100-3gdawhfn 57 9 the the DT cord-319100-3gdawhfn 57 10 inclusion inclusion NN cord-319100-3gdawhfn 57 11 of of IN cord-319100-3gdawhfn 57 12 PBS PBS NNP cord-319100-3gdawhfn 57 13 and and CC cord-319100-3gdawhfn 57 14 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 57 15 , , , cord-319100-3gdawhfn 57 16 as as RB cord-319100-3gdawhfn 57 17 well well RB cord-319100-3gdawhfn 57 18 as as IN cord-319100-3gdawhfn 57 19 a a DT cord-319100-3gdawhfn 57 20 series series NN cord-319100-3gdawhfn 57 21 of of IN cord-319100-3gdawhfn 57 22 dilutions dilution NNS cord-319100-3gdawhfn 57 23 of of IN cord-319100-3gdawhfn 57 24 patient patient JJ cord-319100-3gdawhfn 57 25 sample sample NN cord-319100-3gdawhfn 57 26 P66 P66 NNP cord-319100-3gdawhfn 57 27 . . . cord-319100-3gdawhfn 58 1 These these DT cord-319100-3gdawhfn 58 2 dilutions dilution NNS cord-319100-3gdawhfn 58 3 of of IN cord-319100-3gdawhfn 58 4 RNA RNA NNP cord-319100-3gdawhfn 58 5 and and CC cord-319100-3gdawhfn 58 6 virus virus NN cord-319100-3gdawhfn 58 7 were be VBD cord-319100-3gdawhfn 58 8 then then RB cord-319100-3gdawhfn 58 9 extracted extract VBN cord-319100-3gdawhfn 58 10 within within IN cord-319100-3gdawhfn 58 11 45 45 CD cord-319100-3gdawhfn 58 12 minutes minute NNS cord-319100-3gdawhfn 58 13 of of IN cord-319100-3gdawhfn 58 14 preparation preparation NN cord-319100-3gdawhfn 58 15 and and CC cord-319100-3gdawhfn 58 16 tested test VBN cord-319100-3gdawhfn 58 17 in in IN cord-319100-3gdawhfn 58 18 several several JJ cord-319100-3gdawhfn 58 19 SARS SARS NNP cord-319100-3gdawhfn 58 20 - - HYPH cord-319100-3gdawhfn 58 21 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 58 22 qRT qRT NNP cord-319100-3gdawhfn 58 23 - - HYPH cord-319100-3gdawhfn 58 24 PCR PCR NNP cord-319100-3gdawhfn 58 25 assays assay NNS cord-319100-3gdawhfn 58 26 . . . cord-319100-3gdawhfn 59 1 Later later RB cord-319100-3gdawhfn 59 2 , , , cord-319100-3gdawhfn 59 3 less less JJR cord-319100-3gdawhfn 59 4 dilute dilute VB cord-319100-3gdawhfn 59 5 preparations preparation NNS cord-319100-3gdawhfn 59 6 of of IN cord-319100-3gdawhfn 59 7 R7215 R7215 NNP cord-319100-3gdawhfn 59 8 were be VBD cord-319100-3gdawhfn 59 9 made make VBN cord-319100-3gdawhfn 59 10 so so IN cord-319100-3gdawhfn 59 11 that that IN cord-319100-3gdawhfn 59 12 the the DT cord-319100-3gdawhfn 59 13 impact impact NN cord-319100-3gdawhfn 59 14 of of IN cord-319100-3gdawhfn 59 15 the the DT cord-319100-3gdawhfn 59 16 VTMs vtm NNS cord-319100-3gdawhfn 59 17 on on IN cord-319100-3gdawhfn 59 18 higher high JJR cord-319100-3gdawhfn 59 19 concentrations concentration NNS cord-319100-3gdawhfn 59 20 of of IN cord-319100-3gdawhfn 59 21 SARS SARS NNP cord-319100-3gdawhfn 59 22 - - HYPH cord-319100-3gdawhfn 59 23 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 59 24 RNA RNA NNP cord-319100-3gdawhfn 59 25 could could MD cord-319100-3gdawhfn 59 26 also also RB cord-319100-3gdawhfn 59 27 be be VB cord-319100-3gdawhfn 59 28 assessed assess VBN cord-319100-3gdawhfn 59 29 . . . cord-319100-3gdawhfn 60 1 To to TO cord-319100-3gdawhfn 60 2 obtain obtain VB cord-319100-3gdawhfn 60 3 sufficient sufficient JJ cord-319100-3gdawhfn 60 4 VTM VTM NNP cord-319100-3gdawhfn 60 5 as as IN cord-319100-3gdawhfn 60 6 a a DT cord-319100-3gdawhfn 60 7 diluent diluent NN cord-319100-3gdawhfn 60 8 for for IN cord-319100-3gdawhfn 60 9 this this DT cord-319100-3gdawhfn 60 10 study study NN cord-319100-3gdawhfn 60 11 , , , cord-319100-3gdawhfn 60 12 the the DT cord-319100-3gdawhfn 60 13 contents content NNS cord-319100-3gdawhfn 60 14 ( ( -LRB- cord-319100-3gdawhfn 60 15 2.5 2.5 CD cord-319100-3gdawhfn 60 16 - - SYM cord-319100-3gdawhfn 60 17 3mL 3ml CD cord-319100-3gdawhfn 60 18 ) ) -RRB- cord-319100-3gdawhfn 60 19 of of IN cord-319100-3gdawhfn 60 20 2 2 CD cord-319100-3gdawhfn 60 21 vials vial NNS cord-319100-3gdawhfn 60 22 of of IN cord-319100-3gdawhfn 60 23 VTM VTM NNP cord-319100-3gdawhfn 60 24 were be VBD cord-319100-3gdawhfn 60 25 pooled pool VBN cord-319100-3gdawhfn 60 26 and and CC cord-319100-3gdawhfn 60 27 mixed mix VBN cord-319100-3gdawhfn 60 28 . . . cord-319100-3gdawhfn 61 1 To to TO cord-319100-3gdawhfn 61 2 investigate investigate VB cord-319100-3gdawhfn 61 3 whether whether IN cord-319100-3gdawhfn 61 4 the the DT cord-319100-3gdawhfn 61 5 results result NNS cord-319100-3gdawhfn 61 6 obtained obtain VBN cord-319100-3gdawhfn 61 7 from from IN cord-319100-3gdawhfn 61 8 the the DT cord-319100-3gdawhfn 61 9 experiments experiment NNS cord-319100-3gdawhfn 61 10 involving involve VBG cord-319100-3gdawhfn 61 11 SARS SARS NNP cord-319100-3gdawhfn 61 12 - - HYPH cord-319100-3gdawhfn 61 13 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 61 14 were be VBD cord-319100-3gdawhfn 61 15 applicable applicable JJ cord-319100-3gdawhfn 61 16 to to IN cord-319100-3gdawhfn 61 17 other other JJ cord-319100-3gdawhfn 61 18 viruses virus NNS cord-319100-3gdawhfn 61 19 , , , cord-319100-3gdawhfn 61 20 the the DT cord-319100-3gdawhfn 61 21 same same JJ cord-319100-3gdawhfn 61 22 experimental experimental JJ cord-319100-3gdawhfn 61 23 design design NN cord-319100-3gdawhfn 61 24 was be VBD cord-319100-3gdawhfn 61 25 applied apply VBN cord-319100-3gdawhfn 61 26 to to IN cord-319100-3gdawhfn 61 27 a a DT cord-319100-3gdawhfn 61 28 Type type NN cord-319100-3gdawhfn 61 29 A a DT cord-319100-3gdawhfn 61 30 influenza influenza NN cord-319100-3gdawhfn 61 31 virus virus NN cord-319100-3gdawhfn 61 32 and and CC cord-319100-3gdawhfn 61 33 a a DT cord-319100-3gdawhfn 61 34 herpesvirus herpesvirus NN cord-319100-3gdawhfn 61 35 and and CC cord-319100-3gdawhfn 61 36 the the DT cord-319100-3gdawhfn 61 37 respective respective JJ cord-319100-3gdawhfn 61 38 purified purify VBN cord-319100-3gdawhfn 61 39 viral viral JJ cord-319100-3gdawhfn 61 40 nucleic nucleic JJ cord-319100-3gdawhfn 61 41 acid acid NN cord-319100-3gdawhfn 61 42 extracts extract NNS cord-319100-3gdawhfn 61 43 . . . cord-319100-3gdawhfn 62 1 The the DT cord-319100-3gdawhfn 62 2 starting start VBG cord-319100-3gdawhfn 62 3 concentrations concentration NNS cord-319100-3gdawhfn 62 4 of of IN cord-319100-3gdawhfn 62 5 nucleic nucleic JJ cord-319100-3gdawhfn 62 6 acid acid NN cord-319100-3gdawhfn 62 7 and and CC cord-319100-3gdawhfn 62 8 whole whole JJ cord-319100-3gdawhfn 62 9 virus virus NN cord-319100-3gdawhfn 62 10 were be VBD cord-319100-3gdawhfn 62 11 adjusted adjust VBN cord-319100-3gdawhfn 62 12 so so IN cord-319100-3gdawhfn 62 13 that that IN cord-319100-3gdawhfn 62 14 the the DT cord-319100-3gdawhfn 62 15 levels level NNS cord-319100-3gdawhfn 62 16 of of IN cord-319100-3gdawhfn 62 17 nucleic nucleic JJ cord-319100-3gdawhfn 62 18 acid acid NN cord-319100-3gdawhfn 62 19 ( ( -LRB- cord-319100-3gdawhfn 62 20 based base VBN cord-319100-3gdawhfn 62 21 on on IN cord-319100-3gdawhfn 62 22 cycle cycle NN cord-319100-3gdawhfn 62 23 - - HYPH cord-319100-3gdawhfn 62 24 threshold threshold NN cord-319100-3gdawhfn 62 25 ( ( -LRB- cord-319100-3gdawhfn 62 26 Ct Ct NNP cord-319100-3gdawhfn 62 27 ) ) -RRB- cord-319100-3gdawhfn 62 28 values value NNS cord-319100-3gdawhfn 62 29 ) ) -RRB- cord-319100-3gdawhfn 62 30 were be VBD cord-319100-3gdawhfn 62 31 very very RB cord-319100-3gdawhfn 62 32 close close JJ cord-319100-3gdawhfn 62 33 to to IN cord-319100-3gdawhfn 62 34 those those DT cord-319100-3gdawhfn 62 35 used use VBN cord-319100-3gdawhfn 62 36 in in IN cord-319100-3gdawhfn 62 37 the the DT cord-319100-3gdawhfn 62 38 SARS SARS NNP cord-319100-3gdawhfn 62 39 - - HYPH cord-319100-3gdawhfn 62 40 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 62 41 experiments experiment NNS cord-319100-3gdawhfn 62 42 . . . cord-319100-3gdawhfn 63 1 In in IN cord-319100-3gdawhfn 63 2 each each DT cord-319100-3gdawhfn 63 3 instance instance NN cord-319100-3gdawhfn 63 4 each each DT cord-319100-3gdawhfn 63 5 diluted dilute VBN cord-319100-3gdawhfn 63 6 sample sample NN cord-319100-3gdawhfn 63 7 ( ( -LRB- cord-319100-3gdawhfn 63 8 nucleic nucleic JJ cord-319100-3gdawhfn 63 9 acid acid NN cord-319100-3gdawhfn 63 10 or or CC cord-319100-3gdawhfn 63 11 whole whole JJ cord-319100-3gdawhfn 63 12 virus virus NN cord-319100-3gdawhfn 63 13 ) ) -RRB- cord-319100-3gdawhfn 63 14 was be VBD cord-319100-3gdawhfn 63 15 extracted extract VBN cord-319100-3gdawhfn 63 16 and and CC cord-319100-3gdawhfn 63 17 tested test VBN cord-319100-3gdawhfn 63 18 within within IN cord-319100-3gdawhfn 63 19 45 45 CD cord-319100-3gdawhfn 63 20 minutes minute NNS cord-319100-3gdawhfn 63 21 of of IN cord-319100-3gdawhfn 63 22 preparation preparation NN cord-319100-3gdawhfn 63 23 of of IN cord-319100-3gdawhfn 63 24 the the DT cord-319100-3gdawhfn 63 25 dilutions dilution NNS cord-319100-3gdawhfn 63 26 . . . cord-319100-3gdawhfn 64 1 As as RB cord-319100-3gdawhfn 64 2 well well RB cord-319100-3gdawhfn 64 3 as as IN cord-319100-3gdawhfn 64 4 testing test VBG cord-319100-3gdawhfn 64 5 the the DT cord-319100-3gdawhfn 64 6 whole whole JJ cord-319100-3gdawhfn 64 7 virus virus NN cord-319100-3gdawhfn 64 8 & & CC cord-319100-3gdawhfn 64 9 purified purify VBN cord-319100-3gdawhfn 64 10 nucleic nucleic JJ cord-319100-3gdawhfn 64 11 acid acid NN cord-319100-3gdawhfn 64 12 combinations combination NNS cord-319100-3gdawhfn 64 13 , , , cord-319100-3gdawhfn 64 14 the the DT cord-319100-3gdawhfn 64 15 impact impact NN cord-319100-3gdawhfn 64 16 of of IN cord-319100-3gdawhfn 64 17 the the DT cord-319100-3gdawhfn 64 18 different different JJ cord-319100-3gdawhfn 64 19 VTMs vtm NNS cord-319100-3gdawhfn 64 20 on on IN cord-319100-3gdawhfn 64 21 the the DT cord-319100-3gdawhfn 64 22 exogenous exogenous JJ cord-319100-3gdawhfn 64 23 RNA RNA NNP cord-319100-3gdawhfn 64 24 internal internal JJ cord-319100-3gdawhfn 64 25 control control NN cord-319100-3gdawhfn 64 26 ( ( -LRB- cord-319100-3gdawhfn 64 27 XIPC XIPC NNP cord-319100-3gdawhfn 64 28 ) ) -RRB- cord-319100-3gdawhfn 64 29 were be VBD cord-319100-3gdawhfn 64 30 also also RB cord-319100-3gdawhfn 64 31 assessed assess VBN cord-319100-3gdawhfn 64 32 by by IN cord-319100-3gdawhfn 64 33 adding add VBG cord-319100-3gdawhfn 64 34 1,000 1,000 CD cord-319100-3gdawhfn 64 35 or or CC cord-319100-3gdawhfn 64 36 10,000 10,000 CD cord-319100-3gdawhfn 64 37 copies copy NNS cord-319100-3gdawhfn 64 38 of of IN cord-319100-3gdawhfn 64 39 the the DT cord-319100-3gdawhfn 64 40 XIPC XIPC NNP cord-319100-3gdawhfn 64 41 to to IN cord-319100-3gdawhfn 64 42 100uL 100ul CD cord-319100-3gdawhfn 64 43 of of IN cord-319100-3gdawhfn 64 44 each each DT cord-319100-3gdawhfn 64 45 VTM VTM NNP cord-319100-3gdawhfn 64 46 and and CC cord-319100-3gdawhfn 64 47 testing testing NN cord-319100-3gdawhfn 64 48 immediately immediately RB cord-319100-3gdawhfn 64 49 or or CC cord-319100-3gdawhfn 64 50 after after IN cord-319100-3gdawhfn 64 51 the the DT cord-319100-3gdawhfn 64 52 dilutions dilution NNS cord-319100-3gdawhfn 64 53 had have VBD cord-319100-3gdawhfn 64 54 been be VBN cord-319100-3gdawhfn 64 55 held hold VBN cord-319100-3gdawhfn 64 56 at at IN cord-319100-3gdawhfn 64 57 25 25 CD cord-319100-3gdawhfn 64 58 o o NN cord-319100-3gdawhfn 64 59 C c NN cord-319100-3gdawhfn 64 60 for for IN cord-319100-3gdawhfn 64 61 48 48 CD cord-319100-3gdawhfn 64 62 hours hour NNS cord-319100-3gdawhfn 64 63 . . . cord-319100-3gdawhfn 65 1 Finally finally RB cord-319100-3gdawhfn 65 2 , , , cord-319100-3gdawhfn 65 3 to to TO cord-319100-3gdawhfn 65 4 confirm confirm VB cord-319100-3gdawhfn 65 5 that that IN cord-319100-3gdawhfn 65 6 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 65 7 can can MD cord-319100-3gdawhfn 65 8 provide provide VB cord-319100-3gdawhfn 65 9 a a DT cord-319100-3gdawhfn 65 10 high high JJ cord-319100-3gdawhfn 65 11 level level NN cord-319100-3gdawhfn 65 12 of of IN cord-319100-3gdawhfn 65 13 stability stability NN cord-319100-3gdawhfn 65 14 for for IN cord-319100-3gdawhfn 65 15 clinical clinical JJ cord-319100-3gdawhfn 65 16 samples sample NNS cord-319100-3gdawhfn 65 17 , , , cord-319100-3gdawhfn 65 18 15 15 CD cord-319100-3gdawhfn 65 19 swabs swab NNS cord-319100-3gdawhfn 65 20 from from IN cord-319100-3gdawhfn 65 21 SARS SARS NNP cord-319100-3gdawhfn 65 22 - - HYPH cord-319100-3gdawhfn 65 23 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 65 24 infected infected JJ cord-319100-3gdawhfn 65 25 patients patient NNS cord-319100-3gdawhfn 65 26 were be VBD cord-319100-3gdawhfn 65 27 removed remove VBN cord-319100-3gdawhfn 65 28 from from IN cord-319100-3gdawhfn 65 29 the the DT cord-319100-3gdawhfn 65 30 transport transport NN cord-319100-3gdawhfn 65 31 medium medium NN cord-319100-3gdawhfn 65 32 in in IN cord-319100-3gdawhfn 65 33 which which WDT cord-319100-3gdawhfn 65 34 they -PRON- PRP cord-319100-3gdawhfn 65 35 had have VBD cord-319100-3gdawhfn 65 36 been be VBN cord-319100-3gdawhfn 65 37 collected collect VBN cord-319100-3gdawhfn 65 38 and and CC cord-319100-3gdawhfn 65 39 were be VBD cord-319100-3gdawhfn 65 40 placed place VBN cord-319100-3gdawhfn 65 41 in in IN cord-319100-3gdawhfn 65 42 new new JJ cord-319100-3gdawhfn 65 43 vials vial NNS cord-319100-3gdawhfn 65 44 containing contain VBG cord-319100-3gdawhfn 65 45 1ml 1ml NN cord-319100-3gdawhfn 65 46 of of IN cord-319100-3gdawhfn 65 47 VTM-1 VTM-1 NNS cord-319100-3gdawhfn 65 48 and and CC cord-319100-3gdawhfn 65 49 were be VBD cord-319100-3gdawhfn 65 50 then then RB cord-319100-3gdawhfn 65 51 held hold VBN cord-319100-3gdawhfn 65 52 at at IN cord-319100-3gdawhfn 65 53 4 4 CD cord-319100-3gdawhfn 65 54 o o NN cord-319100-3gdawhfn 65 55 C c NN cord-319100-3gdawhfn 65 56 for for IN cord-319100-3gdawhfn 65 57 46 46 CD cord-319100-3gdawhfn 65 58 - - SYM cord-319100-3gdawhfn 65 59 49 49 CD cord-319100-3gdawhfn 65 60 days day NNS cord-319100-3gdawhfn 65 61 before before IN cord-319100-3gdawhfn 65 62 testing testing NN cord-319100-3gdawhfn 65 63 . . . cord-319100-3gdawhfn 66 1 Nucleic nucleic JJ cord-319100-3gdawhfn 66 2 acid acid NN cord-319100-3gdawhfn 66 3 extraction extraction NN cord-319100-3gdawhfn 66 4 and and CC cord-319100-3gdawhfn 66 5 PCR PCR NNP cord-319100-3gdawhfn 66 6 assays assay NNS cord-319100-3gdawhfn 66 7 : : : cord-319100-3gdawhfn 67 1 Total total JJ cord-319100-3gdawhfn 67 2 nucleic nucleic JJ cord-319100-3gdawhfn 67 3 acid acid NN cord-319100-3gdawhfn 67 4 was be VBD cord-319100-3gdawhfn 67 5 extracted extract VBN cord-319100-3gdawhfn 67 6 from from IN cord-319100-3gdawhfn 67 7 50uL 50ul CD cord-319100-3gdawhfn 67 8 of of IN cord-319100-3gdawhfn 67 9 each each DT cord-319100-3gdawhfn 67 10 sample sample NN cord-319100-3gdawhfn 67 11 ( ( -LRB- cord-319100-3gdawhfn 67 12 including include VBG cord-319100-3gdawhfn 67 13 dilutions dilution NNS cord-319100-3gdawhfn 67 14 of of IN cord-319100-3gdawhfn 67 15 the the DT cord-319100-3gdawhfn 67 16 already already RB cord-319100-3gdawhfn 67 17 purified purify VBN cord-319100-3gdawhfn 67 18 RNA RNA NNP cord-319100-3gdawhfn 67 19 or or CC cord-319100-3gdawhfn 67 20 DNA DNA NNP cord-319100-3gdawhfn 67 21 ) ) -RRB- cord-319100-3gdawhfn 67 22 with with IN cord-319100-3gdawhfn 67 23 a a DT cord-319100-3gdawhfn 67 24 magnetic magnetic JJ cord-319100-3gdawhfn 67 25 beadbased beadbase VBN cord-319100-3gdawhfn 67 26 viral viral JJ cord-319100-3gdawhfn 67 27 RNA RNA NNP cord-319100-3gdawhfn 67 28 extraction extraction NN cord-319100-3gdawhfn 67 29 kit kit NN cord-319100-3gdawhfn 67 30 ( ( -LRB- cord-319100-3gdawhfn 67 31 MagMax96 MagMax96 NNP cord-319100-3gdawhfn 68 1 Viral Viral NNP cord-319100-3gdawhfn 68 2 RNA RNA NNP cord-319100-3gdawhfn 69 1 -Ambion -ambion LS cord-319100-3gdawhfn 69 2 ) ) -RRB- cord-319100-3gdawhfn 69 3 that that WDT cord-319100-3gdawhfn 69 4 is be VBZ cord-319100-3gdawhfn 69 5 run run VBN cord-319100-3gdawhfn 69 6 on on IN cord-319100-3gdawhfn 69 7 a a DT cord-319100-3gdawhfn 69 8 Kingfisher-96 Kingfisher-96 NNP cord-319100-3gdawhfn 69 9 magnetic magnetic JJ cord-319100-3gdawhfn 69 10 particle particle NN cord-319100-3gdawhfn 69 11 handling handle VBG cord-319100-3gdawhfn 69 12 system system NN cord-319100-3gdawhfn 69 13 ( ( -LRB- cord-319100-3gdawhfn 69 14 ThermoFisher ThermoFisher NNP cord-319100-3gdawhfn 69 15 ) ) -RRB- cord-319100-3gdawhfn 69 16 . . . cord-319100-3gdawhfn 70 1 After after IN cord-319100-3gdawhfn 70 2 purification purification NN cord-319100-3gdawhfn 70 3 the the DT cord-319100-3gdawhfn 70 4 nucleic nucleic JJ cord-319100-3gdawhfn 70 5 acid acid NN cord-319100-3gdawhfn 70 6 was be VBD cord-319100-3gdawhfn 70 7 eluted elute VBN cord-319100-3gdawhfn 70 8 in in IN cord-319100-3gdawhfn 70 9 50uL 50ul CD cord-319100-3gdawhfn 70 10 of of IN cord-319100-3gdawhfn 70 11 kit kit NN cord-319100-3gdawhfn 70 12 elution elution NN cord-319100-3gdawhfn 70 13 buffer buffer NN cord-319100-3gdawhfn 70 14 and and CC cord-319100-3gdawhfn 70 15 5uL 5ul CD cord-319100-3gdawhfn 70 16 run run NN cord-319100-3gdawhfn 70 17 in in IN cord-319100-3gdawhfn 70 18 the the DT cord-319100-3gdawhfn 70 19 appropriate appropriate JJ cord-319100-3gdawhfn 70 20 qRT qRT NNP cord-319100-3gdawhfn 70 21 - - HYPH cord-319100-3gdawhfn 70 22 PCR PCR NNP cord-319100-3gdawhfn 70 23 . . . cord-319100-3gdawhfn 71 1 The the DT cord-319100-3gdawhfn 71 2 primers primer NNS cord-319100-3gdawhfn 71 3 and and CC cord-319100-3gdawhfn 71 4 probe probe NN cord-319100-3gdawhfn 71 5 were be VBD cord-319100-3gdawhfn 71 6 directed direct VBN cord-319100-3gdawhfn 71 7 at at IN cord-319100-3gdawhfn 71 8 the the DT cord-319100-3gdawhfn 71 9 SARS SARS NNP cord-319100-3gdawhfn 71 10 - - HYPH cord-319100-3gdawhfn 71 11 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 71 12 E e NN cord-319100-3gdawhfn 71 13 gene gene NN cord-319100-3gdawhfn 71 14 ( ( -LRB- cord-319100-3gdawhfn 71 15 13 13 CD cord-319100-3gdawhfn 71 16 ) ) -RRB- cord-319100-3gdawhfn 71 17 and and CC cord-319100-3gdawhfn 71 18 the the DT cord-319100-3gdawhfn 71 19 IP4 IP4 NNP cord-319100-3gdawhfn 71 20 assay assay NN cord-319100-3gdawhfn 71 21 targeting target VBG cord-319100-3gdawhfn 71 22 the the DT cord-319100-3gdawhfn 71 23 RdRp RdRp NNP cord-319100-3gdawhfn 71 24 gene gene NN cord-319100-3gdawhfn 71 25 ( ( -LRB- cord-319100-3gdawhfn 71 26 14 14 CD cord-319100-3gdawhfn 71 27 ) ) -RRB- cord-319100-3gdawhfn 71 28 as as IN cord-319100-3gdawhfn 71 29 developed develop VBN cord-319100-3gdawhfn 71 30 by by IN cord-319100-3gdawhfn 71 31 the the DT cord-319100-3gdawhfn 71 32 Institut Institut NNP cord-319100-3gdawhfn 71 33 Pasteur Pasteur NNP cord-319100-3gdawhfn 71 34 ( ( -LRB- cord-319100-3gdawhfn 71 35 IP IP NNP cord-319100-3gdawhfn 71 36 ) ) -RRB- cord-319100-3gdawhfn 71 37 , , , cord-319100-3gdawhfn 71 38 Paris Paris NNP cord-319100-3gdawhfn 71 39 , , , cord-319100-3gdawhfn 71 40 France France NNP cord-319100-3gdawhfn 71 41 and and CC cord-319100-3gdawhfn 71 42 recommended recommend VBN cord-319100-3gdawhfn 71 43 by by IN cord-319100-3gdawhfn 71 44 the the DT cord-319100-3gdawhfn 71 45 World World NNP cord-319100-3gdawhfn 71 46 Health Health NNP cord-319100-3gdawhfn 71 47 Organisation Organisation NNP cord-319100-3gdawhfn 71 48 . . . cord-319100-3gdawhfn 72 1 The the DT cord-319100-3gdawhfn 72 2 SARS SARS NNP cord-319100-3gdawhfn 72 3 - - HYPH cord-319100-3gdawhfn 72 4 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 72 5 primers primer NNS cord-319100-3gdawhfn 72 6 and and CC cord-319100-3gdawhfn 72 7 probe probe NN cord-319100-3gdawhfn 72 8 were be VBD cord-319100-3gdawhfn 72 9 used use VBN cord-319100-3gdawhfn 72 10 in in IN cord-319100-3gdawhfn 72 11 a a DT cord-319100-3gdawhfn 72 12 triplex triplex JJ cord-319100-3gdawhfn 72 13 assay assay NN cord-319100-3gdawhfn 72 14 format format NN cord-319100-3gdawhfn 72 15 with with IN cord-319100-3gdawhfn 72 16 the the DT cord-319100-3gdawhfn 72 17 inclusion inclusion NN cord-319100-3gdawhfn 72 18 of of IN cord-319100-3gdawhfn 72 19 the the DT cord-319100-3gdawhfn 72 20 exogenous exogenous JJ cord-319100-3gdawhfn 72 21 RNA RNA NNP cord-319100-3gdawhfn 72 22 internal internal JJ cord-319100-3gdawhfn 72 23 control control NN cord-319100-3gdawhfn 72 24 ( ( -LRB- cord-319100-3gdawhfn 72 25 XIPC XIPC NNP cord-319100-3gdawhfn 72 26 ) ) -RRB- cord-319100-3gdawhfn 72 27 assay assay NN cord-319100-3gdawhfn 72 28 ( ( -LRB- cord-319100-3gdawhfn 72 29 15 15 CD cord-319100-3gdawhfn 72 30 ) ) -RRB- cord-319100-3gdawhfn 72 31 . . . cord-319100-3gdawhfn 73 1 The the DT cord-319100-3gdawhfn 73 2 probes probe NNS cord-319100-3gdawhfn 73 3 for for IN cord-319100-3gdawhfn 73 4 the the DT cord-319100-3gdawhfn 73 5 coronavirus coronavirus NN cord-319100-3gdawhfn 73 6 assays assay NNS cord-319100-3gdawhfn 73 7 ( ( -LRB- cord-319100-3gdawhfn 73 8 E e NN cord-319100-3gdawhfn 73 9 / / SYM cord-319100-3gdawhfn 73 10 IP4 IP4 NNP cord-319100-3gdawhfn 73 11 ) ) -RRB- cord-319100-3gdawhfn 73 12 were be VBD cord-319100-3gdawhfn 73 13 both both DT cord-319100-3gdawhfn 73 14 labelled label VBN cord-319100-3gdawhfn 73 15 with with IN cord-319100-3gdawhfn 73 16 a a DT cord-319100-3gdawhfn 73 17 FAM FAM NNP cord-319100-3gdawhfn 73 18 reporter reporter NN cord-319100-3gdawhfn 73 19 and and CC cord-319100-3gdawhfn 73 20 black black JJ cord-319100-3gdawhfn 73 21 hole hole NN cord-319100-3gdawhfn 73 22 quencher quencher NN cord-319100-3gdawhfn 73 23 1 1 CD cord-319100-3gdawhfn 74 1 ( ( -LRB- cord-319100-3gdawhfn 74 2 Biosearch Biosearch NNP cord-319100-3gdawhfn 74 3 Technologies Technologies NNP cord-319100-3gdawhfn 74 4 ) ) -RRB- cord-319100-3gdawhfn 74 5 and and CC cord-319100-3gdawhfn 74 6 the the DT cord-319100-3gdawhfn 74 7 XIPC XIPC NNP cord-319100-3gdawhfn 74 8 probe probe NN cord-319100-3gdawhfn 74 9 with with IN cord-319100-3gdawhfn 74 10 a a DT cord-319100-3gdawhfn 74 11 Vic Vic NNP cord-319100-3gdawhfn 74 12 reporter reporter NN cord-319100-3gdawhfn 74 13 and and CC cord-319100-3gdawhfn 74 14 TAMRA TAMRA NNP cord-319100-3gdawhfn 74 15 quencher quencher NN cord-319100-3gdawhfn 74 16 ( ( -LRB- cord-319100-3gdawhfn 74 17 Life Life NNP cord-319100-3gdawhfn 74 18 Technologies Technologies NNPS cord-319100-3gdawhfn 74 19 ) ) -RRB- cord-319100-3gdawhfn 74 20 as as IN cord-319100-3gdawhfn 74 21 these these DT cord-319100-3gdawhfn 74 22 combinations combination NNS cord-319100-3gdawhfn 74 23 had have VBD cord-319100-3gdawhfn 74 24 been be VBN cord-319100-3gdawhfn 74 25 shown show VBN cord-319100-3gdawhfn 74 26 to to TO cord-319100-3gdawhfn 74 27 provide provide VB cord-319100-3gdawhfn 74 28 high high JJ cord-319100-3gdawhfn 74 29 analytical analytical JJ cord-319100-3gdawhfn 74 30 sensitivity sensitivity NN cord-319100-3gdawhfn 74 31 . . . cord-319100-3gdawhfn 75 1 A a DT cord-319100-3gdawhfn 75 2 commercial commercial JJ cord-319100-3gdawhfn 75 3 reverse reverse NN cord-319100-3gdawhfn 75 4 transcriptase transcriptase NN cord-319100-3gdawhfn 75 5 mastermix mastermix NNP cord-319100-3gdawhfn 75 6 ( ( -LRB- cord-319100-3gdawhfn 75 7 AgPath AgPath NNP cord-319100-3gdawhfn 75 8 - - HYPH cord-319100-3gdawhfn 75 9 ID ID NNP cord-319100-3gdawhfn 75 10 One one CD cord-319100-3gdawhfn 75 11 - - HYPH cord-319100-3gdawhfn 75 12 Step Step NNP cord-319100-3gdawhfn 75 13 RT RT NNP cord-319100-3gdawhfn 75 14 - - HYPH cord-319100-3gdawhfn 75 15 PCR PCR NNP cord-319100-3gdawhfn 75 16 kit kit NN cord-319100-3gdawhfn 75 17 -Life -Life NNP cord-319100-3gdawhfn 75 18 Technologies Technologies NNPS cord-319100-3gdawhfn 75 19 ) ) -RRB- cord-319100-3gdawhfn 75 20 was be VBD cord-319100-3gdawhfn 75 21 used use VBN cord-319100-3gdawhfn 75 22 and and CC cord-319100-3gdawhfn 75 23 run run VBN cord-319100-3gdawhfn 75 24 , , , cord-319100-3gdawhfn 75 25 with with IN cord-319100-3gdawhfn 75 26 slight slight JJ cord-319100-3gdawhfn 75 27 modifications modification NNS cord-319100-3gdawhfn 75 28 , , , cord-319100-3gdawhfn 75 29 under under IN cord-319100-3gdawhfn 75 30 the the DT cord-319100-3gdawhfn 75 31 standard standard JJ cord-319100-3gdawhfn 75 32 cycling cycling NN cord-319100-3gdawhfn 75 33 conditions condition NNS cord-319100-3gdawhfn 75 34 recommended recommend VBN cord-319100-3gdawhfn 75 35 by by IN cord-319100-3gdawhfn 75 36 the the DT cord-319100-3gdawhfn 75 37 manufacturer manufacturer NN cord-319100-3gdawhfn 75 38 , , , cord-319100-3gdawhfn 75 39 using use VBG cord-319100-3gdawhfn 75 40 an an DT cord-319100-3gdawhfn 75 41 ABI7500 ABI7500 NNP cord-319100-3gdawhfn 75 42 or or CC cord-319100-3gdawhfn 75 43 Quantstudio Quantstudio NNP cord-319100-3gdawhfn 75 44 5 5 CD cord-319100-3gdawhfn 76 1 ( ( -LRB- cord-319100-3gdawhfn 76 2 Thermofisher Thermofisher NNP cord-319100-3gdawhfn 76 3 Scientific Scientific NNP cord-319100-3gdawhfn 76 4 ) ) -RRB- cord-319100-3gdawhfn 76 5 thermocycler thermocycler NN cord-319100-3gdawhfn 76 6 . . . cord-319100-3gdawhfn 77 1 The the DT cord-319100-3gdawhfn 77 2 combined combined JJ cord-319100-3gdawhfn 77 3 annealing annealing NN cord-319100-3gdawhfn 77 4 and and CC cord-319100-3gdawhfn 77 5 extension extension NN cord-319100-3gdawhfn 77 6 temperatures temperature NNS cord-319100-3gdawhfn 77 7 were be VBD cord-319100-3gdawhfn 77 8 adjusted adjust VBN cord-319100-3gdawhfn 77 9 to to IN cord-319100-3gdawhfn 77 10 58 58 CD cord-319100-3gdawhfn 77 11 o o NN cord-319100-3gdawhfn 77 12 C c NN cord-319100-3gdawhfn 77 13 and and CC cord-319100-3gdawhfn 77 14 the the DT cord-319100-3gdawhfn 77 15 assay assay NN cord-319100-3gdawhfn 77 16 was be VBD cord-319100-3gdawhfn 77 17 run run VBN cord-319100-3gdawhfn 77 18 for for IN cord-319100-3gdawhfn 77 19 a a DT cord-319100-3gdawhfn 77 20 total total NN cord-319100-3gdawhfn 77 21 of of IN cord-319100-3gdawhfn 77 22 45 45 CD cord-319100-3gdawhfn 77 23 cycles cycle NNS cord-319100-3gdawhfn 77 24 . . . cord-319100-3gdawhfn 78 1 The the DT cord-319100-3gdawhfn 78 2 baseline baseline NN cord-319100-3gdawhfn 78 3 was be VBD cord-319100-3gdawhfn 78 4 manually manually RB cord-319100-3gdawhfn 78 5 set set VBN cord-319100-3gdawhfn 78 6 between between IN cord-319100-3gdawhfn 78 7 3 3 CD cord-319100-3gdawhfn 78 8 to to TO cord-319100-3gdawhfn 78 9 15 15 CD cord-319100-3gdawhfn 78 10 cycles cycle NNS cord-319100-3gdawhfn 78 11 and and CC cord-319100-3gdawhfn 78 12 the the DT cord-319100-3gdawhfn 78 13 threshold threshold NN cord-319100-3gdawhfn 78 14 at at IN cord-319100-3gdawhfn 78 15 0.05 0.05 CD cord-319100-3gdawhfn 78 16 . . . cord-319100-3gdawhfn 79 1 The the DT cord-319100-3gdawhfn 79 2 results result NNS cord-319100-3gdawhfn 79 3 were be VBD cord-319100-3gdawhfn 79 4 expressed express VBN cord-319100-3gdawhfn 79 5 as as IN cord-319100-3gdawhfn 79 6 cycle cycle NN cord-319100-3gdawhfn 79 7 - - HYPH cord-319100-3gdawhfn 79 8 threshold threshold NN cord-319100-3gdawhfn 79 9 ( ( -LRB- cord-319100-3gdawhfn 79 10 Ct Ct NNP cord-319100-3gdawhfn 79 11 ) ) -RRB- cord-319100-3gdawhfn 79 12 values value NNS cord-319100-3gdawhfn 79 13 , , , cord-319100-3gdawhfn 79 14 being be VBG cord-319100-3gdawhfn 79 15 the the DT cord-319100-3gdawhfn 79 16 number number NN cord-319100-3gdawhfn 79 17 of of IN cord-319100-3gdawhfn 79 18 PCR PCR NNP cord-319100-3gdawhfn 79 19 cycles cycle NNS cord-319100-3gdawhfn 79 20 at at IN cord-319100-3gdawhfn 79 21 which which WDT cord-319100-3gdawhfn 79 22 the the DT cord-319100-3gdawhfn 79 23 amplification amplification NN cord-319100-3gdawhfn 79 24 plot plot NN cord-319100-3gdawhfn 79 25 crossed cross VBD cord-319100-3gdawhfn 79 26 the the DT cord-319100-3gdawhfn 79 27 threshold threshold NN cord-319100-3gdawhfn 79 28 . . . cord-319100-3gdawhfn 80 1 For for IN cord-319100-3gdawhfn 80 2 comparative comparative JJ cord-319100-3gdawhfn 80 3 purposes purpose NNS cord-319100-3gdawhfn 80 4 a a DT cord-319100-3gdawhfn 80 5 selection selection NN cord-319100-3gdawhfn 80 6 of of IN cord-319100-3gdawhfn 80 7 samples sample NNS cord-319100-3gdawhfn 80 8 was be VBD cord-319100-3gdawhfn 80 9 also also RB cord-319100-3gdawhfn 80 10 tested test VBN cord-319100-3gdawhfn 80 11 using use VBG cord-319100-3gdawhfn 80 12 the the DT cord-319100-3gdawhfn 80 13 CDC CDC NNP cord-319100-3gdawhfn 80 14 designed design VBN cord-319100-3gdawhfn 80 15 assay assay NN cord-319100-3gdawhfn 80 16 ( ( -LRB- cord-319100-3gdawhfn 80 17 2019-nCoV_N2 2019-ncov_n2 CD cord-319100-3gdawhfn 80 18 ) ) -RRB- cord-319100-3gdawhfn 80 19 targeting target VBG cord-319100-3gdawhfn 80 20 the the DT cord-319100-3gdawhfn 80 21 N n CD cord-319100-3gdawhfn 80 22 gene gene NN cord-319100-3gdawhfn 80 23 , , , cord-319100-3gdawhfn 80 24 which which WDT cord-319100-3gdawhfn 80 25 was be VBD cord-319100-3gdawhfn 80 26 also also RB cord-319100-3gdawhfn 80 27 run run VBN cord-319100-3gdawhfn 80 28 under under IN cord-319100-3gdawhfn 80 29 the the DT cord-319100-3gdawhfn 80 30 published publish VBN cord-319100-3gdawhfn 80 31 conditions condition NNS cord-319100-3gdawhfn 80 32 ( ( -LRB- cord-319100-3gdawhfn 80 33 16 16 CD cord-319100-3gdawhfn 80 34 ) ) -RRB- cord-319100-3gdawhfn 80 35 with with IN cord-319100-3gdawhfn 80 36 the the DT cord-319100-3gdawhfn 80 37 same same JJ cord-319100-3gdawhfn 80 38 commercial commercial JJ cord-319100-3gdawhfn 80 39 mastermix mastermix NN cord-319100-3gdawhfn 80 40 . . . cord-319100-3gdawhfn 81 1 Published publish VBN cord-319100-3gdawhfn 81 2 primer primer NN cord-319100-3gdawhfn 81 3 and and CC cord-319100-3gdawhfn 81 4 probe probe NN cord-319100-3gdawhfn 81 5 sets set NNS cord-319100-3gdawhfn 81 6 were be VBD cord-319100-3gdawhfn 81 7 used use VBN cord-319100-3gdawhfn 81 8 for for IN cord-319100-3gdawhfn 81 9 the the DT cord-319100-3gdawhfn 81 10 pan pan JJ cord-319100-3gdawhfn 81 11 - - JJ cord-319100-3gdawhfn 81 12 reactive reactive JJ cord-319100-3gdawhfn 81 13 Type type NN cord-319100-3gdawhfn 81 14 A A NNP cord-319100-3gdawhfn 81 15 influenza influenza NN cord-319100-3gdawhfn 81 16 qRT qRT NNP cord-319100-3gdawhfn 81 17 - - HYPH cord-319100-3gdawhfn 81 18 PCR PCR NNP cord-319100-3gdawhfn 81 19 ( ( -LRB- cord-319100-3gdawhfn 81 20 17 17 CD cord-319100-3gdawhfn 81 21 ) ) -RRB- cord-319100-3gdawhfn 81 22 , , , cord-319100-3gdawhfn 81 23 the the DT cord-319100-3gdawhfn 81 24 BHV-1 BHV-1 NNP cord-319100-3gdawhfn 81 25 qPCR qpcr XX cord-319100-3gdawhfn 81 26 ( ( -LRB- cord-319100-3gdawhfn 81 27 18 18 CD cord-319100-3gdawhfn 81 28 ) ) -RRB- cord-319100-3gdawhfn 81 29 and and CC cord-319100-3gdawhfn 81 30 the the DT cord-319100-3gdawhfn 81 31 pan pan JJ cord-319100-3gdawhfn 81 32 - - HYPH cord-319100-3gdawhfn 81 33 pestvirus pestvirus NNP cord-319100-3gdawhfn 81 34 qRT qRT NNP cord-319100-3gdawhfn 81 35 - - HYPH cord-319100-3gdawhfn 81 36 PCR PCR NNP cord-319100-3gdawhfn 81 37 ( ( -LRB- cord-319100-3gdawhfn 81 38 19 19 CD cord-319100-3gdawhfn 81 39 ) ) -RRB- cord-319100-3gdawhfn 81 40 assays assay NNS cord-319100-3gdawhfn 81 41 , , , cord-319100-3gdawhfn 81 42 each each DT cord-319100-3gdawhfn 81 43 in in IN cord-319100-3gdawhfn 81 44 a a DT cord-319100-3gdawhfn 81 45 duplex duplex NN cord-319100-3gdawhfn 81 46 format format NN cord-319100-3gdawhfn 81 47 with with IN cord-319100-3gdawhfn 81 48 the the DT cord-319100-3gdawhfn 81 49 XIPC XIPC NNP cord-319100-3gdawhfn 81 50 assay assay NN cord-319100-3gdawhfn 81 51 . . . cord-319100-3gdawhfn 82 1 Each each DT cord-319100-3gdawhfn 82 2 assay assay NN cord-319100-3gdawhfn 82 3 utilised utilise VBD cord-319100-3gdawhfn 82 4 the the DT cord-319100-3gdawhfn 82 5 AgPath AgPath NNP cord-319100-3gdawhfn 82 6 - - HYPH cord-319100-3gdawhfn 82 7 ID ID NNP cord-319100-3gdawhfn 82 8 One one CD cord-319100-3gdawhfn 82 9 - - HYPH cord-319100-3gdawhfn 82 10 Step Step NNP cord-319100-3gdawhfn 82 11 RT RT NNP cord-319100-3gdawhfn 82 12 - - HYPH cord-319100-3gdawhfn 82 13 PCR PCR NNP cord-319100-3gdawhfn 82 14 mastermix mastermix NN cord-319100-3gdawhfn 82 15 and and CC cord-319100-3gdawhfn 82 16 were be VBD cord-319100-3gdawhfn 82 17 run run VBN cord-319100-3gdawhfn 82 18 for for IN cord-319100-3gdawhfn 82 19 45 45 CD cord-319100-3gdawhfn 82 20 cycles cycle NNS cord-319100-3gdawhfn 82 21 under under IN cord-319100-3gdawhfn 82 22 the the DT cord-319100-3gdawhfn 82 23 standard standard JJ cord-319100-3gdawhfn 82 24 cycling cycling NN cord-319100-3gdawhfn 82 25 conditions condition NNS cord-319100-3gdawhfn 82 26 recommended recommend VBN cord-319100-3gdawhfn 82 27 by by IN cord-319100-3gdawhfn 82 28 the the DT cord-319100-3gdawhfn 82 29 manufacturer manufacturer NN cord-319100-3gdawhfn 82 30 , , , cord-319100-3gdawhfn 82 31 with with IN cord-319100-3gdawhfn 82 32 the the DT cord-319100-3gdawhfn 82 33 baseline baseline NN cord-319100-3gdawhfn 82 34 set set NN cord-319100-3gdawhfn 82 35 automatically automatically RB cord-319100-3gdawhfn 82 36 and and CC cord-319100-3gdawhfn 82 37 the the DT cord-319100-3gdawhfn 82 38 threshold threshold NN cord-319100-3gdawhfn 82 39 at at IN cord-319100-3gdawhfn 82 40 0.05 0.05 CD cord-319100-3gdawhfn 82 41 . . . cord-319100-3gdawhfn 83 1 All all DT cord-319100-3gdawhfn 83 2 assays assay NNS cord-319100-3gdawhfn 83 3 included include VBD cord-319100-3gdawhfn 83 4 at at IN cord-319100-3gdawhfn 83 5 least least JJS cord-319100-3gdawhfn 83 6 2 2 CD cord-319100-3gdawhfn 83 7 positive positive JJ cord-319100-3gdawhfn 83 8 controls control NNS cord-319100-3gdawhfn 83 9 , , , cord-319100-3gdawhfn 83 10 one one CD cord-319100-3gdawhfn 83 11 negative negative JJ cord-319100-3gdawhfn 83 12 control control NN cord-319100-3gdawhfn 83 13 ( ( -LRB- cord-319100-3gdawhfn 83 14 tRNA tRNA NNP cord-319100-3gdawhfn 83 15 ) ) -RRB- cord-319100-3gdawhfn 83 16 and and CC cord-319100-3gdawhfn 83 17 a a DT cord-319100-3gdawhfn 83 18 ' ' `` cord-319100-3gdawhfn 83 19 no no DT cord-319100-3gdawhfn 83 20 template template NN cord-319100-3gdawhfn 83 21 ' ' '' cord-319100-3gdawhfn 83 22 control control NN cord-319100-3gdawhfn 83 23 ( ( -LRB- cord-319100-3gdawhfn 83 24 NTC NTC NNP cord-319100-3gdawhfn 83 25 -nuclease -nuclease NNP cord-319100-3gdawhfn 83 26 free free JJ cord-319100-3gdawhfn 83 27 water water NN cord-319100-3gdawhfn 83 28 ) ) -RRB- cord-319100-3gdawhfn 83 29 . . . cord-319100-3gdawhfn 84 1 The the DT cord-319100-3gdawhfn 84 2 XIPC XIPC NNP cord-319100-3gdawhfn 84 3 RNA RNA NNP cord-319100-3gdawhfn 84 4 ( ( -LRB- cord-319100-3gdawhfn 84 5 approximately approximately RB cord-319100-3gdawhfn 84 6 80 80 CD cord-319100-3gdawhfn 84 7 copies copy NNS cord-319100-3gdawhfn 84 8 / / SYM cord-319100-3gdawhfn 84 9 uL ul NN cord-319100-3gdawhfn 84 10 ) ) -RRB- cord-319100-3gdawhfn 84 11 was be VBD cord-319100-3gdawhfn 84 12 included include VBN cord-319100-3gdawhfn 84 13 in in IN cord-319100-3gdawhfn 84 14 the the DT cord-319100-3gdawhfn 84 15 sample sample NN cord-319100-3gdawhfn 84 16 lysis lysis NN cord-319100-3gdawhfn 84 17 buffer buffer NN cord-319100-3gdawhfn 84 18 prior prior RB cord-319100-3gdawhfn 84 19 to to IN cord-319100-3gdawhfn 84 20 the the DT cord-319100-3gdawhfn 84 21 extraction extraction NN cord-319100-3gdawhfn 84 22 of of IN cord-319100-3gdawhfn 84 23 nucleic nucleic JJ cord-319100-3gdawhfn 84 24 acid acid NN cord-319100-3gdawhfn 84 25 from from IN cord-319100-3gdawhfn 84 26 all all DT cord-319100-3gdawhfn 84 27 test test NN cord-319100-3gdawhfn 84 28 and and CC cord-319100-3gdawhfn 84 29 control control NN cord-319100-3gdawhfn 84 30 samples sample NNS cord-319100-3gdawhfn 84 31 except except IN cord-319100-3gdawhfn 84 32 for for IN cord-319100-3gdawhfn 84 33 the the DT cord-319100-3gdawhfn 84 34 NTC NTC NNP cord-319100-3gdawhfn 84 35 wells well NNS cord-319100-3gdawhfn 84 36 . . . cord-319100-3gdawhfn 85 1 The the DT cord-319100-3gdawhfn 85 2 results result NNS cord-319100-3gdawhfn 85 3 of of IN cord-319100-3gdawhfn 85 4 these these DT cord-319100-3gdawhfn 85 5 investigations investigation NNS cord-319100-3gdawhfn 85 6 are be VBP cord-319100-3gdawhfn 85 7 documented document VBN cord-319100-3gdawhfn 85 8 in in IN cord-319100-3gdawhfn 85 9 Tables table NNS cord-319100-3gdawhfn 85 10 1 1 CD cord-319100-3gdawhfn 85 11 - - SYM cord-319100-3gdawhfn 85 12 9 9 CD cord-319100-3gdawhfn 85 13 . . . cord-319100-3gdawhfn 86 1 The the DT cord-319100-3gdawhfn 86 2 preliminary preliminary JJ cord-319100-3gdawhfn 86 3 investigation investigation NN cord-319100-3gdawhfn 86 4 ( ( -LRB- cord-319100-3gdawhfn 86 5 Table Table NNP cord-319100-3gdawhfn 86 6 1 1 CD cord-319100-3gdawhfn 86 7 ) ) -RRB- cord-319100-3gdawhfn 86 8 showed show VBD cord-319100-3gdawhfn 86 9 that that IN cord-319100-3gdawhfn 86 10 no no DT cord-319100-3gdawhfn 86 11 SARS SARS NNP cord-319100-3gdawhfn 86 12 - - HYPH cord-319100-3gdawhfn 86 13 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 86 14 RNA RNA NNP cord-319100-3gdawhfn 86 15 was be VBD cord-319100-3gdawhfn 86 16 detected detect VBN cord-319100-3gdawhfn 86 17 after after IN cord-319100-3gdawhfn 86 18 dilution dilution NN cord-319100-3gdawhfn 86 19 inVTM-2 inVTM-2 NNP cord-319100-3gdawhfn 86 20 or or CC cord-319100-3gdawhfn 86 21 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 86 22 . . . cord-319100-3gdawhfn 87 1 In in IN cord-319100-3gdawhfn 87 2 contrast contrast NN cord-319100-3gdawhfn 87 3 , , , cord-319100-3gdawhfn 87 4 the the DT cord-319100-3gdawhfn 87 5 results result NNS cord-319100-3gdawhfn 87 6 for for IN cord-319100-3gdawhfn 87 7 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 87 8 were be VBD cord-319100-3gdawhfn 87 9 at at IN cord-319100-3gdawhfn 87 10 the the DT cord-319100-3gdawhfn 87 11 expected expect VBN cord-319100-3gdawhfn 87 12 levels level NNS cord-319100-3gdawhfn 87 13 and and CC cord-319100-3gdawhfn 87 14 those those DT cord-319100-3gdawhfn 87 15 for for IN cord-319100-3gdawhfn 87 16 the the DT cord-319100-3gdawhfn 87 17 XIPC XIPC NNP cord-319100-3gdawhfn 87 18 were be VBD cord-319100-3gdawhfn 87 19 highly highly RB cord-319100-3gdawhfn 87 20 reproducible reproducible JJ cord-319100-3gdawhfn 87 21 for for IN cord-319100-3gdawhfn 87 22 each each DT cord-319100-3gdawhfn 87 23 sample sample NN cord-319100-3gdawhfn 87 24 , , , cord-319100-3gdawhfn 87 25 indicating indicate VBG cord-319100-3gdawhfn 87 26 that that IN cord-319100-3gdawhfn 87 27 there there EX cord-319100-3gdawhfn 87 28 had have VBD cord-319100-3gdawhfn 87 29 been be VBN cord-319100-3gdawhfn 87 30 efficient efficient JJ cord-319100-3gdawhfn 87 31 nucleic nucleic JJ cord-319100-3gdawhfn 87 32 acid acid NN cord-319100-3gdawhfn 87 33 extraction extraction NN cord-319100-3gdawhfn 87 34 and and CC cord-319100-3gdawhfn 87 35 that that IN cord-319100-3gdawhfn 87 36 there there EX cord-319100-3gdawhfn 87 37 were be VBD cord-319100-3gdawhfn 87 38 no no DT cord-319100-3gdawhfn 87 39 inhibitors inhibitor NNS cord-319100-3gdawhfn 87 40 of of IN cord-319100-3gdawhfn 87 41 the the DT cord-319100-3gdawhfn 87 42 qRT qRT NNP cord-319100-3gdawhfn 87 43 - - HYPH cord-319100-3gdawhfn 87 44 PCR PCR NNP cord-319100-3gdawhfn 87 45 in in IN cord-319100-3gdawhfn 87 46 the the DT cord-319100-3gdawhfn 87 47 samples sample NNS cord-319100-3gdawhfn 87 48 . . . cord-319100-3gdawhfn 88 1 Table table NN cord-319100-3gdawhfn 88 2 1 1 CD cord-319100-3gdawhfn 89 1 -Preliminary -preliminary JJ cord-319100-3gdawhfn 89 2 comparison comparison NN cord-319100-3gdawhfn 89 3 of of IN cord-319100-3gdawhfn 89 4 SARS SARS NNP cord-319100-3gdawhfn 89 5 - - HYPH cord-319100-3gdawhfn 89 6 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 89 7 qRT qRT NNP cord-319100-3gdawhfn 89 8 - - HYPH cord-319100-3gdawhfn 89 9 PCR PCR NNP cord-319100-3gdawhfn 89 10 Ct Ct NNP cord-319100-3gdawhfn 89 11 values value NNS cord-319100-3gdawhfn 89 12 when when WRB cord-319100-3gdawhfn 89 13 testing test VBG cord-319100-3gdawhfn 89 14 SARS SARS NNP cord-319100-3gdawhfn 89 15 - - HYPH cord-319100-3gdawhfn 89 16 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 89 17 purified purify VBN cord-319100-3gdawhfn 89 18 RNA RNA NNP cord-319100-3gdawhfn 89 19 diluted dilute VBN cord-319100-3gdawhfn 89 20 in in IN cord-319100-3gdawhfn 89 21 different different JJ cord-319100-3gdawhfn 89 22 viral viral JJ cord-319100-3gdawhfn 89 23 transport transport NN cord-319100-3gdawhfn 89 24 media medium NNS cord-319100-3gdawhfn 89 25 . . . cord-319100-3gdawhfn 90 1 To to TO cord-319100-3gdawhfn 90 2 confirm confirm VB cord-319100-3gdawhfn 90 3 the the DT cord-319100-3gdawhfn 90 4 initial initial JJ cord-319100-3gdawhfn 90 5 results result NNS cord-319100-3gdawhfn 90 6 , , , cord-319100-3gdawhfn 90 7 the the DT cord-319100-3gdawhfn 90 8 experiment experiment NN cord-319100-3gdawhfn 90 9 was be VBD cord-319100-3gdawhfn 90 10 repeated repeat VBN cord-319100-3gdawhfn 90 11 ( ( -LRB- cord-319100-3gdawhfn 90 12 Table table NN cord-319100-3gdawhfn 90 13 2 2 CD cord-319100-3gdawhfn 90 14 , , , cord-319100-3gdawhfn 90 15 Samples sample NNS cord-319100-3gdawhfn 90 16 4 4 CD cord-319100-3gdawhfn 90 17 - - SYM cord-319100-3gdawhfn 90 18 9 9 CD cord-319100-3gdawhfn 90 19 ) ) -RRB- cord-319100-3gdawhfn 90 20 , , , cord-319100-3gdawhfn 90 21 with with IN cord-319100-3gdawhfn 90 22 the the DT cord-319100-3gdawhfn 90 23 inclusion inclusion NN cord-319100-3gdawhfn 90 24 of of IN cord-319100-3gdawhfn 90 25 both both CC cord-319100-3gdawhfn 90 26 RNA RNA NNP cord-319100-3gdawhfn 90 27 and and CC cord-319100-3gdawhfn 90 28 presumptively presumptively RB cord-319100-3gdawhfn 90 29 whole whole JJ cord-319100-3gdawhfn 90 30 virus virus NN cord-319100-3gdawhfn 90 31 , , , cord-319100-3gdawhfn 90 32 as as RB cord-319100-3gdawhfn 90 33 well well RB cord-319100-3gdawhfn 90 34 as as IN cord-319100-3gdawhfn 90 35 PBS PBS NNP cord-319100-3gdawhfn 90 36 and and CC cord-319100-3gdawhfn 90 37 additional additional JJ cord-319100-3gdawhfn 90 38 VTM VTM NNP cord-319100-3gdawhfn 90 39 solutions solution NNS cord-319100-3gdawhfn 90 40 . . . cord-319100-3gdawhfn 91 1 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 91 2 and and CC cord-319100-3gdawhfn 91 3 PBS PBS NNP cord-319100-3gdawhfn 91 4 gave give VBD cord-319100-3gdawhfn 91 5 very very RB cord-319100-3gdawhfn 91 6 similar similar JJ cord-319100-3gdawhfn 91 7 results result NNS cord-319100-3gdawhfn 91 8 for for IN cord-319100-3gdawhfn 91 9 the the DT cord-319100-3gdawhfn 91 10 RNA RNA NNP cord-319100-3gdawhfn 91 11 samples sample NNS cord-319100-3gdawhfn 91 12 . . . cord-319100-3gdawhfn 92 1 Again again RB cord-319100-3gdawhfn 92 2 , , , cord-319100-3gdawhfn 92 3 no no DT cord-319100-3gdawhfn 92 4 SARS SARS NNP cord-319100-3gdawhfn 92 5 - - HYPH cord-319100-3gdawhfn 92 6 Cov-2 Cov-2 NNP cord-319100-3gdawhfn 92 7 RNA RNA NNP cord-319100-3gdawhfn 92 8 was be VBD cord-319100-3gdawhfn 92 9 detected detect VBN cord-319100-3gdawhfn 92 10 in in IN cord-319100-3gdawhfn 92 11 the the DT cord-319100-3gdawhfn 92 12 dilutions dilution NNS cord-319100-3gdawhfn 92 13 prepared prepare VBN cord-319100-3gdawhfn 92 14 in in IN cord-319100-3gdawhfn 92 15 VTMs VTMs NNP cord-319100-3gdawhfn 92 16 2 2 CD cord-319100-3gdawhfn 92 17 - - SYM cord-319100-3gdawhfn 92 18 4 4 CD cord-319100-3gdawhfn 92 19 . . . cord-319100-3gdawhfn 93 1 Consequently consequently RB cord-319100-3gdawhfn 93 2 , , , cord-319100-3gdawhfn 93 3 a a DT cord-319100-3gdawhfn 93 4 further further JJ cord-319100-3gdawhfn 93 5 set set NN cord-319100-3gdawhfn 93 6 of of IN cord-319100-3gdawhfn 93 7 dilutions dilution NNS cord-319100-3gdawhfn 93 8 ( ( -LRB- cord-319100-3gdawhfn 93 9 Table table NN cord-319100-3gdawhfn 93 10 2 2 CD cord-319100-3gdawhfn 93 11 , , , cord-319100-3gdawhfn 93 12 Samples sample NNS cord-319100-3gdawhfn 93 13 1 1 CD cord-319100-3gdawhfn 93 14 - - SYM cord-319100-3gdawhfn 93 15 3 3 CD cord-319100-3gdawhfn 93 16 ) ) -RRB- cord-319100-3gdawhfn 93 17 were be VBD cord-319100-3gdawhfn 93 18 prepared prepare VBN cord-319100-3gdawhfn 93 19 in in IN cord-319100-3gdawhfn 93 20 each each DT cord-319100-3gdawhfn 93 21 solution solution NN cord-319100-3gdawhfn 93 22 to to TO cord-319100-3gdawhfn 93 23 test test VB cord-319100-3gdawhfn 93 24 whether whether IN cord-319100-3gdawhfn 93 25 there there EX cord-319100-3gdawhfn 93 26 was be VBD cord-319100-3gdawhfn 93 27 any any DT cord-319100-3gdawhfn 93 28 adverse adverse JJ cord-319100-3gdawhfn 93 29 effect effect NN cord-319100-3gdawhfn 93 30 on on IN cord-319100-3gdawhfn 93 31 higher high JJR cord-319100-3gdawhfn 93 32 concentrations concentration NNS cord-319100-3gdawhfn 93 33 of of IN cord-319100-3gdawhfn 93 34 RNA RNA NNP cord-319100-3gdawhfn 93 35 . . . cord-319100-3gdawhfn 94 1 SARS SARS NNP cord-319100-3gdawhfn 95 1 -Cov-2 -Cov-2 : cord-319100-3gdawhfn 95 2 RNA RNA NNP cord-319100-3gdawhfn 95 3 was be VBD cord-319100-3gdawhfn 95 4 not not RB cord-319100-3gdawhfn 95 5 detected detect VBN cord-319100-3gdawhfn 95 6 in in IN cord-319100-3gdawhfn 95 7 any any DT cord-319100-3gdawhfn 95 8 dilution dilution NN cord-319100-3gdawhfn 95 9 prepared prepare VBN cord-319100-3gdawhfn 95 10 in in IN cord-319100-3gdawhfn 95 11 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 95 12 and and CC cord-319100-3gdawhfn 95 13 very very RB cord-319100-3gdawhfn 95 14 weak weak JJ cord-319100-3gdawhfn 95 15 reactivity reactivity NN cord-319100-3gdawhfn 95 16 ( ( -LRB- cord-319100-3gdawhfn 95 17 approaching approach VBG cord-319100-3gdawhfn 95 18 the the DT cord-319100-3gdawhfn 95 19 assays assay NNS cord-319100-3gdawhfn 95 20 limit limit NN cord-319100-3gdawhfn 95 21 of of IN cord-319100-3gdawhfn 95 22 detection detection NN cord-319100-3gdawhfn 95 23 ) ) -RRB- cord-319100-3gdawhfn 95 24 was be VBD cord-319100-3gdawhfn 95 25 observed observe VBN cord-319100-3gdawhfn 95 26 for for IN cord-319100-3gdawhfn 95 27 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 95 28 and and CC cord-319100-3gdawhfn 95 29 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 95 30 in in IN cord-319100-3gdawhfn 95 31 the the DT cord-319100-3gdawhfn 95 32 sample sample NN cord-319100-3gdawhfn 95 33 with with IN cord-319100-3gdawhfn 95 34 the the DT cord-319100-3gdawhfn 95 35 highest high JJS cord-319100-3gdawhfn 95 36 concentration concentration NN cord-319100-3gdawhfn 95 37 of of IN cord-319100-3gdawhfn 95 38 RNA RNA NNP cord-319100-3gdawhfn 95 39 . . . cord-319100-3gdawhfn 96 1 In in IN cord-319100-3gdawhfn 96 2 contrast contrast NN cord-319100-3gdawhfn 96 3 , , , cord-319100-3gdawhfn 96 4 samples sample NNS cord-319100-3gdawhfn 96 5 diluted dilute VBN cord-319100-3gdawhfn 96 6 in in IN cord-319100-3gdawhfn 96 7 PBS PBS NNP cord-319100-3gdawhfn 96 8 and and CC cord-319100-3gdawhfn 96 9 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 96 10 gave give VBD cord-319100-3gdawhfn 96 11 almost almost RB cord-319100-3gdawhfn 96 12 identical identical JJ cord-319100-3gdawhfn 96 13 results result NNS cord-319100-3gdawhfn 96 14 , , , cord-319100-3gdawhfn 96 15 with with IN cord-319100-3gdawhfn 96 16 a a DT cord-319100-3gdawhfn 96 17 Ct Ct NNP cord-319100-3gdawhfn 96 18 value value NN cord-319100-3gdawhfn 96 19 of of IN cord-319100-3gdawhfn 96 20 approximately approximately RB cord-319100-3gdawhfn 96 21 21 21 CD cord-319100-3gdawhfn 96 22 for for IN cord-319100-3gdawhfn 96 23 the the DT cord-319100-3gdawhfn 96 24 highest high JJS cord-319100-3gdawhfn 96 25 RNA RNA NNP cord-319100-3gdawhfn 96 26 concentration concentration NN cord-319100-3gdawhfn 96 27 . . . cord-319100-3gdawhfn 97 1 This this DT cord-319100-3gdawhfn 97 2 difference difference NN cord-319100-3gdawhfn 97 3 between between IN cord-319100-3gdawhfn 97 4 the the DT cord-319100-3gdawhfn 97 5 PBS PBS NNP cord-319100-3gdawhfn 97 6 / / SYM cord-319100-3gdawhfn 97 7 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 97 8 result result NN cord-319100-3gdawhfn 97 9 and and CC cord-319100-3gdawhfn 97 10 the the DT cord-319100-3gdawhfn 97 11 results result NNS cord-319100-3gdawhfn 97 12 for for IN cord-319100-3gdawhfn 97 13 the the DT cord-319100-3gdawhfn 97 14 other other JJ cord-319100-3gdawhfn 97 15 VTMs vtms NN cord-319100-3gdawhfn 97 16 represents represent VBZ cord-319100-3gdawhfn 97 17 a a DT cord-319100-3gdawhfn 97 18 reduction reduction NN cord-319100-3gdawhfn 97 19 in in IN cord-319100-3gdawhfn 97 20 analytical analytical JJ cord-319100-3gdawhfn 97 21 sensitivity sensitivity NN cord-319100-3gdawhfn 97 22 of of IN cord-319100-3gdawhfn 97 23 approximately approximately RB cord-319100-3gdawhfn 97 24 6 6 CD cord-319100-3gdawhfn 97 25 log10 log10 NNS cord-319100-3gdawhfn 97 26 for for IN cord-319100-3gdawhfn 97 27 the the DT cord-319100-3gdawhfn 97 28 detection detection NN cord-319100-3gdawhfn 97 29 of of IN cord-319100-3gdawhfn 97 30 free free JJ cord-319100-3gdawhfn 97 31 SARS SARS NNP cord-319100-3gdawhfn 97 32 - - HYPH cord-319100-3gdawhfn 97 33 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 97 34 RNA RNA NNP cord-319100-3gdawhfn 97 35 . . . cord-319100-3gdawhfn 98 1 The the DT cord-319100-3gdawhfn 98 2 results result NNS cord-319100-3gdawhfn 98 3 for for IN cord-319100-3gdawhfn 98 4 the the DT cord-319100-3gdawhfn 98 5 XIPC XIPC NNP cord-319100-3gdawhfn 98 6 were be VBD cord-319100-3gdawhfn 98 7 again again RB cord-319100-3gdawhfn 98 8 highly highly RB cord-319100-3gdawhfn 98 9 reproducible reproducible JJ cord-319100-3gdawhfn 98 10 and and CC cord-319100-3gdawhfn 98 11 similar similar JJ cord-319100-3gdawhfn 98 12 for for IN cord-319100-3gdawhfn 98 13 each each DT cord-319100-3gdawhfn 98 14 dilution dilution NN cord-319100-3gdawhfn 98 15 in in IN cord-319100-3gdawhfn 98 16 each each DT cord-319100-3gdawhfn 98 17 solution solution NN cord-319100-3gdawhfn 98 18 , , , cord-319100-3gdawhfn 98 19 confirming confirm VBG cord-319100-3gdawhfn 98 20 the the DT cord-319100-3gdawhfn 98 21 high high JJ cord-319100-3gdawhfn 98 22 efficiency efficiency NN cord-319100-3gdawhfn 98 23 of of IN cord-319100-3gdawhfn 98 24 RNA RNA NNP cord-319100-3gdawhfn 98 25 extraction extraction NN cord-319100-3gdawhfn 98 26 and and CC cord-319100-3gdawhfn 98 27 no no DT cord-319100-3gdawhfn 98 28 apparent apparent JJ cord-319100-3gdawhfn 98 29 impact impact NN cord-319100-3gdawhfn 98 30 of of IN cord-319100-3gdawhfn 98 31 PCR PCR NNP cord-319100-3gdawhfn 98 32 inhibitors inhibitor NNS cord-319100-3gdawhfn 98 33 . . . cord-319100-3gdawhfn 98 34 _SP cord-319100-3gdawhfn 99 1 Testing testing NN cord-319100-3gdawhfn 99 2 was be VBD cord-319100-3gdawhfn 99 3 then then RB cord-319100-3gdawhfn 99 4 undertaken undertake VBN cord-319100-3gdawhfn 99 5 to to TO cord-319100-3gdawhfn 99 6 determine determine VB cord-319100-3gdawhfn 99 7 whether whether IN cord-319100-3gdawhfn 99 8 there there EX cord-319100-3gdawhfn 99 9 might may MD cord-319100-3gdawhfn 99 10 be be VB cord-319100-3gdawhfn 99 11 a a DT cord-319100-3gdawhfn 99 12 reduction reduction NN cord-319100-3gdawhfn 99 13 in in IN cord-319100-3gdawhfn 99 14 sensitivity sensitivity NN cord-319100-3gdawhfn 99 15 when when WRB cord-319100-3gdawhfn 99 16 testing test VBG cord-319100-3gdawhfn 99 17 a a DT cord-319100-3gdawhfn 99 18 sample sample NN cord-319100-3gdawhfn 99 19 that that WDT cord-319100-3gdawhfn 99 20 presumptively presumptively RB cord-319100-3gdawhfn 99 21 contains contain VBZ cord-319100-3gdawhfn 99 22 high high JJ cord-319100-3gdawhfn 99 23 quality quality NN cord-319100-3gdawhfn 99 24 intact intact JJ cord-319100-3gdawhfn 99 25 virions virion NNS cord-319100-3gdawhfn 99 26 . . . cord-319100-3gdawhfn 100 1 In in IN cord-319100-3gdawhfn 100 2 this this DT cord-319100-3gdawhfn 100 3 instance instance NN cord-319100-3gdawhfn 100 4 , , , cord-319100-3gdawhfn 100 5 the the DT cord-319100-3gdawhfn 100 6 results result NNS cord-319100-3gdawhfn 100 7 for for IN cord-319100-3gdawhfn 100 8 the the DT cord-319100-3gdawhfn 100 9 dilutions dilution NNS cord-319100-3gdawhfn 100 10 of of IN cord-319100-3gdawhfn 100 11 virus virus NN cord-319100-3gdawhfn 100 12 ( ( -LRB- cord-319100-3gdawhfn 100 13 Table table NN cord-319100-3gdawhfn 100 14 3 3 CD cord-319100-3gdawhfn 100 15 ) ) -RRB- cord-319100-3gdawhfn 100 16 were be VBD cord-319100-3gdawhfn 100 17 similar similar JJ cord-319100-3gdawhfn 100 18 for for IN cord-319100-3gdawhfn 100 19 each each DT cord-319100-3gdawhfn 100 20 VTM VTM NNP cord-319100-3gdawhfn 100 21 and and CC cord-319100-3gdawhfn 100 22 the the DT cord-319100-3gdawhfn 100 23 PBS PBS NNP cord-319100-3gdawhfn 100 24 , , , cord-319100-3gdawhfn 100 25 as as IN cord-319100-3gdawhfn 100 26 were be VBD cord-319100-3gdawhfn 100 27 the the DT cord-319100-3gdawhfn 100 28 results result NNS cord-319100-3gdawhfn 100 29 for for IN cord-319100-3gdawhfn 100 30 the the DT cord-319100-3gdawhfn 100 31 XIPC XIPC NNP cord-319100-3gdawhfn 100 32 . . . cord-319100-3gdawhfn 101 1 Similar similar JJ cord-319100-3gdawhfn 101 2 results result NNS cord-319100-3gdawhfn 101 3 for for IN cord-319100-3gdawhfn 101 4 both both DT cord-319100-3gdawhfn 101 5 SARS SARS NNP cord-319100-3gdawhfn 101 6 - - HYPH cord-319100-3gdawhfn 101 7 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 101 8 purified purify VBN cord-319100-3gdawhfn 101 9 RNA RNA NNP cord-319100-3gdawhfn 101 10 and and CC cord-319100-3gdawhfn 101 11 the the DT cord-319100-3gdawhfn 101 12 patient patient JJ cord-319100-3gdawhfn 101 13 sample sample NN cord-319100-3gdawhfn 101 14 were be VBD cord-319100-3gdawhfn 101 15 obtained obtain VBN cord-319100-3gdawhfn 101 16 in in IN cord-319100-3gdawhfn 101 17 the the DT cord-319100-3gdawhfn 101 18 CDC CDC NNP cord-319100-3gdawhfn 101 19 assay assay NN cord-319100-3gdawhfn 101 20 ( ( -LRB- cord-319100-3gdawhfn 101 21 2019-nCoV_N2 2019-ncov_n2 CD cord-319100-3gdawhfn 101 22 ) ) -RRB- cord-319100-3gdawhfn 101 23 targeting target VBG cord-319100-3gdawhfn 101 24 the the DT cord-319100-3gdawhfn 101 25 N n CD cord-319100-3gdawhfn 101 26 gene gene NN cord-319100-3gdawhfn 101 27 ( ( -LRB- cord-319100-3gdawhfn 101 28 results result NNS cord-319100-3gdawhfn 101 29 not not RB cord-319100-3gdawhfn 101 30 included include VBN cord-319100-3gdawhfn 101 31 ) ) -RRB- cord-319100-3gdawhfn 101 32 . . . cord-319100-3gdawhfn 102 1 Testing testing NN cord-319100-3gdawhfn 102 2 of of IN cord-319100-3gdawhfn 102 3 the the DT cord-319100-3gdawhfn 102 4 samples sample NNS cord-319100-3gdawhfn 102 5 of of IN cord-319100-3gdawhfn 102 6 VTM-1 VTM-1 NNS cord-319100-3gdawhfn 102 7 to to TO cord-319100-3gdawhfn 102 8 which which WDT cord-319100-3gdawhfn 102 9 swabs swab NNS cord-319100-3gdawhfn 102 10 from from IN cord-319100-3gdawhfn 102 11 SARS SARS NNP cord-319100-3gdawhfn 102 12 - - HYPH cord-319100-3gdawhfn 102 13 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 102 14 positive positive JJ cord-319100-3gdawhfn 102 15 patients patient NNS cord-319100-3gdawhfn 102 16 had have VBD cord-319100-3gdawhfn 102 17 been be VBN cord-319100-3gdawhfn 102 18 added add VBN cord-319100-3gdawhfn 102 19 and and CC cord-319100-3gdawhfn 102 20 stored store VBN cord-319100-3gdawhfn 102 21 at at IN cord-319100-3gdawhfn 102 22 4 4 CD cord-319100-3gdawhfn 102 23 o o NN cord-319100-3gdawhfn 102 24 C c NN cord-319100-3gdawhfn 102 25 for for IN cord-319100-3gdawhfn 102 26 46 46 CD cord-319100-3gdawhfn 102 27 - - SYM cord-319100-3gdawhfn 102 28 49 49 CD cord-319100-3gdawhfn 102 29 days day NNS cord-319100-3gdawhfn 102 30 gave give VBD cord-319100-3gdawhfn 102 31 results result NNS cord-319100-3gdawhfn 102 32 that that WDT cord-319100-3gdawhfn 102 33 were be VBD cord-319100-3gdawhfn 102 34 very very RB cord-319100-3gdawhfn 102 35 similar similar JJ cord-319100-3gdawhfn 102 36 to to IN cord-319100-3gdawhfn 102 37 the the DT cord-319100-3gdawhfn 102 38 original original JJ cord-319100-3gdawhfn 102 39 results result NNS cord-319100-3gdawhfn 102 40 . . . cord-319100-3gdawhfn 103 1 With with IN cord-319100-3gdawhfn 103 2 the the DT cord-319100-3gdawhfn 103 3 exception exception NN cord-319100-3gdawhfn 103 4 of of IN cord-319100-3gdawhfn 103 5 a a DT cord-319100-3gdawhfn 103 6 sample sample NN cord-319100-3gdawhfn 103 7 that that WDT cord-319100-3gdawhfn 103 8 had have VBD cord-319100-3gdawhfn 103 9 given give VBN cord-319100-3gdawhfn 103 10 a a DT cord-319100-3gdawhfn 103 11 result result NN cord-319100-3gdawhfn 103 12 ( ( -LRB- cord-319100-3gdawhfn 103 13 Ct=38.4 ct=38.4 NN cord-319100-3gdawhfn 103 14 ) ) -RRB- cord-319100-3gdawhfn 103 15 close close RB cord-319100-3gdawhfn 103 16 to to IN cord-319100-3gdawhfn 103 17 the the DT cord-319100-3gdawhfn 103 18 limit limit NN cord-319100-3gdawhfn 103 19 of of IN cord-319100-3gdawhfn 103 20 detection detection NN cord-319100-3gdawhfn 103 21 , , , cord-319100-3gdawhfn 103 22 the the DT cord-319100-3gdawhfn 103 23 mean mean JJ cord-319100-3gdawhfn 103 24 variation variation NN cord-319100-3gdawhfn 103 25 for for IN cord-319100-3gdawhfn 103 26 the the DT cord-319100-3gdawhfn 103 27 other other JJ cord-319100-3gdawhfn 103 28 14 14 CD cord-319100-3gdawhfn 103 29 samples sample NNS cord-319100-3gdawhfn 103 30 was be VBD cord-319100-3gdawhfn 103 31 very very RB cord-319100-3gdawhfn 103 32 similar similar JJ cord-319100-3gdawhfn 103 33 ( ( -LRB- cord-319100-3gdawhfn 103 34 0.6 0.6 CD cord-319100-3gdawhfn 103 35 - - SYM cord-319100-3gdawhfn 103 36 1.0 1.0 CD cord-319100-3gdawhfn 103 37 ) ) -RRB- cord-319100-3gdawhfn 104 1 Ct Ct NNP cord-319100-3gdawhfn 104 2 lower low JJR cord-319100-3gdawhfn 104 3 than than IN cord-319100-3gdawhfn 104 4 the the DT cord-319100-3gdawhfn 104 5 original original JJ cord-319100-3gdawhfn 104 6 result result NN cord-319100-3gdawhfn 104 7 . . . cord-319100-3gdawhfn 105 1 When when WRB cord-319100-3gdawhfn 105 2 the the DT cord-319100-3gdawhfn 105 3 influenza influenza NN cord-319100-3gdawhfn 105 4 virus virus NN cord-319100-3gdawhfn 105 5 and and CC cord-319100-3gdawhfn 105 6 its -PRON- PRP$ cord-319100-3gdawhfn 105 7 homologous homologous JJ cord-319100-3gdawhfn 105 8 purified purify VBN cord-319100-3gdawhfn 105 9 RNA RNA NNP cord-319100-3gdawhfn 105 10 were be VBD cord-319100-3gdawhfn 105 11 tested test VBN cord-319100-3gdawhfn 105 12 ( ( -LRB- cord-319100-3gdawhfn 105 13 Table Table NNP cord-319100-3gdawhfn 105 14 4 4 CD cord-319100-3gdawhfn 105 15 ) ) -RRB- cord-319100-3gdawhfn 105 16 , , , cord-319100-3gdawhfn 105 17 similar similar JJ cord-319100-3gdawhfn 105 18 trends trend NNS cord-319100-3gdawhfn 105 19 were be VBD cord-319100-3gdawhfn 105 20 noticed notice VBN cord-319100-3gdawhfn 105 21 for for IN cord-319100-3gdawhfn 105 22 the the DT cord-319100-3gdawhfn 105 23 RNA RNA NNP cord-319100-3gdawhfn 105 24 results result NNS cord-319100-3gdawhfn 105 25 as as IN cord-319100-3gdawhfn 105 26 had have VBD cord-319100-3gdawhfn 105 27 been be VBN cord-319100-3gdawhfn 105 28 observed observe VBN cord-319100-3gdawhfn 105 29 for for IN cord-319100-3gdawhfn 105 30 SARS SARS NNP cord-319100-3gdawhfn 105 31 - - HYPH cord-319100-3gdawhfn 105 32 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 105 33 . . . cord-319100-3gdawhfn 106 1 The the DT cord-319100-3gdawhfn 106 2 results result NNS cord-319100-3gdawhfn 106 3 for for IN cord-319100-3gdawhfn 106 4 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 106 5 and and CC cord-319100-3gdawhfn 106 6 PBS PBS NNP cord-319100-3gdawhfn 106 7 were be VBD cord-319100-3gdawhfn 106 8 comparable comparable JJ cord-319100-3gdawhfn 106 9 but but CC cord-319100-3gdawhfn 106 10 no no DT cord-319100-3gdawhfn 106 11 RNA RNA NNP cord-319100-3gdawhfn 106 12 was be VBD cord-319100-3gdawhfn 106 13 detected detect VBN cord-319100-3gdawhfn 106 14 in in IN cord-319100-3gdawhfn 106 15 the the DT cord-319100-3gdawhfn 106 16 dilutions dilution NNS cord-319100-3gdawhfn 106 17 in in IN cord-319100-3gdawhfn 106 18 VTMs VTMs NNP cord-319100-3gdawhfn 106 19 2 2 CD cord-319100-3gdawhfn 106 20 - - SYM cord-319100-3gdawhfn 106 21 4 4 CD cord-319100-3gdawhfn 106 22 . . . cord-319100-3gdawhfn 107 1 The the DT cord-319100-3gdawhfn 107 2 results result NNS cord-319100-3gdawhfn 107 3 for for IN cord-319100-3gdawhfn 107 4 all all DT cord-319100-3gdawhfn 107 5 solutions solution NNS cord-319100-3gdawhfn 107 6 were be VBD cord-319100-3gdawhfn 107 7 similar similar JJ cord-319100-3gdawhfn 107 8 at at IN cord-319100-3gdawhfn 107 9 each each DT cord-319100-3gdawhfn 107 10 dilution dilution NN cord-319100-3gdawhfn 107 11 when when WRB cord-319100-3gdawhfn 107 12 whole whole JJ cord-319100-3gdawhfn 107 13 virus virus NN cord-319100-3gdawhfn 107 14 was be VBD cord-319100-3gdawhfn 107 15 tested test VBN cord-319100-3gdawhfn 107 16 ( ( -LRB- cord-319100-3gdawhfn 107 17 data datum NNS cord-319100-3gdawhfn 107 18 not not RB cord-319100-3gdawhfn 107 19 included include VBN cord-319100-3gdawhfn 107 20 ) ) -RRB- cord-319100-3gdawhfn 107 21 . . . cord-319100-3gdawhfn 107 22 _SP cord-319100-3gdawhfn 108 1 Finally finally RB cord-319100-3gdawhfn 108 2 , , , cord-319100-3gdawhfn 108 3 to to TO cord-319100-3gdawhfn 108 4 establish establish VB cord-319100-3gdawhfn 108 5 whether whether IN cord-319100-3gdawhfn 108 6 these these DT cord-319100-3gdawhfn 108 7 effects effect NNS cord-319100-3gdawhfn 108 8 were be VBD cord-319100-3gdawhfn 108 9 limited limit VBN cord-319100-3gdawhfn 108 10 to to IN cord-319100-3gdawhfn 108 11 testing testing NN cord-319100-3gdawhfn 108 12 of of IN cord-319100-3gdawhfn 108 13 RNA RNA NNP cord-319100-3gdawhfn 108 14 viruses virus NNS cord-319100-3gdawhfn 108 15 , , , cord-319100-3gdawhfn 108 16 a a DT cord-319100-3gdawhfn 108 17 sample sample NN cord-319100-3gdawhfn 108 18 of of IN cord-319100-3gdawhfn 108 19 bovine bovine JJ cord-319100-3gdawhfn 108 20 herpesvirus-1 herpesvirus-1 NNP cord-319100-3gdawhfn 108 21 was be VBD cord-319100-3gdawhfn 108 22 tested test VBN cord-319100-3gdawhfn 108 23 in in IN cord-319100-3gdawhfn 108 24 the the DT cord-319100-3gdawhfn 108 25 same same JJ cord-319100-3gdawhfn 108 26 model model NN cord-319100-3gdawhfn 108 27 and and CC cord-319100-3gdawhfn 108 28 range range NN cord-319100-3gdawhfn 108 29 of of IN cord-319100-3gdawhfn 108 30 dilutions dilution NNS cord-319100-3gdawhfn 108 31 . . . cord-319100-3gdawhfn 109 1 The the DT cord-319100-3gdawhfn 109 2 results result NNS cord-319100-3gdawhfn 109 3 for for IN cord-319100-3gdawhfn 109 4 each each DT cord-319100-3gdawhfn 109 5 of of IN cord-319100-3gdawhfn 109 6 the the DT cord-319100-3gdawhfn 109 7 VTMs vtm NNS cord-319100-3gdawhfn 109 8 and and CC cord-319100-3gdawhfn 109 9 PBS PBS NNP cord-319100-3gdawhfn 109 10 containing contain VBG cord-319100-3gdawhfn 109 11 the the DT cord-319100-3gdawhfn 109 12 highest high JJS cord-319100-3gdawhfn 109 13 concentrations concentration NNS cord-319100-3gdawhfn 109 14 of of IN cord-319100-3gdawhfn 109 15 herpesvirus herpesvirus NN cord-319100-3gdawhfn 109 16 DNA DNA NNP cord-319100-3gdawhfn 109 17 were be VBD cord-319100-3gdawhfn 109 18 similar similar JJ cord-319100-3gdawhfn 109 19 ( ( -LRB- cord-319100-3gdawhfn 109 20 Table table NN cord-319100-3gdawhfn 109 21 5 5 CD cord-319100-3gdawhfn 109 22 ) ) -RRB- cord-319100-3gdawhfn 109 23 although although IN cord-319100-3gdawhfn 109 24 there there EX cord-319100-3gdawhfn 109 25 was be VBD cord-319100-3gdawhfn 109 26 a a DT cord-319100-3gdawhfn 109 27 distinct distinct JJ cord-319100-3gdawhfn 109 28 trend trend NN cord-319100-3gdawhfn 109 29 towards towards IN cord-319100-3gdawhfn 109 30 higher high JJR cord-319100-3gdawhfn 109 31 Ct ct JJ cord-319100-3gdawhfn 109 32 values value NNS cord-319100-3gdawhfn 109 33 for for IN cord-319100-3gdawhfn 109 34 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 109 35 and and CC cord-319100-3gdawhfn 109 36 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 109 37 . . . cord-319100-3gdawhfn 110 1 The the DT cord-319100-3gdawhfn 110 2 preparations preparation NNS cord-319100-3gdawhfn 110 3 of of IN cord-319100-3gdawhfn 110 4 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 110 5 and and CC cord-319100-3gdawhfn 110 6 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 110 7 with with IN cord-319100-3gdawhfn 110 8 the the DT cord-319100-3gdawhfn 110 9 lowest low JJS cord-319100-3gdawhfn 110 10 concentrations concentration NNS cord-319100-3gdawhfn 110 11 of of IN cord-319100-3gdawhfn 110 12 DNA DNA NNP cord-319100-3gdawhfn 110 13 gave give VBD cord-319100-3gdawhfn 110 14 negative negative JJ cord-319100-3gdawhfn 110 15 results result NNS cord-319100-3gdawhfn 110 16 . . . cord-319100-3gdawhfn 111 1 To to TO cord-319100-3gdawhfn 111 2 confirm confirm VB cord-319100-3gdawhfn 111 3 that that IN cord-319100-3gdawhfn 111 4 the the DT cord-319100-3gdawhfn 111 5 differences difference NNS cord-319100-3gdawhfn 111 6 observed observe VBN cord-319100-3gdawhfn 111 7 were be VBD cord-319100-3gdawhfn 111 8 not not RB cord-319100-3gdawhfn 111 9 due due JJ cord-319100-3gdawhfn 111 10 to to IN cord-319100-3gdawhfn 111 11 random random JJ cord-319100-3gdawhfn 111 12 variation variation NN cord-319100-3gdawhfn 111 13 around around IN cord-319100-3gdawhfn 111 14 the the DT cord-319100-3gdawhfn 111 15 limit limit NN cord-319100-3gdawhfn 111 16 of of IN cord-319100-3gdawhfn 111 17 detection detection NN cord-319100-3gdawhfn 111 18 of of IN cord-319100-3gdawhfn 111 19 the the DT cord-319100-3gdawhfn 111 20 assay assay NN cord-319100-3gdawhfn 111 21 , , , cord-319100-3gdawhfn 111 22 eight eight CD cord-319100-3gdawhfn 111 23 replicates replicate NNS cord-319100-3gdawhfn 111 24 for for IN cord-319100-3gdawhfn 111 25 the the DT cord-319100-3gdawhfn 111 26 two two CD cord-319100-3gdawhfn 111 27 lowest low JJS cord-319100-3gdawhfn 111 28 DNA dna NN cord-319100-3gdawhfn 111 29 concentrations concentration NNS cord-319100-3gdawhfn 111 30 ( ( -LRB- cord-319100-3gdawhfn 111 31 highest high JJS cord-319100-3gdawhfn 111 32 two two CD cord-319100-3gdawhfn 111 33 dilutions dilution NNS cord-319100-3gdawhfn 111 34 of of IN cord-319100-3gdawhfn 111 35 DNA dna NN cord-319100-3gdawhfn 111 36 ) ) -RRB- cord-319100-3gdawhfn 111 37 were be VBD cord-319100-3gdawhfn 111 38 then then RB cord-319100-3gdawhfn 111 39 tested test VBN cord-319100-3gdawhfn 111 40 ( ( -LRB- cord-319100-3gdawhfn 111 41 Table Table NNP cord-319100-3gdawhfn 111 42 6 6 CD cord-319100-3gdawhfn 111 43 ) ) -RRB- cord-319100-3gdawhfn 111 44 . . . cord-319100-3gdawhfn 112 1 These these DT cord-319100-3gdawhfn 112 2 results result NNS cord-319100-3gdawhfn 112 3 confirmed confirm VBD cord-319100-3gdawhfn 112 4 that that IN cord-319100-3gdawhfn 112 5 there there EX cord-319100-3gdawhfn 112 6 had have VBD cord-319100-3gdawhfn 112 7 been be VBN cord-319100-3gdawhfn 112 8 some some DT cord-319100-3gdawhfn 112 9 impact impact NN cord-319100-3gdawhfn 112 10 of of IN cord-319100-3gdawhfn 112 11 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 112 12 , , , cord-319100-3gdawhfn 112 13 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 112 14 and and CC cord-319100-3gdawhfn 112 15 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 112 16 on on IN cord-319100-3gdawhfn 112 17 the the DT cord-319100-3gdawhfn 112 18 stability stability NN cord-319100-3gdawhfn 112 19 of of IN cord-319100-3gdawhfn 112 20 the the DT cord-319100-3gdawhfn 112 21 purified purify VBN cord-319100-3gdawhfn 112 22 DNA dna NN cord-319100-3gdawhfn 112 23 extracts extract NNS cord-319100-3gdawhfn 112 24 . . . cord-319100-3gdawhfn 113 1 As as IN cord-319100-3gdawhfn 113 2 had have VBD cord-319100-3gdawhfn 113 3 been be VBN cord-319100-3gdawhfn 113 4 noted note VBN cord-319100-3gdawhfn 113 5 for for IN cord-319100-3gdawhfn 113 6 previous previous JJ cord-319100-3gdawhfn 113 7 experiments experiment NNS cord-319100-3gdawhfn 113 8 , , , cord-319100-3gdawhfn 113 9 the the DT cord-319100-3gdawhfn 113 10 XIPC XIPC NNP cord-319100-3gdawhfn 113 11 gave give VBD cord-319100-3gdawhfn 113 12 highly highly RB cord-319100-3gdawhfn 113 13 reproducible reproducible JJ cord-319100-3gdawhfn 113 14 results result NNS cord-319100-3gdawhfn 113 15 for for IN cord-319100-3gdawhfn 113 16 all all DT cord-319100-3gdawhfn 113 17 samples sample NNS cord-319100-3gdawhfn 113 18 tested test VBN cord-319100-3gdawhfn 113 19 . . . cord-319100-3gdawhfn 113 20 _SP cord-319100-3gdawhfn 114 1 When when WRB cord-319100-3gdawhfn 114 2 the the DT cord-319100-3gdawhfn 114 3 dilutions dilution NNS cord-319100-3gdawhfn 114 4 of of IN cord-319100-3gdawhfn 114 5 bovine bovine JJ cord-319100-3gdawhfn 114 6 herpesvirus herpesvirus NN cord-319100-3gdawhfn 114 7 were be VBD cord-319100-3gdawhfn 114 8 tested test VBN cord-319100-3gdawhfn 114 9 , , , cord-319100-3gdawhfn 114 10 the the DT cord-319100-3gdawhfn 114 11 adverse adverse JJ cord-319100-3gdawhfn 114 12 effects effect NNS cord-319100-3gdawhfn 114 13 that that WDT cord-319100-3gdawhfn 114 14 had have VBD cord-319100-3gdawhfn 114 15 been be VBN cord-319100-3gdawhfn 114 16 observed observe VBN cord-319100-3gdawhfn 114 17 with with IN cord-319100-3gdawhfn 114 18 the the DT cord-319100-3gdawhfn 114 19 purified purify VBN cord-319100-3gdawhfn 114 20 DNA DNA NNP cord-319100-3gdawhfn 114 21 were be VBD cord-319100-3gdawhfn 114 22 again again RB cord-319100-3gdawhfn 114 23 noted note VBN cord-319100-3gdawhfn 114 24 but but CC cord-319100-3gdawhfn 114 25 perhaps perhaps RB cord-319100-3gdawhfn 114 26 in in IN cord-319100-3gdawhfn 114 27 a a DT cord-319100-3gdawhfn 114 28 more more RBR cord-319100-3gdawhfn 114 29 profound profound JJ cord-319100-3gdawhfn 114 30 manner manner NN cord-319100-3gdawhfn 114 31 Table table NN cord-319100-3gdawhfn 114 32 7 7 CD cord-319100-3gdawhfn 114 33 ) ) -RRB- cord-319100-3gdawhfn 114 34 . . . cord-319100-3gdawhfn 115 1 Reduced reduced JJ cord-319100-3gdawhfn 115 2 sensitivity sensitivity NN cord-319100-3gdawhfn 115 3 was be VBD cord-319100-3gdawhfn 115 4 observed observe VBN cord-319100-3gdawhfn 115 5 with with IN cord-319100-3gdawhfn 115 6 samples sample NNS cord-319100-3gdawhfn 115 7 diluted dilute VBN cord-319100-3gdawhfn 115 8 in in IN cord-319100-3gdawhfn 115 9 VTM-2 VTM-2 NNP cord-319100-3gdawhfn 115 10 , , , cord-319100-3gdawhfn 115 11 VTM-3 VTM-3 NNP cord-319100-3gdawhfn 115 12 and and CC cord-319100-3gdawhfn 115 13 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 115 14 . . . cord-319100-3gdawhfn 116 1 In in IN cord-319100-3gdawhfn 116 2 order order NN cord-319100-3gdawhfn 116 3 to to TO cord-319100-3gdawhfn 116 4 further further RB cord-319100-3gdawhfn 116 5 confirm confirm VB cord-319100-3gdawhfn 116 6 that that IN cord-319100-3gdawhfn 116 7 the the DT cord-319100-3gdawhfn 116 8 differences difference NNS cord-319100-3gdawhfn 116 9 observed observe VBN cord-319100-3gdawhfn 116 10 between between IN cord-319100-3gdawhfn 116 11 the the DT cord-319100-3gdawhfn 116 12 different different JJ cord-319100-3gdawhfn 116 13 dilutions dilution NNS cord-319100-3gdawhfn 116 14 of of IN cord-319100-3gdawhfn 116 15 virus virus NN cord-319100-3gdawhfn 116 16 were be VBD cord-319100-3gdawhfn 116 17 not not RB cord-319100-3gdawhfn 116 18 due due JJ cord-319100-3gdawhfn 116 19 to to IN cord-319100-3gdawhfn 116 20 random random JJ cord-319100-3gdawhfn 116 21 variation variation NN cord-319100-3gdawhfn 116 22 around around IN cord-319100-3gdawhfn 116 23 the the DT cord-319100-3gdawhfn 116 24 limit limit NN cord-319100-3gdawhfn 116 25 of of IN cord-319100-3gdawhfn 116 26 detection detection NN cord-319100-3gdawhfn 116 27 of of IN cord-319100-3gdawhfn 116 28 the the DT cord-319100-3gdawhfn 116 29 assay assay NN cord-319100-3gdawhfn 116 30 , , , cord-319100-3gdawhfn 116 31 seven seven CD cord-319100-3gdawhfn 116 32 replicates replicate NNS cord-319100-3gdawhfn 116 33 of of IN cord-319100-3gdawhfn 116 34 each each DT cord-319100-3gdawhfn 116 35 sample sample NN cord-319100-3gdawhfn 116 36 from from IN cord-319100-3gdawhfn 116 37 the the DT cord-319100-3gdawhfn 116 38 last last JJ cord-319100-3gdawhfn 116 39 2 2 CD cord-319100-3gdawhfn 116 40 dilutions dilution NNS cord-319100-3gdawhfn 116 41 that that WDT cord-319100-3gdawhfn 116 42 were be VBD cord-319100-3gdawhfn 116 43 positive positive JJ cord-319100-3gdawhfn 116 44 in in IN cord-319100-3gdawhfn 116 45 PBS PBS NNP cord-319100-3gdawhfn 116 46 were be VBD cord-319100-3gdawhfn 116 47 tested test VBN cord-319100-3gdawhfn 116 48 again again RB cord-319100-3gdawhfn 116 49 . . . cord-319100-3gdawhfn 117 1 The the DT cord-319100-3gdawhfn 117 2 results result NNS cord-319100-3gdawhfn 117 3 ( ( -LRB- cord-319100-3gdawhfn 117 4 _SP cord-319100-3gdawhfn 117 5 The the DT cord-319100-3gdawhfn 117 6 experiments experiment NNS cord-319100-3gdawhfn 117 7 undertaken undertake VBN cord-319100-3gdawhfn 117 8 have have VBP cord-319100-3gdawhfn 117 9 shown show VBN cord-319100-3gdawhfn 117 10 that that IN cord-319100-3gdawhfn 117 11 some some DT cord-319100-3gdawhfn 117 12 of of IN cord-319100-3gdawhfn 117 13 the the DT cord-319100-3gdawhfn 117 14 VTM VTM NNP cord-319100-3gdawhfn 117 15 solutions solution NNS cord-319100-3gdawhfn 117 16 examined examine VBN cord-319100-3gdawhfn 117 17 had have VBD cord-319100-3gdawhfn 117 18 a a DT cord-319100-3gdawhfn 117 19 significant significant JJ cord-319100-3gdawhfn 117 20 and and CC cord-319100-3gdawhfn 117 21 deleterious deleterious JJ cord-319100-3gdawhfn 117 22 impact impact NN cord-319100-3gdawhfn 117 23 on on IN cord-319100-3gdawhfn 117 24 purified purify VBN cord-319100-3gdawhfn 117 25 viral viral JJ cord-319100-3gdawhfn 117 26 RNA RNA NNP cord-319100-3gdawhfn 117 27 and and CC cord-319100-3gdawhfn 117 28 variable variable JJ cord-319100-3gdawhfn 117 29 effects effect NNS cord-319100-3gdawhfn 117 30 on on IN cord-319100-3gdawhfn 117 31 DNA dna NN cord-319100-3gdawhfn 117 32 . . . cord-319100-3gdawhfn 118 1 The the DT cord-319100-3gdawhfn 118 2 synthetic synthetic JJ cord-319100-3gdawhfn 118 3 XIPC XIPC NNP cord-319100-3gdawhfn 118 4 RNA RNA NNP cord-319100-3gdawhfn 118 5 that that WDT cord-319100-3gdawhfn 118 6 was be VBD cord-319100-3gdawhfn 118 7 used use VBN cord-319100-3gdawhfn 118 8 throughout throughout IN cord-319100-3gdawhfn 118 9 this this DT cord-319100-3gdawhfn 118 10 study study NN cord-319100-3gdawhfn 118 11 was be VBD cord-319100-3gdawhfn 118 12 not not RB cord-319100-3gdawhfn 118 13 affected affect VBN cord-319100-3gdawhfn 118 14 because because IN cord-319100-3gdawhfn 118 15 it -PRON- PRP cord-319100-3gdawhfn 118 16 was be VBD cord-319100-3gdawhfn 118 17 prepared prepare VBN cord-319100-3gdawhfn 118 18 in in IN cord-319100-3gdawhfn 118 19 a a DT cord-319100-3gdawhfn 118 20 tRNA trna NN cord-319100-3gdawhfn 118 21 solution solution NN cord-319100-3gdawhfn 118 22 and and CC cord-319100-3gdawhfn 118 23 included include VBN cord-319100-3gdawhfn 118 24 in in IN cord-319100-3gdawhfn 118 25 the the DT cord-319100-3gdawhfn 118 26 sample sample NN cord-319100-3gdawhfn 118 27 lysis lysis NN cord-319100-3gdawhfn 118 28 buffer buffer NN cord-319100-3gdawhfn 118 29 which which WDT cord-319100-3gdawhfn 118 30 includes include VBZ cord-319100-3gdawhfn 118 31 inhibitors inhibitor NNS cord-319100-3gdawhfn 118 32 of of IN cord-319100-3gdawhfn 118 33 nuclease nuclease NNP cord-319100-3gdawhfn 118 34 activity activity NN cord-319100-3gdawhfn 118 35 and and CC cord-319100-3gdawhfn 118 36 the the DT cord-319100-3gdawhfn 118 37 sample sample NN cord-319100-3gdawhfn 118 38 is be VBZ cord-319100-3gdawhfn 118 39 extracted extract VBN cord-319100-3gdawhfn 118 40 immediately immediately RB cord-319100-3gdawhfn 118 41 after after IN cord-319100-3gdawhfn 118 42 addition addition NN cord-319100-3gdawhfn 118 43 of of IN cord-319100-3gdawhfn 118 44 the the DT cord-319100-3gdawhfn 118 45 buffer buffer NN cord-319100-3gdawhfn 118 46 . . . cord-319100-3gdawhfn 119 1 However however RB cord-319100-3gdawhfn 119 2 , , , cord-319100-3gdawhfn 119 3 to to TO cord-319100-3gdawhfn 119 4 confirm confirm VB cord-319100-3gdawhfn 119 5 that that IN cord-319100-3gdawhfn 119 6 the the DT cord-319100-3gdawhfn 119 7 XIPC XIPC NNP cord-319100-3gdawhfn 119 8 construct construct NN cord-319100-3gdawhfn 119 9 could could MD cord-319100-3gdawhfn 119 10 be be VB cord-319100-3gdawhfn 119 11 affected affect VBN cord-319100-3gdawhfn 119 12 by by IN cord-319100-3gdawhfn 119 13 components component NNS cord-319100-3gdawhfn 119 14 in in IN cord-319100-3gdawhfn 119 15 the the DT cord-319100-3gdawhfn 119 16 VTMs vtm NNS cord-319100-3gdawhfn 119 17 under under IN cord-319100-3gdawhfn 119 18 study study NN cord-319100-3gdawhfn 119 19 , , , cord-319100-3gdawhfn 119 20 two two CD cord-319100-3gdawhfn 119 21 concentrations concentration NNS cord-319100-3gdawhfn 119 22 of of IN cord-319100-3gdawhfn 119 23 XIPC XIPC NNP cord-319100-3gdawhfn 119 24 were be VBD cord-319100-3gdawhfn 119 25 prepared prepare VBN cord-319100-3gdawhfn 119 26 in in IN cord-319100-3gdawhfn 119 27 PBS PBS NNP cord-319100-3gdawhfn 119 28 and and CC cord-319100-3gdawhfn 119 29 each each DT cord-319100-3gdawhfn 119 30 of of IN cord-319100-3gdawhfn 119 31 the the DT cord-319100-3gdawhfn 119 32 VTM VTM NNP cord-319100-3gdawhfn 119 33 solutions solution NNS cord-319100-3gdawhfn 119 34 and and CC cord-319100-3gdawhfn 119 35 tested test VBN cord-319100-3gdawhfn 119 36 after after IN cord-319100-3gdawhfn 119 37 being be VBG cord-319100-3gdawhfn 119 38 held hold VBN cord-319100-3gdawhfn 119 39 at at IN cord-319100-3gdawhfn 119 40 room room NN cord-319100-3gdawhfn 119 41 temperature temperature NN cord-319100-3gdawhfn 119 42 for for IN cord-319100-3gdawhfn 119 43 1 1 CD cord-319100-3gdawhfn 119 44 or or CC cord-319100-3gdawhfn 119 45 48 48 CD cord-319100-3gdawhfn 119 46 hours hour NNS cord-319100-3gdawhfn 119 47 . . . cord-319100-3gdawhfn 120 1 The the DT cord-319100-3gdawhfn 120 2 adverse adverse JJ cord-319100-3gdawhfn 120 3 effect effect NN cord-319100-3gdawhfn 120 4 of of IN cord-319100-3gdawhfn 120 5 each each DT cord-319100-3gdawhfn 120 6 commercial commercial JJ cord-319100-3gdawhfn 120 7 VTM VTM NNP cord-319100-3gdawhfn 120 8 ( ( -LRB- cord-319100-3gdawhfn 120 9 Table Table NNP cord-319100-3gdawhfn 120 10 9 9 CD cord-319100-3gdawhfn 120 11 ) ) -RRB- cord-319100-3gdawhfn 120 12 was be VBD cord-319100-3gdawhfn 120 13 apparent apparent JJ cord-319100-3gdawhfn 120 14 within within IN cord-319100-3gdawhfn 120 15 the the DT cord-319100-3gdawhfn 120 16 first first JJ cord-319100-3gdawhfn 120 17 hour hour NN cord-319100-3gdawhfn 120 18 and and CC cord-319100-3gdawhfn 120 19 no no DT cord-319100-3gdawhfn 120 20 RNA RNA NNP cord-319100-3gdawhfn 120 21 was be VBD cord-319100-3gdawhfn 120 22 detected detect VBN cord-319100-3gdawhfn 120 23 after after IN cord-319100-3gdawhfn 120 24 48 48 CD cord-319100-3gdawhfn 120 25 hours hour NNS cord-319100-3gdawhfn 120 26 . . . cord-319100-3gdawhfn 121 1 Table table NN cord-319100-3gdawhfn 121 2 9 9 CD cord-319100-3gdawhfn 122 1 -qPCR -qPCR : cord-319100-3gdawhfn 122 2 - - HYPH cord-319100-3gdawhfn 122 3 PCR PCR NNP cord-319100-3gdawhfn 122 4 Ct Ct NNP cord-319100-3gdawhfn 122 5 values value NNS cord-319100-3gdawhfn 122 6 for for IN cord-319100-3gdawhfn 122 7 the the DT cord-319100-3gdawhfn 122 8 exogenous exogenous JJ cord-319100-3gdawhfn 122 9 synthetic synthetic JJ cord-319100-3gdawhfn 122 10 RNA RNA NNP cord-319100-3gdawhfn 122 11 when when WRB cord-319100-3gdawhfn 122 12 diluted dilute VBN cord-319100-3gdawhfn 122 13 in in IN cord-319100-3gdawhfn 122 14 different different JJ cord-319100-3gdawhfn 122 15 viral viral JJ cord-319100-3gdawhfn 122 16 transport transport NN cord-319100-3gdawhfn 122 17 media medium NNS cord-319100-3gdawhfn 122 18 and and CC cord-319100-3gdawhfn 122 19 tested test VBD cord-319100-3gdawhfn 122 20 immediately immediately RB cord-319100-3gdawhfn 122 21 and and CC cord-319100-3gdawhfn 122 22 after after IN cord-319100-3gdawhfn 122 23 holding hold VBG cord-319100-3gdawhfn 122 24 at at IN cord-319100-3gdawhfn 122 25 room room NN cord-319100-3gdawhfn 122 26 temperature temperature NN cord-319100-3gdawhfn 122 27 for for IN cord-319100-3gdawhfn 122 28 2 2 CD cord-319100-3gdawhfn 122 29 days day NNS cord-319100-3gdawhfn 123 1 The the DT cord-319100-3gdawhfn 123 2 results result NNS cord-319100-3gdawhfn 123 3 of of IN cord-319100-3gdawhfn 123 4 these these DT cord-319100-3gdawhfn 123 5 studies study NNS cord-319100-3gdawhfn 123 6 clearly clearly RB cord-319100-3gdawhfn 123 7 indicate indicate VBP cord-319100-3gdawhfn 123 8 that that IN cord-319100-3gdawhfn 123 9 the the DT cord-319100-3gdawhfn 123 10 commercially commercially RB cord-319100-3gdawhfn 123 11 prepared prepare VBN cord-319100-3gdawhfn 123 12 VTM VTM NNP cord-319100-3gdawhfn 123 13 solutions solution NNS cord-319100-3gdawhfn 123 14 have have VBP cord-319100-3gdawhfn 123 15 had have VBN cord-319100-3gdawhfn 123 16 an an DT cord-319100-3gdawhfn 123 17 adverse adverse JJ cord-319100-3gdawhfn 123 18 impact impact NN cord-319100-3gdawhfn 123 19 on on IN cord-319100-3gdawhfn 123 20 the the DT cord-319100-3gdawhfn 123 21 ability ability NN cord-319100-3gdawhfn 123 22 to to TO cord-319100-3gdawhfn 123 23 detect detect VB cord-319100-3gdawhfn 123 24 both both DT cord-319100-3gdawhfn 123 25 SARS SARS NNP cord-319100-3gdawhfn 123 26 - - HYPH cord-319100-3gdawhfn 123 27 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 123 28 and and CC cord-319100-3gdawhfn 123 29 influenza influenza NN cord-319100-3gdawhfn 123 30 RNA RNA NNP cord-319100-3gdawhfn 123 31 . . . cord-319100-3gdawhfn 124 1 The the DT cord-319100-3gdawhfn 124 2 results result NNS cord-319100-3gdawhfn 124 3 for for IN cord-319100-3gdawhfn 124 4 the the DT cord-319100-3gdawhfn 124 5 RNA RNA NNP cord-319100-3gdawhfn 124 6 preparations preparation NNS cord-319100-3gdawhfn 124 7 diluted dilute VBN cord-319100-3gdawhfn 124 8 in in IN cord-319100-3gdawhfn 124 9 the the DT cord-319100-3gdawhfn 124 10 commercial commercial JJ cord-319100-3gdawhfn 124 11 VTMs vtm NNS cord-319100-3gdawhfn 124 12 would would MD cord-319100-3gdawhfn 124 13 suggest suggest VB cord-319100-3gdawhfn 124 14 that that IN cord-319100-3gdawhfn 124 15 there there EX cord-319100-3gdawhfn 124 16 are be VBP cord-319100-3gdawhfn 124 17 components component NNS cord-319100-3gdawhfn 124 18 of of IN cord-319100-3gdawhfn 124 19 these these DT cord-319100-3gdawhfn 124 20 VTMs vtm NNS cord-319100-3gdawhfn 124 21 that that WDT cord-319100-3gdawhfn 124 22 have have VBP cord-319100-3gdawhfn 124 23 prevented prevent VBN cord-319100-3gdawhfn 124 24 the the DT cord-319100-3gdawhfn 124 25 detection detection NN cord-319100-3gdawhfn 124 26 of of IN cord-319100-3gdawhfn 124 27 the the DT cord-319100-3gdawhfn 124 28 RNA RNA NNP cord-319100-3gdawhfn 124 29 in in IN cord-319100-3gdawhfn 124 30 these these DT cord-319100-3gdawhfn 124 31 samples sample NNS cord-319100-3gdawhfn 124 32 . . . cord-319100-3gdawhfn 125 1 As as IN cord-319100-3gdawhfn 125 2 the the DT cord-319100-3gdawhfn 125 3 detection detection NN cord-319100-3gdawhfn 125 4 of of IN cord-319100-3gdawhfn 125 5 the the DT cord-319100-3gdawhfn 125 6 XIPC XIPC NNP cord-319100-3gdawhfn 125 7 was be VBD cord-319100-3gdawhfn 125 8 not not RB cord-319100-3gdawhfn 125 9 affected affect VBN cord-319100-3gdawhfn 125 10 , , , cord-319100-3gdawhfn 125 11 we -PRON- PRP cord-319100-3gdawhfn 125 12 propose propose VBP cord-319100-3gdawhfn 125 13 that that IN cord-319100-3gdawhfn 125 14 these these DT cord-319100-3gdawhfn 125 15 results result NNS cord-319100-3gdawhfn 125 16 provide provide VBP cord-319100-3gdawhfn 125 17 unequivocal unequivocal JJ cord-319100-3gdawhfn 125 18 evidence evidence NN cord-319100-3gdawhfn 125 19 of of IN cord-319100-3gdawhfn 125 20 the the DT cord-319100-3gdawhfn 125 21 presence presence NN cord-319100-3gdawhfn 125 22 of of IN cord-319100-3gdawhfn 125 23 a a DT cord-319100-3gdawhfn 125 24 nuclease(s nuclease(s NNP cord-319100-3gdawhfn 125 25 ) ) -RRB- cord-319100-3gdawhfn 125 26 or or CC cord-319100-3gdawhfn 125 27 similar similar JJ cord-319100-3gdawhfn 125 28 substance substance NN cord-319100-3gdawhfn 125 29 in in IN cord-319100-3gdawhfn 125 30 these these DT cord-319100-3gdawhfn 125 31 VTMs vtm NNS cord-319100-3gdawhfn 125 32 . . . cord-319100-3gdawhfn 126 1 The the DT cord-319100-3gdawhfn 126 2 impact impact NN cord-319100-3gdawhfn 126 3 on on IN cord-319100-3gdawhfn 126 4 these these DT cord-319100-3gdawhfn 126 5 samples sample NNS cord-319100-3gdawhfn 126 6 was be VBD cord-319100-3gdawhfn 126 7 rapid rapid JJ cord-319100-3gdawhfn 126 8 as as IN cord-319100-3gdawhfn 126 9 all all DT cord-319100-3gdawhfn 126 10 samples sample NNS cord-319100-3gdawhfn 126 11 were be VBD cord-319100-3gdawhfn 126 12 extracted extract VBN cord-319100-3gdawhfn 126 13 within within IN cord-319100-3gdawhfn 126 14 one one CD cord-319100-3gdawhfn 126 15 hour hour NN cord-319100-3gdawhfn 126 16 of of IN cord-319100-3gdawhfn 126 17 preparation preparation NN cord-319100-3gdawhfn 126 18 yet yet CC cord-319100-3gdawhfn 126 19 no no DT cord-319100-3gdawhfn 126 20 RNA RNA NNP cord-319100-3gdawhfn 126 21 was be VBD cord-319100-3gdawhfn 126 22 detected detect VBN cord-319100-3gdawhfn 126 23 in in IN cord-319100-3gdawhfn 126 24 a a DT cord-319100-3gdawhfn 126 25 sample sample NN cord-319100-3gdawhfn 126 26 that that WDT cord-319100-3gdawhfn 126 27 was be VBD cord-319100-3gdawhfn 126 28 estimated estimate VBN cord-319100-3gdawhfn 126 29 to to TO cord-319100-3gdawhfn 126 30 contain contain VB cord-319100-3gdawhfn 126 31 more more JJR cord-319100-3gdawhfn 126 32 than than IN cord-319100-3gdawhfn 126 33 3000 3000 CD cord-319100-3gdawhfn 126 34 copies copy NNS cord-319100-3gdawhfn 126 35 of of IN cord-319100-3gdawhfn 126 36 viral viral JJ cord-319100-3gdawhfn 126 37 RNA RNA NNP cord-319100-3gdawhfn 126 38 in in IN cord-319100-3gdawhfn 126 39 a a DT cord-319100-3gdawhfn 126 40 5uL 5ul CD cord-319100-3gdawhfn 126 41 sample sample NN cord-319100-3gdawhfn 126 42 . . . cord-319100-3gdawhfn 127 1 The the DT cord-319100-3gdawhfn 127 2 same same JJ cord-319100-3gdawhfn 127 3 result result NN cord-319100-3gdawhfn 127 4 was be VBD cord-319100-3gdawhfn 127 5 obtained obtain VBN cord-319100-3gdawhfn 127 6 with with IN cord-319100-3gdawhfn 127 7 assays assay NNS cord-319100-3gdawhfn 127 8 that that WDT cord-319100-3gdawhfn 127 9 were be VBD cord-319100-3gdawhfn 127 10 directed direct VBN cord-319100-3gdawhfn 127 11 at at IN cord-319100-3gdawhfn 127 12 3 3 CD cord-319100-3gdawhfn 127 13 different different JJ cord-319100-3gdawhfn 127 14 regions region NNS cord-319100-3gdawhfn 127 15 of of IN cord-319100-3gdawhfn 127 16 the the DT cord-319100-3gdawhfn 127 17 SARS SARS NNP cord-319100-3gdawhfn 127 18 - - HYPH cord-319100-3gdawhfn 127 19 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 127 20 genome genome NN cord-319100-3gdawhfn 127 21 . . . cord-319100-3gdawhfn 128 1 The the DT cord-319100-3gdawhfn 128 2 results result NNS cord-319100-3gdawhfn 128 3 obtained obtain VBN cord-319100-3gdawhfn 128 4 for for IN cord-319100-3gdawhfn 128 5 the the DT cord-319100-3gdawhfn 128 6 virus virus NN cord-319100-3gdawhfn 128 7 samples sample NNS cord-319100-3gdawhfn 128 8 that that WDT cord-319100-3gdawhfn 128 9 were be VBD cord-319100-3gdawhfn 128 10 diluted dilute VBN cord-319100-3gdawhfn 128 11 to to IN cord-319100-3gdawhfn 128 12 similar similar JJ cord-319100-3gdawhfn 128 13 concentrations concentration NNS cord-319100-3gdawhfn 128 14 as as IN cord-319100-3gdawhfn 128 15 the the DT cord-319100-3gdawhfn 128 16 RNA RNA NNP cord-319100-3gdawhfn 128 17 samples sample NNS cord-319100-3gdawhfn 128 18 and and CC cord-319100-3gdawhfn 128 19 held hold VBN cord-319100-3gdawhfn 128 20 for for IN cord-319100-3gdawhfn 128 21 the the DT cord-319100-3gdawhfn 128 22 same same JJ cord-319100-3gdawhfn 128 23 time time NN cord-319100-3gdawhfn 128 24 period period NN cord-319100-3gdawhfn 128 25 also also RB cord-319100-3gdawhfn 128 26 support support VBP cord-319100-3gdawhfn 128 27 this this DT cord-319100-3gdawhfn 128 28 hypothesis hypothesis NN cord-319100-3gdawhfn 128 29 because because IN cord-319100-3gdawhfn 128 30 it -PRON- PRP cord-319100-3gdawhfn 128 31 is be VBZ cord-319100-3gdawhfn 128 32 expected expect VBN cord-319100-3gdawhfn 128 33 that that IN cord-319100-3gdawhfn 128 34 these these DT cord-319100-3gdawhfn 128 35 samples sample NNS cord-319100-3gdawhfn 128 36 contained contain VBD cord-319100-3gdawhfn 128 37 a a DT cord-319100-3gdawhfn 128 38 high high JJ cord-319100-3gdawhfn 128 39 proportion proportion NN cord-319100-3gdawhfn 128 40 of of IN cord-319100-3gdawhfn 128 41 intact intact JJ cord-319100-3gdawhfn 128 42 nucleocapsids nucleocapsid NNS cord-319100-3gdawhfn 128 43 that that WDT cord-319100-3gdawhfn 128 44 offered offer VBD cord-319100-3gdawhfn 128 45 protection protection NN cord-319100-3gdawhfn 128 46 to to IN cord-319100-3gdawhfn 128 47 the the DT cord-319100-3gdawhfn 128 48 viral viral JJ cord-319100-3gdawhfn 128 49 RNA RNA NNP cord-319100-3gdawhfn 128 50 . . . cord-319100-3gdawhfn 129 1 The the DT cord-319100-3gdawhfn 129 2 same same JJ cord-319100-3gdawhfn 129 3 trends trend NNS cord-319100-3gdawhfn 129 4 were be VBD cord-319100-3gdawhfn 129 5 observed observe VBN cord-319100-3gdawhfn 129 6 with with IN cord-319100-3gdawhfn 129 7 the the DT cord-319100-3gdawhfn 129 8 Influenza Influenza NNP cord-319100-3gdawhfn 129 9 A A NNP cord-319100-3gdawhfn 129 10 virus virus NN cord-319100-3gdawhfn 129 11 RNA rna NN cord-319100-3gdawhfn 129 12 and and CC cord-319100-3gdawhfn 129 13 presumptively presumptively RB cord-319100-3gdawhfn 129 14 intact intact JJ cord-319100-3gdawhfn 129 15 virions virion NNS cord-319100-3gdawhfn 129 16 . . . cord-319100-3gdawhfn 130 1 Although although IN cord-319100-3gdawhfn 130 2 the the DT cord-319100-3gdawhfn 130 3 effects effect NNS cord-319100-3gdawhfn 130 4 on on IN cord-319100-3gdawhfn 130 5 the the DT cord-319100-3gdawhfn 130 6 herpesvirus herpesvirus NN cord-319100-3gdawhfn 130 7 samples sample NNS cord-319100-3gdawhfn 130 8 were be VBD cord-319100-3gdawhfn 130 9 less less RBR cord-319100-3gdawhfn 130 10 pronounced pronounce VBN cord-319100-3gdawhfn 130 11 , , , cord-319100-3gdawhfn 130 12 nevertheless nevertheless RB cord-319100-3gdawhfn 130 13 there there EX cord-319100-3gdawhfn 130 14 was be VBD cord-319100-3gdawhfn 130 15 some some DT cord-319100-3gdawhfn 130 16 impact impact NN cord-319100-3gdawhfn 130 17 on on IN cord-319100-3gdawhfn 130 18 both both CC cord-319100-3gdawhfn 130 19 purified purify VBN cord-319100-3gdawhfn 130 20 DNA dna NN cord-319100-3gdawhfn 130 21 and and CC cord-319100-3gdawhfn 130 22 whole whole JJ cord-319100-3gdawhfn 130 23 virus virus NN cord-319100-3gdawhfn 130 24 . . . cord-319100-3gdawhfn 131 1 It -PRON- PRP cord-319100-3gdawhfn 131 2 is be VBZ cord-319100-3gdawhfn 131 3 also also RB cord-319100-3gdawhfn 131 4 important important JJ cord-319100-3gdawhfn 131 5 to to TO cord-319100-3gdawhfn 131 6 recognise recognise VB cord-319100-3gdawhfn 131 7 that that IN cord-319100-3gdawhfn 131 8 these these DT cord-319100-3gdawhfn 131 9 observations observation NNS cord-319100-3gdawhfn 131 10 reflect reflect VBP cord-319100-3gdawhfn 131 11 the the DT cord-319100-3gdawhfn 131 12 outcome outcome NN cord-319100-3gdawhfn 131 13 of of IN cord-319100-3gdawhfn 131 14 contact contact NN cord-319100-3gdawhfn 131 15 between between IN cord-319100-3gdawhfn 131 16 viral viral JJ cord-319100-3gdawhfn 131 17 nucleic nucleic JJ cord-319100-3gdawhfn 131 18 acid acid NN cord-319100-3gdawhfn 131 19 and and CC cord-319100-3gdawhfn 131 20 VTM VTM NNP cord-319100-3gdawhfn 131 21 for for IN cord-319100-3gdawhfn 131 22 less less JJR cord-319100-3gdawhfn 131 23 than than IN cord-319100-3gdawhfn 131 24 1 1 CD cord-319100-3gdawhfn 131 25 hour hour NN cord-319100-3gdawhfn 131 26 in in IN cord-319100-3gdawhfn 131 27 each each DT cord-319100-3gdawhfn 131 28 instance instance NN cord-319100-3gdawhfn 131 29 . . . cord-319100-3gdawhfn 132 1 The the DT cord-319100-3gdawhfn 132 2 levels level NNS cord-319100-3gdawhfn 132 3 of of IN cord-319100-3gdawhfn 132 4 nucleic nucleic JJ cord-319100-3gdawhfn 132 5 acid acid NN cord-319100-3gdawhfn 132 6 that that WDT cord-319100-3gdawhfn 132 7 were be VBD cord-319100-3gdawhfn 132 8 destroyed destroy VBN cord-319100-3gdawhfn 132 9 after after IN cord-319100-3gdawhfn 132 10 almost almost RB cord-319100-3gdawhfn 132 11 immediate immediate JJ cord-319100-3gdawhfn 132 12 addition addition NN cord-319100-3gdawhfn 132 13 to to IN cord-319100-3gdawhfn 132 14 the the DT cord-319100-3gdawhfn 132 15 VTMs vtm NNS cord-319100-3gdawhfn 132 16 were be VBD cord-319100-3gdawhfn 132 17 not not RB cord-319100-3gdawhfn 132 18 insignificant insignificant JJ cord-319100-3gdawhfn 132 19 . . . cord-319100-3gdawhfn 133 1 With with IN cord-319100-3gdawhfn 133 2 a a DT cord-319100-3gdawhfn 133 3 concentration concentration NN cord-319100-3gdawhfn 133 4 of of IN cord-319100-3gdawhfn 133 5 RNA RNA NNP cord-319100-3gdawhfn 133 6 that that WDT cord-319100-3gdawhfn 133 7 gave give VBD cord-319100-3gdawhfn 133 8 a a DT cord-319100-3gdawhfn 133 9 Ct Ct NNP cord-319100-3gdawhfn 133 10 value value NN cord-319100-3gdawhfn 133 11 of of IN cord-319100-3gdawhfn 133 12 approximately approximately RB cord-319100-3gdawhfn 133 13 21 21 CD cord-319100-3gdawhfn 133 14 in in IN cord-319100-3gdawhfn 133 15 both both CC cord-319100-3gdawhfn 133 16 PBS PBS NNP cord-319100-3gdawhfn 133 17 and and CC cord-319100-3gdawhfn 133 18 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 133 19 , , , cord-319100-3gdawhfn 133 20 this this DT cord-319100-3gdawhfn 133 21 represents represent VBZ cord-319100-3gdawhfn 133 22 a a DT cord-319100-3gdawhfn 133 23 reduction reduction NN cord-319100-3gdawhfn 133 24 of of IN cord-319100-3gdawhfn 133 25 approximately approximately RB cord-319100-3gdawhfn 133 26 ten ten CD cord-319100-3gdawhfn 133 27 thousand thousand CD cord-319100-3gdawhfn 133 28 - - HYPH cord-319100-3gdawhfn 133 29 fold fold NN cord-319100-3gdawhfn 133 30 and and CC cord-319100-3gdawhfn 133 31 can can MD cord-319100-3gdawhfn 133 32 not not RB cord-319100-3gdawhfn 133 33 be be VB cord-319100-3gdawhfn 133 34 ignored ignore VBN cord-319100-3gdawhfn 133 35 . . . cord-319100-3gdawhfn 134 1 While while IN cord-319100-3gdawhfn 134 2 it -PRON- PRP cord-319100-3gdawhfn 134 3 might may MD cord-319100-3gdawhfn 134 4 be be VB cord-319100-3gdawhfn 134 5 argued argue VBN cord-319100-3gdawhfn 134 6 that that IN cord-319100-3gdawhfn 134 7 the the DT cord-319100-3gdawhfn 134 8 adverse adverse JJ cord-319100-3gdawhfn 134 9 impact impact NN cord-319100-3gdawhfn 134 10 on on IN cord-319100-3gdawhfn 134 11 whole whole JJ cord-319100-3gdawhfn 134 12 virus virus NN cord-319100-3gdawhfn 134 13 appeared appear VBD cord-319100-3gdawhfn 134 14 to to TO cord-319100-3gdawhfn 134 15 be be VB cord-319100-3gdawhfn 134 16 slight slight JJ cord-319100-3gdawhfn 134 17 , , , cord-319100-3gdawhfn 134 18 free free JJ cord-319100-3gdawhfn 134 19 nucleic nucleic JJ cord-319100-3gdawhfn 134 20 acid acid NN cord-319100-3gdawhfn 134 21 and and CC cord-319100-3gdawhfn 134 22 perhaps perhaps RB cord-319100-3gdawhfn 134 23 whole whole JJ cord-319100-3gdawhfn 134 24 virus virus NN cord-319100-3gdawhfn 134 25 was be VBD cord-319100-3gdawhfn 134 26 destroyed destroy VBN cord-319100-3gdawhfn 134 27 at at IN cord-319100-3gdawhfn 134 28 levels level NNS cord-319100-3gdawhfn 134 29 that that WDT cord-319100-3gdawhfn 134 30 could could MD cord-319100-3gdawhfn 134 31 be be VB cord-319100-3gdawhfn 134 32 of of IN cord-319100-3gdawhfn 134 33 diagnostic diagnostic JJ cord-319100-3gdawhfn 134 34 relevance relevance NN cord-319100-3gdawhfn 134 35 ( ( -LRB- cord-319100-3gdawhfn 134 36 20 20 CD cord-319100-3gdawhfn 134 37 ) ) -RRB- cord-319100-3gdawhfn 134 38 . . . cord-319100-3gdawhfn 135 1 While while IN cord-319100-3gdawhfn 135 2 the the DT cord-319100-3gdawhfn 135 3 impact impact NN cord-319100-3gdawhfn 135 4 on on IN cord-319100-3gdawhfn 135 5 RNA RNA NNP cord-319100-3gdawhfn 135 6 viral viral JJ cord-319100-3gdawhfn 135 7 genomes genome NNS cord-319100-3gdawhfn 135 8 is be VBZ cord-319100-3gdawhfn 135 9 likely likely JJ cord-319100-3gdawhfn 135 10 to to TO cord-319100-3gdawhfn 135 11 be be VB cord-319100-3gdawhfn 135 12 markedly markedly RB cord-319100-3gdawhfn 135 13 greater great JJR cord-319100-3gdawhfn 135 14 than than IN cord-319100-3gdawhfn 135 15 on on IN cord-319100-3gdawhfn 135 16 DNA dna NN cord-319100-3gdawhfn 135 17 sequences sequence NNS cord-319100-3gdawhfn 135 18 , , , cord-319100-3gdawhfn 135 19 the the DT cord-319100-3gdawhfn 135 20 outcome outcome NN cord-319100-3gdawhfn 135 21 can can MD cord-319100-3gdawhfn 135 22 not not RB cord-319100-3gdawhfn 135 23 be be VB cord-319100-3gdawhfn 135 24 predicted predict VBN cord-319100-3gdawhfn 135 25 as as IN cord-319100-3gdawhfn 135 26 secondary secondary JJ cord-319100-3gdawhfn 135 27 structure structure NN cord-319100-3gdawhfn 135 28 may may MD cord-319100-3gdawhfn 135 29 also also RB cord-319100-3gdawhfn 135 30 have have VB cord-319100-3gdawhfn 135 31 an an DT cord-319100-3gdawhfn 135 32 influence influence NN cord-319100-3gdawhfn 135 33 ( ( -LRB- cord-319100-3gdawhfn 135 34 20 20 CD cord-319100-3gdawhfn 135 35 ) ) -RRB- cord-319100-3gdawhfn 135 36 and and CC cord-319100-3gdawhfn 135 37 the the DT cord-319100-3gdawhfn 135 38 speed speed NN cord-319100-3gdawhfn 135 39 and and CC cord-319100-3gdawhfn 135 40 severity severity NN cord-319100-3gdawhfn 135 41 of of IN cord-319100-3gdawhfn 135 42 the the DT cord-319100-3gdawhfn 135 43 impact impact NN cord-319100-3gdawhfn 135 44 may may MD cord-319100-3gdawhfn 135 45 vary vary VB cord-319100-3gdawhfn 135 46 depending depend VBG cord-319100-3gdawhfn 135 47 on on IN cord-319100-3gdawhfn 135 48 the the DT cord-319100-3gdawhfn 135 49 nucleic nucleic JJ cord-319100-3gdawhfn 135 50 acid acid NN cord-319100-3gdawhfn 135 51 target target NN cord-319100-3gdawhfn 135 52 , , , cord-319100-3gdawhfn 135 53 as as IN cord-319100-3gdawhfn 135 54 shown show VBN cord-319100-3gdawhfn 135 55 by by IN cord-319100-3gdawhfn 135 56 the the DT cord-319100-3gdawhfn 135 57 differences difference NNS cord-319100-3gdawhfn 135 58 between between IN cord-319100-3gdawhfn 135 59 the the DT cord-319100-3gdawhfn 135 60 results result NNS cord-319100-3gdawhfn 135 61 for for IN cord-319100-3gdawhfn 135 62 the the DT cord-319100-3gdawhfn 135 63 two two CD cord-319100-3gdawhfn 135 64 RNA RNA NNP cord-319100-3gdawhfn 135 65 viruses virus NNS cord-319100-3gdawhfn 135 66 and and CC cord-319100-3gdawhfn 135 67 the the DT cord-319100-3gdawhfn 135 68 XIPC XIPC NNP cord-319100-3gdawhfn 135 69 . . . cord-319100-3gdawhfn 136 1 Further further RB cord-319100-3gdawhfn 136 2 , , , cord-319100-3gdawhfn 136 3 it -PRON- PRP cord-319100-3gdawhfn 136 4 can can MD cord-319100-3gdawhfn 136 5 not not RB cord-319100-3gdawhfn 136 6 be be VB cord-319100-3gdawhfn 136 7 assumed assume VBN cord-319100-3gdawhfn 136 8 that that IN cord-319100-3gdawhfn 136 9 the the DT cord-319100-3gdawhfn 136 10 target target NN cord-319100-3gdawhfn 136 11 nucleic nucleic JJ cord-319100-3gdawhfn 136 12 acid acid NN cord-319100-3gdawhfn 136 13 will will MD cord-319100-3gdawhfn 136 14 always always RB cord-319100-3gdawhfn 136 15 be be VB cord-319100-3gdawhfn 136 16 protected protect VBN cord-319100-3gdawhfn 136 17 by by IN cord-319100-3gdawhfn 136 18 nucleoprotein nucleoprotein NNP cord-319100-3gdawhfn 136 19 . . . cord-319100-3gdawhfn 137 1 Degradation degradation NN cord-319100-3gdawhfn 137 2 will will MD cord-319100-3gdawhfn 137 3 occur occur VB cord-319100-3gdawhfn 137 4 during during IN cord-319100-3gdawhfn 137 5 the the DT cord-319100-3gdawhfn 137 6 course course NN cord-319100-3gdawhfn 137 7 of of IN cord-319100-3gdawhfn 137 8 an an DT cord-319100-3gdawhfn 137 9 infection infection NN cord-319100-3gdawhfn 137 10 and and CC cord-319100-3gdawhfn 137 11 also also RB cord-319100-3gdawhfn 137 12 under under IN cord-319100-3gdawhfn 137 13 conditions condition NNS cord-319100-3gdawhfn 137 14 where where WRB cord-319100-3gdawhfn 137 15 sample sample NN cord-319100-3gdawhfn 137 16 collection collection NN cord-319100-3gdawhfn 137 17 , , , cord-319100-3gdawhfn 137 18 transport transport NN cord-319100-3gdawhfn 137 19 and and CC cord-319100-3gdawhfn 137 20 storage storage NN cord-319100-3gdawhfn 137 21 are be VBP cord-319100-3gdawhfn 137 22 sub sub NN cord-319100-3gdawhfn 137 23 - - JJ cord-319100-3gdawhfn 137 24 optimal optimal JJ cord-319100-3gdawhfn 137 25 . . . cord-319100-3gdawhfn 138 1 Additionally additionally RB cord-319100-3gdawhfn 138 2 , , , cord-319100-3gdawhfn 138 3 the the DT cord-319100-3gdawhfn 138 4 adverse adverse JJ cord-319100-3gdawhfn 138 5 effects effect NNS cord-319100-3gdawhfn 138 6 observed observe VBN cord-319100-3gdawhfn 138 7 in in IN cord-319100-3gdawhfn 138 8 this this DT cord-319100-3gdawhfn 138 9 study study NN cord-319100-3gdawhfn 138 10 could could MD cord-319100-3gdawhfn 138 11 potentially potentially RB cord-319100-3gdawhfn 138 12 be be VB cord-319100-3gdawhfn 138 13 exacerbated exacerbate VBN cord-319100-3gdawhfn 138 14 with with IN cord-319100-3gdawhfn 138 15 alternative alternative JJ cord-319100-3gdawhfn 138 16 nucleic nucleic JJ cord-319100-3gdawhfn 138 17 acid acid NN cord-319100-3gdawhfn 138 18 purification purification NN cord-319100-3gdawhfn 138 19 technologies technology NNS cord-319100-3gdawhfn 138 20 that that WDT cord-319100-3gdawhfn 138 21 take take VBP cord-319100-3gdawhfn 138 22 longer long RBR cord-319100-3gdawhfn 138 23 than than IN cord-319100-3gdawhfn 138 24 the the DT cord-319100-3gdawhfn 138 25 20 20 CD cord-319100-3gdawhfn 138 26 minutes minute NNS cord-319100-3gdawhfn 138 27 required require VBN cord-319100-3gdawhfn 138 28 for for IN cord-319100-3gdawhfn 138 29 this this DT cord-319100-3gdawhfn 138 30 magnetic magnetic JJ cord-319100-3gdawhfn 138 31 - - HYPH cord-319100-3gdawhfn 138 32 bead bead NN cord-319100-3gdawhfn 138 33 based base VBN cord-319100-3gdawhfn 138 34 method method NN cord-319100-3gdawhfn 138 35 . . . cord-319100-3gdawhfn 139 1 While while IN cord-319100-3gdawhfn 139 2 undertaking undertake VBG cord-319100-3gdawhfn 139 3 surveillance surveillance NN cord-319100-3gdawhfn 139 4 and and CC cord-319100-3gdawhfn 139 5 epidemiological epidemiological JJ cord-319100-3gdawhfn 139 6 tracing tracing NN cord-319100-3gdawhfn 139 7 during during IN cord-319100-3gdawhfn 139 8 a a DT cord-319100-3gdawhfn 139 9 pandemic pandemic NN cord-319100-3gdawhfn 139 10 , , , cord-319100-3gdawhfn 139 11 failure failure NN cord-319100-3gdawhfn 139 12 to to TO cord-319100-3gdawhfn 139 13 detect detect VB cord-319100-3gdawhfn 139 14 a a DT cord-319100-3gdawhfn 139 15 moderate moderate JJ cord-319100-3gdawhfn 139 16 level level NN cord-319100-3gdawhfn 139 17 of of IN cord-319100-3gdawhfn 139 18 RNA RNA NNP cord-319100-3gdawhfn 139 19 in in IN cord-319100-3gdawhfn 139 20 a a DT cord-319100-3gdawhfn 139 21 person person NN cord-319100-3gdawhfn 139 22 who who WP cord-319100-3gdawhfn 139 23 is be VBZ cord-319100-3gdawhfn 139 24 asymptomatic asymptomatic JJ cord-319100-3gdawhfn 139 25 could could MD cord-319100-3gdawhfn 139 26 result result VB cord-319100-3gdawhfn 139 27 in in IN cord-319100-3gdawhfn 139 28 a a DT cord-319100-3gdawhfn 139 29 critical critical JJ cord-319100-3gdawhfn 139 30 source source NN cord-319100-3gdawhfn 139 31 of of IN cord-319100-3gdawhfn 139 32 infection infection NN cord-319100-3gdawhfn 139 33 remaining remain VBG cord-319100-3gdawhfn 139 34 undetected undetected JJ cord-319100-3gdawhfn 139 35 . . . cord-319100-3gdawhfn 140 1 However however RB cord-319100-3gdawhfn 140 2 , , , cord-319100-3gdawhfn 140 3 with with IN cord-319100-3gdawhfn 140 4 the the DT cord-319100-3gdawhfn 140 5 selection selection NN cord-319100-3gdawhfn 140 6 of of IN cord-319100-3gdawhfn 140 7 an an DT cord-319100-3gdawhfn 140 8 appropriate appropriate JJ cord-319100-3gdawhfn 140 9 transport transport NN cord-319100-3gdawhfn 140 10 medium medium NN cord-319100-3gdawhfn 140 11 , , , cord-319100-3gdawhfn 140 12 sample sample NN cord-319100-3gdawhfn 140 13 degradation degradation NN cord-319100-3gdawhfn 140 14 , , , cord-319100-3gdawhfn 140 15 even even RB cord-319100-3gdawhfn 140 16 at at IN cord-319100-3gdawhfn 140 17 room room NN cord-319100-3gdawhfn 140 18 temperature temperature NN cord-319100-3gdawhfn 140 19 , , , cord-319100-3gdawhfn 140 20 can can MD cord-319100-3gdawhfn 140 21 be be VB cord-319100-3gdawhfn 140 22 minimal minimal JJ cord-319100-3gdawhfn 140 23 . . . cord-319100-3gdawhfn 141 1 This this DT cord-319100-3gdawhfn 141 2 is be VBZ cord-319100-3gdawhfn 141 3 clearly clearly RB cord-319100-3gdawhfn 141 4 shown show VBN cord-319100-3gdawhfn 141 5 by by IN cord-319100-3gdawhfn 141 6 the the DT cord-319100-3gdawhfn 141 7 performance performance NN cord-319100-3gdawhfn 141 8 of of IN cord-319100-3gdawhfn 141 9 the the DT cord-319100-3gdawhfn 141 10 ' ' '' cord-319100-3gdawhfn 141 11 in in IN cord-319100-3gdawhfn 141 12 house house NN cord-319100-3gdawhfn 141 13 ' ' '' cord-319100-3gdawhfn 141 14 medium medium NN cord-319100-3gdawhfn 141 15 ( ( -LRB- cord-319100-3gdawhfn 141 16 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 141 17 ) ) -RRB- cord-319100-3gdawhfn 141 18 where where WRB cord-319100-3gdawhfn 141 19 there there EX cord-319100-3gdawhfn 141 20 is be VBZ cord-319100-3gdawhfn 141 21 little little JJ cord-319100-3gdawhfn 141 22 evidence evidence NN cord-319100-3gdawhfn 141 23 of of IN cord-319100-3gdawhfn 141 24 deterioration deterioration NN cord-319100-3gdawhfn 141 25 of of IN cord-319100-3gdawhfn 141 26 the the DT cord-319100-3gdawhfn 141 27 RNA RNA NNP cord-319100-3gdawhfn 141 28 samples sample NNS cord-319100-3gdawhfn 141 29 after after IN cord-319100-3gdawhfn 141 30 holding hold VBG cord-319100-3gdawhfn 141 31 at at IN cord-319100-3gdawhfn 141 32 room room NN cord-319100-3gdawhfn 141 33 temperature temperature NN cord-319100-3gdawhfn 141 34 for for IN cord-319100-3gdawhfn 141 35 2 2 CD cord-319100-3gdawhfn 141 36 days day NNS cord-319100-3gdawhfn 141 37 and and CC cord-319100-3gdawhfn 141 38 no no DT cord-319100-3gdawhfn 141 39 significant significant JJ cord-319100-3gdawhfn 141 40 deterioration deterioration NN cord-319100-3gdawhfn 141 41 of of IN cord-319100-3gdawhfn 141 42 virus virus NN cord-319100-3gdawhfn 141 43 when when WRB cord-319100-3gdawhfn 141 44 held hold VBN cord-319100-3gdawhfn 141 45 at at IN cord-319100-3gdawhfn 141 46 4 4 CD cord-319100-3gdawhfn 141 47 o o NN cord-319100-3gdawhfn 141 48 C c NN cord-319100-3gdawhfn 141 49 for for IN cord-319100-3gdawhfn 141 50 more more JJR cord-319100-3gdawhfn 141 51 than than IN cord-319100-3gdawhfn 141 52 6 6 CD cord-319100-3gdawhfn 141 53 weeks week NNS cord-319100-3gdawhfn 141 54 . . . cord-319100-3gdawhfn 142 1 The the DT cord-319100-3gdawhfn 142 2 World World NNP cord-319100-3gdawhfn 142 3 Health Health NNP cord-319100-3gdawhfn 142 4 Organisation Organisation NNP cord-319100-3gdawhfn 142 5 , , , cord-319100-3gdawhfn 142 6 the the DT cord-319100-3gdawhfn 142 7 US US NNP cord-319100-3gdawhfn 142 8 Centers Centers NNPS cord-319100-3gdawhfn 142 9 for for IN cord-319100-3gdawhfn 142 10 Disease Disease NNP cord-319100-3gdawhfn 142 11 Control Control NNP cord-319100-3gdawhfn 142 12 and and CC cord-319100-3gdawhfn 142 13 Prevention Prevention NNP cord-319100-3gdawhfn 142 14 ( ( -LRB- cord-319100-3gdawhfn 142 15 CDC CDC NNP cord-319100-3gdawhfn 142 16 ) ) -RRB- cord-319100-3gdawhfn 142 17 and and CC cord-319100-3gdawhfn 142 18 the the DT cord-319100-3gdawhfn 142 19 UK UK NNP cord-319100-3gdawhfn 142 20 government government NN cord-319100-3gdawhfn 142 21 have have VBP cord-319100-3gdawhfn 142 22 each each DT cord-319100-3gdawhfn 142 23 provided provide VBN cord-319100-3gdawhfn 142 24 recommendations recommendation NNS cord-319100-3gdawhfn 142 25 for for IN cord-319100-3gdawhfn 142 26 the the DT cord-319100-3gdawhfn 142 27 formulation formulation NN cord-319100-3gdawhfn 142 28 of of IN cord-319100-3gdawhfn 142 29 VTM VTM NNP cord-319100-3gdawhfn 142 30 solutions solution NNS cord-319100-3gdawhfn 142 31 to to TO cord-319100-3gdawhfn 142 32 be be VB cord-319100-3gdawhfn 142 33 used use VBN cord-319100-3gdawhfn 142 34 for for IN cord-319100-3gdawhfn 142 35 the the DT cord-319100-3gdawhfn 142 36 collection collection NN cord-319100-3gdawhfn 142 37 of of IN cord-319100-3gdawhfn 142 38 specimens specimen NNS cord-319100-3gdawhfn 142 39 for for IN cord-319100-3gdawhfn 142 40 SARS SARS NNP cord-319100-3gdawhfn 142 41 - - HYPH cord-319100-3gdawhfn 142 42 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 142 43 testing testing NN cord-319100-3gdawhfn 142 44 and and CC cord-319100-3gdawhfn 142 45 comment comment NN cord-319100-3gdawhfn 142 46 that that IN cord-319100-3gdawhfn 142 47 a a DT cord-319100-3gdawhfn 142 48 supplement supplement NN cord-319100-3gdawhfn 142 49 of of IN cord-319100-3gdawhfn 142 50 protein protein NN cord-319100-3gdawhfn 142 51 or or CC cord-319100-3gdawhfn 142 52 glycerol glycerol NN cord-319100-3gdawhfn 142 53 should should MD cord-319100-3gdawhfn 142 54 be be VB cord-319100-3gdawhfn 142 55 added add VBN cord-319100-3gdawhfn 142 56 to to TO cord-319100-3gdawhfn 142 57 enhance enhance VB cord-319100-3gdawhfn 142 58 the the DT cord-319100-3gdawhfn 142 59 stability stability NN cord-319100-3gdawhfn 142 60 of of IN cord-319100-3gdawhfn 142 61 viruses virus NNS cord-319100-3gdawhfn 142 62 ( ( -LRB- cord-319100-3gdawhfn 142 63 2 2 CD cord-319100-3gdawhfn 142 64 , , , cord-319100-3gdawhfn 142 65 3 3 CD cord-319100-3gdawhfn 142 66 , , , cord-319100-3gdawhfn 142 67 4 4 CD cord-319100-3gdawhfn 142 68 ) ) -RRB- cord-319100-3gdawhfn 142 69 . . . cord-319100-3gdawhfn 143 1 We -PRON- PRP cord-319100-3gdawhfn 143 2 believe believe VBP cord-319100-3gdawhfn 143 3 that that IN cord-319100-3gdawhfn 143 4 , , , cord-319100-3gdawhfn 143 5 while while IN cord-319100-3gdawhfn 143 6 this this DT cord-319100-3gdawhfn 143 7 is be VBZ cord-319100-3gdawhfn 143 8 an an DT cord-319100-3gdawhfn 143 9 essential essential JJ cord-319100-3gdawhfn 143 10 feature feature NN cord-319100-3gdawhfn 143 11 of of IN cord-319100-3gdawhfn 143 12 a a DT cord-319100-3gdawhfn 143 13 high high JJ cord-319100-3gdawhfn 143 14 quality quality NN cord-319100-3gdawhfn 143 15 VTM VTM NNP cord-319100-3gdawhfn 143 16 , , , cord-319100-3gdawhfn 143 17 it -PRON- PRP cord-319100-3gdawhfn 143 18 is be VBZ cord-319100-3gdawhfn 143 19 in in IN cord-319100-3gdawhfn 143 20 achieving achieve VBG cord-319100-3gdawhfn 143 21 this this DT cord-319100-3gdawhfn 143 22 requirement requirement NN cord-319100-3gdawhfn 143 23 that that IN cord-319100-3gdawhfn 143 24 the the DT cord-319100-3gdawhfn 143 25 current current JJ cord-319100-3gdawhfn 143 26 problem problem NN cord-319100-3gdawhfn 143 27 with with IN cord-319100-3gdawhfn 143 28 the the DT cord-319100-3gdawhfn 143 29 commercially commercially RB cord-319100-3gdawhfn 143 30 available available JJ cord-319100-3gdawhfn 143 31 VTMs vtm NNS cord-319100-3gdawhfn 143 32 may may MD cord-319100-3gdawhfn 143 33 have have VB cord-319100-3gdawhfn 143 34 arisen arise VBN cord-319100-3gdawhfn 143 35 . . . cord-319100-3gdawhfn 144 1 Our -PRON- PRP$ cord-319100-3gdawhfn 144 2 ' ' '' cord-319100-3gdawhfn 144 3 in in IN cord-319100-3gdawhfn 144 4 house house NN cord-319100-3gdawhfn 144 5 ' ' '' cord-319100-3gdawhfn 144 6 VTM VTM NNP cord-319100-3gdawhfn 144 7 includes include VBZ cord-319100-3gdawhfn 144 8 gelatin gelatin NN cord-319100-3gdawhfn 144 9 which which WDT cord-319100-3gdawhfn 144 10 is be VBZ cord-319100-3gdawhfn 144 11 extracted extract VBN cord-319100-3gdawhfn 144 12 from from IN cord-319100-3gdawhfn 144 13 animal animal NN cord-319100-3gdawhfn 144 14 tissues tissue NNS cord-319100-3gdawhfn 144 15 by by IN cord-319100-3gdawhfn 144 16 treatment treatment NN cord-319100-3gdawhfn 144 17 at at IN cord-319100-3gdawhfn 144 18 very very RB cord-319100-3gdawhfn 144 19 low low JJ cord-319100-3gdawhfn 144 20 or or CC cord-319100-3gdawhfn 144 21 high high JJ cord-319100-3gdawhfn 144 22 pH ph NN cord-319100-3gdawhfn 144 23 , , , cord-319100-3gdawhfn 144 24 and and CC cord-319100-3gdawhfn 144 25 prolonged prolong VBN cord-319100-3gdawhfn 144 26 boiling boiling NN cord-319100-3gdawhfn 144 27 at at IN cord-319100-3gdawhfn 144 28 high high JJ cord-319100-3gdawhfn 144 29 temperatures temperature NNS cord-319100-3gdawhfn 144 30 before before IN cord-319100-3gdawhfn 144 31 sterilisation sterilisation NN cord-319100-3gdawhfn 144 32 and and CC cord-319100-3gdawhfn 144 33 drying drying NN cord-319100-3gdawhfn 144 34 . . . cord-319100-3gdawhfn 145 1 These these DT cord-319100-3gdawhfn 145 2 steps step NNS cord-319100-3gdawhfn 145 3 inactivate inactivate VBP cord-319100-3gdawhfn 145 4 both both DT cord-319100-3gdawhfn 145 5 enzymes enzyme NNS cord-319100-3gdawhfn 145 6 and and CC cord-319100-3gdawhfn 145 7 infectious infectious JJ cord-319100-3gdawhfn 145 8 agents agent NNS cord-319100-3gdawhfn 145 9 that that WDT cord-319100-3gdawhfn 145 10 are be VBP cord-319100-3gdawhfn 145 11 present present JJ cord-319100-3gdawhfn 145 12 as as RB cord-319100-3gdawhfn 145 13 well well RB cord-319100-3gdawhfn 145 14 as as IN cord-319100-3gdawhfn 145 15 destroying destroy VBG cord-319100-3gdawhfn 145 16 residual residual JJ cord-319100-3gdawhfn 145 17 nucleic nucleic JJ cord-319100-3gdawhfn 145 18 acid acid NN cord-319100-3gdawhfn 145 19 . . . cord-319100-3gdawhfn 146 1 Further further RB cord-319100-3gdawhfn 146 2 , , , cord-319100-3gdawhfn 146 3 during during IN cord-319100-3gdawhfn 146 4 the the DT cord-319100-3gdawhfn 146 5 preparation preparation NN cord-319100-3gdawhfn 146 6 of of IN cord-319100-3gdawhfn 146 7 VTM-1 VTM-1 NNP cord-319100-3gdawhfn 146 8 , , , cord-319100-3gdawhfn 146 9 the the DT cord-319100-3gdawhfn 146 10 PBS PBS NNP cord-319100-3gdawhfn 146 11 solution solution NN cord-319100-3gdawhfn 146 12 to to TO cord-319100-3gdawhfn 146 13 which which WDT cord-319100-3gdawhfn 146 14 the the DT cord-319100-3gdawhfn 146 15 gelatin gelatin NN cord-319100-3gdawhfn 146 16 has have VBZ cord-319100-3gdawhfn 146 17 already already RB cord-319100-3gdawhfn 146 18 been be VBN cord-319100-3gdawhfn 146 19 added add VBN cord-319100-3gdawhfn 146 20 is be VBZ cord-319100-3gdawhfn 146 21 also also RB cord-319100-3gdawhfn 146 22 sterilised sterilise VBN cord-319100-3gdawhfn 146 23 by by IN cord-319100-3gdawhfn 146 24 heat heat NN cord-319100-3gdawhfn 146 25 treatment treatment NN cord-319100-3gdawhfn 146 26 . . . cord-319100-3gdawhfn 147 1 In in IN cord-319100-3gdawhfn 147 2 contrast contrast NN cord-319100-3gdawhfn 147 3 , , , cord-319100-3gdawhfn 147 4 products product NNS cord-319100-3gdawhfn 147 5 that that WDT cord-319100-3gdawhfn 147 6 include include VBP cord-319100-3gdawhfn 147 7 bovine bovine JJ cord-319100-3gdawhfn 147 8 serum serum NN cord-319100-3gdawhfn 147 9 albumen albumen NN cord-319100-3gdawhfn 147 10 or or CC cord-319100-3gdawhfn 147 11 other other JJ cord-319100-3gdawhfn 147 12 serum serum NN cord-319100-3gdawhfn 147 13 - - HYPH cord-319100-3gdawhfn 147 14 derived derive VBN cord-319100-3gdawhfn 147 15 components component NNS cord-319100-3gdawhfn 147 16 , , , cord-319100-3gdawhfn 147 17 such such JJ cord-319100-3gdawhfn 147 18 as as IN cord-319100-3gdawhfn 147 19 VTMs vtm NNS cord-319100-3gdawhfn 147 20 2 2 CD cord-319100-3gdawhfn 147 21 , , , cord-319100-3gdawhfn 147 22 3 3 CD cord-319100-3gdawhfn 147 23 and and CC cord-319100-3gdawhfn 147 24 4 4 CD cord-319100-3gdawhfn 147 25 , , , cord-319100-3gdawhfn 147 26 can can MD cord-319100-3gdawhfn 147 27 not not RB cord-319100-3gdawhfn 147 28 be be VB cord-319100-3gdawhfn 147 29 sterilised sterilise VBN cord-319100-3gdawhfn 147 30 by by IN cord-319100-3gdawhfn 147 31 autoclaving autoclave VBG cord-319100-3gdawhfn 147 32 without without IN cord-319100-3gdawhfn 147 33 coagulation coagulation NN cord-319100-3gdawhfn 147 34 of of IN cord-319100-3gdawhfn 147 35 the the DT cord-319100-3gdawhfn 147 36 protein protein NN cord-319100-3gdawhfn 147 37 supplement supplement NN cord-319100-3gdawhfn 147 38 . . . cord-319100-3gdawhfn 148 1 Therefore therefore RB cord-319100-3gdawhfn 148 2 , , , cord-319100-3gdawhfn 148 3 for for IN cord-319100-3gdawhfn 148 4 these these DT cord-319100-3gdawhfn 148 5 VTMs vtm NNS cord-319100-3gdawhfn 148 6 , , , cord-319100-3gdawhfn 148 7 the the DT cord-319100-3gdawhfn 148 8 raw raw JJ cord-319100-3gdawhfn 148 9 materials material NNS cord-319100-3gdawhfn 148 10 must must MD cord-319100-3gdawhfn 148 11 each each DT cord-319100-3gdawhfn 148 12 be be VB cord-319100-3gdawhfn 148 13 free free JJ cord-319100-3gdawhfn 148 14 of of IN cord-319100-3gdawhfn 148 15 nucleases nuclease NNS cord-319100-3gdawhfn 148 16 and and CC cord-319100-3gdawhfn 148 17 proteinases proteinase NNS cord-319100-3gdawhfn 148 18 prior prior RB cord-319100-3gdawhfn 148 19 to to IN cord-319100-3gdawhfn 148 20 sterilisation sterilisation NN cord-319100-3gdawhfn 148 21 by by IN cord-319100-3gdawhfn 148 22 methods method NNS cord-319100-3gdawhfn 148 23 that that WDT cord-319100-3gdawhfn 148 24 do do VBP cord-319100-3gdawhfn 148 25 not not RB cord-319100-3gdawhfn 148 26 include include VB cord-319100-3gdawhfn 148 27 heating heating NN cord-319100-3gdawhfn 148 28 . . . cord-319100-3gdawhfn 149 1 The the DT cord-319100-3gdawhfn 149 2 choice choice NN cord-319100-3gdawhfn 149 3 of of IN cord-319100-3gdawhfn 149 4 protein protein NN cord-319100-3gdawhfn 149 5 supplement supplement NN cord-319100-3gdawhfn 149 6 is be VBZ cord-319100-3gdawhfn 149 7 also also RB cord-319100-3gdawhfn 149 8 an an DT cord-319100-3gdawhfn 149 9 important important JJ cord-319100-3gdawhfn 149 10 consideration consideration NN cord-319100-3gdawhfn 149 11 from from IN cord-319100-3gdawhfn 149 12 the the DT cord-319100-3gdawhfn 149 13 perspective perspective NN cord-319100-3gdawhfn 149 14 of of IN cord-319100-3gdawhfn 149 15 inadvertent inadvertent JJ cord-319100-3gdawhfn 149 16 addition addition NN cord-319100-3gdawhfn 149 17 of of IN cord-319100-3gdawhfn 149 18 adventitious adventitious JJ cord-319100-3gdawhfn 149 19 agents agent NNS cord-319100-3gdawhfn 149 20 to to IN cord-319100-3gdawhfn 149 21 the the DT cord-319100-3gdawhfn 149 22 VTM VTM NNP cord-319100-3gdawhfn 149 23 and and CC cord-319100-3gdawhfn 149 24 perhaps perhaps RB cord-319100-3gdawhfn 149 25 genomic genomic JJ cord-319100-3gdawhfn 149 26 DNA dna NN cord-319100-3gdawhfn 149 27 from from IN cord-319100-3gdawhfn 149 28 animals animal NNS cord-319100-3gdawhfn 149 29 from from IN cord-319100-3gdawhfn 149 30 which which WDT cord-319100-3gdawhfn 149 31 the the DT cord-319100-3gdawhfn 149 32 protein protein NN cord-319100-3gdawhfn 149 33 supplement supplement NN cord-319100-3gdawhfn 149 34 has have VBZ cord-319100-3gdawhfn 149 35 been be VBN cord-319100-3gdawhfn 149 36 derived derive VBN cord-319100-3gdawhfn 149 37 . . . cord-319100-3gdawhfn 150 1 Foetal foetal JJ cord-319100-3gdawhfn 150 2 bovine bovine JJ cord-319100-3gdawhfn 150 3 serum serum NN cord-319100-3gdawhfn 150 4 ( ( -LRB- cord-319100-3gdawhfn 150 5 fbs fbs NNP cord-319100-3gdawhfn 150 6 ) ) -RRB- cord-319100-3gdawhfn 150 7 is be VBZ cord-319100-3gdawhfn 150 8 a a DT cord-319100-3gdawhfn 150 9 recommended recommend VBN cord-319100-3gdawhfn 150 10 supplement supplement NN cord-319100-3gdawhfn 150 11 ( ( -LRB- cord-319100-3gdawhfn 150 12 3 3 CD cord-319100-3gdawhfn 150 13 , , , cord-319100-3gdawhfn 150 14 4 4 CD cord-319100-3gdawhfn 150 15 ) ) -RRB- cord-319100-3gdawhfn 150 16 but but CC cord-319100-3gdawhfn 150 17 almost almost RB cord-319100-3gdawhfn 150 18 all all DT cord-319100-3gdawhfn 150 19 commercial commercial JJ cord-319100-3gdawhfn 150 20 batches batch NNS cord-319100-3gdawhfn 150 21 of of IN cord-319100-3gdawhfn 150 22 fbs fbs NN cord-319100-3gdawhfn 150 23 contain contain VBP cord-319100-3gdawhfn 150 24 pestiviruses pestiviruse NNS cord-319100-3gdawhfn 150 25 and and CC cord-319100-3gdawhfn 150 26 at at IN cord-319100-3gdawhfn 150 27 times time NNS cord-319100-3gdawhfn 150 28 other other JJ cord-319100-3gdawhfn 150 29 bovine bovine JJ cord-319100-3gdawhfn 150 30 viruses virus NNS cord-319100-3gdawhfn 150 31 . . . cord-319100-3gdawhfn 151 1 A a DT cord-319100-3gdawhfn 151 2 moderate moderate JJ cord-319100-3gdawhfn 151 3 concentration concentration NN cord-319100-3gdawhfn 151 4 of of IN cord-319100-3gdawhfn 151 5 BVDV BVDV NNP cord-319100-3gdawhfn 151 6 viral viral JJ cord-319100-3gdawhfn 151 7 RNA RNA NNP cord-319100-3gdawhfn 151 8 ( ( -LRB- cord-319100-3gdawhfn 151 9 Ct=30.6 Ct=30.6 NNP cord-319100-3gdawhfn 151 10 , , , cord-319100-3gdawhfn 151 11 data datum NNS cord-319100-3gdawhfn 151 12 not not RB cord-319100-3gdawhfn 151 13 included include VBN cord-319100-3gdawhfn 151 14 ) ) -RRB- cord-319100-3gdawhfn 151 15 was be VBD cord-319100-3gdawhfn 151 16 detected detect VBN cord-319100-3gdawhfn 151 17 in in IN cord-319100-3gdawhfn 151 18 VTM-4 VTM-4 NNP cord-319100-3gdawhfn 151 19 . . . cord-319100-3gdawhfn 152 1 As as IN cord-319100-3gdawhfn 152 2 whole whole JJ cord-319100-3gdawhfn 152 3 genome genome NN cord-319100-3gdawhfn 152 4 nucleic nucleic JJ cord-319100-3gdawhfn 152 5 acid acid NN cord-319100-3gdawhfn 152 6 sequencing sequencing NN cord-319100-3gdawhfn 152 7 is be VBZ cord-319100-3gdawhfn 152 8 now now RB cord-319100-3gdawhfn 152 9 often often RB cord-319100-3gdawhfn 152 10 undertaken undertake VBN cord-319100-3gdawhfn 152 11 on on IN cord-319100-3gdawhfn 152 12 many many JJ cord-319100-3gdawhfn 152 13 original original JJ cord-319100-3gdawhfn 152 14 , , , cord-319100-3gdawhfn 152 15 uncultured uncultured JJ cord-319100-3gdawhfn 152 16 patient patient NN cord-319100-3gdawhfn 152 17 samples sample NNS cord-319100-3gdawhfn 152 18 , , , cord-319100-3gdawhfn 152 19 the the DT cord-319100-3gdawhfn 152 20 presence presence NN cord-319100-3gdawhfn 152 21 of of IN cord-319100-3gdawhfn 152 22 these these DT cord-319100-3gdawhfn 152 23 viruses virus NNS cord-319100-3gdawhfn 152 24 and and CC cord-319100-3gdawhfn 152 25 other other JJ cord-319100-3gdawhfn 152 26 extraneous extraneous JJ cord-319100-3gdawhfn 152 27 nucleic nucleic JJ cord-319100-3gdawhfn 152 28 acid acid NN cord-319100-3gdawhfn 152 29 in in IN cord-319100-3gdawhfn 152 30 samples sample NNS cord-319100-3gdawhfn 152 31 may may MD cord-319100-3gdawhfn 152 32 reduce reduce VB cord-319100-3gdawhfn 152 33 the the DT cord-319100-3gdawhfn 152 34 sensitivity sensitivity NN cord-319100-3gdawhfn 152 35 of of IN cord-319100-3gdawhfn 152 36 sequencing sequencing NN cord-319100-3gdawhfn 152 37 protocols protocol NNS cord-319100-3gdawhfn 152 38 and and CC cord-319100-3gdawhfn 152 39 complicate complicate VB cord-319100-3gdawhfn 152 40 the the DT cord-319100-3gdawhfn 152 41 interpretation interpretation NN cord-319100-3gdawhfn 152 42 of of IN cord-319100-3gdawhfn 152 43 sequence sequence NN cord-319100-3gdawhfn 152 44 data datum NNS cord-319100-3gdawhfn 152 45 . . . cord-319100-3gdawhfn 153 1 The the DT cord-319100-3gdawhfn 153 2 nucleases nuclease NNS cord-319100-3gdawhfn 153 3 present present JJ cord-319100-3gdawhfn 153 4 may may MD cord-319100-3gdawhfn 153 5 also also RB cord-319100-3gdawhfn 153 6 have have VB cord-319100-3gdawhfn 153 7 an an DT cord-319100-3gdawhfn 153 8 impact impact NN cord-319100-3gdawhfn 153 9 on on IN cord-319100-3gdawhfn 153 10 the the DT cord-319100-3gdawhfn 153 11 quantity quantity NN cord-319100-3gdawhfn 153 12 of of IN cord-319100-3gdawhfn 153 13 RNA RNA NNP cord-319100-3gdawhfn 153 14 available available JJ cord-319100-3gdawhfn 153 15 for for IN cord-319100-3gdawhfn 153 16 sequencing sequencing NN cord-319100-3gdawhfn 153 17 . . . cord-319100-3gdawhfn 154 1 Furthermore furthermore RB cord-319100-3gdawhfn 154 2 , , , cord-319100-3gdawhfn 154 3 the the DT cord-319100-3gdawhfn 154 4 inclusion inclusion NN cord-319100-3gdawhfn 154 5 of of IN cord-319100-3gdawhfn 154 6 fbs fbs NNP cord-319100-3gdawhfn 154 7 presents present VBZ cord-319100-3gdawhfn 154 8 a a DT cord-319100-3gdawhfn 154 9 biosecurity biosecurity NN cord-319100-3gdawhfn 154 10 risk risk NN cord-319100-3gdawhfn 154 11 for for IN cord-319100-3gdawhfn 154 12 the the DT cord-319100-3gdawhfn 154 13 movement movement NN cord-319100-3gdawhfn 154 14 of of IN cord-319100-3gdawhfn 154 15 animal animal NN cord-319100-3gdawhfn 154 16 pathogens pathogen NNS cord-319100-3gdawhfn 154 17 between between IN cord-319100-3gdawhfn 154 18 countries country NNS cord-319100-3gdawhfn 154 19 and and CC cord-319100-3gdawhfn 154 20 renders render VBZ cord-319100-3gdawhfn 154 21 such such JJ cord-319100-3gdawhfn 154 22 VTM VTM NNP cord-319100-3gdawhfn 154 23 solutions solution NNS cord-319100-3gdawhfn 154 24 unsuitable unsuitable JJ cord-319100-3gdawhfn 154 25 for for IN cord-319100-3gdawhfn 154 26 many many JJ cord-319100-3gdawhfn 154 27 animal animal NN cord-319100-3gdawhfn 154 28 disease disease NN cord-319100-3gdawhfn 154 29 diagnostic diagnostic JJ cord-319100-3gdawhfn 154 30 or or CC cord-319100-3gdawhfn 154 31 research research NN cord-319100-3gdawhfn 154 32 applications application NNS cord-319100-3gdawhfn 154 33 . . . cord-319100-3gdawhfn 155 1 As as IN cord-319100-3gdawhfn 155 2 indicated indicate VBN cord-319100-3gdawhfn 155 3 in in IN cord-319100-3gdawhfn 155 4 the the DT cord-319100-3gdawhfn 155 5 UK UK NNP cord-319100-3gdawhfn 155 6 government government NN cord-319100-3gdawhfn 155 7 guideline guideline NN cord-319100-3gdawhfn 155 8 ( ( -LRB- cord-319100-3gdawhfn 155 9 4 4 LS cord-319100-3gdawhfn 155 10 ) ) -RRB- cord-319100-3gdawhfn 155 11 there there EX cord-319100-3gdawhfn 155 12 is be VBZ cord-319100-3gdawhfn 155 13 a a DT cord-319100-3gdawhfn 155 14 clear clear JJ cord-319100-3gdawhfn 155 15 need need NN cord-319100-3gdawhfn 155 16 for for IN cord-319100-3gdawhfn 155 17 the the DT cord-319100-3gdawhfn 155 18 " " `` cord-319100-3gdawhfn 155 19 Use Use NNP cord-319100-3gdawhfn 155 20 ( ( -LRB- cord-319100-3gdawhfn 155 21 of of IN cord-319100-3gdawhfn 155 22 ) ) -RRB- cord-319100-3gdawhfn 155 23 alternative alternative JJ cord-319100-3gdawhfn 155 24 swabs swab NNS cord-319100-3gdawhfn 155 25 and and CC cord-319100-3gdawhfn 155 26 transport transport NN cord-319100-3gdawhfn 155 27 medium medium NN cord-319100-3gdawhfn 155 28 in in IN cord-319100-3gdawhfn 155 29 accordance accordance NN cord-319100-3gdawhfn 155 30 with with IN cord-319100-3gdawhfn 155 31 a a DT cord-319100-3gdawhfn 155 32 locally locally RB cord-319100-3gdawhfn 155 33 validated validate VBN cord-319100-3gdawhfn 155 34 laboratory laboratory NN cord-319100-3gdawhfn 155 35 strategy strategy NN cord-319100-3gdawhfn 155 36 " " '' cord-319100-3gdawhfn 155 37 to to TO cord-319100-3gdawhfn 155 38 demonstrate demonstrate VB cord-319100-3gdawhfn 155 39 fitness fitness NN cord-319100-3gdawhfn 155 40 for for IN cord-319100-3gdawhfn 155 41 purpose purpose NN cord-319100-3gdawhfn 155 42 . . . cord-319100-3gdawhfn 156 1 The the DT cord-319100-3gdawhfn 156 2 specifications specification NNS cord-319100-3gdawhfn 156 3 for for IN cord-319100-3gdawhfn 156 4 products product NNS cord-319100-3gdawhfn 156 5 that that WDT cord-319100-3gdawhfn 156 6 might may MD cord-319100-3gdawhfn 156 7 be be VB cord-319100-3gdawhfn 156 8 used use VBN cord-319100-3gdawhfn 156 9 for for IN cord-319100-3gdawhfn 156 10 both both DT cord-319100-3gdawhfn 156 11 nucleic nucleic JJ cord-319100-3gdawhfn 156 12 acid acid NN cord-319100-3gdawhfn 156 13 detection detection NN cord-319100-3gdawhfn 156 14 methods method NNS cord-319100-3gdawhfn 156 15 and and CC cord-319100-3gdawhfn 156 16 virus virus NN cord-319100-3gdawhfn 156 17 culture culture NN cord-319100-3gdawhfn 156 18 are be VBP cord-319100-3gdawhfn 156 19 likely likely JJ cord-319100-3gdawhfn 156 20 to to TO cord-319100-3gdawhfn 156 21 be be VB cord-319100-3gdawhfn 156 22 more more RBR cord-319100-3gdawhfn 156 23 rigorous rigorous JJ cord-319100-3gdawhfn 156 24 than than IN cord-319100-3gdawhfn 156 25 for for IN cord-319100-3gdawhfn 156 26 those those DT cord-319100-3gdawhfn 156 27 VTMs vtm NNS cord-319100-3gdawhfn 156 28 that that WDT cord-319100-3gdawhfn 156 29 are be VBP cord-319100-3gdawhfn 156 30 only only RB cord-319100-3gdawhfn 156 31 used use VBN cord-319100-3gdawhfn 156 32 for for IN cord-319100-3gdawhfn 156 33 one one CD cord-319100-3gdawhfn 156 34 laboratory laboratory NN cord-319100-3gdawhfn 156 35 method method NN cord-319100-3gdawhfn 156 36 . . . cord-319100-3gdawhfn 157 1 The the DT cord-319100-3gdawhfn 157 2 special special JJ cord-319100-3gdawhfn 157 3 requirements requirement NNS cord-319100-3gdawhfn 157 4 for for IN cord-319100-3gdawhfn 157 5 products product NNS cord-319100-3gdawhfn 157 6 that that WDT cord-319100-3gdawhfn 157 7 are be VBP cord-319100-3gdawhfn 157 8 suitable suitable JJ cord-319100-3gdawhfn 157 9 for for IN cord-319100-3gdawhfn 157 10 nucleic nucleic JJ cord-319100-3gdawhfn 157 11 acid acid NN cord-319100-3gdawhfn 157 12 testing testing NN cord-319100-3gdawhfn 157 13 have have VBP cord-319100-3gdawhfn 157 14 been be VBN cord-319100-3gdawhfn 157 15 recognised recognise VBN cord-319100-3gdawhfn 157 16 by by IN cord-319100-3gdawhfn 157 17 some some DT cord-319100-3gdawhfn 157 18 manufacturers manufacturer NNS cord-319100-3gdawhfn 157 19 who who WP cord-319100-3gdawhfn 157 20 have have VBP cord-319100-3gdawhfn 157 21 developed develop VBN cord-319100-3gdawhfn 157 22 specific specific JJ cord-319100-3gdawhfn 157 23 transport transport NN cord-319100-3gdawhfn 157 24 media medium NNS cord-319100-3gdawhfn 157 25 to to TO cord-319100-3gdawhfn 157 26 inactivate inactivate VB cord-319100-3gdawhfn 157 27 the the DT cord-319100-3gdawhfn 157 28 viruses virus NNS cord-319100-3gdawhfn 157 29 of of IN cord-319100-3gdawhfn 157 30 interest interest NN cord-319100-3gdawhfn 157 31 and and CC cord-319100-3gdawhfn 157 32 to to TO cord-319100-3gdawhfn 157 33 minimise minimise VB cord-319100-3gdawhfn 157 34 the the DT cord-319100-3gdawhfn 157 35 degradation degradation NN cord-319100-3gdawhfn 157 36 of of IN cord-319100-3gdawhfn 157 37 nucleic nucleic JJ cord-319100-3gdawhfn 157 38 acid acid NN cord-319100-3gdawhfn 157 39 ( ( -LRB- cord-319100-3gdawhfn 157 40 10 10 CD cord-319100-3gdawhfn 157 41 , , , cord-319100-3gdawhfn 157 42 11 11 CD cord-319100-3gdawhfn 157 43 ) ) -RRB- cord-319100-3gdawhfn 157 44 . . . cord-319100-3gdawhfn 158 1 Some some DT cord-319100-3gdawhfn 158 2 of of IN cord-319100-3gdawhfn 158 3 the the DT cord-319100-3gdawhfn 158 4 major major JJ cord-319100-3gdawhfn 158 5 manufacturers manufacturer NNS cord-319100-3gdawhfn 158 6 of of IN cord-319100-3gdawhfn 158 7 VTM VTM NNP cord-319100-3gdawhfn 158 8 solutions solution NNS cord-319100-3gdawhfn 158 9 also also RB cord-319100-3gdawhfn 158 10 offer offer VBP cord-319100-3gdawhfn 158 11 products product NNS cord-319100-3gdawhfn 158 12 with with IN cord-319100-3gdawhfn 158 13 additives additive NNS cord-319100-3gdawhfn 158 14 to to TO cord-319100-3gdawhfn 158 15 reduce reduce VB cord-319100-3gdawhfn 158 16 nuclease nuclease NN cord-319100-3gdawhfn 158 17 activity activity NN cord-319100-3gdawhfn 158 18 but but CC cord-319100-3gdawhfn 158 19 most most JJS cord-319100-3gdawhfn 158 20 of of IN cord-319100-3gdawhfn 158 21 these these DT cord-319100-3gdawhfn 158 22 also also RB cord-319100-3gdawhfn 158 23 preclude preclude VBP cord-319100-3gdawhfn 158 24 opportunities opportunity NNS cord-319100-3gdawhfn 158 25 to to TO cord-319100-3gdawhfn 158 26 undertake undertake VB cord-319100-3gdawhfn 158 27 virus virus NN cord-319100-3gdawhfn 158 28 culture culture NN cord-319100-3gdawhfn 158 29 . . . cord-319100-3gdawhfn 159 1 However however RB cord-319100-3gdawhfn 159 2 , , , cord-319100-3gdawhfn 159 3 these these DT cord-319100-3gdawhfn 159 4 limitations limitation NNS cord-319100-3gdawhfn 159 5 are be VBP cord-319100-3gdawhfn 159 6 often often RB cord-319100-3gdawhfn 159 7 not not RB cord-319100-3gdawhfn 159 8 apparent apparent JJ cord-319100-3gdawhfn 159 9 to to IN cord-319100-3gdawhfn 159 10 purchasing purchase VBG cord-319100-3gdawhfn 159 11 departments department NNS cord-319100-3gdawhfn 159 12 , , , cord-319100-3gdawhfn 159 13 especially especially RB cord-319100-3gdawhfn 159 14 during during IN cord-319100-3gdawhfn 159 15 a a DT cord-319100-3gdawhfn 159 16 pandemic pandemic NN cord-319100-3gdawhfn 159 17 , , , cord-319100-3gdawhfn 159 18 when when WRB cord-319100-3gdawhfn 159 19 any any DT cord-319100-3gdawhfn 159 20 VTM VTM NNP cord-319100-3gdawhfn 159 21 may may MD cord-319100-3gdawhfn 159 22 be be VB cord-319100-3gdawhfn 159 23 mistakenly mistakenly RB cord-319100-3gdawhfn 159 24 thought think VBN cord-319100-3gdawhfn 159 25 to to TO cord-319100-3gdawhfn 159 26 be be VB cord-319100-3gdawhfn 159 27 " " `` cord-319100-3gdawhfn 159 28 fit fit JJ cord-319100-3gdawhfn 159 29 for for IN cord-319100-3gdawhfn 159 30 purpose purpose NN cord-319100-3gdawhfn 159 31 " " '' cord-319100-3gdawhfn 159 32 . . . cord-319100-3gdawhfn 160 1 In in IN cord-319100-3gdawhfn 160 2 conclusion conclusion NN cord-319100-3gdawhfn 160 3 , , , cord-319100-3gdawhfn 160 4 the the DT cord-319100-3gdawhfn 160 5 results result NNS cord-319100-3gdawhfn 160 6 of of IN cord-319100-3gdawhfn 160 7 this this DT cord-319100-3gdawhfn 160 8 study study NN cord-319100-3gdawhfn 160 9 provide provide VBP cord-319100-3gdawhfn 160 10 examples example NNS cord-319100-3gdawhfn 160 11 of of IN cord-319100-3gdawhfn 160 12 how how WRB cord-319100-3gdawhfn 160 13 the the DT cord-319100-3gdawhfn 160 14 composition composition NN cord-319100-3gdawhfn 160 15 of of IN cord-319100-3gdawhfn 160 16 a a DT cord-319100-3gdawhfn 160 17 VTM VTM NNP cord-319100-3gdawhfn 160 18 could could MD cord-319100-3gdawhfn 160 19 have have VB cord-319100-3gdawhfn 160 20 an an DT cord-319100-3gdawhfn 160 21 impact impact NN cord-319100-3gdawhfn 160 22 on on IN cord-319100-3gdawhfn 160 23 the the DT cord-319100-3gdawhfn 160 24 outcome outcome NN cord-319100-3gdawhfn 160 25 of of IN cord-319100-3gdawhfn 160 26 nucleic nucleic JJ cord-319100-3gdawhfn 160 27 acid acid NN cord-319100-3gdawhfn 160 28 based base VBN cord-319100-3gdawhfn 160 29 testing testing NN cord-319100-3gdawhfn 160 30 and and CC cord-319100-3gdawhfn 160 31 , , , cord-319100-3gdawhfn 160 32 in in IN cord-319100-3gdawhfn 160 33 particular particular JJ cord-319100-3gdawhfn 160 34 , , , cord-319100-3gdawhfn 160 35 situations situation NNS cord-319100-3gdawhfn 160 36 where where WRB cord-319100-3gdawhfn 160 37 either either CC cord-319100-3gdawhfn 160 38 there there EX cord-319100-3gdawhfn 160 39 is be VBZ cord-319100-3gdawhfn 160 40 a a DT cord-319100-3gdawhfn 160 41 need need NN cord-319100-3gdawhfn 160 42 to to TO cord-319100-3gdawhfn 160 43 detect detect VB cord-319100-3gdawhfn 160 44 RNA RNA NNP cord-319100-3gdawhfn 160 45 that that WDT cord-319100-3gdawhfn 160 46 is be VBZ cord-319100-3gdawhfn 160 47 not not RB cord-319100-3gdawhfn 160 48 packaged package VBN cord-319100-3gdawhfn 160 49 into into IN cord-319100-3gdawhfn 160 50 a a DT cord-319100-3gdawhfn 160 51 nucleocapsid nucleocapsid NN cord-319100-3gdawhfn 160 52 or or CC cord-319100-3gdawhfn 160 53 where where WRB cord-319100-3gdawhfn 160 54 RNA RNA NNP cord-319100-3gdawhfn 160 55 constructs construct NNS cord-319100-3gdawhfn 160 56 may may MD cord-319100-3gdawhfn 160 57 be be VB cord-319100-3gdawhfn 160 58 diluted dilute VBN cord-319100-3gdawhfn 160 59 in in IN cord-319100-3gdawhfn 160 60 a a DT cord-319100-3gdawhfn 160 61 VTM VTM NNP cord-319100-3gdawhfn 160 62 for for IN cord-319100-3gdawhfn 160 63 use use NN cord-319100-3gdawhfn 160 64 as as IN cord-319100-3gdawhfn 160 65 a a DT cord-319100-3gdawhfn 160 66 positive positive JJ cord-319100-3gdawhfn 160 67 control control NN cord-319100-3gdawhfn 160 68 in in IN cord-319100-3gdawhfn 160 69 an an DT cord-319100-3gdawhfn 160 70 assay assay NN cord-319100-3gdawhfn 160 71 or or CC cord-319100-3gdawhfn 160 72 perhaps perhaps RB cord-319100-3gdawhfn 160 73 for for IN cord-319100-3gdawhfn 160 74 proficiency proficiency NN cord-319100-3gdawhfn 160 75 testing testing NN cord-319100-3gdawhfn 160 76 . . . cord-319100-3gdawhfn 161 1 Finally finally RB cord-319100-3gdawhfn 161 2 , , , cord-319100-3gdawhfn 161 3 and and CC cord-319100-3gdawhfn 161 4 particularly particularly RB cord-319100-3gdawhfn 161 5 in in IN cord-319100-3gdawhfn 161 6 the the DT cord-319100-3gdawhfn 161 7 face face NN cord-319100-3gdawhfn 161 8 of of IN cord-319100-3gdawhfn 161 9 a a DT cord-319100-3gdawhfn 161 10 pandemic pandemic NN cord-319100-3gdawhfn 161 11 , , , cord-319100-3gdawhfn 161 12 users user NNS cord-319100-3gdawhfn 161 13 should should MD cord-319100-3gdawhfn 161 14 be be VB cord-319100-3gdawhfn 161 15 reminded remind VBN cord-319100-3gdawhfn 161 16 that that IN cord-319100-3gdawhfn 161 17 products product NNS cord-319100-3gdawhfn 161 18 fit fit JJ cord-319100-3gdawhfn 161 19 for for IN cord-319100-3gdawhfn 161 20 one one CD cord-319100-3gdawhfn 161 21 purpose purpose NN cord-319100-3gdawhfn 161 22 may may MD cord-319100-3gdawhfn 161 23 not not RB cord-319100-3gdawhfn 161 24 be be VB cord-319100-3gdawhfn 161 25 suitable suitable JJ cord-319100-3gdawhfn 161 26 for for IN cord-319100-3gdawhfn 161 27 an an DT cord-319100-3gdawhfn 161 28 alternative alternative JJ cord-319100-3gdawhfn 161 29 use use NN cord-319100-3gdawhfn 161 30 . . . cord-319100-3gdawhfn 162 1 A a DT cord-319100-3gdawhfn 162 2 product product NN cord-319100-3gdawhfn 162 3 that that WDT cord-319100-3gdawhfn 162 4 may may MD cord-319100-3gdawhfn 162 5 be be VB cord-319100-3gdawhfn 162 6 eminently eminently RB cord-319100-3gdawhfn 162 7 suitable suitable JJ cord-319100-3gdawhfn 162 8 for for IN cord-319100-3gdawhfn 162 9 virus virus NN cord-319100-3gdawhfn 162 10 culture culture NN cord-319100-3gdawhfn 162 11 purposes purpose NNS cord-319100-3gdawhfn 162 12 could could MD cord-319100-3gdawhfn 162 13 result result VB cord-319100-3gdawhfn 162 14 in in IN cord-319100-3gdawhfn 162 15 misleading misleading JJ cord-319100-3gdawhfn 162 16 results result NNS cord-319100-3gdawhfn 162 17 if if IN cord-319100-3gdawhfn 162 18 used use VBN cord-319100-3gdawhfn 162 19 for for IN cord-319100-3gdawhfn 162 20 nucleic nucleic JJ cord-319100-3gdawhfn 162 21 acid acid NN cord-319100-3gdawhfn 162 22 - - HYPH cord-319100-3gdawhfn 162 23 based base VBN cord-319100-3gdawhfn 162 24 tests test NNS cord-319100-3gdawhfn 162 25 . . . cord-319100-3gdawhfn 163 1 and and CC cord-319100-3gdawhfn 163 2 other other JJ cord-319100-3gdawhfn 163 3 staff staff NN cord-319100-3gdawhfn 163 4 of of IN cord-319100-3gdawhfn 163 5 the the DT cord-319100-3gdawhfn 163 6 Virology Virology NNP cord-319100-3gdawhfn 163 7 Laboratory Laboratory NNP cord-319100-3gdawhfn 163 8 at at IN cord-319100-3gdawhfn 163 9 EMAI EMAI NNP cord-319100-3gdawhfn 163 10 for for IN cord-319100-3gdawhfn 163 11 their -PRON- PRP$ cord-319100-3gdawhfn 163 12 assistance assistance NN cord-319100-3gdawhfn 163 13 during during IN cord-319100-3gdawhfn 163 14 the the DT cord-319100-3gdawhfn 163 15 preparation preparation NN cord-319100-3gdawhfn 163 16 of of IN cord-319100-3gdawhfn 163 17 samples sample NNS cord-319100-3gdawhfn 163 18 for for IN cord-319100-3gdawhfn 163 19 the the DT cord-319100-3gdawhfn 163 20 initial initial JJ cord-319100-3gdawhfn 163 21 experiment experiment NN cord-319100-3gdawhfn 163 22 and and CC cord-319100-3gdawhfn 163 23 the the DT cord-319100-3gdawhfn 163 24 longer long JJR cord-319100-3gdawhfn 163 25 - - HYPH cord-319100-3gdawhfn 163 26 term term NN cord-319100-3gdawhfn 163 27 storage storage NN cord-319100-3gdawhfn 163 28 of of IN cord-319100-3gdawhfn 163 29 swabs swab NNS cord-319100-3gdawhfn 163 30 . . . cord-319100-3gdawhfn 164 1 WHO who WP cord-319100-3gdawhfn 164 2 Manual Manual NNP cord-319100-3gdawhfn 164 3 on on IN cord-319100-3gdawhfn 164 4 Animal Animal NNP cord-319100-3gdawhfn 164 5 Influenza Influenza NNP cord-319100-3gdawhfn 164 6 Surveillance Surveillance NNP cord-319100-3gdawhfn 164 7 and and CC cord-319100-3gdawhfn 164 8 Diagnosis Diagnosis NNP cord-319100-3gdawhfn 164 9 Collecting Collecting NNP cord-319100-3gdawhfn 164 10 , , , cord-319100-3gdawhfn 164 11 preserving preserve VBG cord-319100-3gdawhfn 164 12 and and CC cord-319100-3gdawhfn 164 13 shipping shipping NN cord-319100-3gdawhfn 164 14 specimens specimen NNS cord-319100-3gdawhfn 164 15 for for IN cord-319100-3gdawhfn 164 16 the the DT cord-319100-3gdawhfn 164 17 diagnosis diagnosis NN cord-319100-3gdawhfn 164 18 of of IN cord-319100-3gdawhfn 164 19 avian avian JJ cord-319100-3gdawhfn 164 20 influenza influenza NN cord-319100-3gdawhfn 164 21 A(H5N1 A(H5N1 NNP cord-319100-3gdawhfn 164 22 ) ) -RRB- cord-319100-3gdawhfn 164 23 virus virus NN cord-319100-3gdawhfn 164 24 infection infection NN cord-319100-3gdawhfn 164 25 Centers Centers NNPS cord-319100-3gdawhfn 164 26 for for IN cord-319100-3gdawhfn 164 27 Disease Disease NNP cord-319100-3gdawhfn 164 28 Control Control NNP cord-319100-3gdawhfn 164 29 and and CC cord-319100-3gdawhfn 164 30 Prevention Prevention NNP cord-319100-3gdawhfn 164 31 , , , cord-319100-3gdawhfn 164 32 2020 2020 CD cord-319100-3gdawhfn 164 33 . . . cord-319100-3gdawhfn 165 1 Preparation preparation NN cord-319100-3gdawhfn 165 2 of of IN cord-319100-3gdawhfn 165 3 Viral Viral NNP cord-319100-3gdawhfn 165 4 Transport Transport NNP cord-319100-3gdawhfn 165 5 Medium Medium NNP cord-319100-3gdawhfn 165 6 . . . cord-319100-3gdawhfn 166 1 Standard Standard NNP cord-319100-3gdawhfn 166 2 Operating Operating NNP cord-319100-3gdawhfn 166 3 Procedure Procedure NNP cord-319100-3gdawhfn 166 4 SOP sop NN cord-319100-3gdawhfn 166 5 # # NN cord-319100-3gdawhfn 166 6 COVID-19 covid-19 NN cord-319100-3gdawhfn 166 7 : : : cord-319100-3gdawhfn 166 8 guidance guidance NN cord-319100-3gdawhfn 166 9 for for IN cord-319100-3gdawhfn 166 10 sampling sampling NN cord-319100-3gdawhfn 166 11 and and CC cord-319100-3gdawhfn 166 12 for for IN cord-319100-3gdawhfn 166 13 diagnostic diagnostic JJ cord-319100-3gdawhfn 166 14 laboratories laboratory NNS cord-319100-3gdawhfn 166 15 Maintenance Maintenance NNP cord-319100-3gdawhfn 166 16 of of IN cord-319100-3gdawhfn 166 17 viability viability NN cord-319100-3gdawhfn 166 18 and and CC cord-319100-3gdawhfn 166 19 comparison comparison NN cord-319100-3gdawhfn 166 20 of of IN cord-319100-3gdawhfn 166 21 identification identification NN cord-319100-3gdawhfn 166 22 methods method NNS cord-319100-3gdawhfn 166 23 for for IN cord-319100-3gdawhfn 166 24 influenza influenza NN cord-319100-3gdawhfn 166 25 and and CC cord-319100-3gdawhfn 166 26 other other JJ cord-319100-3gdawhfn 166 27 respiratory respiratory JJ cord-319100-3gdawhfn 166 28 viruses virus NNS cord-319100-3gdawhfn 166 29 of of IN cord-319100-3gdawhfn 166 30 humans human NNS cord-319100-3gdawhfn 166 31 Comparison comparison NN cord-319100-3gdawhfn 166 32 of of IN cord-319100-3gdawhfn 166 33 various various JJ cord-319100-3gdawhfn 166 34 transport transport NN cord-319100-3gdawhfn 166 35 media medium NNS cord-319100-3gdawhfn 166 36 for for IN cord-319100-3gdawhfn 166 37 viability viability NN cord-319100-3gdawhfn 166 38 maintenance maintenance NN cord-319100-3gdawhfn 166 39 of of IN cord-319100-3gdawhfn 166 40 herpes herpes NN cord-319100-3gdawhfn 166 41 simplex simplex NNP cord-319100-3gdawhfn 166 42 virus virus NN cord-319100-3gdawhfn 166 43 , , , cord-319100-3gdawhfn 166 44 respiratory respiratory JJ cord-319100-3gdawhfn 166 45 syncytial syncytial JJ cord-319100-3gdawhfn 166 46 virus virus NN cord-319100-3gdawhfn 166 47 and and CC cord-319100-3gdawhfn 166 48 adenovirus adenovirus NN cord-319100-3gdawhfn 166 49 Evaluation Evaluation NNP cord-319100-3gdawhfn 166 50 of of IN cord-319100-3gdawhfn 166 51 Swabs swab NNS cord-319100-3gdawhfn 166 52 , , , cord-319100-3gdawhfn 166 53 Transport transport NN cord-319100-3gdawhfn 166 54 media medium NNS cord-319100-3gdawhfn 166 55 and and CC cord-319100-3gdawhfn 166 56 specimen specimen JJ cord-319100-3gdawhfn 166 57 transport transport NN cord-319100-3gdawhfn 166 58 conditions condition NNS cord-319100-3gdawhfn 166 59 for for IN cord-319100-3gdawhfn 166 60 optimal optimal JJ cord-319100-3gdawhfn 166 61 detection detection NN cord-319100-3gdawhfn 166 62 of of IN cord-319100-3gdawhfn 166 63 viruses virus NNS cord-319100-3gdawhfn 166 64 by by IN cord-319100-3gdawhfn 166 65 PCR PCR NNP cord-319100-3gdawhfn 166 66 Comparison Comparison NNP cord-319100-3gdawhfn 166 67 of of IN cord-319100-3gdawhfn 166 68 nasopharyngeal nasopharyngeal JJ cord-319100-3gdawhfn 166 69 nylon nylon NN cord-319100-3gdawhfn 166 70 flocked flock VBD cord-319100-3gdawhfn 166 71 swabs swab NNS cord-319100-3gdawhfn 166 72 with with IN cord-319100-3gdawhfn 166 73 universal universal JJ cord-319100-3gdawhfn 166 74 transport transport NN cord-319100-3gdawhfn 166 75 medium medium NN cord-319100-3gdawhfn 166 76 and and CC cord-319100-3gdawhfn 166 77 rayonbud rayonbud JJ cord-319100-3gdawhfn 166 78 swabs swab NNS cord-319100-3gdawhfn 166 79 with with IN cord-319100-3gdawhfn 166 80 a a DT cord-319100-3gdawhfn 166 81 sponge sponge NN cord-319100-3gdawhfn 166 82 reservoir reservoir NN cord-319100-3gdawhfn 166 83 of of IN cord-319100-3gdawhfn 166 84 viral viral JJ cord-319100-3gdawhfn 166 85 transport transport NN cord-319100-3gdawhfn 166 86 medium medium NN cord-319100-3gdawhfn 166 87 in in IN cord-319100-3gdawhfn 166 88 the the DT cord-319100-3gdawhfn 166 89 diagnosis diagnosis NN cord-319100-3gdawhfn 166 90 of of IN cord-319100-3gdawhfn 166 91 paediatric paediatric JJ cord-319100-3gdawhfn 166 92 influenza influenza NN cord-319100-3gdawhfn 166 93 Comparison comparison NN cord-319100-3gdawhfn 166 94 of of IN cord-319100-3gdawhfn 166 95 Copan Copan NNP cord-319100-3gdawhfn 166 96 Eswab Eswab NNP cord-319100-3gdawhfn 166 97 and and CC cord-319100-3gdawhfn 166 98 FLOQswab FLOQswab NNP cord-319100-3gdawhfn 166 99 for for IN cord-319100-3gdawhfn 166 100 COVID-19 COVID-19 NNP cord-319100-3gdawhfn 166 101 PCR PCR NNP cord-319100-3gdawhfn 166 102 diagnosis diagnosis NN cord-319100-3gdawhfn 166 103 : : : cord-319100-3gdawhfn 166 104 working work VBG cord-319100-3gdawhfn 166 105 around around IN cord-319100-3gdawhfn 166 106 a a DT cord-319100-3gdawhfn 166 107 supply supply NN cord-319100-3gdawhfn 166 108 shortage shortage NN cord-319100-3gdawhfn 166 109 A a DT cord-319100-3gdawhfn 166 110 clinical clinical JJ cord-319100-3gdawhfn 166 111 specimen specimen NN cord-319100-3gdawhfn 166 112 collection collection NN cord-319100-3gdawhfn 166 113 and and CC cord-319100-3gdawhfn 166 114 transport transport NN cord-319100-3gdawhfn 166 115 medium medium NN cord-319100-3gdawhfn 166 116 for for IN cord-319100-3gdawhfn 166 117 molecular molecular JJ cord-319100-3gdawhfn 166 118 diagnostic diagnostic JJ cord-319100-3gdawhfn 166 119 and and CC cord-319100-3gdawhfn 166 120 genomic genomic JJ cord-319100-3gdawhfn 166 121 applications application NNS cord-319100-3gdawhfn 166 122 Comparison comparison NN cord-319100-3gdawhfn 166 123 of of IN cord-319100-3gdawhfn 166 124 a a DT cord-319100-3gdawhfn 166 125 new new JJ cord-319100-3gdawhfn 166 126 transport transport NN cord-319100-3gdawhfn 166 127 medium medium NN cord-319100-3gdawhfn 166 128 with with IN cord-319100-3gdawhfn 166 129 universal universal JJ cord-319100-3gdawhfn 166 130 transport transport NN cord-319100-3gdawhfn 166 131 medium medium NN cord-319100-3gdawhfn 166 132 at at IN cord-319100-3gdawhfn 166 133 a a DT cord-319100-3gdawhfn 166 134 tropical tropical JJ cord-319100-3gdawhfn 166 135 field field NN cord-319100-3gdawhfn 166 136 site site NN cord-319100-3gdawhfn 166 137 Influenza Influenza NNP cord-319100-3gdawhfn 166 138 Virus Virus NNP cord-319100-3gdawhfn 166 139 A A NNP cord-319100-3gdawhfn 166 140 ( ( -LRB- cord-319100-3gdawhfn 166 141 H10N7 h10n7 RB cord-319100-3gdawhfn 166 142 ) ) -RRB- cord-319100-3gdawhfn 166 143 in in IN cord-319100-3gdawhfn 166 144 Chickens Chickens NNPS cord-319100-3gdawhfn 166 145 and and CC cord-319100-3gdawhfn 166 146 Poultry Poultry NNP cord-319100-3gdawhfn 166 147 Abattoir Abattoir NNP cord-319100-3gdawhfn 166 148 Workers Workers NNPS cord-319100-3gdawhfn 166 149 Protocol Protocol NNP cord-319100-3gdawhfn 166 150 : : : cord-319100-3gdawhfn 166 151 Real real JJ cord-319100-3gdawhfn 166 152 - - HYPH cord-319100-3gdawhfn 166 153 time time NN cord-319100-3gdawhfn 166 154 RT rt NN cord-319100-3gdawhfn 166 155 - - HYPH cord-319100-3gdawhfn 166 156 PCR PCR NNP cord-319100-3gdawhfn 166 157 assays assay NNS cord-319100-3gdawhfn 166 158 for for IN cord-319100-3gdawhfn 166 159 the the DT cord-319100-3gdawhfn 166 160 detection detection NN cord-319100-3gdawhfn 166 161 of of IN cord-319100-3gdawhfn 166 162 SARS SARS NNP cord-319100-3gdawhfn 166 163 - - HYPH cord-319100-3gdawhfn 166 164 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 166 165 Institut Institut NNP cord-319100-3gdawhfn 166 166 Pasteur Pasteur NNP cord-319100-3gdawhfn 166 167 Development Development NNP cord-319100-3gdawhfn 166 168 and and CC cord-319100-3gdawhfn 166 169 performance performance NN cord-319100-3gdawhfn 166 170 evaluation evaluation NN cord-319100-3gdawhfn 166 171 of of IN cord-319100-3gdawhfn 166 172 calf calf NN cord-319100-3gdawhfn 166 173 diarrhea diarrhea NN cord-319100-3gdawhfn 166 174 pathogen pathogen NN cord-319100-3gdawhfn 166 175 nucleic nucleic VBZ cord-319100-3gdawhfn 166 176 acid acid NN cord-319100-3gdawhfn 166 177 purification purification NN cord-319100-3gdawhfn 166 178 and and CC cord-319100-3gdawhfn 166 179 detection detection NN cord-319100-3gdawhfn 166 180 workflow workflow NN cord-319100-3gdawhfn 166 181 Centers Centers NNPS cord-319100-3gdawhfn 166 182 for for IN cord-319100-3gdawhfn 166 183 Disease Disease NNP cord-319100-3gdawhfn 166 184 Control Control NNP cord-319100-3gdawhfn 166 185 and and CC cord-319100-3gdawhfn 166 186 Prevention Prevention NNP cord-319100-3gdawhfn 166 187 , , , cord-319100-3gdawhfn 166 188 2020 2020 CD cord-319100-3gdawhfn 166 189 . . . cord-319100-3gdawhfn 167 1 2019-Novel 2019-novel CD cord-319100-3gdawhfn 167 2 Coronavirus Coronavirus NNP cord-319100-3gdawhfn 167 3 ( ( -LRB- cord-319100-3gdawhfn 167 4 2019-nCoV 2019-ncov CD cord-319100-3gdawhfn 167 5 ) ) -RRB- cord-319100-3gdawhfn 167 6 Real real JJ cord-319100-3gdawhfn 167 7 - - HYPH cord-319100-3gdawhfn 167 8 time time NN cord-319100-3gdawhfn 167 9 rRT rrt NN cord-319100-3gdawhfn 167 10 - - HYPH cord-319100-3gdawhfn 167 11 PCR PCR NNP cord-319100-3gdawhfn 167 12 Panel Panel NNP cord-319100-3gdawhfn 167 13 Primers Primers NNPS cord-319100-3gdawhfn 167 14 and and CC cord-319100-3gdawhfn 167 15 Probes Probes NNPS cord-319100-3gdawhfn 167 16 Rapid Rapid NNP cord-319100-3gdawhfn 167 17 detection detection NN cord-319100-3gdawhfn 167 18 of of IN cord-319100-3gdawhfn 167 19 highly highly RB cord-319100-3gdawhfn 167 20 pathogenic pathogenic JJ cord-319100-3gdawhfn 167 21 avian avian JJ cord-319100-3gdawhfn 167 22 influenza influenza NN cord-319100-3gdawhfn 167 23 H5N1 H5N1 NNP cord-319100-3gdawhfn 167 24 virus virus NN cord-319100-3gdawhfn 167 25 by by IN cord-319100-3gdawhfn 167 26 TaqMan TaqMan NNP cord-319100-3gdawhfn 167 27 reverse reverse JJ cord-319100-3gdawhfn 167 28 transcriptase transcriptase NN cord-319100-3gdawhfn 167 29 - - HYPH cord-319100-3gdawhfn 167 30 polymerase polymerase NN cord-319100-3gdawhfn 167 31 chain chain NN cord-319100-3gdawhfn 167 32 reaction reaction NN cord-319100-3gdawhfn 167 33 Validation validation NN cord-319100-3gdawhfn 167 34 of of IN cord-319100-3gdawhfn 167 35 a a DT cord-319100-3gdawhfn 167 36 real real JJ cord-319100-3gdawhfn 167 37 - - HYPH cord-319100-3gdawhfn 167 38 time time NN cord-319100-3gdawhfn 167 39 PCR pcr NN cord-319100-3gdawhfn 167 40 assay assay NN cord-319100-3gdawhfn 167 41 for for IN cord-319100-3gdawhfn 167 42 the the DT cord-319100-3gdawhfn 167 43 detection detection NN cord-319100-3gdawhfn 167 44 of of IN cord-319100-3gdawhfn 167 45 bovine bovine JJ cord-319100-3gdawhfn 167 46 herpesvirus herpesvirus NN cord-319100-3gdawhfn 167 47 1 1 CD cord-319100-3gdawhfn 167 48 in in IN cord-319100-3gdawhfn 167 49 bovine bovine JJ cord-319100-3gdawhfn 167 50 semen semen NN cord-319100-3gdawhfn 167 51 A a DT cord-319100-3gdawhfn 167 52 universal universal JJ cord-319100-3gdawhfn 167 53 heterologous heterologous JJ cord-319100-3gdawhfn 167 54 internal internal JJ cord-319100-3gdawhfn 167 55 control control NN cord-319100-3gdawhfn 167 56 system system NN cord-319100-3gdawhfn 167 57 for for IN cord-319100-3gdawhfn 167 58 duplex duplex JJ cord-319100-3gdawhfn 167 59 real real JJ cord-319100-3gdawhfn 167 60 - - HYPH cord-319100-3gdawhfn 167 61 time time NN cord-319100-3gdawhfn 167 62 RT RT NNP cord-319100-3gdawhfn 167 63 - - HYPH cord-319100-3gdawhfn 167 64 PCR PCR NNP cord-319100-3gdawhfn 167 65 assays assay NNS cord-319100-3gdawhfn 167 66 used use VBN cord-319100-3gdawhfn 167 67 in in IN cord-319100-3gdawhfn 167 68 a a DT cord-319100-3gdawhfn 167 69 detection detection NN cord-319100-3gdawhfn 167 70 system system NN cord-319100-3gdawhfn 167 71 for for IN cord-319100-3gdawhfn 167 72 pestiviruses pestiviruses NNP cord-319100-3gdawhfn 167 73 Impact Impact NNP cord-319100-3gdawhfn 167 74 of of IN cord-319100-3gdawhfn 167 75 RNA RNA NNP cord-319100-3gdawhfn 167 76 Degradation Degradation NNP cord-319100-3gdawhfn 167 77 on on IN cord-319100-3gdawhfn 167 78 Viral Viral NNP cord-319100-3gdawhfn 167 79 Diagnosis diagnosis NN cord-319100-3gdawhfn 167 80 : : : cord-319100-3gdawhfn 168 1 An an DT cord-319100-3gdawhfn 168 2 Understated understated JJ cord-319100-3gdawhfn 168 3 but but CC cord-319100-3gdawhfn 168 4 Essential Essential NNP cord-319100-3gdawhfn 168 5 Step Step NNP cord-319100-3gdawhfn 168 6 for for IN cord-319100-3gdawhfn 168 7 the the DT cord-319100-3gdawhfn 168 8 Successful Successful NNP cord-319100-3gdawhfn 168 9 Establishment Establishment NNP cord-319100-3gdawhfn 168 10 of of IN cord-319100-3gdawhfn 168 11 a a DT cord-319100-3gdawhfn 168 12 Diagnosis Diagnosis NNP cord-319100-3gdawhfn 168 13 Network Network NNP cord-319100-3gdawhfn 169 1 The the DT cord-319100-3gdawhfn 169 2 authors author NNS cord-319100-3gdawhfn 169 3 are be VBP cord-319100-3gdawhfn 169 4 indebted indebted JJ cord-319100-3gdawhfn 169 5 to to IN cord-319100-3gdawhfn 169 6 Mr Mr NNP cord-319100-3gdawhfn 169 7 Ian Ian NNP cord-319100-3gdawhfn 169 8 Carter Carter NNP cord-319100-3gdawhfn 169 9 , , , cord-319100-3gdawhfn 169 10 Institute Institute NNP cord-319100-3gdawhfn 169 11 of of IN cord-319100-3gdawhfn 169 12 Clinical Clinical NNP cord-319100-3gdawhfn 169 13 Pathology Pathology NNP cord-319100-3gdawhfn 169 14 and and CC cord-319100-3gdawhfn 169 15 Medical Medical NNP cord-319100-3gdawhfn 169 16 Research Research NNP cord-319100-3gdawhfn 169 17 , , , cord-319100-3gdawhfn 169 18 Westmead Westmead NNP cord-319100-3gdawhfn 169 19 Hospital Hospital NNP cord-319100-3gdawhfn 169 20 , , , cord-319100-3gdawhfn 169 21 Westmead Westmead NNP cord-319100-3gdawhfn 169 22 NSW NSW NNP cord-319100-3gdawhfn 169 23 for for IN cord-319100-3gdawhfn 169 24 the the DT cord-319100-3gdawhfn 169 25 generous generous JJ cord-319100-3gdawhfn 169 26 supply supply NN cord-319100-3gdawhfn 169 27 of of IN cord-319100-3gdawhfn 169 28 the the DT cord-319100-3gdawhfn 169 29 purified purify VBN cord-319100-3gdawhfn 169 30 SARS SARS NNP cord-319100-3gdawhfn 169 31 - - HYPH cord-319100-3gdawhfn 169 32 CoV-2 CoV-2 NNP cord-319100-3gdawhfn 169 33 RNA RNA NNP cord-319100-3gdawhfn 169 34 and and CC cord-319100-3gdawhfn 169 35 for for IN cord-319100-3gdawhfn 169 36 referral referral NN cord-319100-3gdawhfn 169 37 of of IN cord-319100-3gdawhfn 169 38 the the DT cord-319100-3gdawhfn 169 39 patient patient JJ cord-319100-3gdawhfn 169 40 sample sample NN cord-319100-3gdawhfn 169 41 . . . cord-319100-3gdawhfn 170 1 We -PRON- PRP cord-319100-3gdawhfn 170 2 also also RB cord-319100-3gdawhfn 170 3 appreciate appreciate VBP cord-319100-3gdawhfn 170 4 the the DT cord-319100-3gdawhfn 170 5 productive productive JJ cord-319100-3gdawhfn 170 6 discussions discussion NNS cord-319100-3gdawhfn 170 7 with with IN cord-319100-3gdawhfn 170 8 Drs Drs NNP cord-319100-3gdawhfn 170 9 Catherine Catherine NNP cord-319100-3gdawhfn 170 10 Pitman Pitman NNP cord-319100-3gdawhfn 170 11 and and CC cord-319100-3gdawhfn 170 12 Dominic Dominic NNP cord-319100-3gdawhfn 170 13 Dwyer Dwyer NNP cord-319100-3gdawhfn 170 14 , , , cord-319100-3gdawhfn 170 15 NSW NSW NNP cord-319100-3gdawhfn 170 16 Health Health NNP cord-319100-3gdawhfn 170 17 Pathology Pathology NNP cord-319100-3gdawhfn 170 18 regarding regard VBG cord-319100-3gdawhfn 170 19 the the DT cord-319100-3gdawhfn 170 20 need need NN cord-319100-3gdawhfn 170 21 for for IN cord-319100-3gdawhfn 170 22 rigorous rigorous JJ cord-319100-3gdawhfn 170 23 evaluation evaluation NN cord-319100-3gdawhfn 170 24 of of IN cord-319100-3gdawhfn 170 25 viral viral JJ cord-319100-3gdawhfn 170 26 transport transport NN cord-319100-3gdawhfn 170 27 media medium NNS cord-319100-3gdawhfn 170 28 . . . cord-319100-3gdawhfn 171 1 We -PRON- PRP cord-319100-3gdawhfn 171 2 also also RB cord-319100-3gdawhfn 171 3 thank thank VBP cord-319100-3gdawhfn 171 4 Dr Dr NNP cord-319100-3gdawhfn 171 5 Deb Deb NNP cord-319100-3gdawhfn 171 6 Finlaison Finlaison NNP cord-319100-3gdawhfn 171 7 for for IN cord-319100-3gdawhfn 171 8 helpful helpful JJ cord-319100-3gdawhfn 171 9 comments comment NNS cord-319100-3gdawhfn 171 10 on on IN cord-319100-3gdawhfn 171 11 the the DT cord-319100-3gdawhfn 171 12 draft draft NN cord-319100-3gdawhfn 171 13 manuscript manuscript NN cord-319100-3gdawhfn 171 14 and and CC cord-319100-3gdawhfn 171 15 Shannon Shannon NNP cord-319100-3gdawhfn 171 16 Mollica Mollica NNP cord-319100-3gdawhfn 171 17 , , , cord-319100-3gdawhfn 171 18 Rodney Rodney NNP cord-319100-3gdawhfn 171 19 Davis Davis NNP