id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-268527-wbfnhedy	Smith, Sylvia B.	Transient hyperglycosylation of rhodopsin with galactose	1991-10-31		.txt	text/plain	8560	468	58	Following subcellular fractionation of retinas, ConA purified rhodopsin from ROS was applied to one of two additional lectin columns: Ricinus communis agglutinin (RCA) or Griffonia simplicifolia I (GSA). To radiolabel existing galactose moieties on rhodopsin, bovine ROS purified by a discontinuous sucrose density gradient procedure, were subjected to enzymatic treatment with galactose oxidase followed by a reduction with tritium labeled sodium borohydride. In this method, ConA purified rhodopsin or in some cases the clarified extract of ROS from six rat retinas labeled with [35S]methionine was applied to columns of either Ricinus communis agglutinin I (RCA) or GrifSonia simplicijolia I (GSA), specific for P-linked or a-linked galactose residues, respectively. Rats injected intravitreally with [35S]methionine were maintained in darkness for 2 hr at which time 12 retinas were collected for subcellular fractionation and rhodopsin purification by ConA sepharose chromatography.	./cache/cord-268527-wbfnhedy.txt	./txt/cord-268527-wbfnhedy.txt