key: cord-259968-cr3zf4oa
authors: Harb, Roa; Remaley, Alan T; Sacks, David B
title: Evaluation of Three Commercial Automated Assays for the Detection of anti-SARS-CoV-2 Antibodies
date: 2020-08-06
journal: Clin Chem
DOI: 10.1093/clinchem/hvaa193
sha: 
doc_id: 259968
cord_uid: cr3zf4oa

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serum to calibrator (S/C). The Diasorin assay detects IgG antibodies to the S1 and S2 subunits of the spike protein and the signal is expressed in arbitrary units (AU/mL). The Roche assay detects total antibodies to the nucleocapsid protein and the signal is reported as a cutoff index (COI). Cutoffs for positive samples by the Abbott, Diasorin, and Roche assays are ≥ 1.4, ≥ 15 AU/mL, and ≥ 1.0 COI, respectively.

The distribution of anti-SARS-CoV-2 results is presented in Figure 1A 

Clinical performance of two sars-cov-2 serologic assays

Clinical performance of the roche sars-cov-2 serologic assay

Clinical performance of the elecsys electrochemiluminescent immunoassay for the detection of sars-cov-2 total antibodies

Performance characteristics of four high-throughput immunoassays for detection of igg antibodies against sars-cov-2

Validation of a chemiluminescent assay for specific sars-cov-2 antibody

IgG for expected negative (n=336) and expected positive (n=64) specimens. Middle, values for Diasorin SARS-CoV-2 IgG for expected negative (n=344) and expected positive (n=65) specimens. Right, values for Roche SARS-CoV-2 total antibodies for expected negative (n=141) and expected positive (n=65) specimens. B) Distribution of anti-SARS-CoV-2 antibody results in expected positive specimens at 0-11 (n=12)

S/C: serum/calibrator; AU: arbitrary units; COI: cutoff index