key: cord-319184-voc0eqb9 authors: Abduljalil, Jameel M. title: Laboratory diagnosis of SARS-CoV-2: available approaches and limitations date: 2020-06-14 journal: New Microbes New Infect DOI: 10.1016/j.nmni.2020.100713 sha: doc_id: 319184 cord_uid: voc0eqb9 Abstract The ongoing pandemic of SARS-CoV-2 is a one of the most devastating outbreaks witnessed in the last 100 years. The outbreak started in China's hinterland and spread rapidly to almost every country culminating in woefully overwhelmed healthcare systems in most countries. The only approved diagnostic test to accompany radiographic evaluation is the reverse-transcriptase PCR. However, the applicability of this test in diagnosis and surveillance is challenged by global shortage in reagents and unavailability of well-equipped laboratories with specialized staff in several low- and middle-income countries. The need for development of accurate and rapid diagnostic assays became apparent. Handful of immunodiagnostic tests and other molecular approaches were developed and tested. Other recently developed point-of-care molecular tests are expected to be helpful in pandemic management since no particular skills are required from the operator. Fortunately, handful of serological tests have granted authorization to be used under emergency situation by FDA in diagnosis of SARS-CoV-2. Medically important coronaviruses were identified from nasal secretions of patients with mild RdRp/Hel (RNA-dependent RNA polymerase/helicase). Of note, a recent study found 146 nucleocapsid N2 and envelope E genes to be the most sensitive singleplex reactions and no 147 significant change in cycle threshold (Ct) was noted when both assays were combined [44] . The This work is a personal effort and did not receive any specific grant from funding agencies in the 269 public, commercial, or not-for-profit sectors. Author declares the absence of any potential or actual conflict of interest. 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