id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256608-ajzk86rq	van Weezep, Erik	PCR diagnostics: In silico validation by an automated tool using freely available software programs	2019-05-13		.txt	text/plain	4950	258	54	An alignment search was performed with the default expectancy threshold value on all fasta files using primers and probes of the PCR test as search queries and the program SSEARCH available in the FASTA sequence analysis package (Brenner et al., 1998; Pearson, 1991; Pearson et al., 2017; . The in silico specificity is expressed as the percentage of specific hits of taxonomy classified sequences with a maximum of one mismatch per primer or probe as these are considered to be detected with the respective PCR test. To demonstrate the suitability of our in-house developed software tool PCRv, we determined the in silico sensitivity and specificity of three PCR tests for West Nile virus (WNV) recommended by the World Organisation for Animal Health (OIE) (Eiden et al., 2010; Johnson et al., 2001) .	./cache/cord-256608-ajzk86rq.txt	./txt/cord-256608-ajzk86rq.txt