id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267269-05mezubh	Plazolles, N.	Pivotal Advance: The promotion of soluble DC‐SIGN release by inflammatory signals and its enhancement of cytomegalovirus‐mediated cis‐infection of myeloid dendritic cells	2010-10-12		.txt	text/plain	8815	452	53	Previous studies have reported that TM-lacking encoding DC-SIGN sequences are transcribed as soluble cytoplasmic pro-teins but not secreted by the sDC-SIGN-expressing transfectant cells or immature MoDCs [19] . Thus, using our quantitative and specific ELISA, we observed the presence of potential sDC-SIGN isoforms in 10ϫ concentrated cell culture supernatants of mDC-SIGN-expressing DCs. In vitro DCs were generated from adult monocytes or CD34 ϩ CBPs, according to already well-known protocols [17, 23, 30] . Despite the use of high concentrations of Marimastat (10 M), no modification of sDC-SIGN versus mDC-SIGN expression patterns could be observed, thus weighing in favor of a sliced, sequence-derived product and not a shedding of mDC-SIGN by MMPs. Like many cell types, DCs are able to secrete 60 -80 nm membrane vesicles, called exosomes. In addition, as mDC-SIGN expression was reported to be responsible for MoDC CMV cis-infection, we assumed that FLAG-sDC-SIGN1AT1 may function as a promoter of the infection.	./cache/cord-267269-05mezubh.txt	./txt/cord-267269-05mezubh.txt