id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287799-ridm3qd7	Martínez, María Guadalupe	S-layer proteins of Lactobacillus acidophilus inhibits JUNV infection	2012-06-15		.txt	text/plain	3547	175	51	Among those, Junin virus (JUNV) entry is enhanced in cells expressing DC-SIGN and for that reason surface-layer protein (S-layer) of Lactobacillus acidophilus ATCC 4365 was evaluated as a possible JUNV inhibitor. Experiments using 3T3 cells stably expressing DC-SIGN, showed an almost complete inhibition of JUNV infection when they were treated with S-layer in a similar extend as the inhibition shown by mannan. Since 3T3 cells are poorly infect by JUNV in the absence of expression of C-type lectins this model provides a really strong tool to study the effect of S-layer protein on the infection enhanced by DC-SIGN or L-SIGN. We presumed that the C-terminal portion of the S-layer would be responsible of the interaction with the glycan strand of DC-SIGN, since we have already shown it to interact with cell wall peptidoglycan [3] and it has been reported, that two repeats sequences in the C-terminal portion of the protein showed homology to carbohydrate binding domains (CBD) of Clostridium difficile S-layer/ toxin [27, 28] .	./cache/cord-287799-ridm3qd7.txt	./txt/cord-287799-ridm3qd7.txt