id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010260-8lnpujip	Anthonsen, Henrik W.	The blind watchmaker and rational protein engineering	1994-08-31		.txt	text/plain	17317	916	49	In the present review some scientific areas of key importance for protein engineering are discussed, such as problems involved in deducting protein sequence from DNA sequence (due to posttranscriptional editing, splicing and posttranslational modifications), modelling of protein structures by homology, NMR of large proteins (including probing the molecular surface with relaxation agents), simulation of protein structures by molecular dynamics and simulation of electrostatic effects in proteins (including pH-dependent effects). In the present review we will look at the design part of the protein engineering process, with emphasis on some of the more difficult steps, especially homology based modelling in cases with very low sequence similarity, nuclear magnetic resonance (NMR) of very large proteins and modelling of electrostatic interactions. They modify properties of individual residues and of the protein, and may thus make surface prediction, dynamics simulations and structural modelling in general more complex.	./cache/cord-010260-8lnpujip.txt	./txt/cord-010260-8lnpujip.txt