id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305378-jmcuq9c5	Chen, Hui	Liquid–liquid phase separation by SARS-CoV-2 nucleocapsid protein and RNA	2020-09-08		.txt	text/plain	527	32	61	title: Liquid–liquid phase separation by SARS-CoV-2 nucleocapsid protein and RNA well refolded proteins were concentrated and further purified with anion exchange chromatography column and size exclusion chromatography. The N protein RNA N1541 (1541 nt; Supplementary information, Table S1 ) and A85 H20 (20 nt), A20 (20 nt) and poly-U RNA oligos of different lengths (U10, U20, U40, U60; Supplementary information, Table S1) with HEX labeling were purchased from Sangon Biotech (Shanghai China). All RNA sequences were listed in Supplementary information, Table S1 . Phase separation was induced with RNA addition and then transferred in 96-well plate at room temperature for 1 h. Samples were prepared by mixing indicated concentration of protein and RNA. The particle size of N protein was detected by a DynaPro NanoStar (Wyatt Technology) which was equipped with a 660 nm laser, detector angle was fixed at 90°.	./cache/cord-305378-jmcuq9c5.txt	./txt/cord-305378-jmcuq9c5.txt