id author title date pages extension mime words sentences flesch summary cache txt cord-004729-nmkilkcx Reynolds, D. J. Virus isolation and serum antibody responses after infection of cats with transmissible gastroenteritis virus 1979 .txt text/plain 1998 135 54 No clinical disease resulted but infectious virus was isolated from faeces for up to 22 days after infection and serum antibody was detected by neutralisation and immunofluorescence tests. No clinical disease resulted but infectious virus was isolated from faeces for up to 22 days after infection and serum antibody was detected by neutralisation and immunofluorescence tests. Control and infected animals were tested for TGEV excretion by attempted isolation of virus from faeces for up to 35 days after infection; occasionally rectal swabs were used to obtain fresh samples. The medium was changed after 24 hours and 4 days later any viral eytopathic effect was recorded, the medium was removed and passaged in fresh APT/2 cultures and the eoverslips were stained to demonstrate virM antigen by indirect immunofluorescence, using paired sera from a gnotobiotic piglet before and after TGEV infection, followed by FITCconjugated rabbit anti-swine globulin (Nordic Immunological Laboratories, London). ./cache/cord-004729-nmkilkcx.txt ./txt/cord-004729-nmkilkcx.txt