id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287266-sd5izamc	Song, Zhenhui	EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication	2019-04-30		.txt	text/plain	4398	242	54	title: EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication Using the GST pull-down approach and a CO-IP assay, the M protein was found to interact with porcine intestinal cells via eukaryotic translation initiation factor 4-alpha (EIF4A2), an essential component of the cellular translational machinery. The present study provides mechanistic insight into the interaction between the TGEV M protein and intestinal cells which contributes to the understanding of coronavirus replication and may be useful for the development of novel therapeutic strategies for TGEV infection. In the present study, using yeast two-hybrid screening, seven cellular proteins, including eukaryotic translation initiation factor 4-alpha (EIF4A2), were identified to be M-ligands. Moreover, previous reports have shown that EIF4A2 interacts with VP1 of infectious bursal disease virus (IBD) and inhibits the RNA polymerase activity of IBDV to reduce its replication in host cells (Tacken et al., 2004; Gao et al., 2017) .	./cache/cord-287266-sd5izamc.txt	./txt/cord-287266-sd5izamc.txt