id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309433-wm0k13qh	Paton, D. J.	An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus	1991-08-31		.txt	text/plain	1036	59	56	Abstract A competition ELISA utilizing a mAb directed towards a peplomer protein epitope common to TGEV, PRCV and related feline and canine coronaviruses is described. Serological differentiation between TGEV and PRCV is now possible using competition ELISAs and monoclonal antibodies (mAbs) to TGEV-specific epitopes (Callebaut el al., 1989 , van Nieuwstadt & Boonstra, 1990 . This communication describes a competition ELISA utilizing a mAb (6A .C3) directed towards a peplomer protein epitope common to TGEV, PRCV, and a number of related feline and canine coronaviruses, but not to other porcine coronaviruses (Sanchez et al., 1990) . VNT positive sera from field cases (n=167) and from pigs experimentally infected with TGEV or PRCV (n=35) all scored positive in ELISA (values : 1-49%) . A recent serological survey of British pigs by TGEV-specific ELISA indicated a seroprevalence of only 0 .6% (Brown & Paton, 1991) , suggesting that most TGEV/PRCV antibody positive samples reflect PRCV infection and that such infections remain common .	./cache/cord-309433-wm0k13qh.txt	./txt/cord-309433-wm0k13qh.txt