id sid tid token lemma pos cord-269867-k10siwur 1 1 key key JJ cord-269867-k10siwur 1 2 : : : cord-269867-k10siwur 1 3 cord-269867-k10siwur cord-269867-k10siwur NN cord-269867-k10siwur 1 4 authors author NNS cord-269867-k10siwur 1 5 : : : cord-269867-k10siwur 1 6 Méchin Méchin NNP cord-269867-k10siwur 1 7 , , , cord-269867-k10siwur 1 8 Marie Marie NNP cord-269867-k10siwur 1 9 - - HYPH cord-269867-k10siwur 1 10 Claire Claire NNP cord-269867-k10siwur 1 11 ; ; : cord-269867-k10siwur 1 12 Der Der NNP cord-269867-k10siwur 1 13 Vartanian Vartanian NNP cord-269867-k10siwur 1 14 , , , cord-269867-k10siwur 1 15 Maurice Maurice NNP cord-269867-k10siwur 1 16 ; ; : cord-269867-k10siwur 1 17 Martin Martin NNP cord-269867-k10siwur 1 18 , , , cord-269867-k10siwur 1 19 Christine Christine NNP cord-269867-k10siwur 1 20 title title NN cord-269867-k10siwur 1 21 : : : cord-269867-k10siwur 1 22 The the DT cord-269867-k10siwur 1 23 major major JJ cord-269867-k10siwur 1 24 subunit subunit NN cord-269867-k10siwur 1 25 ClpG ClpG NNP cord-269867-k10siwur 1 26 of of IN cord-269867-k10siwur 1 27 Escherichia Escherichia NNP cord-269867-k10siwur 1 28 coli coli NNS cord-269867-k10siwur 1 29 CS31A CS31A NNP cord-269867-k10siwur 1 30 fibrillae fibrillae NNP cord-269867-k10siwur 1 31 as as IN cord-269867-k10siwur 1 32 an an DT cord-269867-k10siwur 1 33 expression expression NN cord-269867-k10siwur 1 34 vector vector NN cord-269867-k10siwur 1 35 for for IN cord-269867-k10siwur 1 36 different different JJ cord-269867-k10siwur 1 37 combinations combination NNS cord-269867-k10siwur 1 38 of of IN cord-269867-k10siwur 1 39 two two CD cord-269867-k10siwur 1 40 TGEV TGEV NNP cord-269867-k10siwur 1 41 coronavirus coronavirus NN cord-269867-k10siwur 1 42 epitopes epitope NNS cord-269867-k10siwur 1 43 date date NN cord-269867-k10siwur 1 44 : : : cord-269867-k10siwur 1 45 1996 1996 CD cord-269867-k10siwur 1 46 - - SYM cord-269867-k10siwur 1 47 12 12 CD cord-269867-k10siwur 1 48 - - SYM cord-269867-k10siwur 1 49 31 31 CD cord-269867-k10siwur 1 50 journal journal NN cord-269867-k10siwur 1 51 : : : cord-269867-k10siwur 2 1 Gene gene NN cord-269867-k10siwur 2 2 DOI DOI NNP cord-269867-k10siwur 2 3 : : : cord-269867-k10siwur 2 4 10.1016 10.1016 CD cord-269867-k10siwur 2 5 / / SYM cord-269867-k10siwur 2 6 s0378 s0378 NNP cord-269867-k10siwur 2 7 - - HYPH cord-269867-k10siwur 2 8 1119(96)00348 1119(96)00348 NNP cord-269867-k10siwur 2 9 - - HYPH cord-269867-k10siwur 2 10 4 4 CD cord-269867-k10siwur 2 11 sha sha NNP cord-269867-k10siwur 2 12 : : : cord-269867-k10siwur 3 1 b151730d74e21025def6ee80cd69132d3a4a2e21 b151730d74e21025def6ee80cd69132d3a4a2e21 NNP cord-269867-k10siwur 3 2 doc_id doc_id CD cord-269867-k10siwur 3 3 : : : cord-269867-k10siwur 3 4 269867 269867 CD cord-269867-k10siwur 4 1 cord_uid cord_uid NN cord-269867-k10siwur 4 2 : : : cord-269867-k10siwur 5 1 k10siwur k10siwur NNP cord-269867-k10siwur 5 2 Abstract Abstract NNP cord-269867-k10siwur 6 1 Previously previously RB cord-269867-k10siwur 6 2 , , , cord-269867-k10siwur 6 3 two two CD cord-269867-k10siwur 6 4 B b NN cord-269867-k10siwur 6 5 - - HYPH cord-269867-k10siwur 6 6 cell cell NN cord-269867-k10siwur 6 7 epitopes epitope NNS cord-269867-k10siwur 6 8 from from IN cord-269867-k10siwur 6 9 the the DT cord-269867-k10siwur 6 10 entero entero NNP cord-269867-k10siwur 6 11 - - HYPH cord-269867-k10siwur 6 12 pathogenic pathogenic JJ cord-269867-k10siwur 6 13 transmissible transmissible JJ cord-269867-k10siwur 6 14 gastroenteritis gastroenteritis NN cord-269867-k10siwur 6 15 virus virus NN cord-269867-k10siwur 6 16 ( ( -LRB- cord-269867-k10siwur 6 17 TGEV TGEV NNP cord-269867-k10siwur 6 18 ) ) -RRB- cord-269867-k10siwur 6 19 , , , cord-269867-k10siwur 6 20 namely namely RB cord-269867-k10siwur 6 21 the the DT cord-269867-k10siwur 6 22 C C NNP cord-269867-k10siwur 6 23 epitope epitope NN cord-269867-k10siwur 6 24 ( ( -LRB- cord-269867-k10siwur 6 25 TGEV TGEV NNP cord-269867-k10siwur 6 26 - - HYPH cord-269867-k10siwur 6 27 C C NNP cord-269867-k10siwur 6 28 ) ) -RRB- cord-269867-k10siwur 6 29 amino amino NN cord-269867-k10siwur 6 30 acids acid NNS cord-269867-k10siwur 6 31 ( ( -LRB- cord-269867-k10siwur 6 32 aa aa NNP cord-269867-k10siwur 6 33 ) ) -RRB- cord-269867-k10siwur 7 1 363–371 363–371 CD cord-269867-k10siwur 7 2 and and CC cord-269867-k10siwur 7 3 the the DT cord-269867-k10siwur 7 4 A a DT cord-269867-k10siwur 7 5 epitope epitope NN cord-269867-k10siwur 7 6 ( ( -LRB- cord-269867-k10siwur 7 7 TGEV TGEV NNP cord-269867-k10siwur 7 8 - - HYPH cord-269867-k10siwur 7 9 A a NN cord-269867-k10siwur 7 10 ) ) -RRB- cord-269867-k10siwur 8 1 aa aa NNP cord-269867-k10siwur 8 2 522–531 522–531 CD cord-269867-k10siwur 8 3 of of IN cord-269867-k10siwur 8 4 the the DT cord-269867-k10siwur 8 5 spike spike NN cord-269867-k10siwur 8 6 S s NN cord-269867-k10siwur 8 7 protein protein NN cord-269867-k10siwur 8 8 ( ( -LRB- cord-269867-k10siwur 8 9 TGEV TGEV NNP cord-269867-k10siwur 8 10 - - HYPH cord-269867-k10siwur 8 11 S S NNP cord-269867-k10siwur 8 12 ) ) -RRB- cord-269867-k10siwur 8 13 , , , cord-269867-k10siwur 8 14 have have VBP cord-269867-k10siwur 8 15 been be VBN cord-269867-k10siwur 8 16 separately separately RB cord-269867-k10siwur 8 17 expressed express VBN cord-269867-k10siwur 8 18 on on IN cord-269867-k10siwur 8 19 the the DT cord-269867-k10siwur 8 20 CS31A cs31a NN cord-269867-k10siwur 8 21 fibrillae fibrillae NNP cord-269867-k10siwur 8 22 at at IN cord-269867-k10siwur 8 23 the the DT cord-269867-k10siwur 8 24 surface surface NN cord-269867-k10siwur 8 25 of of IN cord-269867-k10siwur 8 26 Escherichia Escherichia NNP cord-269867-k10siwur 8 27 coli coli NNS cord-269867-k10siwur 8 28 following follow VBG cord-269867-k10siwur 8 29 insertion insertion NN cord-269867-k10siwur 8 30 into into IN cord-269867-k10siwur 8 31 a a DT cord-269867-k10siwur 8 32 same same JJ cord-269867-k10siwur 8 33 region region NN cord-269867-k10siwur 8 34 of of IN cord-269867-k10siwur 8 35 ClpG. ClpG. . cord-269867-k10siwur 9 1 However however RB cord-269867-k10siwur 9 2 , , , cord-269867-k10siwur 9 3 the the DT cord-269867-k10siwur 9 4 resulting result VBG cord-269867-k10siwur 9 5 chimeras chimera NNS cord-269867-k10siwur 9 6 induced induce VBD cord-269867-k10siwur 9 7 a a DT cord-269867-k10siwur 9 8 marginal marginal JJ cord-269867-k10siwur 9 9 TGEV TGEV NNP cord-269867-k10siwur 9 10 - - HYPH cord-269867-k10siwur 9 11 neutralizing neutralize VBG cord-269867-k10siwur 9 12 antibody antibody NN cord-269867-k10siwur 9 13 ( ( -LRB- cord-269867-k10siwur 9 14 Ab ab NN cord-269867-k10siwur 9 15 ) ) -RRB- cord-269867-k10siwur 9 16 response response NN cord-269867-k10siwur 9 17 in in IN cord-269867-k10siwur 9 18 mice mouse NNS cord-269867-k10siwur 9 19 . . . cord-269867-k10siwur 10 1 Here here RB cord-269867-k10siwur 10 2 , , , cord-269867-k10siwur 10 3 with with IN cord-269867-k10siwur 10 4 the the DT cord-269867-k10siwur 10 5 view view NN cord-269867-k10siwur 10 6 to to IN cord-269867-k10siwur 10 7 improving improve VBG cord-269867-k10siwur 10 8 this this DT cord-269867-k10siwur 10 9 response response NN cord-269867-k10siwur 10 10 , , , cord-269867-k10siwur 10 11 we -PRON- PRP cord-269867-k10siwur 10 12 introduced introduce VBD cord-269867-k10siwur 10 13 TGEV TGEV NNP cord-269867-k10siwur 10 14 - - HYPH cord-269867-k10siwur 10 15 C C NNP cord-269867-k10siwur 10 16 alone alone RB cord-269867-k10siwur 10 17 or or CC cord-269867-k10siwur 10 18 in in IN cord-269867-k10siwur 10 19 different different JJ cord-269867-k10siwur 10 20 tandem tandem NN cord-269867-k10siwur 10 21 association association NN cord-269867-k10siwur 10 22 with with IN cord-269867-k10siwur 10 23 TGEV TGEV NNP cord-269867-k10siwur 10 24 - - HYPH cord-269867-k10siwur 10 25 A A NNP cord-269867-k10siwur 10 26 ( ( -LRB- cord-269867-k10siwur 10 27 A::C a::c JJ cord-269867-k10siwur 10 28 or or CC cord-269867-k10siwur 10 29 C::A C::A NNP cord-269867-k10siwur 10 30 ) ) -RRB- cord-269867-k10siwur 10 31 in in IN cord-269867-k10siwur 10 32 twelve twelve CD cord-269867-k10siwur 10 33 putatively putatively RB cord-269867-k10siwur 10 34 exposed expose VBN cord-269867-k10siwur 10 35 regions region NNS cord-269867-k10siwur 10 36 of of IN cord-269867-k10siwur 10 37 ClpG. ClpG. . cord-269867-k10siwur 11 1 Among among IN cord-269867-k10siwur 11 2 the the DT cord-269867-k10siwur 11 3 28 28 CD cord-269867-k10siwur 11 4 resulting result VBG cord-269867-k10siwur 11 5 engineered engineer VBN cord-269867-k10siwur 11 6 proteins protein NNS cord-269867-k10siwur 11 7 only only RB cord-269867-k10siwur 11 8 15 15 CD cord-269867-k10siwur 11 9 , , , cord-269867-k10siwur 11 10 carrying carry VBG cord-269867-k10siwur 11 11 up up RP cord-269867-k10siwur 11 12 to to TO cord-269867-k10siwur 11 13 51 51 CD cord-269867-k10siwur 11 14 extra extra JJ cord-269867-k10siwur 11 15 aa aa NN cord-269867-k10siwur 11 16 , , , cord-269867-k10siwur 11 17 had have VBD cord-269867-k10siwur 11 18 not not RB cord-269867-k10siwur 11 19 essentially essentially RB cord-269867-k10siwur 11 20 disturbed disturb VBN cord-269867-k10siwur 11 21 the the DT cord-269867-k10siwur 11 22 correct correct JJ cord-269867-k10siwur 11 23 CS31A cs31a NN cord-269867-k10siwur 11 24 fibrillae fibrillae NNP cord-269867-k10siwur 11 25 formation formation NN cord-269867-k10siwur 11 26 process process NN cord-269867-k10siwur 11 27 . . . cord-269867-k10siwur 12 1 Six six CD cord-269867-k10siwur 12 2 partially partially RB cord-269867-k10siwur 12 3 permissive permissive JJ cord-269867-k10siwur 12 4 sites site NNS cord-269867-k10siwur 12 5 accepting accept VBG cord-269867-k10siwur 12 6 only only RB cord-269867-k10siwur 12 7 TGEV TGEV NNP cord-269867-k10siwur 12 8 - - HYPH cord-269867-k10siwur 12 9 C C NNP cord-269867-k10siwur 12 10 and and CC cord-269867-k10siwur 12 11 three three CD cord-269867-k10siwur 12 12 highly highly RB cord-269867-k10siwur 12 13 permissive permissive JJ cord-269867-k10siwur 12 14 sites site NNS cord-269867-k10siwur 12 15 tolerating tolerate VBG cord-269867-k10siwur 12 16 A::C a::c JJ cord-269867-k10siwur 12 17 or or CC cord-269867-k10siwur 12 18 C::A c::a JJ cord-269867-k10siwur 12 19 tandem tandem NN cord-269867-k10siwur 12 20 peptide peptide NN cord-269867-k10siwur 12 21 , , , cord-269867-k10siwur 12 22 were be VBD cord-269867-k10siwur 12 23 identified identify VBN cord-269867-k10siwur 12 24 throughout throughout IN cord-269867-k10siwur 12 25 ClpG. ClpG. . cord-269867-k10siwur 13 1 Intact intact JJ cord-269867-k10siwur 13 2 bacteria bacteria NNS cord-269867-k10siwur 13 3 or or CC cord-269867-k10siwur 13 4 extracted extract VBN cord-269867-k10siwur 13 5 CS31A cs31a NN cord-269867-k10siwur 13 6 hybrid hybrid JJ cord-269867-k10siwur 13 7 fibrillae fibrillae NNP cord-269867-k10siwur 13 8 expressing express VBG cord-269867-k10siwur 13 9 TGEV TGEV NNP cord-269867-k10siwur 13 10 epitopes epitope NNS cord-269867-k10siwur 13 11 at at IN cord-269867-k10siwur 13 12 any any DT cord-269867-k10siwur 13 13 of of IN cord-269867-k10siwur 13 14 the the DT cord-269867-k10siwur 13 15 permissive permissive JJ cord-269867-k10siwur 13 16 sites site NNS cord-269867-k10siwur 13 17 , , , cord-269867-k10siwur 13 18 were be VBD cord-269867-k10siwur 13 19 recognized recognize VBN cord-269867-k10siwur 13 20 by by IN cord-269867-k10siwur 13 21 Ab Ab NNP cord-269867-k10siwur 13 22 directed direct VBN cord-269867-k10siwur 13 23 against against IN cord-269867-k10siwur 13 24 the the DT cord-269867-k10siwur 13 25 foreign foreign JJ cord-269867-k10siwur 13 26 parent parent NN cord-269867-k10siwur 13 27 protein protein NN cord-269867-k10siwur 13 28 , , , cord-269867-k10siwur 13 29 providing provide VBG cord-269867-k10siwur 13 30 a a DT cord-269867-k10siwur 13 31 direct direct JJ cord-269867-k10siwur 13 32 argument argument NN cord-269867-k10siwur 13 33 for for IN cord-269867-k10siwur 13 34 exposure exposure NN cord-269867-k10siwur 13 35 of of IN cord-269867-k10siwur 13 36 the the DT cord-269867-k10siwur 13 37 corresponding correspond VBG cord-269867-k10siwur 13 38 ClpG ClpG NNP cord-269867-k10siwur 13 39 region region NN cord-269867-k10siwur 13 40 at at IN cord-269867-k10siwur 13 41 the the DT cord-269867-k10siwur 13 42 cell cell NN cord-269867-k10siwur 13 43 surface surface NN cord-269867-k10siwur 13 44 and and CC cord-269867-k10siwur 13 45 for for IN cord-269867-k10siwur 13 46 antigenicity antigenicity NN cord-269867-k10siwur 13 47 of of IN cord-269867-k10siwur 13 48 the the DT cord-269867-k10siwur 13 49 epitopes epitope NNS cord-269867-k10siwur 13 50 in in IN cord-269867-k10siwur 13 51 the the DT cord-269867-k10siwur 13 52 polymeric polymeric JJ cord-269867-k10siwur 13 53 CS31A cs31a NN cord-269867-k10siwur 13 54 fibrillae fibrillae NNP cord-269867-k10siwur 13 55 context context NN cord-269867-k10siwur 13 56 . . . cord-269867-k10siwur 14 1 The the DT cord-269867-k10siwur 14 2 potential potential NN cord-269867-k10siwur 14 3 of of IN cord-269867-k10siwur 14 4 CS31A CS31A NNP cord-269867-k10siwur 14 5 fibrillae fibrillae NNP cord-269867-k10siwur 14 6 as as IN cord-269867-k10siwur 14 7 carriers carrier NNS cord-269867-k10siwur 14 8 of of IN cord-269867-k10siwur 14 9 the the DT cord-269867-k10siwur 14 10 TGEV TGEV NNP cord-269867-k10siwur 14 11 peptides peptide NNS cord-269867-k10siwur 14 12 indicates indicate VBZ cord-269867-k10siwur 14 13 that that IN cord-269867-k10siwur 14 14 there there EX cord-269867-k10siwur 14 15 may may MD cord-269867-k10siwur 14 16 be be VB cord-269867-k10siwur 14 17 three three CD cord-269867-k10siwur 14 18 positions position NNS cord-269867-k10siwur 14 19 ( ( -LRB- cord-269867-k10siwur 14 20 N n CD cord-269867-k10siwur 14 21 terminus terminus NN cord-269867-k10siwur 14 22 , , , cord-269867-k10siwur 14 23 aa aa NNP cord-269867-k10siwur 14 24 202–204 202–204 CD cord-269867-k10siwur 14 25 and and CC cord-269867-k10siwur 14 26 202–218 202–218 CD cord-269867-k10siwur 14 27 ) ) -RRB- cord-269867-k10siwur 14 28 in in IN cord-269867-k10siwur 14 29 ClpG ClpG NNP cord-269867-k10siwur 14 30 which which WDT cord-269867-k10siwur 14 31 may may MD cord-269867-k10siwur 14 32 turn turn VB cord-269867-k10siwur 14 33 out out RP cord-269867-k10siwur 14 34 to to TO cord-269867-k10siwur 14 35 be be VB cord-269867-k10siwur 14 36 important important JJ cord-269867-k10siwur 14 37 fusion fusion NN cord-269867-k10siwur 14 38 sites site NNS cord-269867-k10siwur 14 39 and and CC cord-269867-k10siwur 14 40 therefore therefore RB cord-269867-k10siwur 14 41 be be VB cord-269867-k10siwur 14 42 relevant relevant JJ cord-269867-k10siwur 14 43 for for IN cord-269867-k10siwur 14 44 the the DT cord-269867-k10siwur 14 45 eventual eventual JJ cord-269867-k10siwur 14 46 design design NN cord-269867-k10siwur 14 47 of of IN cord-269867-k10siwur 14 48 TGEV TGEV NNP cord-269867-k10siwur 14 49 vaccines vaccine NNS cord-269867-k10siwur 14 50 . . . cord-269867-k10siwur 15 1 Unexpectedly unexpectedly RB cord-269867-k10siwur 15 2 , , , cord-269867-k10siwur 15 3 TGEV TGEV NNP cord-269867-k10siwur 15 4 - - HYPH cord-269867-k10siwur 15 5 A A NNP cord-269867-k10siwur 15 6 , , , cord-269867-k10siwur 15 7 whatever whatever WDT cord-269867-k10siwur 15 8 its -PRON- PRP$ cord-269867-k10siwur 15 9 position position NN cord-269867-k10siwur 15 10 in in IN cord-269867-k10siwur 15 11 ClpG ClpG NNP cord-269867-k10siwur 15 12 , , , cord-269867-k10siwur 15 13 mediated mediate VBD cord-269867-k10siwur 15 14 the the DT cord-269867-k10siwur 15 15 partial partial JJ cord-269867-k10siwur 15 16 proteolytic proteolytic JJ cord-269867-k10siwur 15 17 degradation degradation NN cord-269867-k10siwur 15 18 of of IN cord-269867-k10siwur 15 19 the the DT cord-269867-k10siwur 15 20 hybrid hybrid JJ cord-269867-k10siwur 15 21 proteins protein NNS cord-269867-k10siwur 15 22 , , , cord-269867-k10siwur 15 23 suggesting suggest VBG cord-269867-k10siwur 15 24 that that IN cord-269867-k10siwur 15 25 it -PRON- PRP cord-269867-k10siwur 15 26 functions function VBZ cord-269867-k10siwur 15 27 as as IN cord-269867-k10siwur 15 28 a a DT cord-269867-k10siwur 15 29 substrate substrate NN cord-269867-k10siwur 15 30 for for IN cord-269867-k10siwur 15 31 a a DT cord-269867-k10siwur 15 32 cellular cellular JJ cord-269867-k10siwur 15 33 protease protease NN cord-269867-k10siwur 15 34 , , , cord-269867-k10siwur 15 35 and and CC cord-269867-k10siwur 15 36 thereby thereby RB cord-269867-k10siwur 15 37 that that IN cord-269867-k10siwur 15 38 its -PRON- PRP$ cord-269867-k10siwur 15 39 suitability suitability NN cord-269867-k10siwur 15 40 as as IN cord-269867-k10siwur 15 41 a a DT cord-269867-k10siwur 15 42 vaccine vaccine NN cord-269867-k10siwur 15 43 antigen antigen NN cord-269867-k10siwur 15 44 candidate candidate NN cord-269867-k10siwur 15 45 is be VBZ cord-269867-k10siwur 15 46 doubtful doubtful JJ cord-269867-k10siwur 15 47 . . . cord-269867-k10siwur 16 1 Epitope epitope NN cord-269867-k10siwur 16 2 - - HYPH cord-269867-k10siwur 16 3 based base VBN cord-269867-k10siwur 16 4 recombinant recombinant JJ cord-269867-k10siwur 16 5 vaccine vaccine NN cord-269867-k10siwur 16 6 technologies technology NNS cord-269867-k10siwur 16 7 offer offer VBP cord-269867-k10siwur 16 8 the the DT cord-269867-k10siwur 16 9 potential potential NN cord-269867-k10siwur 16 10 for for IN cord-269867-k10siwur 16 11 oral oral JJ cord-269867-k10siwur 16 12 or or CC cord-269867-k10siwur 16 13 mucosal mucosal JJ cord-269867-k10siwur 16 14 delivery delivery NN cord-269867-k10siwur 16 15 , , , cord-269867-k10siwur 16 16 especially especially RB cord-269867-k10siwur 16 17 when when WRB cord-269867-k10siwur 16 18 the the DT cord-269867-k10siwur 16 19 relevant relevant JJ cord-269867-k10siwur 16 20 epitope epitope NN cord-269867-k10siwur 16 21 genetically genetically RB cord-269867-k10siwur 16 22 fused fuse VBN cord-269867-k10siwur 16 23 to to IN cord-269867-k10siwur 16 24 a a DT cord-269867-k10siwur 16 25 carrier carrier NN cord-269867-k10siwur 16 26 protein protein NN cord-269867-k10siwur 16 27 , , , cord-269867-k10siwur 16 28 is be VBZ cord-269867-k10siwur 16 29 displayed display VBN cord-269867-k10siwur 16 30 as as IN cord-269867-k10siwur 16 31 a a DT cord-269867-k10siwur 16 32 heterologous heterologous JJ cord-269867-k10siwur 16 33 peptide peptide NN cord-269867-k10siwur 16 34 on on IN cord-269867-k10siwur 16 35 the the DT cord-269867-k10siwur 16 36 surface surface NN cord-269867-k10siwur 16 37 of of IN cord-269867-k10siwur 16 38 a a DT cord-269867-k10siwur 16 39 bacterial bacterial JJ cord-269867-k10siwur 16 40 strain strain NN cord-269867-k10siwur 16 41 ( ( -LRB- cord-269867-k10siwur 16 42 Rabinovich Rabinovich NNP cord-269867-k10siwur 16 43 et et NNP cord-269867-k10siwur 16 44 al al NNP cord-269867-k10siwur 16 45 . . NNP cord-269867-k10siwur 16 46 , , , cord-269867-k10siwur 16 47 1994 1994 CD cord-269867-k10siwur 16 48 ) ) -RRB- cord-269867-k10siwur 16 49 . . . cord-269867-k10siwur 17 1 However however RB cord-269867-k10siwur 17 2 , , , cord-269867-k10siwur 17 3 coupling couple VBG cord-269867-k10siwur 17 4 foreign foreign JJ cord-269867-k10siwur 17 5 peptides peptide NNS cord-269867-k10siwur 17 6 to to IN cord-269867-k10siwur 17 7 carriers carrier NNS cord-269867-k10siwur 17 8 can can MD cord-269867-k10siwur 17 9 result result VB cord-269867-k10siwur 17 10 in in IN cord-269867-k10siwur 17 11 a a DT cord-269867-k10siwur 17 12 poor poor JJ cord-269867-k10siwur 17 13 immunogenicity immunogenicity NN cord-269867-k10siwur 17 14 of of IN cord-269867-k10siwur 17 15 the the DT cord-269867-k10siwur 17 16 chimeric chimeric JJ cord-269867-k10siwur 17 17 antigens antigen NNS cord-269867-k10siwur 17 18 due due IN cord-269867-k10siwur 17 19 to to IN cord-269867-k10siwur 17 20 the the DT cord-269867-k10siwur 17 21 local local JJ cord-269867-k10siwur 17 22 conformational conformational JJ cord-269867-k10siwur 17 23 restrictions restriction NNS cord-269867-k10siwur 17 24 imposed impose VBN cord-269867-k10siwur 17 25 on on IN cord-269867-k10siwur 17 26 epitopes epitope NNS cord-269867-k10siwur 17 27 by by IN cord-269867-k10siwur 17 28 the the DT cord-269867-k10siwur 17 29 embedding embed VBG cord-269867-k10siwur 17 30 structure structure NN cord-269867-k10siwur 17 31 ( ( -LRB- cord-269867-k10siwur 17 32 Benito Benito NNP cord-269867-k10siwur 17 33 et et NNP cord-269867-k10siwur 17 34 al al NNP cord-269867-k10siwur 17 35 . . NNP cord-269867-k10siwur 17 36 , , , cord-269867-k10siwur 17 37 1995 1995 CD cord-269867-k10siwur 17 38 ) ) -RRB- cord-269867-k10siwur 17 39 . . . cord-269867-k10siwur 18 1 Therefore therefore RB cord-269867-k10siwur 18 2 , , , cord-269867-k10siwur 18 3 approaches approach VBZ cord-269867-k10siwur 18 4 to to IN cord-269867-k10siwur 18 5 enhancing enhance VBG cord-269867-k10siwur 18 6 immunogenicity immunogenicity NN cord-269867-k10siwur 18 7 are be VBP cord-269867-k10siwur 18 8 critical critical JJ cord-269867-k10siwur 18 9 . . . cord-269867-k10siwur 19 1 Since since IN cord-269867-k10siwur 19 2 the the DT cord-269867-k10siwur 19 3 conformation conformation NN cord-269867-k10siwur 19 4 of of IN cord-269867-k10siwur 19 5 a a DT cord-269867-k10siwur 19 6 foreign foreign JJ cord-269867-k10siwur 19 7 sequence sequence NN cord-269867-k10siwur 19 8 within within IN cord-269867-k10siwur 19 9 a a DT cord-269867-k10siwur 19 10 carrier carrier NN cord-269867-k10siwur 19 11 varies vary VBZ cord-269867-k10siwur 19 12 widely widely RB cord-269867-k10siwur 19 13 depending depend VBG cord-269867-k10siwur 19 14 on on IN cord-269867-k10siwur 19 15 the the DT cord-269867-k10siwur 19 16 flanking flank VBG cord-269867-k10siwur 19 17 sequences sequence NNS cord-269867-k10siwur 19 18 ( ( -LRB- cord-269867-k10siwur 19 19 Tishminetzky Tishminetzky NNP cord-269867-k10siwur 19 20 et et FW cord-269867-k10siwur 19 21 al al NNP cord-269867-k10siwur 19 22 . . NNP cord-269867-k10siwur 19 23 , , , cord-269867-k10siwur 19 24 1994 1994 CD cord-269867-k10siwur 19 25 ) ) -RRB- cord-269867-k10siwur 19 26 , , , cord-269867-k10siwur 19 27 the the DT cord-269867-k10siwur 19 28 insertion insertion NN cord-269867-k10siwur 19 29 of of IN cord-269867-k10siwur 19 30 an an DT cord-269867-k10siwur 19 31 antigen antigen NN cord-269867-k10siwur 19 32 into into IN cord-269867-k10siwur 19 33 different different JJ cord-269867-k10siwur 19 34 exposed expose VBN cord-269867-k10siwur 19 35 regions region NNS cord-269867-k10siwur 19 36 of of IN cord-269867-k10siwur 19 37 a a DT cord-269867-k10siwur 19 38 delivery delivery NN cord-269867-k10siwur 19 39 protein protein NN cord-269867-k10siwur 19 40 , , , cord-269867-k10siwur 19 41 thus thus RB cord-269867-k10siwur 19 42 changing change VBG cord-269867-k10siwur 19 43 antigen antigen NN cord-269867-k10siwur 19 44 conformation conformation NN cord-269867-k10siwur 19 45 , , , cord-269867-k10siwur 19 46 could could MD cord-269867-k10siwur 19 47 permit permit VB cord-269867-k10siwur 19 48 a a DT cord-269867-k10siwur 19 49 strategy strategy NN cord-269867-k10siwur 19 50 for for IN cord-269867-k10siwur 19 51 finding find VBG cord-269867-k10siwur 19 52 appropriate appropriate JJ cord-269867-k10siwur 19 53 environments environment NNS cord-269867-k10siwur 19 54 for for IN cord-269867-k10siwur 19 55 peptide peptide JJ cord-269867-k10siwur 19 56 presentation presentation NN cord-269867-k10siwur 19 57 among among IN cord-269867-k10siwur 19 58 the the DT cord-269867-k10siwur 19 59 complex complex JJ cord-269867-k10siwur 19 60 assortment assortment NN cord-269867-k10siwur 19 61 of of IN cord-269867-k10siwur 19 62 molecular molecular JJ cord-269867-k10siwur 19 63 contexts context NNS cord-269867-k10siwur 19 64 offered offer VBN cord-269867-k10siwur 19 65 by by IN cord-269867-k10siwur 19 66 the the DT cord-269867-k10siwur 19 67 carrier carrier NN cord-269867-k10siwur 19 68 . . . cord-269867-k10siwur 20 1 A a DT cord-269867-k10siwur 20 2 second second JJ cord-269867-k10siwur 20 3 strategy strategy NN cord-269867-k10siwur 20 4 to to IN cord-269867-k10siwur 20 5 improving improve VBG cord-269867-k10siwur 20 6 immunogenicity immunogenicity NN cord-269867-k10siwur 20 7 is be VBZ cord-269867-k10siwur 20 8 the the DT cord-269867-k10siwur 20 9 fusion fusion NN cord-269867-k10siwur 20 10 of of IN cord-269867-k10siwur 20 11 The the DT cord-269867-k10siwur 20 12 one one CD cord-269867-k10siwur 20 13 - - HYPH cord-269867-k10siwur 20 14 letter letter NN cord-269867-k10siwur 20 15 code code NN cord-269867-k10siwur 20 16 for for IN cord-269867-k10siwur 20 17 aa aa NNP cord-269867-k10siwur 20 18 designation designation NN cord-269867-k10siwur 20 19 is be VBZ cord-269867-k10siwur 20 20 used use VBN cord-269867-k10siwur 20 21 . . . cord-269867-k10siwur 21 1 Numbered number VBN cord-269867-k10siwur 21 2 vertical vertical JJ cord-269867-k10siwur 21 3 arrows arrow NNS cord-269867-k10siwur 21 4 above above IN cord-269867-k10siwur 21 5 the the DT cord-269867-k10siwur 21 6 nt nt NNP cord-269867-k10siwur 21 7 sequence sequence NN cord-269867-k10siwur 21 8 indicate indicate VBP cord-269867-k10siwur 21 9 the the DT cord-269867-k10siwur 21 10 number number NN cord-269867-k10siwur 21 11 and and CC cord-269867-k10siwur 21 12 position position NN cord-269867-k10siwur 21 13 of of IN cord-269867-k10siwur 21 14 the the DT cord-269867-k10siwur 21 15 twelve twelve CD cord-269867-k10siwur 21 16 selected select VBN cord-269867-k10siwur 21 17 insertion insertion NN cord-269867-k10siwur 21 18 sites site NNS cord-269867-k10siwur 21 19 . . . cord-269867-k10siwur 22 1 Asterisks asterisk NNS cord-269867-k10siwur 22 2 denote denote VBP cord-269867-k10siwur 22 3 the the DT cord-269867-k10siwur 22 4 nt nt RB cord-269867-k10siwur 22 5 modified modify VBN cord-269867-k10siwur 22 6 by by IN cord-269867-k10siwur 22 7 site site NN cord-269867-k10siwur 22 8 - - HYPH cord-269867-k10siwur 22 9 directed direct VBN cord-269867-k10siwur 22 10 mutagenesis mutagenesis NN cord-269867-k10siwur 22 11 and and CC cord-269867-k10siwur 22 12 the the DT cord-269867-k10siwur 22 13 corresponding correspond VBG cord-269867-k10siwur 22 14 mutated mutate VBN cord-269867-k10siwur 22 15 aa aa NN cord-269867-k10siwur 22 16 residue residue NN cord-269867-k10siwur 22 17 . . . cord-269867-k10siwur 23 1 The the DT cord-269867-k10siwur 23 2 resulting result VBG cord-269867-k10siwur 23 3 engineered engineer VBN cord-269867-k10siwur 23 4 restriction restriction NN cord-269867-k10siwur 23 5 sites site NNS cord-269867-k10siwur 23 6 SpeI SpeI NNP cord-269867-k10siwur 23 7 and and CC cord-269867-k10siwur 23 8 BgllI BgllI NNP cord-269867-k10siwur 23 9 in in IN cord-269867-k10siwur 23 10 plasmids plasmid NNS cord-269867-k10siwur 23 11 pPSX6S ppsx6s POS cord-269867-k10siwur 23 12 and and CC cord-269867-k10siwur 23 13 pPSX10S pPSX10S . cord-269867-k10siwur 24 1 ( ( -LRB- cord-269867-k10siwur 24 2 Bousquet Bousquet NNP cord-269867-k10siwur 24 3 et et NNP cord-269867-k10siwur 24 4 al al NNP cord-269867-k10siwur 24 5 . . NNP cord-269867-k10siwur 24 6 , , , cord-269867-k10siwur 24 7 1994 1994 CD cord-269867-k10siwur 24 8 ) ) -RRB- cord-269867-k10siwur 24 9 , , , cord-269867-k10siwur 24 10 coding code VBG cord-269867-k10siwur 24 11 for for IN cord-269867-k10siwur 24 12 mutants mutant NNS cord-269867-k10siwur 24 13 ClpG202 ClpG202 NNP cord-269867-k10siwur 24 14 and and CC cord-269867-k10siwur 24 15 CIpG420 cipg420 NN cord-269867-k10siwur 24 16 , , , cord-269867-k10siwur 24 17 are be VBP cord-269867-k10siwur 24 18 mentioned mention VBN cord-269867-k10siwur 24 19 . . . cord-269867-k10siwur 25 1 ( ( -LRB- cord-269867-k10siwur 25 2 a a LS cord-269867-k10siwur 25 3 - - HYPH cord-269867-k10siwur 25 4 e e NN cord-269867-k10siwur 25 5 ) ) -RRB- cord-269867-k10siwur 25 6 in in IN cord-269867-k10siwur 25 7 panel panel NN cord-269867-k10siwur 25 8 C C NNP cord-269867-k10siwur 25 9 represent represent VBP cord-269867-k10siwur 25 10 ( ( -LRB- cord-269867-k10siwur 25 11 a a LS cord-269867-k10siwur 25 12 ) ) -RRB- cord-269867-k10siwur 25 13 predicted predict VBN cord-269867-k10siwur 25 14 secondary secondary JJ cord-269867-k10siwur 25 15 structured structure VBN cord-269867-k10siwur 25 16 , , , cord-269867-k10siwur 25 17 ( ( -LRB- cord-269867-k10siwur 25 18 b b LS cord-269867-k10siwur 25 19 ) ) -RRB- cord-269867-k10siwur 25 20 hydrophilic hydrophilic JJ cord-269867-k10siwur 25 21 ( ( -LRB- cord-269867-k10siwur 25 22 hatched hatch VBN cord-269867-k10siwur 25 23 box box NN cord-269867-k10siwur 25 24 ) ) -RRB- cord-269867-k10siwur 25 25 , , , cord-269867-k10siwur 25 26 ( ( -LRB- cord-269867-k10siwur 25 27 c c NN cord-269867-k10siwur 25 28 ) ) -RRB- cord-269867-k10siwur 25 29 variable variable JJ cord-269867-k10siwur 25 30 ( ( -LRB- cord-269867-k10siwur 25 31 black black JJ cord-269867-k10siwur 25 32 boxes box NNS cord-269867-k10siwur 25 33 ) ) -RRB- cord-269867-k10siwur 25 34 or or CC cord-269867-k10siwur 25 35 conserved conserve VBN cord-269867-k10siwur 25 36 ( ( -LRB- cord-269867-k10siwur 25 37 open open JJ cord-269867-k10siwur 25 38 boxes box NNS cord-269867-k10siwur 25 39 ) ) -RRB- cord-269867-k10siwur 26 1 ( ( -LRB- cord-269867-k10siwur 26 2 Girardeau Girardeau NNP cord-269867-k10siwur 26 3 et et NNP cord-269867-k10siwur 26 4 al al NNP cord-269867-k10siwur 26 5 . . . cord-269867-k10siwur 27 1 , , , cord-269867-k10siwur 27 2 1991 1991 CD cord-269867-k10siwur 27 3 ; ; : cord-269867-k10siwur 27 4 Mrchin Mrchin NNP cord-269867-k10siwur 27 5 et et NNP cord-269867-k10siwur 27 6 al al NNP cord-269867-k10siwur 27 7 . . NNP cord-269867-k10siwur 27 8 , , , cord-269867-k10siwur 27 9 1995 1995 CD cord-269867-k10siwur 27 10 ) ) -RRB- cord-269867-k10siwur 28 1 ( ( -LRB- cord-269867-k10siwur 28 2 Stanssens Stanssens NNP cord-269867-k10siwur 28 3 et et NNP cord-269867-k10siwur 28 4 al al NNP cord-269867-k10siwur 28 5 . . NNP cord-269867-k10siwur 28 6 , , , cord-269867-k10siwur 28 7 1989 1989 CD cord-269867-k10siwur 28 8 ) ) -RRB- cord-269867-k10siwur 28 9 , , , cord-269867-k10siwur 28 10 or or CC cord-269867-k10siwur 28 11 performed perform VBN cord-269867-k10siwur 28 12 on on IN cord-269867-k10siwur 28 13 double double RB cord-269867-k10siwur 28 14 - - HYPH cord-269867-k10siwur 28 15 stranded stranded JJ cord-269867-k10siwur 28 16 DNA dna NN cord-269867-k10siwur 28 17 using use VBG cord-269867-k10siwur 28 18 an an DT cord-269867-k10siwur 28 19 adapted adapt VBN cord-269867-k10siwur 28 20 protocol protocol NN cord-269867-k10siwur 28 21 from from IN cord-269867-k10siwur 28 22 the the DT cord-269867-k10siwur 28 23 methods method NNS cord-269867-k10siwur 28 24 of of IN cord-269867-k10siwur 28 25 Jung Jung NNP cord-269867-k10siwur 28 26 et et NNP cord-269867-k10siwur 28 27 al al NNP cord-269867-k10siwur 28 28 . . . cord-269867-k10siwur 29 1 ( ( -LRB- cord-269867-k10siwur 29 2 1992 1992 CD cord-269867-k10siwur 29 3 ) ) -RRB- cord-269867-k10siwur 29 4 and and CC cord-269867-k10siwur 29 5 Deng Deng NNP cord-269867-k10siwur 29 6 and and CC cord-269867-k10siwur 29 7 Nickoloff Nickoloff NNP cord-269867-k10siwur 29 8 ( ( -LRB- cord-269867-k10siwur 29 9 1992 1992 CD cord-269867-k10siwur 29 10 ) ) -RRB- cord-269867-k10siwur 29 11 . . . cord-269867-k10siwur 30 1 Mutations mutation NNS cord-269867-k10siwur 30 2 were be VBD cord-269867-k10siwur 30 3 verified verify VBN cord-269867-k10siwur 30 4 by by IN cord-269867-k10siwur 30 5 DNA dna NN cord-269867-k10siwur 30 6 sequencing sequencing NN cord-269867-k10siwur 30 7 ( ( -LRB- cord-269867-k10siwur 30 8 Sanger Sanger NNP cord-269867-k10siwur 30 9 et et NNP cord-269867-k10siwur 30 10 al al NNP cord-269867-k10siwur 30 11 . . NNP cord-269867-k10siwur 30 12 , , , cord-269867-k10siwur 30 13 1977 1977 CD cord-269867-k10siwur 30 14 ) ) -RRB- cord-269867-k10siwur 30 15 . . . cord-269867-k10siwur 31 1 The the DT cord-269867-k10siwur 31 2 oligos oligo NNS cord-269867-k10siwur 31 3 used use VBN cord-269867-k10siwur 31 4 in in IN cord-269867-k10siwur 31 5 this this DT cord-269867-k10siwur 31 6 study study NN cord-269867-k10siwur 31 7 were be VBD cord-269867-k10siwur 31 8 synthesized synthesize VBN cord-269867-k10siwur 31 9 and and CC cord-269867-k10siwur 31 10 , , , cord-269867-k10siwur 31 11 when when WRB cord-269867-k10siwur 31 12 necessary necessary JJ cord-269867-k10siwur 31 13 , , , cord-269867-k10siwur 31 14 PAGE page NN cord-269867-k10siwur 31 15 - - HYPH cord-269867-k10siwur 31 16 purified purify VBN cord-269867-k10siwur 31 17 and and CC cord-269867-k10siwur 31 18 5'-phosphoryled 5'-phosphoryled CD cord-269867-k10siwur 32 1 ( ( -LRB- cord-269867-k10siwur 32 2 Eurogentec Eurogentec NNP cord-269867-k10siwur 32 3 , , , cord-269867-k10siwur 32 4 Belgium Belgium NNP cord-269867-k10siwur 32 5 ) ) -RRB- cord-269867-k10siwur 32 6 ; ; : cord-269867-k10siwur 32 7 in in IN cord-269867-k10siwur 32 8 the the DT cord-269867-k10siwur 32 9 oligos oligo NNS cord-269867-k10siwur 32 10 shown show VBN cord-269867-k10siwur 32 11 below below RB cord-269867-k10siwur 32 12 , , , cord-269867-k10siwur 32 13 names name NNS cord-269867-k10siwur 32 14 are be VBP cord-269867-k10siwur 32 15 indicated indicate VBN cord-269867-k10siwur 32 16 , , , cord-269867-k10siwur 32 17 numbers number NNS cord-269867-k10siwur 32 18 in in IN cord-269867-k10siwur 32 19 parentheses parenthesis NNS cord-269867-k10siwur 32 20 refer refer VBP cord-269867-k10siwur 32 21 to to IN cord-269867-k10siwur 32 22 nt nt RB cord-269867-k10siwur 32 23 position position VB cord-269867-k10siwur 32 24 in in IN cord-269867-k10siwur 32 25 the the DT cord-269867-k10siwur 32 26 coding coding NN cord-269867-k10siwur 32 27 strand strand NN cord-269867-k10siwur 32 28 of of IN cord-269867-k10siwur 32 29 the the DT cord-269867-k10siwur 32 30 clpG clpG NNS cord-269867-k10siwur 32 31 gene gene NN cord-269867-k10siwur 32 32 ( ( -LRB- cord-269867-k10siwur 32 33 see see VB cord-269867-k10siwur 32 34 C C NNP cord-269867-k10siwur 32 35 ) ) -RRB- cord-269867-k10siwur 32 36 , , , cord-269867-k10siwur 32 37 and and CC cord-269867-k10siwur 32 38 bold bold JJ cord-269867-k10siwur 32 39 characters character NNS cord-269867-k10siwur 32 40 represent represent VBP cord-269867-k10siwur 32 41 mutations mutation NNS cord-269867-k10siwur 32 42 resulting result VBG cord-269867-k10siwur 32 43 of of IN cord-269867-k10siwur 32 44 the the DT cord-269867-k10siwur 32 45 SpeI SpeI NNP cord-269867-k10siwur 32 46 site site NN cord-269867-k10siwur 32 47 introduction introduction NN cord-269867-k10siwur 32 48 : : : cord-269867-k10siwur 32 49 VN1 VN1 NNP cord-269867-k10siwur 32 50 , , , cord-269867-k10siwur 32 51 ( ( -LRB- cord-269867-k10siwur 32 52 559)CTTCAAGCAGTAACTAGTAACCCTAACGCG(582 559)cttcaagcagtaactagtaaccctaacgcg(582 CD cord-269867-k10siwur 32 53 ) ) -RRB- cord-269867-k10siwur 32 54 ; ; : cord-269867-k10siwur 32 55 NP2 NP2 NNP cord-269867-k10siwur 32 56 , , , cord-269867-k10siwur 32 57 ( ( -LRB- cord-269867-k10siwur 32 58 562)CAAGCAGTAAACACTAGTCCTAACGCGGGC(585 562)caagcagtaaacactagtcctaacgcgggc(585 CD cord-269867-k10siwur 32 59 ) ) -RRB- cord-269867-k10siwur 32 60 ; ; : cord-269867-k10siwur 32 61 PN3 pn3 NN cord-269867-k10siwur 32 62 , , , cord-269867-k10siwur 32 63 ( ( -LRB- cord-269867-k10siwur 32 64 565)GCAGTAAACCCTACTAGTAACGCGGGCAAT(588 565)gcagtaaaccctactagtaacgcgggcaat(588 CD cord-269867-k10siwur 32 65 ) ) -RRB- cord-269867-k10siwur 32 66 ; ; : cord-269867-k10siwur 32 67 NA4 NA4 NNP cord-269867-k10siwur 32 68 , , , cord-269867-k10siwur 32 69 tandem tandem NN cord-269867-k10siwur 32 70 peptides peptide NNS cord-269867-k10siwur 32 71 to to IN cord-269867-k10siwur 32 72 immunogenic immunogenic JJ cord-269867-k10siwur 32 73 carrier carrier NN cord-269867-k10siwur 32 74 proteins protein NNS cord-269867-k10siwur 33 1 ( ( -LRB- cord-269867-k10siwur 33 2 Broekhuijsen Broekhuijsen NNP cord-269867-k10siwur 33 3 et et NNP cord-269867-k10siwur 33 4 al al NNP cord-269867-k10siwur 33 5 . . . cord-269867-k10siwur 34 1 , , , cord-269867-k10siwur 34 2 1986 1986 CD cord-269867-k10siwur 34 3 ; ; : cord-269867-k10siwur 34 4 Martineau Martineau NNP cord-269867-k10siwur 34 5 et et NNP cord-269867-k10siwur 34 6 al al NNP cord-269867-k10siwur 34 7 . . NNP cord-269867-k10siwur 34 8 , , , cord-269867-k10siwur 34 9 1992 1992 CD cord-269867-k10siwur 34 10 ; ; : cord-269867-k10siwur 34 11 Khan Khan NNP cord-269867-k10siwur 34 12 et et NNP cord-269867-k10siwur 34 13 al al NNP cord-269867-k10siwur 34 14 . . NNP cord-269867-k10siwur 34 15 , , , cord-269867-k10siwur 34 16 1994 1994 CD cord-269867-k10siwur 34 17 ) ) -RRB- cord-269867-k10siwur 34 18 . . . cord-269867-k10siwur 35 1 A a DT cord-269867-k10siwur 35 2 third third JJ cord-269867-k10siwur 35 3 strategy strategy NN cord-269867-k10siwur 35 4 might may MD cord-269867-k10siwur 35 5 be be VB cord-269867-k10siwur 35 6 the the DT cord-269867-k10siwur 35 7 insertion insertion NN cord-269867-k10siwur 35 8 of of IN cord-269867-k10siwur 35 9 epitopes epitope NNS cord-269867-k10siwur 35 10 in in IN cord-269867-k10siwur 35 11 an an DT cord-269867-k10siwur 35 12 accessible accessible JJ cord-269867-k10siwur 35 13 surface surface NN cord-269867-k10siwur 35 14 region region NN cord-269867-k10siwur 35 15 previously previously RB cord-269867-k10siwur 35 16 observed observe VBD cord-269867-k10siwur 35 17 as as IN cord-269867-k10siwur 35 18 a a DT cord-269867-k10siwur 35 19 natural natural JJ cord-269867-k10siwur 35 20 immunodominant immunodominant JJ cord-269867-k10siwur 35 21 site site NN cord-269867-k10siwur 35 22 on on IN cord-269867-k10siwur 35 23 the the DT cord-269867-k10siwur 35 24 native native JJ cord-269867-k10siwur 35 25 carrier carrier NN cord-269867-k10siwur 35 26 molecule molecule NN cord-269867-k10siwur 35 27 . . . cord-269867-k10siwur 36 1 Here here RB cord-269867-k10siwur 36 2 , , , cord-269867-k10siwur 36 3 we -PRON- PRP cord-269867-k10siwur 36 4 developed develop VBD cord-269867-k10siwur 36 5 these these DT cord-269867-k10siwur 36 6 different different JJ cord-269867-k10siwur 36 7 approaches approach NNS cord-269867-k10siwur 36 8 simultaneously simultaneously RB cord-269867-k10siwur 36 9 by by IN cord-269867-k10siwur 36 10 using use VBG cord-269867-k10siwur 36 11 the the DT cord-269867-k10siwur 36 12 major major JJ cord-269867-k10siwur 36 13 ClpG ClpG NNP cord-269867-k10siwur 36 14 subunit subunit NN cord-269867-k10siwur 36 15 of of IN cord-269867-k10siwur 36 16 the the DT cord-269867-k10siwur 36 17 E. E. NNP cord-269867-k10siwur 36 18 coli coli NNS cord-269867-k10siwur 36 19 CS31A CS31A NNP cord-269867-k10siwur 36 20 fibrillae fibrillae NNP cord-269867-k10siwur 36 21 as as IN cord-269867-k10siwur 36 22 the the DT cord-269867-k10siwur 36 23 carrier carrier NN cord-269867-k10siwur 36 24 protein protein NN cord-269867-k10siwur 36 25 ( ( -LRB- cord-269867-k10siwur 36 26 Bousquet Bousquet NNP cord-269867-k10siwur 36 27 et et NNP cord-269867-k10siwur 36 28 al al NNP cord-269867-k10siwur 36 29 . . NNP cord-269867-k10siwur 36 30 , , , cord-269867-k10siwur 36 31 1994 1994 CD cord-269867-k10siwur 36 32 ; ; : cord-269867-k10siwur 36 33 Der Der NNP cord-269867-k10siwur 36 34 Vartanian Vartanian NNP cord-269867-k10siwur 36 35 et et FW cord-269867-k10siwur 36 36 al al NNP cord-269867-k10siwur 36 37 . . NNP cord-269867-k10siwur 36 38 , , , cord-269867-k10siwur 36 39 1994 1994 CD cord-269867-k10siwur 36 40 ) ) -RRB- cord-269867-k10siwur 36 41 , , , cord-269867-k10siwur 36 42 and and CC cord-269867-k10siwur 36 43 two two CD cord-269867-k10siwur 36 44 B b NN cord-269867-k10siwur 36 45 - - HYPH cord-269867-k10siwur 36 46 cell cell NN cord-269867-k10siwur 36 47 epitopes epitope NNS cord-269867-k10siwur 36 48 from from IN cord-269867-k10siwur 36 49 TGEV TGEV NNP cord-269867-k10siwur 36 50 consisting consist VBG cord-269867-k10siwur 36 51 of of IN cord-269867-k10siwur 36 52 the the DT cord-269867-k10siwur 36 53 site site NN cord-269867-k10siwur 36 54 C C NNP cord-269867-k10siwur 36 55 ( ( -LRB- cord-269867-k10siwur 36 56 aa aa NNP cord-269867-k10siwur 36 57 363 363 CD cord-269867-k10siwur 36 58 - - HYPH cord-269867-k10siwur 36 59 371 371 CD cord-269867-k10siwur 36 60 ) ) -RRB- cord-269867-k10siwur 36 61 and and CC cord-269867-k10siwur 37 1 site site NN cord-269867-k10siwur 37 2 A a NN cord-269867-k10siwur 37 3 ( ( -LRB- cord-269867-k10siwur 37 4 aa aa NNP cord-269867-k10siwur 37 5 522 522 CD cord-269867-k10siwur 37 6 - - HYPH cord-269867-k10siwur 37 7 531 531 CD cord-269867-k10siwur 37 8 ) ) -RRB- cord-269867-k10siwur 37 9 of of IN cord-269867-k10siwur 37 10 TGEV TGEV NNP cord-269867-k10siwur 37 11 - - HYPH cord-269867-k10siwur 37 12 S S NNP cord-269867-k10siwur 37 13 as as IN cord-269867-k10siwur 37 14 the the DT cord-269867-k10siwur 37 15 foreign foreign JJ cord-269867-k10siwur 37 16 antigenic antigenic JJ cord-269867-k10siwur 37 17 determinants determinant NNS cord-269867-k10siwur 37 18 ( ( -LRB- cord-269867-k10siwur 37 19 Delmas Delmas NNP cord-269867-k10siwur 37 20 et et NNP cord-269867-k10siwur 37 21 al al NNP cord-269867-k10siwur 37 22 . . NNP cord-269867-k10siwur 37 23 , , , cord-269867-k10siwur 37 24 1990 1990 CD cord-269867-k10siwur 37 25 ; ; : cord-269867-k10siwur 37 26 Gebauer Gebauer NNP cord-269867-k10siwur 37 27 et et NNP cord-269867-k10siwur 37 28 al al NNP cord-269867-k10siwur 37 29 . . NNP cord-269867-k10siwur 37 30 , , , cord-269867-k10siwur 37 31 1991 1991 CD cord-269867-k10siwur 37 32 ) ) -RRB- cord-269867-k10siwur 37 33 . . . cord-269867-k10siwur 38 1 The the DT cord-269867-k10siwur 38 2 continuous continuous JJ cord-269867-k10siwur 38 3 site site NN cord-269867-k10siwur 38 4 C c NN cord-269867-k10siwur 38 5 elicits elicit VBZ cord-269867-k10siwur 38 6 neutralizing neutralize VBG cord-269867-k10siwur 38 7 Ab Ab NNP cord-269867-k10siwur 38 8 and and CC cord-269867-k10siwur 38 9 the the DT cord-269867-k10siwur 38 10 site site NN cord-269867-k10siwur 39 1 A a DT cord-269867-k10siwur 39 2 is be VBZ cord-269867-k10siwur 39 3 part part NN cord-269867-k10siwur 39 4 of of IN cord-269867-k10siwur 39 5 highly highly RB cord-269867-k10siwur 39 6 immunogenic immunogenic JJ cord-269867-k10siwur 39 7 conformational conformational JJ cord-269867-k10siwur 39 8 antigenic antigenic JJ cord-269867-k10siwur 39 9 region region NN cord-269867-k10siwur 39 10 ( ( -LRB- cord-269867-k10siwur 39 11 Delmas Delmas NNP cord-269867-k10siwur 39 12 et et FW cord-269867-k10siwur 39 13 al al NNP cord-269867-k10siwur 39 14 . . . cord-269867-k10siwur 40 1 , , , cord-269867-k10siwur 40 2 1990 1990 CD cord-269867-k10siwur 40 3 ; ; : cord-269867-k10siwur 40 4 Correa Correa NNP cord-269867-k10siwur 40 5 et et NNP cord-269867-k10siwur 40 6 al al NNP cord-269867-k10siwur 40 7 . . NNP cord-269867-k10siwur 40 8 , , , cord-269867-k10siwur 40 9 1990 1990 CD cord-269867-k10siwur 40 10 ) ) -RRB- cord-269867-k10siwur 40 11 . . . cord-269867-k10siwur 41 1 TGEV TGEV NNP cord-269867-k10siwur 42 1 -A -A : cord-269867-k10siwur 42 2 or or CC cord-269867-k10siwur 42 3 TGEV TGEV NNP cord-269867-k10siwur 42 4 - - HYPH cord-269867-k10siwur 42 5 C C NNP cord-269867-k10siwur 42 6 inserted insert VBD cord-269867-k10siwur 42 7 in in IN cord-269867-k10siwur 42 8 the the DT cord-269867-k10siwur 42 9 position position NN cord-269867-k10siwur 42 10 aa aa NNP cord-269867-k10siwur 42 11 202 202 CD cord-269867-k10siwur 42 12 - - HYPH cord-269867-k10siwur 42 13 218 218 CD cord-269867-k10siwur 42 14 of of IN cord-269867-k10siwur 42 15 CIpG CIpG NNPS cord-269867-k10siwur 42 16 was be VBD cord-269867-k10siwur 42 17 previously previously RB cord-269867-k10siwur 42 18 shown show VBN cord-269867-k10siwur 42 19 to to TO cord-269867-k10siwur 42 20 induce induce VB cord-269867-k10siwur 42 21 a a DT cord-269867-k10siwur 42 22 low low JJ cord-269867-k10siwur 42 23 neutralizing neutralize VBG cord-269867-k10siwur 42 24 Ab Ab NNP cord-269867-k10siwur 42 25 response response NN cord-269867-k10siwur 42 26 in in IN cord-269867-k10siwur 42 27 mice mouse NNS cord-269867-k10siwur 43 1 ( ( -LRB- cord-269867-k10siwur 43 2 Bousquet Bousquet NNP cord-269867-k10siwur 43 3 et et NNP cord-269867-k10siwur 43 4 al al NNP cord-269867-k10siwur 43 5 . . NNP cord-269867-k10siwur 43 6 , , , cord-269867-k10siwur 43 7 1994 1994 CD cord-269867-k10siwur 43 8 ) ) -RRB- cord-269867-k10siwur 43 9 . . . cord-269867-k10siwur 44 1 The the DT cord-269867-k10siwur 44 2 aim aim NN cord-269867-k10siwur 44 3 of of IN cord-269867-k10siwur 44 4 this this DT cord-269867-k10siwur 44 5 work work NN cord-269867-k10siwur 44 6 was be VBD cord-269867-k10siwur 44 7 to to TO cord-269867-k10siwur 44 8 explore explore VB cord-269867-k10siwur 44 9 recombinant recombinant JJ cord-269867-k10siwur 44 10 ClpG::TGEV ClpG::TGEV NNS cord-269867-k10siwur 44 11 proteins protein NNS cord-269867-k10siwur 44 12 for for IN cord-269867-k10siwur 44 13 the the DT cord-269867-k10siwur 44 14 display display NN cord-269867-k10siwur 44 15 of of IN cord-269867-k10siwur 44 16 different different JJ cord-269867-k10siwur 44 17 combinations combination NNS cord-269867-k10siwur 44 18 of of IN cord-269867-k10siwur 44 19 the the DT cord-269867-k10siwur 44 20 two two CD cord-269867-k10siwur 44 21 TGEV TGEV NNP cord-269867-k10siwur 44 22 peptides peptide NNS cord-269867-k10siwur 44 23 in in IN cord-269867-k10siwur 44 24 particular particular JJ cord-269867-k10siwur 44 25 contexts context NNS cord-269867-k10siwur 44 26 that that WDT cord-269867-k10siwur 44 27 could could MD cord-269867-k10siwur 44 28 result result VB cord-269867-k10siwur 44 29 in in IN cord-269867-k10siwur 44 30 improved improved JJ cord-269867-k10siwur 44 31 epitope epitope NN cord-269867-k10siwur 44 32 performance performance NN cord-269867-k10siwur 44 33 , , , cord-269867-k10siwur 44 34 and and CC cord-269867-k10siwur 44 35 thus thus RB cord-269867-k10siwur 44 36 allow allow VB cord-269867-k10siwur 44 37 the the DT cord-269867-k10siwur 44 38 design design NN cord-269867-k10siwur 44 39 of of IN cord-269867-k10siwur 44 40 vaccine vaccine NN cord-269867-k10siwur 44 41 antigens antigen NNS cord-269867-k10siwur 44 42 . . . cord-269867-k10siwur 45 1 For for IN cord-269867-k10siwur 45 2 this this DT cord-269867-k10siwur 45 3 purpose purpose NN cord-269867-k10siwur 45 4 , , , cord-269867-k10siwur 45 5 we -PRON- PRP cord-269867-k10siwur 45 6 examine examine VBP cord-269867-k10siwur 45 7 the the DT cord-269867-k10siwur 45 8 permissivity permissivity NN cord-269867-k10siwur 46 1 ( ( -LRB- cord-269867-k10siwur 46 2 Charbit Charbit NNP cord-269867-k10siwur 46 3 et et NNP cord-269867-k10siwur 46 4 al al NNP cord-269867-k10siwur 46 5 . . NNP cord-269867-k10siwur 46 6 , , , cord-269867-k10siwur 46 7 1991 1991 CD cord-269867-k10siwur 46 8 ) ) -RRB- cord-269867-k10siwur 47 1 Fig Fig NNP cord-269867-k10siwur 47 2 . . NNP cord-269867-k10siwur 47 3 2 2 CD cord-269867-k10siwur 47 4 . . . cord-269867-k10siwur 48 1 Foreign foreign JJ cord-269867-k10siwur 48 2 sequences sequence NNS cord-269867-k10siwur 48 3 used use VBN cord-269867-k10siwur 48 4 in in IN cord-269867-k10siwur 48 5 this this DT cord-269867-k10siwur 48 6 study study NN cord-269867-k10siwur 48 7 for for IN cord-269867-k10siwur 48 8 the the DT cord-269867-k10siwur 48 9 construction construction NN cord-269867-k10siwur 48 10 of of IN cord-269867-k10siwur 48 11 the the DT cord-269867-k10siwur 48 12 chimeric chimeric JJ cord-269867-k10siwur 48 13 clpG clpG NNS cord-269867-k10siwur 48 14 genes gene NNS cord-269867-k10siwur 48 15 . . . cord-269867-k10siwur 49 1 The the DT cord-269867-k10siwur 49 2 six six CD cord-269867-k10siwur 49 3 double double RB cord-269867-k10siwur 49 4 - - HYPH cord-269867-k10siwur 49 5 stranded strand VBN cord-269867-k10siwur 49 6 synthetic synthetic JJ cord-269867-k10siwur 49 7 oligos oligo NNS cord-269867-k10siwur 49 8 coding code VBG cord-269867-k10siwur 49 9 for for IN cord-269867-k10siwur 49 10 TGEV TGEV NNP cord-269867-k10siwur 49 11 - - HYPH cord-269867-k10siwur 49 12 C C NNP cord-269867-k10siwur 49 13 ( ( -LRB- cord-269867-k10siwur 49 14 oligos oligo NNS cord-269867-k10siwur 49 15 # # NN cord-269867-k10siwur 49 16 l l NN cord-269867-k10siwur 49 17 , , , cord-269867-k10siwur 49 18 # # $ cord-269867-k10siwur 49 19 4 4 CD cord-269867-k10siwur 49 20 and and CC cord-269867-k10siwur 49 21 # # $ cord-269867-k10siwur 49 22 5 5 CD cord-269867-k10siwur 49 23 ) ) -RRB- cord-269867-k10siwur 49 24 and and CC cord-269867-k10siwur 50 1 TGEV TGEV NNP cord-269867-k10siwur 50 2 - - HYPH cord-269867-k10siwur 50 3 A a NN cord-269867-k10siwur 50 4 ( ( -LRB- cord-269867-k10siwur 50 5 oligos oligo NNS cord-269867-k10siwur 50 6 # # $ cord-269867-k10siwur 50 7 2 2 CD cord-269867-k10siwur 50 8 , , , cord-269867-k10siwur 50 9 # # $ cord-269867-k10siwur 50 10 3 3 CD cord-269867-k10siwur 50 11 and and CC cord-269867-k10siwur 50 12 # # $ cord-269867-k10siwur 50 13 6 6 CD cord-269867-k10siwur 50 14 ) ) -RRB- cord-269867-k10siwur 50 15 of of IN cord-269867-k10siwur 50 16 TGEV TGEV NNP cord-269867-k10siwur 50 17 - - HYPH cord-269867-k10siwur 50 18 S S NNP cord-269867-k10siwur 50 19 of of IN cord-269867-k10siwur 50 20 the the DT cord-269867-k10siwur 50 21 porcine porcine JJ cord-269867-k10siwur 50 22 Purdue Purdue NNP cord-269867-k10siwur 50 23 - - HYPH cord-269867-k10siwur 50 24 ll5 ll5 NNP cord-269867-k10siwur 50 25 strain strain NN cord-269867-k10siwur 50 26 of of IN cord-269867-k10siwur 50 27 TGEV TGEV NNS cord-269867-k10siwur 50 28 are be VBP cord-269867-k10siwur 50 29 shown show VBN cord-269867-k10siwur 50 30 . . . cord-269867-k10siwur 51 1 The the DT cord-269867-k10siwur 51 2 bolded bolded JJ cord-269867-k10siwur 51 3 aa aa NN cord-269867-k10siwur 51 4 residues residue NNS cord-269867-k10siwur 51 5 correspond correspond VBP cord-269867-k10siwur 51 6 to to IN cord-269867-k10siwur 51 7 the the DT cord-269867-k10siwur 51 8 residues residue NNS cord-269867-k10siwur 51 9 361 361 CD cord-269867-k10siwur 51 10 - - HYPH cord-269867-k10siwur 51 11 371 371 CD cord-269867-k10siwur 51 12 and and CC cord-269867-k10siwur 51 13 522 522 CD cord-269867-k10siwur 51 14 - - SYM cord-269867-k10siwur 51 15 531 531 CD cord-269867-k10siwur 51 16 of of IN cord-269867-k10siwur 51 17 TGEV TGEV NNP cord-269867-k10siwur 51 18 - - HYPH cord-269867-k10siwur 51 19 S S NNP cord-269867-k10siwur 51 20 , , , cord-269867-k10siwur 51 21 spanning span VBG cord-269867-k10siwur 51 22 the the DT cord-269867-k10siwur 51 23 sites site NNS cord-269867-k10siwur 51 24 C c NN cord-269867-k10siwur 51 25 and and CC cord-269867-k10siwur 51 26 A A NNP cord-269867-k10siwur 51 27 , , , cord-269867-k10siwur 51 28 respectively respectively RB cord-269867-k10siwur 51 29 . . . cord-269867-k10siwur 52 1 Restriction restriction NN cord-269867-k10siwur 52 2 sites site NNS cord-269867-k10siwur 52 3 are be VBP cord-269867-k10siwur 52 4 underlined underline VBN cord-269867-k10siwur 52 5 . . . cord-269867-k10siwur 53 1 introducing introduce VBG cord-269867-k10siwur 53 2 TGEV TGEV NNP cord-269867-k10siwur 53 3 - - HYPH cord-269867-k10siwur 53 4 C C NNP cord-269867-k10siwur 53 5 alone alone RB cord-269867-k10siwur 53 6 or or CC cord-269867-k10siwur 53 7 in in IN cord-269867-k10siwur 53 8 tandem tandem NN cord-269867-k10siwur 53 9 with with IN cord-269867-k10siwur 53 10 TGEV TGEV NNP cord-269867-k10siwur 53 11 - - HYPH cord-269867-k10siwur 53 12 A a NN cord-269867-k10siwur 53 13 in in IN cord-269867-k10siwur 53 14 these these DT cord-269867-k10siwur 53 15 sites site NNS cord-269867-k10siwur 53 16 and and CC cord-269867-k10siwur 53 17 by by IN cord-269867-k10siwur 53 18 investigating investigate VBG cord-269867-k10siwur 53 19 the the DT cord-269867-k10siwur 53 20 influence influence NN cord-269867-k10siwur 53 21 of of IN cord-269867-k10siwur 53 22 the the DT cord-269867-k10siwur 53 23 resulting result VBG cord-269867-k10siwur 53 24 modifications modification NNS cord-269867-k10siwur 53 25 on on IN cord-269867-k10siwur 53 26 the the DT cord-269867-k10siwur 53 27 expression expression NN cord-269867-k10siwur 53 28 of of IN cord-269867-k10siwur 53 29 CS31A CS31A NNP cord-269867-k10siwur 53 30 fibrillae fibrillae NNP cord-269867-k10siwur 53 31 . . . cord-269867-k10siwur 54 1 We -PRON- PRP cord-269867-k10siwur 54 2 discuss discuss VBP cord-269867-k10siwur 54 3 the the DT cord-269867-k10siwur 54 4 nature nature NN cord-269867-k10siwur 54 5 of of IN cord-269867-k10siwur 54 6 permissive permissive JJ cord-269867-k10siwur 54 7 sites site NNS cord-269867-k10siwur 54 8 and and CC cord-269867-k10siwur 54 9 the the DT cord-269867-k10siwur 54 10 positioning positioning NN cord-269867-k10siwur 54 11 and and CC cord-269867-k10siwur 54 12 tandem tandem NN cord-269867-k10siwur 54 13 insertion insertion NN cord-269867-k10siwur 54 14 effects effect NNS cord-269867-k10siwur 54 15 of of IN cord-269867-k10siwur 54 16 the the DT cord-269867-k10siwur 54 17 epitopes epitope NNS cord-269867-k10siwur 54 18 . . . cord-269867-k10siwur 55 1 Twelve twelve CD cord-269867-k10siwur 55 2 sites site NNS cord-269867-k10siwur 55 3 within within IN cord-269867-k10siwur 55 4 ClpG ClpG NNP cord-269867-k10siwur 55 5 were be VBD cord-269867-k10siwur 55 6 selected select VBN cord-269867-k10siwur 55 7 for for IN cord-269867-k10siwur 55 8 insertion insertion NN cord-269867-k10siwur 55 9 of of IN cord-269867-k10siwur 55 10 TGEV TGEV NNP cord-269867-k10siwur 55 11 peptides peptide NNS cord-269867-k10siwur 56 1 ( ( -LRB- cord-269867-k10siwur 56 2 Fig Fig NNP cord-269867-k10siwur 56 3 . . NNP cord-269867-k10siwur 56 4 1 1 CD cord-269867-k10siwur 56 5 ) ) -RRB- cord-269867-k10siwur 56 6 . . . cord-269867-k10siwur 57 1 One one CD cord-269867-k10siwur 57 2 of of IN cord-269867-k10siwur 57 3 them -PRON- PRP cord-269867-k10siwur 57 4 is be VBZ cord-269867-k10siwur 57 5 located locate VBN cord-269867-k10siwur 57 6 at at IN cord-269867-k10siwur 57 7 the the DT cord-269867-k10siwur 57 8 N n CD cord-269867-k10siwur 57 9 terminfis terminfis NNP cord-269867-k10siwur 57 10 ( ( -LRB- cord-269867-k10siwur 57 11 Fig Fig NNP cord-269867-k10siwur 57 12 . . . cord-269867-k10siwur 57 13 1B 1B NNP cord-269867-k10siwur 57 14 ) ) -RRB- cord-269867-k10siwur 57 15 , , , cord-269867-k10siwur 57 16 and and CC cord-269867-k10siwur 57 17 the the DT cord-269867-k10siwur 57 18 others other NNS cord-269867-k10siwur 57 19 are be VBP cord-269867-k10siwur 57 20 distributed distribute VBN cord-269867-k10siwur 57 21 along along IN cord-269867-k10siwur 57 22 the the DT cord-269867-k10siwur 57 23 aa aa NNP cord-269867-k10siwur 57 24 182 182 CD cord-269867-k10siwur 57 25 - - SYM cord-269867-k10siwur 57 26 218 218 CD cord-269867-k10siwur 57 27 region region NN cord-269867-k10siwur 57 28 ( ( -LRB- cord-269867-k10siwur 57 29 Fig Fig NNP cord-269867-k10siwur 57 30 . . . cord-269867-k10siwur 57 31 1C 1C NNP cord-269867-k10siwur 57 32 ) ) -RRB- cord-269867-k10siwur 57 33 . . . cord-269867-k10siwur 58 1 This this DT cord-269867-k10siwur 58 2 region region NN cord-269867-k10siwur 58 3 contains contain VBZ cord-269867-k10siwur 58 4 a a DT cord-269867-k10siwur 58 5 variable variable JJ cord-269867-k10siwur 58 6 flexible flexible JJ cord-269867-k10siwur 58 7 loop loop NN cord-269867-k10siwur 58 8 structure structure NN cord-269867-k10siwur 58 9 ( ( -LRB- cord-269867-k10siwur 58 10 aa aa NNP cord-269867-k10siwur 58 11 190 190 CD cord-269867-k10siwur 58 12 - - SYM cord-269867-k10siwur 58 13 217 217 CD cord-269867-k10siwur 58 14 ) ) -RRB- cord-269867-k10siwur 58 15 carrying carry VBG cord-269867-k10siwur 58 16 a a DT cord-269867-k10siwur 58 17 hydrophilic hydrophilic JJ cord-269867-k10siwur 58 18 domain domain NN cord-269867-k10siwur 58 19 and and CC cord-269867-k10siwur 58 20 several several JJ cord-269867-k10siwur 58 21 accessible accessible JJ cord-269867-k10siwur 58 22 continuous continuous JJ cord-269867-k10siwur 58 23 immunodominant immunodominant JJ cord-269867-k10siwur 58 24 epitopes epitope NNS cord-269867-k10siwur 58 25 , , , cord-269867-k10siwur 58 26 one one CD cord-269867-k10siwur 58 27 of of IN cord-269867-k10siwur 58 28 which which WDT cord-269867-k10siwur 58 29 ( ( -LRB- cord-269867-k10siwur 58 30 aa aa NNP cord-269867-k10siwur 58 31 189 189 CD cord-269867-k10siwur 58 32 - - SYM cord-269867-k10siwur 58 33 194 194 CD cord-269867-k10siwur 58 34 ) ) -RRB- cord-269867-k10siwur 58 35 is be VBZ cord-269867-k10siwur 58 36 exposed expose VBN cord-269867-k10siwur 58 37 on on IN cord-269867-k10siwur 58 38 the the DT cord-269867-k10siwur 58 39 native native JJ cord-269867-k10siwur 58 40 ClpG ClpG NNP cord-269867-k10siwur 58 41 subunit subunit NN cord-269867-k10siwur 58 42 at at IN cord-269867-k10siwur 58 43 the the DT cord-269867-k10siwur 58 44 surface surface NN cord-269867-k10siwur 58 45 of of IN cord-269867-k10siwur 58 46 the the DT cord-269867-k10siwur 58 47 polymeric polymeric JJ cord-269867-k10siwur 58 48 CS31A cs31a NN cord-269867-k10siwur 58 49 fibrillae fibrillae NNP cord-269867-k10siwur 58 50 ( ( -LRB- cord-269867-k10siwur 58 51 Fig Fig NNP cord-269867-k10siwur 58 52 . . . cord-269867-k10siwur 58 53 1C 1C NNP cord-269867-k10siwur 58 54 ) ) -RRB- cord-269867-k10siwur 58 55 . . . cord-269867-k10siwur 59 1 For for IN cord-269867-k10siwur 59 2 these these DT cord-269867-k10siwur 59 3 reasons reason NNS cord-269867-k10siwur 59 4 , , , cord-269867-k10siwur 59 5 we -PRON- PRP cord-269867-k10siwur 59 6 hypothesized hypothesize VBD cord-269867-k10siwur 59 7 that that IN cord-269867-k10siwur 59 8 the the DT cord-269867-k10siwur 59 9 region region NN cord-269867-k10siwur 59 10 aa aa NNP cord-269867-k10siwur 59 11 182 182 CD cord-269867-k10siwur 59 12 - - HYPH cord-269867-k10siwur 59 13 218 218 CD cord-269867-k10siwur 59 14 is be VBZ cord-269867-k10siwur 59 15 naturally naturally RB cord-269867-k10siwur 59 16 favorable favorable JJ cord-269867-k10siwur 59 17 to to IN cord-269867-k10siwur 59 18 the the DT cord-269867-k10siwur 59 19 presentation presentation NN cord-269867-k10siwur 59 20 of of IN cord-269867-k10siwur 59 21 the the DT cord-269867-k10siwur 59 22 TGEV TGEV NNP cord-269867-k10siwur 59 23 epitopes epitope NNS cord-269867-k10siwur 59 24 . . . cord-269867-k10siwur 60 1 To to TO cord-269867-k10siwur 60 2 construct construct VB cord-269867-k10siwur 60 3 insertion insertion NN cord-269867-k10siwur 60 4 p]asmid p]asmid NN cord-269867-k10siwur 60 5 vectors vector NNS cord-269867-k10siwur 60 6 as as IN cord-269867-k10siwur 60 7 a a DT cord-269867-k10siwur 60 8 preliminary preliminary JJ cord-269867-k10siwur 60 9 step step NN cord-269867-k10siwur 60 10 for for IN cord-269867-k10siwur 60 11 viral viral JJ cord-269867-k10siwur 60 12 epitopes epitope NNS cord-269867-k10siwur 60 13 insertion insertion NN cord-269867-k10siwur 60 14 in in IN cord-269867-k10siwur 60 15 the the DT cord-269867-k10siwur 60 16 selected select VBN cord-269867-k10siwur 60 17 regions region NNS cord-269867-k10siwur 60 18 aa aa NNP cord-269867-k10siwur 60 19 190 190 CD cord-269867-k10siwur 60 20 - - SYM cord-269867-k10siwur 60 21 198 198 CD cord-269867-k10siwur 60 22 and and CC cord-269867-k10siwur 60 23 202 202 CD cord-269867-k10siwur 60 24 - - SYM cord-269867-k10siwur 60 25 218 218 CD cord-269867-k10siwur 60 26 of of IN cord-269867-k10siwur 60 27 ClpG ClpG NNP cord-269867-k10siwur 60 28 ( ( -LRB- cord-269867-k10siwur 60 29 Fig Fig NNP cord-269867-k10siwur 60 30 . . . cord-269867-k10siwur 60 31 1C 1C NNP cord-269867-k10siwur 60 32 ) ) -RRB- cord-269867-k10siwur 60 33 , , , cord-269867-k10siwur 60 34 the the DT cord-269867-k10siwur 60 35 corresponding correspond VBG cord-269867-k10siwur 60 36 clpG clpG NNS cord-269867-k10siwur 60 37 sequences sequence NNS cord-269867-k10siwur 60 38 were be VBD cord-269867-k10siwur 60 39 submitted submit VBN cord-269867-k10siwur 60 40 to to IN cord-269867-k10siwur 60 41 oligodeoxyribonucleotide oligodeoxyribonucleotide NNP cord-269867-k10siwur 60 42 ( ( -LRB- cord-269867-k10siwur 60 43 oligo oligo NNP cord-269867-k10siwur 60 44 ) ) -RRB- cord-269867-k10siwur 60 45 site site NN cord-269867-k10siwur 60 46 - - HYPH cord-269867-k10siwur 60 47 directed direct VBN cord-269867-k10siwur 60 48 mutagenesis mutagenesis NN cord-269867-k10siwur 60 49 to to TO cord-269867-k10siwur 60 50 create create VB cord-269867-k10siwur 60 51 unique unique JJ cord-269867-k10siwur 60 52 restriction restriction NN cord-269867-k10siwur 60 53 sites site NNS cord-269867-k10siwur 60 54 at at IN cord-269867-k10siwur 60 55 different different JJ cord-269867-k10siwur 60 56 positions position NNS cord-269867-k10siwur 60 57 within within IN cord-269867-k10siwur 60 58 these these DT cord-269867-k10siwur 60 59 sequences sequence NNS cord-269867-k10siwur 60 60 . . . cord-269867-k10siwur 61 1 Thus thus RB cord-269867-k10siwur 61 2 , , , cord-269867-k10siwur 61 3 a a DT cord-269867-k10siwur 61 4 SpeI SpeI NNP cord-269867-k10siwur 61 5 site site NN cord-269867-k10siwur 61 6 was be VBD cord-269867-k10siwur 61 7 independently independently RB cord-269867-k10siwur 61 8 engineered engineer VBN cord-269867-k10siwur 61 9 after after IN cord-269867-k10siwur 61 10 each each DT cord-269867-k10siwur 61 11 codon codon NN cord-269867-k10siwur 61 12 expressing express VBG cord-269867-k10siwur 61 13 every every DT cord-269867-k10siwur 61 14 one one CD cord-269867-k10siwur 61 15 of of IN cord-269867-k10siwur 61 16 aa aa NNP cord-269867-k10siwur 61 17 composing compose VBG cord-269867-k10siwur 61 18 the the DT cord-269867-k10siwur 61 19 region region NN cord-269867-k10siwur 61 20 aa aa NNP cord-269867-k10siwur 61 21 190 190 CD cord-269867-k10siwur 62 1 -198 -198 NNP cord-269867-k10siwur 62 2 ( ( -LRB- cord-269867-k10siwur 62 3 Fig Fig NNP cord-269867-k10siwur 62 4 . . . cord-269867-k10siwur 62 5 1C 1C NNP cord-269867-k10siwur 62 6 ) ) -RRB- cord-269867-k10siwur 62 7 , , . cord-269867-k10siwur 63 1 resulting result VBG cord-269867-k10siwur 63 2 in in IN cord-269867-k10siwur 63 3 the the DT cord-269867-k10siwur 63 4 addition addition NN cord-269867-k10siwur 63 5 of of IN cord-269867-k10siwur 63 6 the the DT cord-269867-k10siwur 63 7 dipeptide dipeptide JJ cord-269867-k10siwur 63 8 threonine threonine NN cord-269867-k10siwur 63 9 - - HYPH cord-269867-k10siwur 63 10 serine serine NN cord-269867-k10siwur 63 11 ( ( -LRB- cord-269867-k10siwur 63 12 TS TS NNP cord-269867-k10siwur 63 13 ) ) -RRB- cord-269867-k10siwur 63 14 ( ( -LRB- cord-269867-k10siwur 63 15 Fig Fig NNP cord-269867-k10siwur 63 16 . . . cord-269867-k10siwur 63 17 3 3 CD cord-269867-k10siwur 63 18 ) ) -RRB- cord-269867-k10siwur 63 19 . . . cord-269867-k10siwur 64 1 The the DT cord-269867-k10siwur 64 2 aa aa NNP cord-269867-k10siwur 64 3 202 202 CD cord-269867-k10siwur 64 4 - - HYPH cord-269867-k10siwur 64 5 218 218 CD cord-269867-k10siwur 64 6 peptide peptide NN cord-269867-k10siwur 64 7 - - HYPH cord-269867-k10siwur 64 8 encoding encode VBG cord-269867-k10siwur 64 9 sequence sequence NN cord-269867-k10siwur 64 10 was be VBD cord-269867-k10siwur 64 11 Y y NN cord-269867-k10siwur 64 12 - - HYPH cord-269867-k10siwur 64 13 ended end VBN cord-269867-k10siwur 64 14 by by IN cord-269867-k10siwur 64 15 a a DT cord-269867-k10siwur 64 16 SpeI SpeI NNP cord-269867-k10siwur 64 17 site site NN cord-269867-k10siwur 64 18 and and CC cord-269867-k10siwur 64 19 Y Y NNP cord-269867-k10siwur 64 20 - - HYPH cord-269867-k10siwur 64 21 ended end VBN cord-269867-k10siwur 64 22 by by IN cord-269867-k10siwur 64 23 a a DT cord-269867-k10siwur 64 24 BgllI BgllI NNP cord-269867-k10siwur 64 25 site site NN cord-269867-k10siwur 64 26 after after IN cord-269867-k10siwur 64 27 two two CD cord-269867-k10siwur 64 28 rounds round NNS cord-269867-k10siwur 64 29 of of IN cord-269867-k10siwur 64 30 mutagenesis mutagenesis NN cord-269867-k10siwur 64 31 which which WDT cord-269867-k10siwur 64 32 induced induce VBD cord-269867-k10siwur 64 33 changes change NNS cord-269867-k10siwur 64 34 in in IN cord-269867-k10siwur 64 35 ClpG ClpG NNP cord-269867-k10siwur 64 36 ( ( -LRB- cord-269867-k10siwur 64 37 Fig Fig NNP cord-269867-k10siwur 64 38 . . . cord-269867-k10siwur 64 39 1C 1C NNP cord-269867-k10siwur 64 40 , , , cord-269867-k10siwur 64 41 mutants mutant NNS cord-269867-k10siwur 64 42 CIpG202 CIpG202 NNP cord-269867-k10siwur 64 43 and and CC cord-269867-k10siwur 64 44 ClpG420 ClpG420 NNP cord-269867-k10siwur 64 45 ) ) -RRB- cord-269867-k10siwur 64 46 ; ; : cord-269867-k10siwur 64 47 the the DT cord-269867-k10siwur 64 48 first first JJ cord-269867-k10siwur 64 49 round round NN cord-269867-k10siwur 64 50 allows allow VBZ cord-269867-k10siwur 64 51 insertions insertion NNS cord-269867-k10siwur 64 52 into into IN cord-269867-k10siwur 64 53 the the DT cord-269867-k10siwur 64 54 site site NN cord-269867-k10siwur 64 55 aa aa NNP cord-269867-k10siwur 64 56 202 202 CD cord-269867-k10siwur 64 57 - - HYPH cord-269867-k10siwur 64 58 204 204 CD cord-269867-k10siwur 64 59 and and CC cord-269867-k10siwur 64 60 the the DT cord-269867-k10siwur 64 61 second second JJ cord-269867-k10siwur 64 62 the the DT cord-269867-k10siwur 64 63 replacement replacement NN cord-269867-k10siwur 64 64 of of IN cord-269867-k10siwur 64 65 the the DT cord-269867-k10siwur 64 66 region region NN cord-269867-k10siwur 64 67 aa aa NNP cord-269867-k10siwur 64 68 203 203 NNP cord-269867-k10siwur 64 69 - - HYPH cord-269867-k10siwur 64 70 217 217 CD cord-269867-k10siwur 64 71 . . . cord-269867-k10siwur 65 1 The the DT cord-269867-k10siwur 65 2 strategy strategy NN cord-269867-k10siwur 65 3 of of IN cord-269867-k10siwur 65 4 ClpG::TGEV ClpG::TGEV NNS cord-269867-k10siwur 65 5 hybrids hybrid NNS cord-269867-k10siwur 65 6 construction construction NN cord-269867-k10siwur 65 7 was be VBD cord-269867-k10siwur 65 8 to to TO cord-269867-k10siwur 65 9 use use VB cord-269867-k10siwur 65 10 these these DT cord-269867-k10siwur 65 11 engineered engineer VBN cord-269867-k10siwur 65 12 restriction restriction NN cord-269867-k10siwur 65 13 sites site NNS cord-269867-k10siwur 65 14 , , , cord-269867-k10siwur 65 15 and and CC cord-269867-k10siwur 65 16 two two CD cord-269867-k10siwur 65 17 naturally naturally RB cord-269867-k10siwur 65 18 occurring occur VBG cord-269867-k10siwur 65 19 sites site NNS cord-269867-k10siwur 65 20 , , , cord-269867-k10siwur 65 21 SphI SphI NNP cord-269867-k10siwur 65 22 ( ( -LRB- cord-269867-k10siwur 65 23 Fig Fig NNP cord-269867-k10siwur 65 24 . . . cord-269867-k10siwur 65 25 1B 1b LS cord-269867-k10siwur 65 26 ) ) -RRB- cord-269867-k10siwur 65 27 and and CC cord-269867-k10siwur 65 28 MunI MunI NNP cord-269867-k10siwur 65 29 ( ( -LRB- cord-269867-k10siwur 65 30 Fig Fig NNP cord-269867-k10siwur 65 31 . . . cord-269867-k10siwur 65 32 1C 1C NNP cord-269867-k10siwur 65 33 unique unique JJ cord-269867-k10siwur 65 34 XhoI XhoI NNP cord-269867-k10siwur 65 35 site site NN cord-269867-k10siwur 65 36 with with IN cord-269867-k10siwur 65 37 SpeI SpeI NNP cord-269867-k10siwur 65 38 - - HYPH cord-269867-k10siwur 65 39 compafible compafible JJ cord-269867-k10siwur 65 40 ends end VBZ cord-269867-k10siwur 65 41 into into IN cord-269867-k10siwur 65 42 the the DT cord-269867-k10siwur 65 43 SpeI SpeI NNP cord-269867-k10siwur 65 44 site site NN cord-269867-k10siwur 65 45 of of IN cord-269867-k10siwur 65 46 pPSX6S ppsx6s NN cord-269867-k10siwur 66 1 ( ( -LRB- cord-269867-k10siwur 66 2 see see VB cord-269867-k10siwur 66 3 Fig Fig NNP cord-269867-k10siwur 66 4 . . NNP cord-269867-k10siwur 66 5 1 1 CD cord-269867-k10siwur 66 6 and and CC cord-269867-k10siwur 66 7 its -PRON- PRP$ cord-269867-k10siwur 66 8 legend legend NN cord-269867-k10siwur 66 9 ) ) -RRB- cord-269867-k10siwur 66 10 ; ; : cord-269867-k10siwur 66 11 pGAC326 pGAC326 NNP cord-269867-k10siwur 66 12 and and CC cord-269867-k10siwur 66 13 pGXC326 pGXC326 NNPS cord-269867-k10siwur 66 14 were be VBD cord-269867-k10siwur 66 15 constructed construct VBN cord-269867-k10siwur 66 16 by by IN cord-269867-k10siwur 66 17 inserting insert VBG cord-269867-k10siwur 66 18 the the DT cord-269867-k10siwur 66 19 oligo oligo NN cord-269867-k10siwur 66 20 # # $ cord-269867-k10siwur 66 21 3 3 CD cord-269867-k10siwur 66 22 ( ( -LRB- cord-269867-k10siwur 66 23 Fig fig NN cord-269867-k10siwur 66 24 . . NNP cord-269867-k10siwur 66 25 2 2 LS cord-269867-k10siwur 66 26 ) ) -RRB- cord-269867-k10siwur 66 27 with with IN cord-269867-k10siwur 66 28 Xhol Xhol NNP cord-269867-k10siwur 66 29 - - HYPH cord-269867-k10siwur 66 30 compatible compatible JJ cord-269867-k10siwur 66 31 ends end NNS cord-269867-k10siwur 66 32 into into IN cord-269867-k10siwur 66 33 the the DT cord-269867-k10siwur 66 34 XhoI XhoI NNP cord-269867-k10siwur 66 35 site site NN cord-269867-k10siwur 66 36 of of IN cord-269867-k10siwur 66 37 pGC326 pGC326 NNP cord-269867-k10siwur 66 38 , , , cord-269867-k10siwur 66 39 resulting result VBG cord-269867-k10siwur 66 40 in in IN cord-269867-k10siwur 66 41 the the DT cord-269867-k10siwur 66 42 addition addition NN cord-269867-k10siwur 66 43 of of IN cord-269867-k10siwur 66 44 the the DT cord-269867-k10siwur 66 45 oligo oligo NN cord-269867-k10siwur 66 46 # # $ cord-269867-k10siwur 66 47 3 3 CD cord-269867-k10siwur 66 48 in in IN cord-269867-k10siwur 66 49 the the DT cord-269867-k10siwur 66 50 correct correct JJ cord-269867-k10siwur 66 51 and and CC cord-269867-k10siwur 66 52 in in IN cord-269867-k10siwur 66 53 - - HYPH cord-269867-k10siwur 66 54 frame frame NN cord-269867-k10siwur 66 55 reverse reverse NN cord-269867-k10siwur 66 56 orientations orientation NNS cord-269867-k10siwur 66 57 , , , cord-269867-k10siwur 66 58 respectively respectively RB cord-269867-k10siwur 66 59 ; ; : cord-269867-k10siwur 66 60 pGA102 pGA102 NNS cord-269867-k10siwur 66 61 ( ( -LRB- cord-269867-k10siwur 66 62 Bousquet Bousquet NNP cord-269867-k10siwur 66 63 et et NNP cord-269867-k10siwur 66 64 al al NNP cord-269867-k10siwur 66 65 . . NNP cord-269867-k10siwur 66 66 , , , cord-269867-k10siwur 66 67 1994 1994 CD cord-269867-k10siwur 66 68 ) ) -RRB- cord-269867-k10siwur 66 69 was be VBD cord-269867-k10siwur 66 70 made make VBN cord-269867-k10siwur 66 71 by by IN cord-269867-k10siwur 66 72 replacing replace VBG cord-269867-k10siwur 66 73 the the DT cord-269867-k10siwur 66 74 38-bp 38-bp CD cord-269867-k10siwur 66 75 SpeI SpeI NNP cord-269867-k10siwur 66 76 - - HYPH cord-269867-k10siwur 66 77 BglII BglII NNP cord-269867-k10siwur 66 78 fragment fragment NN cord-269867-k10siwur 66 79 from from IN cord-269867-k10siwur 66 80 pPSX10S ppsx10 NNS cord-269867-k10siwur 66 81 ( ( -LRB- cord-269867-k10siwur 66 82 see see VB cord-269867-k10siwur 66 83 Fig Fig NNP cord-269867-k10siwur 66 84 . . NNP cord-269867-k10siwur 66 85 1 1 CD cord-269867-k10siwur 66 86 and and CC cord-269867-k10siwur 66 87 its -PRON- PRP$ cord-269867-k10siwur 66 88 legend legend NN cord-269867-k10siwur 66 89 ) ) -RRB- cord-269867-k10siwur 66 90 with with IN cord-269867-k10siwur 66 91 oligo oligo NNP cord-269867-k10siwur 66 92 # # $ cord-269867-k10siwur 66 93 6 6 CD cord-269867-k10siwur 67 1 ( ( -LRB- cord-269867-k10siwur 67 2 Fig Fig NNP cord-269867-k10siwur 67 3 . . NNP cord-269867-k10siwur 67 4 2 2 CD cord-269867-k10siwur 67 5 ) ) -RRB- cord-269867-k10siwur 68 1 having have VBG cord-269867-k10siwur 68 2 5'-SpeI 5'-spei CD cord-269867-k10siwur 68 3 and and CC cord-269867-k10siwur 68 4 BgllI-3 BgllI-3 NNP cord-269867-k10siwur 68 5 ' ' POS cord-269867-k10siwur 68 6 compatible compatible JJ cord-269867-k10siwur 68 7 ends end NNS cord-269867-k10siwur 68 8 ; ; : cord-269867-k10siwur 68 9 pGCA102 pGCA102 NNP cord-269867-k10siwur 68 10 was be VBD cord-269867-k10siwur 68 11 constructed construct VBN cord-269867-k10siwur 68 12 by by IN cord-269867-k10siwur 68 13 inserting insert VBG cord-269867-k10siwur 68 14 the the DT cord-269867-k10siwur 68 15 oligo oligo NN cord-269867-k10siwur 68 16 # # $ cord-269867-k10siwur 68 17 5 5 CD cord-269867-k10siwur 68 18 containing contain VBG cord-269867-k10siwur 68 19 unique unique JJ cord-269867-k10siwur 68 20 XhoI XhoI NNP cord-269867-k10siwur 68 21 site site NN cord-269867-k10siwur 68 22 with with IN cord-269867-k10siwur 68 23 SpeI SpeI NNP cord-269867-k10siwur 68 24 - - HYPH cord-269867-k10siwur 68 25 compatible compatible JJ cord-269867-k10siwur 68 26 ends end NNS cord-269867-k10siwur 68 27 into into IN cord-269867-k10siwur 68 28 the the DT cord-269867-k10siwur 68 29 SpeI SpeI NNP cord-269867-k10siwur 68 30 site site NN cord-269867-k10siwur 68 31 of of IN cord-269867-k10siwur 68 32 pGA102 pGA102 NNS cord-269867-k10siwur 68 33 ; ; : cord-269867-k10siwur 68 34 pGisa226 pGisa226 NNP cord-269867-k10siwur 68 35 was be VBD cord-269867-k10siwur 68 36 engineered engineer VBN cord-269867-k10siwur 68 37 by by IN cord-269867-k10siwur 68 38 cloning clone VBG cord-269867-k10siwur 68 39 the the DT cord-269867-k10siwur 68 40 oligo oligo NN cord-269867-k10siwur 68 41 # # $ cord-269867-k10siwur 68 42 1 1 CD cord-269867-k10siwur 69 1 ( ( -LRB- cord-269867-k10siwur 69 2 Fig Fig NNP cord-269867-k10siwur 69 3 . . NNP cord-269867-k10siwur 69 4 2 2 LS cord-269867-k10siwur 69 5 ) ) -RRB- cord-269867-k10siwur 69 6 comprising comprise VBG cord-269867-k10siwur 69 7 unique unique JJ cord-269867-k10siwur 69 8 XhoI XhoI NNP cord-269867-k10siwur 69 9 site site NN cord-269867-k10siwur 69 10 with with IN cord-269867-k10siwur 69 11 SphI SphI NNP cord-269867-k10siwur 69 12 - - HYPH cord-269867-k10siwur 69 13 flanked flank VBN cord-269867-k10siwur 69 14 ends end NNS cord-269867-k10siwur 69 15 into into IN cord-269867-k10siwur 69 16 the the DT cord-269867-k10siwur 69 17 unique unique JJ cord-269867-k10siwur 69 18 SphI SphI NNP cord-269867-k10siwur 69 19 site site NN cord-269867-k10siwur 69 20 of of IN cord-269867-k10siwur 69 21 pDSPH524 pDSPH524 NNP cord-269867-k10siwur 69 22 ; ; : cord-269867-k10siwur 69 23 pGAC524 pGAC524 NNP cord-269867-k10siwur 69 24 was be VBD cord-269867-k10siwur 69 25 obtained obtain VBN cord-269867-k10siwur 69 26 by by IN cord-269867-k10siwur 69 27 inserting insert VBG cord-269867-k10siwur 69 28 the the DT cord-269867-k10siwur 69 29 oligo oligo NN cord-269867-k10siwur 69 30 # # $ cord-269867-k10siwur 69 31 3 3 CD cord-269867-k10siwur 69 32 with with IN cord-269867-k10siwur 69 33 XhoI XhoI NNP cord-269867-k10siwur 69 34 - - HYPH cord-269867-k10siwur 69 35 compatible compatible JJ cord-269867-k10siwur 69 36 ends end NNS cord-269867-k10siwur 69 37 into into IN cord-269867-k10siwur 69 38 the the DT cord-269867-k10siwur 69 39 XhoI XhoI NNP cord-269867-k10siwur 69 40 site site NN cord-269867-k10siwur 69 41 of of IN cord-269867-k10siwur 69 42 pGisa226 pGisa226 NNP cord-269867-k10siwur 69 43 ; ; : cord-269867-k10siwur 69 44 pGCA41155 pGCA41155 NNP cord-269867-k10siwur 69 45 was be VBD cord-269867-k10siwur 69 46 constructed construct VBN cord-269867-k10siwur 69 47 in in IN cord-269867-k10siwur 69 48 two two CD cord-269867-k10siwur 69 49 steps step NNS cord-269867-k10siwur 69 50 from from IN cord-269867-k10siwur 69 51 pDEV41155 pDEV41155 NNP cord-269867-k10siwur 69 52 : : : cord-269867-k10siwur 69 53 in in IN cord-269867-k10siwur 69 54 the the DT cord-269867-k10siwur 69 55 first first JJ cord-269867-k10siwur 69 56 step step NN cord-269867-k10siwur 69 57 , , , cord-269867-k10siwur 69 58 the the DT cord-269867-k10siwur 69 59 oligo oligo NN cord-269867-k10siwur 69 60 # # $ cord-269867-k10siwur 69 61 1 1 CD cord-269867-k10siwur 69 62 including include VBG cord-269867-k10siwur 69 63 unique unique JJ cord-269867-k10siwur 69 64 NsiI NsiI NNP cord-269867-k10siwur 69 65 site site NN cord-269867-k10siwur 69 66 with with IN cord-269867-k10siwur 69 67 SphI SphI NNP cord-269867-k10siwur 69 68 - - HYPH cord-269867-k10siwur 69 69 flanked flank VBN cord-269867-k10siwur 69 70 ends end NNS cord-269867-k10siwur 69 71 was be VBD cord-269867-k10siwur 69 72 cloned clone VBN cord-269867-k10siwur 69 73 into into IN cord-269867-k10siwur 69 74 the the DT cord-269867-k10siwur 69 75 SphI SphI NNP cord-269867-k10siwur 69 76 site site NN cord-269867-k10siwur 69 77 of of IN cord-269867-k10siwur 69 78 clpG clpG NNS cord-269867-k10siwur 69 79 ( ( -LRB- cord-269867-k10siwur 69 80 Fig Fig NNP cord-269867-k10siwur 69 81 . . . cord-269867-k10siwur 69 82 1B 1B NNP cord-269867-k10siwur 69 83 ) ) -RRB- cord-269867-k10siwur 69 84 and and CC cord-269867-k10siwur 69 85 in in IN cord-269867-k10siwur 69 86 the the DT cord-269867-k10siwur 69 87 second second JJ cord-269867-k10siwur 69 88 step step NN cord-269867-k10siwur 69 89 , , , cord-269867-k10siwur 69 90 the the DT cord-269867-k10siwur 69 91 NsiI NsiI NNP cord-269867-k10siwur 69 92 site site NN cord-269867-k10siwur 69 93 in in IN cord-269867-k10siwur 69 94 oligo oligo NNP cord-269867-k10siwur 69 95 # # $ cord-269867-k10siwur 69 96 1 1 CD cord-269867-k10siwur 69 97 allowed allow VBD cord-269867-k10siwur 69 98 the the DT cord-269867-k10siwur 69 99 insertion insertion NN cord-269867-k10siwur 69 100 of of IN cord-269867-k10siwur 69 101 oligo oligo NN cord-269867-k10siwur 69 102 # # $ cord-269867-k10siwur 69 103 2 2 CD cord-269867-k10siwur 70 1 ( ( -LRB- cord-269867-k10siwur 70 2 Fig Fig NNP cord-269867-k10siwur 70 3 . . NNP cord-269867-k10siwur 70 4 2 2 CD cord-269867-k10siwur 70 5 ) ) -RRB- cord-269867-k10siwur 70 6 with with IN cord-269867-k10siwur 70 7 NsiI NsiI NNP cord-269867-k10siwur 70 8 compatible compatible JJ cord-269867-k10siwur 70 9 ends end NNS cord-269867-k10siwur 70 10 downstream downstream RB cord-269867-k10siwur 70 11 of of IN cord-269867-k10siwur 70 12 oligo oligo NN cord-269867-k10siwur 70 13 # # $ cord-269867-k10siwur 70 14 1 1 CD cord-269867-k10siwur 70 15 ; ; : cord-269867-k10siwur 70 16 pCO6 pCO6 NNP cord-269867-k10siwur 70 17 was be VBD cord-269867-k10siwur 70 18 made make VBN cord-269867-k10siwur 70 19 from from IN cord-269867-k10siwur 70 20 pDEV41155 pdev41155 JJ cord-269867-k10siwur 70 21 by by IN cord-269867-k10siwur 70 22 inserting insert VBG cord-269867-k10siwur 70 23 the the DT cord-269867-k10siwur 70 24 oligo oligo NN cord-269867-k10siwur 70 25 # # $ cord-269867-k10siwur 70 26 4 4 CD cord-269867-k10siwur 70 27 ( ( -LRB- cord-269867-k10siwur 70 28 Fig Fig NNP cord-269867-k10siwur 70 29 . . NNP cord-269867-k10siwur 70 30 2 2 CD cord-269867-k10siwur 70 31 ) ) -RRB- cord-269867-k10siwur 70 32 with with IN cord-269867-k10siwur 70 33 MunI MunI NNP cord-269867-k10siwur 70 34 - - HYPH cord-269867-k10siwur 70 35 compatible compatible JJ cord-269867-k10siwur 70 36 ends end NNS cord-269867-k10siwur 70 37 into into IN cord-269867-k10siwur 70 38 the the DT cord-269867-k10siwur 70 39 MunI MunI NNP cord-269867-k10siwur 70 40 site site NN cord-269867-k10siwur 70 41 of of IN cord-269867-k10siwur 70 42 clpG clpG NNS cord-269867-k10siwur 70 43 ( ( -LRB- cord-269867-k10siwur 70 44 Fig Fig NNP cord-269867-k10siwur 70 45 . . . cord-269867-k10siwur 70 46 1C 1C NNP cord-269867-k10siwur 70 47 ) ) -RRB- cord-269867-k10siwur 70 48 ; ; : cord-269867-k10siwur 70 49 pVN1 pvn1 XX cord-269867-k10siwur 70 50 , , , cord-269867-k10siwur 70 51 pNP2 pNP2 NNP cord-269867-k10siwur 70 52 , , , cord-269867-k10siwur 70 53 pPN3 pPN3 NNP cord-269867-k10siwur 70 54 , , , cord-269867-k10siwur 70 55 pNA4 pNA4 NNP cord-269867-k10siwur 70 56 , , , cord-269867-k10siwur 70 57 pAG5 pAG5 NNP cord-269867-k10siwur 70 58 , , , cord-269867-k10siwur 70 59 pGN6 pGN6 NNP cord-269867-k10siwur 70 60 , , , cord-269867-k10siwur 70 61 pNR7 pNR7 NNP cord-269867-k10siwur 70 62 and and CC cord-269867-k10siwur 70 63 pRG8 pRG8 NNP cord-269867-k10siwur 70 64 were be VBD cord-269867-k10siwur 70 65 constructed construct VBN cord-269867-k10siwur 70 66 from from IN cord-269867-k10siwur 70 67 pDEV41155 pDEV41155 NNP cord-269867-k10siwur 70 68 as as IN cord-269867-k10siwur 70 69 follows follow VBZ cord-269867-k10siwur 70 70 : : : cord-269867-k10siwur 70 71 a a DT cord-269867-k10siwur 70 72 SpeI SpeI NNP cord-269867-k10siwur 70 73 site site NN cord-269867-k10siwur 70 74 within within IN cord-269867-k10siwur 70 75 clpG clpG NNS cord-269867-k10siwur 70 76 was be VBD cord-269867-k10siwur 70 77 introduced introduce VBN cord-269867-k10siwur 70 78 after after IN cord-269867-k10siwur 70 79 each each DT cord-269867-k10siwur 70 80 codon codon NN cord-269867-k10siwur 70 81 expressing express VBG cord-269867-k10siwur 70 82 every every DT cord-269867-k10siwur 70 83 one one CD cord-269867-k10siwur 70 84 of of IN cord-269867-k10siwur 70 85 aa aa NNP cord-269867-k10siwur 70 86 residues residue NNS cord-269867-k10siwur 70 87 covering cover VBG cord-269867-k10siwur 70 88 the the DT cord-269867-k10siwur 70 89 region region NN cord-269867-k10siwur 70 90 190 190 CD cord-269867-k10siwur 70 91 - - SYM cord-269867-k10siwur 70 92 197 197 CD cord-269867-k10siwur 70 93 of of IN cord-269867-k10siwur 70 94 CIpG CIpG NNPS cord-269867-k10siwur 71 1 ( ( -LRB- cord-269867-k10siwur 71 2 Fig Fig NNP cord-269867-k10siwur 71 3 . . . cord-269867-k10siwur 71 4 1C 1C NNP cord-269867-k10siwur 71 5 ) ) -RRB- cord-269867-k10siwur 71 6 by by IN cord-269867-k10siwur 71 7 in in FW cord-269867-k10siwur 71 8 vitro vitro FW cord-269867-k10siwur 71 9 mutagenesis mutagenesis NN cord-269867-k10siwur 71 10 using use VBG cord-269867-k10siwur 71 11 oligos oligos NNP cord-269867-k10siwur 71 12 VN1 VN1 NNP cord-269867-k10siwur 71 13 , , , cord-269867-k10siwur 71 14 NP2 np2 JJ cord-269867-k10siwur 71 15 , , , cord-269867-k10siwur 71 16 PN3 pn3 NN cord-269867-k10siwur 71 17 , , , cord-269867-k10siwur 71 18 NA4 NA4 NNP cord-269867-k10siwur 71 19 , , , cord-269867-k10siwur 71 20 AG5 AG5 NNP cord-269867-k10siwur 71 21 , , , cord-269867-k10siwur 71 22 GN6 GN6 NNP cord-269867-k10siwur 71 23 , , , cord-269867-k10siwur 71 24 NR7 NR7 NNP cord-269867-k10siwur 71 25 and and CC cord-269867-k10siwur 71 26 RG8 RG8 NNP cord-269867-k10siwur 71 27 ( ( -LRB- cord-269867-k10siwur 71 28 Fig Fig NNP cord-269867-k10siwur 71 29 . . . cord-269867-k10siwur 71 30 1 1 CD cord-269867-k10siwur 71 31 legend legend NN cord-269867-k10siwur 71 32 ) ) -RRB- cord-269867-k10siwur 71 33 ; ; : cord-269867-k10siwur 71 34 pVN1C pvn1c CD cord-269867-k10siwur 71 35 , , , cord-269867-k10siwur 71 36 pNP2C pnp2c CD cord-269867-k10siwur 71 37 , , , cord-269867-k10siwur 71 38 pPN3C pPN3C NNS cord-269867-k10siwur 71 39 , , , cord-269867-k10siwur 71 40 pNA4C pna4c CD cord-269867-k10siwur 71 41 , , , cord-269867-k10siwur 71 42 pAG5C pag5c CD cord-269867-k10siwur 71 43 , , , cord-269867-k10siwur 71 44 pGN6C pgn6c CD cord-269867-k10siwur 71 45 , , , cord-269867-k10siwur 71 46 pNR7C pnr7c XX cord-269867-k10siwur 71 47 and and CC cord-269867-k10siwur 71 48 pRG8C prg8c CD cord-269867-k10siwur 71 49 were be VBD cord-269867-k10siwur 71 50 obtained obtain VBN cord-269867-k10siwur 71 51 by by IN cord-269867-k10siwur 71 52 inserting insert VBG cord-269867-k10siwur 71 53 the the DT cord-269867-k10siwur 71 54 oligo oligo NN cord-269867-k10siwur 71 55 # # $ cord-269867-k10siwur 71 56 5 5 CD cord-269867-k10siwur 71 57 containing contain VBG cord-269867-k10siwur 71 58 XhoI XhoI NNP cord-269867-k10siwur 71 59 site site NN cord-269867-k10siwur 71 60 with with IN cord-269867-k10siwur 71 61 SpeI SpeI NNP cord-269867-k10siwur 71 62 compatible compatible JJ cord-269867-k10siwur 71 63 ends end VBZ cord-269867-k10siwur 71 64 into into IN cord-269867-k10siwur 71 65 the the DT cord-269867-k10siwur 71 66 SpeI SpeI NNP cord-269867-k10siwur 71 67 site site NN cord-269867-k10siwur 71 68 of of IN cord-269867-k10siwur 72 1 pVN1 pVN1 NNP cord-269867-k10siwur 72 2 , , , cord-269867-k10siwur 72 3 pNP2 pNP2 NNP cord-269867-k10siwur 72 4 , , , cord-269867-k10siwur 72 5 pPN3 pPN3 NNP cord-269867-k10siwur 72 6 , , , cord-269867-k10siwur 72 7 pNA4 pNA4 NNP cord-269867-k10siwur 72 8 , , , cord-269867-k10siwur 72 9 pAG5 pAG5 NNP cord-269867-k10siwur 72 10 , , , cord-269867-k10siwur 72 11 pGN6 pGN6 NNP cord-269867-k10siwur 72 12 , , , cord-269867-k10siwur 72 13 pNR7 pNR7 NNP cord-269867-k10siwur 72 14 and and CC cord-269867-k10siwur 72 15 pRG8 pRG8 NNP cord-269867-k10siwur 72 16 respectively respectively RB cord-269867-k10siwur 72 17 ; ; : cord-269867-k10siwur 72 18 pVN1AC pvn1ac UH cord-269867-k10siwur 72 19 , , , cord-269867-k10siwur 72 20 pPN3AC pPN3AC NNP cord-269867-k10siwur 72 21 , , , cord-269867-k10siwur 72 22 pNA4AC pna4ac LS cord-269867-k10siwur 72 23 , , , cord-269867-k10siwur 72 24 pGN6AC pGN6AC NNS cord-269867-k10siwur 72 25 and and CC cord-269867-k10siwur 72 26 pNR7AC pnr7ac JJ cord-269867-k10siwur 72 27 were be VBD cord-269867-k10siwur 72 28 made make VBN cord-269867-k10siwur 72 29 by by IN cord-269867-k10siwur 72 30 cloning clone VBG cord-269867-k10siwur 72 31 the the DT cord-269867-k10siwur 72 32 oligo oligo NN cord-269867-k10siwur 72 33 # # $ cord-269867-k10siwur 72 34 3 3 CD cord-269867-k10siwur 72 35 with with IN cord-269867-k10siwur 72 36 XhoI XhoI NNP cord-269867-k10siwur 72 37 - - HYPH cord-269867-k10siwur 72 38 compatible compatible JJ cord-269867-k10siwur 72 39 ends end NNS cord-269867-k10siwur 72 40 into into IN cord-269867-k10siwur 72 41 the the DT cord-269867-k10siwur 72 42 XhoI XhoI NNP cord-269867-k10siwur 72 43 site site NN cord-269867-k10siwur 72 44 of of IN cord-269867-k10siwur 72 45 pVN1C pvn1c CD cord-269867-k10siwur 72 46 , , , cord-269867-k10siwur 72 47 pPN3C pPN3C NNS cord-269867-k10siwur 72 48 , , , cord-269867-k10siwur 72 49 pNA4C pNA4C NNS cord-269867-k10siwur 72 50 , , , cord-269867-k10siwur 72 51 pGN6C pgn6c NN cord-269867-k10siwur 72 52 and and CC cord-269867-k10siwur 72 53 pNR7C pnr7c XX cord-269867-k10siwur 72 54 , , , cord-269867-k10siwur 72 55 respectively respectively RB cord-269867-k10siwur 72 56 ; ; : cord-269867-k10siwur 72 57 pVN1XC pvn1xc ADD cord-269867-k10siwur 72 58 , , , cord-269867-k10siwur 72 59 pNP2XC pnp2xc JJ cord-269867-k10siwur 72 60 , , , cord-269867-k10siwur 72 61 pPN3XC ppn3xc JJ cord-269867-k10siwur 72 62 , , , cord-269867-k10siwur 72 63 pAG5XC pag5xc NN cord-269867-k10siwur 72 64 and and CC cord-269867-k10siwur 72 65 pGN6XC pGN6XC NNS cord-269867-k10siwur 72 66 were be VBD cord-269867-k10siwur 72 67 engineered engineer VBN cord-269867-k10siwur 72 68 from from IN cord-269867-k10siwur 72 69 pVN1C pvn1c CD cord-269867-k10siwur 72 70 , , , cord-269867-k10siwur 72 71 pNP2C pnp2c CD cord-269867-k10siwur 72 72 , , , cord-269867-k10siwur 72 73 pPN3C ppn3c CD cord-269867-k10siwur 72 74 , , , cord-269867-k10siwur 72 75 pAG5C pag5c CD cord-269867-k10siwur 72 76 and and CC cord-269867-k10siwur 72 77 pGN6C pgn6c NN cord-269867-k10siwur 72 78 , , , cord-269867-k10siwur 72 79 respectively respectively RB cord-269867-k10siwur 72 80 , , , cord-269867-k10siwur 72 81 as as IN cord-269867-k10siwur 72 82 indicated indicate VBN cord-269867-k10siwur 72 83 just just RB cord-269867-k10siwur 72 84 above above RB cord-269867-k10siwur 72 85 except except IN cord-269867-k10siwur 72 86 that that DT cord-269867-k10siwur 72 87 oligo oligo NN cord-269867-k10siwur 72 88 # # NN cord-269867-k10siwur 72 89 3 3 CD cord-269867-k10siwur 72 90 was be VBD cord-269867-k10siwur 72 91 inserted insert VBN cord-269867-k10siwur 72 92 in in IN cord-269867-k10siwur 72 93 - - HYPH cord-269867-k10siwur 72 94 frame frame NN cord-269867-k10siwur 72 95 in in IN cord-269867-k10siwur 72 96 the the DT cord-269867-k10siwur 72 97 reverse reverse JJ cord-269867-k10siwur 72 98 orientation orientation NN cord-269867-k10siwur 72 99 , , , cord-269867-k10siwur 72 100 as as IN cord-269867-k10siwur 72 101 designated designate VBN cord-269867-k10siwur 72 102 by by IN cord-269867-k10siwur 72 103 the the DT cord-269867-k10siwur 72 104 letter letter NN cord-269867-k10siwur 72 105 X x NN cord-269867-k10siwur 72 106 ; ; : cord-269867-k10siwur 72 107 pDEV2CA pDEV2CA NNP cord-269867-k10siwur 72 108 was be VBD cord-269867-k10siwur 72 109 made make VBN cord-269867-k10siwur 72 110 by by IN cord-269867-k10siwur 72 111 replacing replace VBG cord-269867-k10siwur 72 112 a a DT cord-269867-k10siwur 72 113 0.3-kb 0.3-kb CD cord-269867-k10siwur 72 114 MunI MunI NNP cord-269867-k10siwur 72 115 - - HYPH cord-269867-k10siwur 72 116 SacI SacI NNP cord-269867-k10siwur 72 117 fragment fragment NN cord-269867-k10siwur 72 118 from from IN cord-269867-k10siwur 72 119 pGCA41155 pGCA41155 NNS cord-269867-k10siwur 72 120 with with IN cord-269867-k10siwur 72 121 the the DT cord-269867-k10siwur 72 122 0.32-kb 0.32-kb CD cord-269867-k10siwur 72 123 MunI MunI NNP cord-269867-k10siwur 72 124 - - HYPH cord-269867-k10siwur 72 125 SacI SacI NNP cord-269867-k10siwur 72 126 fragment fragment NN cord-269867-k10siwur 72 127 from from IN cord-269867-k10siwur 72 128 pGCAI02 pgcai02 NN cord-269867-k10siwur 72 129 . . . cord-269867-k10siwur 73 1 ( ( -LRB- cord-269867-k10siwur 73 2 c c NN cord-269867-k10siwur 73 3 ) ) -RRB- cord-269867-k10siwur 74 1 The the DT cord-269867-k10siwur 74 2 presence presence NN cord-269867-k10siwur 74 3 olTGEV oltgev NN cord-269867-k10siwur 74 4 - - HYPH cord-269867-k10siwur 74 5 C c NN cord-269867-k10siwur 74 6 and and CC cord-269867-k10siwur 74 7 -A -A NNS cord-269867-k10siwur 74 8 is be VBZ cord-269867-k10siwur 74 9 specified specify VBN cord-269867-k10siwur 74 10 by by IN cord-269867-k10siwur 74 11 the the DT cord-269867-k10siwur 74 12 letters letter NNS cord-269867-k10siwur 74 13 C c NN cord-269867-k10siwur 74 14 and and CC cord-269867-k10siwur 74 15 A a DT cord-269867-k10siwur 74 16 respectively respectively RB cord-269867-k10siwur 74 17 ; ; : cord-269867-k10siwur 74 18 CA CA NNP cord-269867-k10siwur 74 19 or or CC cord-269867-k10siwur 74 20 AC AC NNP cord-269867-k10siwur 74 21 , , , cord-269867-k10siwur 74 22 ( ( -LRB- cord-269867-k10siwur 74 23 Fig fig NN cord-269867-k10siwur 74 24 . . NNP cord-269867-k10siwur 74 25 2 2 CD cord-269867-k10siwur 74 26 ) ) -RRB- cord-269867-k10siwur 74 27 . . . cord-269867-k10siwur 75 1 All all PDT cord-269867-k10siwur 75 2 these these DT cord-269867-k10siwur 75 3 constructions construction NNS cord-269867-k10siwur 75 4 summarized summarize VBN cord-269867-k10siwur 75 5 in in IN cord-269867-k10siwur 75 6 Fig Fig NNP cord-269867-k10siwur 75 7 . . . cord-269867-k10siwur 76 1 3 3 CD cord-269867-k10siwur 76 2 are be VBP cord-269867-k10siwur 76 3 described describe VBN cord-269867-k10siwur 76 4 in in IN cord-269867-k10siwur 76 5 detail detail NN cord-269867-k10siwur 76 6 in in IN cord-269867-k10siwur 76 7 the the DT cord-269867-k10siwur 76 8 Fig Fig NNP cord-269867-k10siwur 76 9 . . . cord-269867-k10siwur 77 1 3 3 CD cord-269867-k10siwur 77 2 legend legend NN cord-269867-k10siwur 77 3 . . . cord-269867-k10siwur 78 1 In in IN cord-269867-k10siwur 78 2 total total JJ cord-269867-k10siwur 78 3 , , , cord-269867-k10siwur 78 4 36 36 CD cord-269867-k10siwur 78 5 mutants mutant NNS cord-269867-k10siwur 78 6 were be VBD cord-269867-k10siwur 78 7 obtained obtain VBN cord-269867-k10siwur 78 8 . . . cord-269867-k10siwur 79 1 Twenty twenty CD cord-269867-k10siwur 79 2 - - HYPH cord-269867-k10siwur 79 3 eight eight CD cord-269867-k10siwur 79 4 of of IN cord-269867-k10siwur 79 5 them -PRON- PRP cord-269867-k10siwur 79 6 contained contain VBD cord-269867-k10siwur 79 7 at at IN cord-269867-k10siwur 79 8 least least JJS cord-269867-k10siwur 79 9 one one CD cord-269867-k10siwur 79 10 TGEV TGEV NNP cord-269867-k10siwur 79 11 peptide peptide NN cord-269867-k10siwur 79 12 , , , cord-269867-k10siwur 79 13 among among IN cord-269867-k10siwur 79 14 which which WDT cord-269867-k10siwur 79 15 10 10 CD cord-269867-k10siwur 79 16 had have VBD cord-269867-k10siwur 79 17 both both DT cord-269867-k10siwur 79 18 TGEV TGEV NNP cord-269867-k10siwur 79 19 - - HYPH cord-269867-k10siwur 79 20 C C NNP cord-269867-k10siwur 79 21 and and CC cord-269867-k10siwur 79 22 -A -A : cord-269867-k10siwur 79 23 as as IN cord-269867-k10siwur 79 24 an an DT cord-269867-k10siwur 79 25 A::C a::c JJ cord-269867-k10siwur 79 26 or or CC cord-269867-k10siwur 79 27 C::A c::a JJ cord-269867-k10siwur 79 28 fusion fusion NN cord-269867-k10siwur 79 29 . . . cord-269867-k10siwur 80 1 Finally finally RB cord-269867-k10siwur 80 2 , , , cord-269867-k10siwur 80 3 modifications modification NNS cord-269867-k10siwur 80 4 in in IN cord-269867-k10siwur 80 5 ClpG ClpG NNP cord-269867-k10siwur 80 6 resulted result VBD cord-269867-k10siwur 80 7 in in IN cord-269867-k10siwur 80 8 an an DT cord-269867-k10siwur 80 9 insert insert NN cord-269867-k10siwur 80 10 of of IN cord-269867-k10siwur 80 11 2 2 CD cord-269867-k10siwur 80 12 , , , cord-269867-k10siwur 80 13 13 13 CD cord-269867-k10siwur 80 14 , , , cord-269867-k10siwur 80 15 14 14 CD cord-269867-k10siwur 80 16 , , , cord-269867-k10siwur 80 17 25 25 CD cord-269867-k10siwur 80 18 , , , cord-269867-k10siwur 80 19 26 26 CD cord-269867-k10siwur 80 20 or or CC cord-269867-k10siwur 80 21 51 51 CD cord-269867-k10siwur 80 22 aa aa NN cord-269867-k10siwur 80 23 in in IN cord-269867-k10siwur 80 24 length length NN cord-269867-k10siwur 80 25 ( ( -LRB- cord-269867-k10siwur 80 26 Fig fig NN cord-269867-k10siwur 80 27 . . . cord-269867-k10siwur 80 28 3 3 CD cord-269867-k10siwur 80 29 ) ) -RRB- cord-269867-k10siwur 80 30 . . . cord-269867-k10siwur 81 1 Each each DT cord-269867-k10siwur 81 2 of of IN cord-269867-k10siwur 81 3 the the DT cord-269867-k10siwur 81 4 36 36 CD cord-269867-k10siwur 81 5 mutant mutant JJ cord-269867-k10siwur 81 6 plasmids plasmid NNS cord-269867-k10siwur 81 7 ( ( -LRB- cord-269867-k10siwur 81 8 Fig Fig NNP cord-269867-k10siwur 81 9 . . . cord-269867-k10siwur 81 10 3 3 LS cord-269867-k10siwur 81 11 ) ) -RRB- cord-269867-k10siwur 81 12 was be VBD cord-269867-k10siwur 81 13 transferred transfer VBN cord-269867-k10siwur 81 14 into into IN cord-269867-k10siwur 81 15 E. E. NNP cord-269867-k10siwur 81 16 coli coli NNS cord-269867-k10siwur 82 1 DH5~ DH5~ NNS cord-269867-k10siwur 82 2 bearing bear VBG cord-269867-k10siwur 82 3 the the DT cord-269867-k10siwur 82 4 trans trans NN cord-269867-k10siwur 82 5 - - JJ cord-269867-k10siwur 82 6 complementing complement VBG cord-269867-k10siwur 82 7 plasmid plasmid NN cord-269867-k10siwur 82 8 pDSPH524 pDSPH524 NNP cord-269867-k10siwur 82 9 . . . cord-269867-k10siwur 83 1 The the DT cord-269867-k10siwur 83 2 cell cell NN cord-269867-k10siwur 83 3 - - HYPH cord-269867-k10siwur 83 4 surface surface NN cord-269867-k10siwur 83 5 location location NN cord-269867-k10siwur 83 6 of of IN cord-269867-k10siwur 83 7 the the DT cord-269867-k10siwur 83 8 corresponding corresponding JJ cord-269867-k10siwur 83 9 hybrid hybrid NN cord-269867-k10siwur 83 10 ClpG ClpG NNP cord-269867-k10siwur 83 11 subunits subunit NNS cord-269867-k10siwur 83 12 ( ( -LRB- cord-269867-k10siwur 83 13 Fig Fig NNP cord-269867-k10siwur 83 14 . . . cord-269867-k10siwur 83 15 3 3 CD cord-269867-k10siwur 83 16 ) ) -RRB- cord-269867-k10siwur 84 1 on on IN cord-269867-k10siwur 84 2 CS31A cs31a NN cord-269867-k10siwur 85 1 fibrillae fibrillae NNP cord-269867-k10siwur 85 2 was be VBD cord-269867-k10siwur 85 3 determined determine VBN cord-269867-k10siwur 85 4 on on IN cord-269867-k10siwur 85 5 intact intact JJ cord-269867-k10siwur 85 6 cells cell NNS cord-269867-k10siwur 85 7 by by IN cord-269867-k10siwur 85 8 in in IN cord-269867-k10siwur 85 9 situ situ NN cord-269867-k10siwur 85 10 colony colony NN cord-269867-k10siwur 85 11 immunoblotting immunoblotting NN cord-269867-k10siwur 85 12 and and CC cord-269867-k10siwur 85 13 from from IN cord-269867-k10siwur 85 14 isolated isolated JJ cord-269867-k10siwur 85 15 mutated mutated JJ cord-269867-k10siwur 85 16 CS31A cs31a NN cord-269867-k10siwur 85 17 polymers polymer NNS cord-269867-k10siwur 85 18 by by IN cord-269867-k10siwur 85 19 immunodot immunodot NNP cord-269867-k10siwur 85 20 analysis analysis NN cord-269867-k10siwur 85 21 using use VBG cord-269867-k10siwur 85 22 a a DT cord-269867-k10siwur 85 23 CS31A cs31a NN cord-269867-k10siwur 85 24 - - HYPH cord-269867-k10siwur 85 25 specific specific JJ cord-269867-k10siwur 85 26 polyclonal polyclonal JJ cord-269867-k10siwur 85 27 Ab ab NN cord-269867-k10siwur 85 28 ( ( -LRB- cord-269867-k10siwur 85 29 pAb pAb NNP cord-269867-k10siwur 85 30 ) ) -RRB- cord-269867-k10siwur 85 31 , , , cord-269867-k10siwur 85 32 the the DT cord-269867-k10siwur 85 33 TGEV TGEV NNP cord-269867-k10siwur 85 34 - - HYPH cord-269867-k10siwur 85 35 C C NNP cord-269867-k10siwur 85 36 - - HYPH cord-269867-k10siwur 85 37 specific specific JJ cord-269867-k10siwur 85 38 3b.5 3b.5 CD cord-269867-k10siwur 85 39 mAb mAb NNS cord-269867-k10siwur 85 40 and and CC cord-269867-k10siwur 85 41 the the DT cord-269867-k10siwur 85 42 TGEV TGEV NNP cord-269867-k10siwur 85 43 - - HYPH cord-269867-k10siwur 85 44 A a NN cord-269867-k10siwur 85 45 - - HYPH cord-269867-k10siwur 85 46 specific specific JJ cord-269867-k10siwur 85 47 1AF10 1af10 CD cord-269867-k10siwur 85 48 mAb mAb NNS cord-269867-k10siwur 85 49 ( ( -LRB- cord-269867-k10siwur 85 50 not not RB cord-269867-k10siwur 85 51 shown show VBN cord-269867-k10siwur 85 52 ) ) -RRB- cord-269867-k10siwur 85 53 . . . cord-269867-k10siwur 86 1 Out out IN cord-269867-k10siwur 86 2 of of IN cord-269867-k10siwur 86 3 28 28 CD cord-269867-k10siwur 86 4 recombinant recombinant JJ cord-269867-k10siwur 86 5 CS31A cs31a NN cord-269867-k10siwur 86 6 fibrillae fibrillae NNP cord-269867-k10siwur 86 7 carrying carry VBG cord-269867-k10siwur 86 8 at at RB cord-269867-k10siwur 86 9 least least RBS cord-269867-k10siwur 86 10 one one CD cord-269867-k10siwur 86 11 TGEV TGEV NNP cord-269867-k10siwur 86 12 peptide peptide NN cord-269867-k10siwur 86 13 only only RB cord-269867-k10siwur 86 14 13 13 CD cord-269867-k10siwur 86 15 failed fail VBD cord-269867-k10siwur 86 16 to to TO cord-269867-k10siwur 86 17 react react VB cord-269867-k10siwur 86 18 whatever whatever WDT cord-269867-k10siwur 86 19 Ab ab NN cord-269867-k10siwur 86 20 . . . cord-269867-k10siwur 87 1 In in IN cord-269867-k10siwur 87 2 contrast contrast NN cord-269867-k10siwur 87 3 , , , cord-269867-k10siwur 87 4 the the DT cord-269867-k10siwur 87 5 15 15 CD cord-269867-k10siwur 87 6 remaining remain VBG cord-269867-k10siwur 87 7 hybrids hybrid NNS cord-269867-k10siwur 87 8 were be VBD cord-269867-k10siwur 87 9 capable capable JJ cord-269867-k10siwur 87 10 of of IN cord-269867-k10siwur 87 11 exposing expose VBG cord-269867-k10siwur 87 12 the the DT cord-269867-k10siwur 87 13 TGEV TGEV NNP cord-269867-k10siwur 87 14 peptides peptide NNS cord-269867-k10siwur 87 15 at at IN cord-269867-k10siwur 87 16 the the DT cord-269867-k10siwur 87 17 cell cell NN cord-269867-k10siwur 87 18 surface surface NN cord-269867-k10siwur 87 19 on on IN cord-269867-k10siwur 87 20 the the DT cord-269867-k10siwur 87 21 correctly correctly RB cord-269867-k10siwur 87 22 assembled assemble VBN cord-269867-k10siwur 87 23 CS31A CS31A NNP cord-269867-k10siwur 87 24 fibrillae fibrillae NNP cord-269867-k10siwur 87 25 . . . cord-269867-k10siwur 88 1 Nine nine CD cord-269867-k10siwur 88 2 permissive permissive JJ cord-269867-k10siwur 88 3 were be VBD cord-269867-k10siwur 88 4 identified identify VBN cord-269867-k10siwur 88 5 throughout throughout IN cord-269867-k10siwur 88 6 ClpG. ClpG. . cord-269867-k10siwur 89 1 The the DT cord-269867-k10siwur 89 2 positions position NNS cord-269867-k10siwur 89 3 aa aa NNP cord-269867-k10siwur 89 4 -1/1 -1/1 NNP cord-269867-k10siwur 89 5 , , , cord-269867-k10siwur 89 6 202 202 CD cord-269867-k10siwur 89 7 - - SYM cord-269867-k10siwur 89 8 204 204 CD cord-269867-k10siwur 89 9 and and CC cord-269867-k10siwur 89 10 202 202 CD cord-269867-k10siwur 89 11 - - SYM cord-269867-k10siwur 89 12 218 218 CD cord-269867-k10siwur 89 13 appeared appear VBD cord-269867-k10siwur 89 14 to to TO cord-269867-k10siwur 89 15 be be VB cord-269867-k10siwur 89 16 the the DT cord-269867-k10siwur 89 17 most most RBS cord-269867-k10siwur 89 18 permissive permissive JJ cord-269867-k10siwur 89 19 targets target NNS cord-269867-k10siwur 89 20 since since IN cord-269867-k10siwur 89 21 the the DT cord-269867-k10siwur 89 22 largest large JJS cord-269867-k10siwur 89 23 insertions insertion NNS cord-269867-k10siwur 89 24 ( ( -LRB- cord-269867-k10siwur 89 25 25 25 CD cord-269867-k10siwur 89 26 or or CC cord-269867-k10siwur 89 27 26 26 CD cord-269867-k10siwur 89 28 aa aa NNP cord-269867-k10siwur 89 29 ) ) -RRB- cord-269867-k10siwur 89 30 , , , cord-269867-k10siwur 89 31 did do VBD cord-269867-k10siwur 89 32 not not RB cord-269867-k10siwur 89 33 interfere interfere VB cord-269867-k10siwur 89 34 with with IN cord-269867-k10siwur 89 35 the the DT cord-269867-k10siwur 89 36 CS31A CS31A NNP cord-269867-k10siwur 89 37 fibrillae fibrillae NNP cord-269867-k10siwur 89 38 formation formation NN cord-269867-k10siwur 89 39 . . . cord-269867-k10siwur 90 1 Even even RB cord-269867-k10siwur 90 2 the the DT cord-269867-k10siwur 90 3 hybrid hybrid JJ cord-269867-k10siwur 90 4 ClpG1/203-CA ClpG1/203-CA NNP cord-269867-k10siwur 90 5 protein protein NN cord-269867-k10siwur 90 6 with with IN cord-269867-k10siwur 90 7 an an DT cord-269867-k10siwur 90 8 insert insert NN cord-269867-k10siwur 90 9 of of IN cord-269867-k10siwur 90 10 51 51 CD cord-269867-k10siwur 90 11 aa aa NNP cord-269867-k10siwur 90 12 long long RB cord-269867-k10siwur 90 13 , , , cord-269867-k10siwur 90 14 resulting result VBG cord-269867-k10siwur 90 15 from from IN cord-269867-k10siwur 90 16 the the DT cord-269867-k10siwur 90 17 simultaneous simultaneous JJ cord-269867-k10siwur 90 18 tandem tandem NN cord-269867-k10siwur 90 19 addition addition NN cord-269867-k10siwur 90 20 of of IN cord-269867-k10siwur 90 21 the the DT cord-269867-k10siwur 90 22 two two CD cord-269867-k10siwur 90 23 TGEV TGEV NNP cord-269867-k10siwur 90 24 peptides peptide NNS cord-269867-k10siwur 90 25 in in IN cord-269867-k10siwur 90 26 both both DT cord-269867-k10siwur 90 27 positions position NNS cord-269867-k10siwur 91 1 aa aa NNP cord-269867-k10siwur 91 2 -1/1 -1/1 NNP cord-269867-k10siwur 91 3 and and CC cord-269867-k10siwur 91 4 202 202 CD cord-269867-k10siwur 91 5 - - SYM cord-269867-k10siwur 91 6 218 218 CD cord-269867-k10siwur 91 7 , , , cord-269867-k10siwur 91 8 was be VBD cord-269867-k10siwur 91 9 incorporated incorporate VBN cord-269867-k10siwur 91 10 in in IN cord-269867-k10siwur 91 11 CS31A cs31a NN cord-269867-k10siwur 91 12 polymer polymer NN cord-269867-k10siwur 91 13 . . . cord-269867-k10siwur 92 1 In in IN cord-269867-k10siwur 92 2 positions position NNS cord-269867-k10siwur 92 3 aa aa NNP cord-269867-k10siwur 92 4 192 192 CD cord-269867-k10siwur 92 5 to to IN cord-269867-k10siwur 92 6 197 197 CD cord-269867-k10siwur 92 7 less less JJR cord-269867-k10siwur 92 8 residues residue NNS cord-269867-k10siwur 92 9 ( ( -LRB- cord-269867-k10siwur 92 10 14 14 CD cord-269867-k10siwur 92 11 aa aa NNP cord-269867-k10siwur 92 12 ) ) -RRB- cord-269867-k10siwur 92 13 was be VBD cord-269867-k10siwur 92 14 tolerated tolerate VBN cord-269867-k10siwur 92 15 . . . cord-269867-k10siwur 93 1 Positions position NNS cord-269867-k10siwur 93 2 aa aa NNP cord-269867-k10siwur 93 3 182 182 CD cord-269867-k10siwur 93 4 - - SYM cord-269867-k10siwur 93 5 183 183 CD cord-269867-k10siwur 93 6 , , , cord-269867-k10siwur 93 7 190 190 CD cord-269867-k10siwur 93 8 - - SYM cord-269867-k10siwur 93 9 191 191 CD cord-269867-k10siwur 93 10 and and CC cord-269867-k10siwur 93 11 191 191 CD cord-269867-k10siwur 93 12 - - SYM cord-269867-k10siwur 93 13 192 192 CD cord-269867-k10siwur 93 14 were be VBD cord-269867-k10siwur 93 15 nonpermissive nonpermissive JJ cord-269867-k10siwur 93 16 since since IN cord-269867-k10siwur 93 17 , , , cord-269867-k10siwur 93 18 except except IN cord-269867-k10siwur 93 19 for for IN cord-269867-k10siwur 93 20 ClpG190 ClpG190 NNP cord-269867-k10siwur 93 21 with with IN cord-269867-k10siwur 93 22 only only RB cord-269867-k10siwur 93 23 two two CD cord-269867-k10siwur 93 24 extra extra JJ cord-269867-k10siwur 93 25 aa aa NN cord-269867-k10siwur 93 26 , , , cord-269867-k10siwur 93 27 no no DT cord-269867-k10siwur 93 28 hybrid hybrid NN cord-269867-k10siwur 93 29 was be VBD cord-269867-k10siwur 93 30 detected detect VBN cord-269867-k10siwur 93 31 . . . cord-269867-k10siwur 94 1 All all DT cord-269867-k10siwur 94 2 nonpermissible nonpermissible JJ cord-269867-k10siwur 94 3 insertions insertion NNS cord-269867-k10siwur 94 4 were be VBD cord-269867-k10siwur 94 5 located locate VBN cord-269867-k10siwur 94 6 in in IN cord-269867-k10siwur 94 7 or or CC cord-269867-k10siwur 94 8 near near IN cord-269867-k10siwur 94 9 a a DT cord-269867-k10siwur 94 10 predicted predict VBN cord-269867-k10siwur 94 11 a a DT cord-269867-k10siwur 94 12 - - HYPH cord-269867-k10siwur 94 13 helix helix NN cord-269867-k10siwur 94 14 structure structure NN cord-269867-k10siwur 94 15 , , , cord-269867-k10siwur 94 16 and and CC cord-269867-k10siwur 94 17 targeted target VBD cord-269867-k10siwur 94 18 conserved conserve VBN cord-269867-k10siwur 94 19 aa aa NN cord-269867-k10siwur 94 20 residues residue NNS cord-269867-k10siwur 94 21 ( ( -LRB- cord-269867-k10siwur 94 22 Fig Fig NNP cord-269867-k10siwur 94 23 . . NNP cord-269867-k10siwur 94 24 1 1 CD cord-269867-k10siwur 94 25 , , , cord-269867-k10siwur 94 26 aa aa NNP cord-269867-k10siwur 94 27 182 182 CD cord-269867-k10siwur 94 28 -193 -193 NN cord-269867-k10siwur 94 29 ) ) -RRB- cord-269867-k10siwur 94 30 . . . cord-269867-k10siwur 95 1 By by IN cord-269867-k10siwur 95 2 contrast contrast NN cord-269867-k10siwur 95 3 , , , cord-269867-k10siwur 95 4 all all DT cord-269867-k10siwur 95 5 permissive permissive JJ cord-269867-k10siwur 95 6 sites site NNS cord-269867-k10siwur 95 7 , , , cord-269867-k10siwur 95 8 excluding exclude VBG cord-269867-k10siwur 95 9 the the DT cord-269867-k10siwur 95 10 ClpG ClpG NNP cord-269867-k10siwur 95 11 - - HYPH cord-269867-k10siwur 95 12 N N NNP cord-269867-k10siwur 95 13 terminus terminus NN cord-269867-k10siwur 95 14 , , , cord-269867-k10siwur 95 15 were be VBD cord-269867-k10siwur 95 16 included include VBN cord-269867-k10siwur 95 17 in in IN cord-269867-k10siwur 95 18 a a DT cord-269867-k10siwur 95 19 predicted predict VBN cord-269867-k10siwur 95 20 loop loop NN cord-269867-k10siwur 95 21 ( ( -LRB- cord-269867-k10siwur 95 22 Fig Fig NNP cord-269867-k10siwur 95 23 . . NNP cord-269867-k10siwur 95 24 1 1 CD cord-269867-k10siwur 95 25 , , , cord-269867-k10siwur 95 26 aa aa NNP cord-269867-k10siwur 95 27 194 194 CD cord-269867-k10siwur 95 28 - - HYPH cord-269867-k10siwur 95 29 212 212 CD cord-269867-k10siwur 95 30 ) ) -RRB- cord-269867-k10siwur 95 31 that that WDT cord-269867-k10siwur 95 32 is be VBZ cord-269867-k10siwur 95 33 more more RBR cord-269867-k10siwur 95 34 likely likely JJ cord-269867-k10siwur 95 35 to to TO cord-269867-k10siwur 95 36 be be VB cord-269867-k10siwur 95 37 flexible flexible JJ cord-269867-k10siwur 95 38 enough enough RB cord-269867-k10siwur 95 39 to to TO cord-269867-k10siwur 95 40 accommodate accommodate VB cord-269867-k10siwur 95 41 large large JJ cord-269867-k10siwur 95 42 inserts insert NNS cord-269867-k10siwur 95 43 , , , cord-269867-k10siwur 95 44 as as IN cord-269867-k10siwur 95 45 indicated indicate VBN cord-269867-k10siwur 95 46 by by IN cord-269867-k10siwur 95 47 the the DT cord-269867-k10siwur 95 48 proposed propose VBN cord-269867-k10siwur 95 49 CIpG CIpG NNP cord-269867-k10siwur 95 50 topology topology NN cord-269867-k10siwur 95 51 ( ( -LRB- cord-269867-k10siwur 95 52 Mrchin Mrchin NNP cord-269867-k10siwur 95 53 et et NNP cord-269867-k10siwur 95 54 al al NNP cord-269867-k10siwur 95 55 . . NNP cord-269867-k10siwur 95 56 , , , cord-269867-k10siwur 95 57 1995 1995 CD cord-269867-k10siwur 95 58 ) ) -RRB- cord-269867-k10siwur 95 59 . . . cord-269867-k10siwur 96 1 While while IN cord-269867-k10siwur 96 2 the the DT cord-269867-k10siwur 96 3 two two CD cord-269867-k10siwur 96 4 fully fully RB cord-269867-k10siwur 96 5 permissive permissive JJ cord-269867-k10siwur 96 6 sites site NNS cord-269867-k10siwur 96 7 ( ( -LRB- cord-269867-k10siwur 96 8 aa aa NNP cord-269867-k10siwur 96 9 202 202 CD cord-269867-k10siwur 96 10 - - HYPH cord-269867-k10siwur 96 11 204 204 CD cord-269867-k10siwur 96 12 and and CC cord-269867-k10siwur 96 13 202 202 CD cord-269867-k10siwur 96 14 - - SYM cord-269867-k10siwur 96 15 218 218 CD cord-269867-k10siwur 96 16 ) ) -RRB- cord-269867-k10siwur 96 17 were be VBD cord-269867-k10siwur 96 18 located locate VBN cord-269867-k10siwur 96 19 between between IN cord-269867-k10siwur 96 20 the the DT cord-269867-k10siwur 96 21 top top NN cord-269867-k10siwur 96 22 and and CC cord-269867-k10siwur 96 23 the the DT cord-269867-k10siwur 96 24 end end NN cord-269867-k10siwur 96 25 of of IN cord-269867-k10siwur 96 26 the the DT cord-269867-k10siwur 96 27 loop loop NN cord-269867-k10siwur 96 28 , , , cord-269867-k10siwur 96 29 the the DT cord-269867-k10siwur 96 30 six six CD cord-269867-k10siwur 96 31 partially partially RB cord-269867-k10siwur 96 32 permissive permissive JJ cord-269867-k10siwur 96 33 sites site NNS cord-269867-k10siwur 96 34 ( ( -LRB- cord-269867-k10siwur 96 35 aa aa NNP cord-269867-k10siwur 96 36 192 192 CD cord-269867-k10siwur 96 37 to to IN cord-269867-k10siwur 96 38 197 197 CD cord-269867-k10siwur 96 39 ) ) -RRB- cord-269867-k10siwur 96 40 targeted target VBN cord-269867-k10siwur 96 41 region region NN cord-269867-k10siwur 96 42 immediately immediately RB cord-269867-k10siwur 96 43 beginning begin VBG cord-269867-k10siwur 96 44 this this DT cord-269867-k10siwur 96 45 loop loop NN cord-269867-k10siwur 96 46 . . . cord-269867-k10siwur 97 1 These these DT cord-269867-k10siwur 97 2 data datum NNS cord-269867-k10siwur 97 3 suggest suggest VBP cord-269867-k10siwur 97 4 a a DT cord-269867-k10siwur 97 5 relationship relationship NN cord-269867-k10siwur 97 6 between between IN cord-269867-k10siwur 97 7 the the DT cord-269867-k10siwur 97 8 permissivity permissivity NN cord-269867-k10siwur 97 9 of of IN cord-269867-k10siwur 97 10 ClpG ClpG NNP cord-269867-k10siwur 97 11 and and CC cord-269867-k10siwur 97 12 the the DT cord-269867-k10siwur 97 13 local local JJ cord-269867-k10siwur 97 14 CIpG CIpG NNP cord-269867-k10siwur 97 15 structures structure NNS cord-269867-k10siwur 97 16 into into IN cord-269867-k10siwur 97 17 which which WDT cord-269867-k10siwur 97 18 the the DT cord-269867-k10siwur 97 19 epitope epitope NN cord-269867-k10siwur 97 20 was be VBD cord-269867-k10siwur 97 21 inserted insert VBN cord-269867-k10siwur 97 22 . . . cord-269867-k10siwur 98 1 On on IN cord-269867-k10siwur 98 2 the the DT cord-269867-k10siwur 98 3 basis basis NN cord-269867-k10siwur 98 4 of of IN cord-269867-k10siwur 98 5 these these DT cord-269867-k10siwur 98 6 results result NNS cord-269867-k10siwur 98 7 , , , cord-269867-k10siwur 98 8 we -PRON- PRP cord-269867-k10siwur 98 9 conclude conclude VBP cord-269867-k10siwur 98 10 that that IN cord-269867-k10siwur 98 11 ClpG ClpG NNP cord-269867-k10siwur 98 12 as as IN cord-269867-k10siwur 98 13 a a DT cord-269867-k10siwur 98 14 carrier carrier NN cord-269867-k10siwur 98 15 is be VBZ cord-269867-k10siwur 98 16 very very RB cord-269867-k10siwur 98 17 flexible flexible JJ cord-269867-k10siwur 98 18 since since IN cord-269867-k10siwur 98 19 insertions insertion NNS cord-269867-k10siwur 98 20 varying vary VBG cord-269867-k10siwur 98 21 in in IN cord-269867-k10siwur 98 22 length length NN cord-269867-k10siwur 98 23 and and CC cord-269867-k10siwur 98 24 nature nature NN cord-269867-k10siwur 98 25 can can MD cord-269867-k10siwur 98 26 be be VB cord-269867-k10siwur 98 27 made make VBN cord-269867-k10siwur 98 28 in in IN cord-269867-k10siwur 98 29 different different JJ cord-269867-k10siwur 98 30 sites site NNS cord-269867-k10siwur 98 31 without without IN cord-269867-k10siwur 98 32 affecting affect VBG cord-269867-k10siwur 98 33 CS31A cs31a NN cord-269867-k10siwur 98 34 formation formation NN cord-269867-k10siwur 98 35 . . . cord-269867-k10siwur 99 1 Only only RB cord-269867-k10siwur 99 2 hybrid hybrid JJ cord-269867-k10siwur 99 3 ClpG ClpG NNP cord-269867-k10siwur 99 4 subunits subunit NNS cord-269867-k10siwur 99 5 displaying display VBG cord-269867-k10siwur 99 6 at at IN cord-269867-k10siwur 99 7 least least JJS cord-269867-k10siwur 99 8 one one CD cord-269867-k10siwur 99 9 TGEV tgev NN cord-269867-k10siwur 99 10 peptide peptide NN cord-269867-k10siwur 99 11 on on IN cord-269867-k10siwur 99 12 CS31A cs31a NN cord-269867-k10siwur 99 13 were be VBD cord-269867-k10siwur 99 14 characterized characterize VBN cord-269867-k10siwur 99 15 by by IN cord-269867-k10siwur 99 16 Western western JJ cord-269867-k10siwur 99 17 immunoblot immunoblot NNS cord-269867-k10siwur 99 18 analysis analysis NN cord-269867-k10siwur 99 19 ( ( -LRB- cord-269867-k10siwur 99 20 Fig Fig NNP cord-269867-k10siwur 99 21 . . NNP cord-269867-k10siwur 99 22 4 4 CD cord-269867-k10siwur 99 23 ) ) -RRB- cord-269867-k10siwur 99 24 . . . cord-269867-k10siwur 100 1 Proteins protein NNS cord-269867-k10siwur 100 2 CIpG192-C CIpG192-C VBN cord-269867-k10siwur 100 3 , , , cord-269867-k10siwur 100 4 ClpG193-C clpg193-c NN cord-269867-k10siwur 100 5 , , , cord-269867-k10siwur 100 6 ClpG194-C ClpG194-C NNP cord-269867-k10siwur 100 7 , , , cord-269867-k10siwur 100 8 ClpG195-C clpg195-c NN cord-269867-k10siwur 100 9 , , , cord-269867-k10siwur 100 10 ClpG196-C ClpG196-C NNS cord-269867-k10siwur 100 11 , , , cord-269867-k10siwur 100 12 Clp Clp NNP cord-269867-k10siwur 100 13 G197-C G197-C NNP cord-269867-k10siwur 100 14 , , , cord-269867-k10siwur 100 15 ClpG202-XC ClpG202-XC NNPS cord-269867-k10siwur 100 16 , , , cord-269867-k10siwur 100 17 ClpG1-CA ClpG1-CA NNP cord-269867-k10siwur 100 18 , , , cord-269867-k10siwur 101 1 ClpG1-C ClpG1-C NNP cord-269867-k10siwur 101 2 , , , cord-269867-k10siwur 101 3 GlpG1-AC GlpG1-AC NNP cord-269867-k10siwur 101 4 and and CC cord-269867-k10siwur 101 5 ClpG202-C ClpG202-C NNP cord-269867-k10siwur 101 6 migrated migrate VBN cord-269867-k10siwur 101 7 as as IN cord-269867-k10siwur 101 8 a a DT cord-269867-k10siwur 101 9 single single JJ cord-269867-k10siwur 101 10 band band NN cord-269867-k10siwur 101 11 which which WDT cord-269867-k10siwur 101 12 corresponds correspond VBZ cord-269867-k10siwur 101 13 to to IN cord-269867-k10siwur 101 14 the the DT cord-269867-k10siwur 101 15 expected expect VBN cord-269867-k10siwur 101 16 full full JJ cord-269867-k10siwur 101 17 - - HYPH cord-269867-k10siwur 101 18 length length NN cord-269867-k10siwur 101 19 hybrid hybrid NN cord-269867-k10siwur 101 20 since since IN cord-269867-k10siwur 101 21 revealed reveal VBN cord-269867-k10siwur 101 22 by by IN cord-269867-k10siwur 101 23 anti anti JJ cord-269867-k10siwur 101 24 - - JJ cord-269867-k10siwur 101 25 ClpG ClpG NNP cord-269867-k10siwur 101 26 pAb pab NN cord-269867-k10siwur 101 27 , , , cord-269867-k10siwur 101 28 3b,5mAb 3b,5mab CD cord-269867-k10siwur 101 29 and and CC cord-269867-k10siwur 101 30 1AF10mAb 1af10mab CD cord-269867-k10siwur 101 31 ( ( -LRB- cord-269867-k10siwur 101 32 Fig Fig NNP cord-269867-k10siwur 101 33 . . . cord-269867-k10siwur 101 34 4a 4a CD cord-269867-k10siwur 101 35 , , , cord-269867-k10siwur 101 36 b b NN cord-269867-k10siwur 101 37 , , , cord-269867-k10siwur 101 38 c c NNP cord-269867-k10siwur 101 39 ) ) -RRB- cord-269867-k10siwur 101 40 . . . cord-269867-k10siwur 102 1 Mutants mutant NNS cord-269867-k10siwur 102 2 ClpG203-A clpg203-a NN cord-269867-k10siwur 102 3 , , , cord-269867-k10siwur 103 1 ClpG203-CA ClpG203-CA NNP cord-269867-k10siwur 103 2 and and CC cord-269867-k10siwur 103 3 CIpG202-AC CIpG202-AC NNP cord-269867-k10siwur 103 4 showed show VBD cord-269867-k10siwur 103 5 two two CD cord-269867-k10siwur 103 6 protein protein NN cord-269867-k10siwur 103 7 products product NNS cord-269867-k10siwur 103 8 , , , cord-269867-k10siwur 103 9 all all DT cord-269867-k10siwur 103 10 reacting react VBG cord-269867-k10siwur 103 11 with with IN cord-269867-k10siwur 103 12 anti anti JJ cord-269867-k10siwur 103 13 - - JJ cord-269867-k10siwur 103 14 ClpG ClpG NNP cord-269867-k10siwur 103 15 ( ( -LRB- cord-269867-k10siwur 103 16 Fig fig NN cord-269867-k10siwur 103 17 . . . cord-269867-k10siwur 103 18 4a 4a LS cord-269867-k10siwur 103 19 ) ) -RRB- cord-269867-k10siwur 103 20 ; ; : cord-269867-k10siwur 103 21 their -PRON- PRP$ cord-269867-k10siwur 103 22 upper upper JJ cord-269867-k10siwur 103 23 band band NN cord-269867-k10siwur 103 24 detected detect VBN cord-269867-k10siwur 103 25 by by IN cord-269867-k10siwur 103 26 the the DT cord-269867-k10siwur 103 27 three three CD cord-269867-k10siwur 103 28 Ab Ab NNP cord-269867-k10siwur 103 29 represents represent VBZ cord-269867-k10siwur 103 30 the the DT cord-269867-k10siwur 103 31 whole whole JJ cord-269867-k10siwur 103 32 fusion fusion NN cord-269867-k10siwur 103 33 molecule molecule NN cord-269867-k10siwur 103 34 which which WDT cord-269867-k10siwur 103 35 was be VBD cord-269867-k10siwur 103 36 more more RBR cord-269867-k10siwur 103 37 abundant abundant JJ cord-269867-k10siwur 103 38 in in IN cord-269867-k10siwur 103 39 ClpG203-CA ClpG203-CA NNP cord-269867-k10siwur 103 40 . . . cord-269867-k10siwur 104 1 In in IN cord-269867-k10siwur 104 2 ClpG203A clpg203a NN cord-269867-k10siwur 104 3 and and CC cord-269867-k10siwur 104 4 ClpG202-AC ClpG202-AC NNP cord-269867-k10siwur 104 5 , , , cord-269867-k10siwur 104 6 the the DT cord-269867-k10siwur 104 7 major major JJ cord-269867-k10siwur 104 8 lower low JJR cord-269867-k10siwur 104 9 band band NN cord-269867-k10siwur 104 10 was be VBD cord-269867-k10siwur 104 11 recognized recognize VBN cord-269867-k10siwur 104 12 by by IN cord-269867-k10siwur 104 13 only only JJ cord-269867-k10siwur 104 14 anti anti NNS cord-269867-k10siwur 104 15 - - NNS cord-269867-k10siwur 104 16 ClpG ClpG NNP cord-269867-k10siwur 104 17 while while IN cord-269867-k10siwur 104 18 in in IN cord-269867-k10siwur 104 19 Clp Clp NNP cord-269867-k10siwur 104 20 - - HYPH cord-269867-k10siwur 104 21 G203-CA G203-CA NNP cord-269867-k10siwur 104 22 the the DT cord-269867-k10siwur 104 23 minor minor JJ cord-269867-k10siwur 104 24 lower low JJR cord-269867-k10siwur 104 25 band band NN cord-269867-k10siwur 104 26 was be VBD cord-269867-k10siwur 104 27 additionally additionally RB cord-269867-k10siwur 104 28 by by IN cord-269867-k10siwur 104 29 3b.5 3b.5 CD cord-269867-k10siwur 104 30 mAb mAb NNS cord-269867-k10siwur 104 31 ( ( -LRB- cord-269867-k10siwur 104 32 Fig Fig NNP cord-269867-k10siwur 104 33 . . . cord-269867-k10siwur 104 34 4b 4b CD cord-269867-k10siwur 104 35 ) ) -RRB- cord-269867-k10siwur 104 36 . . . cord-269867-k10siwur 105 1 In in IN cord-269867-k10siwur 105 2 ClpG1/203-CA ClpG1/203-CA NNP cord-269867-k10siwur 105 3 the the DT cord-269867-k10siwur 105 4 two two CD cord-269867-k10siwur 105 5 uppermost uppermost JJ cord-269867-k10siwur 105 6 bands band NNS cord-269867-k10siwur 105 7 were be VBD cord-269867-k10siwur 105 8 lighted light VBN cord-269867-k10siwur 105 9 whatever whatever WDT cord-269867-k10siwur 105 10 Ab ab NN cord-269867-k10siwur 105 11 ( ( -LRB- cord-269867-k10siwur 105 12 Fig Fig NNP cord-269867-k10siwur 105 13 . . . cord-269867-k10siwur 105 14 4a 4a CD cord-269867-k10siwur 105 15 , , , cord-269867-k10siwur 105 16 b b NN cord-269867-k10siwur 105 17 , , , cord-269867-k10siwur 105 18 c c NNP cord-269867-k10siwur 105 19 ) ) -RRB- cord-269867-k10siwur 105 20 and and CC cord-269867-k10siwur 105 21 the the DT cord-269867-k10siwur 105 22 two two CD cord-269867-k10siwur 105 23 lowermost lowermost NN cord-269867-k10siwur 105 24 bands band NNS cord-269867-k10siwur 105 25 only only RB cord-269867-k10siwur 105 26 with with IN cord-269867-k10siwur 105 27 anti anti NNS cord-269867-k10siwur 105 28 - - JJ cord-269867-k10siwur 105 29 ClpG ClpG NNS cord-269867-k10siwur 105 30 and and CC cord-269867-k10siwur 105 31 3b.5 3b.5 CD cord-269867-k10siwur 105 32 Ab Ab NNP cord-269867-k10siwur 105 33 ( ( -LRB- cord-269867-k10siwur 105 34 Fig Fig NNP cord-269867-k10siwur 105 35 . . . cord-269867-k10siwur 105 36 4a 4a CD cord-269867-k10siwur 105 37 , , , cord-269867-k10siwur 105 38 b b LS cord-269867-k10siwur 105 39 ) ) -RRB- cord-269867-k10siwur 105 40 . . . cord-269867-k10siwur 106 1 These these DT cord-269867-k10siwur 106 2 observations observation NNS cord-269867-k10siwur 106 3 indicated indicate VBD cord-269867-k10siwur 106 4 that that IN cord-269867-k10siwur 106 5 most most JJS cord-269867-k10siwur 106 6 of of IN cord-269867-k10siwur 106 7 the the DT cord-269867-k10siwur 106 8 hybrids hybrid NNS cord-269867-k10siwur 106 9 carrying carry VBG cord-269867-k10siwur 106 10 TGEV TGEV NNP cord-269867-k10siwur 106 11 - - HYPH cord-269867-k10siwur 106 12 A A NNP cord-269867-k10siwur 106 13 was be VBD cord-269867-k10siwur 106 14 subjected subject VBN cord-269867-k10siwur 106 15 to to IN cord-269867-k10siwur 106 16 an an DT cord-269867-k10siwur 106 17 incomplete incomplete JJ cord-269867-k10siwur 106 18 proteolysis proteolysis NN cord-269867-k10siwur 106 19 which which WDT cord-269867-k10siwur 106 20 , , , cord-269867-k10siwur 106 21 however however RB cord-269867-k10siwur 106 22 , , , cord-269867-k10siwur 106 23 did do VBD cord-269867-k10siwur 106 24 not not RB cord-269867-k10siwur 106 25 prevent prevent VB cord-269867-k10siwur 106 26 the the DT cord-269867-k10siwur 106 27 CS31A cs31a NN cord-269867-k10siwur 106 28 formation formation NN cord-269867-k10siwur 106 29 ( ( -LRB- cord-269867-k10siwur 106 30 Fig Fig NNP cord-269867-k10siwur 106 31 . . . cord-269867-k10siwur 106 32 3 3 CD cord-269867-k10siwur 106 33 and and CC cord-269867-k10siwur 106 34 Section2.2 section2.2 CD cord-269867-k10siwur 106 35 ) ) -RRB- cord-269867-k10siwur 106 36 ) ) -RRB- cord-269867-k10siwur 106 37 . . . cord-269867-k10siwur 107 1 In in IN cord-269867-k10siwur 107 2 a a DT cord-269867-k10siwur 107 3 general general JJ cord-269867-k10siwur 107 4 way way NN cord-269867-k10siwur 107 5 , , , cord-269867-k10siwur 107 6 ClpG ClpG NNP cord-269867-k10siwur 107 7 hybrids hybrid NNS cord-269867-k10siwur 107 8 containing contain VBG cord-269867-k10siwur 107 9 TGEV TGEV NNP cord-269867-k10siwur 107 10 - - HYPH cord-269867-k10siwur 107 11 A A NNP cord-269867-k10siwur 107 12 reacted react VBD cord-269867-k10siwur 107 13 faintly faintly RB cord-269867-k10siwur 107 14 with with IN cord-269867-k10siwur 107 15 1AFI0 1afi0 CD cord-269867-k10siwur 107 16 mAb mAb NNS cord-269867-k10siwur 107 17 ( ( -LRB- cord-269867-k10siwur 107 18 Fig Fig NNP cord-269867-k10siwur 107 19 . . NNP cord-269867-k10siwur 107 20 4c 4c CD cord-269867-k10siwur 107 21 ) ) -RRB- cord-269867-k10siwur 107 22 . . . cord-269867-k10siwur 108 1 peptides peptide NNS cord-269867-k10siwur 108 2 C C NNP cord-269867-k10siwur 108 3 and and CC cord-269867-k10siwur 108 4 A a NN cord-269867-k10siwur 108 5 in in IN cord-269867-k10siwur 108 6 tandem tandem NN cord-269867-k10siwur 108 7 ; ; . cord-269867-k10siwur 109 1 X x NN cord-269867-k10siwur 109 2 , , , cord-269867-k10siwur 109 3 cryptic cryptic JJ cord-269867-k10siwur 109 4 peptide peptide NN cord-269867-k10siwur 109 5 encoded encode VBN cord-269867-k10siwur 109 6 by by IN cord-269867-k10siwur 109 7 oligo oligo NNP cord-269867-k10siwur 109 8 # # $ cord-269867-k10siwur 109 9 3 3 CD cord-269867-k10siwur 110 1 ( ( -LRB- cord-269867-k10siwur 110 2 Fig Fig NNP cord-269867-k10siwur 110 3 . . NNP cord-269867-k10siwur 110 4 2 2 LS cord-269867-k10siwur 110 5 ) ) -RRB- cord-269867-k10siwur 110 6 inserted insert VBN cord-269867-k10siwur 110 7 in in IN cord-269867-k10siwur 110 8 - - HYPH cord-269867-k10siwur 110 9 phase phase NN cord-269867-k10siwur 110 10 but but CC cord-269867-k10siwur 110 11 in in IN cord-269867-k10siwur 110 12 the the DT cord-269867-k10siwur 110 13 reverse reverse JJ cord-269867-k10siwur 110 14 orientation orientation NN cord-269867-k10siwur 110 15 . . . cord-269867-k10siwur 111 1 ( ( -LRB- cord-269867-k10siwur 111 2 d d LS cord-269867-k10siwur 111 3 ) ) -RRB- cord-269867-k10siwur 111 4 aa aa NNP cord-269867-k10siwur 111 5 changes change NNS cord-269867-k10siwur 111 6 resulting result VBG cord-269867-k10siwur 111 7 from from IN cord-269867-k10siwur 111 8 the the DT cord-269867-k10siwur 111 9 engineering engineering NN cord-269867-k10siwur 111 10 of of IN cord-269867-k10siwur 111 11 the the DT cord-269867-k10siwur 111 12 TGEV TGEV NNP cord-269867-k10siwur 111 13 epitopes epitopes NN cord-269867-k10siwur 111 14 - - HYPH cord-269867-k10siwur 111 15 encoding encode VBG cord-269867-k10siwur 111 16 oligos oligo NNS cord-269867-k10siwur 111 17 ( ( -LRB- cord-269867-k10siwur 111 18 Fig fig NN cord-269867-k10siwur 111 19 . . NNP cord-269867-k10siwur 111 20 2 2 CD cord-269867-k10siwur 111 21 ) ) -RRB- cord-269867-k10siwur 111 22 in in IN cord-269867-k10siwur 111 23 CIpG CIpG NNP cord-269867-k10siwur 112 1 : : : cord-269867-k10siwur 112 2 the the DT cord-269867-k10siwur 112 3 numbers number NNS cord-269867-k10siwur 112 4 refer refer VBP cord-269867-k10siwur 112 5 to to IN cord-269867-k10siwur 112 6 the the DT cord-269867-k10siwur 112 7 indicated indicate VBN cord-269867-k10siwur 112 8 first first RB cord-269867-k10siwur 112 9 and and CC cord-269867-k10siwur 112 10 last last JJ cord-269867-k10siwur 112 11 aa aa NNP cord-269867-k10siwur 112 12 residues residue NNS cord-269867-k10siwur 112 13 of of IN cord-269867-k10siwur 112 14 the the DT cord-269867-k10siwur 112 15 wild wild JJ cord-269867-k10siwur 112 16 - - HYPH cord-269867-k10siwur 112 17 type type NN cord-269867-k10siwur 112 18 ( ( -LRB- cord-269867-k10siwur 112 19 wt wt NN cord-269867-k10siwur 112 20 ) ) -RRB- cord-269867-k10siwur 112 21 CIpG CIpG NNP cord-269867-k10siwur 112 22 sequence sequence NN cord-269867-k10siwur 112 23 ; ; : cord-269867-k10siwur 112 24 residues residue NNS cord-269867-k10siwur 112 25 from from IN cord-269867-k10siwur 112 26 the the DT cord-269867-k10siwur 112 27 original original JJ cord-269867-k10siwur 112 28 ClpG ClpG NNP cord-269867-k10siwur 112 29 protein protein NN cord-269867-k10siwur 112 30 are be VBP cord-269867-k10siwur 112 31 in in IN cord-269867-k10siwur 112 32 small small JJ cord-269867-k10siwur 112 33 characters character NNS cord-269867-k10siwur 112 34 and and CC cord-269867-k10siwur 112 35 additional additional JJ cord-269867-k10siwur 112 36 residues residue NNS cord-269867-k10siwur 112 37 are be VBP cord-269867-k10siwur 112 38 in in IN cord-269867-k10siwur 112 39 large large JJ cord-269867-k10siwur 112 40 bold bold JJ cord-269867-k10siwur 112 41 type type NN cord-269867-k10siwur 112 42 . . . cord-269867-k10siwur 113 1 Hatched hatch VBN cord-269867-k10siwur 113 2 boxes box NNS cord-269867-k10siwur 113 3 , , , cord-269867-k10siwur 113 4 TGEV TGEV NNP cord-269867-k10siwur 113 5 - - HYPH cord-269867-k10siwur 113 6 C C NNP cord-269867-k10siwur 113 7 ; ; , cord-269867-k10siwur 113 8 black black JJ cord-269867-k10siwur 113 9 boxes box NNS cord-269867-k10siwur 113 10 , , , cord-269867-k10siwur 113 11 TGEV TGEV NNP cord-269867-k10siwur 113 12 - - HYPH cord-269867-k10siwur 113 13 A a NN cord-269867-k10siwur 113 14 ; ; , cord-269867-k10siwur 113 15 open open JJ cord-269867-k10siwur 113 16 boxes box NNS cord-269867-k10siwur 113 17 , , , cord-269867-k10siwur 113 18 cryptic cryptic JJ cord-269867-k10siwur 113 19 peptide peptide NN cord-269867-k10siwur 113 20 X x NN cord-269867-k10siwur 113 21 : : : cord-269867-k10siwur 113 22 TQQADHSQISS TQQADHSQISS NNP cord-269867-k10siwur 113 23 . . . cord-269867-k10siwur 114 1 ( ( -LRB- cord-269867-k10siwur 114 2 e e LS cord-269867-k10siwur 114 3 ) ) -RRB- cord-269867-k10siwur 114 4 Total total JJ cord-269867-k10siwur 114 5 number number NN cord-269867-k10siwur 114 6 of of IN cord-269867-k10siwur 114 7 added add VBN cord-269867-k10siwur 114 8 aa aa NNP cord-269867-k10siwur 114 9 with with IN cord-269867-k10siwur 114 10 respect respect NN cord-269867-k10siwur 114 11 to to IN cord-269867-k10siwur 114 12 wt wt NN cord-269867-k10siwur 114 13 CIpG. CIpG. NNP cord-269867-k10siwur 114 14 ( ( -LRB- cord-269867-k10siwur 115 1 f f LS cord-269867-k10siwur 115 2 ) ) -RRB- cord-269867-k10siwur 115 3 CS31A CS31A NNP cord-269867-k10siwur 115 4 fibrillae fibrillae NNP cord-269867-k10siwur 115 5 biogenesis biogenesis NN cord-269867-k10siwur 115 6 : : : cord-269867-k10siwur 116 1 + + CC cord-269867-k10siwur 116 2 and and CC cord-269867-k10siwur 116 3 - - HYPH cord-269867-k10siwur 116 4 , , , cord-269867-k10siwur 116 5 synthesis synthesis NN cord-269867-k10siwur 116 6 and and CC cord-269867-k10siwur 116 7 no no DT cord-269867-k10siwur 116 8 synthesis synthesis NN cord-269867-k10siwur 116 9 , , , cord-269867-k10siwur 116 10 respectively respectively RB cord-269867-k10siwur 116 11 . . . cord-269867-k10siwur 117 1 Methods method NNS cord-269867-k10siwur 117 2 : : : cord-269867-k10siwur 117 3 The the DT cord-269867-k10siwur 117 4 production production NN cord-269867-k10siwur 117 5 of of IN cord-269867-k10siwur 117 6 hybrid hybrid JJ cord-269867-k10siwur 117 7 CS31A cs31a NN cord-269867-k10siwur 117 8 polymers polymer NNS cord-269867-k10siwur 117 9 was be VBD cord-269867-k10siwur 117 10 detected detect VBN cord-269867-k10siwur 117 11 by by IN cord-269867-k10siwur 117 12 in in IN cord-269867-k10siwur 117 13 situ situ JJ cord-269867-k10siwur 117 14 colony colony NN cord-269867-k10siwur 117 15 immunoblotting immunoblotting NN cord-269867-k10siwur 117 16 and and CC cord-269867-k10siwur 117 17 immunodot immunodot NNP cord-269867-k10siwur 117 18 analysis analysis NN cord-269867-k10siwur 117 19 . . . cord-269867-k10siwur 118 1 For for IN cord-269867-k10siwur 118 2 colony colony NN cord-269867-k10siwur 118 3 blots blot NNS cord-269867-k10siwur 118 4 analysis analysis NN cord-269867-k10siwur 118 5 , , , cord-269867-k10siwur 118 6 single single JJ cord-269867-k10siwur 118 7 colonies colony NNS cord-269867-k10siwur 118 8 were be VBD cord-269867-k10siwur 118 9 streaked streak VBN cord-269867-k10siwur 118 10 on on IN cord-269867-k10siwur 118 11 a a DT cord-269867-k10siwur 118 12 solid solid JJ cord-269867-k10siwur 118 13 agar agar JJ cord-269867-k10siwur 118 14 LB LB NNP cord-269867-k10siwur 118 15 plate plate NN cord-269867-k10siwur 118 16 containing contain VBG cord-269867-k10siwur 118 17 appropriate appropriate JJ cord-269867-k10siwur 118 18 antibiotics antibiotic NNS cord-269867-k10siwur 118 19 . . . cord-269867-k10siwur 119 1 After after IN cord-269867-k10siwur 119 2 overnight overnight JJ cord-269867-k10siwur 119 3 incubation incubation NN cord-269867-k10siwur 119 4 at at IN cord-269867-k10siwur 119 5 37 37 CD cord-269867-k10siwur 119 6 ° ° NN cord-269867-k10siwur 119 7 C c NN cord-269867-k10siwur 119 8 , , , cord-269867-k10siwur 119 9 a a DT cord-269867-k10siwur 119 10 nitrocellulose nitrocellulose NN cord-269867-k10siwur 119 11 filter filter NN cord-269867-k10siwur 119 12 ( ( -LRB- cord-269867-k10siwur 119 13 pore pore NN cord-269867-k10siwur 119 14 diameter diameter NN cord-269867-k10siwur 119 15 , , , cord-269867-k10siwur 119 16 0.45 0.45 CD cord-269867-k10siwur 119 17 ~tm ~tm NNP cord-269867-k10siwur 119 18 ; ; : cord-269867-k10siwur 119 19 Schleicher Schleicher NNP cord-269867-k10siwur 119 20 and and CC cord-269867-k10siwur 119 21 Schuell Schuell NNP cord-269867-k10siwur 119 22 ) ) -RRB- cord-269867-k10siwur 119 23 was be VBD cord-269867-k10siwur 119 24 carefully carefully RB cord-269867-k10siwur 119 25 applied apply VBN cord-269867-k10siwur 119 26 on on IN cord-269867-k10siwur 119 27 agar agar NN cord-269867-k10siwur 119 28 surface surface NN cord-269867-k10siwur 119 29 . . . cord-269867-k10siwur 120 1 Blots blot NNS cord-269867-k10siwur 120 2 were be VBD cord-269867-k10siwur 120 3 blocked block VBN cord-269867-k10siwur 120 4 and and CC cord-269867-k10siwur 120 5 then then RB cord-269867-k10siwur 120 6 washed wash VBD cord-269867-k10siwur 120 7 with with IN cord-269867-k10siwur 120 8 1 1 CD cord-269867-k10siwur 120 9 % % NN cord-269867-k10siwur 120 10 BSA-0.01 bsa-0.01 JJ cord-269867-k10siwur 120 11 % % NN cord-269867-k10siwur 120 12 Tween Tween NNP cord-269867-k10siwur 120 13 20 20 CD cord-269867-k10siwur 120 14 in in IN cord-269867-k10siwur 120 15 PBS PBS NNP cord-269867-k10siwur 120 16 until until IN cord-269867-k10siwur 120 17 the the DT cord-269867-k10siwur 120 18 bulk bulk NN cord-269867-k10siwur 120 19 of of IN cord-269867-k10siwur 120 20 bacteria bacteria NNS cord-269867-k10siwur 120 21 was be VBD cord-269867-k10siwur 120 22 removed remove VBN cord-269867-k10siwur 120 23 . . . cord-269867-k10siwur 121 1 The the DT cord-269867-k10siwur 121 2 filters filter NNS cord-269867-k10siwur 121 3 were be VBD cord-269867-k10siwur 121 4 further further RB cord-269867-k10siwur 121 5 incubated incubate VBN cord-269867-k10siwur 121 6 with with IN cord-269867-k10siwur 121 7 appropriate appropriate JJ cord-269867-k10siwur 121 8 primary primary JJ cord-269867-k10siwur 121 9 Ab ab NN cord-269867-k10siwur 121 10 in in IN cord-269867-k10siwur 121 11 1 1 CD cord-269867-k10siwur 121 12 % % NN cord-269867-k10siwur 121 13 BSA BSA NNP cord-269867-k10siwur 121 14 in in IN cord-269867-k10siwur 121 15 PBS PBS NNP cord-269867-k10siwur 121 16 . . . cord-269867-k10siwur 122 1 Bound bind VBN cord-269867-k10siwur 122 2 primary primary JJ cord-269867-k10siwur 122 3 Ab Ab NNP cord-269867-k10siwur 122 4 were be VBD cord-269867-k10siwur 122 5 detected detect VBN cord-269867-k10siwur 122 6 by by IN cord-269867-k10siwur 122 7 incubation incubation NN cord-269867-k10siwur 122 8 of of IN cord-269867-k10siwur 122 9 the the DT cord-269867-k10siwur 122 10 filters filter NNS cord-269867-k10siwur 122 11 with with IN cord-269867-k10siwur 122 12 either either CC cord-269867-k10siwur 122 13 horseradish horseradish NN cord-269867-k10siwur 122 14 peroxidase peroxidase NN cord-269867-k10siwur 122 15 - - HYPH cord-269867-k10siwur 122 16 conjugated conjugate VBN cord-269867-k10siwur 122 17 anti anti JJ cord-269867-k10siwur 122 18 - - JJ cord-269867-k10siwur 122 19 rabbit rabbit JJ cord-269867-k10siwur 122 20 or or CC cord-269867-k10siwur 122 21 anti anti JJ cord-269867-k10siwur 122 22 - - JJ cord-269867-k10siwur 122 23 mouse mouse NN cord-269867-k10siwur 122 24 secondary secondary JJ cord-269867-k10siwur 122 25 Ab Ab NNP cord-269867-k10siwur 122 26 , , , cord-269867-k10siwur 122 27 and and CC cord-269867-k10siwur 122 28 developed develop VBD cord-269867-k10siwur 122 29 with with IN cord-269867-k10siwur 122 30 H202-~-chloronaphthol H202-~-chloronaphthol NNP cord-269867-k10siwur 122 31 . . . cord-269867-k10siwur 123 1 For for IN cord-269867-k10siwur 123 2 the the DT cord-269867-k10siwur 123 3 immunodot immunodot NNP cord-269867-k10siwur 123 4 analysis analysis NN cord-269867-k10siwur 123 5 of of IN cord-269867-k10siwur 123 6 the the DT cord-269867-k10siwur 123 7 extracted extract VBN cord-269867-k10siwur 123 8 mutated mutate VBN cord-269867-k10siwur 123 9 CS31A cs31a NN cord-269867-k10siwur 123 10 fibrillae fibrillae NNP cord-269867-k10siwur 123 11 , , , cord-269867-k10siwur 123 12 bacteria bacteria NNS cord-269867-k10siwur 123 13 growing grow VBG cord-269867-k10siwur 123 14 overnight overnight RB cord-269867-k10siwur 123 15 on on IN cord-269867-k10siwur 123 16 LB LB NNP cord-269867-k10siwur 123 17 agar agar NN cord-269867-k10siwur 123 18 medium medium NN cord-269867-k10siwur 123 19 with with IN cord-269867-k10siwur 123 20 the the DT cord-269867-k10siwur 123 21 appropriate appropriate JJ cord-269867-k10siwur 123 22 antibiotics antibiotic NNS cord-269867-k10siwur 123 23 were be VBD cord-269867-k10siwur 123 24 carefully carefully RB cord-269867-k10siwur 123 25 scraped scrape VBN cord-269867-k10siwur 123 26 and and CC cord-269867-k10siwur 123 27 suspended suspend VBN cord-269867-k10siwur 123 28 in in IN cord-269867-k10siwur 123 29 PBS PBS NNP cord-269867-k10siwur 123 30 . . . cord-269867-k10siwur 124 1 This this DT cord-269867-k10siwur 124 2 suspension suspension NN cord-269867-k10siwur 124 3 was be VBD cord-269867-k10siwur 124 4 then then RB cord-269867-k10siwur 124 5 vigorously vigorously RB cord-269867-k10siwur 124 6 agitated agitated JJ cord-269867-k10siwur 124 7 for for IN cord-269867-k10siwur 124 8 ] ] -RRB- cord-269867-k10siwur 124 9 [ [ -LRB- cord-269867-k10siwur 124 10 min min NN cord-269867-k10siwur 124 11 with with IN cord-269867-k10siwur 124 12 a a DT cord-269867-k10siwur 124 13 top top JJ cord-269867-k10siwur 124 14 mix mix NN cord-269867-k10siwur 124 15 shaker shaker NN cord-269867-k10siwur 124 16 , , , cord-269867-k10siwur 124 17 and and CC cord-269867-k10siwur 124 18 placed place VBN cord-269867-k10siwur 124 19 at at IN cord-269867-k10siwur 124 20 60 60 CD cord-269867-k10siwur 124 21 ° ° NN cord-269867-k10siwur 124 22 C c NN cord-269867-k10siwur 124 23 for for IN cord-269867-k10siwur 124 24 20 20 CD cord-269867-k10siwur 124 25 min min NN cord-269867-k10siwur 124 26 ( ( -LRB- cord-269867-k10siwur 124 27 thermo thermo NNP cord-269867-k10siwur 124 28 - - HYPH cord-269867-k10siwur 124 29 elution elution NNP cord-269867-k10siwur 124 30 of of IN cord-269867-k10siwur 124 31 the the DT cord-269867-k10siwur 124 32 CS31A CS31A NNP cord-269867-k10siwur 124 33 polymer polymer NNP cord-269867-k10siwur 124 34 ) ) -RRB- cord-269867-k10siwur 124 35 . . . cord-269867-k10siwur 125 1 After after IN cord-269867-k10siwur 125 2 centrifugation centrifugation NN cord-269867-k10siwur 125 3 at at IN cord-269867-k10siwur 125 4 12 12 CD cord-269867-k10siwur 125 5 000 000 CD cord-269867-k10siwur 125 6 x x NN cord-269867-k10siwur 125 7 g g NN cord-269867-k10siwur 125 8 for for IN cord-269867-k10siwur 125 9 10 10 CD cord-269867-k10siwur 125 10 min min NN cord-269867-k10siwur 125 11 , , , cord-269867-k10siwur 125 12 the the DT cord-269867-k10siwur 125 13 resulting result VBG cord-269867-k10siwur 125 14 supernatant supernatant NN cord-269867-k10siwur 125 15 was be VBD cord-269867-k10siwur 125 16 used use VBN cord-269867-k10siwur 125 17 for for IN cord-269867-k10siwur 125 18 experiments experiment NNS cord-269867-k10siwur 125 19 . . . cord-269867-k10siwur 126 1 CS31A CS31A NNP cord-269867-k10siwur 127 1 fibrillae fibrillae NNP cord-269867-k10siwur 127 2 - - HYPH cord-269867-k10siwur 127 3 specific specific JJ cord-269867-k10siwur 127 4 rabbit rabbit NN cord-269867-k10siwur 127 5 antiserum antiserum NN cord-269867-k10siwur 127 6 ( ( -LRB- cord-269867-k10siwur 127 7 anti anti JJ cord-269867-k10siwur 127 8 - - JJ cord-269867-k10siwur 127 9 CS3lA cs3la JJ cord-269867-k10siwur 127 10 ) ) -RRB- cord-269867-k10siwur 127 11 was be VBD cord-269867-k10siwur 127 12 obtained obtain VBN cord-269867-k10siwur 127 13 as as IN cord-269867-k10siwur 127 14 described describe VBN cord-269867-k10siwur 127 15 by by IN cord-269867-k10siwur 127 16 Girardeau Girardeau NNP cord-269867-k10siwur 127 17 et et NNP cord-269867-k10siwur 127 18 al al NNP cord-269867-k10siwur 127 19 . . . cord-269867-k10siwur 128 1 ( ( -LRB- cord-269867-k10siwur 128 2 1988 1988 CD cord-269867-k10siwur 128 3 ) ) -RRB- cord-269867-k10siwur 128 4 . . . cord-269867-k10siwur 129 1 mAb mab NN cord-269867-k10siwur 129 2 3b.5 3b.5 CD cord-269867-k10siwur 129 3 ( ( -LRB- cord-269867-k10siwur 129 4 Delmas Delmas NNP cord-269867-k10siwur 129 5 et et NNP cord-269867-k10siwur 129 6 al al NNP cord-269867-k10siwur 129 7 . . NNP cord-269867-k10siwur 129 8 , , , cord-269867-k10siwur 129 9 1990 1990 CD cord-269867-k10siwur 129 10 ) ) -RRB- cord-269867-k10siwur 129 11 and and CC cord-269867-k10siwur 129 12 mAb mab NN cord-269867-k10siwur 129 13 1AF10 1af10 CD cord-269867-k10siwur 130 1 ( ( -LRB- cord-269867-k10siwur 130 2 Gebauer Gebauer NNP cord-269867-k10siwur 130 3 et et NNP cord-269867-k10siwur 130 4 al al NNP cord-269867-k10siwur 130 5 . . NNP cord-269867-k10siwur 130 6 , , , cord-269867-k10siwur 130 7 1991 1991 CD cord-269867-k10siwur 130 8 ) ) -RRB- cord-269867-k10siwur 130 9 raised raise VBD cord-269867-k10siwur 130 10 , , , cord-269867-k10siwur 130 11 respectively respectively RB cord-269867-k10siwur 130 12 , , , cord-269867-k10siwur 130 13 against against IN cord-269867-k10siwur 130 14 the the DT cord-269867-k10siwur 130 15 C C NNP cord-269867-k10siwur 130 16 and and CC cord-269867-k10siwur 130 17 A a DT cord-269867-k10siwur 130 18 sites site NNS cord-269867-k10siwur 130 19 of of IN cord-269867-k10siwur 130 20 TGEV TGEV NNP cord-269867-k10siwur 130 21 - - HYPH cord-269867-k10siwur 130 22 S S NNP cord-269867-k10siwur 130 23 on on IN cord-269867-k10siwur 130 24 native native JJ cord-269867-k10siwur 130 25 coronavirus coronavirus NN cord-269867-k10siwur 130 26 , , , cord-269867-k10siwur 130 27 were be VBD cord-269867-k10siwur 130 28 used use VBN cord-269867-k10siwur 130 29 . . . cord-269867-k10siwur 131 1 Fig Fig NNP cord-269867-k10siwur 131 2 . . . cord-269867-k10siwur 132 1 3 3 CD cord-269867-k10siwur 132 2 legend legend NN cord-269867-k10siwur 132 3 ) ) -RRB- cord-269867-k10siwur 132 4 were be VBD cord-269867-k10siwur 132 5 mixed mix VBN cord-269867-k10siwur 132 6 with with IN cord-269867-k10siwur 132 7 an an DT cord-269867-k10siwur 132 8 equal equal JJ cord-269867-k10siwur 132 9 volume volume NN cord-269867-k10siwur 132 10 of of IN cord-269867-k10siwur 132 11 2 2 CD cord-269867-k10siwur 132 12 x x SYM cord-269867-k10siwur 132 13 Laemmli Laemmli NNP cord-269867-k10siwur 132 14 buffer buffer NN cord-269867-k10siwur 132 15 , , , cord-269867-k10siwur 132 16 boiled boil VBN cord-269867-k10siwur 132 17 for for IN cord-269867-k10siwur 132 18 5 5 CD cord-269867-k10siwur 132 19 min min NN cord-269867-k10siwur 132 20 , , , cord-269867-k10siwur 132 21 separated separate VBN cord-269867-k10siwur 132 22 by by IN cord-269867-k10siwur 132 23 15 15 CD cord-269867-k10siwur 132 24 % % NN cord-269867-k10siwur 132 25 SDS SDS NNP cord-269867-k10siwur 132 26 - - HYPH cord-269867-k10siwur 132 27 PAGE PAGE NNP cord-269867-k10siwur 132 28 and and CC cord-269867-k10siwur 132 29 semi semi JJ cord-269867-k10siwur 132 30 - - JJ cord-269867-k10siwur 132 31 dry dry JJ cord-269867-k10siwur 132 32 electrotransferred electrotransferre VBN cord-269867-k10siwur 132 33 onto onto IN cord-269867-k10siwur 132 34 nitrocellulose nitrocellulose NN cord-269867-k10siwur 132 35 sheets sheet NNS cord-269867-k10siwur 133 1 ( ( -LRB- cord-269867-k10siwur 133 2 Towbin Towbin NNP cord-269867-k10siwur 133 3 et et NNP cord-269867-k10siwur 133 4 al al NNP cord-269867-k10siwur 133 5 . . NNP cord-269867-k10siwur 133 6 , , , cord-269867-k10siwur 133 7 1979 1979 CD cord-269867-k10siwur 133 8 ) ) -RRB- cord-269867-k10siwur 133 9 . . . cord-269867-k10siwur 134 1 Control Control NNP cord-269867-k10siwur 134 2 , , , cord-269867-k10siwur 134 3 wt wt NNP cord-269867-k10siwur 134 4 CS31A CS31A NNP cord-269867-k10siwur 134 5 fibrillae fibrillae NNP cord-269867-k10siwur 134 6 produced produce VBN cord-269867-k10siwur 134 7 by by IN cord-269867-k10siwur 134 8 E. E. NNP cord-269867-k10siwur 134 9 coil coil NN cord-269867-k10siwur 134 10 DH5e DH5e NNP cord-269867-k10siwur 134 11 [ [ -LRB- cord-269867-k10siwur 134 12 pDEV41155 pdev41155 NN cord-269867-k10siwur 134 13 , , , cord-269867-k10siwur 134 14 pDSPH524 pDSPH524 NNP cord-269867-k10siwur 134 15 ] ] -RRB- cord-269867-k10siwur 134 16 . . . cord-269867-k10siwur 135 1 Western western JJ cord-269867-k10siwur 135 2 blots blot NNS cord-269867-k10siwur 135 3 were be VBD cord-269867-k10siwur 135 4 then then RB cord-269867-k10siwur 135 5 treated treat VBN cord-269867-k10siwur 135 6 as as IN cord-269867-k10siwur 135 7 described describe VBN cord-269867-k10siwur 135 8 in in IN cord-269867-k10siwur 135 9 the the DT cord-269867-k10siwur 135 10 Fig Fig NNP cord-269867-k10siwur 135 11 . . . cord-269867-k10siwur 136 1 3 3 CD cord-269867-k10siwur 136 2 legend legend NN cord-269867-k10siwur 136 3 by by IN cord-269867-k10siwur 136 4 using use VBG cord-269867-k10siwur 136 5 as as IN cord-269867-k10siwur 136 6 primary primary JJ cord-269867-k10siwur 136 7 Ab ab NN cord-269867-k10siwur 136 8 either either CC cord-269867-k10siwur 136 9 ( ( -LRB- cord-269867-k10siwur 136 10 a a DT cord-269867-k10siwur 136 11 ) ) -RRB- cord-269867-k10siwur 136 12 anti anti JJ cord-269867-k10siwur 136 13 - - JJ cord-269867-k10siwur 136 14 ClpG ClpG NNP cord-269867-k10siwur 136 15 pAb pab NN cord-269867-k10siwur 136 16 , , , cord-269867-k10siwur 136 17 or or CC cord-269867-k10siwur 137 1 ( ( -LRB- cord-269867-k10siwur 137 2 b b NN cord-269867-k10siwur 137 3 ) ) -RRB- cord-269867-k10siwur 137 4 3b.5 3b.5 CD cord-269867-k10siwur 138 1 mAb mAb NNS cord-269867-k10siwur 138 2 , , , cord-269867-k10siwur 138 3 or or CC cord-269867-k10siwur 139 1 ( ( -LRB- cord-269867-k10siwur 139 2 c c NN cord-269867-k10siwur 139 3 ) ) -RRB- cord-269867-k10siwur 139 4 1AF10 1af10 CD cord-269867-k10siwur 140 1 mAb mAb NNS cord-269867-k10siwur 140 2 , , , cord-269867-k10siwur 140 3 or or CC cord-269867-k10siwur 141 1 ( ( -LRB- cord-269867-k10siwur 141 2 d d LS cord-269867-k10siwur 141 3 ) ) -RRB- cord-269867-k10siwur 141 4 anti anti JJ cord-269867-k10siwur 141 5 - - JJ cord-269867-k10siwur 141 6 G15Q g15q JJ cord-269867-k10siwur 141 7 pAb pab NN cord-269867-k10siwur 141 8 , , , cord-269867-k10siwur 141 9 or or CC cord-269867-k10siwur 141 10 ( ( -LRB- cord-269867-k10siwur 141 11 e e LS cord-269867-k10siwur 141 12 ) ) -RRB- cord-269867-k10siwur 141 13 anti anti JJ cord-269867-k10siwur 141 14 - - JJ cord-269867-k10siwur 141 15 T15P t15p JJ cord-269867-k10siwur 141 16 pAb pab NN cord-269867-k10siwur 141 17 . . . cord-269867-k10siwur 142 1 ClpG ClpG NNP cord-269867-k10siwur 142 2 subunit subunit NN cord-269867-k10siwur 142 3 - - HYPH cord-269867-k10siwur 142 4 specific specific JJ cord-269867-k10siwur 142 5 rabbit rabbit NN cord-269867-k10siwur 142 6 antiserum antiserum NN cord-269867-k10siwur 142 7 ( ( -LRB- cord-269867-k10siwur 142 8 anti anti NNP cord-269867-k10siwur 142 9 - - JJ cord-269867-k10siwur 142 10 ClpG ClpG NNP cord-269867-k10siwur 142 11 pAb pab NN cord-269867-k10siwur 142 12 ) ) -RRB- cord-269867-k10siwur 142 13 was be VBD cord-269867-k10siwur 142 14 obtained obtain VBN cord-269867-k10siwur 142 15 as as IN cord-269867-k10siwur 142 16 described describe VBN cord-269867-k10siwur 142 17 by by IN cord-269867-k10siwur 142 18 Girardeau Girardeau NNP cord-269867-k10siwur 142 19 et et NNP cord-269867-k10siwur 142 20 al al NNP cord-269867-k10siwur 142 21 . . . cord-269867-k10siwur 143 1 ( ( -LRB- cord-269867-k10siwur 143 2 1988 1988 CD cord-269867-k10siwur 143 3 ) ) -RRB- cord-269867-k10siwur 143 4 . . . cord-269867-k10siwur 144 1 The the DT cord-269867-k10siwur 144 2 G15Q g15q NN cord-269867-k10siwur 144 3 peptide peptide NN cord-269867-k10siwur 144 4 ( ( -LRB- cord-269867-k10siwur 144 5 GQLQAVNPNAGNRGQ GQLQAVNPNAGNRGQ NNP cord-269867-k10siwur 144 6 ) ) -RRB- cord-269867-k10siwur 144 7 and and CC cord-269867-k10siwur 144 8 the the DT cord-269867-k10siwur 144 9 T15P T15P NNP cord-269867-k10siwur 144 10 peptide peptide NN cord-269867-k10siwur 144 11 ( ( -LRB- cord-269867-k10siwur 144 12 TFTNPVVSTTQWSAP TFTNPVVSTTQWSAP NNP cord-269867-k10siwur 144 13 ) ) -RRB- cord-269867-k10siwur 144 14 correspond correspond VBP cord-269867-k10siwur 144 15 to to IN cord-269867-k10siwur 144 16 the the DT cord-269867-k10siwur 144 17 aa aa NNP cord-269867-k10siwur 144 18 residues residue NNS cord-269867-k10siwur 144 19 185 185 CD cord-269867-k10siwur 144 20 - - SYM cord-269867-k10siwur 144 21 199 199 CD cord-269867-k10siwur 144 22 and and CC cord-269867-k10siwur 144 23 235 235 CD cord-269867-k10siwur 144 24 - - SYM cord-269867-k10siwur 144 25 249 249 CD cord-269867-k10siwur 144 26 of of IN cord-269867-k10siwur 144 27 CIpG CIpG NNPS cord-269867-k10siwur 144 28 , , , cord-269867-k10siwur 144 29 respectively respectively RB cord-269867-k10siwur 144 30 . . . cord-269867-k10siwur 145 1 The the DT cord-269867-k10siwur 145 2 synthetic synthetic JJ cord-269867-k10siwur 145 3 G15Q g15q NN cord-269867-k10siwur 145 4 and and CC cord-269867-k10siwur 145 5 T15P t15p NN cord-269867-k10siwur 145 6 peptides peptide NNS cord-269867-k10siwur 145 7 were be VBD cord-269867-k10siwur 145 8 obtained obtain VBN cord-269867-k10siwur 145 9 from from IN cord-269867-k10siwur 145 10 Neosystem Neosystem NNP cord-269867-k10siwur 145 11 ( ( -LRB- cord-269867-k10siwur 145 12 Strasbourg Strasbourg NNP cord-269867-k10siwur 145 13 , , , cord-269867-k10siwur 145 14 France France NNP cord-269867-k10siwur 145 15 ) ) -RRB- cord-269867-k10siwur 145 16 and and CC cord-269867-k10siwur 145 17 their -PRON- PRP$ cord-269867-k10siwur 145 18 purity purity NN cord-269867-k10siwur 145 19 was be VBD cord-269867-k10siwur 145 20 > > : cord-269867-k10siwur 145 21 75 75 CD cord-269867-k10siwur 145 22 % % NN cord-269867-k10siwur 145 23 as as IN cord-269867-k10siwur 145 24 determined determine VBN cord-269867-k10siwur 145 25 by by IN cord-269867-k10siwur 145 26 high high JJ cord-269867-k10siwur 145 27 - - HYPH cord-269867-k10siwur 145 28 performance performance NN cord-269867-k10siwur 145 29 liquid liquid NN cord-269867-k10siwur 145 30 chromatography chromatography NN cord-269867-k10siwur 145 31 . . . cord-269867-k10siwur 146 1 To to TO cord-269867-k10siwur 146 2 prepare prepare VB cord-269867-k10siwur 146 3 anti anti JJ cord-269867-k10siwur 146 4 - - JJ cord-269867-k10siwur 146 5 G15Q g15q JJ cord-269867-k10siwur 146 6 and and CC cord-269867-k10siwur 146 7 anti anti JJ cord-269867-k10siwur 146 8 - - JJ cord-269867-k10siwur 146 9 Tl5P tl5p JJ cord-269867-k10siwur 146 10 antisera antisera NNP cord-269867-k10siwur 146 11 , , , cord-269867-k10siwur 146 12 peptides peptide NNS cord-269867-k10siwur 146 13 G15Q g15q NN cord-269867-k10siwur 146 14 and and CC cord-269867-k10siwur 146 15 T15P T15P NNP cord-269867-k10siwur 146 16 were be VBD cord-269867-k10siwur 146 17 coupled couple VBN cord-269867-k10siwur 146 18 to to IN cord-269867-k10siwur 146 19 BSA BSA NNP cord-269867-k10siwur 146 20 using use VBG cord-269867-k10siwur 146 21 glutaraldehyde glutaraldehyde NN cord-269867-k10siwur 146 22 ( ( -LRB- cord-269867-k10siwur 146 23 Sigma Sigma NNP cord-269867-k10siwur 146 24 ) ) -RRB- cord-269867-k10siwur 146 25 ; ; : cord-269867-k10siwur 146 26 rabbits rabbit NNS cord-269867-k10siwur 146 27 were be VBD cord-269867-k10siwur 146 28 primed prime VBN cord-269867-k10siwur 146 29 by by IN cord-269867-k10siwur 146 30 intradermic intradermic JJ cord-269867-k10siwur 146 31 injection injection NN cord-269867-k10siwur 146 32 of of IN cord-269867-k10siwur 146 33 250 250 CD cord-269867-k10siwur 146 34 gg gg NNS cord-269867-k10siwur 146 35 of of IN cord-269867-k10siwur 146 36 peptide peptide NN cord-269867-k10siwur 146 37 emulsified emulsify VBN cord-269867-k10siwur 146 38 with with IN cord-269867-k10siwur 146 39 incomplete incomplete JJ cord-269867-k10siwur 146 40 Freund Freund NNP cord-269867-k10siwur 146 41 's 's POS cord-269867-k10siwur 146 42 adjuvant adjuvant NN cord-269867-k10siwur 146 43 , , , cord-269867-k10siwur 146 44 then then RB cord-269867-k10siwur 146 45 boosted boost VBD cord-269867-k10siwur 146 46 20 20 CD cord-269867-k10siwur 146 47 and and CC cord-269867-k10siwur 146 48 40 40 CD cord-269867-k10siwur 146 49 days day NNS cord-269867-k10siwur 146 50 later later RB cord-269867-k10siwur 146 51 , , , cord-269867-k10siwur 146 52 and and CC cord-269867-k10siwur 146 53 finally finally RB cord-269867-k10siwur 146 54 bled bleed VBD cord-269867-k10siwur 146 55 15 15 CD cord-269867-k10siwur 146 56 days day NNS cord-269867-k10siwur 146 57 after after IN cord-269867-k10siwur 146 58 the the DT cord-269867-k10siwur 146 59 last last JJ cord-269867-k10siwur 146 60 immunization immunization NN cord-269867-k10siwur 146 61 . . . cord-269867-k10siwur 147 1 Although although IN cord-269867-k10siwur 147 2 we -PRON- PRP cord-269867-k10siwur 147 3 have have VBP cord-269867-k10siwur 147 4 not not RB cord-269867-k10siwur 147 5 sequenced sequence VBN cord-269867-k10siwur 147 6 the the DT cord-269867-k10siwur 147 7 N N NNP cord-269867-k10siwur 147 8 - - HYPH cord-269867-k10siwur 147 9 and and CC cord-269867-k10siwur 147 10 C c NN cord-269867-k10siwur 147 11 - - HYPH cord-269867-k10siwur 147 12 terminal terminal NN cord-269867-k10siwur 147 13 parts part NNS cord-269867-k10siwur 147 14 of of IN cord-269867-k10siwur 147 15 cleaved cleaved JJ cord-269867-k10siwur 147 16 products product NNS cord-269867-k10siwur 147 17 from from IN cord-269867-k10siwur 147 18 CIpG::TGEV CIpG::TGEV NNS cord-269867-k10siwur 147 19 hybrids hybrid NNS cord-269867-k10siwur 147 20 , , , cord-269867-k10siwur 147 21 several several JJ cord-269867-k10siwur 147 22 lines line NNS cord-269867-k10siwur 147 23 of of IN cord-269867-k10siwur 147 24 evidence evidence NN cord-269867-k10siwur 147 25 suggest suggest VBP cord-269867-k10siwur 147 26 that that IN cord-269867-k10siwur 147 27 a a DT cord-269867-k10siwur 147 28 proteolytic proteolytic JJ cord-269867-k10siwur 147 29 event event NN cord-269867-k10siwur 147 30 occurs occur VBZ cord-269867-k10siwur 147 31 within within IN cord-269867-k10siwur 147 32 the the DT cord-269867-k10siwur 147 33 TGEV TGEV NNP cord-269867-k10siwur 147 34 - - HYPH cord-269867-k10siwur 147 35 A a DT cord-269867-k10siwur 147 36 oligopeptide oligopeptide NN cord-269867-k10siwur 147 37 : : : cord-269867-k10siwur 148 1 ( ( -LRB- cord-269867-k10siwur 148 2 1 1 LS cord-269867-k10siwur 148 3 ) ) -RRB- cord-269867-k10siwur 148 4 no no DT cord-269867-k10siwur 148 5 truncated truncated JJ cord-269867-k10siwur 148 6 products product NNS cord-269867-k10siwur 148 7 from from IN cord-269867-k10siwur 148 8 any any DT cord-269867-k10siwur 148 9 of of IN cord-269867-k10siwur 148 10 the the DT cord-269867-k10siwur 148 11 hybrids hybrid NNS cord-269867-k10siwur 148 12 containing contain VBG cord-269867-k10siwur 148 13 one one CD cord-269867-k10siwur 148 14 copy copy NN cord-269867-k10siwur 148 15 of of IN cord-269867-k10siwur 148 16 TGEV TGEV NNP cord-269867-k10siwur 148 17 - - HYPH cord-269867-k10siwur 148 18 A A NNP cord-269867-k10siwur 148 19 was be VBD cord-269867-k10siwur 148 20 detected detect VBN cord-269867-k10siwur 148 21 with with IN cord-269867-k10siwur 148 22 1AF10 1af10 CD cord-269867-k10siwur 148 23 mAb mAb NNS cord-269867-k10siwur 148 24 ; ; , cord-269867-k10siwur 148 25 ( ( -LRB- cord-269867-k10siwur 148 26 2 2 LS cord-269867-k10siwur 148 27 ) ) -RRB- cord-269867-k10siwur 148 28 no no DT cord-269867-k10siwur 148 29 proteolysis proteolysis NN cord-269867-k10siwur 148 30 happened happen VBD cord-269867-k10siwur 148 31 with with IN cord-269867-k10siwur 148 32 ClpG ClpG NNP cord-269867-k10siwur 148 33 alone alone RB cord-269867-k10siwur 148 34 or or CC cord-269867-k10siwur 148 35 in in IN cord-269867-k10siwur 148 36 association association NN cord-269867-k10siwur 148 37 with with IN cord-269867-k10siwur 148 38 TGEV TGEV NNP cord-269867-k10siwur 148 39 - - HYPH cord-269867-k10siwur 148 40 C C NNP cord-269867-k10siwur 148 41 ; ; , cord-269867-k10siwur 148 42 ( ( -LRB- cord-269867-k10siwur 148 43 3 3 LS cord-269867-k10siwur 148 44 ) ) -RRB- cord-269867-k10siwur 148 45 ClpG202-XC clpg202-xc JJ cord-269867-k10siwur 148 46 chimera chimera NN cord-269867-k10siwur 148 47 that that WDT cord-269867-k10siwur 148 48 differs differ VBZ cord-269867-k10siwur 148 49 from from IN cord-269867-k10siwur 148 50 ClpG202-AC ClpG202-AC NNP cord-269867-k10siwur 148 51 in in IN cord-269867-k10siwur 148 52 only only RB cord-269867-k10siwur 148 53 a a DT cord-269867-k10siwur 148 54 TGEV TGEV NNP cord-269867-k10siwur 148 55 - - HYPH cord-269867-k10siwur 148 56 A a DT cord-269867-k10siwur 148 57 sequence sequence NN cord-269867-k10siwur 148 58 in in IN cord-269867-k10siwur 148 59 reverse reverse JJ cord-269867-k10siwur 148 60 orientation orientation NN cord-269867-k10siwur 148 61 , , , cord-269867-k10siwur 148 62 was be VBD cord-269867-k10siwur 148 63 not not RB cord-269867-k10siwur 148 64 cleaved cleave VBN cord-269867-k10siwur 148 65 ; ; : cord-269867-k10siwur 148 66 ( ( -LRB- cord-269867-k10siwur 148 67 4 4 LS cord-269867-k10siwur 148 68 ) ) -RRB- cord-269867-k10siwur 148 69 the the DT cord-269867-k10siwur 148 70 immunoblot immunoblot NNP cord-269867-k10siwur 148 71 patterns pattern NNS cord-269867-k10siwur 148 72 of of IN cord-269867-k10siwur 148 73 ClpG1/203-CA ClpG1/203-CA NNP cord-269867-k10siwur 148 74 , , , cord-269867-k10siwur 148 75 carrying carry VBG cord-269867-k10siwur 148 76 two two CD cord-269867-k10siwur 148 77 copies copy NNS cord-269867-k10siwur 148 78 of of IN cord-269867-k10siwur 148 79 TGEV TGEV NNP cord-269867-k10siwur 148 80 - - HYPH cord-269867-k10siwur 148 81 A A NNP cord-269867-k10siwur 148 82 , , , cord-269867-k10siwur 148 83 are be VBP cord-269867-k10siwur 148 84 consistent consistent JJ cord-269867-k10siwur 148 85 with with IN cord-269867-k10siwur 148 86 the the DT cord-269867-k10siwur 148 87 presence presence NN cord-269867-k10siwur 148 88 of of IN cord-269867-k10siwur 148 89 a a DT cord-269867-k10siwur 148 90 cleavage cleavage NN cord-269867-k10siwur 148 91 site site NN cord-269867-k10siwur 148 92 within within IN cord-269867-k10siwur 148 93 each each DT cord-269867-k10siwur 148 94 copy copy NN cord-269867-k10siwur 148 95 since since IN cord-269867-k10siwur 148 96 four four CD cord-269867-k10siwur 148 97 fragments fragment NNS cord-269867-k10siwur 148 98 were be VBD cord-269867-k10siwur 148 99 visualized visualize VBN cord-269867-k10siwur 148 100 . . . cord-269867-k10siwur 149 1 This this DT cord-269867-k10siwur 149 2 implies imply VBZ cord-269867-k10siwur 149 3 that that IN cord-269867-k10siwur 149 4 TGEV TGEV NNP cord-269867-k10siwur 149 5 - - HYPH cord-269867-k10siwur 149 6 A a NN cord-269867-k10siwur 149 7 fused fuse VBN cord-269867-k10siwur 149 8 to to IN cord-269867-k10siwur 149 9 the the DT cord-269867-k10siwur 149 10 N n CD cord-269867-k10siwur 149 11 terminus terminus NN cord-269867-k10siwur 149 12 of of IN cord-269867-k10siwur 149 13 ClpG ClpG NNP cord-269867-k10siwur 149 14 was be VBD cord-269867-k10siwur 149 15 cleaved cleave VBN cord-269867-k10siwur 149 16 in in IN cord-269867-k10siwur 149 17 a a DT cord-269867-k10siwur 149 18 fashion fashion NN cord-269867-k10siwur 149 19 similar similar JJ cord-269867-k10siwur 149 20 to to IN cord-269867-k10siwur 149 21 TGEV TGEV NNP cord-269867-k10siwur 149 22 - - HYPH cord-269867-k10siwur 149 23 A a NN cord-269867-k10siwur 149 24 in in IN cord-269867-k10siwur 149 25 the the DT cord-269867-k10siwur 149 26 C c NN cord-269867-k10siwur 149 27 - - HYPH cord-269867-k10siwur 149 28 terminal terminal NN cord-269867-k10siwur 149 29 part part NN cord-269867-k10siwur 149 30 of of IN cord-269867-k10siwur 149 31 ClpG. ClpG. . cord-269867-k10siwur 150 1 In in IN cord-269867-k10siwur 150 2 this this DT cord-269867-k10siwur 150 3 case case NN cord-269867-k10siwur 150 4 , , , cord-269867-k10siwur 150 5 proteolytic proteolytic JJ cord-269867-k10siwur 150 6 cleavage cleavage NN cord-269867-k10siwur 150 7 would would MD cord-269867-k10siwur 150 8 result result VB cord-269867-k10siwur 150 9 in in IN cord-269867-k10siwur 150 10 a a DT cord-269867-k10siwur 150 11 N n CD cord-269867-k10siwur 150 12 - - HYPH cord-269867-k10siwur 150 13 terminal terminal NN cord-269867-k10siwur 150 14 fragment fragment NN cord-269867-k10siwur 150 15 too too RB cord-269867-k10siwur 150 16 small small JJ cord-269867-k10siwur 150 17 ( ( -LRB- cord-269867-k10siwur 150 18 < < XX cord-269867-k10siwur 150 19 20 20 CD cord-269867-k10siwur 150 20 aa aa NNP cord-269867-k10siwur 150 21 ) ) -RRB- cord-269867-k10siwur 150 22 to to TO cord-269867-k10siwur 150 23 be be VB cord-269867-k10siwur 150 24 detected detect VBN cord-269867-k10siwur 150 25 , , , cord-269867-k10siwur 150 26 that that IN cord-269867-k10siwur 150 27 explaining explain VBG cord-269867-k10siwur 150 28 why why WRB cord-269867-k10siwur 150 29 no no DT cord-269867-k10siwur 150 30 truncated truncated JJ cord-269867-k10siwur 150 31 product product NN cord-269867-k10siwur 150 32 was be VBD cord-269867-k10siwur 150 33 visualized visualize VBN cord-269867-k10siwur 150 34 from from IN cord-269867-k10siwur 150 35 ClpG1-CA ClpG1-CA NNP cord-269867-k10siwur 150 36 or or CC cord-269867-k10siwur 150 37 ClpG1-AC clpg1-ac NN cord-269867-k10siwur 151 1 ( ( -LRB- cord-269867-k10siwur 151 2 Fig Fig NNP cord-269867-k10siwur 151 3 . . NNP cord-269867-k10siwur 151 4 4 4 CD cord-269867-k10siwur 151 5 ) ) -RRB- cord-269867-k10siwur 151 6 . . . cord-269867-k10siwur 152 1 To to TO cord-269867-k10siwur 152 2 probe probe VB cord-269867-k10siwur 152 3 more more RBR cord-269867-k10siwur 152 4 precisely precisely RB cord-269867-k10siwur 152 5 the the DT cord-269867-k10siwur 152 6 N N NNP cord-269867-k10siwur 152 7 - - HYPH cord-269867-k10siwur 152 8 and and CC cord-269867-k10siwur 152 9 C c NN cord-269867-k10siwur 152 10 - - HYPH cord-269867-k10siwur 152 11 terminal terminal NN cord-269867-k10siwur 152 12 parts part NNS cord-269867-k10siwur 152 13 of of IN cord-269867-k10siwur 152 14 the the DT cord-269867-k10siwur 152 15 truncated truncated JJ cord-269867-k10siwur 152 16 proteins protein NNS cord-269867-k10siwur 152 17 generated generate VBN cord-269867-k10siwur 152 18 by by IN cord-269867-k10siwur 152 19 ClpG203-A clpg203-a NN cord-269867-k10siwur 152 20 , , , cord-269867-k10siwur 152 21 ClpG203-CA ClpG203-CA NNP cord-269867-k10siwur 152 22 and and CC cord-269867-k10siwur 152 23 ClpG202-AC ClpG202-AC NNP cord-269867-k10siwur 152 24 we -PRON- PRP cord-269867-k10siwur 152 25 used use VBD cord-269867-k10siwur 152 26 two two CD cord-269867-k10siwur 152 27 additional additional JJ cord-269867-k10siwur 152 28 specific specific JJ cord-269867-k10siwur 152 29 pAb pab NN cord-269867-k10siwur 152 30 , , , cord-269867-k10siwur 152 31 anti anti JJ cord-269867-k10siwur 152 32 - - JJ cord-269867-k10siwur 152 33 G15Q g15q JJ cord-269867-k10siwur 152 34 and and CC cord-269867-k10siwur 152 35 anti anti NNS cord-269867-k10siwur 152 36 - - JJ cord-269867-k10siwur 152 37 T15P t15p JJ cord-269867-k10siwur 152 38 , , , cord-269867-k10siwur 152 39 that that WDT cord-269867-k10siwur 152 40 recognize recognize VBP cord-269867-k10siwur 152 41 the the DT cord-269867-k10siwur 152 42 peptides peptide NNS cord-269867-k10siwur 152 43 G15Q G15Q NNP cord-269867-k10siwur 153 1 ( ( -LRB- cord-269867-k10siwur 153 2 aa aa NNP cord-269867-k10siwur 153 3 185 185 CD cord-269867-k10siwur 153 4 - - SYM cord-269867-k10siwur 153 5 199 199 CD cord-269867-k10siwur 153 6 ) ) -RRB- cord-269867-k10siwur 154 1 and and CC cord-269867-k10siwur 154 2 T15P T15P NNP cord-269867-k10siwur 154 3 ( ( -LRB- cord-269867-k10siwur 154 4 aa aa NNP cord-269867-k10siwur 154 5 235 235 CD cord-269867-k10siwur 154 6 - - HYPH cord-269867-k10siwur 154 7 249 249 CD cord-269867-k10siwur 154 8 ) ) -RRB- cord-269867-k10siwur 154 9 in in IN cord-269867-k10siwur 154 10 ClpG ClpG NNP cord-269867-k10siwur 154 11 , , , cord-269867-k10siwur 154 12 respectively respectively RB cord-269867-k10siwur 155 1 ; ; : cord-269867-k10siwur 155 2 insertions insertion NNS cord-269867-k10siwur 155 3 in in IN cord-269867-k10siwur 155 4 G15Q g15q NN cord-269867-k10siwur 155 5 sequence sequence NN cord-269867-k10siwur 155 6 abolished abolish VBD cord-269867-k10siwur 155 7 the the DT cord-269867-k10siwur 155 8 binding binding NN cord-269867-k10siwur 155 9 of of IN cord-269867-k10siwur 155 10 anti anti JJ cord-269867-k10siwur 155 11 - - JJ cord-269867-k10siwur 155 12 G15Q g15q JJ cord-269867-k10siwur 155 13 ( ( -LRB- cord-269867-k10siwur 155 14 Fig Fig NNP cord-269867-k10siwur 155 15 . . NNP cord-269867-k10siwur 155 16 4d 4d NNP cord-269867-k10siwur 155 17 , , , cord-269867-k10siwur 155 18 mutants mutant NNS cord-269867-k10siwur 155 19 ClpG192-C ClpG192-C NNP cord-269867-k10siwur 155 20 to to IN cord-269867-k10siwur 155 21 ClpG197-C ClpG197-C NNP cord-269867-k10siwur 155 22 ) ) -RRB- cord-269867-k10siwur 155 23 . . . cord-269867-k10siwur 156 1 In in IN cord-269867-k10siwur 156 2 these these DT cord-269867-k10siwur 156 3 constructs construct NNS cord-269867-k10siwur 156 4 , , , cord-269867-k10siwur 156 5 G15Q G15Q NNP cord-269867-k10siwur 156 6 is be VBZ cord-269867-k10siwur 156 7 placed place VBN cord-269867-k10siwur 156 8 10 10 CD cord-269867-k10siwur 156 9 aa aa NN cord-269867-k10siwur 156 10 upstream upstream RB cord-269867-k10siwur 156 11 from from IN cord-269867-k10siwur 156 12 the the DT cord-269867-k10siwur 156 13 ClpG::TGEV ClpG::TGEV NNP cord-269867-k10siwur 156 14 epitope epitope NN cord-269867-k10siwur 156 15 fusion fusion NN cord-269867-k10siwur 156 16 junction junction NN cord-269867-k10siwur 156 17 and and CC cord-269867-k10siwur 156 18 T15P T15P NNP cord-269867-k10siwur 156 19 20 20 CD cord-269867-k10siwur 156 20 to to TO cord-269867-k10siwur 156 21 30 30 CD cord-269867-k10siwur 156 22 aa aa NNP cord-269867-k10siwur 156 23 downstream downstream RB cord-269867-k10siwur 156 24 . . . cord-269867-k10siwur 157 1 Western western JJ cord-269867-k10siwur 157 2 immunoblots immunoblot NNS cord-269867-k10siwur 157 3 indicated indicate VBD cord-269867-k10siwur 157 4 that that IN cord-269867-k10siwur 157 5 the the DT cord-269867-k10siwur 157 6 intact intact JJ cord-269867-k10siwur 157 7 ClpG203-CA ClpG203-CA NNP cord-269867-k10siwur 157 8 and and CC cord-269867-k10siwur 157 9 CIpG202-AC CIpG202-AC NNP cord-269867-k10siwur 157 10 molecules molecule NNS cord-269867-k10siwur 157 11 reacted react VBD cord-269867-k10siwur 157 12 with with IN cord-269867-k10siwur 157 13 both both CC cord-269867-k10siwur 157 14 anti anti JJ cord-269867-k10siwur 157 15 - - JJ cord-269867-k10siwur 157 16 G15Q g15q JJ cord-269867-k10siwur 157 17 and and CC cord-269867-k10siwur 157 18 anti anti JJ cord-269867-k10siwur 157 19 - - JJ cord-269867-k10siwur 157 20 T15P t15p JJ cord-269867-k10siwur 157 21 ; ; : cord-269867-k10siwur 157 22 in in IN cord-269867-k10siwur 157 23 CIpG203-A cipg203-a NN cord-269867-k10siwur 157 24 the the DT cord-269867-k10siwur 157 25 intact intact JJ cord-269867-k10siwur 157 26 molecule molecule NN cord-269867-k10siwur 157 27 is be VBZ cord-269867-k10siwur 157 28 undetectable undetectable JJ cord-269867-k10siwur 157 29 because because IN cord-269867-k10siwur 157 30 too too RB cord-269867-k10siwur 157 31 low low JJ cord-269867-k10siwur 157 32 in in IN cord-269867-k10siwur 157 33 amount amount NN cord-269867-k10siwur 157 34 ( ( -LRB- cord-269867-k10siwur 157 35 Fig Fig NNP cord-269867-k10siwur 157 36 . . NNP cord-269867-k10siwur 157 37 4d 4d NNP cord-269867-k10siwur 157 38 , , , cord-269867-k10siwur 157 39 e e LS cord-269867-k10siwur 157 40 ) ) -RRB- cord-269867-k10siwur 157 41 . . . cord-269867-k10siwur 158 1 By by IN cord-269867-k10siwur 158 2 contrast contrast NN cord-269867-k10siwur 158 3 , , , cord-269867-k10siwur 158 4 their -PRON- PRP$ cord-269867-k10siwur 158 5 degraded degrade VBN cord-269867-k10siwur 158 6 forms form NNS cord-269867-k10siwur 158 7 were be VBD cord-269867-k10siwur 158 8 probed probe VBN cord-269867-k10siwur 158 9 by by IN cord-269867-k10siwur 158 10 anti anti JJ cord-269867-k10siwur 158 11 - - JJ cord-269867-k10siwur 158 12 G15Q g15q JJ cord-269867-k10siwur 158 13 but but CC cord-269867-k10siwur 158 14 not not RB cord-269867-k10siwur 158 15 by by IN cord-269867-k10siwur 158 16 anti anti JJ cord-269867-k10siwur 158 17 - - JJ cord-269867-k10siwur 158 18 T15P t15p JJ cord-269867-k10siwur 158 19 ( ( -LRB- cord-269867-k10siwur 158 20 Fig Fig NNP cord-269867-k10siwur 158 21 . . . cord-269867-k10siwur 158 22 4d 4d CD cord-269867-k10siwur 158 23 ) ) -RRB- cord-269867-k10siwur 158 24 . . . cord-269867-k10siwur 159 1 Together together RB cord-269867-k10siwur 159 2 with with IN cord-269867-k10siwur 159 3 the the DT cord-269867-k10siwur 159 4 facts fact NNS cord-269867-k10siwur 159 5 that that WDT cord-269867-k10siwur 159 6 the the DT cord-269867-k10siwur 159 7 cleaved cleaved JJ cord-269867-k10siwur 159 8 form form NN cord-269867-k10siwur 159 9 of of IN cord-269867-k10siwur 159 10 ClpG203-CA ClpG203-CA NNP cord-269867-k10siwur 159 11 was be VBD cord-269867-k10siwur 159 12 detected detect VBN cord-269867-k10siwur 159 13 with with IN cord-269867-k10siwur 159 14 3b.5mAb 3b.5mAb NNP cord-269867-k10siwur 159 15 ( ( -LRB- cord-269867-k10siwur 159 16 Fig fig NN cord-269867-k10siwur 159 17 . . . cord-269867-k10siwur 159 18 4b 4b CD cord-269867-k10siwur 159 19 ) ) -RRB- cord-269867-k10siwur 159 20 but but CC cord-269867-k10siwur 159 21 not not RB cord-269867-k10siwur 159 22 with with IN cord-269867-k10siwur 159 23 1AF10mAb 1af10mab CD cord-269867-k10siwur 159 24 ( ( -LRB- cord-269867-k10siwur 159 25 Fig Fig NNP cord-269867-k10siwur 159 26 . . NNP cord-269867-k10siwur 159 27 4c 4c CD cord-269867-k10siwur 159 28 ) ) -RRB- cord-269867-k10siwur 159 29 , , , cord-269867-k10siwur 159 30 and and CC cord-269867-k10siwur 159 31 that that IN cord-269867-k10siwur 159 32 the the DT cord-269867-k10siwur 159 33 truncated truncated JJ cord-269867-k10siwur 159 34 product product NN cord-269867-k10siwur 159 35 of of IN cord-269867-k10siwur 159 36 CIpG202-AC cipg202-ac NN cord-269867-k10siwur 159 37 did do VBD cord-269867-k10siwur 159 38 not not RB cord-269867-k10siwur 159 39 react react VB cord-269867-k10siwur 159 40 with with IN cord-269867-k10siwur 159 41 any any DT cord-269867-k10siwur 159 42 of of IN cord-269867-k10siwur 159 43 the the DT cord-269867-k10siwur 159 44 two two CD cord-269867-k10siwur 159 45 mAb mAb NNS cord-269867-k10siwur 159 46 ( ( -LRB- cord-269867-k10siwur 159 47 Fig Fig NNP cord-269867-k10siwur 159 48 . . . cord-269867-k10siwur 159 49 4b 4b CD cord-269867-k10siwur 159 50 , , , cord-269867-k10siwur 159 51 c c NNP cord-269867-k10siwur 159 52 ) ) -RRB- cord-269867-k10siwur 159 53 , , , cord-269867-k10siwur 159 54 these these DT cord-269867-k10siwur 159 55 data datum NNS cord-269867-k10siwur 159 56 suggest suggest VBP cord-269867-k10siwur 159 57 that that IN cord-269867-k10siwur 159 58 protease protease NN cord-269867-k10siwur 159 59 cleavage cleavage NN cord-269867-k10siwur 159 60 occurs occur VBZ cord-269867-k10siwur 159 61 within within IN cord-269867-k10siwur 159 62 TGEV TGEV NNP cord-269867-k10siwur 159 63 - - HYPH cord-269867-k10siwur 159 64 A. a. NN cord-269867-k10siwur 160 1 Furthermore furthermore RB cord-269867-k10siwur 160 2 , , , cord-269867-k10siwur 160 3 the the DT cord-269867-k10siwur 160 4 uncleaved uncleaved JJ cord-269867-k10siwur 160 5 form form NN cord-269867-k10siwur 160 6 of of IN cord-269867-k10siwur 160 7 Clp Clp NNP cord-269867-k10siwur 160 8 - - HYPH cord-269867-k10siwur 160 9 G203-CA G203-CA NNP cord-269867-k10siwur 160 10 was be VBD cord-269867-k10siwur 160 11 substantially substantially RB cord-269867-k10siwur 160 12 more more RBR cord-269867-k10siwur 160 13 abundant abundant JJ cord-269867-k10siwur 160 14 than than IN cord-269867-k10siwur 160 15 its -PRON- PRP$ cord-269867-k10siwur 160 16 truncated truncated JJ cord-269867-k10siwur 160 17 form form NN cord-269867-k10siwur 160 18 ( ( -LRB- cord-269867-k10siwur 160 19 Fig fig NN cord-269867-k10siwur 160 20 . . . cord-269867-k10siwur 160 21 4a 4a CD cord-269867-k10siwur 160 22 ) ) -RRB- cord-269867-k10siwur 160 23 . . . cord-269867-k10siwur 161 1 In in IN cord-269867-k10siwur 161 2 contrast contrast NN cord-269867-k10siwur 161 3 , , , cord-269867-k10siwur 161 4 CIpG203-A cipg203-a NN cord-269867-k10siwur 161 5 and and CC cord-269867-k10siwur 161 6 ClpG202-AC ClpG202-AC NNP cord-269867-k10siwur 161 7 showed show VBD cord-269867-k10siwur 161 8 a a DT cord-269867-k10siwur 161 9 prominence prominence NN cord-269867-k10siwur 161 10 in in IN cord-269867-k10siwur 161 11 amount amount NN cord-269867-k10siwur 161 12 of of IN cord-269867-k10siwur 161 13 the the DT cord-269867-k10siwur 161 14 cleaved cleaved JJ cord-269867-k10siwur 161 15 products product NNS cord-269867-k10siwur 161 16 , , , cord-269867-k10siwur 161 17 indicating indicate VBG cord-269867-k10siwur 161 18 that that IN cord-269867-k10siwur 161 19 C::A c::a JJ cord-269867-k10siwur 161 20 fusion fusion NN cord-269867-k10siwur 161 21 , , , cord-269867-k10siwur 161 22 rather rather RB cord-269867-k10siwur 161 23 than than IN cord-269867-k10siwur 161 24 A::C a::c JJ cord-269867-k10siwur 161 25 fusion fusion NN cord-269867-k10siwur 161 26 , , , cord-269867-k10siwur 161 27 significantly significantly RB cord-269867-k10siwur 161 28 reduced reduce VBN cord-269867-k10siwur 161 29 cleavage cleavage NN cord-269867-k10siwur 161 30 process process NN cord-269867-k10siwur 161 31 , , , cord-269867-k10siwur 161 32 probably probably RB cord-269867-k10siwur 161 33 due due IN cord-269867-k10siwur 161 34 to to IN cord-269867-k10siwur 161 35 the the DT cord-269867-k10siwur 161 36 protective protective JJ cord-269867-k10siwur 161 37 placement placement NN cord-269867-k10siwur 161 38 of of IN cord-269867-k10siwur 161 39 the the DT cord-269867-k10siwur 161 40 hydrophobic hydrophobic JJ cord-269867-k10siwur 161 41 TGEV TGEV NNP cord-269867-k10siwur 161 42 - - HYPH cord-269867-k10siwur 161 43 C C NNP cord-269867-k10siwur 161 44 motif motif NN cord-269867-k10siwur 161 45 at at IN cord-269867-k10siwur 161 46 the the DT cord-269867-k10siwur 161 47 N n CD cord-269867-k10siwur 161 48 - - HYPH cord-269867-k10siwur 161 49 terminal terminal JJ cord-269867-k10siwur 161 50 end end NN cord-269867-k10siwur 161 51 of of IN cord-269867-k10siwur 162 1 TGEV TGEV NNP cord-269867-k10siwur 162 2 - - HYPH cord-269867-k10siwur 162 3 A. A. NNP cord-269867-k10siwur 162 4 Therefore therefore RB cord-269867-k10siwur 162 5 , , , cord-269867-k10siwur 162 6 we -PRON- PRP cord-269867-k10siwur 162 7 speculate speculate VBP cord-269867-k10siwur 162 8 that that IN cord-269867-k10siwur 162 9 the the DT cord-269867-k10siwur 162 10 site site NN cord-269867-k10siwur 162 11 of of IN cord-269867-k10siwur 162 12 cleavage cleavage NN cord-269867-k10siwur 162 13 must must MD cord-269867-k10siwur 162 14 be be VB cord-269867-k10siwur 162 15 N n CD cord-269867-k10siwur 162 16 - - HYPH cord-269867-k10siwur 162 17 terminally terminally RB cord-269867-k10siwur 162 18 located locate VBN cord-269867-k10siwur 162 19 with with IN cord-269867-k10siwur 162 20 respect respect NN cord-269867-k10siwur 162 21 to to IN cord-269867-k10siwur 162 22 the the DT cord-269867-k10siwur 162 23 TGEV TGEV NNP cord-269867-k10siwur 162 24 - - HYPH cord-269867-k10siwur 162 25 A a DT cord-269867-k10siwur 162 26 sequence sequence NN cord-269867-k10siwur 162 27 . . . cord-269867-k10siwur 163 1 Supporting support VBG cord-269867-k10siwur 163 2 this this DT cord-269867-k10siwur 163 3 hypothesis hypothesis NN cord-269867-k10siwur 163 4 , , , cord-269867-k10siwur 163 5 the the DT cord-269867-k10siwur 163 6 two two CD cord-269867-k10siwur 163 7 first first JJ cord-269867-k10siwur 163 8 aa aa NNP cord-269867-k10siwur 163 9 residues residue NNS cord-269867-k10siwur 163 10 of of IN cord-269867-k10siwur 163 11 TGEV TGEV NNP cord-269867-k10siwur 163 12 - - HYPH cord-269867-k10siwur 163 13 A A NNP cord-269867-k10siwur 163 14 consist consist NN cord-269867-k10siwur 163 15 of of IN cord-269867-k10siwur 163 16 lysine lysine NN cord-269867-k10siwur 163 17 ( ( -LRB- cord-269867-k10siwur 163 18 K K NNP cord-269867-k10siwur 163 19 ) ) -RRB- cord-269867-k10siwur 163 20 and and CC cord-269867-k10siwur 163 21 arginine arginine NN cord-269867-k10siwur 163 22 ( ( -LRB- cord-269867-k10siwur 163 23 R r NN cord-269867-k10siwur 163 24 ) ) -RRB- cord-269867-k10siwur 163 25 which which WDT cord-269867-k10siwur 163 26 are be VBP cord-269867-k10siwur 163 27 often often RB cord-269867-k10siwur 163 28 involved involve VBN cord-269867-k10siwur 163 29 in in IN cord-269867-k10siwur 163 30 the the DT cord-269867-k10siwur 163 31 proteolytic proteolytic JJ cord-269867-k10siwur 163 32 maturation maturation NN cord-269867-k10siwur 163 33 of of IN cord-269867-k10siwur 163 34 viral viral JJ cord-269867-k10siwur 163 35 envelope envelope NN cord-269867-k10siwur 163 36 glycoproteins glycoprotein NNS cord-269867-k10siwur 163 37 ( ( -LRB- cord-269867-k10siwur 163 38 Moulard Moulard NNP cord-269867-k10siwur 163 39 et et FW cord-269867-k10siwur 163 40 al al NNP cord-269867-k10siwur 163 41 . . NNP cord-269867-k10siwur 163 42 , , , cord-269867-k10siwur 163 43 1995 1995 CD cord-269867-k10siwur 163 44 ) ) -RRB- cord-269867-k10siwur 163 45 . . . cord-269867-k10siwur 164 1 assistance assistance NN cord-269867-k10siwur 164 2 , , , cord-269867-k10siwur 164 3 S. S. NNP cord-269867-k10siwur 164 4 Dutilloy Dutilloy NNP cord-269867-k10siwur 164 5 for for IN cord-269867-k10siwur 164 6 secretarial secretarial JJ cord-269867-k10siwur 164 7 assistance assistance NN cord-269867-k10siwur 164 8 , , , cord-269867-k10siwur 164 9 Dr. Dr. NNP cord-269867-k10siwur 164 10 L. L. NNP cord-269867-k10siwur 164 11 Enjuanes Enjuanes NNP cord-269867-k10siwur 164 12 and and CC cord-269867-k10siwur 164 13 Dr. Dr. NNP cord-269867-k10siwur 164 14 H. H. NNP cord-269867-k10siwur 164 15 Laude Laude NNP cord-269867-k10siwur 164 16 for for IN cord-269867-k10siwur 164 17 providing provide VBG cord-269867-k10siwur 164 18 mAb mab NN cord-269867-k10siwur 164 19 1AF10 1AF10 NNS cord-269867-k10siwur 164 20 and and CC cord-269867-k10siwur 164 21 mAb mab NN cord-269867-k10siwur 164 22 3b.5 3b.5 CD cord-269867-k10siwur 164 23 , , , cord-269867-k10siwur 164 24 respectively respectively RB cord-269867-k10siwur 164 25 . . . cord-269867-k10siwur 165 1 This this DT cord-269867-k10siwur 165 2 work work NN cord-269867-k10siwur 165 3 was be VBD cord-269867-k10siwur 165 4 supported support VBN cord-269867-k10siwur 165 5 by by IN cord-269867-k10siwur 165 6 grants grant NNS cord-269867-k10siwur 165 7 AGRE-0008-C AGRE-0008-C NNP cord-269867-k10siwur 165 8 from from IN cord-269867-k10siwur 165 9 European European NNP cord-269867-k10siwur 165 10 Economic Economic NNP cord-269867-k10siwur 165 11 Community Community NNP cord-269867-k10siwur 165 12 ( ( -LRB- cord-269867-k10siwur 165 13 ECLAIR ECLAIR NNP cord-269867-k10siwur 165 14 program program NN cord-269867-k10siwur 165 15 ) ) -RRB- cord-269867-k10siwur 165 16 . . . cord-269867-k10siwur 166 1 ( ( -LRB- cord-269867-k10siwur 166 2 1 1 CD cord-269867-k10siwur 166 3 ) ) -RRB- cord-269867-k10siwur 167 1 Three three CD cord-269867-k10siwur 167 2 nonpermissive nonpermissive JJ cord-269867-k10siwur 167 3 sites site NNS cord-269867-k10siwur 167 4 ( ( -LRB- cord-269867-k10siwur 167 5 aa aa NNP cord-269867-k10siwur 167 6 182 182 CD cord-269867-k10siwur 167 7 - - SYM cord-269867-k10siwur 167 8 183 183 CD cord-269867-k10siwur 167 9 , , , cord-269867-k10siwur 167 10 190 190 CD cord-269867-k10siwur 167 11 - - SYM cord-269867-k10siwur 167 12 191 191 CD cord-269867-k10siwur 167 13 and and CC cord-269867-k10siwur 167 14 191 191 CD cord-269867-k10siwur 167 15 - - SYM cord-269867-k10siwur 167 16 192 192 CD cord-269867-k10siwur 167 17 ) ) -RRB- cord-269867-k10siwur 167 18 , , , cord-269867-k10siwur 167 19 six six CD cord-269867-k10siwur 167 20 partially partially RB cord-269867-k10siwur 167 21 permissive permissive JJ cord-269867-k10siwur 167 22 sites site NNS cord-269867-k10siwur 167 23 ( ( -LRB- cord-269867-k10siwur 167 24 aa aa NNP cord-269867-k10siwur 167 25 192 192 CD cord-269867-k10siwur 167 26 to to IN cord-269867-k10siwur 167 27 197 197 CD cord-269867-k10siwur 167 28 ) ) -RRB- cord-269867-k10siwur 167 29 and and CC cord-269867-k10siwur 167 30 three three CD cord-269867-k10siwur 167 31 fully fully RB cord-269867-k10siwur 167 32 permissive permissive JJ cord-269867-k10siwur 167 33 sites site NNS cord-269867-k10siwur 167 34 ( ( -LRB- cord-269867-k10siwur 167 35 aa aa NNP cord-269867-k10siwur 167 36 -1/1 -1/1 NNP cord-269867-k10siwur 167 37 , , , cord-269867-k10siwur 167 38 202 202 CD cord-269867-k10siwur 167 39 - - SYM cord-269867-k10siwur 167 40 204 204 CD cord-269867-k10siwur 167 41 and and CC cord-269867-k10siwur 167 42 202 202 CD cord-269867-k10siwur 167 43 - - SYM cord-269867-k10siwur 167 44 218 218 CD cord-269867-k10siwur 167 45 ) ) -RRB- cord-269867-k10siwur 167 46 were be VBD cord-269867-k10siwur 167 47 identified identify VBN cord-269867-k10siwur 167 48 throughout throughout IN cord-269867-k10siwur 167 49 CIpG. cipg. XX cord-269867-k10siwur 168 1 The the DT cord-269867-k10siwur 168 2 latter latter JJ cord-269867-k10siwur 168 3 sites site NNS cord-269867-k10siwur 168 4 appeared appear VBD cord-269867-k10siwur 168 5 as as IN cord-269867-k10siwur 168 6 the the DT cord-269867-k10siwur 168 7 most most RBS cord-269867-k10siwur 168 8 versatile versatile JJ cord-269867-k10siwur 168 9 targets target NNS cord-269867-k10siwur 168 10 since since IN cord-269867-k10siwur 168 11 the the DT cord-269867-k10siwur 168 12 largest large JJS cord-269867-k10siwur 168 13 insertions insertion NNS cord-269867-k10siwur 168 14 consisting consist VBG cord-269867-k10siwur 168 15 of of IN cord-269867-k10siwur 168 16 26 26 CD cord-269867-k10siwur 169 1 ~ta ~ta NNP cord-269867-k10siwur 169 2 residues residue NNS cord-269867-k10siwur 169 3 did do VBD cord-269867-k10siwur 169 4 not not RB cord-269867-k10siwur 169 5 interfere interfere VB cord-269867-k10siwur 169 6 with with IN cord-269867-k10siwur 169 7 CS31A cs31a NN cord-269867-k10siwur 169 8 biogenesis biogenesis NN cord-269867-k10siwur 169 9 . . . cord-269867-k10siwur 170 1 Even even RB cord-269867-k10siwur 170 2 an an DT cord-269867-k10siwur 170 3 insert insert NN cord-269867-k10siwur 170 4 of of IN cord-269867-k10siwur 170 5 51 51 CD cord-269867-k10siwur 170 6 aa aa NNP cord-269867-k10siwur 170 7 long long JJ cord-269867-k10siwur 170 8 resulting resulting NN cord-269867-k10siwur 170 9 of of IN cord-269867-k10siwur 170 10 the the DT cord-269867-k10siwur 170 11 addition addition NN cord-269867-k10siwur 170 12 of of IN cord-269867-k10siwur 170 13 the the DT cord-269867-k10siwur 170 14 TGEV TGEV NNP cord-269867-k10siwur 170 15 - - HYPH cord-269867-k10siwur 170 16 C::TGEV C::TGEV NNP cord-269867-k10siwur 170 17 - - HYPH cord-269867-k10siwur 170 18 A a DT cord-269867-k10siwur 170 19 tandem tandem NN cord-269867-k10siwur 170 20 peptide peptide NN cord-269867-k10siwur 170 21 in in IN cord-269867-k10siwur 170 22 both both DT cord-269867-k10siwur 170 23 positions position NNS cord-269867-k10siwur 171 1 aa aa NNP cord-269867-k10siwur 171 2 -1/1 -1/1 NNP cord-269867-k10siwur 171 3 and and CC cord-269867-k10siwur 171 4 202 202 CD cord-269867-k10siwur 171 5 - - SYM cord-269867-k10siwur 171 6 218 218 CD cord-269867-k10siwur 171 7 was be VBD cord-269867-k10siwur 171 8 normally normally RB cord-269867-k10siwur 171 9 incorporated incorporate VBN cord-269867-k10siwur 171 10 in in IN cord-269867-k10siwur 171 11 CS31A cs31a NN cord-269867-k10siwur 171 12 polymer polymer NN cord-269867-k10siwur 171 13 . . . cord-269867-k10siwur 172 1 The the DT cord-269867-k10siwur 172 2 binding binding NN cord-269867-k10siwur 172 3 of of IN cord-269867-k10siwur 172 4 the the DT cord-269867-k10siwur 172 5 TGEV TGEV NNP cord-269867-k10siwur 172 6 - - HYPH cord-269867-k10siwur 172 7 specific specific JJ cord-269867-k10siwur 172 8 mAb mab NN cord-269867-k10siwur 172 9 with with IN cord-269867-k10siwur 172 10 hybrid hybrid JJ cord-269867-k10siwur 172 11 fibrillae fibrillae NNP cord-269867-k10siwur 172 12 indicates indicate VBZ cord-269867-k10siwur 172 13 that that IN cord-269867-k10siwur 172 14 the the DT cord-269867-k10siwur 172 15 TGEV TGEV NNP cord-269867-k10siwur 172 16 antigenic antigenic JJ cord-269867-k10siwur 172 17 determinants determinant NNS cord-269867-k10siwur 172 18 expressed express VBN cord-269867-k10siwur 172 19 at at IN cord-269867-k10siwur 172 20 any any DT cord-269867-k10siwur 172 21 of of IN cord-269867-k10siwur 172 22 the the DT cord-269867-k10siwur 172 23 permissive permissive JJ cord-269867-k10siwur 172 24 sites site NNS cord-269867-k10siwur 172 25 are be VBP cord-269867-k10siwur 172 26 exposed expose VBN cord-269867-k10siwur 172 27 on on IN cord-269867-k10siwur 172 28 both both DT cord-269867-k10siwur 172 29 ClpG ClpG NNP cord-269867-k10siwur 172 30 and and CC cord-269867-k10siwur 172 31 CS31A cs31a NN cord-269867-k10siwur 172 32 proteins protein NNS cord-269867-k10siwur 172 33 at at IN cord-269867-k10siwur 172 34 the the DT cord-269867-k10siwur 172 35 E. E. NNP cord-269867-k10siwur 172 36 coli coli VBZ cord-269867-k10siwur 172 37 cell cell NN cord-269867-k10siwur 172 38 - - HYPH cord-269867-k10siwur 172 39 surface surface NN cord-269867-k10siwur 172 40 . . . cord-269867-k10siwur 173 1 We -PRON- PRP cord-269867-k10siwur 173 2 show show VBP cord-269867-k10siwur 173 3 here here RB cord-269867-k10siwur 173 4 that that IN cord-269867-k10siwur 173 5 the the DT cord-269867-k10siwur 173 6 potential potential NN cord-269867-k10siwur 173 7 of of IN cord-269867-k10siwur 173 8 ClpG ClpG NNP cord-269867-k10siwur 173 9 as as IN cord-269867-k10siwur 173 10 a a DT cord-269867-k10siwur 173 11 carrier carrier NN cord-269867-k10siwur 173 12 for for IN cord-269867-k10siwur 173 13 foreign foreign JJ cord-269867-k10siwur 173 14 peptides peptide NNS cord-269867-k10siwur 173 15 may may MD cord-269867-k10siwur 173 16 be be VB cord-269867-k10siwur 173 17 influenced influence VBN cord-269867-k10siwur 173 18 by by IN cord-269867-k10siwur 173 19 the the DT cord-269867-k10siwur 173 20 general general NN cord-269867-k10siwur 173 21 predicted predict VBN cord-269867-k10siwur 173 22 properties property NNS cord-269867-k10siwur 173 23 of of IN cord-269867-k10siwur 173 24 the the DT cord-269867-k10siwur 173 25 local local JJ cord-269867-k10siwur 173 26 sequences sequence NNS cord-269867-k10siwur 173 27 . . . cord-269867-k10siwur 174 1 ( ( -LRB- cord-269867-k10siwur 174 2 2 2 LS cord-269867-k10siwur 174 3 ) ) -RRB- cord-269867-k10siwur 174 4 Unlike unlike IN cord-269867-k10siwur 174 5 TGEV TGEV NNP cord-269867-k10siwur 174 6 - - HYPH cord-269867-k10siwur 174 7 C C NNP cord-269867-k10siwur 174 8 , , , cord-269867-k10siwur 174 9 TGEV TGEV NNP cord-269867-k10siwur 174 10 - - HYPH cord-269867-k10siwur 174 11 A A NNP cord-269867-k10siwur 174 12 expressed express VBN cord-269867-k10siwur 174 13 at at IN cord-269867-k10siwur 174 14 any any DT cord-269867-k10siwur 174 15 permissive permissive JJ cord-269867-k10siwur 174 16 site site NN cord-269867-k10siwur 174 17 of of IN cord-269867-k10siwur 174 18 ClpG ClpG NNP cord-269867-k10siwur 174 19 rendered render VBD cord-269867-k10siwur 174 20 the the DT cord-269867-k10siwur 174 21 hybrid hybrid JJ cord-269867-k10siwur 174 22 proteins protein NNS cord-269867-k10siwur 174 23 partially partially RB cord-269867-k10siwur 174 24 susceptible susceptible JJ cord-269867-k10siwur 174 25 to to IN cord-269867-k10siwur 174 26 proteolytic proteolytic JJ cord-269867-k10siwur 174 27 degradation degradation NN cord-269867-k10siwur 174 28 , , , cord-269867-k10siwur 174 29 indicating indicate VBG cord-269867-k10siwur 174 30 that that IN cord-269867-k10siwur 174 31 it -PRON- PRP cord-269867-k10siwur 174 32 was be VBD cord-269867-k10siwur 174 33 influential influential JJ cord-269867-k10siwur 174 34 in in IN cord-269867-k10siwur 174 35 , , , cord-269867-k10siwur 174 36 but but CC cord-269867-k10siwur 174 37 not not RB cord-269867-k10siwur 174 38 critical critical JJ cord-269867-k10siwur 174 39 for for IN cord-269867-k10siwur 174 40 , , , cord-269867-k10siwur 174 41 fibrillae fibrillae NNP cord-269867-k10siwur 174 42 integrity integrity NN cord-269867-k10siwur 174 43 , , , cord-269867-k10siwur 174 44 presumably presumably RB cord-269867-k10siwur 174 45 because because IN cord-269867-k10siwur 174 46 providing provide VBG cord-269867-k10siwur 174 47 a a DT cord-269867-k10siwur 174 48 protease protease NN cord-269867-k10siwur 174 49 cleavage cleavage NN cord-269867-k10siwur 174 50 site site NN cord-269867-k10siwur 174 51 . . . cord-269867-k10siwur 175 1 ( ( -LRB- cord-269867-k10siwur 175 2 3 3 LS cord-269867-k10siwur 175 3 ) ) -RRB- cord-269867-k10siwur 175 4 CS31A CS31A NNP cord-269867-k10siwur 175 5 fibrillum fibrillum NNP cord-269867-k10siwur 175 6 appears appear VBZ cord-269867-k10siwur 175 7 as as IN cord-269867-k10siwur 175 8 a a DT cord-269867-k10siwur 175 9 potent potent JJ cord-269867-k10siwur 175 10 cell cell NN cord-269867-k10siwur 175 11 - - HYPH cord-269867-k10siwur 175 12 surface surface NN cord-269867-k10siwur 175 13 presenting present VBG cord-269867-k10siwur 175 14 vector vector NN cord-269867-k10siwur 175 15 for for IN cord-269867-k10siwur 175 16 TGEV TGEV NNP cord-269867-k10siwur 175 17 epitopes epitope NNS cord-269867-k10siwur 175 18 . . . cord-269867-k10siwur 176 1 Such such JJ cord-269867-k10siwur 176 2 hybrid hybrid JJ cord-269867-k10siwur 176 3 fibrillae fibrilla NNS cord-269867-k10siwur 176 4 might may MD cord-269867-k10siwur 176 5 be be VB cord-269867-k10siwur 176 6 valuable valuable JJ cord-269867-k10siwur 176 7 tools tool NNS cord-269867-k10siwur 176 8 in in IN cord-269867-k10siwur 176 9 the the DT cord-269867-k10siwur 176 10 development development NN cord-269867-k10siwur 176 11 as as IN cord-269867-k10siwur 176 12 components component NNS cord-269867-k10siwur 176 13 of of IN cord-269867-k10siwur 176 14 a a DT cord-269867-k10siwur 176 15 subunit subunit JJ cord-269867-k10siwur 176 16 vaccine vaccine NN cord-269867-k10siwur 176 17 . . . cord-269867-k10siwur 177 1 Whether whether IN cord-269867-k10siwur 177 2 these these DT cord-269867-k10siwur 177 3 hybrid hybrid JJ cord-269867-k10siwur 177 4 fibrillae fibrilla NNS cord-269867-k10siwur 177 5 induce induce VBP cord-269867-k10siwur 177 6 specific specific JJ cord-269867-k10siwur 177 7 Ab ab NN cord-269867-k10siwur 177 8 is be VBZ cord-269867-k10siwur 177 9 currently currently RB cord-269867-k10siwur 177 10 under under IN cord-269867-k10siwur 177 11 investigation investigation NN cord-269867-k10siwur 177 12 . . . cord-269867-k10siwur 178 1 The the DT cord-269867-k10siwur 178 2 ClpG ClpG NNP cord-269867-k10siwur 178 3 exposure exposure NN cord-269867-k10siwur 178 4 vector vector NN cord-269867-k10siwur 178 5 system system NN cord-269867-k10siwur 178 6 described describe VBN cord-269867-k10siwur 178 7 here here RB cord-269867-k10siwur 178 8 provides provide VBZ cord-269867-k10siwur 178 9 a a DT cord-269867-k10siwur 178 10 number number NN cord-269867-k10siwur 178 11 of of IN cord-269867-k10siwur 178 12 insertion insertion NN cord-269867-k10siwur 178 13 sites site NNS cord-269867-k10siwur 178 14 and and CC cord-269867-k10siwur 178 15 therefore therefore RB cord-269867-k10siwur 178 16 a a DT cord-269867-k10siwur 178 17 variety variety NN cord-269867-k10siwur 178 18 of of IN cord-269867-k10siwur 178 19 flanking flank VBG cord-269867-k10siwur 178 20 aa aa NNP cord-269867-k10siwur 178 21 sequences sequence NNS cord-269867-k10siwur 178 22 for for IN cord-269867-k10siwur 178 23 the the DT cord-269867-k10siwur 178 24 expression expression NN cord-269867-k10siwur 178 25 of of IN cord-269867-k10siwur 178 26 TGEV TGEV NNP cord-269867-k10siwur 178 27 epitopes epitope NNS cord-269867-k10siwur 178 28 . . . cord-269867-k10siwur 179 1 Consequently consequently RB cord-269867-k10siwur 179 2 , , , cord-269867-k10siwur 179 3 a a DT cord-269867-k10siwur 179 4 further further JJ cord-269867-k10siwur 179 5 study study NN cord-269867-k10siwur 179 6 of of IN cord-269867-k10siwur 179 7 the the DT cord-269867-k10siwur 179 8 immunogenicity immunogenicity NN cord-269867-k10siwur 179 9 of of IN cord-269867-k10siwur 179 10 the the DT cord-269867-k10siwur 179 11 TGEV TGEV NNP cord-269867-k10siwur 179 12 epitopes epitope NNS cord-269867-k10siwur 179 13 in in IN cord-269867-k10siwur 179 14 different different JJ cord-269867-k10siwur 179 15 contexts context NNS cord-269867-k10siwur 179 16 of of IN cord-269867-k10siwur 179 17 ClpG ClpG NNP cord-269867-k10siwur 179 18 will will MD cord-269867-k10siwur 179 19 improve improve VB cord-269867-k10siwur 179 20 our -PRON- PRP$ cord-269867-k10siwur 179 21 understanding understanding NN cord-269867-k10siwur 179 22 of of IN cord-269867-k10siwur 179 23 the the DT cord-269867-k10siwur 179 24 relationship relationship NN cord-269867-k10siwur 179 25 between between IN cord-269867-k10siwur 179 26 flanking flank VBG cord-269867-k10siwur 179 27 sequences sequence NNS cord-269867-k10siwur 179 28 and and CC cord-269867-k10siwur 179 29 the the DT cord-269867-k10siwur 179 30 immunogenicity immunogenicity NN cord-269867-k10siwur 179 31 of of IN cord-269867-k10siwur 179 32 the the DT cord-269867-k10siwur 179 33 epitopes epitope NNS cord-269867-k10siwur 179 34 . . . cord-269867-k10siwur 180 1 Improved improve VBN cord-269867-k10siwur 180 2 mimicry mimicry NN cord-269867-k10siwur 180 3 of of IN cord-269867-k10siwur 180 4 a a DT cord-269867-k10siwur 180 5 foot foot NN cord-269867-k10siwur 180 6 - - HYPH cord-269867-k10siwur 180 7 and and CC cord-269867-k10siwur 180 8 - - HYPH cord-269867-k10siwur 180 9 mouth mouth NN cord-269867-k10siwur 180 10 disease disease NN cord-269867-k10siwur 180 11 virus virus NN cord-269867-k10siwur 180 12 antigenic antigenic JJ cord-269867-k10siwur 180 13 site site NN cord-269867-k10siwur 180 14 by by IN cord-269867-k10siwur 180 15 a a DT cord-269867-k10siwur 180 16 viral viral JJ cord-269867-k10siwur 180 17 peptide peptide NN cord-269867-k10siwur 180 18 displayed display VBN cord-269867-k10siwur 180 19 on on IN cord-269867-k10siwur 180 20 fl fl JJ cord-269867-k10siwur 180 21 - - HYPH cord-269867-k10siwur 180 22 galactosidase galactosidase NN cord-269867-k10siwur 180 23 surface surface NN cord-269867-k10siwur 180 24 CS31A cs31a NN cord-269867-k10siwur 180 25 capsule capsule NN cord-269867-k10siwur 180 26 - - HYPH cord-269867-k10siwur 180 27 like like JJ cord-269867-k10siwur 180 28 antigen antigen NN cord-269867-k10siwur 180 29 as as IN cord-269867-k10siwur 180 30 an an DT cord-269867-k10siwur 180 31 exposure exposure NN cord-269867-k10siwur 180 32 vector vector NN cord-269867-k10siwur 180 33 for for IN cord-269867-k10siwur 180 34 heterologous heterologous JJ cord-269867-k10siwur 180 35 antigenic antigenic JJ cord-269867-k10siwur 180 36 determinants determinant NNS cord-269867-k10siwur 180 37 Synthesis Synthesis NNP cord-269867-k10siwur 180 38 of of IN cord-269867-k10siwur 180 39 fusion fusion NN cord-269867-k10siwur 180 40 proteins protein NNS cord-269867-k10siwur 180 41 with with IN cord-269867-k10siwur 180 42 multiple multiple JJ cord-269867-k10siwur 180 43 copies copy NNS cord-269867-k10siwur 180 44 of of IN cord-269867-k10siwur 180 45 an an DT cord-269867-k10siwur 180 46 antiigenic antiigenic JJ cord-269867-k10siwur 180 47 determinant determinant NN cord-269867-k10siwur 180 48 of of IN cord-269867-k10siwur 180 49 footand footand NN cord-269867-k10siwur 180 50 - - HYPH cord-269867-k10siwur 180 51 mouth mouth NN cord-269867-k10siwur 180 52 disease disease NN cord-269867-k10siwur 180 53 virus virus NN cord-269867-k10siwur 180 54 Permissive permissive JJ cord-269867-k10siwur 180 55 sites site NNS cord-269867-k10siwur 180 56 and and CC cord-269867-k10siwur 180 57 topology topology NN cord-269867-k10siwur 180 58 of of IN cord-269867-k10siwur 180 59 an an DT cord-269867-k10siwur 180 60 outer outer JJ cord-269867-k10siwur 180 61 membrane membrane NN cord-269867-k10siwur 180 62 protein protein NN cord-269867-k10siwur 180 63 with with IN cord-269867-k10siwur 180 64 a a DT cord-269867-k10siwur 180 65 reporter reporter NN cord-269867-k10siwur 180 66 epitope epitope NN cord-269867-k10siwur 180 67 Localization localization NN cord-269867-k10siwur 180 68 of of IN cord-269867-k10siwur 180 69 antigenic antigenic JJ cord-269867-k10siwur 180 70 sites site NNS cord-269867-k10siwur 180 71 of of IN cord-269867-k10siwur 180 72 the the DT cord-269867-k10siwur 180 73 E2 E2 NNP cord-269867-k10siwur 180 74 glycoprotein glycoprotein NN cord-269867-k10siwur 180 75 of of IN cord-269867-k10siwur 180 76 transmissible transmissible JJ cord-269867-k10siwur 180 77 gastroenteritis gastroenteritis NN cord-269867-k10siwur 180 78 coronavirus coronavirus NN cord-269867-k10siwur 181 1 Four four CD cord-269867-k10siwur 181 2 major major JJ cord-269867-k10siwur 181 3 antigenic antigenic JJ cord-269867-k10siwur 181 4 sites site NNS cord-269867-k10siwur 181 5 of of IN cord-269867-k10siwur 181 6 the the DT cord-269867-k10siwur 181 7 coronavirus coronavirus NN cord-269867-k10siwur 181 8 transmissible transmissible JJ cord-269867-k10siwur 181 9 gastroenteritis gastroenteritis NN cord-269867-k10siwur 181 10 virus virus NN cord-269867-k10siwur 181 11 are be VBP cord-269867-k10siwur 181 12 located locate VBN cord-269867-k10siwur 181 13 on on IN cord-269867-k10siwur 181 14 the the DT cord-269867-k10siwur 181 15 amino amino NN cord-269867-k10siwur 181 16 - - HYPH cord-269867-k10siwur 181 17 terminal terminal NN cord-269867-k10siwur 181 18 half half NN cord-269867-k10siwur 181 19 of of IN cord-269867-k10siwur 181 20 spike spike NN cord-269867-k10siwur 181 21 protein protein NN cord-269867-k10siwur 181 22 Site site NN cord-269867-k10siwur 181 23 - - HYPH cord-269867-k10siwur 181 24 directed direct VBN cord-269867-k10siwur 181 25 mutagenesis mutagenesis NN cord-269867-k10siwur 181 26 of of IN cord-269867-k10siwur 181 27 virtually virtually RB cord-269867-k10siwur 181 28 any any DT cord-269867-k10siwur 181 29 plasmid plasmid NN cord-269867-k10siwur 181 30 by by IN cord-269867-k10siwur 181 31 eliminating eliminate VBG cord-269867-k10siwur 181 32 a a DT cord-269867-k10siwur 181 33 unique unique JJ cord-269867-k10siwur 181 34 site site NN cord-269867-k10siwur 181 35 Permissible permissible JJ cord-269867-k10siwur 181 36 peptide peptide NN cord-269867-k10siwur 181 37 insertions insertion NNS cord-269867-k10siwur 181 38 surrounding surround VBG cord-269867-k10siwur 181 39 the the DT cord-269867-k10siwur 181 40 signal signal JJ cord-269867-k10siwur 181 41 peptide peptide NN cord-269867-k10siwur 181 42 - - HYPH cord-269867-k10siwur 181 43 mature mature JJ cord-269867-k10siwur 181 44 protein protein NN cord-269867-k10siwur 181 45 junction junction NN cord-269867-k10siwur 181 46 of of IN cord-269867-k10siwur 181 47 the the DT cord-269867-k10siwur 181 48 CIpG CIpG NNP cord-269867-k10siwur 181 49 prepilin prepilin NN cord-269867-k10siwur 181 50 : : : cord-269867-k10siwur 181 51 CS31A CS31A NNP cord-269867-k10siwur 181 52 fimbriae fimbriae NNP cord-269867-k10siwur 181 53 of of IN cord-269867-k10siwur 181 54 Escherichia Escherichia NNP cord-269867-k10siwur 181 55 coli coli NNS cord-269867-k10siwur 181 56 as as IN cord-269867-k10siwur 181 57 carriers carrier NNS cord-269867-k10siwur 181 58 of of IN cord-269867-k10siwur 181 59 foreign foreign JJ cord-269867-k10siwur 181 60 sequences sequence NNS cord-269867-k10siwur 181 61 Residues residue NNS cord-269867-k10siwur 181 62 involved involve VBN cord-269867-k10siwur 181 63 in in IN cord-269867-k10siwur 181 64 the the DT cord-269867-k10siwur 181 65 formation formation NN cord-269867-k10siwur 181 66 of of IN cord-269867-k10siwur 181 67 the the DT cord-269867-k10siwur 181 68 antigenic antigenic JJ cord-269867-k10siwur 181 69 sites site NNS cord-269867-k10siwur 181 70 of of IN cord-269867-k10siwur 181 71 the the DT cord-269867-k10siwur 181 72 S S NNP cord-269867-k10siwur 181 73 protein protein NN cord-269867-k10siwur 181 74 of of IN cord-269867-k10siwur 181 75 transmissible transmissible JJ cord-269867-k10siwur 181 76 gastroenteritis gastroenteritis JJ cord-269867-k10siwur 181 77 coronavirus coronavirus NN cord-269867-k10siwur 181 78 Sequence sequence NN cord-269867-k10siwur 181 79 analysis analysis NN cord-269867-k10siwur 181 80 of of IN cord-269867-k10siwur 181 81 the the DT cord-269867-k10siwur 181 82 clpG clpG NNS cord-269867-k10siwur 181 83 gene gene NN cord-269867-k10siwur 181 84 , , , cord-269867-k10siwur 181 85 which which WDT cord-269867-k10siwur 181 86 codes code VBZ cord-269867-k10siwur 181 87 for for IN cord-269867-k10siwur 181 88 surface surface NN cord-269867-k10siwur 181 89 antigen antigen NNP cord-269867-k10siwur 181 90 CS31A cs31a NN cord-269867-k10siwur 181 91 subunit subunit NN cord-269867-k10siwur 181 92 : : : cord-269867-k10siwur 181 93 evidence evidence NN cord-269867-k10siwur 181 94 of of IN cord-269867-k10siwur 181 95 an an DT cord-269867-k10siwur 181 96 evolutionary evolutionary JJ cord-269867-k10siwur 181 97 relationship relationship NN cord-269867-k10siwur 181 98 between between IN cord-269867-k10siwur 181 99 CS31A cs31a NN cord-269867-k10siwur 181 100 , , , cord-269867-k10siwur 181 101 K88 K88 NNP cord-269867-k10siwur 181 102 and and CC cord-269867-k10siwur 181 103 F41 F41 NNP cord-269867-k10siwur 181 104 subunit subunit NN cord-269867-k10siwur 181 105 genes gene NNS cord-269867-k10siwur 181 106 CS31A cs31a NN cord-269867-k10siwur 181 107 , , , cord-269867-k10siwur 181 108 a a DT cord-269867-k10siwur 181 109 new new JJ cord-269867-k10siwur 181 110 K88-related K88-related NNP cord-269867-k10siwur 181 111 fimbrial fimbrial JJ cord-269867-k10siwur 181 112 antigen antigen NN cord-269867-k10siwur 181 113 on on IN cord-269867-k10siwur 181 114 bovine bovine JJ cord-269867-k10siwur 181 115 enterotoxigenic enterotoxigenic NNP cord-269867-k10siwur 181 116 and and CC cord-269867-k10siwur 181 117 septicemic septicemic JJ cord-269867-k10siwur 181 118 Escherichia Escherichia NNP cord-269867-k10siwur 181 119 coli coli VBZ cord-269867-k10siwur 181 120 strains strain NNS cord-269867-k10siwur 182 1 A a DT cord-269867-k10siwur 182 2 simple simple JJ cord-269867-k10siwur 182 3 and and CC cord-269867-k10siwur 182 4 efficient efficient JJ cord-269867-k10siwur 182 5 method method NN cord-269867-k10siwur 182 6 for for IN cord-269867-k10siwur 182 7 the the DT cord-269867-k10siwur 182 8 oligodeoxyribonucleotide oligodeoxyribonucleotide NN cord-269867-k10siwur 182 9 - - HYPH cord-269867-k10siwur 182 10 directed direct VBN cord-269867-k10siwur 182 11 mutagenesis mutagenesis NN cord-269867-k10siwur 182 12 of of IN cord-269867-k10siwur 182 13 double double RB cord-269867-k10siwur 182 14 - - HYPH cord-269867-k10siwur 182 15 stranded strand VBN cord-269867-k10siwur 182 16 plasmid plasmid NN cord-269867-k10siwur 182 17 DNA DNA NNP cord-269867-k10siwur 182 18 Construction Construction NNP cord-269867-k10siwur 182 19 , , , cord-269867-k10siwur 182 20 expression expression NN cord-269867-k10siwur 182 21 and and CC cord-269867-k10siwur 182 22 immunogenicity immunogenicity NN cord-269867-k10siwur 182 23 of of IN cord-269867-k10siwur 182 24 multiple multiple JJ cord-269867-k10siwur 182 25 tandem tandem NN cord-269867-k10siwur 182 26 copies copy NNS cord-269867-k10siwur 182 27 of of IN cord-269867-k10siwur 182 28 the the DT cord-269867-k10siwur 182 29 Schistosoma Schistosoma NNP cord-269867-k10siwur 182 30 mansoni mansoni NN cord-269867-k10siwur 182 31 peptide peptide NN cord-269867-k10siwur 182 32 115 115 CD cord-269867-k10siwur 182 33 - - SYM cord-269867-k10siwur 182 34 131 131 CD cord-269867-k10siwur 182 35 of of IN cord-269867-k10siwur 182 36 the the DT cord-269867-k10siwur 182 37 P28 P28 NNP cord-269867-k10siwur 182 38 glutathione glutathione NN cord-269867-k10siwur 182 39 S s NN cord-269867-k10siwur 182 40 - - HYPH cord-269867-k10siwur 182 41 transferase transferase NNP cord-269867-k10siwur 182 42 expressed express VBD cord-269867-k10siwur 182 43 as as IN cord-269867-k10siwur 182 44 C c NN cord-269867-k10siwur 182 45 - - HYPH cord-269867-k10siwur 182 46 terminal terminal NN cord-269867-k10siwur 182 47 fusions fusion NNS cord-269867-k10siwur 182 48 to to IN cord-269867-k10siwur 182 49 tetanus tetanus NN cord-269867-k10siwur 182 50 toxin toxin NN cord-269867-k10siwur 182 51 fragment fragment NN cord-269867-k10siwur 182 52 C c NN cord-269867-k10siwur 182 53 in in IN cord-269867-k10siwur 182 54 a a DT cord-269867-k10siwur 182 55 live live JJ cord-269867-k10siwur 182 56 Aro aro RB cord-269867-k10siwur 182 57 - - HYPH cord-269867-k10siwur 182 58 attenuated attenuated JJ cord-269867-k10siwur 182 59 vaccine vaccine NN cord-269867-k10siwur 182 60 strain strain NN cord-269867-k10siwur 182 61 of of IN cord-269867-k10siwur 182 62 Salmonella Salmonella NNP cord-269867-k10siwur 182 63 Expression Expression NNP cord-269867-k10siwur 182 64 of of IN cord-269867-k10siwur 182 65 heterologous heterologous JJ cord-269867-k10siwur 182 66 peptides peptide NNS cord-269867-k10siwur 182 67 at at IN cord-269867-k10siwur 182 68 two two CD cord-269867-k10siwur 182 69 permissive permissive JJ cord-269867-k10siwur 182 70 sites site NNS cord-269867-k10siwur 182 71 of of IN cord-269867-k10siwur 182 72 the the DT cord-269867-k10siwur 182 73 MalE MalE NNP cord-269867-k10siwur 182 74 protein protein NN cord-269867-k10siwur 182 75 : : : cord-269867-k10siwur 182 76 antigenicity antigenicity NN cord-269867-k10siwur 182 77 and and CC cord-269867-k10siwur 182 78 immunogenicity immunogenicity NN cord-269867-k10siwur 182 79 of of IN cord-269867-k10siwur 182 80 foreign foreign JJ cord-269867-k10siwur 182 81 B b NN cord-269867-k10siwur 182 82 - - HYPH cord-269867-k10siwur 182 83 cell cell NN cord-269867-k10siwur 182 84 and and CC cord-269867-k10siwur 182 85 T t NN cord-269867-k10siwur 182 86 - - HYPH cord-269867-k10siwur 182 87 cell cell NN cord-269867-k10siwur 182 88 epitopes epitope NNS cord-269867-k10siwur 182 89 Hydrophobic hydrophobic JJ cord-269867-k10siwur 182 90 cluster cluster NN cord-269867-k10siwur 182 91 analysis analysis NN cord-269867-k10siwur 182 92 and and CC cord-269867-k10siwur 182 93 secondary secondary JJ cord-269867-k10siwur 182 94 structure structure NN cord-269867-k10siwur 182 95 predictions prediction NNS cord-269867-k10siwur 182 96 revealed reveal VBD cord-269867-k10siwur 182 97 that that IN cord-269867-k10siwur 182 98 major major JJ cord-269867-k10siwur 182 99 and and CC cord-269867-k10siwur 182 100 minor minor JJ cord-269867-k10siwur 182 101 structural structural JJ cord-269867-k10siwur 182 102 subunits subunit NNS cord-269867-k10siwur 182 103 of of IN cord-269867-k10siwur 182 104 K88-related K88-related NNP cord-269867-k10siwur 182 105 adhesins adhesin NNS cord-269867-k10siwur 182 106 of of IN cord-269867-k10siwur 182 107 Escherichia Escherichia NNP cord-269867-k10siwur 182 108 coli coli NNS cord-269867-k10siwur 182 109 share share VBP cord-269867-k10siwur 182 110 a a DT cord-269867-k10siwur 182 111 common common JJ cord-269867-k10siwur 182 112 overall overall JJ cord-269867-k10siwur 182 113 fold fold NN cord-269867-k10siwur 182 114 and and CC cord-269867-k10siwur 182 115 differ differ VBP cord-269867-k10siwur 182 116 structurally structurally RB cord-269867-k10siwur 182 117 from from IN cord-269867-k10siwur 182 118 other other JJ cord-269867-k10siwur 182 119 fimbrial fimbrial JJ cord-269867-k10siwur 182 120 subunits subunit NNS cord-269867-k10siwur 182 121 Role role NN cord-269867-k10siwur 182 122 of of IN cord-269867-k10siwur 182 123 proteolytic proteolytic JJ cord-269867-k10siwur 182 124 processing processing NN cord-269867-k10siwur 182 125 of of IN cord-269867-k10siwur 182 126 viral viral JJ cord-269867-k10siwur 182 127 envelope envelope NN cord-269867-k10siwur 182 128 glycoproteins glycoprotein NNS cord-269867-k10siwur 182 129 Vaccine Vaccine NNP cord-269867-k10siwur 182 130 technologies technology NNS cord-269867-k10siwur 182 131 : : : cord-269867-k10siwur 183 1 view view VB cord-269867-k10siwur 183 2 to to IN cord-269867-k10siwur 183 3 the the DT cord-269867-k10siwur 183 4 future future JJ cord-269867-k10siwur 183 5 DNA dna NN cord-269867-k10siwur 183 6 sequencing sequencing NN cord-269867-k10siwur 183 7 with with IN cord-269867-k10siwur 183 8 chain chain NN cord-269867-k10siwur 183 9 - - HYPH cord-269867-k10siwur 183 10 terminating terminate VBG cord-269867-k10siwur 183 11 inhibitors inhibitor NNS cord-269867-k10siwur 183 12 Efficiency Efficiency NNP cord-269867-k10siwur 183 13 oligonucleotide oligonucleotide RB cord-269867-k10siwur 183 14 - - HYPH cord-269867-k10siwur 183 15 directed direct VBN cord-269867-k10siwur 183 16 construction construction NN cord-269867-k10siwur 183 17 of of IN cord-269867-k10siwur 183 18 mutations mutation NNS cord-269867-k10siwur 183 19 in in IN cord-269867-k10siwur 183 20 expression expression NN cord-269867-k10siwur 183 21 vectors vector NNS cord-269867-k10siwur 183 22 by by IN cord-269867-k10siwur 183 23 the the DT cord-269867-k10siwur 183 24 gapped gap VBN cord-269867-k10siwur 183 25 duplex duplex NN cord-269867-k10siwur 183 26 DNA dna NN cord-269867-k10siwur 183 27 method method NN cord-269867-k10siwur 183 28 using use VBG cord-269867-k10siwur 183 29 alternative alternative JJ cord-269867-k10siwur 183 30 selectable selectable JJ cord-269867-k10siwur 183 31 markers marker NNS cord-269867-k10siwur 183 32 Immunoreactivity Immunoreactivity NNP cord-269867-k10siwur 183 33 of of IN cord-269867-k10siwur 183 34 chimeric chimeric JJ cord-269867-k10siwur 183 35 proteins protein NNS cord-269867-k10siwur 183 36 carrying carry VBG cord-269867-k10siwur 183 37 the the DT cord-269867-k10siwur 183 38 HIV-1 HIV-1 NNP cord-269867-k10siwur 183 39 epitope epitope NN cord-269867-k10siwur 183 40 IGPGRAF IGPGRAF NNP cord-269867-k10siwur 183 41 : : : cord-269867-k10siwur 183 42 correlation correlation NN cord-269867-k10siwur 183 43 between between IN cord-269867-k10siwur 183 44 predicted predict VBN cord-269867-k10siwur 183 45 conformation conformation NN cord-269867-k10siwur 183 46 and and CC cord-269867-k10siwur 183 47 antigenicity antigenicity NN cord-269867-k10siwur 183 48 Electrophoretic electrophoretic JJ cord-269867-k10siwur 183 49 transfer transfer NN cord-269867-k10siwur 183 50 of of IN cord-269867-k10siwur 183 51 proteins protein NNS cord-269867-k10siwur 183 52 from from IN cord-269867-k10siwur 183 53 polyacrylamide polyacrylamide JJ cord-269867-k10siwur 183 54 gels gel NNS cord-269867-k10siwur 183 55 to to IN cord-269867-k10siwur 183 56 nitrocellulose nitrocellulose NN cord-269867-k10siwur 183 57 sheet sheet NN cord-269867-k10siwur 183 58 : : : cord-269867-k10siwur 183 59 procedure procedure NN cord-269867-k10siwur 183 60 and and CC cord-269867-k10siwur 183 61 some some DT cord-269867-k10siwur 183 62 applications application NNS