id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308867-mrtf8l4f	Heaney, Jude	Chapter 6 Low-Density TaqMan® Array Cards for the Detection of Pathogens	2015-12-31		.txt	text/plain	5598	240	39	This chapter describes the development of TaqMan® Array Cards (TACs), technology which allows the detection of multiple pathogens (up to 48 targets) from a single nucleic acid extract, utilising small volumes and real-time PCR. All PCR-based assays, qualitative and quantitative, should meet Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) requirements as detailed in the literature (Bustin et al., 2010 (Bustin et al., , 2013 Johnson, Nour, Nolan, Huggett, & Bustin, 2014; Taylor, Wakem, Dijkman, Alsarraj, & Nguyen, 2010) and comply with the guidelines outlined for the development and validation of diagnostic tests that depend on nucleic acid amplification and detection (Saunders et al., 2013) . However, careful optimisation of TAC such as testing multiple targets for some pathogens within the card (see Figure 2 ), using efficient nucleic acid extraction methods (particularly for samples such as blood which may have a low yield of organism) and increasing the extraction volume (as well as the nucleic acid input volume), may all help to increase the sensitivity to that suitable for use in routine diagnostics laboratories (Diaz et al., 2013) .	./cache/cord-308867-mrtf8l4f.txt	./txt/cord-308867-mrtf8l4f.txt